Intelligent stem cell cryopreservation device

Abstract

This invention relates to the field of medical device technology, specifically to an intelligent stem cell cryopreservation device, comprising a housing with several cryopreservation chambers arranged from top to bottom inside the housing. A temperature control component is installed inside the housing. A turntable-shaped hanging frame is installed within each cryopreservation chamber, and the hanging frame is equipped with a rotation drive component connected to a controller. Several hanging slots are evenly distributed along the circumference of the hanging frame, each slot containing a sample storage unit. A pose acquisition component is installed on the hanging frame, and each hanging slot contains a placement acquisition component. A sampling drawer and a sample storage drawer are respectively located on both sides of the cryopreservation chamber. This invention achieves precise positioning and selective retrieval of samples within the housing through suspended rotational preservation, integrated suspended positioning acquisition, and pull-out sample storage, reducing the interference caused to the preservation environment of non-target samples by the need to open the entire housing for sample retrieval in traditional cryopreservation devices.

Description

Technical Field

[0001] This invention relates to the field of medical device technology, and more specifically to an intelligent stem cell cryopreservation device. Background Technology

[0002] Stem cell cryopreservation devices are specialized equipment used to maintain the long-term viability of stem cells in a deep cryogenic environment. By strictly controlling the cooling rate and storage temperature, the devices prevent cells from dying due to ice crystal damage during the freezing process.

[0003] In the prior art, for example, Chinese patent with publication number CN102599146A, a stem cell cryopreservation box and cryopreservation method are proposed. The cryopreservation box includes a box body, a box lid, and partitions. The box body has an opening at the top, and pressure regulating and fixing devices are provided on the outer sides of two opposite side walls of the box body. Multiple collection bags and multiple partitions are alternately placed in the box body, with the partitions at the top. The box lid is located at the top of the box body, and the box lid is connected to the pressure regulating and fixing devices on the side walls of the box body by a snap fastener.

[0004] In practical clinical applications, existing cryopreservation boxes typically store multiple patients' individual cryopreservation samples simultaneously within the same stem cell cryopreservation device. Because current devices often employ a disordered stacking method, and the timing of sample retrieval cannot be pre-set, operators must manually open the entire box to select the target cryopreservation bag from among numerous samples. This retrieval mechanism inevitably exposes non-target samples to the external environment, affecting the preservation of other non-target samples. Furthermore, frequent retrieval operations exacerbate disturbances to the temperature field within the box, posing a potential threat to the long-term cryopreservation stability of unretrieved samples. Therefore, it is necessary to propose an intelligent stem cell cryopreservation device that reduces interference with the preservation environment of other samples within the box during retrieval. Summary of the Invention

[0005] To address the aforementioned issues, this invention provides an intelligent stem cell cryopreservation device. By suspending and rotating the sample for preservation, and integrating suspension positioning and acquisition, along with pull-out sample storage and retrieval, it achieves precise positioning and selective storage and retrieval of the sample within the chamber, reducing the interference caused to the preservation environment of non-target samples by the need to open the entire cryopreservation device to locate the sample.

[0006] To achieve the above objectives, the technical solution of the present invention is as follows: An intelligent stem cell cryopreservation device includes a box, the inside of which includes a plurality of cryopreservation chambers from top to bottom. The box is provided with a temperature control component for regulating the temperature inside the box. The cryopreservation chambers are provided with a turntable-shaped hanging frame. The hanging frame is equipped with a rotation drive component, and the rotation drive component is signal-connected to a controller.

[0007] The hanging frame has several hanging slots evenly distributed along its circumference. Each hanging slot has a sample storage unit. The hanging frame is equipped with a pose acquisition component that is connected to the controller signal. The pose acquisition component is used to acquire the pose information of the hanging frame. The controller identifies the position angle of each hanging slot on the hanging frame based on the pose information. Each hanging slot is equipped with a placement acquisition component, which is used to identify the placement information of the sampling unit in each hanging slot.

[0008] The cryopreservation chamber is equipped with a sampling drawer and a storage drawer that are slidably connected to the chamber body on both sides. The sampling drawer is equipped with a sampling component for removing the storage unit from the hanging rack.

[0009] When the user needs to remove the target sample storage unit, the controller drives the rotating drive component to adjust the rotation angle of the hanging frame according to the placement information of each sample storage unit and the position angle of each hanging slot, so that the user can pull the sampling component to remove the target sample storage unit.

[0010] The sample drawer is equipped with a sample storage component, which is used to fill and hang the sample units to be stored on the hanging rack.

