Hydrotalcite nanocomposite, preparation method and application thereof

Abstract

The application discloses a hydrotalcite nanocomposite, a preparation method and application thereof. The composite comprises a high-entropy hydrotalcite nanosheet matrix and platinum nanoclusters loaded on the matrix; and cations in the layer plate of the high-entropy hydrotalcite nanosheet matrix comprise cations of Cu, Mn, Co, Ni and Fe. A nanomedicine for treating tumors prepared by using the hydrotalcite nanocomposite has excellent catalytic ROS generation capacity.

Description

Technical Field

[0001] This invention relates to the field of tumor drug or magnetic resonance imaging agent preparation technology. More specifically, it relates to a hydrotalcite nanocomposite material, its preparation method, and its applications. Background Technology

[0002] Reactive oxygen species (ROS) are widely present in living organisms and include hydroxyl radicals (·OH) and superoxide (O2). - ), singlet oxygen ( 1 Intracellular reactive oxygen species (ROS) include oxygen (O2) and hydrogen peroxide (H2O2). They play a crucial role in physiological functions, regulating proteins, producing hormones, modulating cell signaling, mediating inflammation, and eliminating pathogens. However, excessive intracellular ROS can induce non-specific damage to proteins, lipids, and DNA; therefore, ROS-based cancer therapies have been extensively explored in recent years (Journal of Nanobiotechnology. 2021, 19, 206).

[0003] Catalytic therapy is a novel cancer treatment method that has emerged in recent years because it can generate ROS in response to the tumor microenvironment, effectively removing tumors deep within tissues. Therefore, the development of highly efficient nanozymes has become one of the key issues in the field of catalytic therapy (Advanced Healthcare Materials. 2023, 12, 2302056). Since the first report in 2007 that Fe3O4 nanoparticles exhibited activity similar to natural horseradish peroxidase, various nanomaterials have been designed and developed into nanozymes, including noble metals (such as Pt, Au, and Pd), metal oxides (such as MnO2, MoO3, WO3, Co3O4, Cu2O, and CeO2), layered double hydroxides (hydrotalcite, LDHs), carbon nanomaterials, and transition metal chalcogenides (such as MoS2, FeS2, and MoSe2). Among these, hydrotalcite has attracted much attention as a nanozyme for catalytic therapy due to its ease of preparation and modification, tunable properties, good biocompatibility, and biodegradability. For example, an advanced cobalt ferrite layered double hydroxide sandwich nanozyme for ROS blooming tumor therapy has been reported (Chemical Engineering Journal. 2023, 473, 145414). In recent years, high-entropy layered double hydroxide nanomaterials have shown great potential as nanozymes due to their tunable electronic properties and high catalytic activity (ACS Catalysis. 2023, 13, 7698-7706). However, the ability of previously reported high-entropy layered double hydroxide nanomaterials to catalyze ROS generation in the tumor microenvironment needs further improvement, which greatly limits their further application as nanozymes in catalytic therapy. Summary of the Invention

[0004] Therefore, the purpose of this invention is to provide a hydrotalcite nanocomposite material, its preparation method, and its applications. The nanomedicines for treating tumors prepared using this hydrotalcite nanocomposite material exhibit excellent catalytic ability to generate ROS.

[0005] To achieve the above objectives, the present invention adopts the following technical solution:

[0006] On one hand, the present invention provides a hydrotalcite nanocomposite material, wherein the composite material comprises a high-entropy hydrotalcite nanosheet matrix and platinum nanoclusters loaded on the matrix;

[0007] The cations in the layers of the high-entropy hydrotalcite nanosheet matrix include Cu, Mn, Co, Ni, and Fe cations.

[0008] That is, in the layers of the high-entropy hydrotalcite nanosheet matrix, Cu, Mn, Co, Ni and Fe all exist in the form of cations.

[0009] Furthermore, the platinum nanoclusters are uniformly distributed on the high-entropy hydrotalcite nanosheet matrix.

[0010] In the technical solution of this invention, the loading of platinum nanoclusters has no significant effect on the morphology and size of the hydrotalcite nanosheets, only the thickness increases slightly.

[0011] Furthermore, in the layers of the high-entropy hydrotalcite nanosheet matrix, the molar ratio of each metal cation is 1:1:1:1:1.

