A traditional chinese medicine anti-aging formula based on intestinal microecological regulation

Abstract

The application relates to the technical field of traditional Chinese medicine and prescription, and specifically discloses a traditional Chinese medicine and food homology anti-aging formula based on intestinal microecological regulation. The formula comprises the following raw materials: whey protein, calcium, vitamin D3, probiotics, prebiotics, postbiotics, vitamin C, vitamin E, polyphenols, Omega-3 fatty acids, astragalus polysaccharide, lycium barbarum polysaccharide, tanshinone, chromium, magnesium and dietary fiber; and the preparation method comprises the following steps: S1, astragalus, lycium barbarum and danshen are respectively crushed, sieved and extracted; S2, the probiotics are microencapsulated with the postbiotics; S3, the remaining raw materials are mixed with the extract; S4, the microencapsulated probiotic compound is mixed with a basic mixture; and S5, the compound particles are dried, granulated and mixed. The anti-aging formula can be used for preparing health care products or medicines for improving intestinal microecological imbalance and delaying body aging, and has the advantages of realizing intestinal microecological regulation and whole-body systematic anti-aging.

Description

Technical Field

[0001] This application relates to the fields of traditional Chinese medicine and formulary, and more specifically, it relates to a traditional Chinese medicine and food homology anti-aging formula based on intestinal microecological regulation. Background Technology

[0002] Traditional Chinese medicine (TCM) and its formulary techniques utilize principles and methods such as tonifying the liver and kidneys, strengthening the spleen and replenishing qi, promoting blood circulation and removing blood stasis, and resolving phlegm and unblocking collaterals. With the help of formulary combinations, they can regulate the body's neuro-endocrine-immune network through multiple targets and pathways, scavenge free radicals, improve metabolic function, and delay telomere shortening and cell aging. At the same time, TCM compound formulas emphasize the synergistic effect of the principal, assistant, adjuvant, and guide herbs, which can both support the body's vital energy and enhance the function of the internal organs to treat the root cause, and remove phlegm and blood stasis and other aging-related pathological products to treat the symptoms. Compared with single-component drugs, compound formulas have the characteristics of definite efficacy and fewer side effects, and have advantages in delaying physiological aging, improving age-related diseases, and improving the quality of life of the elderly.

[0003] Anti-aging products typically achieve antioxidant or metabolic regulation functions by adding small amounts of active ingredients, but they often overlook the complex interactions of multiple systems and targets during the aging process, resulting in limitations in improving overall physiological functions. At the same time, the formulation process lacks effective protection against heat-sensitive substances, which can easily lead to the loss of active ingredients during processing and storage, thereby affecting the final efficacy and stability of the product. Summary of the Invention

[0004] To address the limitation in improving overall physiological function caused by the addition of small amounts of active ingredients in related anti-aging products, this application provides a traditional Chinese medicine-food homology anti-aging formula based on intestinal microecological regulation.

[0005] This application provides a traditional Chinese medicine-food homology anti-aging formula based on intestinal microecology regulation, employing the following technical solution: A traditional Chinese medicine-food anti-aging formula based on gut microbiota regulation contains the following ingredients: whey protein, calcium, vitamin D3, probiotics, prebiotics, postbiotics, vitamin C, vitamin E, polyphenols, Omega-3 fatty acids, astragalus polysaccharide, wolfberry polysaccharide, tanshinone, chromium, magnesium and dietary fiber. The preparation method of the formula includes the following steps: S1. Astragalus membranaceus, Lycium barbarum and Salvia miltiorrhiza are pulverized, sieved and extracted to obtain extracts of Astragalus membranaceus polysaccharide, Lycium barbarum polysaccharide and Salvia miltiorrhiza ketone respectively; S2. Microencapsulate the probiotics and postbiotics to obtain a microencapsulated probiotic complex. S3. Mix whey protein, calcium, vitamin D3, vitamin C, vitamin E, polyphenols, Omega-3 fatty acids, chromium, magnesium and dietary fiber with the extract obtained in S1 to obtain a basic mixture; S4. Mix the microencapsulated probiotic complex obtained in step S2 with the basic mixture obtained in S3, add prebiotics, stir evenly, and obtain composite particles. S5. The composite particles obtained in S4 are dried, granulated, and mixed to produce the finished product.

[0006] By adopting the above technical solution, polysaccharides and ketone active ingredients extracted from Astragalus membranaceus, Lycium barbarum, and Salvia miltiorrhiza are combined with various vitamins, minerals, dietary fiber, and whey protein to construct a material basis that combines nutritional support and activity regulation. Microencapsulation technology is used to process probiotics and post-biotics, improving the stability of live bacteria during processing and storage. The above components are mixed and dried in a specific order. This preparation method considers the efficacy of heat-sensitive bioactive ingredients and the uniformity of mixing various materials. Therefore, a compound anti-aging product is obtained that can regulate the balance of intestinal flora through prebiotics, probiotics, and post-biotics, and exert antioxidant, anti-inflammatory, and metabolic support functions through various plant active ingredients and nutrients.

[0007] Preferably, the raw materials are in the following weight proportions: whey protein 7-13g, calcium 350-650mg, prebiotics 3.5-6.5g, post-biotics 0.7-1.3g, vitamin C 70-130mg, vitamin E 35-65mg, polyphenols 70-130mg, Omega-3 fatty acids 140-260mg, astragalus polysaccharide 70-130mg, wolfberry polysaccharide 35-65mg, tanshinone 35-65mg, chromium 14-26μg, magnesium 100-200mg, and dietary fiber 3.5-6.5g; while the vitamin D3 content is 600-1000IU and the probiotic content is 7×10 8 ~1.3×10 9 CFU.

