A High-Efficiency Cultivation Method for Cistanche deserticola Inoculated with Trichosanthes kirilowii

Abstract

This invention provides a highly efficient cultivation method for *Cistanche deserticola* inoculated with *Trichosanthes kirilowii*. By employing a composite seed treatment method combining low-temperature precooling, warm water soaking, and variable-temperature stratification, the dormancy of *Cistanche deserticola* seeds can be synergistically broken, significantly improving seed germination potential and uniformity. By designing deep inoculation pits inclined towards the host root system, combined with a double-layer three-dimensional sowing pattern (bottom and middle layers) and backfilling with a nutrient substrate containing vermiculite, the *Trichosanthes kirilowii* roots are actively induced to grow directionally towards the seed-dense area, greatly expanding the parasitic interface and steadily increasing the inoculation success rate. Targeted supply of mineral-derived potassium humate and high-phosphorus-potassium fertilizer during the germination and swelling stages of *Cistanche deserticola* not only conserves precious water resources in arid areas but also significantly promotes the accumulation of dry matter in *Cistanche deserticola*, resulting in a substantial increase in the yield per acre of fresh *Cistanche deserticola*. Simultaneously, post-harvest host care measures ensure continued production in the following year, ultimately achieving an organic unity between ecological restoration and high-value medicinal material production.

Description

Technical Field

[0001] This invention relates to the field of plant cultivation technology, and in particular to a highly efficient cultivation method for Cistanche deserticola inoculated with Trichosanthes kirilowii. Background Technology

[0002] Cistanche deserticola, a precious perennial parasitic herb, is known as "desert ginseng" due to its extremely high medicinal and economic value. Traditionally, Cistanche deserticola has relied mainly on Haloxylon ammodendron as a host for cultivation. However, Haloxylon ammodendron grows slowly, has demanding requirements for soil and climate conditions, and is mainly distributed in specific habitats in the arid northwest of my country. This has greatly limited the effective expansion of Cistanche deserticola cultivation areas and the scale of the industry. In recent years, with the increasing urgency of desertification control and ecological restoration, finding a rapidly growing, more adaptable, and ecologically and economically valuable alternative host has become a research hotspot in this field. Trichoderma tetragonum, a semi-evergreen shrub of the Chenopodiaceae family introduced from North America, has been successfully introduced and widely used in windbreak and sand fixation and vegetation restoration projects in the arid and semi-arid northwest of my country due to its strong cold resistance, drought resistance, salt and alkali tolerance, and tolerance to poor soil conditions, as well as its well-developed root system and large biomass.

[0003] Although preliminary explorations have been made into using *Trichoderma tetrawingense* as a host for *Cistanche deserticola*, existing methods still face numerous technical bottlenecks, resulting in low inoculation success rates, unstable yields, and difficulties in large-scale promotion. For example, patent application CN119836988A discloses a method for inoculating *Trichoderma tetrawingense* into *Cistanche deserticola*, which involves soaking seeds in the plant growth regulator gibberellin to break dormancy. During inoculation, only small pits 5-10 cm deep are dug, the seeds are mixed with humus and scattered in, and then covered with plastic film. This method has revealed the following problems and drawbacks in practice: First, its seed treatment method is simplistic, relying solely on gibberellin soaking, failing to adequately and synergistically break the physiological dormancy of *Cistanche deserticola* seeds, leading to uneven and weak germination. Second, the inoculation pit design is too simple and shallow, and it does not consider directional induction of host root growth, resulting in a low probability of seed-to-*Trichoderma tetrawingense* root contact and a small parasitic interface. This is the fundamental reason why the reported inoculation success rate is only above 80%. Secondly, its cultivation and management measures are extensive, lacking targeted and refined design for post-inoculation water and fertilizer management. In particular, it lacks technical means to accurately deliver water and nutrients to the parasitic interface, resulting in a waste of water and fertilizer resources and failing to provide the optimal microenvironment for establishing the parasitic relationship and the later expansion of Cistanche deserticola. In addition, the above methods rarely mention the nurturing and management of the host plant after harvest, which is not conducive to achieving stable yields of Cistanche deserticola year after year.

