A composition for nourishing, refining the skin and enhancing the perception of light, and a process for its preparation and use

Abstract

This invention relates to the field of daily chemical technology, and in particular to a composition that nourishes, refines, and enhances skin radiance, its preparation method, and its application. The composition comprises, by weight percentage: 30%–80% of inulin root juice fermentation product; 0.1%–1% of palmitoyl pentapeptide-4; 0.1%–1.2% of nonapeptide-1; and the balance α-glucan oligosaccharide A. This invention, by introducing α-glucan oligosaccharide into the fermentation process of inulin root juice and controlling the pH and fermentation conditions at the fermentation endpoint, makes the fermentation process more stable and controllable. The resulting fermentation product has a significantly increased content of low-molecular-weight active substances, with the molecular weight distribution concentrated in a lower range, thus enhancing its transdermal absorption performance. Simultaneously, the fermentation product synergistically interacts with dimethylsilanol hyaluronic acid ester, β-glucan, and polysaccharide components from the extract of *Opuntia ficus-indica* stem to form a uniform and dense hydrated film on the skin surface and increase the interfacial refractive index, thereby enhancing skin radiance and improving smoothness.

Description

Technical Field

[0001] This invention relates to the field of daily chemical technology, and in particular to a composition that nourishes, refines, and enhances skin radiance, as well as its preparation method and application. Background Technology

[0002] With the development of the cosmetics industry and the continuous improvement of consumers' skincare concepts, multi-functional skincare products have gradually become the mainstream in the market, especially those that combine moisturizing, nourishing, skin-refining, and skin-brightening effects, which have received widespread attention. Among these, the effects of "hydrated and radiant" or "luminous skin" have become one of the important indicators for evaluating the performance of skincare products, which places higher demands on cosmetic formulation design.

[0003] In existing technologies, moisturizers such as hyaluronic acid, glycerin, and butylene glycol are commonly used to achieve skin hydration and radiance by increasing skin moisture content to improve skin feel. However, these ingredients primarily work by absorbing or locking in water, making it difficult to form a stable structural layer with a high refractive index on the skin surface, thus limiting their effectiveness in improving skin radiance. Furthermore, the wide molecular weight distribution of these ingredients means that some high-molecular-weight substances have difficulty penetrating the stratum corneum, resulting in low bioavailability.

[0004] In addition, peptide active ingredients such as palmitoyl tripeptide and acetyl hexapeptide have been widely used in anti-aging skincare products due to their ability to promote collagen production and improve fine lines. However, peptides are relatively sensitive to environmental conditions and are prone to degradation or conformational changes in acidic systems or high temperatures, thus affecting their stability and efficacy. Furthermore, peptides have a limited residence time on the skin surface, and the lack of effective sustained-release or delivery systems also limits their practical application.

[0005] In recent years, the use of microbial fermentation technology to prepare active ingredients has gradually become an important development direction in the cosmetics industry. Through fermentation, macromolecules in plant raw materials can be degraded into smaller active products, such as organic acids, small sugars, and short peptides, thereby improving their bioavailability and transdermal absorption. However, existing fermentation technologies still have several shortcomings, such as: the fermentation process is difficult to control precisely, resulting in uneven molecular weight distribution of the products; the fermentation system is prone to oxidative browning, affecting the product's appearance and stability; and compatibility issues can easily arise when fermentation products are combined with other active ingredients (such as peptides and moisturizers), thus affecting the overall formula stability and efficacy.

[0006] Specifically, plant-based raw materials rich in fructooligosaccharides, such as inulin, are prone to polyphenol oxidation during fermentation, leading to a darkening of the system's color and even precipitation, severely impacting the product's sensory quality. Furthermore, existing technologies often rely on ascorbic acid or chelating agents for stabilization, but these methods have limited stability or can interfere with the formulation system, making it difficult to meet the safety and stability requirements of high-end skincare products.

[0007] On the other hand, while existing technologies include formulations that combine fermentation products, peptides, and moisturizing ingredients, these are mostly simple additives lacking systematic structural design and mechanism regulation, making it difficult to achieve a balance between transdermal absorption, activity protection, and photosensitivity. For example, complex emulsion systems or high electrolyte content can easily lead to system instability or limited release of active ingredients; furthermore, the combination of too many functional ingredients may mask the effects of the core active ingredient, reducing the overall performance of the product.