[0011] The technical principle of the above solution is as follows: This solution sets up a turntable-shaped hanging frame inside the cryogenic storage chamber of the box, and evenly opens several hanging slots along the circumference of the hanging frame for hanging sample storage units. At the same time, a pose acquisition component is configured to acquire the pose information of the hanging frame in real time to identify the position angle of each hanging slot. In conjunction with the acquisition component placed in each hanging slot, the occupancy status of each slot is monitored. When the user needs to retrieve the target sample storage unit, the controller drives the rotating drive component to rotate the hanging frame according to the association mapping between the stored sample storage unit information and the hanging slot, combined with the pose information, so that the target hanging slot is accurately aligned with the location of the sampling component on the sampling drawer, and the sampling component completes the selective directional retrieval. When the user needs to store the sample storage unit, the sample storage component automatically fills the sample storage unit into the corresponding hanging slot, thereby realizing the precise positioning and selective storage and retrieval of the sample storage unit in a closed environment.

[0012] The above approach has the following beneficial effects:

[0013] 1. This solution uses the circumferential hanging structure of the hanging frame in conjunction with the rotation drive, combined with the pose acquisition component and the placement acquisition component to locate and identify each sample storage unit, so as to achieve independent positioning and selective storage of the sample storage unit. This solves the problem that non-target samples are exposed to the external environment due to the overall opening of the cover to search for the target sample in the traditional method, and reduces the interference with the preservation effect of non-target samples.

[0014] 2. This solution utilizes a controller to combine positional information and sample storage unit placement information within the frozen environment inside the chamber to adjust the rotation of the hanging rack. This ensures that storage and retrieval operations only involve the target slot, while the rest of the chamber remains closed. This reduces the risk of increased temperature disturbances within the chamber due to higher storage and retrieval frequency, and guarantees the long-term cryopreservation stability of unused samples.

[0015] 3. This solution evenly hangs the sample storage units circumferentially on a turntable-shaped support frame. Compared to disorderly stacking, this achieves an orderly spatial arrangement and efficient utilization of the sample storage units, facilitating sample management and traceability. Furthermore, the rotation of the support frame provides a shaking motion during selection, improving the uniformity of freezing compared to conventional static placement freezing.

[0016] Furthermore, the hanging frame includes two hanging plates and a driven rod. The two ends of the driven rod are fixedly connected to the hanging plates on both sides, and the hanging plates are rotatably connected to the side walls of the corresponding cryopreservation chambers. The hanging slots are evenly opened on the hanging plates.

[0017] Each of the hanging trays is equipped with a track ring fixedly connected to the inner wall of the box. The two ends of the sample storage unit are respectively mounted in the hanging slots on their respective sides, and the track ring limits the radial displacement of the sample storage unit, so that each sample storage unit is suspended in the cryopreservation chamber.

[0018] Beneficial effects: By mounting the sample storage units at both ends in the hanging slots of the two side hanging trays and limiting them with track rings, the sample storage units are suspended. This ensures that both sides of the bag of each sample storage unit are in full contact with the cold airflow, increasing the effective heat exchange area of ​​the frozen storage bag per unit volume. At the same time, the track rings limit the radial displacement of the hanging rods, ensuring the stable suspension of the sample storage units during the rotation of the hanging trays and preventing them from colliding or falling off.

[0019] Furthermore, the rotary drive adopts a stepper motor type rotary drive, and the output shaft of the rotary drive passes through the side wall of the housing and is coaxially and fixedly connected to the driven rod.

[0020] Beneficial effects: By using a stepper motor as the rotary drive component, and leveraging the stepper motor's combined functions of step-type drive and rotation information recording, precise control and real-time feedback of the rotation angle of the hanging frame can be achieved. This enables the controller to accurately drive the hanging frame to rotate to the target angle based on the data from the pose acquisition component, ensuring that the hanging slot is accurately aligned with the sampling port or storage port, providing a reliable power and control foundation for the accurate storage and retrieval of samples.

[0021] Furthermore, the sampling component includes symmetrical sampling hooks and a first sampling rail. The sampling hooks are integrally formed on the sampling drawer, and the first sampling rail is opened on the side wall of the sampling drawer. A sampling port is opened on the rail ring near the sampling hook.

[0022] Beneficial effects: By combining the sampling hook with the first transport rail and the sampling port opened on the track ring, a mechanical path for directional, fixed-point, and fixed-angle retrieval is constructed; when the user pulls out the sampling drawer, the sampling hook and the expansion block fit together and push it to slide out along the horizontal groove. Then the expansion block slides along the first transport rail to the bottom of the sampling drawer, realizing the smooth transfer of the sample storage unit from the holding groove to the sampling drawer, simplifying the retrieval operation steps, and reducing the operational risks and cold exposure of manual direct access to the cryopreservation chamber for sampling.

