NK cell induction expansion culture additive, culture medium and application thereof
By using a culture additive composed of tanshinone I and berberine, which binds to IL-2 and IL-21, the memory phenotype reprogramming of NK cells is promoted, solving the problems of low NK cell transformation efficiency and high cost in existing technologies, and realizing efficient and safe memory NK cell culture.
CN122278765APending Publication Date: 2026-06-26SUZHOU EXCELL BIOLOGICAL TECH CO LTD +1
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Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- SUZHOU EXCELL BIOLOGICAL TECH CO LTD
- Filing Date
- 2026-05-28
- Publication Date
- 2026-06-26
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Figure CN122278765A_ABST
Abstract
This invention relates to an additive for NK cell induction and expansion culture, a culture medium, and their applications. The additive comprises tanshinone I and berberine. Specifically, PBMCs are isolated from human peripheral blood. On the third day of NK cell activation culture, tanshinone I (final concentration 0.1-10 µM) and berberine (final concentration 10-100 µM) are added to the culture system. Both tanshinone I and berberine are natural products with wide availability. Their combined use can significantly increase the proportion of memory NK cells, while exhibiting high viability, high purity, high expansion rate, and high cytotoxic activity. Flow cytometry analysis showed a significant increase in the positive expression rates of some NK cell markers, including CD62L, CD127, and Eomes.
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