[0008] By adopting the above technical solution, whey protein and minerals such as calcium, magnesium, and chromium are used to provide basic nutrition and metabolic support, with the dosage referencing the recommended daily intake range for adults. The combined dosage of vitamin C, vitamin E, polyphenols, and Omega-3 fatty acids aims to achieve synergistic antioxidant and anti-inflammatory effects. The dosage of astragalus polysaccharides, wolfberry polysaccharides, and tanshinone is set based on the theory of food and medicine homology in traditional Chinese medicine and the effective range of common extracts. The combined dosage of prebiotics, probiotics, and postbiotics aims to regulate the intestinal microecology. The number of live probiotics meets the conventional efficacy requirements of functional foods. Therefore, the proportions of each component in the obtained formula achieve the configuration of macronutrients, micronutrients, plant active ingredients, and microecological regulators within the safe dosage range, providing a quantitative basis for exerting comprehensive anti-aging effects.

[0009] Preferably, the probiotics are a combination of Lactobacillus rhamnosus and Bifidobacterium longum, with a live bacteria ratio of 1:1 to 3:1; the polyphenols are green tea extracts; and the weight ratio of vitamin C to vitamin E is 1.4:1 to 2.6:1.

[0010] By adopting the above technical solution, the combination of two strains, Lactobacillus rhamnosus and Bifidobacterium longum, which have research evidence supporting their ability to improve the intestinal barrier and regulate immune function, and their compounding at a specific live bacteria ratio, can promote synergistic colonization of the gut microbiota. Green tea extract rich in catechins is selected as the polyphenol source. At the same time, vitamin C and vitamin E are controlled within a specific ratio range. Vitamin C can regenerate oxidized vitamin E, thereby maintaining the system's continuous antioxidant capacity. Therefore, the resulting functional compound can scavenge free radicals, inhibit inflammation, and maintain intestinal health, enhancing the anti-aging physiological basis of the formula.

[0011] Preferably, in step S1, the extraction method of the astragalus polysaccharide is as follows: after pulverizing the astragalus medicinal material, add 8 to 12 times the mass of water, heat and reflux to extract 2 to 4 times, each time for 1 to 2 hours, combine the extracts, concentrate, precipitate with alcohol and dry to obtain astragalus polysaccharide extract.

[0012] By adopting the above technical solution, water is used as the extraction solvent, and by controlling the appropriate material-liquid ratio, extraction times and time, water-soluble polysaccharides in Astragalus membranaceus can be dissolved. The subsequent concentration and alcohol precipitation steps can separate and preliminarily purify the polysaccharides from the aqueous extract, removing impurities such as proteins and inorganic salts. Therefore, the purity of the obtained Astragalus membranaceus polysaccharide extract meets the requirements, and it can retain its immunomodulatory and antioxidant biological activities, providing active raw materials of traditional Chinese medicine for subsequent formulation mixing.

[0013] Preferably, in step S1, the extraction method of the wolfberry polysaccharide is as follows: after pulverizing the wolfberry medicinal material, add 6 to 10 times the mass of water or 30% to 50% ethanol by volume, heat and reflux to extract 2 to 4 times, each time for 1 to 2 hours, combine the extracts, concentrate, add 80% to 95% ethanol by volume for alcohol precipitation, stand, filter and dry to obtain wolfberry polysaccharide extract.

[0014] By adopting the above technical solution, the use of lower concentrations of ethanol or water for extraction facilitates the dissolution of polysaccharide components in wolfberry and reduces the carry-over of fat-soluble impurities. Controlling the heating and reflux conditions ensures extraction efficiency. Finally, high-concentration ethanol is used to precipitate the polysaccharides. This method has good selectivity, allowing wolfberry polysaccharides to precipitate from the extract. After filtration and drying, the extract is obtained. Therefore, the obtained wolfberry polysaccharide extract retains its nourishing and antioxidant effects and works synergistically with astragalus polysaccharides in subsequent formulations.

[0015] Preferably, in step S1, the extraction method of tanshinone is as follows: after pulverizing the tanshinone medicinal material, add 6 to 10 times the mass of 95% ethanol, heat and reflux to extract 2 to 4 times, each time for 1 to 2 hours, combine the extracts, recover the ethanol, concentrate and dry to obtain tanshinone extract.

[0016] By adopting the above technical solution, high-concentration ethanol is used as the extraction solvent. Based on the characteristic that tanshinone components are easily soluble in organic solvents, fat-soluble tanshinone can be extracted from the medicinal tissue. By setting up a multiple reflux extraction process, complete extraction is ensured. Subsequently, ethanol is recovered and concentrated and dried to obtain an extract with tanshinone as the main active ingredient. Therefore, the obtained tanshinone extract is enriched with fat-soluble components in tanshinone that improve circulation and have antioxidant activity. It complements the aforementioned water-soluble polysaccharide components and together constitutes the active substances of the food and medicine homology part of the formula.

[0017] Preferably, in step S2, the specific process of the microencapsulation treatment is as follows: First, the wall material is dissolved in deionized water to prepare a wall material solution with a mass fraction of 10% to 20%, wherein the wall material is at least one of gum arabic, gelatin, or sodium alginate; then, probiotic powder and postbiotics are mixed at a live bacteria ratio of 1:1 to 3:1 and added to the wall material solution, and stirred at 500 to 1000 rpm for 10 to 30 minutes at 4 to 10°C to form a uniform core material-wall material mixture; subsequently, it is dried using a spray drying device, with the inlet air temperature controlled at 120 to 160°C, the outlet air temperature at 60 to 80°C, the feed flow rate at 5 to 15 mL / min, and the atomization pressure at 0.1 to 0.2 MPa; after drying, a microencapsulated probiotic complex is obtained.