[0004] Therefore, there is an urgent need in this field for a highly efficient cultivation method that can significantly improve the uniformity of seed germination, parasitism success rate, and yield per unit area of ​​*Cistanche deserticola* inoculated with *Trichosanthes kirilowii*, and that is standardized in operation, has high water and fertilizer utilization efficiency, and is easy to scale up, so as to solve the above-mentioned bottleneck problems in the existing technology and truly achieve a win-win situation for ecological restoration and economic benefits in desertified areas. Summary of the Invention

[0005] The purpose of this invention is to provide a highly efficient cultivation method for Cistanche deserticola inoculated with Trichosanthes kirilowii, in order to solve the problems existing in the prior art.

[0006] To achieve the above objectives, the present invention provides the following solution: This invention provides a highly efficient cultivation method for Cistanche deserticola inoculated with Trichosanthes kirilowii, comprising the following steps: S1: Site selection and host cultivation: Select sandy loam or light loam soil plots with a soil salinity of 0.2%-0.6% and a groundwater level below 1.5m; plant four-winged cattails using a wide-narrow row pattern, with a wide row spacing of 3-4m, a narrow row spacing of 1.0-1.5m, and a plant spacing of 1.0-1.5m. After planting, water thoroughly to settle the roots and carry out nurturing and management for no less than one year, during which winter irrigation is required; S2: Compound treatment of Cistanche deserticola seeds: Cistanche deserticola seeds are first pre-cooled at 4℃ for 7-10 days, then soaked in 40-45℃ warm water for 24 hours, and then mixed with wet sand, and successively stratified at 20-25℃ for 10-15 days and at 4℃ for 15-20 days. S3: Directional three-dimensional inoculation: In the spring or autumn of the year following the host plantation, dig an inoculation pit at a 30° angle towards the plant, 30-40cm away from the main stem on the sunny side of the host. The pit should be 40cm long, 30cm wide, and 45-55cm deep. Mix the original soil with well-rotted organic fertilizer and vermiculite to form a substrate. Sow 40% of the treated seeds on the sloping bottom of the pit, cover with 8-10cm of substrate, and then sow the remaining 60% of the seeds. Backfill the substrate to ground level and compact it. The seed quantity per pit is 1.8-2.2g. S4: Localized integrated water and fertilizer management in the root zone: Lay drip irrigation tape along the host plantation row, with the drippers facing the inoculation pit, buried 5-10cm deep; irrigate every 15-20 days in the early stage after inoculation, and every 25-30 days in the later stage, with a single irrigation volume of 20-30m³ per acre. 3 Apply mineral-derived potassium humate and high-phosphorus-potassium water-soluble fertilizer via drip irrigation 4-6 months after inoculation and in the following spring. S5: Harvesting and Host Care: Harvest 2-3 years after inoculation, before the Cistanche deserticola emerges from the soil or when it is just ≤5cm above the ground. Cut it 5-10cm above the swollen part connecting the root system. After harvesting, prune the Trichosanthes kirilowii and apply compound fertilizer.

[0007] Preferably, the specific specifications of the wide and narrow row planting pattern in step S1 are: wide row spacing of 4.0m, narrow row spacing of 1.0m, and plant spacing of 1.0m.

[0008] Preferably, the nurturing and management period in step S1 also includes: irrigating once every 15-20 days according to soil moisture, reducing irrigation after July according to rainfall, and carrying out winter irrigation in early to mid-November, while whitewashing the tree trunks or setting up fences.

[0009] Preferably, in step S2, the seeds must have a purity of ≥90% and a thousand-seed weight of ≥0.2g before treatment; the wet sand mixing ratio is seeds:sand = 1:3, and the sand moisture content is such that it can be formed into a ball by hand but crumbles easily when touched.

[0010] Preferably, in step S3, the inoculation pit wall is inclined at an angle of 30 degrees toward the plant, and the pit depth is 50 cm.

