A noctuid larva food attractant and a preparation method, a food attractant and application thereof

Abstract

The application discloses a kind of noctuidae larva food attractant and preparation method, food attractant and application, including volatile component 0.0028-0.0042% by weight, nutrient component 2.3-3.5%, deionized water is supplemented to 100%.The application adopts volatile component 0.0028-0.0042%, nutrient component 2.3-3.5% combination, nutrient component provides the internal motivation of larva feeding, and volatile component provides long-distance attractant signal, and the dual mechanism of "near end attraction+remote guidance" is formed by the synergistic effect of both, has significant attractant effect to cotton bollworm, pyrausta nubidalis, beet armyworm, spodoptera exigua and other various noctuidae larvae.Has broad-spectrum attractant effect to noctuidae larvae, can reduce the number of times and the amount of drug, avoids pesticide abuse, so that pests appear drug resistance.To human and livestock, natural enemies and natural environment have no toxic side effects, and are safe to environment without pollution.

Description

Technical Field

[0001] This invention relates to the field of insecticides, specifically to a feeding attractant for Noctuidae larvae and its preparation method, as well as the feeding attractant and its application. Background Technology

[0002] Noctuid moths are primarily harmful to agriculture and forestry in their larval stage. They are voracious eaters, highly resistant to pesticides, and difficult to control, leading to frequent outbreaks and significant losses to agricultural production. With increasing awareness of environmental protection and safe production, many pesticides have been banned, and strict restrictions have been placed on their dosage and frequency. Long-term, irrational use of chemical pesticides easily leads to pesticide resistance in various agricultural pests, posing a significant threat to agricultural production. Therefore, developing a feeding attractant to increase the feeding amount of noctuid moth larvae, resulting in deeper poisoning, improving the knockdown speed of the pesticide, and optimizing its insecticidal effect is crucial.

[0003] Chinese invention patent CN119867072B discloses a feeding attractant composition for codling moth larvae and its application. It uses a combination of isoquercitrin, phlorizin, cyanidin-3-O-galactoside, and delphinidin-3-O-galactose as the feeding attractant composition. The composition uses small amounts of these components, is inexpensive, and can enhance the behavioral regulation effect of the lure on larvae, reducing production costs. However, it cannot achieve feeding attraction and control of cotton bollworm, beet armyworm, and fall armyworm larvae. Chinese invention patent CN113826644B discloses an entomopathogenic nematode preparation for controlling fall armyworm, its preparation method, and its application. The components include a feeding attractant, ecdysone, and tyrosinase inhibitor, which greatly improves the biocontrol rate of entomopathogenic nematodes against fall armyworm larvae. However, it cannot directly control fall armyworm larvae and may easily have a certain impact on the ecosystem. Summary of the Invention

[0004] In order to develop a feeding attractant that increases the amount of food consumed by noctuid moth larvae, resulting in deeper poisoning of the larvae, improving the knockdown speed of the pesticide on pests, and optimizing the insecticidal effect of the pesticide, the first aspect of the present invention provides a feeding attractant for noctuid moth larvae, comprising, by weight percentage, 0.0028%-0.0042% volatile components, 2.3-3.5% nutrient components, and deionized water to make up to 100%.

[0005] This invention employs a combination of 0.0028%-0.0042% volatile components and 2.3%-3.5% nutrient components. The nutrient components provide the intrinsic motivation for larvae to feed, while the volatile components provide long-distance attraction signals. The two work synergistically to form a dual mechanism of "near-end attraction + far-end guidance." This results in a significant attraction effect on various noctuid moth larvae, including cotton bollworm, beet armyworm, and fall armyworm.

[0006] As one implementation, the nutritional components, based on the weight percentage of the attractant for Noctuidae larvae, include 2.0%-3.0% fructose, 0.2%-0.3% L-proline, and 0.1%-0.2% inositol.

[0007] In one embodiment, the nutritional components, by weight percentage of the noctuid moth larvae attractant, include 2.5% fructose, 0.25% L-proline, and 0.15% inositol.

[0008] In one embodiment, the volatile components, based on the weight percentage of the noctuid moth larvae attractant, include 0.002% to 0.003% cis-jasmone and 0.0008% to 0.0012% n-pentanol; the noctuid moth larvae attractant also includes a solubilizer.

[0009] In one embodiment, the volatile components, by weight percentage of the noctuid moth larvae feeding attractant, include 0.0025% cis-jasmone and 0.001% n-pentanol.