[0012] Furthermore, the high-entropy hydrotalcite nanosheet matrix has a length and width of 50-150 nm and a thickness of 3-4 nm.

[0013] Furthermore, the composite material has a length and width of 50-150 nm and a thickness of 5-7 nm.

[0014] Furthermore, in the composite material, the molar content of platinum nanoclusters is 3-5%.

[0015] In another aspect, the present invention provides a method for preparing the hydrotalcite nanocomposite material as described above, the method comprising the following steps:

[0016] An aqueous solution of a soluble metal salt and an aqueous solution of sodium hydroxide were simultaneously added to an aqueous solution containing a hydrotalcite growth inhibitor and sodium nitrate. The mixture was stirred at 50-100℃ for 0.5-6 hours, centrifuged, washed, and dried to obtain high-entropy copper-manganese-cobalt-nickel-iron hydrotalcite nanosheets.

[0017] The high-entropy copper-manganese-cobalt-nickel-iron hydrotalcite nanosheets were dispersed in water to obtain a colloid;

[0018] An aqueous solution of chloroplatinic acid hexahydrate was added to the colloid and stirred until homogeneous to obtain a mixture.

[0019] An aqueous solution of sodium borohydride containing potassium hydroxide was added dropwise to the mixture, and an impregnation and reduction reaction was carried out under stirring. After centrifugation, washing, separation, and grinding of the precipitate, the hydrotalcite nanocomposite material was obtained.

[0020] Furthermore, the hydrotalcite growth inhibitor is selected from polyethylene glycol and / or formamide. Adding the hydrotalcite growth inhibitor can inhibit the growth of hydrotalcite layers, which is beneficial for the formation of small, thin-layered hydrotalcite.

[0021] Furthermore, the polyethylene glycol is selected from polyethylene glycol 400.

[0022] Furthermore, in the aqueous solution of sodium borohydride containing potassium hydroxide, the concentration of potassium hydroxide is 1.5-2.5M, preferably 2M.

[0023] Furthermore, in the aqueous solution of sodium borohydride containing dissolved potassium hydroxide, the sodium borohydride reacts with the Pt in the mixture. 2+ The molar ratio is 40:1.

[0024] Furthermore, the soluble salt of the metal is a soluble salt of Cu, Mn, Co, Ni, and Fe.

[0025] Furthermore, the soluble salt of the metal is a mixture of copper nitrate, manganese chloride, cobalt nitrate, nickel nitrate, and ferric nitrate.

[0026] Furthermore, the volume ratio of the aqueous solution of the soluble salt of the metal to the aqueous solution of sodium hydroxide is 1:(0.8-1.5).

[0027] Furthermore, in the mixture containing hydrotalcite growth inhibitor and sodium nitrate, the volume ratio of hydrotalcite growth inhibitor to water is 1:3-1:4, and the concentration of sodium nitrate is 12-20 mg / mL, preferably 17 mg / mL.

[0028] Furthermore, the mass ratio of high-entropy copper-manganese-cobalt-nickel-iron hydrotalcite nanosheets to chloroplatinic acid hexahydrate in the colloid is 5-25%.

[0029] Furthermore, the impregnation reduction reaction is carried out at a temperature of 20-30°C for 3-8 hours.

[0030] In another aspect, the present invention provides the application of the hydrotalcite nanocomposite material as described above in the preparation of nanomedicines or nuclear magnetic resonance imaging agents.

[0031] Unless otherwise specified, all raw materials used in this invention can be purchased commercially or obtained through conventional means in the field.

[0032] The beneficial effects of this invention are as follows:

[0033] In the hydrotalcite nanocomposite material provided by this invention, platinum nanoclusters are uniformly loaded on high-entropy hydrotalcite nanosheets. Due to the two-dimensional confinement effect of the high-entropy hydrotalcite nanosheets, the generation efficiency of singlet oxygen and superoxide radicals in metallic platinum can be effectively increased, enabling the efficient generation of reactive oxygen species in the tumor microenvironment. Furthermore, it exhibits good biocompatibility, demonstrating excellent tumor therapeutic effects while possessing MRI imaging capabilities, with low toxicity and good cancer treatment efficacy.

