NiFe-LDH / Zr-MOF electrochemiluminescence immunosensor, preparation method and application thereof

A highly sensitive and stable method for detecting H-FABP was constructed by using NiFe-LDH synergistic electrocatalysis and Zr-MOF coordination aggregation-induced electrochemiluminescence technology. This method solves the sensitivity and stability problems of existing ECL immunosensors and achieves efficient detection of H-FABP.

CN122171649APending Publication Date: 2026-06-09GUANGXI NORMAL UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
GUANGXI NORMAL UNIV
Filing Date
2026-04-09
Publication Date
2026-06-09

AI Technical Summary

Technical Problem

Existing ECL immunosensors suffer from insufficient sensitivity, limited signal amplification, and poor stability, making it difficult to meet the needs of early diagnosis of heart-type fatty acid-binding protein (H-FABP).

Method used

A method for enhancing Zr-MOF coordination aggregation-induced electrochemiluminescence by NiFe-LDH synergistic electrocatalysis was adopted. A recognition layer was formed by modifying the electrode surface with a NiFe-LDH catalyst layer and a Zr-MOF/BSA/Ab1 complex. A sandwich-type immune complex structure was constructed using ZIF-8@CCM@AuNPs/BSA/Ab2 probes, and electrochemiluminescence detection was performed in combination with the co-reactant K2S2O8.

Benefits of technology

It achieves ultrasensitive detection of H-FABP with a low detection limit and a wide linear range. The sensor exhibits good stability under repeated scanning and storage conditions and is suitable for the detection of H-FABP in clinical serum samples.

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Abstract

The application discloses a kind of NiFe-LDH synergistic electrocatalysis enhanced Zr-MOF coordination aggregation induced electrochemiluminescence immunosensor and its preparation method and application, belong to electrochemiluminescence biosensor technical field.The sensor electrode is used as substrate, first NiFe-LDH catalytic layer is constructed on the surface of electrode, then Zr-MOF / BSA / Ab1 recognition layer is modified, then H-FABP is introduced by antigen antibody specific recognition, finally ZIF-8@CCM@AuNPs / BSA / Ab2 quenching probe is added to form sandwich type immune complex structure, and the electrochemiluminescence detection of H-FABP is realized in K2S2O8 co-reaction system.NiFe-LDH can synergistically catalyze activation of persulfate, enhance the electrochemiluminescence signal of Zr-MOF;Zr-MOF is limited by coordination effect Molecular movement improves the stability and luminous efficiency of luminescence.The immunosensor constructed by the application has the advantages of high sensitivity, wide linear range, good repeatability, strong stability and good selectivity, and can be used for quantitative detection of H-FABP in serum sample.
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Claims

1. An electrochemiluminescence detection method for heart-type fatty acid-binding protein, characterized in that, The procedure includes the following steps: modifying the electrode surface with a NiFe-LDH catalyst layer; modifying the NiFe-LDH catalyst layer surface with a Zr-MOF / BSA / Ab1 complex to form a recognition layer; incubating the sample to be tested with the electrode to allow H-FABP in the sample to specifically bind to Ab1; adding a ZIF-8@CCM@AuNPs / BSA / Ab2 probe to the electrode surface and incubating it to allow it to bind with H-FABP to form a sandwich-type immune complex structure; performing electrochemiluminescence detection on the electrode in a buffer solution containing the co-reactant K2S2O8, and quantitatively analyzing H-FABP based on changes in the ECL signal.

2. The method according to claim 1, characterized in that, The concentration of the NiFe-LDH catalyst solution is 0.1 mg·mL. -1 .

3. The method according to claim 1, characterized in that, The Zr-MOF is formed by the coordination of zirconium metal ions with [1,1'-binaphthyl]-4,4'-dicarboxylic acid under solvothermal conditions.

4. The method according to claim 1, characterized in that, The ZIF-8@CCM@AuNPs are formed by encapsulating curcumin in ZIF-8 and loading AuNPs in situ on its surface.

5. The method according to any one of claims 1 to 4, characterized in that, The electrode is a glassy carbon electrode, a gold electrode, a platinum electrode, an indium tin oxide electrode, or an indium tin oxide fluoride electrode.

6. An electrochemiluminescence immunosensor, characterized in that, Prepared by the method according to any one of claims 1 to 5.

7. The application of the electrochemiluminescence immunosensor according to claim 6 in the detection of heart-type fatty acid-binding protein.