[0010] A second aspect of the present invention provides a method for preparing a feeding attractant for Noctuidae larvae, comprising the following steps: The volatile components are dissolved in a co-solvent to prepare a mother liquor; The nutrient components were dissolved in deionized water to prepare an aqueous solution; Adding the mother liquor to an aqueous solution yields a feeding attractant for Noctuidae larvae.

[0011] In one implementation, the co-solvent includes, but is not limited to, anhydrous ethanol. The amount of co-solvent added is based on its ability to dissolve the volatile components. Cis-jasmonic acid and n-pentanol are readily soluble in anhydrous ethanol but have relatively low solubility in water. Direct use with water can easily lead to precipitation. Dissolving them in anhydrous ethanol first and then adding them to water can, to some extent, avoid precipitation.

[0012] In one embodiment, the 0.025g cis-jasmone is dissolved in 5mL of anhydrous ethanol, and the 0.01g n-pentanol is dissolved in 3mL of anhydrous ethanol.

[0013] A third aspect of the present invention provides a feeding attractant, comprising the above-mentioned feeding attractant and agent for Noctuidae larvae.

[0014] In one embodiment, the volume-to-mass ratio of the food attractant to the drug is 15 mL: (0.9-1.1) g.

[0015] As one embodiment, the agent includes, but is not limited to, at least one of abamectin emulsifiable concentrate, chlorantraniliprole suspension concentrate, and bromonitrile dibenzoyl peroxide suspension concentrate.

[0016] In one embodiment, the agent is a purchased finished product, and the mass concentration of the finished product is 1.8-20%.

[0017] A fourth aspect of the present invention provides a method for preparing the aforementioned attractant, comprising the following steps: diluting a noctuid moth larvae attractant and mixing it with the attractant to obtain the attractant.

[0018] In one implementation, the dilution factor is 900-1200 times.

[0019] The fifth aspect of the present invention provides an application of the aforementioned attractant in the control of cotton bollworm, beet armyworm, and fall armyworm larvae.

[0020] Compared with the prior art, the present invention has the following beneficial effects: (1) The feeding attractant for noctuid moth larvae described in this invention uses a combination of 0.0028%-0.0042% volatile components and 2.3%-3.5% nutrient components. The nutrient components provide the intrinsic motivation for larvae to feed, while the volatile components provide long-distance attraction signals. The two work together to form a dual mechanism of "near-end attraction + far-end guidance", which has a significant attraction effect on various noctuid moth larvae such as cotton bollworm, beet armyworm, beet armyworm, and fall armyworm.

[0021] (2) The feeding attractant for Noctuidae larvae described in this invention uses a combination of five components: fructose, L-proline, inositol, cis-jasmone and n-pentanol, which significantly improves the attraction rate for Noctuidae larvae.

[0022] (3) The feeding attractant for Noctuidae larvae described in this invention uses a weight ratio of 2.0%-3.0% fructose, 0.2%-0.3% L-proline, 0.1%-0.2% inositol, 0.002%-0.003% cis-jasmone and 0.0008%-0.0012% n-pentanol, which is specifically designed for the feeding behavior of Noctuidae larvae and can effectively attract larvae to feed, thereby achieving effective control of the larval stage.

[0023] (4) When the Noctuidae larvae attractant described in this invention is used in combination with pesticides, it has a broad-spectrum attraction effect on Noctuidae larvae, which can reduce the number of times and the amount of pesticides used, avoid the abuse of pesticides, and prevent pests from developing pesticide resistance.

[0024] (5) The Noctuidae larvae feeding attractant described in this invention, when used in combination with pesticides, has no toxic side effects on humans, animals, natural enemies, and the natural environment, and is safe and pollution-free to the environment. Attached Figure Description

[0025] Figure 1 This is a selective device used in insect attraction rate testing.

[0026] Figure 1 In: 1. Treatment group; 2. Control group; 3. Central release zone.

[0027] Figure 2 The results of attracting cotton bollworms with the feeding attractants for Noctuidae larvae in Examples 1-33 are shown. Figure 3 The results of attracting beet armyworm larvae to the feeding attractants of Noctuidae larvae in Examples 1-33 are shown. Figure 4 The results of attracting beet armyworm larvae using the feeding attractants for Noctuidae larvae in Examples 1-33 are shown. Figure 5 The results of attracting fall armyworm using the noctuid larvae attractants of Examples 1-33 are shown.