[0034] The preparation method of the hydrotalcite nanocomposite material provided in this invention uses high-entropy hydrotalcite nanosheets as therapeutic drugs and carriers. The composite material is obtained by mechanically stirring a platinum source solution with a hydrotalcite nanosheet colloidal solution, followed by further impregnation with sodium borohydride and centrifugation. This method is simple, energy-efficient, requires minimal equipment, and is environmentally friendly. Attached Figure Description

[0035] The specific embodiments of the present invention will be described in further detail below with reference to the accompanying drawings.

[0036] Figure 1 The image shown is a transmission electron microscope (TEM) image of the platinum nanoclusters / high-entropy hydrotalcite nanocomposite material synthesized in Example 1.

[0037] Figure 2 The X-ray diffraction pattern of the platinum nanoclusters / high-entropy hydrotalcite nanocomposite material synthesized in Example 1 is shown.

[0038] Figure 3 The electron spin resonance diagram of the platinum nanoclusters / high-entropy hydrotalcite nanocomposite material synthesized in Example 1 is shown.

[0039] Figure 4 The image shows the UV spectrum of reactive oxygen species generated in the platinum nanoclusters / high-entropy hydrotalcite nanocomposite material synthesized in Example 1.

[0040] Figure 5 The L929 cell compatibility diagram of the platinum nanoclusters / high-entropy hydrotalcite nanocomposite material synthesized in Example 1 is shown.

[0041] Figure 6 The image shows the nuclear magnetic resonance imaging (NMR) result of the platinum nanoclusters / high-entropy hydrotalcite nanocomposite material synthesized in Example 1.

[0042] Figure 7 The image shows a UV comparison of the reactive oxygen species generated by the platinum nanoclusters / high-entropy hydrotalcite nanocomposite material synthesized in Example 1 and the platinum nanoclusters / nickel-iron hydrotalcite nanocomposite material. Detailed Implementation

[0043] To more clearly illustrate the present invention, the following description, in conjunction with preferred embodiments and accompanying drawings, further explains the invention. Similar components in the drawings are indicated by the same reference numerals. Those skilled in the art should understand that the specific description below is illustrative rather than restrictive and should not be construed as limiting the scope of protection of the present invention.

[0044] Example 1

[0045] The preparation steps of a platinum nanoclusters / high-entropy hydrotalcite nanocomposite material are as follows:

[0046] 1) Dissolve 0.79g manganese chloride tetrahydrate, 1.62g ferric nitrate nonahydrate, 1.16g cobalt nitrate hexahydrate, 1.16g nickel nitrate hexahydrate, and 0.97g copper nitrate trihydrate in 100mL of water and mix well to obtain solution A; dissolve 1.70g sodium nitrate in a mixture containing 25mL polyethylene glycol 400 and 75mL water and mix well to obtain solution B; dissolve 2.0g sodium hydroxide in 100mL of water and mix well to obtain solution C; under stirring in an 80℃ water bath, slowly add solutions A and C to solution B simultaneously, adjust the pH to 9, react for 0.5h, centrifuge, wash, freeze-dry, grind and sieve to obtain a dark brown powder, which is the high-entropy hydrotalcite nanosheet;

[0047] 2) Take 1g of the high-entropy hydrotalcite nanosheets obtained in step 1) and dissolve them in 50ml of deionized water, stirring mechanically for about 30min; dissolve 0.1110g of chloroplatinic acid hexahydrate in 20ml of deionized water, then add the dissolved chloroplatinic acid hexahydrate solution to the uniformly dispersed hydrotalcite nanosheet colloid, and continue stirring for 1h; then add 0.745g of sodium borohydride (nNaBH4=40nPt) 2+ Dissolve the platinum nanoclusters in 5 ml of deionized water and add two drops of KOH (2M). Then add the solution dropwise to the mixed solution of chloroplatinic acid hexahydrate and hydrotalcite. Impregnate and reduce for 5 h with stirring. After the reaction is complete, centrifuge and wash (twice with deionized water and twice with ethanol) at a speed of 8000-10000 rpm. After separation, place the precipitate in a vacuum drying oven at 70℃ and dry overnight. Then grind and sieve to obtain platinum nanoclusters / high-entropy hydrotalcite nanocomposite material. In this composite material, the loading of the noble metal platinum on the hydrotalcite nanosheets is 5% (molar percentage).