[0018] By adopting the above technical solution, edible polymer materials are used as wall materials, and probiotics and postbiotics are uniformly dispersed in the wall material solution under low-temperature stirring conditions to form an emulsion or suspension. Subsequently, spray drying technology is used to control the outlet air temperature and atomization conditions, so that the droplets form microcapsule solid particles that encapsulate the core material during the drying process. The microcapsule wall can block oxygen, humidity and adverse conditions that may be encountered in subsequent processes. Therefore, the obtained microencapsulated probiotic complex improves the survival rate and storage stability of probiotics, and enables the synergistic delivery of postbiotic components and live bacteria, providing support for the formulation to maintain microecological regulatory activity during subsequent mixing and product shelf life.

[0019] Preferably, in step S3, the mixing is specifically as follows: first, whey protein, dietary fiber and magnesium are added to a mixing container and premixed at 100-200 rpm for 5-10 minutes; then calcium, vitamin D3, vitamin C, vitamin E, polyphenols, Omega-3 fatty acids, chromium and the extract obtained in S1 are added, and the mixture is continued to be mixed at 200-300 rpm for 10-20 minutes.

[0020] By adopting the above technical solution, and using a stepwise mixing process, whey protein, dietary fiber, and magnesium, which have a relatively large proportion and similar fluidity, are first premixed to form a carrier matrix. This step helps to reduce the agglomeration of materials during subsequent mixing. Then, trace minerals, vitamins, and plant active ingredients are added to the premixed carrier, and the mixing speed and time are increased to ensure that all trace components can be evenly dispersed in the carrier. This mixing sequence and parameter settings take into account the differences in the physical properties and addition amounts of the materials. Therefore, the resulting basic mixture has uniform composition, which provides conditions for subsequent binding with microencapsulated probiotic complexes and prebiotics.

[0021] Preferably, in step S4, during the mixing process, the water content of the mixture is controlled to be 3% to 5%, the mixing time is 15 to 30 minutes, and the mixing speed is 50 to 100 rpm.

[0022] By adopting the above technical solution, the microencapsulated probiotic complex is mixed with the base mixture at a lower speed for a longer time, and the water content of the mixture is controlled within a lower range. The lower speed can reduce the mechanical shearing of the microcapsule structure and protect the activity of probiotics. The appropriate mixing time ensures that the prebiotics and each component are evenly distributed. The lower water content can prevent the material from clumping due to moisture absorption and inhibit the growth of microorganisms, reducing the burden on the subsequent drying process. Therefore, the obtained composite particles are loose and uniform, and the integrity of the microencapsulated probiotics is protected, which is conducive to maintaining the number of live probiotics in the product.

[0023] Preferably, in step S5, the drying temperature is 40 to 60°C, the drying time is 2 to 4 hours, and the particle size of the granulated particles is controlled to be 20 to 40 mesh.

[0024] By adopting the above technical solution, the final moisture content of the composite granules is reduced to a safe storage standard by using a mild drying temperature and time, while avoiding the impact of excessively high temperatures on heat-sensitive ingredients such as vitamins, probiotic microcapsules, and plant active ingredients. After drying, the particle size of the final product is controlled within a certain range through a granulation process. This particle size range ensures the product's flowability, facilitates dispensing and reconstitution, and also provides particle surface area, which is beneficial for disintegration and digestion after ingestion. Therefore, the obtained finished granules meet the requirements in terms of physical stability, retention of ingredient activity, and ease of use.

[0025] In summary, this application has the following beneficial effects: 1. This application utilizes polysaccharides and ketones extracted from Astragalus membranaceus, Lycium barbarum, and Salvia miltiorrhiza, respectively, and combines them with nutrients such as whey protein, vitamins, minerals, dietary fiber, and Omega-3 fatty acids to construct a complex system that combines nutritional support and bioactivity regulation. Simultaneously, probiotics and post-biotics are first microencapsulated to improve stability. Then, the microencapsulated product is mixed stepwise with a base mixture containing traditional Chinese medicine extracts and other components under low temperature and low shear conditions to avoid damage to live bacteria from high-intensity mixing operations. Considering the activity of heat-sensitive components, the resulting product achieves multi-target anti-aging intervention through the synergistic regulation of the intestinal microecology by prebiotics, probiotics, and post-biotics, and simultaneously exerts antioxidant, anti-inflammatory, and metabolic support functions through the synergistic effects of various phytochemicals and nutrients.

[0026] 2. This application preferably uses a combination of Lactobacillus rhamnosus and Bifidobacterium longum, and selects green tea extract, while using vitamin C and vitamin E as raw materials. Lactobacillus rhamnosus and Bifidobacterium longum colonize different ecological niches in the intestine and jointly support the intestinal barrier and immune function. In addition to their own antioxidant capacity, the polyphenols in green tea extract also act as prebiotics to promote the growth of the probiotics and are metabolized into active products by the intestinal flora. Vitamin C and vitamin E form a redox cycle in a certain ratio. Vitamin C can reduce oxidized vitamin E, thereby prolonging the antioxidant network action time of the entire formula. Therefore, the obtained compound enhances the anti-inflammatory, antioxidant and anti-aging effects through flora regulation-polyphenol metabolism-vitamin cycle.

