Core SNP molecular marker combinatorial for seed setting rate of Leymus chinensis developed based on KASP technology and its application

By developing a core SNP molecular marker combination for seed setting rate of Leymus chinensis based on KASP technology, the problem of low seed setting rate of Leymus chinensis was solved, enabling rapid and accurate identification and efficient breeding of Leymus chinensis germplasm resources, and improving seed yield and germplasm resource management efficiency.

CN122279094APending Publication Date: 2026-06-26CHINA AGRI UNIV
View PDF 0 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
CHINA AGRI UNIV
Filing Date
2026-05-26
Publication Date
2026-06-26

AI Technical Summary

Technical Problem

Low seed setting rate of Leymus chinensis is a bottleneck restricting the development of its industry. Existing technologies lack effective molecular marker methods to improve seed yield and accelerate molecular breeding progress.

Method used

We developed a core SNP molecular marker combinatorial system for seed setting rate of Leymus chinensis based on KASP technology, which includes 120 SNP molecular markers and corresponding KASP primer combinations. This combinatorial system was used for germplasm identification, breeding and genetic analysis of Leymus chinensis. DNA was extracted using the CTAB method and genotyped on an ABI 7500 real-time quantitative PCR instrument.

Benefits of technology

It enables rapid and accurate identification and high-throughput SNP typing of Leymus chinensis germplasm resources, increases seed yield, simplifies the identification and breeding process of germplasm resources, and protects the interests of breeders and farmers.

✦ Generated by Eureka AI based on patent content.

Smart Images

  • Figure FT_1
    Figure FT_1
  • Figure FT_2
    Figure FT_2
  • Figure FT_3
    Figure FT_3
Patent Text Reader

Abstract

This invention belongs to the fields of molecular biology and plant molecular breeding technology, and discloses a core SNP molecular marker combination for seed setting rate of Leymus chinensis developed based on KASP technology and its application. The core SNP molecular marker combination for seed setting rate of Leymus chinensis contains 120 pairs of KASP markers, obtained through deep genome sequencing of 300 Leymus chinensis germplasm resources. Based on genome-wide association analysis of the seed setting rate trait, 120 pairs of KASP molecular markers were obtained. Simultaneously, cluster analysis and population structure analysis were performed on the 300 Leymus chinensis accessions to obtain 50 superior KASP genotypes of high-seed-setting-rate Leymus chinensis germplasm. The core molecular marker combination for Leymus chinensis developed by this invention based on KASP technology can rapidly, accurately, and effectively identify and evaluate Leymus chinensis resources and detect purity. It enables high-throughput SNP genotyping detection of Leymus chinensis materials, thereby establishing a simpler, more efficient, and lower-cost molecular marker-assisted breeding method for Leymus chinensis variety authenticity, seed purity, and seed setting rate.
Need to check novelty before this filing date? Find Prior Art