A temperature-controlled incubation device and related apparatus

Abstract

The application provides a temperature control culture device and related equipment, and belongs to the technical field of intelligent control. The device comprises a culture reactor, an intelligent controller and a monitoring and executing assembly. The culture reactor is used for simulating a natural environment according to environmental parameters. The intelligent controller is fixed on any side of the culture reactor and is used for generating a control signal of the culture reactor according to multiple environmental parameters output by the monitoring and executing assembly and environmental parameters of a target region obtained through a network. The monitoring and executing assembly is assembled in the culture reactor and comprises a monitoring assembly and an executing assembly. The monitoring assembly is used for monitoring multiple environmental parameters of the culture reactor and sending the monitored multiple environmental parameters to the intelligent controller. The executing assembly is used for adjusting the environmental parameters of the culture reactor according to the control signal output by the intelligent controller. The application improves the accuracy of the culture device in simulation culture, can provide data support for the generation of the control signal by remotely obtaining the environmental parameters of any target region, and can greatly improve the applicability and convenience.

Description

Technical Field

[0001] This invention relates to the field of intelligent control technology, specifically to a temperature-controlled culture device and related equipment. Background Technology

[0002] In the field of ecological and environmental science research, cultivation devices are the core equipment for simulating natural ecosystems and studying the growth patterns of organisms and the correlation between environmental factors.

[0003] Existing culture equipment has low temperature control precision, making it difficult to simulate the dynamic changes in temperature in the natural environment. It relies on constant temperature experiments and cannot simulate the actual temperature fluctuation characteristics in the natural environment, resulting in deviations between environmental research results and actual conditions. The simulation experiments are inaccurate, and the simulation experiments are easily limited by the differences in natural environmental temperature factors in different geographical regions, resulting in poor regional applicability. Summary of the Invention

[0004] The main objective of this invention is to provide a temperature-controlled culture device and related equipment, which aims to improve the accuracy of culture experiments, make the environment simulation of the culture device more realistic, and greatly improve its applicability and convenience.

[0005] To achieve the above objectives, one aspect of the present invention provides a temperature-controlled culture device, comprising: a culture reactor, an intelligent controller, and a monitoring and execution component; The culture reactor is used to simulate the natural environment based on environmental parameters; The intelligent controller is fixed to any side of the culture reactor and is used to generate control signals for the culture reactor using a multi-level linkage PID algorithm based on the multi-dimensional environmental parameters output by the monitoring and execution component and the environmental parameters of the target area obtained through the network. The monitoring and execution component is assembled inside the culture reactor and includes a monitoring component and an execution component; The monitoring component, connected to the intelligent controller, includes a temperature sensor and a liquid level sensor, and is used to monitor multiple environmental parameters of the culture reactor based on the temperature sensor, the light sensing module, and the liquid level sensor, and to send the monitored multiple environmental parameters to the intelligent controller. The execution component is connected to the intelligent controller and is used to adjust the environmental parameters of the culture reactor according to the control signal output by the intelligent controller.

[0006] In some embodiments, the culture reactor includes: a culture shell, a culture tank, a culture vessel, a cap, a heating module, a cooling module, a sliding rod, a cable outlet, a lighting assembly, a raised platform, a water outlet, a shock-absorbing bracket, a vent, and a sample inlet / sampling port; The culture shell is located on the outer surface of the culture reactor and is provided with at least one heat dissipation hole to maintain the stable internal temperature of the culture reactor. The culture tank is located inside the culture shell. The culture tank is a circular box with an open top surface. Heating modules are symmetrically arranged in the upper and lower regions of the inner sidewall of the culture tank. A wire outlet and a lighting component are provided at the top. An opening is provided at the bottom of the culture tank. Cooling modules are arranged on both sides of the opening and are sealed and connected through the opening. The raised platform is located at the bottom center of the culture tank; The inlet and outlet are located at the bottom of the culture tank; The sliding rods are symmetrically installed on the side wall of the culture tank; The culture tank is suspended and fixed to the geometric center of the culture trough by a shock-absorbing bracket; The shock-absorbing bracket is fitted around the raised platform; The cap is threadedly connected to the opening of the culture tank and is pressed tightly against the top end face of the culture tank. It includes a vent with a microporous membrane and a sample inlet with a threaded sealing cap.

[0007] In some implementations, the intelligent controller includes: a main control circuit board, a touch screen, and a USB interface; The main control circuit board, connected to the touch screen and the USB interface, includes: a high-performance microprocessor, a network communication module, a light control module, a temperature control module, a data storage module, and a wiring module; The wiring module is communicatively connected to the monitoring and execution component and is used to transmit sensor data and control signals; The high-performance microprocessor is equipped with a multi-level linkage PID algorithm, which acquires real-time environmental data through a network communication module to analyze the changing trends, dynamically adjusts the working strategies of the temperature control component and the lighting component, and adaptively adjusts the electronically controlled valve and the circulating pump based on the detection data of the liquid level sensor. The touchscreen is used to set the environmental parameters of the culture reactor; The USB interface is used to transmit data and signals.

[0008] In some embodiments, the monitoring components include a level sensor and a temperature sensor; Both the liquid level sensor and the temperature sensor are fixed on the connecting sleeve; The wires of the liquid level sensor and the temperature sensor are both connected to the outlet along the extension direction of the sliding rod. The connecting sleeve is slidably fitted onto the sliding rod and locked in a preset position by a set screw; The actuators include a temperature control unit, a lighting unit, a circulating pump, and an electrically controlled valve; The outlet of the electrically controlled valve is sealed to the inlet and outlet of the water inlet; The circulating pump is fixed on a raised platform at the center of the bottom of the culture tank. The inlet of the circulating pump is parallel to the raised platform, and the outlet is perpendicular to the top. The temperature control component includes a heating module and a cooling module; The heating module is fixed inside the culture tank, and the cooling module is installed at the bottom of the culture tank and the bottom of the culture shell and is sealed and connected through an opening at the bottom of the culture tank. The lighting assembly includes a full-spectrum lamp and a lighting port; The full-spectrum lamp is spirally connected to the lighting port.

[0009] In some embodiments, the shock-absorbing bracket includes a barrier and a shock-absorbing rod; The barrier is disposed on the side of the upper plane of the shock-absorbing support and is used to fix the culture tank; The shock absorber is used to provide elastic support and reduce vibration.

[0010] In some embodiments, the electrically controlled valve is a three-way ball valve with two inlets and one outlet; The first inlet of the electrically controlled valve is connected to an external water source pipeline, and the second inlet is connected to a drainage pipeline. The valve's rotation is controlled by an electromagnetic controller.

[0011] In some embodiments, the temperature control component includes a heating module and a cooling module; The heating module includes a heating slot and a heating cartridge. The heating slot of the heating module has a rectangular opening and an elastic buckle on the inside. The heating cartridge is inserted into the heating slot from the side and is quickly fixed by the elastic buckle. The heating cartridge includes: a ceramic heating element and electrical contacts; The ceramic heating element is used for heating; The electrical contacts are used for signal transmission and power supply; The cooling module is used for refrigeration and includes a refrigeration compressor, a condenser, and a radiator. The refrigeration compressor and the radiator are installed at the bottom of the culture shell, and the condenser is installed at the bottom of the culture tank. The condenser is sealed to the refrigeration compressor and the radiator through an opening at the bottom of the culture tank.

