A triple lyophilized fluorescent PCR rapid detection kit for canine distemper virus, canine coronavirus, and canine parvovirus.

Abstract

This invention discloses a triple lyophilized fluorescent PCR rapid detection kit for canine distemper virus, canine coronavirus, and canine parvovirus, belonging to the field of rapid detection kit technology. It includes a housing, protective components, and a sample collection mechanism. The back of the housing is symmetrically fitted with hinges via bolts. This triple lyophilized fluorescent PCR rapid detection kit for canine distemper virus, canine coronavirus, and canine parvovirus, through its primer, fluorescent probe, and reagent carrier components and sample collection mechanism, enables the rapid screening of three highly pathogenic canine viruses (canine distemper virus CDV, canine coronavirus CCV, and canine parvovirus CPV) using a lyophilization process based on quantitative real-time PCR technology.

Description

Technical Field

[0001] This invention relates to the field of rapid detection kit technology, specifically a triple lyophilized fluorescent PCR rapid detection kit for canine distemper virus, canine coronavirus, and canine parvovirus. Background Technology

[0002] The lyophilized fluorescent PCR rapid test kit is a molecular diagnostic tool that combines lyophilization technology and quantitative fluorescent PCR technology. It is used to rapidly detect specific nucleic acid sequences. However, when it comes to detecting common deadly viruses in dogs, a specialized rapid test kit is required.

[0003] First, the existing rapid test kits have a relatively simple detection mode, and can generally only detect one type of virus. As a result, users often need to use multiple different rapid test kits for multiple tests when screening for viruses. This is not only cumbersome to operate, but also causes unnecessary waste of samples and greatly reduces the detection efficiency. Secondly, the existing rapid test kits often use screw-on, manually pry-open buckle, or plug-in outer cap designs for sealing the reagent tubes and kits. This requires users to repeatedly screw or forcefully pry open the buckles, which is not only time-consuming and laborious, but also prone to causing the tubes to tip over and reagents to spill due to improper operation. Furthermore, non-professionals (such as pet owners) are prone to breaking buckles or not tightening the threads properly, which cannot guarantee the sealing effect. In addition, the sealing protection design is not perfect, which can easily lead to sample or reagent contamination and reduce the accuracy of the test, thus having certain defects in use. Summary of the Invention

[0004] The purpose of this invention is to provide a triple lyophilized fluorescent PCR rapid detection kit for canine distemper virus, canine coronavirus, and canine parvovirus, in order to solve the problems mentioned in the background art.

[0005] To achieve the above objectives, the present invention provides the following technical solution: a triple lyophilized fluorescent PCR rapid detection kit for canine distemper virus, canine coronavirus, and canine parvovirus, comprising a storage box, a protective component, and a sample sampling mechanism. The back of the storage box is symmetrically fitted with hinges by bolts, and a sealing component is fitted on the other side of the hinges by bolts. A connecting bracket is fixedly installed on the front of the storage box. The protective component is fixedly installed inside the storage box, and a primer fluorescent probe reagent carrier component and a sample sampling mechanism are engaged inside the protective component.

[0006] Furthermore, the sealing assembly includes a sealing cover, and an L-shaped locking plate is fixedly installed at the end of the sealing cover. A bearing groove is provided on the outer side of the L-shaped locking plate, and a first elastic connecting mechanism is installed inside the bearing groove. At the same time, a first arc-shaped button is fixedly connected to the end of the first elastic connecting mechanism.

[0007] Furthermore, the end of the first elastic connecting mechanism is fixedly connected to the end of the first arc-shaped button, and the other end of the first elastic connecting mechanism is fixedly connected to the inner surface of the L-shaped locking plate. The first arc-shaped button forms an elastic structure with the L-shaped locking plate through the first elastic connecting mechanism, and the other end of the first arc-shaped button is engaged with the inside of the connecting bracket.

[0008] Furthermore, the protective component includes a carrier tube sponge pad, and a storage tube storage sponge pad is fixedly connected to the end of the carrier tube sponge pad. A limiting sponge pad is attached to the top of the carrier tube sponge pad, and the top of the limiting sponge pad is fixedly connected to the bottom of the sealing cover. At the same time, storage slots are provided at equal intervals on the bottom left side of the limiting sponge pad.

