Organic selenium-rich sustained-release agent, preparation method and application thereof

Abstract

The application provides an organic selenium-rich slow-release agent and a preparation method and application thereof, and comprises selenomethionine, a stabilizer, a cosolvent, a carrier, a secondary carrier and a solvent, and the mass ratio of the selenomethionine, the stabilizer, the cosolvent, the carrier, the secondary carrier and the solvent is 2-40:2-100:5-100:2000:20:400-1200. The organic selenium-rich slow-release agent uses selenomethionine as a core component, has a good selenium supplement effect; the selenomethionine is easy to dissolve in the solvent by adding the cosolvent, and does not appear in a massive shape; the organic selenium-rich slow-release agent is prevented from oxidation and deterioration by adding the stabilizer, and the service life of the organic selenium-rich slow-release agent is prolonged; and the hardness of the organic selenium-rich slow-release agent is improved and the slow-release effect is achieved by adding the secondary carrier.

Description

Technical Field

[0001] This invention relates to the field of selenium-enriched sustained-release agent technology, specifically to an organic selenium-enriched sustained-release agent, its preparation method, and its application. Background Technology

[0002] Selenium is an essential trace element for humans and animals, possessing important physiological functions such as antioxidation, enhancing immunity, preventing Keshan disease and Kashin-Beck disease, and antagonizing heavy metal toxicity. Selenium deficiency in the human body can lead to various diseases. However, more than 40 countries and regions worldwide suffer from soil selenium deficiency, resulting in insufficient selenium content in the food chain and low dietary selenium intake.

[0003] Currently, selenium supplements on the market mainly fall into three categories: inorganic selenium (such as sodium selenite), artificially synthesized organic selenium (such as selenium yeast), and natural plant-based active selenium. While inorganic selenium has a high absorption rate, it is highly toxic, has a narrow safety window, and low bioavailability. The selenium in products like selenium yeast exists in complex forms and contains a certain proportion of inorganic selenium, resulting in unstable quality.

[0004] Selenomethionine (Se-Met) is the main form of selenium found in plant-based foods (such as Brazil nuts and selenium-enriched rice). It can be absorbed and utilized by the human body via the methionine pathway and specifically incorporated into proteins, forming a "selenium reservoir" in the body. Its safety and bioavailability are far superior to inorganic selenium. However, directly adding selenomethionine to water results in poor solubility and stability, and it is easily oxidized and precipitated, making it difficult to directly apply to water enrichment.

[0005] Therefore, developing a slow-release agent that can stably and efficiently dissolve selenomethionine in water and be conveniently applied to daily drinking water, agriculture, and aquaculture has significant market value and scientific significance. Summary of the Invention

[0006] The technical problem to be solved by this invention is how to provide an organic selenium-enriched slow-release agent that stably dissolves selenomethionine in water.

[0007] The present invention solves the above-mentioned technical problems through the following technical means:

[0008] The first aspect of the present invention provides an organic selenium-enriched sustained-release agent, comprising selenomethionine, a stabilizer, a cosolvent, a carrier, a secondary carrier, and a solvent, wherein the mass ratio of selenomethionine, stabilizer, cosolvent, carrier, secondary carrier, and solvent is 2~40:2~100:5~100:2000:20:400~1200.

[0009] Beneficial effects: The organic selenium-enriched slow-release agent of the present invention uses selenomethionine as the core component, which has a good selenium supplementation effect; the present invention adds a solubilizer to make selenomethionine easy to dissolve in the solvent and prevents it from clumping; the present invention adds a stabilizer to prevent the organic selenium-enriched slow-release agent from oxidizing and deteriorating, thus extending the service life of the organic selenium-enriched slow-release agent; the present invention adds a secondary carrier to improve the hardness of the organic selenium-enriched slow-release agent and achieve the slow-release effect.

[0010] Preferably, the stabilizer includes one or more of vitamin C, vitamin E, and sodium citrate.

[0011] Beneficial effects: The present invention can prevent the oxidation and deterioration of selenomethionine through the use of stabilizers and can extend the shelf life of organic selenium-enriched slow-release agents.

[0012] Preferably, the co-solvent includes one or more of ethanol, sodium carbonate, sodium bicarbonate, potassium carbonate, polysorbate 80, and β-cyclodextrin.

