Randomly sample single-person multi-channel full-automatic chemiluminescence analyzer

Abstract

The application discloses a single-person multi-channel full-automatic chemiluminescence analyzer capable of random sample loading, which comprises a whole machine frame module, a plurality of groups of independent single-person card strip processing modules are arranged side by side on the whole machine frame module, and a card strip detection reading module is installed at the rear of the independent single-person card strip processing module on the whole machine frame module; the independent single-person card strip processing module comprises a card strip Y-axis reciprocating motion module and a sample adding module; the independent single-person card strip processing module in the whole machine is a standard module, the processing action flow of the single-person card strip is completed in the independent single-person card strip processing module, the actions of adjacent single-person card strip processing modules do not interfere with each other, so that the single sample can be measured as soon as it is loaded, the instrument is flexible, the requirement that the number of on-site test samples is small and the samples can be randomly and scatteredly detected is completely solved, and the reagent card strip is single-person packaging, and there is no bottle opening, expiration date limitation and reagent waste.

Description

Technical Field

[0001] This invention relates to the field of fully automated chemiluminescence immunoassay equipment for in vitro diagnostics, specifically to a multi-channel fully automated chemiluminescence analyzer capable of random sample loading for single individuals. Background Technology

[0002] Traditional chemiluminescence immunoassay analyzers are complex, bulky, and expensive, making them unsuitable for small-batch sample processing applications. In recent years, with advancements in science and technology, point-of-care testing (POCT) devices that are simple to operate and miniaturized have emerged. These devices, used in conjunction with single-use reagent strips, simplify and integrate the complex processes of large chemiluminescence analyzers into miniaturized single-use reagent strips, eliminating the need for complex liquid path systems. The use of TIP heads for sample addition avoids contamination, and the devices are easy to operate, enabling immediate and rapid test results.

[0003] For example, patent CN119224347A discloses a fully automated POCT chemiluminescence device, including: a mounting frame; a reaction chamber assembly disposed on the mounting frame, the reaction chamber assembly being movable back and forth along the length of the mounting frame, the reaction chamber assembly being used to load reagent strips; a PMT assembly disposed at the rear end of the mounting frame and above the reaction chamber assembly, the reaction chamber assembly being used to move the reagent strips to the PMT assembly, the PMT assembly being used to detect the luminescence value of the detection position of the reagent strips; and a multi-channel parallel preprocessing device disposed on the mounting frame and in front of the PMT device, the multi-channel parallel preprocessing device being located above the reaction chamber assembly and being movable back and forth along the length of the mounting frame, the multi-channel parallel preprocessing device being used to aspirate and expel samples from the reagent strips and transfer magnetic particles, the multi-channel parallel preprocessing device being movable within the mounting frame and being able to move relative to the reaction chamber assembly, thereby accelerating the preprocessing speed of the multi-channel parallel preprocessing device.

[0004] However, most existing chemiluminescence detection POCT platforms are multi-channel parallel centralized sample introduction and detection mechanisms, or dual-channel integrated mechanisms, typically with 8-12 channels. While each run can process 8-12 samples, only the same batch of reagents with the same workflow can be tested at a time. Even with dual-channel integrated mechanisms, only two different reagent workflows can be tested. When multiple different reagent workflows are required, the instrument cannot perform them simultaneously. Moreover, when the sample volume is small, either channels are idle and wasted, or new samples cannot be processed in time during instrument operation, delaying sample processing. Some POCT devices use magnetic sleeves and magnetic rods for magnetic separation technology. To avoid cross-contamination, magnetic sleeve consumables must be added to the reagent cards, increasing the cost per reagent card. Furthermore, since large-scale chemiluminescence analyzers typically use multi-person reagent kits, there is a shelf life issue after opening. If the kits are not used within the expiration date, reagents will become ineffective and wasted.

[0005] For primary hospitals with small sample sizes but a focus on sample testing flexibility and reagent costs, there is an urgent need for a testing instrument that can truly enable on-demand testing of samples, is highly flexible, has a small footprint, and has low reagent and consumable costs. Summary of the Invention

[0006] The purpose of this invention is to provide a fully automated chemiluminescence analyzer with single-person, multi-channel, random sample loading. It enables on-demand testing of samples, employs single-person reagent packaging strips and a liquid-free system, and achieves fully automated sample detection. At the same time, its sensitivity and precision are comparable to large-scale chemiluminescence detection instruments. Furthermore, due to the use of single-person reagent packaging strips, there are no issues with reagent expiration dates or reagent waste.

[0007] To achieve the above objectives, the present invention provides the following technical solution: a fully automated chemiluminescence analyzer with random sample loading for single-person use and multiple channels, comprising a frame module, on which multiple independent single-person sample strip processing modules are arranged in a parallel array, and a strip detection and reading module is installed on the frame module at the rear of the independent single-person sample strip processing modules. Each independent single-person sample strip processing module includes a strip Y-axis reciprocating motion module for sample aspiration and mixing, and a sample loading module. The strip Y-axis reciprocating motion module includes a strip injection base plate fixedly mounted on the frame module, a strip injection linear guide rail fixedly mounted on the base plate, and a drive base slidably mounted on the guide rail. A strip processing slot module for immediate testing of a single sample is fixedly mounted on the drive base. The sample loading module includes a sample loading Z-axis module mounted on the frame module, and a sample loading pump with TIP head pickup, withdrawal, and detection functions is longitudinally mounted on the Z-axis module.