[0009] Furthermore, the side of the carrier tube sponge pad is attached and fixedly connected to the side of the storage tube receiving sponge pad, and the carrier tube sponge pad is used to carry and store the primer fluorescent probe reagent carrier assembly, and the storage tube receiving sponge pad is used to carry and store the sample sampling mechanism. Moreover, the top of the storage tube receiving sponge pad is symmetrically provided with grooves to facilitate the retrieval of the sample sampling mechanism.

[0010] Furthermore, the primer fluorescent probe reagent carrier assembly includes a tube, and the upper end of the tube is symmetrically provided with locking grooves, and a sealing mechanism is engaged and installed at the top of the tube.

[0011] Furthermore, the sealing mechanism includes a main cover, and a fixed connecting end is fixedly installed at the bottom of the main cover. A flexible sealing plug is fixedly connected to the bottom of the fixed connecting end. A second elastic connecting mechanism is symmetrically embedded and movably installed on both sides of the fixed connecting end, and a second arc-shaped button is fixedly connected to the end of the second elastic connecting mechanism.

[0012] Furthermore, the external dimensions of the main cover match the internal dimensions of the storage slot, and the main cover forms a locking structure with the limiting sponge pad through the storage slot.

[0013] Furthermore, the end of the second elastic connecting mechanism is fixedly connected to the end of the second arc-shaped button, and the other end of the second elastic connecting mechanism is fixedly connected to the inner surface of the fixed connecting end. The second arc-shaped button forms an elastic structure with the fixed connecting end through the second elastic connecting mechanism, and the second arc-shaped button is engaged with the tube body through a locking groove.

[0014] Furthermore, the sample collection mechanism includes a saline storage tube, and a connecting cap is fitted to the top of the saline storage tube. An annular threaded sealing block is fixedly installed at the bottom of the connecting cap. A rod is fixedly connected through the inside of the annular threaded sealing block, and a cotton sampling head is fixedly connected to the bottom of the rod. The outer surface of the annular threaded sealing block is threadedly connected to the inner surface of the saline storage tube.

[0015] This invention provides a triple lyophilized fluorescent PCR rapid detection kit for canine distemper virus, canine coronavirus, and canine parvovirus, which has the following beneficial effects: 1. This invention, through its primer-fluorescent probe reagent carrier component and sample collection mechanism, enables the rapid screening of three highly pathogenic canine viruses (canine distemper virus CDV, canine coronavirus CCV, and canine parvovirus CPV) using a lyophilization process based on quantitative real-time PCR technology. Furthermore, because the primer-fluorescent probe reagent carrier component simultaneously carries corresponding primers and fluorescent probes for all three viruses, users can directly detect the corresponding virus type in a single test, improving both convenience and efficiency (eliminating the need for repeated testing). Moreover, the lyophilization process allows for storage and transportation at room temperature, significantly reducing storage and transportation costs and facilitating the widespread application of rapid diagnostic kits.

[0016] 2. This invention features an automatic unlocking structure composed of a press-type arc-shaped button and an elastic connection mechanism on both the sealing component and the primer / fluorescent probe reagent carrier component. This allows users to quickly unlock the device by pressing with their fingers, and to lock it by pressing in the opposite direction. This greatly simplifies the operation process and lowers the operational threshold. Furthermore, the reagent carrier component and the primer / fluorescent probe reagent carrier component are equipped with flexible sealing plugs. When the sealing mechanism locks, the flexible sealing plugs cooperate with the tube body to achieve a seal, preventing leakage or evaporation of sample diluents and reaction solvents. At the same time, it prevents external dust and bacteria from entering the tube body and contaminating the reagents, ensuring a pure reaction environment for the primers and fluorescent probes with the sample. It also effectively protects reagents, detection instruments, and other accessories from contamination before detection. Attached Figure Description

[0017] Figure 1 This is a frontal view of the three-dimensional structure of a triple lyophilized fluorescent PCR rapid detection kit for canine distemper virus, canine coronavirus, and canine parvovirus according to the present invention. Figure 2This is a rear-view closed three-dimensional structural diagram of a triple lyophilized fluorescent PCR rapid detection kit for canine distemper virus, canine coronavirus, and canine parvovirus according to the present invention. Figure 3 This is a frontal view of the three-dimensional structure of a triple lyophilized fluorescent PCR rapid detection kit for canine distemper virus, canine coronavirus, and canine parvovirus according to the present invention. Figure 4 This invention relates to a triple lyophilized fluorescent PCR rapid detection kit for canine distemper virus, canine coronavirus, and canine parvovirus. Figure 3 Enlarged schematic diagram of the structure at point A in the middle; Figure 5 This is a schematic diagram of the tube-main cap disassembled three-dimensional structure of a triple freeze-dried fluorescent PCR rapid detection kit for canine distemper virus, canine coronavirus and canine parvovirus according to the present invention. Figure 6 This is a three-dimensional structural diagram of the sample collection mechanism of a triple lyophilized fluorescent PCR rapid detection kit for canine distemper virus, canine coronavirus, and canine parvovirus according to the present invention.