[0013] Beneficial effects: This invention improves the solubility and dissolution rate of selenomethionine in water by using a co-solvent, preventing uneven dissolution and clumping of selenomethionine in water.

[0014] Preferably, the carrier is calcium sulfate.

[0015] Preferably, the secondary carrier is calcium carbonate.

[0016] Preferably, the solvent is water.

[0017] The second aspect of the present invention provides a method for preparing the above-mentioned organic selenium-enriched sustained-release agent, comprising the following steps: adding a stabilizer and a co-solvent to a solvent, adding selenomethionine to dissolve it under stirring, then adding a carrier, stirring evenly, and then placing it in a mold to form an organic selenium-enriched sustained-release agent.

[0018] Preferably, the selenomethionine is added in small amounts multiple times and dissolved by stirring at 25°C to 45°C.

[0019] The third aspect of this invention provides the application of the above-mentioned organic selenium-enriched slow-release agent in drinking water.

[0020] Preferably, the organic selenium-enriched slow-release agent is placed in the pipe of the water dispenser, and then water is added to obtain selenium-containing drinking water.

[0021] The fourth aspect of this invention provides the application of the above-mentioned organic selenium-enriched slow-release agent in agricultural water or livestock drinking water.

[0022] Preferably, adding organic selenium-enriched slow-release agents to agricultural irrigation water, hydroponic nutrient solutions, or livestock drinking water allows them to be absorbed by crops, plants, or livestock and converted into natural organic selenium, thereby increasing the added value of agricultural products, plants, and livestock. Attached Figure Description

[0023] Figure 1 This is the standard curve of selenomethionine concentration in this invention; Figure 2 This is a photograph of the organic selenium-enriched slow-release agent prepared in Example 1 of the present invention. Figure 3 This is a physical image of the selenomethionine solution of Comparative Example 1 of this invention; Figure 4 This is a physical image of the organic selenium-enriched slow-release agent prepared in Comparative Example 2 of this invention. Detailed Implementation

[0024] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0025] Unless otherwise specified, all test materials and reagents used in the following examples are commercially available.

[0026] Unless otherwise specified in the embodiments, the techniques or conditions described in the literature in this field or in accordance with the product manual may be followed.

[0027] Example 1 This embodiment provides an organic selenium-enriched sustained-release agent and its preparation method, specifically including the following: The organic selenium-enriched sustained-release agent comprises 10g of selenomethionine, 2g of stabilizer (sodium citrate), 5g of cosolvent (sodium carbonate), 2000g of carrier (calcium sulfate), 20g of secondary carrier (calcium carbonate), and 1000g of solvent (deionized water).

[0028] Preparation of the organic selenium-enriched slow-release agent: Add 1000g of deionized water, 5g of sodium carbonate, and 2g of sodium citrate to a 10L mechanical stirrer, and start stirring (300 rpm). Slowly add 10g of selenomethionine in small amounts several times, controlling the temperature at 35±5℃, and stir for 60 minutes until homogeneous. Continue to add 2000g of calcium sulfate and 20g of calcium carbonate, and continue stirring for another 60 minutes to mix evenly, obtaining a white viscous solid. Pour this mixture into a mold cavity to form the solid, and air-dry at room temperature to obtain the organic selenium-enriched slow-release agent, the weight of which is 22±2g. (Details follow...) Figure 1 As shown, the organic selenium-enriched slow-release agent is white in color.

[0029] Example 2 This embodiment provides an organic selenium-enriched sustained-release agent and its preparation method, specifically including the following: The organic selenium-enriched sustained-release agent comprises 2g of selenomethionine, 2g of stabilizer (sodium citrate), 10g of cosolvent (sodium carbonate), 2000g of carrier (calcium sulfate), 20g of secondary carrier (calcium carbonate), and 400g of solvent (deionized water).

[0030] The preparation of the organic selenium-enriched slow-release agent is the same as in Example 1, and will not be repeated here.

[0031] Example 3 This embodiment provides an organic selenium-enriched sustained-release agent and its preparation method, specifically including the following: The organic selenium-enriched slow-release agent comprises 40g of selenomethionine, 100g of stabilizer (sodium citrate), 100g of cosolvent (sodium carbonate), 2000g of carrier (calcium sulfate), 20g of secondary carrier (calcium carbonate), and 1200g of solvent (deionized water).

