Antioxidant active peptide from litsea cubeba and preparation method and application thereof

Abstract

This invention discloses an antioxidant active peptide derived from Litsea cubeba, its preparation method, and its application, relating to the field of biotechnology. The amino acid sequence of this Litsea cubeba-derived antioxidant active peptide is NPYYFWDLSF. The preparation method of this Litsea cubeba-derived antioxidant active peptide includes the following steps: S1, extracting Litsea cubeba protein from Litsea cubeba seed cake; S2, enzymatically hydrolyzing the Litsea cubeba protein using acidic protease to obtain the enzymatic hydrolysis product; S3, separating the enzymatic hydrolysis product by ultrafiltration and collecting the ultrafiltration fraction; S4, screening candidate peptides with high antioxidant activity from the peptide fragments of the ultrafiltration fraction; the application of the Litsea cubeba-derived antioxidant active peptide in the preparation of drugs or functional foods for the prevention or treatment of oxidative stress-related diseases; the NPYYFWDLSF peptide obtained by the preparation method of this application can alleviate oxidative stress damage through the PI3K-AKT signaling pathway; this application provides a theoretical basis for the high-value utilization and potential application of Litsea cubeba by-products.

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