[0018] In the diagram: 1. Storage box; 2. Hinge; 3. Sealing assembly; 31. Sealing cap; 32. L-shaped locking plate; 33. Support groove; 34. First elastic connection mechanism; 35. First arc-shaped button; 4. Connecting bracket; 5. Protective assembly; 51. Support tube sponge pad; 52. Storage tube storage sponge pad; 53. Limiting sponge pad; 54. Storage slot; 6. Primer fluorescent probe reagent support assembly; 61. Tube body; 62. Locking groove; 63. Sealing mechanism; 631. Main cap; 632. Fixed connection end; 633. Flexible sealing plug; 634. Second elastic connection mechanism; 635. Second arc-shaped button; 7. Sample sampling mechanism; 71. Physiological saline storage tube; 72. Connecting cap; 73. Annular threaded sealing block; 74. Rod body; 75. Cotton sampling head. Detailed Implementation

[0019] The embodiments of the present invention will be described in further detail below with reference to the accompanying drawings and examples. The following examples are for illustrative purposes only and should not be construed as limiting the scope of the invention.

[0020] like Figures 1-3As shown, a triple lyophilized fluorescent PCR rapid detection kit for canine distemper virus, canine coronavirus, and canine parvovirus includes a storage box 1, a protective component 5, and a sample collection mechanism 7. Hinges 2 are symmetrically mounted on the back of the storage box 1 via bolts, and a sealing component 3 is mounted on the other side of the hinges 2 via bolts. The sealing component 3 includes a sealing cap 31, and an L-shaped locking plate 32 is fixedly mounted at the end of the sealing cap 31. A support groove 33 is formed on the outer side of the L-shaped locking plate 32, and a first elastic connecting mechanism 34 is installed inside the support groove 33. A first arc-shaped button 35 is fixedly connected to the end of the first elastic connecting mechanism 34, and the end of the first elastic connecting mechanism 34 is fixed to the end of the first arc-shaped button 35. The first elastic connecting mechanism 34 is connected to the inner surface of the L-shaped locking plate 32, and the first arc-shaped button 35 forms an elastic structure with the L-shaped locking plate 32 through the first elastic connecting mechanism 34. The other end of the first arc-shaped button 35 is engaged with the interior of the connecting bracket 4. Through the elastic structure of the first arc-shaped button 35 and the L-shaped locking plate 32, when the user moves the L-shaped locking plate 32 towards the interior of the connecting bracket 4, the first arc-shaped button 35 can automatically engage with the interior of the connecting bracket 4 through its rebound force to complete the self-locking operation. The connecting bracket 4 is fixedly installed on the front of the storage box 1, and the protective component 5 is fixedly installed inside the storage box 1. The protective component 5 includes... The device includes a carrier tube sponge pad 51, with a storage tube receiving sponge pad 52 fixedly connected to the end of the carrier tube sponge pad 51. The sides of the carrier tube sponge pad 51 and the storage tube receiving sponge pad 52 are fixedly connected. The carrier tube sponge pad 51 is used to carry and store the primer fluorescent probe reagent carrier assembly 6, and the storage tube receiving sponge pad 52 is used to carry and store the sample sampling mechanism 7. The top of the storage tube receiving sponge pad 52 has symmetrically opened grooves for easy retrieval of the sample sampling mechanism 7. A limiting sponge pad 53 is attached to the top of the carrier tube sponge pad 51, and the top of the limiting sponge pad 53 is fixedly connected to the bottom of the sealing cap 31. At the same time, storage clips are evenly spaced on the bottom left side of the limiting sponge pad 53. The groove 54 and the protective component 5 are internally fitted with a primer fluorescent probe reagent carrier component 6 and a sample sampling mechanism 7. The sample sampling mechanism 7 includes a saline storage tube 71, and a connecting cap 72 is attached to the top of the saline storage tube 71. An annular threaded sealing block 73 is fixedly installed at the bottom of the connecting cap 72. A rod 74 is fixedly connected through the annular threaded sealing block 73, and a cotton sampling head 75 is fixedly connected to the bottom of the rod 74. The outer surface of the annular threaded sealing block 73 is threadedly connected to the inner surface of the saline storage tube 71. The setting of the annular threaded sealing block 73 ensures that the cap 72 will not detach during the process of shaking and diluting the sample.