[0008] The sample loading module includes a sample loading Z-axis module and a sample loading pump.

[0009] Preferably, the Y-axis reciprocating motion module of the card strip further includes a card strip feeding screw motor mechanism installed on the card strip feeding base plate, wherein the screw in the card strip feeding screw motor mechanism is threadedly connected to the drive base; a sample feeding support is vertically fixed on the card strip feeding base plate, and a motion origin sensor serving as the starting position of the reciprocating motion of the drive base is also fixed on the card strip feeding base plate.

[0010] Preferably, the card strip processing slot module includes a card strip placement slot fixed on the drive base. A TIP retraction movable core is elastically connected to the side of the card strip placement slot away from the motion origin sensor via a TIP retraction spring. The card strip placement slot is provided with a new TIP head placement position and a waste TIP head placement position. The waste TIP head placement position is located near the TIP retraction movable core, and the new TIP head placement position is located next to the waste TIP head placement position and away from the TIP retraction movable core. A reagent card strip is embedded in the card strip placement slot. A magnetic screw slide module is installed in the card strip placement slot below the reagent card strip and at a 20° angle to it. A card strip positioning check sensor is fixed to the side of the card strip placement slot near the motion origin sensor.

[0011] Preferably, the magnetic screw slide module includes a screw slide motor mechanism fitted into the card strip placement slot. A magnetic insert block is slidably mounted on the screw slide motor mechanism via a screw drive. A magnetic block is fitted and fixed on the magnetic insert block. The magnetic block tilts upward and fits against the side of the magnetic bead cleaning position in the reagent card strip to achieve the function of magnetic bead adsorption in the magnetic bead cleaning position. A control circuit board connected to the control signal line of the magnetic screw slide module is fixed on the card strip placement slot. The input and output of the control signal of the magnetic screw slide module are completed by the connection of the control circuit board.

[0012] Preferably, the card strip placement slot is further equipped with a thermally conductive incubation block and a heating film, wherein the power cord of the heating film is connected to a control circuit board, the control circuit board controls the thermally conductive incubation block and the heating film to be energized and heated, and controls the ambient temperature near the magnetic bead cleaning position in the reagent card strip; an over-temperature protection switch with heating protection function is embedded in the card strip placement slot, and a temperature sensor for detecting the temperature of the thermally conductive incubation block is installed in the card strip placement slot.

[0013] Preferably, the sample loading Z-axis module includes a sample loading Z-axis bracket fixed on a sample loading support, a sample loading linear guide rail fixed longitudinally on the sample loading Z-axis bracket, and a Z-axis slider adapter block slidably mounted on the sample loading linear guide rail; the sample loading Z-axis bracket is also equipped with a sample loading screw motor mechanism that drives the Z-axis slider adapter block to slide longitudinally on the sample loading linear guide rail, the screw in the sample loading screw motor mechanism is threadedly connected to the Z-axis slider adapter block, and the sample loading pump is fixedly mounted on the Z-axis slider adapter block; a sample loading origin sensor that senses the starting position of the Z-axis slider adapter block is fixed on the sample loading Z-axis bracket.

[0014] The sample loading Z-axis module includes a sample loading Z-axis bracket, a sample loading lead screw motor mechanism, a sample loading linear guide, a Z-axis slider adapter block, and a sample loading origin sensor.

[0015] Preferably, the card strip detection and reading module includes a detection and reading X-axis motion module installed in the overall frame module, and a detection and reading Z-axis motion module is slidably mounted on the detection and reading X-axis motion module.

[0016] The barcode detection and reading module includes an X-axis motion module for detection and reading, a Z-axis motion module for detection and reading, a photomultiplier tube, and a barcode scanner.

[0017] Preferably, the detection reading X-axis motion module includes a detection module base plate fixed in the overall frame module, an X-axis linear guide slider slidably mounted on the detection module base plate, and a detection reading Z-axis motion module fixed on the X-axis linear guide slider; an X-axis drive motor is fixed at the bottom of the detection module base plate, the output end of the X-axis drive motor is connected to a synchronous pulley, the synchronous pulley is rotatably mounted on one side of the detection module base plate, and a synchronous belt idler pulley is rotatably connected to the other side of the detection module base plate surface, with a synchronous toothed belt connecting the synchronous belt idler pulley and the synchronous pulley; an X-axis origin sensor for sensing the starting position of the X-axis linear guide slider is fixed on the side of the detection module base plate surface near the synchronous pulley.

[0018] The X-axis motion module for detecting readings includes an X-axis drive motor, a detection module base plate, an X-axis linear guide slider, a synchronous toothed belt, a synchronous pulley, a synchronous idler pulley, and an X-axis origin sensor.

[0019] Preferably, the detection reading Z-axis motion module includes a detection Z-axis bracket fixed on the X-axis linear guide slider, a Z-axis linear guide slider slidably mounted on the detection Z-axis bracket, a Z-axis lead screw motor mechanism for driving the Z-axis linear guide slider to slide longitudinally mounted on the detection Z-axis bracket, the lead screw in the Z-axis lead screw motor mechanism being threadedly connected to the Z-axis linear guide slider, and a Z-axis origin sensor for sensing the starting position of the Z-axis linear guide slider's movement mounted on the detection Z-axis bracket; a Z-axis adapter block is fixedly mounted on the Z-axis linear guide slider, a photomultiplier tube is fixed on the Z-axis adapter block via a right-angle mounting block, and a barcode scanner is mounted next to the right-angle mounting block via a mounting bracket.