[0012] To achieve the above objectives, another aspect of the present invention provides a method for using a temperature-controlled culture device, which is applied to a temperature-controlled culture device and includes: The initial environmental parameters within each culture reactor are set using an intelligent controller. Environmental data within the culture reactor is acquired using monitoring and execution components; An intelligent controller is used to acquire environmental parameters of the target area via a network; An intelligent controller is used to dynamically adjust the environmental parameters of the culture reactor based on the environmental parameters of the target area and the environmental data inside the culture reactor. The monitoring and execution components are used to acquire water level data in each culture reactor; An intelligent controller is used to adjust the direction of the electrically controlled valves based on the water level data and preset water level range in the culture reactor.

[0013] In some embodiments, the environmental parameters of the culture reactor include temperature and light parameters, and the use of an intelligent controller to dynamically adjust the environmental parameters of the culture reactor based on the environmental parameters of the target area and the environmental data within the culture reactor includes: Calculate the temperature data of the baseline environmental water / soil based on the environmental parameters of the target area; The temperature data of the reference environmental water / soil is expressed as follows:

[0014] in, The ambient surface temperature, The current value is the real-time weather temperature, C is the current cloud cover, P is the current precipitation, V is the current wind speed, and RH is the current relative humidity. K is atmospheric pressure, k1 is the dominant coefficient of temperature, k2 is the cloud cover-radiation correction coefficient, k3 is the precipitation cooling coefficient, k4 is the wind speed heat dissipation coefficient, k5 is the wind speed nonlinear exponent, k6 is the humidity evaporation correction coefficient, and k7 is the air pressure correction coefficient. The temperature parameters of the culture reactor are dynamically adjusted based on the pre-set target temperature difference and the temperature data of the reference environmental water / soil; the target temperature difference is the difference between the culture temperature data of the culture reactor and the temperature data of the reference environmental water / soil. The illumination parameters of the culture reactor are adjusted in conjunction with the illumination conditions of the target area and the temperature parameters.

[0015] In some embodiments, the step of using an intelligent controller to adjust the direction of the electrically controlled valve based on the water level data in the culture reactor and a preset water level range includes: Compare the water level data in the culture reactor with the preset water level; When the water level in the culture reactor is lower than the preset water level, the electronically controlled valve is switched to the first inlet to supply water. When the water level in the culture reactor is higher than the preset water level, the electronically controlled valve is switched to the second inlet to drain water. If the water level in the culture reactor exceeds the preset water level for more than a preset time, the intelligent controller will issue an audible and visual alert.

[0016] The beneficial effects of this invention's temperature-controlled culture device and related equipment are as follows: The culture device comprises three main modules: a culture reactor, an intelligent controller, and a monitoring and execution component. The culture reactor can intelligently simulate the natural environment based on control parameters, achieving high control precision and simulation similarity. The intelligent controller, equipped with a multi-level linkage PID algorithm and fixed to any side of the culture reactor, can manually or automatically adjust the environmental parameters of the culture reactor as needed, supporting dynamic adjustment and feedback control of the environmental parameters, ensuring that the culture reactor remains consistent with the natural environment. The monitoring and execution component can collect data from the culture reactor in real time and forward the collected data to the intelligent controller, better guiding the intelligent controller in environmental control of the culture reactor. This invention achieves a multi-level linkage dynamic temperature control mechanism, dynamically simulating the dynamic natural environment of temperature fluctuations under climate change, and supporting multi-unit parallel independent experiments, effectively improving the reliability and stability of the culture process. Furthermore, it can remotely acquire environmental parameters of any target area to provide data support for the generation of control signals, significantly improving applicability and convenience. Attached Figure Description

[0017] The accompanying drawings, which are incorporated in and form part of this specification, illustrate embodiments consistent with the invention and, together with the description, serve to explain the principles of the invention.

[0018] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, for those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0019] Figure 1 This is a schematic diagram of a temperature-controlled culture device provided in an embodiment of the present invention; Figure 2 This is a side cross-sectional view of the culture reactor provided in an embodiment of the present invention; Figure 3 This is a front cross-sectional view of the culture reactor provided in an embodiment of the present invention; Figure 4 This is a schematic diagram of the structure of the cap provided in an embodiment of the present invention; Figure 5 This is a schematic diagram of the structure of the heating module provided in an embodiment of the present invention; Figure 6 This is a schematic diagram of the cooling module provided in an embodiment of the present invention; Figure 7 This is a schematic diagram of the structure of the intelligent controller provided in an embodiment of the present invention; Figure 8 This is an explosion diagram of the culture reactor provided in an embodiment of the present invention; Figure 9 This is an exploded view of the culture tank and monitoring execution components provided in an embodiment of the present invention; Figure 10 This is a schematic diagram of the pipeline provided in an embodiment of the present invention.

[0020] Explanation of reference numerals in the attached drawings: 1. Culture reactor; 101. Culture shell; 102. Culture tank; 103. Culture vessel; 104. Raised platform; 105. Inlet / outlet; 106. Heat dissipation hole; 201. Sliding rod; 202. Connecting sleeve; 203. Liquid level sensor; 204. Temperature sensor; 301. Heating slot; 302. Heating cartridge; 303. Electrical contact point; 304. Ceramic heating element; 305. Refrigeration compressor; 306. Condenser; 307. Radiator; 4. Vibration damping bracket; 401. Barrier; 402. Vibration damping rod; 5. Circulation pump; 501. Pump outlet; 502. Pump inlet; 6. Cover; 601. Inlet 602. Sampling port; 603. Threaded sealing cap; 604. Microporous membrane; 605. Vent hole; 7. Outlet port; 8. Electrically controlled valve; 806. First inlet; 807. Electromagnetic controller; 808. Second inlet; 9. Lighting assembly; 908. Full-spectrum lamp; 909. Lighting port; 10. Intelligent controller; 1000. Main control circuit board; 1001. High-performance microprocessor; 1002. Network communication module; 1003. Lighting control module; 1004. Temperature control module; 1005. Data storage module; 1006. Wiring module; 1007. Touch screen; 1008. USB interface; 1009. Water inlet pipe; 1000. Water outlet pipe. Detailed Implementation

[0021] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only a part of the embodiments of the present invention, and not all of them. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0022] It should be noted that all directional indications (such as up, down, left, right, front, back, etc.) in the embodiments of the present invention are only used to explain the relative positional relationship and movement of each component in a certain specific posture (as shown in the figure). If the specific posture changes, the directional indication will also change accordingly.

[0023] Furthermore, the use of terms such as "first" and "second" in this invention is for descriptive purposes only and should not be construed as indicating or implying their relative importance or implicitly specifying the number of technical features indicated. Therefore, features defined with "first" and "second" may explicitly or implicitly include at least one of those features. Additionally, the technical solutions of the various embodiments can be combined with each other, but only on the basis of being achievable by those skilled in the art. When the combination of technical solutions is contradictory or impossible to implement, such a combination of technical solutions should be considered non-existent and not within the scope of protection claimed by this invention.