[0027] 3. The method of this application first obtains water-soluble polysaccharides and fat-soluble tanshinone through water extraction and alcohol extraction processes, providing active raw materials for subsequent mixing; then, spray drying microencapsulation technology is used to block oxygen, heat and moisture, and mechanical stress, providing protection for functional units; subsequently, trace components are pre-dispersed using carriers such as whey protein and dietary fiber to ensure uniformity; then, microencapsulated probiotic complexes are introduced under low temperature, low speed, and controlled moisture conditions to reduce damage to probiotic microcapsules; finally, gentle drying and granulation further ensure the activity of product components, thereby achieving the goals of preserving probiotic activity, maintaining the stability of heat-sensitive and oxygen-sensitive components, and ensuring that each functional component is evenly distributed in the finished granules, thus exerting an anti-aging effect by regulating the intestinal microecology and providing nutritional support. Attached Figure Description

[0028] Figure 1 This is a flowchart illustrating the preparation method of a traditional Chinese medicine-food homology anti-aging formula based on gut microbiota regulation proposed in this application. Figure 2 This is a graph showing the results of the relative expression level detection of the col1a1a gene; Figure 3 This is a graph showing the results of the relative expression level detection of the eln1 gene; Figure 4 A statistical chart of skin elasticity; Figure 5 A statistical chart of wrinkle depth; Figure 6 A statistical chart of skin SOD activity; Figure 7 A statistical chart of skin GSH-Px activity. Detailed Implementation

[0029] The present application will be further described in detail below with reference to the accompanying drawings and embodiments.

[0030] Technical concept: Anti-aging products typically achieve antioxidant or metabolic regulation functions by adding small amounts of active ingredients, but they often overlook the complex interactions of multiple systems and targets during the aging process, resulting in limitations in improving overall physiological functions. At the same time, the formulation process lacks effective protection against heat-sensitive substances, which can easily lead to the loss of active ingredients during processing and storage, thereby affecting the final efficacy and stability of the product.

[0031] This application discloses a traditional Chinese medicine-food homology anti-aging formula based on intestinal microecological regulation. It contains the following ingredients: whey protein, calcium, vitamin D3, probiotics, prebiotics, postbiotics, vitamin C, vitamin E, polyphenols, Omega-3 fatty acids, astragalus polysaccharide, wolfberry polysaccharide, tanshinone, chromium, magnesium, and dietary fiber. The preparation method includes the following steps: S1, pulverizing, sieving, and extracting astragalus, wolfberry, and tanshinone respectively; S2, microencapsulating the probiotics and postbiotics; S3, mixing the remaining ingredients with the extract; S4, mixing the microencapsulated probiotic complex with the basic mixture; S5, drying, granulating, and mixing the composite particles.

[0032] This application utilizes polysaccharides and ketones extracted from Astragalus membranaceus, Lycium barbarum, and Salvia miltiorrhiza, respectively, and combines them with nutrients such as whey protein, vitamins, minerals, dietary fiber, and Omega-3 fatty acids to construct a complex system that combines nutritional support and bioactivity regulation. Simultaneously, probiotics and post-biotics are first microencapsulated to improve stability. Then, the microencapsulated products are mixed stepwise with a base mixture containing traditional Chinese medicine extracts and other components under low-temperature, low-shear conditions to avoid damage to live bacteria from high-intensity mixing operations. Consideration is also given to the activity of heat-sensitive components. Therefore, the resulting product synergistically regulates the intestinal microecology through prebiotics, probiotics, and post-biotics, while simultaneously exerting antioxidant, anti-inflammatory, and metabolic support functions through the synergistic effects of various phytochemicals and nutrients, achieving multi-target anti-aging intervention.

[0033] Example 1: This example provides a traditional Chinese medicine food-based anti-aging formula based on intestinal microecological regulation, which includes the following ingredients: whey protein, calcium, vitamin D3, probiotics, prebiotics, postbiotics, vitamin C, vitamin E, polyphenols, Omega-3 fatty acids, astragalus polysaccharide, wolfberry polysaccharide, tanshinone, chromium, magnesium and dietary fiber. The preparation method of the formula includes the following steps: S1. Astragalus membranaceus, Lycium barbarum and Salvia miltiorrhiza are pulverized, sieved and extracted to obtain extracts of Astragalus membranaceus polysaccharide, Lycium barbarum polysaccharide and Salvia miltiorrhiza ketone respectively; S2. Microencapsulate the probiotics and postbiotics to obtain a microencapsulated probiotic complex. S3. Mix whey protein, calcium, vitamin D3, vitamin C, vitamin E, polyphenols, Omega-3 fatty acids, chromium, magnesium and dietary fiber with the extract obtained in S1 to obtain a basic mixture; S4. Mix the microencapsulated probiotic complex obtained in step S2 with the basic mixture obtained in S3, add prebiotics, stir evenly, and obtain composite particles. S5. The composite particles obtained in S4 are dried, granulated, and mixed to produce the finished product.

[0034] The specific amounts of the ingredients are as follows: whey protein 7g, calcium 350mg, vitamin D3 600IU, and probiotics 7×10 8 CFU, prebiotics 3.5g, post-biotics 0.7g, vitamin C 70mg, vitamin E 35mg, polyphenols 70mg, Omega-3 fatty acids 140mg, astragalus polysaccharide 70mg, wolfberry polysaccharide 35mg, tanshinone 35mg, chromium 14μg, magnesium 100mg, dietary fiber 3.5g.

[0035] The probiotics are a combination of Lactobacillus rhamnosus and Bifidobacterium longum, with a live bacteria ratio of 1:1; while the polyphenols are green tea extract, and the weight ratio of vitamin C to vitamin E is 1.4:1.

[0036] In step S1, the extraction method of Astragalus polysaccharide is as follows: after pulverizing Astragalus medicinal material, add 8 times the mass of water, heat and reflux to extract twice, 1 hour each time, combine the extracts, concentrate, precipitate with alcohol and dry to obtain Astragalus polysaccharide extract.

[0037] In step S1, the extraction method of wolfberry polysaccharide is as follows: after crushing wolfberry medicinal material, add 6 times the mass of water, heat and reflux to extract twice, 1 hour each time, combine the extracts, concentrate, add 80% ethanol for alcohol precipitation, stand, filter and dry to obtain wolfberry polysaccharide extract.