[0008] Therefore, how to obtain a system that can improve the stability of plant fermentation products, control their molecular weight distribution, synergize with peptide active ingredients and moisturizing systems, and form a structural layer with good optical properties on the skin surface to achieve skin hydration, smoothness and radiance, while ensuring system stability and industrial feasibility, has become an urgent technical problem to be solved in this field. Summary of the Invention

[0009] To address the problems of poor stability, easy browning, uneven molecular weight distribution, insufficient stability of peptide active ingredients, and lack of photosensitivity enhancement mechanism in existing technologies, this invention provides a composition with nourishing, skin-refining, and photosensitivity effects, as well as its preparation method. By studying the fermentation process and cream formulation, the invention achieves a synergistic improvement in the stability of active ingredients, transdermal absorption, and skin radiance.

[0010] To address the aforementioned technical problems, the present invention provides a composition that nourishes, refines, and enhances skin radiance, comprising, by weight percentage:

[0011] 30%–80% of the fermentation products of Jerusalem artichoke root juice;

[0012] 0.1%–1% palmitoyl pentapeptide-4;

[0013] 0.1%–1.2% of nonapeptide-1;

[0014] The remaining α-glucan oligosaccharides;

[0015] in:

[0016] The fermented product of the Jerusalem artichoke root juice is obtained through microbial fermentation, wherein the microorganisms are selected from one or more of the following: lactobacillus, Lactobacillus plantarum, Bifidobacterium, and yeast.

[0017] As one embodiment of the present invention, the method for preparing the fermented product of Jerusalem artichoke root juice is as follows:

[0018] (1) Obtain the original juice of Jerusalem artichoke root and pasteurize it;

[0019] (2) Add oligosaccharides to the pasteurized inulin root juice and inoculate with pre-activated lactobacillus seed culture for pre-culture:

[0020] (3) After the pre-culture is completed, the compound strain is inoculated for fermentation;

[0021] (4) After fermentation, add a compound enzyme for enzymatic hydrolysis;

[0022] (5) Centrifuge the fermentation broth after enzymatic hydrolysis and take the supernatant;

[0023] (6) Adjust the pH of the supernatant to 4.80 and add a preservative system to obtain the fermentation product of Jerusalem artichoke root juice.

[0024] The present invention provides a face cream comprising the aforementioned composition.

[0025] In one embodiment of the present invention, the raw materials for preparing the face cream include:

[0026] Palmitoyl tripeptide-1 0.05–0.1 parts, palmitoyl tripeptide-5 0.05–0.1 parts, acetyl hexapeptide-8 0.05–0.1 parts, carnosine 0.1–0.2 parts, emulsifier 5–10 parts, moisturizer 2–4 parts, plant extract 1–5 parts, preservative 0.5–1 part, a composition that nourishes, refines, and enhances skin radiance 1–10 parts, water to make up to 100 parts.

[0027] In one embodiment of the present invention, the emulsifier is PEG-100 stearate, glyceryl stearate, cetearyl alcohol, squalane, and avocado butter.

[0028] In one embodiment of the present invention, the moisturizer is glycerin, butylene glycol, trehalose, or dimethylsilanol hyaluronic acid ester.

[0029] In one embodiment of the present invention, the plant extract is an extract of Prunus persica cactus, a pomegranate extract, and a Centella asiatica extract.

[0030] As one embodiment of the present invention, the preparation method of the face cream includes the following steps:

[0031] Step 1: A composition that nourishes, refines, and enhances skin radiance.

[0032] Step 2: Preparation of emulsion phase

[0033] PEG-100 stearate, glyceryl stearate, avocado butter, squalane, and cetearyl alcohol are mixed and heated to 70°C to obtain the oil phase; glycerol, butylene glycol, trehalose, dimethylsilane hyaluronic acid ester, and buffer are mixed and heated to 70°C to obtain the aqueous phase; the aqueous phase is slowly added to the oil phase, stirred evenly, and cooled to below 40°C.

[0034] Step 3: Add the composition obtained in Step 1 to the emulsion phase obtained in Step 2, and gently stir until uniformly dispersed.

[0035] Step 4: Add phenoxyethanol, ethylhexylglycerin, potassium sorbate, and plant extracts, and adjust the pH to 5.2–5.5.

[0036] Step 5: Then, perform high-shear homogenization to obtain a stable and uniform face cream.

[0037] As one embodiment of the present invention, the composition is applied in the field of daily chemical products.