[0024] In the field of ecological and environmental science research, cultivation devices are the core equipment for simulating natural ecosystems and studying the growth patterns of organisms and the correlation between environmental factors.

[0025] Existing culture equipment has low temperature control precision and cannot make corresponding changes according to changes in the external environment. This makes it difficult to simulate the dynamic changes in the natural environment with the external temperature. Relying on constant temperature experiments, it is impossible to simulate the actual temperature fluctuation characteristics in the natural environment. This leads to deviations between environmental research results and actual conditions, and the simulation experiments are inaccurate. At the same time, the simulation experiments are easily limited by the differences in natural environmental temperature factors in different geographical regions, resulting in poor regional applicability.

[0026] Based on this, the main objective of this invention is to provide a temperature-controlled culture device, which aims to improve the accuracy of culture experiments, make the environment simulation more realistic, and provide data support for the generation of control signals by remotely acquiring environmental parameters of any target area, thereby greatly improving applicability and convenience.

[0027] This invention provides a temperature-controlled culture device and related equipment. Multiple culture tanks in the culture reactor of this invention can conduct experiments in parallel and independently, effectively improving the reliability and stability of the culture process, and can be widely used in ecological simulation and scientific research. The following embodiments illustrate this invention, starting with a temperature-controlled culture device.

[0028] Figure 1 This is a schematic diagram of a temperature-controlled culture device provided in an embodiment of the present invention. Please refer to [the diagram]. Figure 1 The temperature-controlled culture device provided in this embodiment of the invention may include, but is not limited to, a culture reactor 1, an intelligent controller 10, and a monitoring and execution component.

[0029] The culture reactor 1 is used to simulate the natural environment based on environmental parameters.

[0030] Optionally, the culture reactor 1 can adjust the temperature and light intensity inside the culture reactor 1 based on preset or real-time collected environmental parameters via an intelligent controller 10. Furthermore, a heat insulation layer is provided between each culture reactor 1 to ensure that the environment of each culture reactor 1 does not affect each other. The culture reactor 1 can accurately simulate the temperature gradient and light rhythm of the target natural environment, making the environmental conditions inside the reactor highly consistent with the natural ecological scene. This provides growth conditions consistent with the natural environment for the culture of various biological samples, cell tissues, or microbial communities, ensuring that the physiological state and metabolic processes of the cultured objects remain consistent with their performance under natural conditions.

[0031] The intelligent controller 10 is fixed to any side of the culture reactor 1 and is used to generate control signals for the culture reactor based on the multi-dimensional environmental parameters output by the monitoring and execution component and the environmental parameters of the target area obtained through the network using a multi-level linkage PID algorithm. In some embodiments, the multi-level linkage PID algorithm is an intelligent control algorithm based on the classic proportional-integral-derivative (PID) control logic, adopting a hierarchical and parameter linkage architecture. It is suitable for complex control systems with multi-variable coupling and multi-execution unit collaboration. The intelligent controller 10 includes a network communication module 1003, which can acquire environmental parameters of the target area through the network communication module 1003. As an optional implementation, the intelligent controller 10 can receive in real time multiple environmental parameters such as temperature and light intensity output by the monitoring and execution components of the culture reactor 1, as well as outdoor environmental parameters acquired through the network communication module 1003. It performs coupled analysis and deviation calculation on these two types of parameters using a multi-level linkage PID algorithm, generating a suitable and precise control signal and sending it to the temperature control and light control execution units of the culture reactor 1. This dynamically adjusts various environmental parameters inside the reactor, achieving real-time matching and stable maintenance of the reactor environment with the target simulation scenario. In this invention, the network communication module 1003 of the intelligent controller can independently access environmental data from multiple regions, such as obtaining local weather forecasts from meteorological websites in the target area. This data has high reliability and can simulate a wider range of areas. Therefore, the temperature-controlled culture device provided by this invention can remotely acquire environmental parameters from any target area to provide data support for the generation of control signals, significantly improving its applicability and convenience.

[0032] The monitoring and execution component is assembled inside the culture reactor 1 and includes a monitoring component and an execution component; The monitoring component, connected to the intelligent controller 10, includes a temperature sensor, a light sensor, and a liquid level sensor; it is used to monitor multiple environmental parameters of the culture reactor 1 based on the temperature sensor, light sensor, and liquid level sensor, and to send the monitored multiple environmental parameters to the intelligent controller 10. The execution component is connected to the intelligent controller 10 and is used to adjust the environmental parameters of the culture reactor 1 using the control signals output by the intelligent controller 10.

[0033] It is easy to understand that the monitoring and execution components are divided into two parts. The first part is the monitoring component, which integrates various types of sensors and is mainly used to acquire environmental data inside the culture reactor 1. The second part is the execution component, which is mainly used to directly regulate the environmental parameters of the culture reactor 1. The adjustable environmental parameters include temperature, light, and water level.

[0034] Further, refer to Figure 1 , Figure 2 , Figure 3 and Figure 8 The culture reactor 1 includes: a culture shell 101, a culture tank 102, a culture vessel 103, a cover 6, a heating module, a cooling module, a sliding rod 201, a cable outlet 7, a lighting assembly 9, a raised platform 104, a water outlet, a shock-absorbing bracket 4, a vent 604, and a sample inlet 601. The culture shell 101 is located on the outer surface of the culture reactor 1 and is provided with at least one heat dissipation hole 106 to maintain the internal temperature of the culture reactor 1. The culture tank 102 is located inside the culture shell 101. The culture tank 102 is a circular box with an open top surface. Heating modules are symmetrically arranged in the upper and lower regions of the inner sidewall of the culture tank 102. The top is provided with a cable outlet 7 and a lighting component 9. An opening is provided at the bottom of the culture tank 102. The cooling modules are arranged on both sides of the opening and are sealed and connected through the opening. The raised platform 104 is located at the bottom center of the culture tank 102; The inlet / outlet 105 is located at the bottom of the culture tank 102; The sliding rod 201 is symmetrically installed on the side wall of the culture tank 102; The culture tank 103 is suspended and fixed to the geometric center of the culture trough 102 by a shock-absorbing bracket 4; The shock-absorbing bracket 4 is fitted around the raised platform 104; The cap 6 is threadedly connected to the opening of the culture tank 103 and is pressed and sealed to the top end face of the culture tank 102. It includes a vent 604 with a microporous membrane 603 and a sample inlet 601 with a threaded sealing cap 602.

[0035] Among them, reference Figure 4 The cap 6 is made of an opaque light-blocking material, which can effectively block external light interference. The microporous membrane 603 is fixed to the surface of the vent 604. The sample inlet 601 is located in the area of ​​the corresponding culture tank 103 and is sealed by the threaded sealing cap 602.