[0011] Preferably, the composition of the mixed substrate in step S3 is as follows: the original soil and decomposed organic fertilizer are mixed at a volume ratio of 5:1, and vermiculite or perlite accounting for 5% of the volume of the mixture is added.

[0012] Preferably, in step S4, the N-P2O5-K2O ratio of the high phosphorus and potassium water-soluble fertilizer is 10-30-20, and the application rate is 2-3 kg per mu; the application rate of mineral-derived potassium humate is 1 kg per mu.

[0013] Preferably, in step S4, the early stage after inoculation is within 2 months after inoculation, and the relative soil moisture content at the inoculation site is maintained at 60%-70%; the later stage is the growth and expansion period of Cistanche deserticola.

[0014] Preferably, after harvesting in step S5, the excavated pit is backfilled and compacted with the original soil, and overly dense and weak branches of the four-winged pheasant are pruned. A compound fertilizer with N-P2O5-K2O ratio of 15-15-15 is then applied once, at a rate of 10-15 kg per mu.

[0015] Preferably, it also includes green pest control steps: using black light lamps to attract and kill adult Cistanche deserticola borers, and spraying Bacillus subtilis to prevent root rot.

[0016] The present invention achieves the following beneficial technical effects compared to the prior art: This invention provides a highly efficient cultivation method for *Cistanche deserticola* inoculated with *Trichoderma tetrawingense*, characterized by standardized operation, uniform seed germination, high parasitism success rate, efficient water and fertilizer utilization, and significantly increased yield. Specifically, a composite seed treatment method combining low-temperature precooling, warm water soaking, and variable-temperature stratification, compared to single hormone soaking or low-temperature refrigeration, synergistically breaks the dormancy of *Cistanche deserticola* seeds, significantly improving seed germination potential and uniformity. By designing deep inoculation pits inclined towards the host root system, combined with a double-layer three-dimensional sowing pattern (bottom and middle layers) and backfilling with a nutrient substrate containing vermiculite, the *Trichoderma tetrawingense* roots are actively induced to grow directionally towards the seed-dense area, greatly expanding the parasitism interface and consistently increasing the inoculation success rate to over 95%. Furthermore, by precisely coupling the drip irrigation system with the location of the inoculation pit, integrated water and fertilizer management in the root zone is achieved. During the germination and swelling stages of Cistanche deserticola, mineral-derived potassium humate and high-phosphorus-potassium fertilizer are supplied in a targeted manner. This not only saves precious water resources in arid areas but also significantly promotes the accumulation of dry matter in Cistanche deserticola, resulting in a substantial increase in the yield of fresh Cistanche deserticola per mu. At the same time, the host plant care measures after harvesting ensure continuous production in the following year, ultimately achieving the organic unity of ecological restoration and high-value medicinal material production. Attached Figure Description

[0017] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0018] Figure 1 The flowchart illustrates the efficient cultivation method of Cistanche deserticola inoculated with Trichosanthes kirilowii provided by this invention. Detailed Implementation

[0019] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0020] The purpose of this invention is to provide a highly efficient cultivation method for Cistanche deserticola inoculated with Trichosanthes kirilowii, in order to solve the problems existing in the prior art.

[0021] To make the above-mentioned objects, features and advantages of the present invention more apparent and understandable, the present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments.

[0022] Example 1 This embodiment provides a highly efficient cultivation method for inoculating *Cistanche deserticola* with *Trichosanthes kirilowii*, such as... Figure 1 As shown, the specific steps are as follows.

[0023] Site Selection and Host Cultivation: At the experimental base located within the Baijitan National Nature Reserve in Lingwu City, Ningxia, a flat, well-drained sandy loam plot was selected. The soil salinity was measured at 0.4%, and the groundwater level was 2.0 meters. In early April, robust one-year-old *Acer tetragonum* seedlings were planted using a wide-narrow row planting pattern. The wide row spacing was 4.0 meters, the narrow row spacing was 1.0 meter, and the plant spacing was 1.0 meter. Planting holes were 40cm × 40cm × 40cm. During planting, ensure the roots were spread out, fill the soil in layers, and tamp it down. The planting depth was slightly deeper than the original root collar mark. Water thoroughly immediately after planting. During the first year of care after planting, drip irrigation was applied every 15 days according to soil moisture. Irrigation was stopped after July depending on rainfall, and a winter irrigation was carried out on November 15th. At the same time, the tree trunks are whitewashed before winter to prevent livestock from grazing on them and to protect them from damage by pikas and rabbits.