[0048] In this embodiment, the transmission electron microscope (TEM) image of the prepared platinum nanoclusters / high-entropy hydrotalcite nanocomposite material is shown below. Figure 1 As shown. Figure 1 The results show that the platinum nanoclusters / high-entropy layered double hydroxide nanocomposites have uniform sizes, with length and width ranging from approximately 50 to 150 nm. Figure 2 X-ray diffraction pattern of platinum nanoclusters / high-entropy hydrotalcite nanocomposite material. Figure 3 As shown, the electroparamagnetic resonance platinum nanoclusters / high-entropy hydrotalcite nanocomposite material exhibits a strong ability to generate reactive oxygen species. Figure 4 As shown, the UV platinum nanoclusters / high-entropy hydrotalcite nanocomposite material demonstrated a strong ability to generate reactive oxygen species.

[0049] L929 cells were incubated at 25 cm. 2 Incubate in cell culture flasks, then incubate cells (1×10⁻⁶). 4 L929 cells were seeded into 96-well plates at a ratio of 1 cell / well. L929 cells were exposed to a series of doses (0, 10, 20, 30, 40 μg / mL) of DMEM solution containing platinum nanoclusters / high-entropy hydrotalcite nanocomposite. After a further 24-hour incubation, a mixture of CCK-8 and DMEM (1:10) was added to each well of the 96-well plate. Cell viability was calculated as the ratio of well absorbance. Absorbance at 450 nm was measured using a ThermoMultiskan FC reader. Figure 5 The L929 cell compatibility diagram of the platinum nanoclusters / high-entropy hydrotalcite nanocomposite material synthesized in Example 1 is shown.

[0050] Figure 6 The image shows the nuclear magnetic resonance imaging (NMR) result of the platinum nanoclusters / high-entropy hydrotalcite nanocomposite material synthesized in Example 1.

[0051] Example 2

[0052] The preparation steps of a platinum nanoclusters / high-entropy hydrotalcite nanocomposite material are as follows:

[0053] 1) Dissolve 0.79g manganese chloride tetrahydrate, 1.62g ferric nitrate nonahydrate, 1.16g cobalt nitrate hexahydrate, 1.16g nickel nitrate hexahydrate, and 0.97g copper nitrate trihydrate in 100mL of water and mix well to obtain solution A; dissolve 1.70g sodium nitrate in a mixture containing 25mL amide and 75mL water and mix well to obtain solution B; dissolve 2.0g sodium hydroxide in 100mL of water and mix well to obtain solution C; under stirring in an 80℃ water bath, slowly add solutions A and C to solution B simultaneously, adjust the pH to 9, react for 0.5h, centrifuge, wash, freeze-dry, and then disperse in water to obtain hydrotalcite nanosheet colloids;

[0054] 2) Take 1g of the high-entropy hydrotalcite obtained in step 1) and dissolve it in 50ml of deionized water, stirring mechanically for about 30min; dissolve 0.1110g of chloroplatinic acid hexahydrate in 20ml of deionized water, then add the dissolved chloroplatinic acid hexahydrate solution to the uniformly dispersed hydrotalcite nanosheet colloid, and continue stirring for 1h; then add 0.745g of sodium borohydride (nNaBH4=40nPt) 2+Dissolve the platinum nanoclusters in 5 ml of deionized water and add two drops of KOH (2M). Then add the solution dropwise to the mixed solution of chloroplatinic acid hexahydrate and hydrotalcite. Impregnate and reduce for 5 h with stirring. After the reaction is complete, centrifuge and wash (twice with deionized water and twice with ethanol) at a speed of 8000-10000 rpm. After separation, place the precipitate in a vacuum drying oven at 70℃ and dry overnight. Then grind and sieve to obtain platinum nanoclusters / high-entropy hydrotalcite nanocomposite material, wherein the loading of noble metal platinum on hydrotalcite nanosheets is 5% (molar percentage).

[0055] The resulting platinum nanoclusters / high-entropy hydrotalcite nanocomposite material has nanosheets with lengths and widths of approximately 50-150 nm. When dispersed in water, the composite material exhibits excellent ROS generation performance and NMR imaging capabilities.