[0038] By adopting the above technical solution, the present invention has the following beneficial effects:

[0039] This invention introduces α-glucan oligosaccharides into the fermentation process of inulin root juice for pre-culturing and controls the pH and fermentation conditions at the fermentation endpoint, making the fermentation process more stable and controllable. The resulting fermentation product exhibits a significantly increased content of low-molecular-weight active substances with a molecular weight distribution concentrated in a lower range, enhancing its transdermal absorption performance. Simultaneously, the fermentation product synergistically interacts with dimethylsilanol hyaluronic acid ester, β-glucan, and polysaccharide components from the extract of *Opuntia ficus-indica* stem to form a uniform and dense hydrated film on the skin surface and increase interfacial refractive index, thereby enhancing skin radiance and improving smoothness. Furthermore, fermentation regulation reduces the occurrence of polyphenol oxidation, lowering the risk of browning and improving product appearance stability. This invention also regulates the skin's microecological environment through the synergistic effect of α-glucan oligosaccharides and fermentation products, enhancing skin barrier function and improving tolerance. Attached Figure Description

[0040] To more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the specific embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of the present invention. For those skilled in the art, other drawings can be obtained from these drawings without creative effort.

[0041] Figure 1This is the product diagram corresponding to Example 1. Detailed Implementation

[0042] The present invention will be further explained below with reference to specific embodiments.

[0043] This invention provides a composition that nourishes, refines, and enhances skin radiance, comprising, by weight percentage:

[0044] 30%–80% of the fermentation products of Jerusalem artichoke root juice;

[0045] 0.1%–1% palmitoyl pentapeptide-4;

[0046] 0.1%–1.2% of nonapeptide-1;

[0047] The remaining amount of α-glucan oligosaccharide B;

[0048] The preparation method of the fermented product of Jerusalem artichoke root juice is as follows:

[0049] (1) Raw material pretreatment

[0050] Fresh Jerusalem artichoke tubers were taken, peeled, and washed with deionized water at 20°C for 3 minutes. The washed Jerusalem artichoke tubers were then cut into 3 mm particles. A pulping machine was then used to prepare the pulp at 3000 rpm for 5 minutes to obtain a uniform Jerusalem artichoke pulp. The pulp was then filtered through a 120-mesh sieve to obtain the Jerusalem artichoke root juice concentrate.

[0051] (2) Sterilization treatment

[0052] The pretreated Jerusalem artichoke root juice was pasteurized at 95°C for 10 minutes and then rapidly cooled to 37°C for later use.

[0053] (3) Add α-glucan oligosaccharide A to the cooled inulin root juice, controlling the addition amount to 1.00 wt%;

[0054] Subsequently, inoculate with pre-activated Lactobacillus seed culture (live bacteria concentration of 10). 8 The inoculum was 1.00 wt% (CFU / mL); the pre-culture was carried out under the following conditions: temperature: 37℃, time: 6 h, stirring speed: 100 rpm, and the pH of the system was controlled to decrease naturally from the initial 6.20 to 5.20 (±0.10) during the pre-culture.

[0055] (4) Inoculation and fermentation

[0056] After the pre-culture was completed, the compound bacterial strain was inoculated again, with the total inoculation amount controlled at 2.00 wt%, of which:

[0057] Lactobacillus: 1.00 wt%, Yeast: 0.70 wt%, Bifidobacterium: 0.30 wt%.

[0058] The fermentation conditions were controlled as follows: temperature: 37℃, stirring speed: 120 rpm, fermentation time: 30 h; and the fermentation endpoint was controlled as follows: pH: 4.50 (±0.10).

[0059] (5) Enzymatic hydrolysis and synergistic degradation

[0060] After fermentation, a compound enzyme system was added for further degradation: inulinase: 0.30 wt%, protease: 0.10 wt%; enzymatic hydrolysis was carried out at 45℃ for 2 h; after enzymatic hydrolysis, the temperature was raised to 85℃ and held for 10 min to inactivate the enzyme.

[0061] (6) Separation and purification

[0062] The fermentation broth after enzymatic hydrolysis was centrifuged at 8000 rpm for 15 min. The supernatant was then filtered through a 0.22 μm microporous membrane and subsequently fractionated using an ultrafiltration membrane with a molecular weight cutoff of 3 kDa. The permeate was collected as the low molecular weight fermentation product.

[0063] (7) Final adjustment and stabilization

[0064] The obtained fermentation product was subjected to final conditioning treatment: pH was adjusted to 4.80 (±0.05); 0.50 wt% 1,2-hexanediol was added; and the product was stored under dark conditions to obtain a stable inulin root juice fermentation product.