[0023] Furthermore, the sample storage assembly includes two sample storage arms symmetrically hinged to the side wall of the sample storage drawer. A second sample transport rail is provided on the sample storage arm, and a sample storage port is provided on the rail ring near the sample storage arm. A sample baffle is hinged to the end of the sample storage arm near the sample storage port.

[0024] Beneficial effects: By combining the sample storage arm and the second transport rail, along with the sample storage opening on the track ring, a mechanism for the temporary storage and flexible automatic filling of sample units is constructed. The sample unit slides along the second transport rail to the baffle plate and is temporarily intercepted and stored. When the hanging frame rotates the empty hanging slot to the sample storage opening, the empty slot pushes the baffle plate open, allowing the unit to slide in. This achieves automatic, filling-type storage of sample units into empty slots, eliminating the need for manual alignment and improving storage efficiency and accuracy. Furthermore, the presence of sample units waiting to slide in serves as a pre-freezing mechanism for the sample units to be stored.

[0025] Furthermore, the hinges between the sample storage arm and the side wall of the sample storage drawer, as well as the hinges between the sample baffle and the sample storage arm, are all torsion spring type elastic hinges.

[0026] Beneficial effects: The torsion spring type elastic hinge allows the sample storage arm to swing elastically when subjected to external force and automatically reset after the external force is removed. The sample baffle automatically closes to intercept the next sample unit after completing one filling. This realizes the automatic and continuous filling process of the sample unit. At the same time, when the circular groove is full, the sample storage arm can elastically avoid mechanical jamming and ensure the reliable operation of the automatic storage mechanism.

[0027] Furthermore, the controller signal is connected to a human-machine interface system fixedly connected to the outer wall of the enclosure. The human-machine interface system is used for users to select the target sample unit to be retrieved and to enter the information of the sample unit to be stored.

[0028] Beneficial effects: The human-computer interaction system is designed to allow users to select the target sample storage unit to be retrieved or enter the information of the sample storage unit to be stored, thus constructing a human-computer interaction interface; users can complete the input of sample storage and retrieval commands without directly operating mechanical parts, realizing the informatization and intelligentization of sample management, and facilitating sample information traceability and inventory management.

[0029] Furthermore, the pose acquisition component also includes a tilt sensor fixedly connected to the hanging plate, and the placement acquisition component includes a piezoelectric sensor fixedly connected to the hanging slot.

[0030] Beneficial effects: An inclination sensor is used as a pose acquisition component fixed on the hanging plate to collect the rotation angle information of the hanging plate in real time. At the same time, a piezoelectric sensor is used as a placement acquisition component fixed on the inner side wall of the hanging slot to detect the pressure change of the hanging rod expansion block in real time to determine the occupancy status of the slot. This enables the controller to dynamically obtain the real-time position angle and occupancy status of each hanging slot, providing reliable data support for accurately controlling the rotation angle of the rotating drive component and realizing the one-to-one mapping between the information of the sample storage unit and the physical position.

[0031] Furthermore, a drainage cavity is provided at the top of the chamber, and symmetrical airflow channels are connected to both sides of the drainage cavity. A guide fan is provided on the drainage cavity to guide the flow direction of gas inside the chamber under negative pressure.

[0032] Beneficial effects: The design of the drainage cavity combined with the guide fan, along with the airflow channels connected on both sides, forms a negative pressure guidance path from bottom to top, so that the low-temperature gas in the chamber forms a circulating airflow throughout the whole; it improves the temperature stratification phenomenon that naturally deposits at the bottom of the chamber due to the difference in cold gas density, enhances the uniformity of temperature distribution in each cryopreservation chamber, and provides a more stable temperature environment for stem cell cryopreservation.

[0033] Furthermore, the airflow channel includes several fixed guide plates and movable guide plates. The fixed guide plates are all welded to the inner side wall of the box, and the movable guide plates are respectively welded to the side wall of the corresponding sampling drawer or storage drawer.

[0034] Beneficial effects: The airflow channel consists of two parts: a fixed guide plate and a movable guide plate. When the drawer (sampling drawer or retrieval drawer) is closed, the fixed guide plate and the movable guide plate together form a complete airflow guiding channel, maintaining the integrity of the circulating airflow path. When the drawer is opened, the movable guide plate moves out with the drawer, and a gap is formed between adjacent fixed guide plates. This forms a top-down air curtain barrier at the front edge of the opening, effectively reducing the direct convection between the outside humid and hot air and the environment inside the chamber when the sampling drawer or retrieval drawer is open, and reducing temperature fluctuations caused by cold air overflow and hot air intrusion.