[0020] The Z-axis motion module for detecting readings includes a Z-axis lead screw motor mechanism, a Z-axis linear guide slider, a Z-axis detection bracket, a Z-axis adapter block, a Z-axis origin sensor, a mounting bracket, and a right-angle mounting block.

[0021] Preferably, the overall frame module includes a device frame that protects the entire device. A host computer / industrial control integrated machine is mounted on the device frame, containing software for controlling the movement of the analyzer and displaying the operation on a monitor interface. A top-level circuit board mounting plate is mounted above the device frame, and a control program PCBA board for controlling the operation of the independent single-use card strip processing module is mounted on the top-level circuit board mounting plate. The number of control program PCBA boards matches the number of independent single-use card strip processing modules. A power filter for connecting to an external power source is also mounted on the device frame. The fuse inside the power filter protects the instrument from overload and short circuits. An instrument power supply is fixed on the device frame to convert the external voltage connected to the power filter into the operating voltage required by the instrument. A mounting base plate providing a mounting platform for the independent single-use card strip processing modules is fixed within the device frame. A TIP removal bracket and TIP removal guard are fixed on the mounting base plate to assist in removing the waste TIP heads from the independent single-use card strip processing modules. A waste TIP recycling box for uniformly collecting waste TIP heads is also fixed on the device frame.

[0022] The overall frame module includes the equipment frame, the host computer industrial control all-in-one machine, the top circuit board mounting plate, the control program PCBA board, the power filter, the instrument power supply, the mounting base plate, the TIP removal bracket, the TIP removal edge guard, and the waste TIP recycling box.

[0023] Compared with the prior art, the beneficial effects of the present invention are: it can realize random sample testing, and samples can be tested as soon as they arrive, without being limited by whether the instrument is already conducting tests, and the instrument has no liquid circuit system, and the TIP head is a disposable consumable.

[0024] The independent single-use card strip processing module can complete the sealing and puncture of reagent cards, fully automated sample addition, sample and reagent heating and incubation, magnetic bead cleaning and separation, and substrate addition in a single module.

[0025] The single-use reagent strips used in this device encapsulate all reagents within the strips, avoiding the issue of expiration dates after opening that is present in large bottles of test reagents, thus preventing reagent waste during use. Attached Figure Description

[0026] Figure 1 This is a schematic diagram of the overall structure of the present invention.

[0027] Figure 2 This is a schematic diagram of the left-side structure of the independent single-person card strip processing module of the present invention.

[0028] Figure 3 This is a schematic diagram of the right side of the independent single-person card strip processing module of the present invention.

[0029] Figure 4 This is a schematic diagram of the card strip processing slot module of the present invention.

[0030] Figure 5 This is a side view of the card strip processing slot module of the present invention.

[0031] Figure 6 This is a schematic diagram of the cross-sectional structure of the card strip placement groove of the present invention.

[0032] Figure 7 For the present invention Figure 6 Enlarged structural diagram at point B.

[0033] Figure 8 This is a schematic diagram of the thermally conductive incubation block and heating film structure of the present invention.

[0034] Figure 9 This is a schematic diagram of the sample addition module structure of the present invention.

[0035] Figure 10 This is a schematic diagram of the Z-axis module structure for sample loading in this invention.

[0036] Figure 11 This is a schematic diagram of the card strip detection and reading module of the present invention.

[0037] Figure 12 This is a schematic diagram of the X motion module for detecting readings according to the present invention.

[0038] Figure 13 This is a schematic diagram of the Z-axis motion module for detecting readings according to the present invention.

[0039] Figure 14 This is a schematic diagram of the overall frame module structure of the present invention.

[0040] In the diagram: 1. Independent single-use card strip processing module; 2. Card strip detection and reading module; 3. Overall frame module; 4. Card strip Y-axis reciprocating motion module; 5. Sample addition module; 6. Card strip injection screw motor mechanism; 7. Card strip injection base plate; 8. Card strip injection linear guide rail; 9. Drive base; 10. Card strip processing slot module; 10.1 Card strip placement slot; 10.2 TIP retraction movable core; 10.3 TIP retraction spring; 10.4 New TIP head placement position; 10.5 Reagent card strip; 10.6 Magnetic screw slide mold 10.6.1 Screw slide motor mechanism; 10.6.2 Magnet embedding block; 10.6.3 Magnet block; 10.7 Thermally conductive incubation block; 10.8 Over-temperature protection switch; 10.9 Clip positioning sensor; 10.10 Control circuit board; 10.11 Waste TIP head placement position; 10.12 Temperature sensor; 10.13 Heating film; 11 Sample dispensing support; 12 Motion origin sensor; 13 Sample dispensing pump; 14 Sample dispensing Z-axis module; 14.1 Sample dispensing Z-axis bracket; 14.2 14.3. Sample feeding lead screw motor mechanism; 14.4. Sample feeding linear guide rail; 14.5. Sample feeding origin sensor; 15.6. Detection reading X-axis motion module; 15.1. X-axis drive motor; 15.2. Detection module base plate; 15.3. X-axis linear guide rail slider; 15.4. Synchronous toothed belt; 15.5. Synchronous pulley; 15.6. Synchronous belt idler pulley; 15.7. X-axis origin sensor; 16. Detection reading Z-axis motion module; 16.1. Z-axis lead screw motor mechanism; 16.2. Z-axis linear guide rail... 16.3 Guide rail slider; 16.4 Z-axis bracket; 16.5 Z-axis adapter block; 16.6 Z-axis origin sensor; 16.7 Mounting bracket; 16.8 Right-angle mounting block; 17 Photomultiplier tube; 18 Barcode scanner; 19 Equipment frame; 20 Host computer / industrial control integrated machine; 21 Top-level circuit board mounting plate; 22 Control program PCBA board; 23 Power filter; 24 Instrument power supply; 25 Mounting base plate; 26 TIP removal bracket; 27 TIP removal edge guard; 28 Waste TIP recycling box. Detailed Implementation