[0065] This invention introduces an α-glucan oligosaccharide pre-culture regulation step into the inulin root juice fermentation system, combined with subsequent fermentation, enzymatic hydrolysis, and molecular weight fractionation, to significantly increase and stably control the content of low-molecular-weight active substances in the resulting product within a lower molecular weight range, thereby effectively enhancing its transdermal absorption performance. Furthermore, the fermentation product forms a uniform and dense hydrated film on the skin surface and increases the interfacial refractive index, resulting in excellent skin luster and smoothness. Compared with existing technologies, this invention exhibits significant advantages in inhibiting browning, improving system stability, enhancing the skin's microecological environment, and improving product sensory properties. Moreover, the process conditions are mild and reproducible, making it suitable for industrial production and application in various types of cosmetics.

[0066] The preparation method of the composition that nourishes, refines the skin and enhances radiance is as follows: the fermented product of inulin root juice is cooled to below 40°C, and the following components are added in sequence: palmitoyl pentapeptide-4 and nonapeptide-1; and the mixture is stirred at low speed (50-100 rpm) to make it evenly dispersed; α-glucan oligosaccharide B is added, and stirring is continued until it is completely dissolved to obtain a homogeneous mixture.

[0067] This invention provides a face cream, the raw materials for which the face cream is prepared include:

[0068] Palmitoyl tripeptide-1 0.05–0.1 parts, palmitoyl tripeptide-5 0.05–0.1 parts, acetyl hexapeptide-8 0.05–0.1 parts, carnosine 0.1–0.2 parts, emulsifier 5–10 parts, moisturizer 2–4 parts, plant extract 1–5 parts, preservative 0.5–1 part, a composition that nourishes, refines, and enhances skin radiance 1–10 parts, water to make up to 100 parts.

[0069] In one embodiment of the present invention, the emulsifier is PEG-100 stearate, glyceryl stearate, cetearyl alcohol, squalane, and avocado butter in a mass ratio of 1:1.5:2:2:1.

[0070] In one embodiment of the present invention, the moisturizer is glycerin, butylene glycol, trehalose, and dimethylsilane hyaluronic acid ester in a mass ratio of 5:3:1:0.2.

[0071] In one embodiment of the present invention, the plant extracts are Prunus persica cactus extract, pomegranate extract and Centella asiatica extract, in a mass ratio of 3:2:1.

[0072] As one embodiment of the present invention, the preparation method of the face cream includes the following steps:

[0073] Step 1: Prepare a composition that nourishes, refines, and enhances skin radiance.

[0074] Step 2: Preparation of emulsion phase

[0075] PEG-100 stearate, glyceryl stearate, avocado butter, squalane, and cetearyl alcohol are mixed and heated to 70°C to obtain the oil phase; glycerol, butylene glycol, trehalose, dimethylsilane hyaluronic acid ester, and buffer are mixed and heated to 70°C to obtain the aqueous phase; the aqueous phase is slowly added to the oil phase, stirred evenly, and cooled to below 40°C.

[0076] Step 3: Add the composition obtained in Step 1 to the emulsion phase obtained in Step 2, and gently stir until uniformly dispersed.

[0077] Step 4: Add the remaining components and adjust the pH to 5.2–5.5.

[0078] Step 5: Then, perform high-shear homogenization to obtain a stable and uniform face cream.

[0079] The face cream of this invention innovates in its formulation structure and mechanism of action, and is not a simple superposition of existing multi-component systems. Specifically, this invention uses low-molecular-weight inulin fermented root juice as the core active ingredient. Through pre-cultivation regulation and fermentation by compound bacteria combined with enzymatic hydrolysis and fractionation, the molecular weight of the active substances is concentrated below 3 kDa, thereby significantly improving transdermal absorption capacity and forming a uniform hydration film layer on the skin surface to enhance interfacial refractive index. Furthermore, palmitoyl pentapeptide-4, nonapeptide-1, and a polypeptide complex system are introduced using a low-temperature post-addition method, effectively avoiding polypeptide inactivation in high-temperature and acidic environments, and improving the sustainability of anti-wrinkle and repair effects. Simultaneously, a layered liquid crystal emulsion structure is constructed using a specific ratio of PEG-100 stearate, glyceryl stearate, cetearyl alcohol, squalane, and avocado butter, allowing the oil phase to form a continuous and uniform microscopic arrangement on the skin surface, from... This invention enhances the spreadability and uniformity of light reflection. Furthermore, the multi-level moisturizing system formed by glycerin, butylene glycol, trehalose, and dimethylsilanol hyaluronic acid ester works synergistically with polysaccharide components such as prickly pear cactus extract to further strengthen skin surface hydration and improve micro-roughness. Pomegranate polyphenols and centella asiatica active ingredients from plant extracts respectively exert antioxidant and barrier repair effects, thereby stabilizing the system while improving skin smoothness and radiance. Therefore, this invention's face cream, while achieving nourishing, moisturizing, and anti-wrinkle effects, can significantly improve skin's radiance and translucency.

