A method for extracting microbial nucleic acids from raw milk

By combining microwave heating and TE buffer with low-speed centrifugation, the safety, efficiency, and cost issues of microbial nucleic acid extraction from raw milk have been solved, achieving rapid, safe, and low-cost nucleic acid extraction, which is suitable for raw milk quality inspection and control.

CN122256331APending Publication Date: 2026-06-23INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
Filing Date
2026-02-04
Publication Date
2026-06-23

AI Technical Summary

Technical Problem

Existing technologies for extracting microbial nucleic acids from raw milk suffer from problems such as high safety risks, cumbersome operation, long time consumption, high cost, difficulty in automation, and low nucleic acid purity.

Method used

A method combining microwave heating with high-density TE buffer and low-speed centrifugation is used to break down microbial cell walls, and then nucleic acid extraction is performed using a DNA adsorption column, avoiding the use of harmful reagents and simplifying the operation process.

Benefits of technology

It enables rapid, safe, and low-cost nucleic acid extraction, improves the purity and integrity of nucleic acids, and is suitable for downstream detection with high requirements such as real-time quantitative PCR, while reducing operational complexity and time costs.

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Abstract

The present application relates to the technical field of microorganism detection, and particularly relates to a method for extracting microbial nucleic acid in raw milk. The present application can efficiently and quickly break the microbial cell wall in raw milk by combining microwave heating, high-density TE buffer and low-speed centrifugal treatment, realizes the rapid extraction of microbial nucleic acid in raw milk, and reduces the time and cost of nucleic acid extraction. The present application provides a brand-new technical path for realizing the rapid extraction of microbial nucleic acid in raw milk, and has a broad application prospect in raw milk quality inspection or quality control.
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