[0035] Additional aspects and advantages of the invention will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention. Attached Figure Description

[0036] Figure 1 This is a schematic diagram of the overall structure of an embodiment of the intelligent stem cell cryopreservation device of the present invention;

[0037] Figure 2This is an axonometric sectional view of the interior of the box in an embodiment of the intelligent stem cell cryopreservation device of the present invention.

[0038] Figure 3 This is a schematic diagram showing the connection between the sampling tray and the storage tray and the hanging rack in an embodiment of the intelligent stem cell cryopreservation device of the present invention.

[0039] Figure 4 This is a schematic diagram illustrating the pulling out of the sampling drawer for sampling in an embodiment of the intelligent stem cell cryopreservation device of the present invention;

[0040] Figure 5 This is an isometric view of the hanging frame in an embodiment of the intelligent stem cell cryopreservation device of the present invention;

[0041] Figure 6 This is a cross-sectional view showing the arrangement of the drainage chamber and airflow channel in an embodiment of the intelligent stem cell cryopreservation device of the present invention.

[0042] The reference numerals in the accompanying drawings include: 1. Box body; 101. Cryogenic chamber; 2. Nitrogen storage tank; 3. Air compressor; 4. Hanging bracket; 401. Hanging plate; 402. Driven rod; 403. Hanging groove; 4031. Circular groove; 4032. Horizontal groove; 404. Track ring; 4041. Sampling port; 4042. Sample storage port; 405. Tilt sensor; 5. Sample storage unit; 501. 502. Hanging rod; 503. Frozen storage bag; 504. Expanding block; 6. Rotary drive component; 7. Sampling drawer; 705. Sampling hook; 706. First sample transport rail; 8. Sample storage drawer; 807. Sample storage arm; 808. Second sample transport rail; 809. Sample baffle; 9. Drainage chamber; 10. Guide fan; 11. Airflow channel; 1101. Fixed guide plate; 1102. Moving guide plate; 12. Human-machine interaction system. Detailed Implementation

[0043] The technical solution of the present invention will now be clearly and completely described with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0044] In the description of this invention, it should be noted that the terms "center," "upper," "lower," "left," "right," "vertical," "horizontal," "inner," and "outer," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are used only for the convenience of describing the invention and for simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on the invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0045] In the description of this invention, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "linking" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this invention based on the specific circumstances.

[0046] The following detailed description illustrates the specific implementation method:

[0047] Example 1:

[0048] This embodiment provides an intelligent stem cell cryopreservation device, specifically as follows: Figure 1 As shown, the device includes a housing 1, which contains a temperature control component for regulating its internal temperature. The temperature control component includes a nitrogen storage tank 2, an air compressor 3, and a temperature sensor (not shown in the figure). The nitrogen storage tank 2 is fixedly connected to the outer wall of the housing 1 by bolts. The input and output ends of the air compressor 3 are respectively connected to the nitrogen storage tank 2 and the interior of the housing 1. The temperature sensor is fixedly connected to the interior of the housing 1 by screws, and the temperature sensor signal is connected to a controller. The controller drives the air compressor 3 to pump liquid nitrogen from the nitrogen storage tank 2 into the housing 1 based on the real-time temperature acquisition signal from the temperature sensor, so as to maintain the low-temperature environment for cryopreserved stem cells in the housing 1.

[0049] Specifically, the housing 1 includes several cryopreservation chambers 101 arranged from top to bottom. This embodiment uses two cryopreservation chambers 101 as an example for explanation. Each cryopreservation chamber 101 serves as a cryopreservation layer unit. Taking one cryopreservation chamber 101 as an example... Figure 2 Explanation:

[0050] Combination Figure 1 and Figure 2The cryopreservation chamber 101 is provided with a turntable-shaped hanging frame 4. Several sample storage units 5 are evenly hung on the hanging frame 4 along its circumference. Each sample storage unit 5 includes a hanging rod 501 and a cryopreservation bag 502 that is snapped onto the hanging rod 501. The hanging frame 4 includes two hanging plates 401 and a driven rod 402. The two ends of the driven rod 402 are coaxially welded to the hanging plates 401 on both sides. The hanging plates 401 are rotatably connected to the side wall of the cryopreservation chamber 101 through bearings. Several hanging slots 403 are opened on the hanging plates 401 along their circumference. In this embodiment, five equidistant hanging slots 403 are opened on the hanging plate 401 as an example, that is, the angle between the line connecting the center of the adjacent hanging slot 403 and the center of the hanging plate 401 is 72°.