[0041] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0042] Example Please see Figures 1-14The present invention provides the following technical solution: a fully automated chemiluminescence analyzer with random sample loading for single-person use and multiple channels, comprising a frame module 3, on which multiple independent single-person sample strip processing modules 1 are arranged in a parallel array, and a strip detection and reading module 2 is installed on the frame module 3 at the rear of the independent single-person sample strip processing modules 1. The independent single-person sample strip processing module 1 includes a strip Y-axis reciprocating motion module 4 for sample aspiration and mixing, and a sample loading module 5; wherein the strip Y-axis reciprocating motion... The moving module 4 includes a card strip injection base plate 7 fixedly installed on the whole machine frame module 3. A card strip injection linear guide rail 8 is fixed on the card strip injection base plate 7, and a drive base 9 is slidably installed on the card strip injection linear guide rail 8. A card strip processing slot module 10 for realizing single sample on-demand testing is fixed on the drive base 9. The sample loading module 5 includes a sample loading Z-axis module 14 installed on the whole machine frame module 3. A sample loading pump 13 with TIP head picking, retraction, and detection functions is longitudinally and movably installed on the sample loading Z-axis module 14.

[0043] Please see Figures 2-4 The Y-axis reciprocating motion module 4 of the card strip also includes a card strip feeding screw motor mechanism 6 installed on the card strip feeding base plate 7. The screw in the card strip feeding screw motor mechanism 6 is threadedly connected to the drive base 9. A sample feeding support 11 is vertically fixed on the card strip feeding base plate 7, and a motion origin sensor 12, which serves as the starting position of the reciprocating motion of the drive base 9, is also fixed on the card strip feeding base plate 7.

[0044] In this embodiment, the motor in the running card strip feeding screw motor mechanism 6 controls the screw to rotate. Under the threaded transmission between the screw and the drive base 9, the drive base 9 is controlled to reciprocate on the card strip feeding base plate 7, thereby controlling the card strip processing groove module 10 to reciprocate along the Y-axis direction. The card strip processing groove module 10 is located below the sample feeding module 5.

[0045] Please see Figures 5-8The card strip processing slot module 10 includes a card strip placement slot 10.1 fixed on the drive base 9. The side of the card strip placement slot 10.1 away from the motion origin sensor 12 is elastically connected to a TIP retraction movable core 10.2 via a TIP retraction spring 10.3. The card strip placement slot 10.1 is provided with a new TIP head placement position 10.4 and a waste TIP head placement position 10.11, wherein the waste TIP head placement position 10.11 is located near the TIP retraction movable core 10.2. On one side, the new TIP head placement position 10.4 is located next to the waste TIP head placement position 10.11 and away from the TIP retraction active core 10.2. A reagent card strip 10.5 is fitted into the card strip placement slot 10.1. A magnetic screw slide module 10.6 is installed in the card strip placement slot 10.1 below the reagent card strip 10.5 and at a 20° angle to it. A card strip positioning check sensor 10.9 is fixed on the side of the card strip placement slot 10.1 near the motion origin sensor 12.

[0046] In this embodiment, the TIP retraction movable core 10.2 and the TIP retraction spring 10.3 are elastically coordinated. The range of motion of the TIP retraction movable core 10.2 is 0-10mm. The two TIP placement positions of the clip placement groove 10.1 are the new TIP head placement position 10.4 and the waste TIP head placement position 10.11, respectively. When it is necessary to remove the waste TIP head on the left side, the clip processing groove module 10 moves as a whole to the end away from the motion origin sensor 12. When it touches the TIP retraction stop edge... At 27 o'clock, the TIP retraction movable core 10.2 contacts and presses against the TIP retraction stop 27, causing the TIP retraction movable core 10.2 to move and compress the TIP retraction spring 10.3, so that the 10mm diameter hole at the bottom of the TIP retraction movable core 10.2 moves to align with the waste TIP head placement position 10.11 on the same axis. At this time, the waste TIP falls downward from the waste TIP head placement position 10.11 and passes through the hole at the bottom of the TIP retraction movable core 10.2 into the waste TIP recycling box 28.