[0028] Figure 2-5 From left to right, the examples are Example 1 to Example 33. Detailed Implementation

[0029] Example A feeding attractant for Noctuidae larvae, the raw materials for which were prepared are shown in Table 1 by weight percentage.

[0030] A method for preparing a feeding attractant for Noctuidae larvae includes the following steps: Cis-jasmone was pre-dissolved in anhydrous ethanol, and n-pentanol was pre-dissolved in anhydrous ethanol. The two solutions were then mixed to prepare a mother liquor. Fructose, L-proline, and inositol were dissolved in deionized water to prepare an aqueous solution. Adding the mother liquor to an aqueous solution yields a feeding attractant for Noctuidae larvae.

[0031] Table 1

[0032] Note: " / " means not to add.

[0033] Application Example 1 A feeding attractant comprising the Noctuidae larval feeding attractant of Example 28 and 1.8 wt% abamectin emulsifiable concentrate.

[0034] A method for preparing a feeding attractant includes the following steps: adding 15 mL of noctuid moth larvae feeding attractant to 15 L of water and diluting it 1000 times, then adding 50 mL of 1.8 wt% abamectin emulsifiable concentrate and mixing to obtain the feeding attractant.

[0035] Application Example 2 A feeding attractant comprising the Noctuidae larval feeding attractant of Example 28 and 20 wt% chlorantraniliprole suspension.

[0036] A method for preparing a feeding attractant includes the following steps: adding 15 mL of noctuid larvae feeding attractant to 15 L of water and diluting it 1000 times, then adding 5 mL of 20 wt% chlorantraniliprole suspension and mixing to obtain the feeding attractant.

[0037] Application Example 3 A feeding attractant comprising the Noctuidae larval feeding attractant of Example 28 and 10 wt% bromonitrile dibenzodiamide suspension.

[0038] A method for preparing a feeding attractant includes the following steps: adding 15 mL of noctuid larvae feeding attractant to 15 L of water and diluting it 1000 times, then adding 10 mL of 10 wt% bromonitrile dimethyl sulfadiazine suspension and mixing to obtain the feeding attractant.

[0039] Application Comparative Example 1 1.8wt% abamectin emulsifiable concentrate, diluted in 15L of water.

[0040] Application Comparative Example 2 Dilute 20wt% chlorantraniliprole suspension with 15L of water.

[0041] Application Comparative Example 3 Dilute 10wt% bromofenopram suspension with 15L of water.

[0042] Application Comparative Example 4 Clear water.

[0043] Performance testing 1. Insect attraction rate test: In a selective device (see...) Figure 1 In ) as Figure 1 As shown in the figure. One side is treatment group 1, and the other side is control group 2.

[0044] Treatment group: The noctuid moth larvae attractant from Examples 1-33 was diluted 1000 times, and then 20 wt% chlorantraniliprole suspension was added to prepare the attractant. Corn leaves were immersed in the attractant for 3 seconds, then removed, air-dried, and placed on one side of the selective device treatment group. Control group: Corn leaves were treated with 20% chlorantraniliprole suspension, and the treatment process was the same as that of the control group.

[0045] At the start of the experiment, 20 fourth-instar noctuid moth larvae, pre-starved for 6 hours, were placed in the central release area 3 of each device. The device was then placed in a windless location, and the experiment lasted for 6 hours. The insect attraction rate on one side of each treatment group was then recorded. Three sets of experiments were conducted for each formulation, with each set repeated 10 times. The test insects used were cotton bollworm, beet armyworm, cabbage looper, and fall armyworm larvae, with each insect tested independently. The test results are shown in Table 2.

[0046] Table 2

[0047] Note: The data in the table are the mean ± standard deviation of the three replicates. Table 2 shows that the single components (fructose, L-proline, inositol, cis-jasmone, and n-pentanol) all had basic attractant effects (attractant rate ≥50%) on the larvae of four noctuid moths: cotton bollworm, beet armyworm, and fall armyworm. However, the attractant effect was lower than that of the compound formulations of any two components. The attractant rates of the three-component and four-component compound formulations were higher than those of the single-component and two-component compound formulations, but lower than those of the five-component compound formulations in Examples 28-33. Among the four larvae, Example 28 had the highest attractant rate.

[0048] This demonstrates a significant synergistic effect among the five components, and the complete combination of all five components maximizes the attraction effect. The attraction effects of different formulations on the four noctuid moth larvae showed a generally consistent trend, indicating that the attractant of this invention has broad-spectrum attraction activity against these four noctuid moth larvae. The attraction results for cotton bollworms are as follows: Figure 2 The results of attracting the beet armyworm are as follows: Figure 3 The results of attracting beet armyworms are as follows: Figure 4 The results of attracting fall armyworms are as follows: Figure 5 . Figure 2-5 From left to right, the examples are Example 1 to Example 33.