[0051] Each of the hanging trays 401 is equipped with a track ring 404 fixedly connected to the inner wall of the housing 1. The two ends of the hanging rod 501 are respectively mounted in the corresponding hanging slots 403 on both sides, and the track rings 404 limit the radial displacement of the sample storage unit 5 hanging on the hanging tray 401. Compared with the traditional method of stacking cryopreservation bags 502 in the cryopreservation equipment, by using the hanging rod 501 and limiting it with the hanging slots 403 and the track rings 404, the sample storage unit 5 is hung in the circumference of the hanging tray 401 in the cryopreservation chamber 101; this allows both sides of the cryopreservation bag 502 in each sample storage unit 5 to come into contact with the cold airflow, increasing the effective heat exchange area of ​​the cryopreservation bag 502 per unit volume.

[0052] In addition, because the surface temperature of the cryopreservation bags in the deep low temperature environment is much lower than the dew point of the air inside the box, water vapor in the air condenses into frost on the surface of the bags, or ice forms after a small amount of moisture seeps out from the surface of the bags during long-term cryopreservation; based on the hanging storage method, the cryopreservation bags do not come into contact with each other, which can effectively reduce the low-temperature adhesion defects caused by liquid precipitation between the cryopreservation bags compared with conventional stacking storage technology.

[0053] Based on the method of placing the sample storage unit 5 in a suspended manner, such as Figure 1 As shown, the side wall of the housing 1 is provided with several rotary drive components 6 corresponding to the positions of the cryopreservation chamber 101. The rotary drive component 6 includes a drive box and a stepper motor (preferably a CRK series stepper motor with rotation positioning function, that is, it has both step drive function and rotation information recording function, not shown in the figure). The drive box is fixedly connected to the outer side wall of the housing 1 by bolts, and the stepper motor is fixedly connected to the drive box by bolts. The output shaft of the stepper motor passes through the side wall of the housing 1 and is coaxially fixedly connected to the driven rod 402.

[0054] In addition, such as Figure 3As shown, in this embodiment, both ends of the hanging rod 501 are integrally formed with expansion blocks 503. The diameter of the expansion blocks 503 is larger than the diameter of the hanging rod 501. The purpose is to allow the length of the hanging rod 501 to extend to form symmetrical hanging plates 401 on both sides, providing operating space for the hanging rod 501 to be removed from and hung on the hanging plates 401; at the same time, combined with Figure 5 As shown, the hanging groove 403 includes a circular groove 4031 and a horizontal groove 4032 that are interconnected. The horizontal groove 4032 extends in a direction perpendicular to the radius of the hanging plate 401 and is used to provide sliding guidance and accommodating space when the hanging rod 501 slides into or out of the circular groove 4031, so that the hanging rod 501 can slide into or out of the circular groove 4031 through the horizontal groove 4032, thereby realizing the quick hanging of the sample storage unit 5 on the hanging plate 401 or the removal of it from the hanging plate 401.

[0055] Regarding the access steps for sample storage unit 5, as follows: Figure 2 As shown, in this embodiment, sampling drawers 7 (corresponding to the direction of the horizontal groove 4032 away from the circular groove 4031) and storage drawers 8 (corresponding to the direction of the horizontal groove 4032 towards the circular groove 4031) are respectively provided on both sides of the cryopreservation chamber 101 and are slidably connected to the chamber body 1. Sliding blocks are welded to both sides of the sampling drawers 7 and the storage drawers 8. Slide rails corresponding to the shape of the sliding blocks are opened on the inner walls of both sides of the cryopreservation chamber 101. Specifically:

[0056] Regarding the retrieval of sample storage unit 5, combined with Figure 3 and Figure 4 As shown, the sampling drawer 7 is equipped with a sampling component for removing the corresponding sample storage unit 5 from the sampling position of the hanging rotating frame 4. The sampling component includes a symmetrical sampling hook 701 and a first sampling rail 702. The track ring 404 has a sampling port 4041 near the sampling hook 701. The sampling hook 701 is integrally formed on the sampling drawer 7 and corresponds to the shape of the expansion block 503. The first sampling rail 702 is opened on the side wall of the sampling drawer 7. When the sampling drawer 7 responds to the action of the user pulling out the sample storage unit 5 and slides horizontally out of the cryopreservation chamber 101, the sampling hook 701 is driven by the sampling drawer 7 to attach to the expansion block 503 in the circular groove 4031 near the sampling port 4041 and pushes the expansion block 503 to slide out along the horizontal groove 4032. Then the expansion block 503 slides along the first sampling rail 702 to the bottom of the sampling drawer 7 for the user to remove.