[0036] In some embodiments, the culture reactor 1 includes a culture shell 101, a culture tank 102, a culture vessel 103, and a sealing cap 6 arranged sequentially from the outside to the inside. The culture shell 101 is provided with at least one heat dissipation hole 106 to discharge heat outwards and maintain a stable internal temperature. The culture tank 102 is located inside the culture shell 101 and is a circular box with an open top. Heating modules are symmetrically arranged in the upper and lower regions of its inner wall. Sliding rods 201 are symmetrically installed along the vertical direction. An outlet 7 and a lighting assembly 9 are provided at the top. A raised platform 104 is located at the center of the bottom, and an inlet / outlet 1 is provided on one side of the bottom. 05. Furthermore, the bottom of the culture tank 102 is provided with an opening, and the cooling module is arranged on both sides of the opening and connected by a sealing connection through the opening, thereby realizing the transfer of heat inside the culture tank 102 to the external environment through the cooling module to achieve heat dissipation and cooling; the culture vessel 103 is suspended and fixed to the geometric center of the culture tank 102 by a shock-absorbing bracket 4, the shock-absorbing bracket 4 is fitted around the raised platform 104, and the cover 6 integrates a vent 604 with a microporous membrane 603 and a sample inlet 601 with a threaded seal. The cover 6 is threadedly sealed to the opening of the culture vessel 103 and is also pressed and sealed to the top end face of the culture vessel.

[0037] Further, refer to Figure 7 The intelligent controller 10 includes: a main control circuit board 1001, a touch screen 1008, and a USB interface 1009; The main control circuit board 1001 is connected to the touch screen 1008 and the USB interface 1009, and includes: a high-performance microprocessor 1002, a network communication module 1003, a light control module 1004, a temperature control module 1005, a data storage module 1006, and a wiring module 1007. The wiring module 1007 is communicatively connected to the monitoring and execution component and is used to transmit sensor data and control signals. The high-performance microprocessor 1002 is equipped with a multi-level linkage PID algorithm, which acquires real-time environmental data and analyzes the changing trends through the network communication module 1003, dynamically adjusts the working strategies of the temperature control component and the lighting component 9, and adaptively adjusts the electronically controlled valve 8 and the circulating pump 5 based on the detection data of the liquid level sensor 203.

[0038] The multi-level linkage PID algorithm uses real-time environmental data as a feedforward input, integrates it with the feedback data from the temperature sensor 204 and the target temperature difference, dynamically generates coordinated control commands for the temperature control component, and adjusts the operating parameters of the lighting component 9 in conjunction with the real-time solar intensity and the light requirements of the cultured objects in the culture reactor 1.

[0039] The touchscreen 1008 is used to set the environmental parameters of the culture reactor 1.

[0040] The touchscreen 1008 can display the temperature, water level, and light data of each culture unit in real time, and show dynamic curves.

[0041] The USB interface 1009 is used for transmitting data and signals.

[0042] In some embodiments, the intelligent controller 10, fixed to the side of the culture reactor 1, includes a main control circuit board 1001, a touch screen 1008, and a USB interface 1009. The main control circuit board 1001 integrates a high-performance microprocessor 1002, a network communication module 1003, a light control module 1004, a temperature control module 1005, a data storage module 1006, and a wiring module 1007, and is connected to the touch screen 1008 and the USB interface 1009 embedded on the front of the intelligent controller 10. The wiring module 1007 is communicatively connected to the monitoring and execution component for transmitting sensor data and control signals. The high-performance microprocessor 1002 is configured to run a multi-level linkage PID algorithm, obtain real-time environmental data analysis and trend changes through the network communication module 1003, dynamically adjust the working strategies of the temperature control component and the lighting component 9, and adaptively adjust the electrically controlled valve 8 and the circulating pump 5 based on the detection data of the liquid level sensor 203.

[0043] Further, refer to Figure 9 Monitoring components, including a level sensor 203 and a temperature sensor 204; Both the liquid level sensor 203 and the temperature sensor 204 are fixed on the connecting sleeve; The wires of the liquid level sensor 203 and the temperature sensor 204 are both connected to the outlet 7 along the extension direction of the sliding rod 201.

[0044] The temperature sensor 204 of the monitoring component is divided into an upper sensor and a lower sensor, which respectively monitor the temperature of the upper and lower regions of the culture tank 102. It can be slidably connected to the same vertical sliding rod 201 on the inner side wall of the culture tank 102 through an independent connecting sleeve.

[0045] The connecting sleeve is slidably fitted onto the sliding rod 201 and locked in a preset position by a set screw; The execution components include a temperature control component, a lighting component 9, a circulating pump 5, and an electrically controlled valve 8; The outlet of the electrically controlled valve 8 is sealed to the inlet / outlet 105; The circulation pump 5 is fixed on the raised platform 104 at the center of the bottom of the culture tank 102. The inlet of the circulation pump 5 is parallel to the raised platform 104, and the outlet is vertically facing the top.

[0046] When the temperature difference between the upper and lower sensors of the temperature sensor 204 exceeds a preset threshold, the intelligent controller 10 controls the circulating pump 5 to start continuous operation or increase its power.

[0047] The temperature control component includes a heating module and a cooling module; refer to Figure 2 and Figure 3 The heating module is fixed inside the culture tank 102, and the cooling module is installed at the bottom of the culture tank 102 and the bottom of the culture shell 101 and is sealed and connected through an opening at the bottom of the culture tank 102. The lighting component 9 includes a full-spectrum lamp 901 and a lighting port 902.

[0048] The emission spectrum, light intensity, and photoperiod of the full-spectrum lamp 901 are controlled by the high-performance microprocessor 1002 of the intelligent controller 10. The natural day and night light environment is simulated based on the acquired external environmental data of the target area. c. This application does not impose specific limitations on this.

[0049] The full-spectrum lamp 901 is spirally connected to the lighting port 902.

[0050] In some embodiments, a monitoring and execution component is assembled within the culture tank 102 of the culture reactor 1, comprising a monitoring component and an execution component; wherein, the monitoring component includes a level sensor 203 and a temperature sensor 204, respectively fixed to a connecting sleeve, the connecting sleeve being slidably fitted onto the sliding rod 201 and locked in a set position by a set screw; the wires of the level sensor 203 and the temperature sensor 204 are connected to the outlet 7 along the extension direction of the sliding rod 201; the execution unit includes an electrically controlled valve 8, the outlet end of which is connected to the outlet... The inlet 105 is sealed; the temperature control assembly includes a heating module and a cooling module. The heating module is fixed inside the culture tank 102 for heating, and the cooling module is installed at the bottom of the culture tank 102 and the culture shell 101 for cooling; the circulation pump 5 is fixed on the raised platform 104 at the center of the bottom of the culture tank 102. The inlet of the circulation pump 5 is parallel to the raised platform 104, and its outlet is vertically facing the top; the lighting assembly 9 includes a full-spectrum lamp 901 and an lighting port 902. The full-spectrum lamp 901 is spirally connected to the lighting port 902.

[0051] Furthermore, the shock absorber bracket 4 includes a barrier 401 and a shock absorber rod 402; The barrier 401 is disposed on the side of the upper plane of the shock-absorbing bracket 4 and is used to fix the culture tank 103; The shock absorber 402 is used to provide elastic support and reduce vibration.