[0080] The following detailed description is based on specific embodiments.

[0081] Example 1

[0082] This embodiment provides a composition that nourishes, refines, and enhances skin radiance, comprising, by weight percentage:

[0083] 60% of the fermented product of Jerusalem artichoke root juice;

[0084] 0.5% palmitoyl pentapeptide-4;

[0085] 0.5% nonapeptide-1;

[0086] The remaining amount of α-glucan oligosaccharide B;

[0087] The preparation method of the fermented product of Jerusalem artichoke root juice is as follows:

[0088] (1) Raw material pretreatment

[0089] Fresh Jerusalem artichoke tubers were taken, peeled, and washed with deionized water at 20°C for 3 minutes. The washed Jerusalem artichoke tubers were then cut into 3 mm particles. A pulping machine was then used to prepare the pulp at 3000 rpm for 5 minutes to obtain a uniform Jerusalem artichoke pulp. The pulp was then filtered through a 120-mesh sieve to obtain the Jerusalem artichoke root juice concentrate.

[0090] (2) Sterilization treatment

[0091] The pretreated Jerusalem artichoke root juice was pasteurized at 95°C for 10 minutes and then rapidly cooled to 37°C for later use.

[0092] (3) Add α-glucan oligosaccharide A to the cooled inulin root juice, controlling the addition amount to 1.00 wt%;

[0093] Subsequently, inoculate with pre-activated Lactobacillus seed culture (live bacteria concentration of 10). 8 The inoculum was 1.00 wt% (CFU / mL); the pre-culture was carried out under the following conditions: temperature: 37℃, time: 6 h, stirring speed: 100 rpm, and the pH of the system was controlled to decrease naturally from the initial 6.20 to 5.20 (±0.10) during the pre-culture.

[0094] (4) Inoculation and fermentation

[0095] After the pre-culture was completed, the compound bacterial strain was inoculated again, with the total inoculation amount controlled at 2.00 wt%, of which:

[0096] Lactobacillus: 1.00 wt%, Yeast: 0.70 wt%, Bifidobacterium: 0.30 wt%.

[0097] The fermentation conditions were controlled as follows: temperature: 37℃, stirring speed: 120 rpm, fermentation time: 30 h; and the fermentation endpoint was controlled as follows: pH: 4.50 (±0.10).

[0098] (5) Enzymatic hydrolysis

[0099] After fermentation, a compound enzyme system was added for further degradation: inulinase: 0.30 wt%, protease: 0.10 wt%; enzymatic hydrolysis was carried out at 45℃ for 2 h; after enzymatic hydrolysis, the temperature was raised to 85℃ and held for 10 min to inactivate the enzyme.

[0100] (6) Separation and purification

[0101] The fermentation broth after enzymatic hydrolysis was centrifuged at 8000 rpm for 15 min. The supernatant was then filtered through a 0.22 μm microporous membrane and subsequently fractionated using an ultrafiltration membrane with a molecular weight cutoff of 3 kDa. The permeate was collected as the low molecular weight fermentation product.

[0102] (7) Final adjustment and stabilization

[0103] The obtained fermentation product was subjected to final conditioning treatment: pH was adjusted to 4.80 (±0.05); 0.50 wt% 1,2-hexanediol was added; and the product was stored under dark conditions to obtain a stable inulin root juice fermentation product.

[0104] The fermented product of Jerusalem artichoke root juice was cooled to below 40°C, and the following components were added in sequence: palmitoyl pentapeptide-4 and nonapeptide-1; and stirred at low speed (60 rpm) to make it evenly dispersed; α-glucan oligosaccharide B was added, and stirring was continued until completely dissolved to obtain a homogeneous mixture.

[0105] This embodiment also provides a face cream, the raw materials for which the face cream is prepared include:

[0106] Palmitoyl tripeptide-1 0.08 parts, palmitoyl tripeptide-5 0.08 parts, acetyl hexapeptide-8 0.08 parts, carnosine 0.15 parts, emulsifier 8 parts, moisturizer 3 parts, plant extract 3 parts, preservative 0.5 parts, a composition that nourishes, refines, and enhances skin radiance 5 parts, water to make up to 100 parts.