[0047] Please see Figures 5-8The magnetic screw slide module 10.6 includes a screw slide motor mechanism 10.6.1 fitted into the card strip placement slot 10.1. A magnet insert block 10.6.2 is slidably mounted on the screw slide motor mechanism 10.6.1 via a screw drive. A magnet block 10.6.3 is fitted and fixed on the magnet insert block 10.6.2. The magnet block 10.6.3 tilts upward and fits against the side of the magnetic bead cleaning position in the reagent card strip 10.5 to achieve the function of magnetic bead adsorption in the magnetic bead cleaning position. A control circuit board 10.10 connected to the control signal line of the magnetic screw slide module 10.6 is fixed on the card strip placement slot 10.1. The input and output of the control signal of the magnetic screw slide module 10.6 are completed by the connection of the control circuit board 10.10. The card strip placement slot 10.1 is also equipped with a thermally conductive incubation block 10.7 and a heating film 10.13. The power cord of the heating film 10.13 is connected to the control circuit board 10.10. The control circuit board 10.10 controls the thermally conductive incubation block 10.7 and the heating film 10.13 to be energized and heated, and controls the ambient temperature near the magnetic bead cleaning position in the reagent card strip 10.5. An over-temperature protection switch 10.8 with heating protection function is embedded in the card strip placement slot 10.1, and a temperature sensor 10.12 is used to detect the temperature of the thermally conductive incubation block 10.7 in the card strip placement slot 10.1.

[0048] When the motor in the lead screw slide motor mechanism 10.6.1 drives the lead screw to rotate, under the threaded transmission between the lead screw and the magnet insert block 10.6.2, the magnet insert block 10.6.2 can be controlled to reciprocate along the inclined direction in the card strip placement groove 10.1. When the magnet block 10.6.3 is driven to the far-end set position by the lead screw slide motor mechanism 10.6.1, it is attached to the side of the magnetic bead cleaning position in the reagent card strip 10.5, thereby achieving the function of magnetic bead adsorption in the magnetic bead cleaning position.

[0049] The control circuit board 10.10 controls the heating film 10.13 and the thermally conductive incubation block 10.7 to be energized and heated, so that the ambient temperature near the magnetic bead cleaning position in the reagent strip 10.5 is heated to 37°C, so as to achieve the incubation function of reagent and sample. At the same time, the over-temperature protection switch 10.8 can play a heating protection role. When the heating control program is disordered and the thermally conductive heating film 10.13 continues to heat up, and the temperature of the thermally conductive incubation block 10.7 exceeds 50°C, the over-temperature protection switch 10.8 disconnects the heating power supply, so as to protect the entire strip placement slot 10.1 from being burned by high temperature.

[0050] Please see Figure 9 and Figure 10The sampling Z-axis module 14 includes a sampling Z-axis bracket 14.1 fixed on the sampling support 11. A sampling linear guide rail 14.3 is longitudinally fixed on the sampling Z-axis bracket 14.1. A Z-axis slider adapter block 14.4 is slidably mounted on the sampling linear guide rail 14.3. The sampling Z-axis bracket 14.1 is also equipped with a sampling screw motor mechanism 14.2 that drives the Z-axis slider adapter block 14.4 to slide longitudinally on the sampling linear guide rail 14.3. The screw in the sampling screw motor mechanism 14.2 is threadedly connected to the Z-axis slider adapter block 14.4. The sampling pump 13 is fixedly mounted on the Z-axis slider adapter block 14.4. A sampling origin sensor 14.5 that senses the starting position of the Z-axis slider adapter block 14.4 is fixed on the sampling Z-axis bracket 14.1.

[0051] When the motor in the sample feeding screw motor mechanism 14.2 drives the screw to rotate, the screw and the Z-axis slider adapter block 14.4 are driven to reciprocate along the Z-axis direction on the sample feeding linear guide rail 14.3 under the threaded transmission between the screw and the Z-axis slider adapter block 14.4, thereby adjusting the height of the sample feeding pump 13 from the card strip placement groove 10.1, so as to realize the sample aspiration, blowing and mixing and other actions.

[0052] Please see Figures 11-13 The card strip detection reading module 2 includes a detection reading X motion module 15 installed in the whole frame module 3, and a detection reading Z axis motion module 16 is slidably installed on the detection reading X motion module 15.

[0053] Please see Figure 12 The detection reading X-axis motion module 15 includes a detection module base plate 15.2 fixed in the overall frame module 3. An X-axis linear guide slider 15.3 is slidably mounted on the detection module base plate 15.2. The detection reading Z-axis motion module 16 is fixed on the X-axis linear guide slider 15.3. An X-axis drive motor 15.1 is fixed at the bottom of the detection module base plate 15.2. A synchronous pulley 15.5 is connected to the output end of the X-axis drive motor 15.1. The synchronous pulley 15.5 is rotatably mounted on one side of the detection module base plate 15.2, and a synchronous belt idler pulley 15.6 is rotatably connected to the other side of the surface of the detection module base plate 15.2. A synchronous toothed belt 15.4 is connected between the synchronous belt idler pulley 15.6 and the synchronous pulley 15.5. An X-axis origin sensor 15.7 for sensing the starting position of the X-axis linear guide slider 15.3 is fixed on the side of the detection module base plate 15.2 near the synchronous pulley 15.5.