[0038] In step S1, the extraction method of tanshinone is as follows: after pulverizing the tanshinone medicinal material, add 6 times the mass of 95% ethanol, heat and reflux to extract twice, 1 hour each time, combine the extracts, recover the ethanol, concentrate and dry to obtain tanshinone extract.

[0039] In step S2, the specific process of microencapsulation is as follows: First, the wall material is dissolved in deionized water to prepare a wall material solution with a mass fraction of 10%, and the wall material is gum arabic; then, probiotic powder and postbiotics are mixed at a live bacteria ratio of 1:1 and added to the wall material solution, and stirred at 500 rpm for 10 minutes at 4°C to form a uniform core material-wall material mixture; then, it is dried using a spray drying device, with the inlet air temperature controlled at 120°C, the outlet air temperature at 60°C, the feed flow rate at 5 mL / min, and the atomization pressure at 0.1 MPa; after drying, a microencapsulated probiotic complex is obtained.

[0040] In step S3, the mixing process is as follows: First, add whey protein, dietary fiber, and magnesium to a mixing container and premix at 100 rpm for 5 minutes; then add calcium, vitamin D3, vitamin C, vitamin E, polyphenols, Omega-3 fatty acids, chromium, and the extract obtained in step S1, and continue mixing at 200 rpm for 10 minutes.

[0041] In step S4, during the mixing process, the water content of the mixture is controlled at 3%, the mixing time is 15 minutes, and the mixing speed is 50 rpm.

[0042] In step S5, the drying temperature is 40°C, the drying time is 2 hours, and the particle size of the granulated particles is controlled at 20 mesh.

[0043] Example 2: This example provides a traditional Chinese medicine food-based anti-aging formula based on intestinal microecological regulation, which includes the following ingredients: whey protein, calcium, vitamin D3, probiotics, prebiotics, postbiotics, vitamin C, vitamin E, polyphenols, Omega-3 fatty acids, astragalus polysaccharide, wolfberry polysaccharide, tanshinone, chromium, magnesium and dietary fiber. The preparation method of the formula includes the following steps: S1. Astragalus membranaceus, Lycium barbarum and Salvia miltiorrhiza are pulverized, sieved and extracted to obtain extracts of Astragalus membranaceus polysaccharide, Lycium barbarum polysaccharide and Salvia miltiorrhiza ketone respectively; S2. Microencapsulate the probiotics and postbiotics to obtain a microencapsulated probiotic complex. S3. Mix whey protein, calcium, vitamin D3, vitamin C, vitamin E, polyphenols, Omega-3 fatty acids, chromium, magnesium and dietary fiber with the extract obtained in S1 to obtain a basic mixture; S4. Mix the microencapsulated probiotic complex obtained in step S2 with the basic mixture obtained in S3, add prebiotics, stir evenly, and obtain composite particles. S5. The composite particles obtained in S4 are dried, granulated, and mixed to produce the finished product.

[0044] The specific amounts of the ingredients are as follows: 10g whey protein, 500mg calcium, 800IU vitamin D3, and 1.0×10 probiotics. 9 CFU, prebiotics 5g, post-biotics 1.0g, vitamin C 100mg, vitamin E 50mg, polyphenols 100mg, Omega-3 fatty acids 200mg, astragalus polysaccharide 100mg, wolfberry polysaccharide 50mg, tanshinone 50mg, chromium 20μg, magnesium 150mg, dietary fiber 5g.

[0045] The probiotics are a combination of Lactobacillus rhamnosus and Bifidobacterium longum, with a live bacteria ratio of 2:1; while the polyphenols are green tea extract, and the weight ratio of vitamin C to vitamin E is 2.0:1.

[0046] In step S1, the extraction method of Astragalus polysaccharide is as follows: after pulverizing Astragalus medicinal material, add 10 times the mass of water, heat and reflux to extract 3 times, each time for 1.5 hours, combine the extracts, concentrate, precipitate with alcohol and dry to obtain Astragalus polysaccharide extract.

[0047] In step S1, the extraction method of wolfberry polysaccharide is as follows: after crushing wolfberry medicinal material, add 8 times the mass of 40% ethanol, heat and reflux to extract 3 times, 1.5 hours each time, combine the extracts, concentrate, add 85% ethanol for alcohol precipitation, stand, filter and dry to obtain wolfberry polysaccharide extract.

[0048] In step S1, the extraction method of tanshinone is as follows: after pulverizing the tanshinone medicinal material, add 8 times the mass of 95% ethanol, heat and reflux to extract 3 times, each time for 1.5 hours, combine the extracts, recover the ethanol, concentrate and dry to obtain tanshinone extract.

[0049] In step S2, the specific process of microencapsulation is as follows: First, the wall material is dissolved in deionized water to prepare a wall material solution with a mass fraction of 15%, and the wall material is gelatin; then, probiotic powder and postbiotics are mixed at a live bacteria ratio of 2:1 and added to the wall material solution, and stirred at 750 rpm for 20 minutes at 7°C to form a uniform core material-wall material mixture; then, it is dried using a spray drying device, with the inlet air temperature controlled at 140°C, the outlet air temperature at 70°C, the feed flow rate at 10 mL / min, and the atomization pressure at 0.15 MPa; after drying, a microencapsulated probiotic complex is obtained.

[0050] In step S3, the mixing process is as follows: First, add whey protein, dietary fiber, and magnesium to a mixing container and premix at 150 rpm for 7 minutes; then add calcium, vitamin D3, vitamin C, vitamin E, polyphenols, Omega-3 fatty acids, chromium, and the extract obtained in step S1, and continue mixing at 250 rpm for 15 minutes.