[0032] The preparation of the organic selenium-enriched slow-release agent is the same as in Example 1, and will not be repeated here.

[0033] Comparative Example 1 This comparative example provides an organic selenium-enriched slow-release agent and its preparation method. The difference between this comparative example and Example 1 is that no co-solvent is added, while all other aspects are the same as in Example 1.

[0034] This comparative example failed to prepare an organic selenium-enriched sustained-release agent, such as... Figure 3 As shown, selenomethionine did not dissolve completely and formed viscous lumps.

[0035] Comparative Example 2 This comparative example provides an organic selenium-enriched slow-release agent and its preparation method. The difference between this comparative example and Example 1 is that no stabilizer was added, while all other aspects are the same as in Example 1.

[0036] This comparative example yielded an organic selenium-enriched sustained-release agent, such as... Figure 4 As shown, the organic selenium-enriched slow-release agent is yellow in color because no stabilizer was added, causing it to oxidize and deteriorate.

[0037] Comparative Example 3 This comparative example provides an organic selenium-enriched sustained-release agent and its preparation method. The difference between this comparative example and Example 1 is that no secondary carrier calcium carbonate was added, while all other aspects are the same as in Example 1.

[0038] The organic selenium-enriched slow-release agent prepared in this comparative example has poor density, is easily broken, and has a high release rate. After 140L, the selenium content cannot be detected, and the slow-release effect is not achieved.

[0039] Comparative Example 4 This comparative example provides an organic selenium-enriched slow-release agent and its preparation method. The difference between this comparative example and Example 1 is that the amount of calcium carbonate, the secondary carrier, is replaced from 20g to 300g, while all other aspects are the same as in Example 1.

[0040] The organic selenium-enriched slow-release agent prepared in this comparative example has good compactness, is not easy to demold, and has a low selenium release amount, not exceeding 5 μg.

[0041] Application examples The organic selenium-enriched slow-release agents of Examples 1-3 and Comparative Examples 1-3 were used in drinking water. The organic selenium-enriched slow-release agents were placed in the pipes of a water dispenser, and then water was dispensed normally to obtain selenium-enriched drinking water. The selenomethionine content and selenium content in the selenium-enriched drinking water were then tested.

[0042] Detection method: First, prepare seven concentrations of selenomethionine (25 μg / L, 50 μg / L, 62.5 μg / L, 125 μg / L, 250 μg / L, 500 μg / L, and 1000 μg / L) to establish a standard curve. The standard curve is shown below. Figure 1 As shown, the standard curve is y = 2249.52966x ± 1077.66226 (r = 0.99994, r 2 =0.99989).

[0043] The test method was based on liquid chromatography-mass spectrometry (LC-MS, SCIEX Triple Quad 5500+). The selected separation column was a Waters XSelect HSS T3 column, 100 Å, 5 µm, 3 mm x 250 mm, and the mobile phase was acetonitrile and water.

[0044] (1) Three tablets of organic selenium-enriched slow-release agent prepared in Example 1 were randomly selected, and the selenomethionine content and selenium content in selenium-enriched drinking water of different volumes were tested, as shown in Table 1.

[0045] Table 1

[0046] (2) Three tablets of organic selenium-enriched slow-release agent prepared in Example 2 were randomly selected, and the selenomethionine content and selenium content in selenium-enriched drinking water of different volumes were tested, as shown in Table 2.

[0047] Table 2

[0048] (3) Three tablets of organic selenium-enriched slow-release agent prepared in Example 3 were randomly selected, and the selenomethionine content and selenium content in selenium-enriched drinking water of different volumes were tested, as shown in Table 3.

[0049] Table 3

[0050] As shown in Table 1, the mass of selenomethionine in the organic selenium-enriched slow-release agent of Example 1 is 0.5% of the mass of the carrier calcium sulfate. The organic selenium-enriched slow-release agent of Example 1 is placed in the pipe of a water dispenser, and then water is dispensed normally to obtain selenium-enriched drinking water. The selenium content in the selenium-enriched drinking water is about 10 μg / L~20 μg / L (calculated as elemental selenium), which meets the national standard for selenium content in packaged drinking water. Example 1 uses selenomethionine as the core component of selenium, which is more easily absorbed by the human body.