[0057] Regarding the storage of sample unit 5, in conjunction with Figure 3 and Figure 5As shown, a sample storage assembly is provided on the sample drawer 8. The sample storage assembly is used to fill and hang the sample units 5 to be stored on the hanging frame 4. The sample storage assembly includes two sample storage arms 801 symmetrically hinged to the side wall of the sample drawer 8. The sample storage arms 801 can swing on the plane of the side wall of the sample drawer 8, and the hinge between the sample storage arms 801 and the side wall of the sample drawer 8 is a torsion spring elastic hinge. A second sample transport rail 802 is provided on the sample storage arm 801 (the groove width of the second sample transport rail 802 corresponds to the diameter of the expansion block 503). A sample storage port 4042 is provided on the track ring 404 near the sample storage arm 801. One end of the port 4042 is hinged to a sample baffle 803. The hinge between the sample baffle 803 and the sample storage arm 801 is a torsion spring type elastic hinge. The initial state of the hinge between the sample baffle 803 and the sample storage arm 801 is a closed state. When the user places the expansion block 503 of the sample storage unit 5 to be stored into the second sample transport rail 802, the expansion block 503 slides along the second sample transport rail 802 to the sample baffle 803 and is intercepted by the sample baffle 803. At this time, the controller signal controls the stepper motor to drive the hanging disk 401 to rotate. The expansion block 503 at the sample baffle 803 contacts through the circular groove 4031 on the hanging disk 401. There are two situations:

[0058] When a sample storage unit 5 is already installed in the circular groove 4031, the existing expansion block 503 in the circular groove 4031 will contact the side wall of the sample storage arm 801 and push the sample storage arm 801 to swing in the direction close to the sample storage drawer 8.

[0059] When no sample storage unit 5 is hung in the circular groove 4031, the circular groove 4031 will contact the expansion block 503 of the sample storage unit 5 to be stored at the baffle plate 803, so as to drive the expansion block 503 to push the baffle plate 803. Since the baffle plate 803 is elastically hinged to the sample storage arm 801, after the current sample storage unit slides into the circular groove 4031, the baffle plate 803 will elastically return to the initial state to block the next sample storage unit; thus realizing the automatic filling of the sample storage unit 5 to be stored.

[0060] Based on the aforementioned stem cell cryopreservation methods that offer selective access, such as Figure 5As shown, in this embodiment, a pose acquisition component is integrated on one of the holding plates 401. The pose acquisition component includes an angle sensor 405 fixedly connected to the holding plate 401 by screws. Based on this, during device initialization, the position angle correspondence between each holding slot 403 and the angle sensor 405 at the reference zero position is preset (i.e., the initial distribution angle of the five holding slots 403 is preset and established in the initial state of holding the plate), and each holding slot 403 is assigned an angle identifier that uniquely corresponds to its physical position on the holding plate 401. This identifier is then matched with the slot number of the holding slot 403, and so on. The information of the subsequent storage unit 5 is bound and stored in the controller. During subsequent operation, the tilt sensor 405 continuously monitors the current rotation angle of the hanging plate 401 in real time. Based on this real-time angle data and the preset angle identifier of each hanging slot 403, the controller dynamically calculates the real-time position angle of each hanging slot 403 relative to the fixed coordinate system of the box 1 (such as the sampling port 4041 or the storage port 4042). Based on this information, the controller precisely controls the number of rotation steps and direction of the stepper motor to ensure that when the user issues a storage command, the target hanging slot 403 can be correctly rotated to the sampling position or the storage position.

[0061] In addition, the controller signal is connected to a human-machine interaction system 12 (the human-machine interaction system 12 includes a human-machine interaction screen embedded in the outer wall of the housing 1) which is fixedly connected to the outer wall of the housing 1. The human-machine interaction system 12 is used for the user to select the target sample storage unit 5 to be retrieved and to enter the information of the sample storage unit 5 to be stored (the slot number of the corresponding hanging slot 403 is associated with the identifier signal of the corresponding hanging slot 403).