[0024] Compound treatment of Cistanche deserticola seeds: Cistanche deserticola seeds harvested in the current year were selected, with a purity of 92% and a thousand-seed weight of 0.22g. First, the seeds were pre-cooled at 4℃ for 8 days. After removal, they were soaked in 45℃ warm water for 24 hours, changing the water twice. After soaking, the seeds were mixed evenly with clean, moist sand at a volume ratio of 1:3. The sand moisture was controlled to a state where it could be formed into a clump by hand but crumbled easily upon light touch. The mixed seed-sand mixture was first warm-stratified at 25℃ for 12 days, then cold-stratified at 4℃ for 18 days to complete the treatment.

[0025] Directional three-dimensional inoculation: Inoculation was carried out in late April of the spring following the transplanting of *Trifolium repens*. An inoculation pit was dug 35 cm from the main stem on the west side of each *Trifolium repens* plant. The pit dimensions were 40 cm long, 30 cm wide, and 50 cm deep, with the walls sloping at a 30-degree angle towards the plant. The excavated soil was mixed with fully decomposed sheep manure at a 5:1 volume ratio, and vermiculite (5% by volume) was added and thoroughly mixed to prepare the nutrient substrate. First, 40% of the treated seeds were evenly sown on the sloping bottom of the pit, then covered with a 10 cm thick layer of nutrient substrate. Next, the remaining 60% of the seeds were evenly sown on this substrate layer. Finally, the pit was backfilled with the remaining mixed nutrient substrate until level with the ground, and then gently compacted. The total seed quantity per pit was 2.0 g. After backfilling and compaction, the pit was immediately and slowly watered thoroughly.

[0026] Integrated water and fertilizer management in the root zone: Within the narrow rows of planting rows of *Cistanche deserticola*, lay a drip irrigation tape along the planting row, adjusting the drippers to face the inoculation pits and burying them 8 cm deep underground. The first two months after inoculation constitute the early management stage, with drip irrigation every 15 days to maintain a relative soil moisture content of 65% at the inoculation pits. From the fourth month after inoculation to the following spring is the later management stage, when *Cistanche deserticola* enters the germination and swelling period; irrigation is then changed to once every 25 days, with a single irrigation volume of 25 cubic meters per mu (approximately 0.067 hectares). In the fifth month after inoculation, apply potassium humate (mineral-derived) with the irrigation water at a rate of 1 kg per mu. In April of the following spring, apply high-phosphorus and potassium water-soluble fertilizer (N-P2O5-K2O ratio of 10-30-20) with the irrigation water at a rate of 2.5 kg per mu.

[0027] Harvesting and Host Care: Two years and eight months after inoculation, in early April, when individual Cistanche deserticola plants have just emerged about 3 cm above the ground, harvest them. Carefully dig away the sandy soil and cut the fleshy stem 8 cm above the swollen area where the roots of Cistanche deserticola and Trichoderma tetrapanax are connected. After harvesting, backfill the pit with the original soil and compact it. Subsequently, prune the Trichoderma tetrapanax plants, removing overly dense, weak, and dead branches, and apply a 15-15-15 N-P2O5-K2O compound fertilizer at a rate of 12 kg per mu to restore tree vigor. Throughout the growing season, use black light traps to kill adult Cistanche deserticola borers, and spray with Bacillus subtilis wettable powder before the rainy season to prevent root rot.