[0021] like Figures 3-6As shown, hinges 2 are symmetrically installed on the back of the storage box 1 via bolts, and a sealing component 3 is installed on the other side of the hinges 2 via bolts. A connecting bracket 4 is fixedly installed on the front of the storage box 1. A protective component 5 is fixedly installed inside the storage box 1, and a primer fluorescent probe reagent carrier component 6 and a sample sampling mechanism 7 are locked inside the protective component 5. The primer fluorescent probe reagent carrier component 6 includes a tube 61, and locking grooves 62 are symmetrically opened at the upper end of the tube 61. The tube 61 contains the upstream primer (CPV-F) for canine parvovirus: 5'-TGG GGA AAG AGT TGGTAT TTT GA-3' (23 bp, Tm=59.5℃), the downstream primer (CPV-R): 5'-CCA TCC ACA TCC TCC TGCTTA-3' (21 bp, Tm=59.2℃), and the fluorescent probe (CPV-P): 5'-FAM-TCA GCA TCA CCA CCA TCA GCCTTG T-BHQ1-3' (25 bp, Tm=68.0℃); upstream primer for canine distemper virus (CDV-F): 5'-GGA TGT GGA GATGGA TGT GA-3' (20 bp, Tm=58.8℃), downstream primer (CDV-R): 5'-GCT GCT TGA AGT GGT TGT GA-3' (20 bp, Tm=58.5℃), fluorescent probe (CDV-P): 5'-HEX-TCA CCA GCA GGA CCA GCA TCA TCA T-BHQ1-3' (24 bp, Tm=67.2℃); and upstream primer for canine coronavirus (CCV-F): 5'-GCT GGA GGA TGAGAA GGT GA-3' (20 bp, Tm=59.0℃), downstream primer (CCV-R): 5'-TCC TTG CTT CTT GGT GGT GT-3' (20 bp, Tm=58.7℃), fluorescent probe (CCV-P): 5'-ROX-TGA ACA CCA CCA CCA TCA CCA TCA T-BHQ2-3' (24 bp, Tm=68.5℃). The lyophilization formulation also includes trehalose and mannitol as protective agents to reduce the impact of the lyophilization process on enzyme activity. The lyophilization process consists of a pre-freezing stage (temperature maintained between -40~-50℃ for 2~3 hours) and sublimation drying (vacuum: 10~30Pa; heating rate controlled at 0).(5~1℃ / h) and analytical drying (temperature needs to be maintained between 25~30℃ and kept at that temperature for 4~6 hours). The qualified criteria after freeze-drying are: the reagent is a loose, porous white or pale yellow powder, without clumping, collapse, or sticking to the wall; at the same time, there are no obvious liquid droplets remaining at the bottom of the tube; and reconstitution can be carried out at 30℃. The solution dissolves completely within seconds, leaving a clear, precipitate-free solution with a residual water content of ≤3%, while simultaneously reducing production costs. A sealing mechanism 63 is fitted onto the top of the tube body 61. This sealing mechanism 63 includes a main cover body 631, with a fixed connecting end 632 fixedly installed at the bottom. The external dimensions of the main cover body 631 match the internal dimensions of the receiving slot 54. The main cover body 631, through the receiving slot 54, forms a locking structure with the limiting sponge pad 53. This locking structure of the main cover body 631 and the limiting sponge pad 53 allows the top of the sealing mechanism 63 to be easily retracted into the limiting sponge pad 53 for limiting protection when the sealing assembly 3 is closed. A flexible sealing plug 633 is fixedly connected to the bottom of the fixed connecting end 632, and both sides of the fixed connecting end 632... A second elastic connecting mechanism 634 is symmetrically and movably installed. One end of the second elastic connecting mechanism 634 is fixedly connected to the end of the second arc-shaped button 635, and the other end of the second elastic connecting mechanism 634 is fixedly connected to the inner surface of the fixed connecting end 632. The second arc-shaped button 635 forms an elastic structure with the fixed connecting end 632 via the second elastic connecting mechanism 634. The second arc-shaped button 635 is engaged with the tube body 61 via a locking groove 62. Due to the elastic structure of the second arc-shaped button 635 and the fixed connecting end 632, when the user presses down on the sealing mechanism 63, the second arc-shaped button 635 automatically springs back and engages with the locking groove 62 to complete the locking operation. Simultaneously, the second arc-shaped button 635 is fixedly connected to the end of the second elastic connecting mechanism 634.