[0062] Each holding slot 403 is equipped with a data acquisition component, which includes a piezoelectric sensor (not shown in the figure) attached to the inner wall of the holding slot 403. The piezoelectric sensor is associated with the angle identifier and slot number of the corresponding holding slot. When the user enters the detailed information of the sample storage unit 5 to be stored through the human-machine interaction system 12 and performs the storage operation, the sample storage unit 5 slides into the target holding slot 403 along the second sample transport track 802. When the piezoelectric sensor on the inner wall of the holding slot 403 detects the pressure change signal generated by the pressing of the expansion block 503 in real time, it transmits the signal to the controller. The controller receives the signal. Then, the information of the sample storage unit 5 entered by the user is bound and associated with the angle identifier and the corresponding slot number, and stored in the controller to complete the one-to-one mapping between the information of the sample storage unit 5 and the physical storage location. In the subsequent retrieval or management process, when the user selects the target sample storage unit 5, the controller can accurately locate its corresponding angle identifier and slot by searching the bound information record. At the same time, when the sample storage unit 5 is removed, the piezoelectric sensor detects the disappearance of pressure, and the controller automatically decouples the angle identifier from the information of the sample storage unit 5 and updates its status to empty, ensuring the real-time accuracy and traceability of the information mapping.

[0063] Example 2:

[0064] As attached Figure 6 As shown, the difference from Embodiment 1 is that a drainage cavity 9 is provided at the top of the box 1, and a guide fan 10 is provided on the drainage cavity 9. The guide fan 10 is used to blow the frozen gas in the cryopreservation chamber 101 into the drainage cavity 9. Symmetrical airflow channels 11 are also provided on the inner side wall of the box 1. The airflow channels 11 are all connected to the drainage cavity 9. The airflow channels 11 include several fixed guide plates 1101 and movable guide plates 1102. The fixed guide plates 1101 are all welded to the inner side wall of the box 1, and the movable guide plates 1102 are respectively welded to the side wall of the corresponding sampling drawer 7 or storage drawer 8. The guide fan 10 applies negative pressure to the gas in the box 1 through the drainage cavity 9, so that the low temperature gas in each cryopreservation chamber 101 is drawn into the drainage cavity 9 from bottom to top, and then flows back down through the airflow channels 11 on both sides, forming a flow that runs through the entire box 101. The circulating airflow path of the cabinet 1: When the cabinet 1 is closed, the cold air circulates along the circulating airflow path, reducing the phenomenon of natural deposition at the bottom of the cabinet 1 due to the density difference of the cold air, and improving the uniformity of temperature control in each cryogenic chamber 101; In particular, when any sample drawer 8 or sample drawer 7 is pulled open, the corresponding movable guide plate 1102 welded to its side wall moves out synchronously with the drawer, so that a gap is formed between the movable guide plate 1102 and the adjacent fixed guide plate 1101, and the original circulation path through the movable guide plate 1102 is partially blocked. The opening front edge of the sample drawer 8 or sample drawer 7 forms an air curtain barrier flowing from top to bottom, so as to reduce the direct convection between the outside hot and humid air and the internal environment of the cabinet 1, and reduce the temperature fluctuation caused by the overflow of cold air or the intrusion of hot air when the cabinet is open.

[0065] Furthermore, during sample storage, the sample storage unit 5 is selectively loaded according to the occupancy status of each circular slot 4031 on the hanging tray 401. The sample storage unit 5 to be stored will have a redundant time to stay in the second sample transport track 802 before loading. During the stay, the flowing circulating cold air pre-cools its surface to make its temperature approach the ambient temperature inside the box 1. Thus, after being hung in the circular slot 4031, the local temperature disturbance caused by the introduction of room temperature samples is reduced, and the impact on the cryopreservation stability of other stored samples is reduced.

[0066] At the same time, the purging effect of the circulating cold air can blow off the droplets formed on the surface of the cryopreservation bags at room temperature or below the freezing temperature due to the accumulation of atomized liquid.

[0067] Obviously, the above embodiments are merely illustrative examples for clear explanation and are not intended to limit the implementation. Those skilled in the art will recognize that other variations or modifications can be made based on the above description. It is neither necessary nor possible to exhaustively list all possible implementations here. However, obvious variations or modifications derived therefrom are still within the scope of protection of this invention.