[0051] In step S4, during the mixing process, the water content of the mixture is controlled at 4%, the mixing time is 22 minutes, and the mixing speed is 75 rpm.

[0052] In step S5, the drying temperature is 50°C, the drying time is 3 hours, and the particle size of the granulated particles is controlled at 30 mesh.

[0053] Example 3: This example provides a traditional Chinese medicine food-based anti-aging formula based on intestinal microecological regulation, which includes the following ingredients: whey protein, calcium, vitamin D3, probiotics, prebiotics, postbiotics, vitamin C, vitamin E, polyphenols, Omega-3 fatty acids, astragalus polysaccharide, wolfberry polysaccharide, tanshinone, chromium, magnesium and dietary fiber. The preparation method of the formula includes the following steps: S1. Astragalus membranaceus, Lycium barbarum and Salvia miltiorrhiza are pulverized, sieved and extracted to obtain extracts of Astragalus membranaceus polysaccharide, Lycium barbarum polysaccharide and Salvia miltiorrhiza ketone respectively; S2. Microencapsulate the probiotics and postbiotics to obtain a microencapsulated probiotic complex. S3. Mix whey protein, calcium, vitamin D3, vitamin C, vitamin E, polyphenols, Omega-3 fatty acids, chromium, magnesium and dietary fiber with the extract obtained in S1 to obtain a basic mixture; S4. Mix the microencapsulated probiotic complex obtained in step S2 with the basic mixture obtained in S3, add prebiotics, stir evenly, and obtain composite particles. S5. The composite particles obtained in S4 are dried, granulated, and mixed to produce the finished product.

[0054] The specific amounts of the ingredients are as follows: whey protein 13g, calcium 650mg, vitamin D3 1000IU, and probiotics 1.3×10⁻⁶. 9 CFU, prebiotics 6.5g, post-biotics 1.3g, vitamin C 130mg, vitamin E 65mg, polyphenols 130mg, Omega-3 fatty acids 260mg, astragalus polysaccharide 130mg, wolfberry polysaccharide 65mg, tanshinone 65mg, chromium 26μg, magnesium 200mg, dietary fiber 6.5g.

[0055] The probiotics are a combination of Lactobacillus rhamnosus and Bifidobacterium longum, with a live bacteria ratio of 3:1; while the polyphenols are green tea extract, and the weight ratio of vitamin C to vitamin E is 2.6:1.

[0056] In step S1, the extraction method of Astragalus polysaccharide is as follows: after pulverizing Astragalus medicinal material, add 12 times the mass of water, heat and reflux to extract 4 times, 2 hours each time, combine the extracts, concentrate, precipitate with alcohol and dry to obtain Astragalus polysaccharide extract.

[0057] In step S1, the extraction method of wolfberry polysaccharide is as follows: after crushing wolfberry medicinal material, add 10 times the mass of 50% ethanol, heat and reflux to extract 4 times, 2 hours each time, combine the extracts, concentrate, add 95% ethanol for alcohol precipitation, stand, filter and dry to obtain wolfberry polysaccharide extract.

[0058] In step S1, the extraction method of tanshinone is as follows: after pulverizing the tanshinone medicinal material, add 10 times the mass of 95% ethanol, heat and reflux to extract 4 times, 2 hours each time, combine the extracts, recover the ethanol, concentrate and dry to obtain tanshinone extract.

[0059] In step S2, the microencapsulation process is as follows: First, the wall material is dissolved in deionized water to prepare a wall material solution with a mass fraction of 20%, and the wall material is sodium alginate; then, probiotic powder and post-biotic are mixed at a live bacteria ratio of 3:1 and added to the wall material solution, and stirred at 1000 rpm for 30 minutes at 10℃ to form a uniform core material-wall material mixture; then, it is dried using a spray drying device, with the inlet air temperature controlled at 160℃, the outlet air temperature at 80℃, the feed flow rate at 15mL / min, and the atomization pressure at 0.2MPa; after drying, a microencapsulated probiotic complex is obtained.

[0060] In step S3, the mixing process is as follows: First, add whey protein, dietary fiber, and magnesium to a mixing container and premix at 200 rpm for 10 minutes; then add calcium, vitamin D3, vitamin C, vitamin E, polyphenols, Omega-3 fatty acids, chromium, and the extract obtained in step S1, and continue mixing at 300 rpm for 20 minutes.

[0061] In step S4, during the mixing process, the water content of the mixture is controlled at 5%, the mixing time is 30 minutes, and the mixing speed is 100 rpm.

[0062] In step S5, the drying temperature is 60°C, the drying time is 4 hours, and the particle size of the granulated particles is controlled at 40 mesh.

[0063] Example 4: Zebrafish were randomly selected and placed in 6-well plates, 30 fish per well. The experimental groups were given the sample prepared according to the formulation in Example 1, and a normal control group was also included. The volume per well was 3 mL. Three biological replicates were performed. After incubation at 28°C in the dark for 24 hours, total RNA was extracted from each group of zebrafish, and cDNA was synthesized. The expression of β-actin and the target gene was detected using q-PCR. β-actin was used as an internal control for gene expression, and the relative RNA expression levels of the col1a1a and eln1 genes were calculated. The final results are shown below. Figure 2 and Figure 3 As shown.

[0064] A comparative experiment was conducted with 30 zebrafish in each group, and the results were analyzed. Figure 2 and Figure 3 As a result, compared with the normal control group, the relative RNA expression levels of the col1a1a and eln1 genes were significantly increased in the experimental group.

[0065] This embodiment confirms that the traditional Chinese medicine and food homology anti-aging formula based on intestinal microecology regulation proposed in this application has anti-aging effects.