[0051] According to Tables 2 and 3, the mass of selenomethionine in the organic selenium-enriched slow-release agent of Example 2 is 0.1% of the mass of the carrier calcium sulfate, and the mass of selenomethionine in the organic selenium-enriched slow-release agent of Example 3 is 2% of the mass of the carrier calcium sulfate. Therefore, the selenium content in the selenium-enriched drinking water of Example 2 is lower than that of Example 1. Similarly, the selenium content in the selenium-enriched drinking water of Example 3 is higher than that of Example 1. Both Examples 2 and 3 meet the national standards for selenium content in packaged drinking water.

[0052] (4) Three tablets of organic selenium-enriched slow-release agent prepared by comparative example 3 were randomly selected, and the selenomethionine content and selenium content in selenium-enriched drinking water of different volumes were tested, as shown in Table 5.

[0053] Table 4

[0054] As shown in Table 4, the organic selenium-enriched slow-release agent in Comparative Example 3 did not contain calcium carbonate as a secondary carrier, which resulted in the selenium content being undetectable in the later stages of the selenium-enriched drinking water. Therefore, the organic selenium-enriched slow-release agent had a poor effect.

[0055] (5) Three tablets of organic selenium-enriched slow-release agent prepared by comparative example 4 were randomly selected, and the selenomethionine content and selenium content in selenium-enriched drinking water of different volumes were tested, as shown in Table 5.

[0056] Table 5

[0057] As shown in Table 5, the organic selenium-enriched slow-release agent in Comparative Example 4 contained too much calcium carbonate as a secondary carrier, resulting in a low selenium content in the selenium-enriched drinking water, not exceeding 5 μg.

[0058] (6) Five random samples of selenium-enriched drinking water from Example 1 were taken and tested for possible changes in water quality standards using the National Standard for Drinking Water Quality (GB 5749-2022) as the evaluation standard. The results are shown in Table 6 below.

[0059] Table 6

[0060] As shown in Table 6, the selenium-enriched drinking water in Example 1 meets the hygiene standards for drinking water and is safe for direct consumption by humans.

[0061] The above embodiments are only used to illustrate the technical solutions of the present invention, and are not intended to limit it. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. An organic selenium-enriched sustained-release agent, characterized in that, It includes selenomethionine, stabilizer, cosolvent, carrier, secondary carrier and solvent, with the mass ratio of selenomethionine, stabilizer, cosolvent, carrier, secondary carrier and solvent being 2~40:2~100:5~100:2000:20:400~1200.

2. The organic selenium-enriched sustained-release agent according to claim 1, characterized in that, Stabilizers include one or more of vitamin C, vitamin E, and sodium citrate.

3. The organic selenium-enriched slow-release agent according to claim 1, characterized in that, Cosolvents include one or more of ethanol, sodium carbonate, sodium bicarbonate, potassium carbonate, polysorbate 80, and β-cyclodextrin.

4. The organic selenium-enriched sustained-release agent according to claim 1, characterized in that, The carrier is calcium sulfate.

5. The organic selenium-enriched sustained-release agent according to claim 1, characterized in that, The secondary carrier is calcium carbonate.

6. The organic selenium-enriched sustained-release agent according to claim 1, characterized in that, The solvent is water.

7. A method for preparing an organic selenium-enriched sustained-release agent as described in any one of claims 1-6, characterized in that, Includes the following steps: Stabilizers and co-solvents are added to the solvent, and selenomethionine is added to dissolve it under stirring. Then, carriers and secondary carriers are added, and the mixture is stirred evenly and placed in a mold to form an organic selenium-enriched sustained-release agent.

8. The method for preparing the organic selenium-enriched sustained-release agent according to claim 7, characterized in that, Selenomethionine was added in small amounts several times and stirred and dissolved at 25℃~45℃.

9. The application of an organic selenium-enriched slow-release agent as described in any one of claims 1-6 in drinking water.

10. The application of an organic selenium-enriched slow-release agent as described in any one of claims 1-6 in agricultural water or livestock drinking water.

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