Methods and kits for detecting methylation cycle pathway gene variants based on time-of-flight mass spectrometry platforms
By optimizing the primer design and molecular weight arrangement of the time-of-flight mass spectrometry platform, efficient, accurate, and low-cost single-tube detection of 22 SNP sites was achieved, overcoming the limitations of multiple primer design and molecular weight arrangement in existing technologies, increasing detection throughput and reducing experimental costs.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- 上海捷易医学检验实验室有限公司
- Filing Date
- 2026-02-02
- Publication Date
- 2026-06-19
AI Technical Summary
Existing time-of-flight mass spectrometry platforms have limitations in detecting gene variations in the methylation cycle pathway, including blocking restrictions, high costs, cumbersome operation, and a limited number of detection sites. They are particularly limited in multiple primer design and molecular weight arrangement, making it difficult to achieve efficient and accurate detection of multiple sites.
By optimizing primer design and molecular weight arrangement on the time-of-flight mass spectrometry platform, single-tube detection primers for multiple gene loci were designed. A systematic molecular weight arrangement analysis system was used to adjust the molecular weight of the primers to avoid peak overlap, optimize multiple reaction compatibility, and adjust the primer pool concentration through signal equalization to achieve single-tube detection of 22 SNP loci.
It achieves efficient, accurate, and low-cost single-tube detection of 22 SNP loci, improving detection throughput and reducing experimental costs. Furthermore, the self-developed software solves the problems of commercial software censorship and high costs, ensuring the flexibility and accuracy of detection.
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