[0054] In this embodiment, the X-axis drive motor 15.1 drives the synchronous pulley 15.5 to rotate. Under the transmission of the synchronous toothed belt 15.4, it can drive the synchronous idler pulley 15.6 on the other side to rotate. The synchronous toothed belt 15.4 is fixedly installed with the detection Z-axis bracket 16.3, and the detection Z-axis bracket 16.3 is fixedly installed on the X-axis linear guide slider 15.3. When the synchronous toothed belt 15.4 moves, it can drive the detection Z-axis bracket 16.3 to move synchronously along the X-axis direction.

[0055] Please see Figures 11-13 The detection reading Z-axis motion module 16 includes a detection Z-axis bracket 16.3 fixed on the X-axis linear guide slider 15.3, a Z-axis linear guide slider 16.2 slidably mounted on the detection Z-axis bracket 16.3, a Z-axis lead screw motor mechanism 16.1 for driving the Z-axis linear guide slider 16.2 to slide longitudinally on the detection Z-axis bracket 16.3, a lead screw in the Z-axis lead screw motor mechanism 16.1 threadedly connected to the Z-axis linear guide slider 16.2, a Z-axis origin sensor 16.5 for sensing the starting position of the Z-axis linear guide slider 16.2 is mounted on the detection Z-axis bracket 16.3; a Z-axis adapter block 16.4 is fixedly mounted on the Z-axis linear guide slider 16.2, a photomultiplier tube 17 is fixed on the Z-axis adapter block 16.4 via a right-angle mounting block 16.7, and a barcode scanner 18 is mounted next to the right-angle mounting block 16.7 via a mounting bracket 16.6.

[0056] When the motor in the Z-axis lead screw motor mechanism 16.1 drives the lead screw to rotate, the Z-axis linear guide slider 16.2 can be controlled to reciprocate along the Z-axis direction under the threaded transmission between the lead screw and the Z-axis linear guide slider 16.2. When it is necessary to make a detection reading on the substrate hole of the reagent card strip 10.5, it drives the photomultiplier tube 17 to move downward along the Z-axis to the detection reading height to detect and read the luminescence value data.

[0057] Please see Figure 14The overall frame module 3 includes a device frame 19 that protects the entire device. A host computer / industrial control integrated machine 20 is mounted on the device frame 19. The host computer / industrial control integrated machine 20 contains software for controlling the movement of the analyzer and displays the operation on a monitor interface. A top-level circuit board mounting plate 21 is mounted on top of the device frame 19. A control program PCBA board 22 is mounted on the top-level circuit board mounting plate 21 to control the operation of the independent single-person card strip processing module 1. The number of control program PCBA boards 22 matches the number of independent single-person card strip processing modules 1. The device frame 19 also has a power filter for connecting to an external power source. The internal fuse of the power filter 23 protects the instrument from overload and short circuit. The instrument power supply 24 is fixed on the equipment frame 19 to convert the external voltage connected to the power filter 23 into the operating voltage (24V, 12V, 5V) required by the instrument. The equipment frame 19 is fixed with a mounting base plate 25 that provides an installation platform for the independent single-use card strip processing module 1. The mounting base plate 25 is fixed with a TIP removal bracket 26 and a TIP removal guard 27 to assist the independent single-use card strip processing module 1 in removing waste TIP heads. The equipment frame 19 is also fixed with a waste TIP recycling box 28 for uniformly collecting waste TIP heads.

[0058] The equipment frame 19 is assembled from aluminum profiles (or can be made of sheet metal welding; here we take aluminum profiles as an example) and serves to install and fix the various modules, as well as to install and fix the outer shell of the instrument.

[0059] Based on the actual sample inspection requirements, the on-site experimental personnel select the corresponding reagent strip 10.5 and place it in any set of idle independent single-use strip processing modules 1. They manually place the matched reagent strip 10.5 into the strip placement slot 10.1. The strip placement sensor 10.9 detects an obstruction signal, indicating that the reagent strip 10.5 is in place. The experimental personnel then manually place it into the new TIP head placement position 10.4. Next, the experimental personnel manually aspirate the specified sample volume (e.g., 50 μL) and add it to the sample dispensing port at the front end of the reagent strip 10.5. The corresponding reagent process start button icon displayed on the upper-level industrial control integrated computer 20 is then activated, initiating the Y-axis reciprocating motion module of the strip. 4. First, an initialization process is performed. After resetting the origin, the reagent card strip 10.5 is moved to the barcode reading position according to the reagent flow procedure. The barcode scanner 18 in the card strip detection and reading module 2 is used to read the QR code attached to the reagent card strip 10.5 to determine and verify the reagent item to which the reagent card strip 10.5 belongs. After matching with the item to be checked, the card strip Y-axis reciprocating motion module 4 moves the reagent card strip 10.5, so that the corresponding reagent well position moves directly below the sample dispensing module 5. The card strip Y-axis reciprocating motion module 4 and the sample dispensing module 5 work together to complete the actions of taking a new TIP head, sample aspiration and mixing, reagent aspiration and mixing, cleaning solution aspiration and mixing, magnetic bead cleaning and mixing, and discarding the TIP head.