[0052] In some embodiments, the shock-absorbing bracket 4 includes a barrier 401 and a shock-absorbing rod 402, wherein the barrier 401 is disposed on the side of the upper plane of the shock-absorbing bracket 4 for fixing the culture tank 103; the shock-absorbing rod 402 provides elastic support and reduces vibration.

[0053] Further, refer to Figure 10 The electrically controlled valve 8 is a three-way ball valve with two inlets and one outlet. The first inlet 801 of the electrically controlled valve 8 is connected to an external water source pipeline, and the second inlet 803 is connected to a drainage pipeline. The direction of rotation of the electrically controlled valve 8 is controlled by an electromagnetic controller 802.

[0054] In some embodiments, the electrically controlled valve 8 adopts a two-inlet-one-outlet three-way ball valve structure, wherein the first inlet 801 is connected to an external water source pipeline as an inlet pipeline 11, the second inlet 803 is connected to a drainage pipeline as an outlet pipeline 12, and the electromagnetic controller 802 controls the valve's rotation direction.

[0055] Further, refer to Figure 5 and Figure 6 The temperature control component includes a heating module and a cooling module; The heating module includes a heating slot 301 and a heating cartridge 302. The heating slot 301 of the heating module has a rectangular opening and an elastic buckle on the inside. The heating cartridge 302 is inserted into the heating slot 301 from the side and is quickly fixed by the elastic buckle. The heating cartridge 302 includes: a ceramic heating element 304 and an electrical contact 303; The ceramic heating element 304 is used for heating; The electrical contact 303 is used for signal transmission and power supply; The cooling module is used for refrigeration and includes a refrigeration compressor 305, a condenser 306, and a radiator 307. The refrigeration compressor 305 and the radiator 307 are installed at the bottom of the culture shell 101, and the condenser 306 is installed at the bottom of the culture tank 102. The condenser 306 is sealed to the refrigeration compressor 305 and the radiator 307 through an opening at the bottom of the culture tank 102.

[0056] In some embodiments, the heating slot 301 of the heating module has a rectangular opening and an elastic buckle on the inside; the heating cartridge 302 of the heating module includes a ceramic heating element 304 and an electrical contact 303, the ceramic heating element 304 provides heating; the electrical contact 303 is used for signal transmission and power supply of the heating slot 301 and the heating cartridge 302; the cooling module is used for cooling and includes a cooling compressor 305, a condenser 306 and a radiator 307, the cooling compressor 305, the condenser 306 and the radiator 307 form a cooling cycle loop to dissipate heat from the culture tank 102 to reach and maintain the required temperature inside the culture tank.

[0057] As a further optional implementation, a temperature control strategy is incorporated into the temperature control component, wherein the temperature control strategy includes: activating the heating module when the real-time temperature difference ΔT' is lower than the set temperature difference ΔT; activating the cooling module when ΔT' is higher than ΔT; and activating the cooling module according to |ΔT' The heating / cooling power is dynamically adjusted by ΔT∣ (the absolute value of the temperature difference between the real-time temperature difference and the set temperature difference); the circulating pump 5 always keeps running, and adopts intermittent mode during the heat preservation stage (ΔT'=ΔT) to maintain the temperature fluctuation range in the culture tank 102 within ≤±0.1℃. If the abnormal state continues for more than a certain period of time, the intelligent controller 10 will issue an audible and visual prompt.

[0058] The following section provides a detailed introduction and explanation of the scheme of this invention embodiment, using specific simulated culture scenarios: A temperature-controlled culture device includes: a culture reactor 1, comprising a culture shell 101, a culture tank 102, a culture vessel 103, and a sealing cap 6 arranged from the outside to the inside; wherein, the culture shell 101 is provided with at least one heat dissipation hole 106 for maintaining internal temperature stability; the culture tank 102 is placed inside the culture shell 101 and is a circular box with an open top surface, with heating modules symmetrically arranged in the upper and lower regions of its inner sidewalls, and sliding rods 201 symmetrically installed along the vertical direction, and a wire outlet 7 and a lighting component 9 at the top; the bottom of the culture tank 102 is provided with... There is an opening, and cooling modules are arranged on both sides of the opening and connected by a sealing connection through the opening. There is a raised platform 104 at the center of the bottom, and an inlet / outlet 105 is provided on one side of the bottom. The culture tank 103 is suspended and fixed to the geometric center of the culture tank by a shock-absorbing bracket 4. The shock-absorbing bracket 4 is fitted around the raised platform 104. The cover 6 integrates a vent 604 with a microporous membrane 603 and a sample inlet 601 with a threaded sealing cover 602. The cover 6 is threadedly sealed to the opening of the culture tank 103 and is also pressed and sealed to the top end face of the culture tank 102.

[0059] A monitoring and execution component, assembled within the culture tank 102 of the culture reactor 1, includes a monitoring component and an execution component. The monitoring component includes a level sensor 203 and a temperature sensor 204, respectively fixed to a connecting sleeve 202. The connecting sleeve 202 is slidably fitted onto a sliding rod 201 and locked in a set position by a set screw. The wires of the level sensor 203 and the temperature sensor 204 extend along the sliding rod 201 and are connected to the outlet 7. The execution unit includes an electrically controlled valve 8, whose outlet end is sealed to the inlet / outlet water port 105. The temperature control component includes... A heating module and a cooling module are provided. The heating module is fixed inside the culture tank 102, and the cooling module is installed at the bottom of the culture tank 102 and connected to the bottom of the culture shell 101 through an opening at the bottom of the culture tank 102. A circulation pump 5 is fixed on a raised platform 104 at the center of the bottom of the culture tank 102. The pump inlet 502 of the circulation pump 5 is parallel to the raised platform 104, and its pump outlet 501 faces vertically to the top. The lighting assembly 9 includes a full-spectrum lamp 901 and an lighting port 902. The full-spectrum lamp 901 is spirally connected to the lighting port 902.

[0060] The intelligent controller 10, fixed to the side of the culture reactor 1, includes a main control circuit board 1001, a touch screen 1008, and a USB interface 1009. The main control circuit board 1001 integrates a high-performance microprocessor 1002, a network communication module 1003, a light control module 1004, a temperature control module 1005, a data storage module 1006, and a wiring module 1007, and is connected to the touch screen 1008 and USB interface 1009 embedded on the front of the intelligent controller 10. The wiring module 1007 is communicatively connected to the monitoring and execution components for transmitting sensor data and control signals. The high-performance microprocessor 1002 is configured to run a multi-level linkage PID algorithm, obtain real-time environmental data through the network communication module 1003 to analyze the changing trends, dynamically adjust the working strategies of the temperature control components and the lighting components 9, and adaptively adjust the electrically controlled valve 8 and the circulating pump 5 based on the detection data of the liquid level sensor 203.

[0061] The above-mentioned temperature-controlled culture device was used to conduct culture experiments, including: The microalgae culture experiment consists of the following steps: Device Assembly: In each culture tank, place the culture vessel on the shock-absorbing support, maintaining a gap between the outer wall and the tank wall. Fix the upper and lower sensors to the connecting sleeves respectively, adjust them to the required height along the sliding rod, tighten the set screw, adjust the height of the liquid level sensor according to the required water level, insert the heating cartridge into the heating slot and confirm locking, and connect the intelligent controller to each component of the temperature-controlled culture device via the communication line.