[0107] The emulsifiers are PEG-100 stearate, glyceryl stearate, cetearyl alcohol, squalane, and avocado butter in a mass ratio of 1:1.5:2:2:1.

[0108] The moisturizer is composed of glycerin, butylene glycol, trehalose, and dimethylsilanol hyaluronic acid ester in a mass ratio of 5:3:1:0.2.

[0109] The dimethylsilanol hyaluronic acid ester is designated as Sanocon® HASH-100.

[0110] The plant extracts are Prunus persica cactus extract, pomegranate extract, and Centella asiatica extract, in a mass ratio of 3:2:1.

[0111] The preservatives are phenoxyethanol and ethylhexylglycerin in a ratio of 5:2.

[0112] The preparation method of the face cream is as follows:

[0113] Step 1: Prepare a composition that nourishes, refines, and enhances skin radiance.

[0114] Step 2: Preparation of emulsion phase

[0115] PEG-100 stearate, glyceryl stearate, avocado butter, squalane, and cetearyl alcohol are mixed and heated to 70°C to obtain the oil phase; glycerol, butylene glycol, trehalose, dimethylsilane hyaluronic acid ester, and buffer are mixed and heated to 70°C to obtain the aqueous phase; the aqueous phase is slowly added to the oil phase, stirred evenly, and cooled to below 40°C.

[0116] Step 3: Add the composition obtained in Step 1 to the emulsion phase obtained in Step 2, and gently stir until uniformly dispersed.

[0117] Step 4: Add the remaining components and adjust the pH to 5.3.

[0118] Step 5: Then, perform high-shear homogenization to obtain a stable and uniform face cream.

[0119] Comparative Example 1

[0120] The difference from Example 1 is that α-glucan oligosaccharide A is not added during fermentation.

[0121] Comparative Example 2

[0122] The difference from Example 1 is that the step of "after fermentation, adding a compound enzyme system for further degradation: inulinase: 0.30 wt%, protease: 0.10 wt%; enzymatic hydrolysis at 45°C for 2 h; after enzymatic hydrolysis, raising the temperature to 85°C and holding for 10 min to inactivate the enzyme" is omitted, and subsequent steps are performed directly.

[0123] Comparative Example 3

[0124] The difference from Example 1 is that the step of "separation and purification" is omitted.

[0125] The enzymatically hydrolyzed fermentation broth was centrifuged at 8000 rpm for 15 min. The supernatant was then filtered through a 0.22 μm microporous membrane and subsequently fractionated using ultrafiltration, with a molecular weight cutoff of 3 kDa. The permeate was collected as the low-molecular-weight fermentation product.

[0126] Comparative Example 4

[0127] The difference from Example 1 is that the mass ratio of PEG-100 stearate, glyceryl stearate, cetearyl alcohol, squalane, and avocado butter is 1:1:1:1:1.

[0128] Comparative Example 5

[0129] The difference from Example 1 is that no plant extracts are used.

[0130] Comparative Example 6

[0131] The difference from Example 1 is that the moisturizers are only glycerin and butylene glycol.

[0132] Performance testing

[0133] 1. Low molecular weight distribution test

[0134] Gel permeation chromatography (GPC) was used for testing. The column was TSKgel G2000SWXL, the mobile phase was PBS buffer (pH=7.0), the flow rate was 0.5 mL / min, the detection wavelength was 210 nm, and the proportion of components <3 kDa was recorded.

[0135] 2. Transdermal absorption test

[0136] 2.1 Skin preparation

[0137] Select fresh, detached pig ear skin or back skin, and process it as follows:

[0138] Remove subcutaneous fat tissue, preserve the intact stratum corneum, rinse thoroughly with physiological saline, and trim the thickness to approximately 500–800 μm;

[0139] 2.2 Sample Preparation

[0140] Take 0.50 g of the sample from the examples and comparative examples for later use.

[0141] Preparation of receiving solution: PBS buffer (pH=7.4), add 20% (v / v) ethanol to enhance solubility, pre-equilibrate at 32℃ for 30 min before use.

[0142] Franz diffusion cell setup prepared, diffusion area: 1.77 cm² 2 Receiver volume: approximately 12 mL, temperature control: 32±0.5℃, stirring speed: 300 rpm.