[0060] After the reagent strip 10.5 in the independent single-use strip processing module 1 has completed the experimental process and the substrate has been added, the strip Y-axis reciprocating motion module 4 moves the reagent strip 10.5 to the detection reading position. The detection reading Z-axis motion module 16 starts the reading check process. The detection reading X-axis motion module 15 in the strip detection reading module 2 drives the detection reading Z-axis motion module 16 to move it above the reagent strip 10.5 that needs to be detected. The photomultiplier tube 17 is fixedly installed on the right-angle mounting block 16.7, and the detection sensor is fixed on the photomultiplier tube 17. The optical path sleeves for reading are driven by the detection Z-axis lead screw motor mechanism 16.1. Both move downwards along the Z-axis to the detection reading height to detect and read the luminous value data. The data read by the card strip detection reading module 2 is uploaded to the operating software in the upper computer industrial control integrated computer 20 for data processing and analysis. Since multiple independent single-person card strip processing modules 1 are arranged in parallel array and move independently of each other, they do not interfere with each other during the experiment. Therefore, the needs of the laboratory sample to be tested as soon as it arrives can be fully met. The testing of newly arrived samples is not limited by the fact that the instrument has been turned on and running.

[0061] In the description of this invention, it should be noted that, unless otherwise explicitly specified and limited, the terms "connected" and "linked" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium. Those skilled in the art can understand the specific meaning of the above terms in this invention according to the specific circumstances.

[0062] Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A fully automated chemiluminescence analyzer with random sample loading for single-person use and multiple channels, comprising a mainframe module (3), characterized in that: The frame module (3) of the machine is arranged in a row with multiple independent single-person card strip processing modules (1), and the frame module (3) of the machine is equipped with a card strip detection and reading module (2) at the rear of the independent single-person card strip processing module (1). The independent single-person card strip processing module (1) includes a card strip Y-axis reciprocating motion module (4) for sample aspiration and blowing and mixing, and a sample addition module (5). The Y-axis reciprocating motion module (4) of the card strip includes a card strip injection base plate (7) fixedly installed on the whole frame module (3), a card strip injection linear guide rail (8) fixed on the card strip injection base plate (7), and a drive base (9) slidably installed on the card strip injection linear guide rail (8), and a card strip processing slot module (10) for realizing the testing of a single sample as soon as it arrives is fixed on the drive base (9). The sample loading module (5) includes a sample loading Z-axis module (14) mounted on the whole machine frame module (3). A sample loading pump (13) with TIP head picking, retraction and detection functions is longitudinally mounted on the sample loading Z-axis module (14).

2. The fully automated chemiluminescence analyzer with random sample loading for single-person use according to claim 1, characterized in that: The Y-axis reciprocating motion module (4) of the card strip also includes a card strip feeding screw motor mechanism (6) installed on the card strip feeding base plate (7), and the screw in the card strip feeding screw motor mechanism (6) is threadedly connected to the drive base (9); A sample feeding support (11) is vertically fixed on the sample feeding base plate (7), and a motion origin sensor (12) is also fixed on the sample feeding base plate (7) as the starting position of the reciprocating motion of the drive base (9).

3. The fully automated chemiluminescence analyzer with random sample loading for single-person use according to claim 1, characterized in that: The card strip processing slot module (10) includes a card strip placement slot (10.1) fixed on the drive base (9). The side of the card strip placement slot (10.1) away from the motion origin sensor (12) is elastically connected to the TIP release movable core (10.2) via a TIP release spring (10.3). The card strip placement slot (10.1) is provided with a new TIP head placement position (10.4) and a waste TIP head placement position (10.11). The waste TIP head placement position (10.11) is located on the side close to the TIP release movable core (10.2), and the new TIP head placement position (10.4) is located next to the waste TIP head placement position (10.11) and away from the TIP release movable core (10.2). A reagent card strip (10.5) is embedded in the card strip placement slot (10.1). A magnetic screw slide module (10.6) is installed in the card strip placement slot (10.1) below the reagent card strip (10.5) and at a 20° angle to it. A card placement slot (10.1) near the motion origin sensor (12) is fixed with a card placement check sensor (10.9).

4. The fully automated chemiluminescence analyzer with random sample loading for single-person use, as described in claim 3, is characterized in that: The magnetic screw slide module (10.6) includes a screw slide motor mechanism (10.6.1) fitted into the card strip placement slot (10.1). A magnetic embedding block (10.6.2) is slidably mounted on the screw slide motor mechanism (10.6.1) via a screw drive. A magnetic block (10.6.3) is fitted and fixed on the magnetic embedding block (10.6.2). The magnetic block (10.6.3) tilts upward and fits against the side of the magnetic bead cleaning position in the reagent card strip (10.5) to achieve the function of magnetic bead adsorption in the magnetic bead cleaning position. A control circuit board (10.10) is fixed on the card slot (10.1) and connected to the control signal line of the magnet screw slide module (10.6). The input and output of the control signal of the magnet screw slide module (10.6) are completed by the control circuit board (10.10).