[0022] In summary, combining Figures 1-6As shown, the working principle of this triple lyophilized fluorescent PCR rapid detection kit for canine distemper virus, canine coronavirus, and canine parvovirus is as follows: First, the operator removes the protective film from the outside of the kit. Then, the operator presses the first arc-shaped button 35 inward, causing the first arc-shaped button 35 to squeeze the first elastic connecting mechanism 34 towards the inside of the support groove 33. When the outer end of the first arc-shaped button 35 separates from the inside of the connecting bracket 4, the sealing cover 31 is opened by rotating the hinge 2. Then, the user takes out the sample sampling mechanism 7 from inside the storage tube receiving sponge pad 52. Next, the user grasps the connecting cover 72 and rotates the annular threaded sealing block 73, causing the connecting cover 72 to separate from the saline storage tube 71. Then, the cotton sampling head 75 is taken out by the rod 74 and inserted into the saline storage tube. During the sampling process, once the surface of the cotton sampling head 75 is covered with sample, it is moved back into the saline storage tube 71 and sealed and fixed by threads. Then, the user shakes the saline storage tube 71 to dilute the sample through the saline inside the saline storage tube 71. Next, the user takes out a primer fluorescent probe reagent carrier component 6 from inside the carrier tube sponge pad 51. Then, the user presses the main cover 631 with the index finger and presses the second arc-shaped buttons 635 on both sides with the thumb and middle finger, so that the second arc-shaped buttons 635 squeeze the second elastic connection mechanism 634 to move inward toward the fixed connection end 632, thereby achieving automatic unlocking. Then, the user grabs the main cover 631 and pulls it upward to remove the sealing mechanism 63 from the top of the tube 61. Next, the user adds the diluted sample supernatant into the tube 61, and then presses the sealing mechanism 63 back into the tube 61. At this time, the self-locking work is completed by the cooperation of the sealing mechanism 63, and the sealing work is completed by the cooperation of the flexible sealing plug 633. Then, the user's index finger, thumb and middle finger press on the anti-slip texture on the top of the main cap 631 and the outer surface of the tube 61, respectively. Then, shake the primer fluorescent probe reagent carrier component 6 for three minutes to fully mix the sample with the solvent in the tube 61. At this time, if the sample contains one or more of canine distemper virus, canine coronavirus, and canine parvovirus, the corresponding primers and fluorescent probes in the tube 61 will react. After standing for five minutes, the primer fluorescent probe reagent carrier component 6, together with the detection solution inside, is sealed and placed into the detector of the fluorescence PCR instrument for automatic detection processing. Finally, if the instrument shows a typical amplification curve in a certain fluorescence channel, the corresponding virus is positive; if there is no amplification curve, it indicates a negative result.

[0023] The embodiments of the present invention are given for illustrative and descriptive purposes only, and are not intended to be exhaustive or to limit the invention to the forms disclosed. Many modifications and variations will be apparent to those skilled in the art. The embodiments were chosen and described in order to better illustrate the principles and practical application of the invention, and to enable those skilled in the art to understand the invention and to design various embodiments with various modifications suitable for a particular purpose.

Claims

1. A triple lyophilized fluorescent PCR rapid detection kit for canine distemper virus, canine coronavirus, and canine parvovirus, comprising a storage box (1), a protective component (5), and a sample collection mechanism (7), characterized in that, The back of the storage box (1) is symmetrically fitted with hinges (2) by bolts, and the other side of the hinges (2) is fitted with a sealing assembly (3) by bolts. The front of the storage box (1) is fixedly fitted with a connecting bracket (4). The protective assembly (5) is fixedly installed inside the storage box (1), and the primer fluorescent probe reagent carrier assembly (6) and the sample sampling mechanism (7) are locked inside the protective assembly (5).

2. The canine distemper virus, canine coronavirus, and canine parvovirus triple lyophilized fluorescent PCR rapid detection kit according to claim 1, characterized in that, The sealing assembly (3) includes a sealing cover (31), and an L-shaped locking plate (32) is fixedly installed at the end of the sealing cover (31). A bearing groove (33) is provided on the outer side of the L-shaped locking plate (32), and a first elastic connecting mechanism (34) is installed inside the bearing groove (33). At the same time, a first arc-shaped button (35) is fixedly connected to the end of the first elastic connecting mechanism (34).