Claims

1. An intelligent stem cell cryopreservation device, comprising a housing (1), wherein a plurality of cryopreservation chambers (101) are sequentially formed from top to bottom inside the housing (1), and a temperature control component for regulating the temperature inside the housing (1) is provided therein, characterized in that, The cryopreservation chamber (101) is equipped with a turntable-shaped hanging frame (4), the hanging frame (4) is equipped with a rotation drive (6), and the rotation drive (6) is connected to a controller. The hanging frame (4) has several hanging slots (403) evenly distributed around its circumference. Each hanging slot (403) is equipped with a sample storage unit (5). The hanging frame (4) is equipped with a pose acquisition component connected to the controller signal. The pose acquisition component is used to acquire the pose information of the hanging frame (4). The controller identifies the position angle of each hanging slot (403) on the hanging frame (4) based on the pose information. Each hanging slot (403) is equipped with a placement acquisition component. The placement acquisition component is used to identify the placement information of the sampling unit in each hanging slot (403). The cryopreservation chamber (101) is provided with a sampling drawer (7) and a storage drawer (8) on both sides, which are slidably connected to the box body (1). The sampling drawer (7) is provided with a sampling component for taking the storage unit (5) off the hanging rotating frame (4). When the user needs to remove the target sample unit (5), the controller drives the rotating drive (6) to adjust the rotation angle of the hanging frame (4) according to the placement information of each sample unit (5) and the position angle of each hanging slot (403), so that the user can pull the sampling component to remove the target sample unit (5). The sample drawer (8) is equipped with a sample storage component, which is used to fill and hang the sample unit (5) to be stored on the hanging rack (4).

2. The intelligent stem cell cryopreservation device according to claim 1, characterized in that, The hanging frame (4) includes two hanging plates (401) and a driven rod (402). The two ends of the driven rod (402) are fixedly connected to the hanging plates (401) on both sides respectively. The hanging plates (401) are rotatably connected to the side wall of the corresponding cryopreservation chamber (101). The hanging slots (403) are evenly opened on the hanging plates (401). The outer side of the hanging tray (401) is provided with a track ring (404) fixedly connected to the inner side wall of the box (1). The two ends of the sample storage unit (5) are respectively mounted in the hanging groove (403), and the radial displacement of the sample storage unit (5) is limited by the track ring (404), so that each sample storage unit (5) is suspended in the cryopreservation chamber (101).

3. The intelligent stem cell cryopreservation device according to claim 2, characterized in that, The rotary drive (6) adopts a stepper motor type rotary drive. The output shaft of the rotary drive (6) passes through the side wall of the housing (1) and is coaxially fixedly connected to the driven rod (402).

4. The intelligent stem cell cryopreservation device according to claim 3, characterized in that, The sampling assembly includes a symmetrical sampling hook (701) and a first sampling rail (702). The sampling hook (701) is integrally formed on the sampling drawer (7), and the first sampling rail (702) is opened on the side wall of the sampling drawer (7). A sampling port (4041) is opened on the rail ring (404) near the sampling hook (701).

5. The intelligent stem cell cryopreservation device according to claim 4, characterized in that, The sample storage assembly includes two sample storage arms (801) symmetrically hinged to the side wall of the sample storage drawer (8). A second sample transport rail (802) is provided on the sample storage arm (801). A sample storage port (4042) is provided on the track ring (404) near the sample storage arm (801). A sample baffle plate (803) is hinged to one end of the sample storage arm (801) near the sample storage port (4042).

6. The intelligent stem cell cryopreservation device according to claim 5, characterized in that, The hinge between the sample storage arm (801) and the side wall of the sample storage drawer (8) and the hinge between the sample baffle (803) and the sample storage arm (801) are both torsion spring type elastic hinges.

7. The intelligent stem cell cryopreservation device according to claim 6, characterized in that, The controller signal is connected to a human-machine interaction system (12) fixedly connected to the outer wall of the enclosure (1). The human-machine interaction system (12) is used for the user to select the target sample unit (5) to be retrieved and to enter the information of the sample unit (5) to be stored.

8. The intelligent stem cell cryopreservation device according to claim 7, characterized in that, The pose acquisition component also includes a tilt sensor (405) fixedly connected to the hanging plate (401), and a piezoelectric sensor fixedly connected to the hanging slot (403) is placed in the acquisition component.

9. The intelligent stem cell cryopreservation device according to claim 8, characterized in that, The top of the box (1) is provided with a drainage cavity (9), and the two sides of the drainage cavity (9) are connected to symmetrical airflow channels (11). The drainage cavity (9) is provided with a guide fan (10) for guiding the flow direction of gas in the box (1) under negative pressure.

10. The intelligent stem cell cryopreservation device according to claim 9, characterized in that, The airflow channel (11) includes several fixed guide plates (1101) and movable guide plates (1102). The fixed guide plates (1101) are all welded to the inner side wall of the box (1), and the movable guide plates (1102) are respectively welded to the side wall of the corresponding sampling drawer (7) or storage drawer (8).

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