[0143] The treated pigskin was placed flat between the diffusion chambers, with the stratum corneum facing the donor chamber (upper layer) and the dermis facing the receiver liquid (lower layer), ensuring no air bubbles or wrinkles, and secured with clamps. Preheated receiver liquid was added to the receiver chamber, and the temperature and stirring system was turned on, allowing it to equilibrate for 30 minutes to stabilize the skin. Then, 0.50 g of sample was evenly spread in the donor chamber using a spatula, avoiding localized accumulation, and the donor chamber was sealed to prevent evaporation. Samples were then taken at 2 h, 4 h, 8 h, and 12 h. 0.50 mL of sample was removed from the receiver chamber, and an equal volume of fresh receiver liquid (32℃) was added. The sample was filtered (using a 0.22 μm filter membrane) for later use. The permeate content was determined using high-performance liquid chromatography (HPLC): column: C18 reversed-phase column; mobile phase: water / acetonitrile system; detection wavelength: 210 nm. A standard curve was established, and the concentration was calculated. Higher permeate volume indicates stronger transdermal absorption; a smoother curve indicates better release stability.

[0144] Subject preparation

[0145] Select 35 healthy volunteers (aged 18-50)

[0146] Avoid using any skincare products 24 hours before the test.

[0147] Before testing, allow the sample to stand for 30 minutes in a constant temperature and humidity environment (22℃, 50% RH).

[0148] 3. Skin luster test

[0149] 3.1 Test Procedure

[0150] A test area (approximately 3 cm × 3 cm) was marked on the inner side of the forearm, and the initial gloss value (denoted as G0) was recorded. 0.05 g of sample was taken and evenly applied, and gently applied until absorbed (approximately 1 min). The gloss was measured 8 h later (denoted as G8). The average value of each sample was taken from 35 volunteers.

[0151] 3.2 Data Calculation

[0152] Gloss enhancement rate = (G8 - G0) / G0 * 100%

[0153] 4. Moisturizing performance test

[0154] 4.1. Test Conditions

[0155] Instrument: Corneometer

[0156] Environment: 22±1℃, 50±5% RH

[0157] 4.2. Test Procedure

[0158] Select a test area on the forearm, measure the initial moisture value (T0), apply 0.05 g of sample, and measure the moisture value (T2) after 2 hours. Take 3 measurements at each point and average the results.

[0159] 4.3. Data Calculation

[0160] Moisture retention rate (%) = T2 / T0 * 100%

[0161] 5. Skin smoothness test (VISIA)

[0162] 5.1. Test Preparation

[0163] Instrument: VISIA Skin Analysis System

[0164] The subjects were divided into 7 groups of 5 people each. After cleaning their skin, they were left to rest for 30 minutes.

[0165] 5.2. Test Procedure

[0166] Before use, take a skin image (baseline). Apply a sample (0.05 g) and use it continuously for 7 days. Take another skin image and analyze the texture uniformity parameter (T value) using software.

[0167] 5.3. Data Calculation

[0168] Fineness improvement rate (%) = (T1 - T0) / T0 * 100%

[0169] 6. Stability Testing

[0170] 6.1. Accelerated Experimental Conditions

[0171] Temperature: 45±1℃; Time: 30 days

[0172] Simultaneously, hot and cold cycling tests were conducted: -5℃ (24 h) and 40℃ (24 h), for a total of 5 cycles.

[0173] 6.2. Testing Procedure

[0174] (1) Visual observation

[0175] Whether it separates into layers, whether it separates into oil, whether it is cloudy

[0176] (2) Color difference measurement

[0177] Instrument: Colorimeter (CIELAB system), used to measure L*, a*, and b* values.

[0178] 6.3. Data Calculation

[0179]

[0180] Where: L0, a0, b0: initial color; L, a, b: changed color.

[0181] 6.4. Judgment Criteria

[0182] ΔE < 1.5: Stable

[0183] ΔE > 3: Significant change

[0184] Layering occurs: Unstable

[0185] 7. Antioxidant Capacity Test (DPPH)

[0186] 7.1. Reagent Preparation

[0187] DPPH solution: 0.1 mmol / L (prepared with ethanol)

[0188] Sample solution: diluted appropriately

[0189] 7.2. Test Procedure

[0190] Take 2.0 mL of DPPH solution, add 1.0 mL of sample solution, mix well, react in the dark for 30 min, and measure the absorbance at 517 nm.

[0191] Three sets of data were measured: A0: blank (DPPH + ethanol), A1: sample + DPPH, and A2: sample blank.