[0062] Ecosystem construction: Inoculate each culture tank with algae and add culture medium to the same height as the liquid level sensor, and seal the tank with a cap.

[0063] Parameter settings: Select "Aquatic Ecosystem" and the target area on the touchscreen, and set the preset water level. The microprocessor accesses a meteorological website via the network to obtain the ambient temperature and sunrise / sunset times of the target area, calculates the temperature of the dynamic environmental water body, and each unit determines the cultivation temperature based on the temperature difference and the ambient water temperature. It also determines the light intensity and photoperiod based on the environmental data.

[0064] Cultivation process: The intelligent controller drives the temperature control component and the light component through the PID algorithm to maintain a stable temperature in the cultivation tank, with temperature fluctuations kept within ±0.1℃; the water level is maintained by the liquid level sensor.

[0065] The lake microcosm experiment consists of the following steps: Device Assembly: In each culture tank, place the culture vessel on the shock-absorbing support, maintaining a gap between the outer wall and the tank wall. Fix the upper and lower sensors to the connecting sleeves respectively, adjust them to the required height along the sliding rod, tighten the set screw, adjust the height of the liquid level sensor according to the required water level, insert the heating cartridge into the heating slot and confirm locking, and connect the intelligent controller to each component of the temperature-controlled culture device via the communication line.

[0066] Ecosystem construction: Raw lake water and bottom sediment were injected into the culture tank, and phytoplankton and animals were inoculated at the same height as the liquid level sensor. A 0.22μm microporous membrane was used for sealing to prevent external microbial contamination.

[0067] Parameter settings: Select "Aquatic Ecosystem" and the target area on the touchscreen, and set the preset water level. The microprocessor obtains the ambient temperature and sunrise / sunset time of the target area by remotely accessing a meteorological website, calculates the temperature of the dynamic environmental water body, and each unit determines the cultivation temperature based on the temperature difference and the ambient water temperature, and determines the light intensity and photoperiod through environmental data.

[0068] Cultivation process: The intelligent controller drives the temperature control component and the light component through the PID algorithm to maintain a stable temperature in the cultivation tank, with temperature fluctuations kept within ±0.1℃; the water level is maintained by the liquid level sensor.

[0069] The forest soil incubation experiment consists of the following steps: Device Assembly: In each culture tank, suspend the culture vessel from the shock-absorbing bracket, maintaining a gap between the outer wall and the tank wall. Fix the upper and lower sensors to the connecting sleeves respectively, adjust them to the required height along the sliding rod, tighten the set screw, adjust the height of the liquid level sensor between the temperature sensor and the liquid level sensor, insert the heating cartridge into the heating slot and confirm locking, and connect the intelligent controller to each component via the communication line.

[0070] Ecosystem construction: Forest soil was collected and placed in a culture tank at the same level as the liquid level sensor, and the cap was sealed to the culture unit.

[0071] Parameter settings: Select "Soil Ecosystem" and target area on the touchscreen, and set the preset water level. The microprocessor obtains the ambient temperature and sunrise / sunset time of the target area by remotely accessing a meteorological website, calculates the dynamic environmental soil temperature, and each unit determines the cultivation temperature based on the temperature difference and environmental soil temperature. It also determines the light intensity and photoperiod based on environmental data.

[0072] Cultivation process: The intelligent controller drives the temperature control component and the light component through the PID algorithm to maintain a stable temperature in the cultivation tank, with temperature fluctuations kept within ±0.1℃; the water level is maintained by the liquid level sensor.

[0073] The above embodiments are merely illustrative of the technical concept and features of the present invention and do not limit the scope of protection of the present invention. All equivalent changes and modifications made within the scope of the claims of the present invention should fall within the coverage of the claims of the present invention.

[0074] This invention also provides a method for using a temperature-controlled culture device, which is applied to a temperature-controlled culture device. The method for using the temperature-controlled culture device includes: Step S100: Use an intelligent controller to set the initial environmental parameters in each culture reactor.

[0075] Optionally, when setting the initial environmental parameters for each culture reactor using an intelligent controller, this can be done via a touchscreen. Furthermore, a pre-set culture process parameter library is imported through the controller's human-machine interface. For different culture objects in different reactors, corresponding threshold values ​​for core parameters such as temperature, light intensity, and water level are set. Subsequently, the controller uses a multi-sensor calibration module to calibrate the zero point and range of the sensing units in each reactor, ensuring parameter acquisition accuracy. Then, through an internal PID control algorithm combined with a fuzzy control strategy, precise drive commands are sent to the actuators of each reactor, completing the parameter initialization step by step. Simultaneously, the controller collects parameter feedback data from each reactor in real time, compares it with the set values, and dynamically fine-tunes until the initial environmental parameters of all reactors are stable within the required process range.

[0076] Step S200: Use the monitoring execution component to acquire environmental data inside the culture reactor.

[0077] It is easy to understand that the intelligent controller can receive various environmental parameters such as temperature and light intensity from the monitoring and execution components of the culture reactor in real time.

[0078] Step S300: Use an intelligent controller to obtain environmental parameters of the target area via the network.

[0079] In some embodiments, the intelligent controller can access the meteorological website of the target area via the network and obtain the environmental parameters from the meteorological website. Therefore, the present invention can simulate the climate conditions of various regions without distance limitations, and the use of meteorological website data is more reliable, making the simulation of the cultivation reactor closer to the natural environment and more accurate.

[0080] Step S400: The intelligent controller dynamically adjusts the environmental parameters of the culture reactor based on the environmental parameters of the target area and the environmental data inside the culture reactor.

[0081] For example, when an intelligent controller dynamically adjusts temperature and light parameters based on real-time external environmental data, feedback data from temperature sensors, and the target temperature difference, the controller first synchronously receives real-time data such as ambient temperature and light intensity transmitted by the monitoring and execution components through the communication interface, as well as precise feedback values ​​from the temperature sensors in each culture reactor. Then, it calculates the difference between the actual temperature inside the reactor and the preset target temperature to obtain the real-time temperature difference. This difference is then combined with external environmental data to establish a multi-parameter coupled control model. If the real-time temperature difference exceeds the allowable fluctuation range, the controller will send graded drive commands to the temperature regulation execution mechanism based on the built-in fuzzy PID algorithm, reducing the temperature difference by increasing or decreasing heating power or adjusting the cooling rate. Simultaneously, based on the light intensity of the target area and the light requirements of the cultured objects in the reactor, it will coordinately adjust the brightness and irradiation duration of the light execution components. Throughout the entire control process, the controller continuously collects temperature and light feedback data, compares it with the target values ​​in real time, and repeatedly fine-tunes it to ensure that the temperature and light parameters inside the reactor remain stable within the optimal range, guaranteeing the efficient progress of the culture process.

[0082] Step S500: The monitoring execution component is used to acquire water level data in each culture reactor.

