Fluorescent probe for detecting peroxynitrite in living cells and synthesis method thereof

By using rhodamine derivative-based fluorescent probes, the problems of insufficient selectivity and response speed of fluorescent probes in existing technologies have been solved, enabling rapid and sensitive detection and imaging of peroxynitrite in living cells.

CN122167380APending Publication Date: 2026-06-09NANJING UNIV OF SCI & TECH
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Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Applications(China)
Current Assignee / Owner
NANJING UNIV OF SCI & TECH
Filing Date
2026-01-19
Publication Date
2026-06-09

AI Technical Summary

Technical Problem

Existing fluorescent probes have poor selectivity and long response time when detecting peroxynitrite in living cells, making it difficult to achieve rapid response and high sensitivity in live cell imaging in complex cellular microenvironments.

Method used

Using a rhodamine derivative as the fluorophore, an amphiphilic ester-water structure of n-dodecylamine propanesulfonate was constructed through an amidation reaction, and 4-amino-2-methoxyphenol was introduced as an ONOO-specific recognition group to prepare a fluorescent probe. This probe releases the fluorophore after reacting with ONOO- to achieve a rapid response.

Benefits of technology

It achieves a rapid response to ONOO- (complete response within 5 s), exhibits high sensitivity and selectivity, and demonstrates low cytotoxicity and good biocompatibility in living cells, enabling it to be used for imaging of both endogenous and exogenous ONOO-.

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Abstract

This invention discloses a fluorescent probe for detecting peroxynitrite in living cells and its synthesis method. The fluorescent probe comprises an amphiphilic group, 4-amino-2-methoxyphenol as a recognition unit, and a rhodamine derivative fluorophore, wherein the recognition unit can interact with ONOO. ‑ A specific oxidation reaction occurs, causing significant changes in fluorescence intensity and emission wavelength, thereby enabling real-time imaging and quantitative analysis of peroxynitrite in the cellular microenvironment. The fluorescent probe of this invention possesses high selectivity, strong anti-interference capabilities, and rapid response, making it suitable for the detection of ONOO at the live-cell level. ‑ Dynamic observation.
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