[0049] 2. Field control test: A randomized block design was adopted, with 7 processing groups, each containing 3 cells, and each cell area being 30 m². 2 (6 m × 5 m), and the field management conditions are consistent in each plot.

[0050] The attractants from Examples 1-3 and the agents from Comparative Examples 1-4 were applied. Insect population surveys were conducted 1 day before, 1 day after, 3 days after, and 7 days after spraying. A five-point sampling method was used for each plot. A 50 cm × 50 cm sampling frame was placed at each sampling point. The number of live Noctuidae larvae on all peanut plants within the sampling frame was investigated, and the insect population per square meter was recorded.

[0051] The following formulas are used to calculate the insect population reduction rate and corrected control efficacy: Pest population reduction rate (%) = (Number of pests before spraying - Number of pests after spraying) ÷ Number of pests before spraying × 100%; Control efficacy (%) = (Pest population reduction rate in the treatment group - Pest population reduction rate in the control group) ÷ (1 - Pest population reduction rate in the control group) × 100%. Treatment group: Application of Examples 1-3, application of Comparative Examples 1-3; Control group: Application of Comparative Example 4.

[0052] The test results are shown in Table 3.

[0053] Table 3

[0054] Note: The data in the table are the mean ± standard deviation of three replicates; P <0.05 indicates a significant difference between treatment groups. P >0.05 indicates no significant difference; the control group was not included in the calculation of the prevention and treatment effect.

[0055] Table 3 shows that the control effects of all application examples were higher than those of the comparative examples, proving that the combination of the attractant and the three pesticides can improve the control effect. Application example 2 showed the best control effect, with a control effect of 90.23% after 7 days of spraying, which was 24.9% higher than that of comparative example 2. Application example 3 was the second best, with a control effect of 85.67% after 7 days of spraying, which was 22.65% higher than that of comparative example 3. Application example 1 showed a control effect of 78.35% after 7 days of spraying, which was 16.65% higher than that of comparative example 1. In summary, the combination of the attractant and pesticide of this invention has a significant synergistic effect, and the control effect on pests is more obvious after the combination.

Claims

1. A feeding attractant for Noctuidae larvae, characterized in that, It contains 0.0028%-0.0042% volatile components and 2.3%-3.5% nutrients by weight, with deionized water added to 100%.

2. The noctuid moth larvae attractant according to claim 1, characterized in that, The nutritional components, calculated as a percentage by weight of the attractant for Noctuidae larvae, include 2.0%-3.0% fructose, 0.2%-0.3% L-proline, and 0.1%-0.2% inositol.

3. The noctuid moth larvae attractant according to claim 1, characterized in that, The nutritional components, by weight percentage of the noctuid moth larvae attractant, include 2.5% fructose, 0.25% L-proline, and 0.15% inositol.

4. The noctuid moth larvae attractant according to claim 1, characterized in that, The volatile components, calculated as a percentage by weight of the noctuid moth larvae attractant, include 0.002% to 0.003% cis-jasmone and 0.0008% to 0.0012% n-pentanol; the noctuid moth larvae attractant also includes a solubilizer.

5. A method for preparing a feeding attractant for Noctuidae larvae according to any one of claims 1-4, characterized in that, Includes the following steps: The volatile components are dissolved in a co-solvent to prepare a mother liquor; The nutrient components were dissolved in deionized water to prepare an aqueous solution; Adding the mother liquor to an aqueous solution yields a feeding attractant for Noctuidae larvae.

6. An appetite stimulant, characterized in that, Includes the noctuid moth larvae attractant and agent as described in any one of claims 1-4.

7. The appetite-stimulating agent according to claim 6, characterized in that, The volume-to-mass ratio of the food attractant to the drug is 15 mL: (0.9-1.1) g.

8. A method for preparing the appetite stimulant according to any one of claims 6-7, characterized in that, Includes the following steps: The attractant for noctuid moth larvae is diluted and mixed with the pesticide to obtain the attractant.

9. The method for preparing the appetite stimulant according to claim 8, characterized in that, The dilution factor is 900-1200 times.

10. The application of an appetite-stimulating agent according to any one of claims 6-7, characterized in that, It is used in the control of cotton bollworm, beet armyworm, and fall armyworm.

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