[0056] Example 3

[0057] The preparation steps of a platinum nanoclusters / high-entropy hydrotalcite nanocomposite material are as follows:

[0058] 1) Dissolve 0.79g manganese chloride tetrahydrate, 1.62g ferric nitrate nonahydrate, 1.16g cobalt nitrate hexahydrate, 1.16g nickel nitrate hexahydrate, and 0.97g copper nitrate trihydrate in 100mL of water and mix well to obtain solution A; dissolve 1.70g sodium nitrate in a mixture containing 25mL formamide and 75mL water and mix well to obtain solution B; dissolve 2.0g sodium hydroxide in 100mL of water and mix well to obtain solution C; under stirring in a 50℃ water bath, slowly add solutions A and C to solution B simultaneously, adjust the pH to 9, react for 3h, centrifuge, wash, freeze dry, and then disperse in water to obtain hydrotalcite nanosheet colloids;

[0059] 2) Take 1g of the high-entropy hydrotalcite obtained in step 1) and dissolve it in 50ml of deionized water, stirring mechanically for about 30min; dissolve 0.1110g of chloroplatinic acid hexahydrate in 20ml of deionized water, then add the dissolved chloroplatinic acid hexahydrate solution to the uniformly dispersed hydrotalcite nanosheet colloid, and continue stirring for 1h; then add 0.745g of sodium borohydride (nNaBH4=40nPt) 2+ Dissolve the platinum nanoclusters in 5 ml of deionized water and add two drops of KOH (2M). Then add the solution dropwise to the mixed solution of chloroplatinic acid hexahydrate and hydrotalcite. Impregnate and reduce for 5 h with stirring. After the reaction is complete, centrifuge and wash (twice with deionized water and twice with ethanol) at a speed of 8000-10000 rpm. After separation, place the precipitate in a vacuum drying oven at 70℃ and dry overnight. Then grind and sieve to obtain platinum nanoclusters / high-entropy hydrotalcite nanocomposite material, wherein the loading of noble metal platinum on hydrotalcite nanosheets is 5% (molar percentage).

[0060] The resulting platinum nanoclusters / high-entropy hydrotalcite nanocomposite material has nanosheets with lengths and widths of approximately 50-150 nm. When dispersed in water, the composite material exhibits excellent ROS generation performance and NMR imaging capabilities.

[0061] Example 4

[0062] Example 1 was repeated, except that in step 1), "dissolving 1.70g of sodium nitrate in a mixture containing 25mL of polyethylene glycol 400 and 75ml of water" was replaced with "dissolving 1.70g of sodium nitrate in a mixture containing 20mL of polyethylene glycol 400 and 80ml of water"; all other conditions remained unchanged, and the precursor high-entropy layered double hydroxide nanosheet material was prepared. The resulting platinum nanoclusters / high-entropy layered double hydroxide nanocomposite material had nanosheets with a length and width of approximately 50-150nm.

[0063] When dispersed in water, the composite material exhibited good ROS generation performance under nuclear magnetic resonance imaging.

[0064] Example 5

[0065] Repeat Example 1, except that in step 2), "dissolve 0.1110 g of chloroplatinic acid hexahydrate in 20 ml of deionized water" is replaced with "dissolve 0.2220 g of chloroplatinic acid hexahydrate in 20 ml of deionized water", and "dissolve 0.745 g of sodium borohydride (nNaBH4 = 40nPt)" is replaced with "dissolve 0.2220 g of chloroplatinic acid hexahydrate in 20 ml of deionized water". 2+ Replace "dissolve in 5ml of deionized water" with "dissolve 1.49g of sodium borohydride (nNaBH4=40nPt)". 2+ The platinum nanoclusters were dissolved in 5 ml of deionized water; with other conditions remaining unchanged, a platinum nanocluster / high-entropy hydrotalcite nanocomposite material with a loading of 10% (molar percentage) was prepared. The length and width of the obtained composite material were 50-150 nm.

[0066] When dispersed in water, the composite material exhibited good photoacoustic imaging performance and excellent sonodynamic therapy effects.