3. The canine distemper virus, canine coronavirus, and canine parvovirus triple lyophilized fluorescent PCR rapid detection kit according to claim 2, characterized in that, The end of the first elastic connecting mechanism (34) is fixedly connected to the end of the first arc-shaped button (35), and the other end of the first elastic connecting mechanism (34) is fixedly connected to the inner surface of the L-shaped locking plate (32). The first arc-shaped button (35) forms an elastic structure with the L-shaped locking plate (32) through the first elastic connecting mechanism (34), and the other end of the first arc-shaped button (35) is engaged with the inside of the connecting bracket (4).

4. The canine distemper virus, canine coronavirus, and canine parvovirus triple lyophilized fluorescent PCR rapid detection kit according to claim 2, characterized in that, The protective component (5) includes a carrier tube sponge pad (51), and a storage tube storage sponge pad (52) is attached and fixedly connected to the end of the carrier tube sponge pad (51). A limiting sponge pad (53) is attached to the top of the carrier tube sponge pad (51), and the top of the limiting sponge pad (53) is fixedly connected to the bottom of the sealing cover (31). Meanwhile, storage slots (54) are provided at equal intervals on the left bottom of the limiting sponge pad (53).

5. The canine distemper virus, canine coronavirus, and canine parvovirus triple lyophilized fluorescent PCR rapid detection kit according to claim 4, characterized in that, The side of the carrier tube sponge pad (51) is attached and fixedly connected to the side of the storage tube receiving sponge pad (52). The carrier tube sponge pad (51) is used to carry the primer fluorescent probe reagent carrier assembly (6), and the storage tube receiving sponge pad (52) is used to carry the sample sampling mechanism (7). The top of the storage tube receiving sponge pad (52) is symmetrically provided with grooves to facilitate the retrieval of the sample sampling mechanism (7).

6. The canine distemper virus, canine coronavirus, and canine parvovirus triple lyophilized fluorescent PCR rapid detection kit according to claim 4, characterized in that, The primer fluorescent probe reagent carrier assembly (6) includes a tube (61), and the upper end of the tube (61) is symmetrically provided with locking grooves (62), and the top of the tube (61) is fitted with a sealing mechanism (63).

7. The canine distemper virus, canine coronavirus, and canine parvovirus triple lyophilized fluorescent PCR rapid detection kit according to claim 6, characterized in that, The sealing mechanism (63) includes a main cover (631), and a fixed connection end (632) is fixedly installed at the bottom of the main cover (631). A flexible sealing plug (633) is fixedly connected to the bottom of the fixed connection end (632). A second elastic connection mechanism (634) is symmetrically embedded and movably installed on both sides of the fixed connection end (632). At the same time, a second arc-shaped button (635) is fixedly connected to the end of the second elastic connection mechanism (634).

8. The canine distemper virus, canine coronavirus, and canine parvovirus triple lyophilized fluorescent PCR rapid detection kit according to claim 7, characterized in that, The external dimensions of the main cover (631) match the internal dimensions of the storage slot (54), and the main cover (631) and the limiting sponge pad (53) form a locking structure through the storage slot (54).

9. The canine distemper virus, canine coronavirus, and canine parvovirus triple lyophilized fluorescent PCR rapid detection kit according to claim 7, characterized in that, The end of the second elastic connecting mechanism (634) is fixedly connected to the end of the second arc-shaped button (635), and the other end of the second elastic connecting mechanism (634) is fixedly connected to the inner surface of the fixed connecting end (632). The second arc-shaped button (635) forms an elastic structure with the fixed connecting end (632) through the second elastic connecting mechanism (634), and the second arc-shaped button (635) is engaged with the tube body (61) through the locking groove (62).

10. The canine distemper virus, canine coronavirus, and canine parvovirus triple lyophilized fluorescent PCR rapid detection kit according to claim 1, characterized in that, The sample collection mechanism (7) includes a saline storage tube (71), and a connecting cap (72) is fitted to the top of the saline storage tube (71). An annular threaded sealing block (73) is fixedly installed at the bottom of the connecting cap (72). A rod (74) is fixedly connected through the annular threaded sealing block (73), and a cotton sampling head (75) is fixedly connected to the bottom of the rod (74). The outer surface of the annular threaded sealing block (73) is threadedly connected to the inner surface of the saline storage tube (71).

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