[0066] Example 5: This example provides a traditional Chinese medicine-based anti-aging formula based on gut microbiota regulation. One hundred female volunteers aged 38.50±12.6 years were recruited for the experiment. Inclusion criteria were: meeting the criteria for facial photoaging (Glogau photoaging classification II-IV, clinically including rough skin, wrinkles, enlarged pores, dull skin tone, abnormal pigmentation, telangiectasia, etc.); and meeting the criteria for Fitzpatrick skin classification III-IV; and being able to cooperate with the treatment plan of this study. Exclusion criteria were: pregnant or lactating women; those with other skin diseases at the test site; those with a history of allergy to the ingredients in the product; those with infections at the test site; those with serious systemic diseases that would affect the interpretation of results; those who had received any cosmetic treatment at the test site within the past 3 months; and those who had taken oral retinoids within the past 3 months.

[0067] The specific implementation method of the experiment is as follows: volunteers are randomly divided into a control group and an experimental group, with 50 people in each group. The control group consumes dietary powder that does not have similar effects every day; the volunteers in the experimental group consume the sample obtained according to the formula in Example 1 every day, 4 portions each time, with an appropriate amount of warm water added and mixed evenly before drinking, for 2 consecutive weeks, during which time other skin care products are discontinued.

[0068] The criteria for judging the efficacy of the experiment were: Skin elasticity (R2 value): The skin elasticity (R2 value) at the location 1 cm below the corners of the eyes (crow's feet area) was measured using a multifunctional skin physiology tester before the experiment (day 0) and after the experiment (week 2) using the suction stretching method.

[0069] Wrinkle depth (Ra value): Radial wrinkles at the outer corner of the eye (crow's feet) were measured before (day 0) and after (week 2) using a high-resolution ultraviolet camera and image analysis software to perform three-dimensional reconstruction of the skin surface and calculate the average wrinkle depth (Ra value, unit μm). The lower the Ra value, the shallower the wrinkle.

[0070] Skin SOD activity (U / mg protein): D-Squame® tape was applied five times consecutively to a designated area (cheekbone region) to collect the outermost layer of keratinocytes. The ability of SOD in the sample to inhibit the reaction of WST-8 with superoxide anions to produce nailbinoids was detected using a kit, and the enzyme activity was calculated.

[0071] Skin GSH-Px activity (mU / mg protein): Samples were collected using the same adhesive tape peeling method. GSH-Px catalyzes the reaction between glutathione (GSH) and hydrogen peroxide. The resulting oxidized glutathione glutathione (GSSG) can be reduced back to GSH under the action of glutathione reductase and NADPH, while NADPH is oxidized to NADP+. GSH-Px activity can be calculated by detecting the rate of decrease in NADPH absorbance at 340 nm.

[0072] The experimental data are expressed as mean ± sem. Data analysis was performed using GraphPadPrism software, and paired-samples t-tests were used. A p-value < 0.05 was considered statistically significant. The results are statistically significant. Figures 4-7 And Tables 1-4.

[0073] Table 1: Statistics on Skin Elasticity (R² Value) (n=100)

[0074] Depend on Figure 4 As shown in Table 1, compared with the control group, the skin elasticity (R2 value) of the crow's feet area 1 cm below the corners of the eyes in the experimental group volunteers increased significantly, with an increase of 31.0% after 2 weeks, which was extremely significant compared with the control group (P<0.001).

[0075] Table 2: Statistics on Wrinkle Depth (Ra Value) (n=100)

[0076] Depend on Figure 5 As shown in Table 2, compared with the control group, the wrinkle depth (Ra value) at the outer corner of the eye of the volunteers in the experimental group was significantly shallower, decreasing by 30.5% after 2 weeks, which was highly significant compared with the control group (P<0.001). Table 3: Statistics on Skin SOD Activity (U / mg Protein) (n=100)

[0077] Depend on Figure 6 As shown in Table 3, compared with the control group, the SOD activity in the cheek skin of the experimental group volunteers was significantly activated, increasing by 25.9% after 2 weeks, which was extremely significant compared with the control group (P<0.001).

[0078] Table 4: Statistical table of skin GSH-Px activity (mU / mg protein) (n=100)

[0079] Depend on Figure 7 As shown in Table 4, compared with the control group, the GSH-Px activity in the zygomatic skin of the experimental group volunteers was significantly activated, increasing by 30.9% after 2 weeks, which was highly significant compared with the control group (P<0.001).

[0080] After a two-week comparative experiment, the traditional Chinese medicine and food-based anti-aging formula based on gut microbiota regulation proposed in this application significantly improved skin elasticity and reversed skin sagging; reduced wrinkle depth and improved skin smoothness; enhanced the activity of the two core antioxidant enzymes in the skin (SOD and GSH-Px), and strengthened the skin's own ability to resist oxidative damage. It can effectively improve aging-related symptoms such as rough skin, the number and depth of wrinkles, enlarged pores, dull skin tone, and age spots. All subjects completed the experiment without any adverse reaction reports, and the product has good safety and tolerability.

[0081] This study confirms that the traditional Chinese medicine-food homology anti-aging formula proposed in this application, based on gut microbiota regulation, effectively achieves comprehensive anti-aging effects from the inside out by enhancing the skin's endogenous antioxidant defense system—a core mechanism.

[0082] This specific embodiment is merely an explanation of this application and is not intended to limit it. After reading this specification, those skilled in the art can make modifications to this embodiment without contributing any inventive step, but such modifications are protected by patent law as long as they fall within the scope of the claims of this application.