[0067] Example 6

[0068] Example 1 was repeated, except that in step 1), "dissolving 1.70 g of sodium nitrate in a mixture containing 25 mL of polyethylene glycol 400 and 75 mL of water" was replaced with "dissolving 0.034 g of sodium nitrate in a mixture containing 25 mL of Tween and 75 mL of water"; all other conditions remained unchanged, and the precursor high-entropy layered double hydroxide nanosheet material was prepared. The resulting platinum nanoclusters / high-entropy layered double hydroxide nanocomposite material had nanosheets with a length and width of approximately 50-150 nm. When dispersed in water, the composite material exhibited good NMR imaging capabilities and ROS generation performance.

[0069] Example 7

[0070] Example 2 was repeated, except that "stirred in an 80°C water bath at 50°C" was replaced with "stirred in a 50°C water bath at 80°C" in step 1). All other conditions remained unchanged, and the precursor high-entropy layered double hydroxide (TLD) nanosheet material was prepared. The resulting platinum nanoclusters / high-entropy TLD nanocomposite material had nanosheets with lengths and widths of approximately 50-150 nm. When dispersed in water, the composite material exhibited good ROS generation performance and NMR imaging capabilities.

[0071] Example 8

[0072] Example 2 was repeated, except that "stirred in an 80°C water bath at 70°C" in step 1) was replaced with "stirred in a 70°C water bath at 80°C". "Reaction time 0.5 h" in step 1) was replaced with "reaction time 3 h"; all other conditions remained unchanged, and high-entropy layered double hydroxide nanosheets were prepared. The resulting platinum nanoclusters / high-entropy layered double hydroxide nanocomposite material had nanosheets with lengths and widths of approximately 50-150 nm. When dispersed in water, the composite material exhibited good ROS generation performance and NMR imaging capabilities.

[0073] Example 9

[0074] Repeat Example 3, except that in step 2), "0.1110 g of chloroplatinic acid hexahydrate is dissolved in 20 ml of deionized water" is replaced with "0.4440 g of chloroplatinic acid hexahydrate is dissolved in 20 ml of deionized water", and "0.745 g of sodium borohydride (nNaBH4 = 40nPt) is dissolved in 20 ml of deionized water". 2+ Replace "dissolve in 5ml of deionized water" with "dissolve 2.98g of sodium borohydride (nNaBH4=40nPt)". 2+ The platinum nanoclusters were dissolved in 5 ml of deionized water; under otherwise unchanged conditions, a platinum nanoclusters / high-entropy layered double hydroxide nanocomposite material with a loading of 20% (molar percentage) was prepared. The obtained platinum nanoclusters / high-entropy layered double hydroxide nanocomposite material had nanosheets with a length and width of approximately 50-150 nm. When dispersed in water, the composite material exhibited good nuclear magnetic resonance imaging capabilities and ROS generation performance.

[0075] Example 10

[0076] Example 1 was repeated, except that "impregnation reduction for 5 hours" in step 2) was replaced with "impregnation reduction for 8 hours". All other conditions remained unchanged, and a platinum nanoclusters / high-entropy hydrotalcite nanocomposite material was prepared. The resulting composite material had a size of 50-150 nm. When dispersed in water, the composite material exhibited good ROS generation performance and NMR imaging capabilities.

[0077] Comparative Example 1

[0078] The preparation method is basically the same as in Example 1, except that the "mixture containing 25 mL of polyethylene glycol 400 and 75 mL of water" in 1) is replaced with "aqueous solution". The other conditions remain unchanged, but the platinum nanoclusters / high-entropy hydrotalcite nanocomposite material with a length and width of 50-150 nm cannot be obtained.

[0079] Comparative Example 2

[0080] The preparation method is basically the same as in Example 1, except that “pH is controlled at 9” in 1) is replaced with “pH is controlled at 7”, and the other conditions remain unchanged. Platinum nanoclusters / high-entropy hydrotalcite nanocomposite materials with a length and width of 50-150 nm cannot be obtained.

[0081] Comparative Example 3

[0082] The preparation method is basically the same as in Example 1, except that "stirring in an 80°C water bath" in 1) is replaced with "stirring in a 0°C water bath". The other conditions remain unchanged, but the platinum nanoclusters / high-entropy hydrotalcite nanocomposite material with a length and width of 50-150 nm cannot be obtained.