[0192] 7.3. Data Calculation

[0193] Clearance rate (%) = (1 - (A1 - A2) / A0) * 100%.

[0194] A0 = Absorbance of the DPPH solution itself (without sample); A1 = Absorbance of DPPH plus sample; A2 = Absorbance of the sample itself (without DPPH).

[0195] Table 1 Performance Test Results

[0196]

[0197] As shown in the table, Example 1 of the present invention is significantly superior to the comparative example in all performance indicators, especially in terms of low molecular weight active substance content, transdermal absorption performance, and skin gloss. Specifically, the <3 kDa component in Example 1 accounts for 78.5%, and the transdermal absorption rate is increased to 185.6 μg / cm³. 2 Meanwhile, the gloss improvement rate reached 38.2%, which was significantly better than the control group that did not undergo pre-culture, enzymatic hydrolysis or fractionation treatment. This indicates that the present invention has achieved synergistic improvement of multi-dimensional performance through fermentation regulation, low molecular weight treatment and structural optimization design, and has significant technical effects.

[0198] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and not to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some or all of the technical features; and these modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A composition that nourishes, refines, and enhances skin radiance, characterized in that, Included by weight percentage: 30%–80% of the fermentation products of Jerusalem artichoke root juice; 0.1%–1% palmitoyl pentapeptide-4; 0.1%–1.2% of nonapeptide-1; The remaining α-glucan oligosaccharides; in: The fermented product of the Jerusalem artichoke root juice is obtained through microbial fermentation, wherein the microorganisms are selected from one or more of the following: lactobacillus, Lactobacillus plantarum, Bifidobacterium, and yeast.

2. The composition according to claim 1, which nourishes, refines, and enhances skin radiance, is characterized in that... The preparation method of the fermented product of Jerusalem artichoke root juice is as follows: (1) Obtain the original juice of Jerusalem artichoke root and pasteurize it; (2) Add oligosaccharides to the pasteurized inulin root juice and inoculate with pre-activated lactobacillus seed culture for pre-culture: (3) After the pre-culture is completed, the compound strain is inoculated for fermentation; (4) After fermentation, add a compound enzyme for enzymatic hydrolysis; (5) Centrifuge the fermentation broth after enzymatic hydrolysis and take the supernatant; (6) Adjust the pH of the supernatant to 4.80 and add a preservative system to obtain the fermentation product of Jerusalem artichoke root juice.

3. A face cream, characterized in that, It comprises the composition of claim 1.

4. The face cream according to claim 3, characterized in that, The ingredients for preparing the face cream include: Palmitoyl tripeptide-1 0.05–0.1 parts, palmitoyl tripeptide-5 0.05–0.1 parts, acetyl hexapeptide-8 0.05–0.1 parts, carnosine 0.1–0.2 parts, emulsifier 5–10 parts, moisturizer 2–4 parts, plant extract 1–5 parts, preservative 0.5–1 part, composition according to claim 1 1–10 parts, water to make up to 100 parts.

5. The face cream according to claim 4, characterized in that, The emulsifiers are PEG-100 stearate, glyceryl stearate, cetearyl alcohol, squalane, and avocado butter.

6. The face cream according to claim 5, characterized in that, The moisturizers are glycerin, butylene glycol, trehalose, and dimethylsilane hyaluronic acid ester.

7. The face cream according to claim 6, characterized in that, The plant extracts are Prunus persica cactus extract, pomegranate extract, and Centella asiatica extract.

8. The method for preparing face cream according to claim 6, characterized in that, The steps are as follows: Step 1: A composition that nourishes, refines, and enhances skin radiance. Step 2: Preparation of emulsion phase PEG-100 stearate, glyceryl stearate, avocado butter, squalane, and cetearyl alcohol are mixed and heated to 70°C to obtain the oil phase; glycerol, butylene glycol, trehalose, dimethylsilane hyaluronic acid ester, and buffer are mixed and heated to 70°C to obtain the aqueous phase; the aqueous phase is slowly added to the oil phase, stirred evenly, and cooled to below 40°C. Step 3: Add the composition obtained in Step 1 to the emulsion phase obtained in Step 2, and gently stir until uniformly dispersed. Step 4: Add phenoxyethanol, ethylhexylglycerin, potassium sorbate, and plant extracts, and adjust the pH to 5.2–5.

5. Step 5: Then, perform high-shear homogenization to obtain a stable and uniform face cream.

9. The composition according to claim 1 is applied in the field of daily chemical products.

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