Claims

1. A traditional Chinese medicine-food homology anti-aging formula based on gut microbiota regulation, characterized in that, It contains the following ingredients: whey protein, calcium, vitamin D3, probiotics, prebiotics, postbiotics, vitamin C, vitamin E, polyphenols, Omega-3 fatty acids, astragalus polysaccharide, wolfberry polysaccharide, tanshinone, chromium, magnesium and dietary fiber; The preparation method of the formula includes the following steps: S1. Astragalus membranaceus, Lycium barbarum and Salvia miltiorrhiza are pulverized, sieved and extracted to obtain extracts of Astragalus membranaceus polysaccharide, Lycium barbarum polysaccharide and Salvia miltiorrhiza ketone respectively; S2. Microencapsulate the probiotics and postbiotics to obtain a microencapsulated probiotic complex. S3. Mix whey protein, calcium, vitamin D3, vitamin C, vitamin E, polyphenols, Omega-3 fatty acids, chromium, magnesium and dietary fiber with the extract obtained in S1 to obtain a basic mixture; S4. Mix the microencapsulated probiotic complex obtained in step S2 with the basic mixture obtained in S3, add prebiotics, stir evenly, and obtain composite particles. S5. The composite particles obtained in S4 are dried, granulated, and mixed to produce the finished product.

2. The traditional Chinese medicine-food homology anti-aging formula based on intestinal microecological regulation according to claim 1, characterized in that, The raw materials are present in the following weight proportions: whey protein 7-13g, calcium 350-650mg, prebiotics 3.5-6.5g, post-biotics 0.7-1.3g, vitamin C 70-130mg, vitamin E 35-65mg, polyphenols 70-130mg, Omega-3 fatty acids 140-260mg, astragalus polysaccharide 70-130mg, wolfberry polysaccharide 35-65mg, tanshinone 35-65mg, chromium 14-26μg, magnesium 100-200mg, and dietary fiber 3.5-6.5g; while the vitamin D3 content is 600-1000IU and the probiotic content is 7×10 8 ~1.3×10 9 CFU.

3. The traditional Chinese medicine-food homology anti-aging formula based on intestinal microecological regulation according to claim 1, characterized in that, The probiotics are a combination of Lactobacillus rhamnosus and Bifidobacterium longum, with a live bacteria ratio of 1:1 to 3:1; the polyphenols are green tea extract; and the weight ratio of vitamin C to vitamin E is 1.4:1 to 2.6:

1.

4. The traditional Chinese medicine-food homology anti-aging formula based on intestinal microecological regulation according to claim 1, characterized in that, In step S1, the extraction method of Astragalus polysaccharide is as follows: after pulverizing Astragalus medicinal material, add 8 to 12 times the mass of water, heat and reflux to extract 2 to 4 times, each time for 1 to 2 hours, combine the extracts, concentrate, precipitate with alcohol and dry to obtain Astragalus polysaccharide extract.

5. The traditional Chinese medicine-food homology anti-aging formula based on intestinal microecological regulation according to claim 1, characterized in that, In step S1, the extraction method of the wolfberry polysaccharide is as follows: after crushing the wolfberry medicinal material, add 6 to 10 times the mass of water or 30% to 50% ethanol by volume, heat and reflux to extract 2 to 4 times, each time for 1 to 2 hours, combine the extracts, concentrate, add 80% to 95% ethanol by volume for alcohol precipitation, stand, filter and dry to obtain wolfberry polysaccharide extract.

6. The traditional Chinese medicine-food homology anti-aging formula based on intestinal microecological regulation according to claim 1, characterized in that, In step S1, the extraction method of tanshinone is as follows: after pulverizing the tanshinone medicinal material, add 6 to 10 times the mass of 95% ethanol, heat and reflux to extract 2 to 4 times, each time for 1 to 2 hours, combine the extracts, recover the ethanol, concentrate and dry to obtain tanshinone extract.

7. The traditional Chinese medicine-food homology anti-aging formula based on intestinal microecological regulation according to claim 1, characterized in that, In step S2, the specific process of microencapsulation is as follows: First, the wall material is dissolved in deionized water to prepare a wall material solution with a mass fraction of 10% to 20%, wherein the wall material is at least one of gum arabic, gelatin, or sodium alginate; then, probiotic powder and postbiotics are mixed at a live bacteria ratio of 1:1 to 3:1 and added to the wall material solution, and stirred at 500 to 1000 rpm for 10 to 30 minutes at 4 to 10°C to form a uniform core material-wall material mixture; subsequently, it is dried using a spray drying device, with the inlet air temperature controlled at 120 to 160°C, the outlet air temperature at 60 to 80°C, the feed flow rate at 5 to 15 mL / min, and the atomization pressure at 0.1 to 0.2 MPa; after drying, a microencapsulated probiotic complex is obtained.

8. A traditional Chinese medicine-food homology anti-aging formula based on intestinal microecological regulation according to claim 1, characterized in that, In step S3, the mixing is as follows: First, whey protein, dietary fiber and magnesium are added to a mixing container and premixed at 100-200 rpm for 5-10 minutes; then calcium, vitamin D3, vitamin C, vitamin E, polyphenols, Omega-3 fatty acids, chromium and the extract obtained in S1 are added, and the mixture is continued to be mixed at 200-300 rpm for 10-20 minutes.

9. A traditional Chinese medicine-food homology anti-aging formula based on intestinal microecological regulation according to claim 1, characterized in that, In step S4, during the mixing process, the water content of the mixture is controlled to be 3% to 5%, the mixing time is 15 to 30 minutes, and the mixing speed is 50 to 100 rpm.

10. A traditional Chinese medicine-food homology anti-aging formula based on intestinal microecological regulation according to claim 1, characterized in that, In step S5, the drying temperature is 40 to 60°C, the drying time is 2 to 4 hours, and the particle size of the granulated particles is controlled to be 20 to 40 mesh.

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