5. The fully automated chemiluminescence analyzer with random sample loading for single-person use, as described in claim 4, is characterized in that: The card strip placement slot (10.1) is also equipped with a thermally conductive incubation block (10.7) and a heating film (10.13), wherein the power cord of the heating film (10.13) is connected to the control circuit board (10.10), and the control circuit board (10.10) controls the thermally conductive incubation block (10.7) and the heating film (10.13) to be energized and heated, and controls the ambient temperature near the magnetic bead cleaning position in the reagent card strip (10.5); An over-temperature protection switch (10.8) with heating protection function is fitted into the card strip placement slot (10.1), and a temperature sensor (10.12) for detecting the temperature of the thermally conductive incubation block (10.7) is installed in the card strip placement slot (10.1).

6. The fully automated chemiluminescence analyzer with random sample loading for single-person use according to claim 1, characterized in that: The sample loading Z-axis module (14) includes a sample loading Z-axis bracket (14.1) fixed on the sample loading support (11), a sample loading linear guide rail (14.3) is longitudinally fixed on the sample loading Z-axis bracket (14.1), and a Z-axis slider adapter block (14.4) is slidably installed on the sample loading linear guide rail (14.3). The Z-axis support for sample loading (14.1) is also equipped with a sample loading screw motor mechanism (14.2) that drives the Z-axis slider adapter block (14.4) to slide longitudinally on the sample loading linear guide rail (14.3). The screw in the sample loading screw motor mechanism (14.2) is threadedly connected to the Z-axis slider adapter block (14.4), and the sample loading pump (13) is fixedly installed on the Z-axis slider adapter block (14.4). The sample loading Z-axis bracket (14.1) is fixed with a sensor (14.5) for the starting position of the sample loading origin of the moving Z-axis slider adapter block (14.4).

7. The fully automated chemiluminescence analyzer with random sample loading for single-person use according to claim 1, characterized in that: The card bar detection reading module (2) includes a detection reading X motion module (15) installed in the whole machine frame module (3), and a detection reading Z axis motion module (16) is slidably installed on the detection reading X motion module (15).

8. A fully automated chemiluminescence analyzer with random sample loading for single-person use, as described in claim 7, characterized in that: The detection reading X motion module (15) includes a detection module base plate (15.2) fixed in the whole frame module (3), an X-axis linear guide slider (15.3) is slidably mounted on the detection module base plate (15.2), and the detection reading Z-axis motion module (16) is fixed on the X-axis linear guide slider (15.3); An X-axis drive motor (15.1) is fixed to the bottom of the detection module base plate (15.2). The output end of the X-axis drive motor (15.1) is connected to a synchronous pulley (15.5). The synchronous pulley (15.5) is rotatably mounted on one side of the detection module base plate (15.2), and a synchronous belt idler pulley (15.6) is rotatably connected to the other side of the surface of the detection module base plate (15.2). A synchronous toothed belt (15.4) is connected between the synchronous belt idler pulley (15.6) and the synchronous pulley (15.5). The X-axis origin sensor (15.7) of the X-axis linear guide slider (15.3) is fixed on the side of the detection module base plate (15.2) near the synchronous pulley (15.5).

9. A fully automated chemiluminescence analyzer with random sample loading for single-person use, as described in claim 8, characterized in that: The detection reading Z-axis motion module (16) includes a detection Z-axis bracket (16.3) fixed on the X-axis linear guide slider (15.3), a Z-axis linear guide slider (16.2) slidably mounted on the detection Z-axis bracket (16.3), a Z-axis lead screw motor mechanism (16.1) for driving the Z-axis linear guide slider (16.2) to slide longitudinally on the detection Z-axis bracket (16.3), a lead screw in the Z-axis lead screw motor mechanism (16.1) threadedly connected to the Z-axis linear guide slider (16.2), and a Z-axis origin sensor (16.5) for sensing the starting position of the Z-axis linear guide slider (16.2) motion on the detection Z-axis bracket (16.3). A Z-axis adapter block (16.4) is fixedly installed on the Z-axis linear guide slider (16.2). A photomultiplier tube (17) is fixed on the Z-axis adapter block (16.4) via a right-angle mounting block (16.7). A barcode scanner (18) is installed next to the right-angle mounting block (16.7) via a mounting bracket (16.6).

10. A fully automated chemiluminescence analyzer with random sample loading for single-person use, as described in claim 1, characterized in that: The overall frame module (3) includes a device frame (19) that protects the entire device. A host computer industrial control all-in-one machine (20) is installed on the device frame (19). The host computer industrial control all-in-one machine (20) has software installed inside to control the movement of the analyzer and displays the operation on the display interface. A top-level circuit board mounting plate (21) is installed on the top of the equipment frame (19). A control program PCBA board (22) for controlling the operation of the independent single-person card strip processing module (1) is installed on the top-level circuit board mounting plate (21). The number of control program PCBA boards (22) matches the number of independent single-person card strip processing modules (1). The equipment frame (19) is also equipped with a power filter (23) for connecting to an external power source. The fuse inside the power filter (23) protects the instrument from overload and short circuit. The equipment frame (19) is also equipped with an instrument power supply (24) that converts the external voltage connected to the power filter (23) into the operating voltage required by the instrument. The equipment frame (19) is fixed with an installation base plate (25) that provides an installation platform for the independent single-person card strip processing module (1). The installation base plate (25) is fixed with a TIP removal bracket (26) and a TIP removal guard (27) that assists the independent single-person card strip processing module (1) in removing waste TIP heads. The equipment frame (19) is also fixed with a waste TIP recycling box (28) for uniformly collecting waste TIP heads.

Images