[0083] In some embodiments, when the monitoring and execution component acquires water level data in each culture reactor, the component deploys a corresponding water level detection unit (such as an ultrasonic level sensor, a capacitive level sensor, or a float level sensor) for each reactor. The component performs fixed-point or continuous detection of the culture medium level in the reactor according to a preset acquisition cycle, and then transmits the real-time water level data of each reactor to the intelligent controller through a data transmission channel. At the same time, the component temporarily stores historical water level data in a local cache. When the water level is detected to exceed a preset threshold, a local warning is immediately triggered and an alarm message is sent to the controller, providing accurate data support for subsequent automatic replenishment or discharge control of the liquid level.

[0084] In step S600, an intelligent controller adjusts the direction of the electrically controlled valve based on the water level data in the culture reactor and the preset water level range.

[0085] Optionally, when the intelligent controller adjusts the direction of the electrically controlled valves based on the water level data in the culture reactor and the preset water level range, it receives the water level data of each reactor transmitted by the monitoring and execution components in real time, and compares it with the preset upper limit, lower limit and optimal range of the water level one by one. If the water level of a certain reactor is detected to be lower than the preset lower limit, the controller will immediately send a positive drive command to the corresponding inlet electrically controlled valve to control the valve to open and adjust the opening degree to replenish the culture medium; if the water level is higher than the preset upper limit, it will send a reverse drive command to the drain electrically controlled valve to control the valve to open to discharge the excess culture medium; if the water level is in the optimal range, it will send a closing command to the valve to maintain the current liquid level. Throughout the entire control process, the controller will continuously collect water level feedback data and dynamically fine-tune the valve opening degree, ultimately achieving precise and automatic control of the water level of each reactor.

[0086] Furthermore, step S300 may include, but is not limited to, steps S310 to S330.

[0087] Step S310: Calculate the temperature data of the baseline environmental water / soil based on the environmental parameters of the target area; the environmental parameters of the culture reactor include temperature parameters and light parameters; The temperature data of the reference environmental water / soil is expressed as follows:

[0088] in, The ambient surface temperature, The real-time weather temperature is represented by C, the current cloud cover by P, the current precipitation by V, the current wind speed by RH, and the current relative humidity by P. atm K is atmospheric pressure, k1 is the dominant coefficient of temperature, k2 is the cloud cover-radiation correction coefficient, k3 is the precipitation cooling coefficient, k4 is the wind speed heat dissipation coefficient, k5 is the wind speed nonlinear exponent, k6 is the humidity evaporation correction coefficient, and k7 is the air pressure correction coefficient.

[0089] Optionally, in multiple culture reactors of a temperature-controlled culture device, the temperature data of the reference environmental water / soil can be the temperature data of the control group water / soil. The culture reactors are temperature-controlled by means of water baths or the like. Different culture reactors can also be compared with each other. The culture temperature data of the culture reactors other than the control culture reactor are adjusted according to the temperature data of the reference environmental water / soil.

[0090] Step S320: Dynamically adjust the temperature parameters of the culture reactor based on the pre-set target temperature difference and the temperature data of the reference environmental water / soil.

[0091] In some embodiments, based on the target temperature difference preset in the current culture reactor, the target temperature difference is added to or subtracted from the temperature data of the reference environmental water / soil to obtain the environmental parameters of the current culture reactor, and the heating module or cooling module is activated to adjust the temperature of the current culture reactor.

[0092] The target temperature difference, which is the difference between the culture temperature data of the culture reactor and the temperature data of the reference environmental water / soil, reflects the temperature difference between the current culture reactor and the control culture reactor. For example, the culture temperature data of the first culture reactor differs from the temperature data of the reference environmental water / soil of the control culture reactor by 2°C. The target temperature difference can be freely set according to experimental requirements.

[0093] Step S330: Adjust the light parameters of the culture reactor in conjunction with the light conditions of the target area and the temperature parameters.

[0094] Furthermore, the working strategy of the lighting components can be dynamically adjusted by combining environmental data of the target area (such as lighting conditions) and temperature parameters controlled based on the target temperature difference preset in the current culture reactor, thereby achieving dynamic control of the temperature and lighting parameters of the culture reactor.

[0095] Furthermore, step S600 may include, but is not limited to, steps S610, S620A and S630 or steps S610, S620B and S630.

[0096] Step S610: Compare the water level data in the culture reactor with the preset water level; Step S620A: When the water level in the culture reactor is lower than the preset water level, the electronically controlled valve is switched to the first inlet to supply water. Step S620B: When the water level in the culture reactor is greater than the preset water level, control the electronically controlled valve to turn to the second inlet to drain water; Step S630: If the water level in the culture reactor is higher than the preset water level for more than a preset time, the intelligent controller will issue an audible and visual alert.

[0097] In some embodiments, the intelligent controller compares the real-time water level data collected by the liquid level sensor with a preset range. When the water level is lower than the water level range, it controls the electrically controlled valve to switch to the first inlet to supply water; when the water level is higher than the water level range, it controls the electrically controlled valve to switch to the second inlet to drain water. If the abnormal state continues for more than a certain period of time, the intelligent controller will issue an audible and visual prompt. The intelligent controller can also have a built-in data storage module to record data at certain time intervals and supports exporting historical data via a USB interface.

[0098] This invention can dynamically simulate the natural environment with high precision. By introducing external environmental data and combining it with user settings, it calculates the dynamic target temperature, achieving accurate simulation of temperature changes in the natural environment and overcoming the limitations of traditional fixed-point temperature control. It features multi-factor intelligent linkage: through an intelligent central control module, it achieves closed-loop control and coordinated linkage of multiple environmental factors such as temperature, light, and water level, more realistically simulating the complex behavior of ecosystems. It is modular and easy to maintain: the heating cartridge adopts a quick-plug design, the sensor uses a sliding sleeve, and the lighting component is plug-and-play, greatly facilitating equipment maintenance, upgrades, and cleaning, and improving ease of use. It has wide scene adaptability: supporting simulation of various ecosystems such as lakes, forests, and grasslands, and allowing for parameter customization via a touchscreen, it is suitable for diverse experimental scenarios such as microbial culture and plant growth observation.

[0099] The device embodiments described above are merely illustrative. The units described as separate components may or may not be physically separate; that is, they may be located in one place or distributed across multiple network units. Some or all of the modules can be selected to achieve the purpose of this embodiment according to actual needs.

Claims

1. A temperature-controlled incubation device, characterized in that, include: Culture reactor, intelligent controller, and monitoring and execution components; The culture reactor is used to simulate the natural environment based on environmental parameters; The intelligent controller is fixed to any side of the culture reactor and is used to generate control signals for the culture reactor using a multi-level linkage PID algorithm based on the multi-dimensional environmental parameters output by the monitoring and execution component and the environmental parameters of the target area obtained through the network. The monitoring and execution component is assembled inside the culture reactor and includes a monitoring component and an execution component; The monitoring component, connected to the intelligent controller, includes a temperature sensor and a liquid level sensor, and is used to monitor multiple environmental parameters of the culture reactor based on the temperature sensor and the liquid level sensor, and send the monitored multiple environmental parameters to the intelligent controller. The execution component is connected to the intelligent controller and is used to adjust the environmental parameters of the culture reactor according to the control signal output by the intelligent controller.