[0083] Comparative Example 4

[0084] Repeat Example 1, except that in step 1), nickel-iron hydrotalcite nanosheets are obtained, and the other conditions remain unchanged, to prepare a platinum nanocluster / nickel-iron hydrotalcite nanocomposite material.

[0085] The UV comparison images of the platinum nanoclusters / high-entropy layered double hydroxide nanocomposite synthesized in Example 1 and the platinum nanoclusters / nickel-iron layered double hydroxide nanocomposite in this comparative example show the generation of reactive oxygen species. Figure 7 As shown.

[0086] Obviously, the above embodiments of the present invention are merely examples for clearly illustrating the present invention, and are not intended to limit the implementation of the present invention. For those skilled in the art, other variations or modifications can be made based on the above description. It is impossible to exhaustively list all the implementation methods here. All obvious variations or modifications derived from the technical solutions of the present invention are still within the protection scope of the present invention.

Claims

1. A hydrotalcite nanocomposite material, characterized in that, The composite material contains a high-entropy hydrotalcite nanosheet matrix and platinum nanoclusters supported on the matrix; The cations in the layers of the high-entropy hydrotalcite nanosheet matrix include Cu, Mn, Co, Ni, and Fe cations.

2. The hydrotalcite nanocomposite material according to claim 1, characterized in that, The high-entropy hydrotalcite nanosheet matrix has a length and width of 50-150 nm and a thickness of 3-4 nm. Preferably, in the layers of the high-entropy hydrotalcite nanosheet matrix, the molar ratio of each metal cation is 1:1:1:1:

1.

3. The hydrotalcite nanocomposite material according to claim 1, characterized in that, The composite material has a length and width of 50-150 nm and a thickness of 5-7 nm. Preferably, the molar content of platinum nanoclusters in the composite material is 3-5%.

4. The method for preparing the hydrotalcite nanocomposite material according to any one of claims 1-3, characterized in that, Includes the following steps: An aqueous solution of a soluble metal salt and an aqueous solution of sodium hydroxide were simultaneously added to an aqueous solution containing a hydrotalcite growth inhibitor and sodium nitrate. The mixture was stirred at 50-100℃ for 0.5-6 hours, centrifuged, washed, and dried to obtain high-entropy copper-manganese-cobalt-nickel-iron hydrotalcite nanosheets. The high-entropy copper-manganese-cobalt-nickel-iron hydrotalcite nanosheets were dispersed in water to obtain a colloid; An aqueous solution of chloroplatinic acid hexahydrate was added to the colloid and stirred until homogeneous to obtain a mixture. An aqueous solution of sodium borohydride containing potassium hydroxide was added dropwise to the mixture, and an impregnation and reduction reaction was carried out under stirring. After centrifugation, washing, separation, and grinding of the precipitate, the hydrotalcite nanocomposite material was obtained.

5. The preparation method according to claim 4, characterized in that, The soluble salts of the metal are soluble salts of Cu, Mn, Co, Ni, and Fe. Preferably, the soluble salt of the metal is a mixture of copper nitrate, manganese chloride, cobalt nitrate, nickel nitrate, and ferric nitrate.

6. The preparation method according to claim 4, characterized in that, The volume ratio of the aqueous solution of the soluble salt of the metal to the aqueous solution of sodium hydroxide is 1:(0.8-1.5).

7. The preparation method according to claim 4, characterized in that, In the mixture containing hydrotalcite growth inhibitor and sodium nitrate, the volume ratio of hydrotalcite growth inhibitor to water is 1:3-1:4, and the concentration of sodium nitrate is 12-20 mg / mL, preferably 17 mg / mL.

8. The preparation method according to claim 4, characterized in that, The mass ratio of high-entropy copper-manganese-cobalt-nickel-iron hydrotalcite nanosheets to chloroplatinic acid hexahydrate in the colloid is 5-25%.

9. The preparation method according to claim 4, characterized in that, The impregnation reduction reaction is carried out at a temperature of 20-30℃ for 3-8 hours.

10. The application of the hydrotalcite nanocomposite material as described in any one of claims 1-3 in the preparation of nanomedicines or nuclear magnetic resonance imaging agents.

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