2. The temperature-controlled incubation device according to claim 1, characterized in that, The culture reactor includes: a culture shell, a culture tank, a culture vessel, a cap, a heating module, a cooling module, a sliding rod, a cable outlet, a lighting assembly, a raised platform, inlet and outlet water inlets, a shock-absorbing bracket, a vent, and a sample inlet / outlet. The culture shell is located on the outer surface of the culture reactor and is provided with at least one heat dissipation hole to maintain the stable internal temperature of the culture reactor. The culture tank is located inside the culture shell. The culture tank is a circular box with an open top surface. Heating modules are symmetrically arranged in the upper and lower regions of the inner sidewall of the culture tank. A wire outlet and a lighting component are provided at the top. An opening is provided at the bottom of the culture tank. Cooling modules are arranged on both sides of the opening and are sealed and connected through the opening. The raised platform is located at the bottom center of the culture tank; The inlet and outlet are located at the bottom of the culture tank; The sliding rods are symmetrically installed on the side wall of the culture tank; The culture tank is suspended and fixed to the geometric center of the culture trough by a shock-absorbing bracket; The shock-absorbing bracket is fitted around the raised platform; The cap is threadedly connected to the opening of the culture tank and is pressed tightly against the top end face of the culture tank. It includes a vent with a microporous membrane and a sample inlet with a threaded sealing cap.

3. The temperature-controlled incubation device according to claim 1, characterized in that, The intelligent controller includes: a main control circuit board, a touch screen, and a USB interface; The main control circuit board, connected to the touch screen and the USB interface, includes: a high-performance microprocessor, a network communication module, a light control module, a temperature control module, a data storage module, and a wiring module; The wiring module is communicatively connected to the monitoring and execution component and is used to transmit sensor data and control signals; The high-performance microprocessor is equipped with a multi-level linkage PID algorithm, which acquires real-time environmental data through the network communication module to analyze the changing trends, dynamically adjusts the working strategies of the temperature control component and the lighting component, and adaptively adjusts the electronically controlled valve and the circulating pump based on the detection data of the liquid level sensor. The touchscreen is used to set the environmental parameters of the culture reactor; The USB interface is used to transmit data and signals.

4. The temperature-controlled incubation device according to claim 2, characterized in that, The monitoring components include a liquid level sensor and a temperature sensor; Both the liquid level sensor and the temperature sensor are fixed on the connecting sleeve; The wires of the liquid level sensor and the temperature sensor are both connected to the outlet along the extension direction of the sliding rod. The connecting sleeve is slidably fitted onto the sliding rod and locked in a preset position by a set screw; The actuators include a temperature control unit, a lighting unit, a circulating pump, and an electrically controlled valve; The outlet of the electrically controlled valve is sealed to the inlet and outlet of the water inlet; The circulating pump is fixed on a raised platform at the center of the bottom of the culture tank. The inlet of the circulating pump is parallel to the raised platform, and the outlet is perpendicular to the top. The temperature control component includes a heating module and a cooling module; The heating module is fixed inside the culture tank, and the cooling module is installed at the bottom of the culture tank and the bottom of the culture shell and is sealed and connected through an opening at the bottom of the culture tank. The lighting assembly includes a full-spectrum lamp and a lighting port; The full-spectrum lamp is spirally connected to the lighting port.

5. The temperature-controlled culture device according to claim 2, characterized in that, The shock-absorbing bracket includes a barrier and a shock-absorbing rod; The barrier is disposed on the side of the upper plane of the shock-absorbing support and is used to fix the culture tank; The shock absorber is used to provide elastic support and reduce vibration.

6. The temperature-controlled incubation device according to claim 4, characterized in that, The electrically controlled valve is a three-way ball valve with two inlets and one outlet. The first inlet of the electrically controlled valve is connected to an external water source pipeline, and the second inlet is connected to a drainage pipeline. The valve's rotation is controlled by an electromagnetic controller.

7. The temperature-controlled culture device according to claim 4, characterized in that, The temperature control component includes a heating module and a cooling module; The heating module includes a heating slot and a heating cartridge. The heating slot of the heating module has a rectangular opening and an elastic buckle on the inside. The heating cartridge is inserted into the heating slot from the side and is quickly fixed by the elastic buckle. The heating cartridge includes: a ceramic heating element and electrical contacts; The ceramic heating element is used for heating; The electrical contacts are used for signal transmission and power supply; The cooling module is used for refrigeration and includes a refrigeration compressor, a condenser, and a radiator. The refrigeration compressor and the radiator are installed at the bottom of the culture shell, and the condenser is installed at the bottom of the culture tank. The condenser is sealed to the refrigeration compressor and the radiator through an opening at the bottom of the culture tank.

8. A method of using a temperature-controlled culture device, applied to the temperature-controlled culture device according to any one of claims 1-7, characterized in that, include: The initial environmental parameters within each culture reactor are set using an intelligent controller. Environmental data within the culture reactor is acquired using monitoring and execution components; An intelligent controller is used to acquire environmental parameters of the target area via a network; An intelligent controller is used to dynamically adjust the environmental parameters of the culture reactor based on the environmental parameters of the target area and the environmental data inside the culture reactor. The monitoring and execution components are used to acquire water level data in each culture reactor; An intelligent controller is used to adjust the direction of the electrically controlled valves based on the water level data and preset water level range in the culture reactor.

9. The method of using the temperature-controlled culture device according to claim 8, characterized in that, The environmental parameters of the culture reactor include temperature and light parameters. The use of an intelligent controller to dynamically adjust the environmental parameters of the culture reactor based on the environmental parameters of the target area and the environmental data within the culture reactor includes: Calculate the temperature data of the baseline environmental water / soil based on the environmental parameters of the target area; The temperature data of the reference environmental water / soil is expressed as follows: in, The ambient surface temperature, The current value is the real-time weather temperature, C is the current cloud cover, P is the current precipitation, V is the current wind speed, and RH is the current relative humidity. K is atmospheric pressure, k1 is the dominant coefficient of temperature, k2 is the cloud cover-radiation correction coefficient, k3 is the precipitation cooling coefficient, k4 is the wind speed heat dissipation coefficient, k5 is the wind speed nonlinear exponent, k6 is the humidity evaporation correction coefficient, and k7 is the air pressure correction coefficient. The temperature parameters of the culture reactor are dynamically adjusted based on the pre-set target temperature difference and the temperature data of the reference environmental water / soil; the target temperature difference is the difference between the culture temperature data of the culture reactor and the temperature data of the reference environmental water / soil. The illumination parameters of the culture reactor are adjusted in conjunction with the illumination conditions of the target area and the temperature parameters.

10. The method of using the temperature-controlled culture device according to claim 8, characterized in that, The step of using an intelligent controller to adjust the direction of the electrically controlled valve by comparing the water level data in the culture reactor with a preset water level range includes: Compare the water level data in the culture reactor with the preset water level; When the water level in the culture reactor is lower than the preset water level, the electronically controlled valve is switched to the first inlet to supply water. When the water level in the culture reactor is higher than the preset water level, the electronically controlled valve is switched to the second inlet to drain water. If the water level in the culture reactor exceeds the preset water level for more than a preset time, the intelligent controller will issue an audible and visual alert.

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