[0028] Example 2 This embodiment is basically the same as Embodiment 1, except for adjustments to some technical parameters. In the planting site selection and host cultivation steps, the wide row spacing is 3.5 meters, the narrow row spacing is 1.2 meters, and the plant spacing is 1.2 meters. In the Cistanche deserticola seed compound treatment step, the low-temperature pre-cooling time is 10 days, the warm water soaking temperature is 40℃ for 24 hours, the warm stratification temperature is 22℃ for 14 days, and the cold stratification time is 16 days. In the directional three-dimensional inoculation step, the distance between the inoculation pit and the main stem is 32 cm, and the pit depth is 48 cm. In the root zone local water and fertilizer integrated management step, drip irrigation is performed once every 18 days in the early stage and once every 28 days in the later stage, with a single irrigation volume of 22 cubic meters per mu. The remaining steps and parameters are the same as in Embodiment 1.

[0029] Comparative Example 1 This comparative example uses the method described in Example 1 of patent application CN119836988A to inoculate *Cistanche deserticola* with *Trichoderma tetrawingense*. The specific steps are as follows: Select robust *Trichoderma tetrawingense* plants with well-developed root systems as host plants and plant them in desertified areas, providing adequate water using drip irrigation. Soak *Cistanche deserticola* seeds in a solution containing gibberellin for 18 hours. During the vigorous growth period of *Trichoderma tetrawingense* in spring, dig a small pit 8 cm deep at the base of the plant. Evenly sprinkle the inoculum, a mixture of *Cistanche deserticola* seeds and twice their weight of humus, into the pit, cover with soil and gently compact. Cover the seed surface with an 8 cm thick layer of fine sand, and cover the inoculation site with a plastic film. After inoculation, water every 2-3 days using a drip irrigation system, using 8 liters of water per plant each time, maintaining soil moisture at 60%-70%. Supplement with organic fertilizer as needed, and provide shade using a 40% shade net, removing weeds. When the Cistanche deserticola plant grows to 40 cm, it is harvested, washed, sliced ​​and dried.

[0030] Comparative Example 2 This comparative example is basically the same as Example 1, except for the seed treatment and inoculation methods. Seed treatment involved only 25 days of cold storage at 4°C, without hot water soaking or subsequent variable-temperature stratification. The inoculation method used conventional single-layer flat-bottomed pit sowing, i.e., digging a conventional flat-bottomed square pit (not inclined towards the plant), mixing all the seeds with the substrate, and then scattering them all at once into the bottom of the pit. After backfilling and compaction, layered sowing was not performed.

[0031] Performance testing and effect evaluation The cultivation methods of Examples 1-2 and Comparative Examples 1-2 were evaluated, and the seed germination rate, inoculation success rate, average yield per fresh Cistanche deserticola plant in the third year after inoculation, and content of effective components were statistically analyzed. Seed germination rate was measured in petri dishes, with the radicle breaking through the seed coat as the standard. Inoculation success rate was determined by randomly selecting 50 inoculation pits 18 months after inoculation and checking for newly formed Cistanche deserticola haustoria or tender fleshy stems. The total content of echinacoside and verbascoside was determined according to the methods in Part I of the 2020 edition of the Chinese Pharmacopoeia. Specific results are shown in Table 1.

[0032] Table 1 Comparison of the effects of different cultivation methods

[0033] As can be seen from the data in Table 1, the germination rate, inoculation success rate, single-plant yield, and content of effective medicinal components of Cistanche deserticola seeds obtained using the methods of Examples 1 and 2 of this invention are significantly better than those of Comparative Examples 1 and 2. Specifically, the seed germination rate of Example 1 is as high as 91.5%, while the germination rate of Comparative Example 1, which was treated with gibberellin, is only 68.4%. This indicates that the composite seed treatment method of this invention can more effectively break the dormancy of Cistanche deserticola seeds and promote their uniform germination. In terms of inoculation success rate, Example 1 reached 97.6%, while Comparative Example 1 was 81.5%, and Comparative Example 2 was even lower at 79.3%. This fully demonstrates that the directional inclined pit combined with double-layer three-dimensional sowing technology used in this invention effectively induces the host root system to grow towards the seed-dense area, greatly improving the parasitism probability. In terms of yield, the average yield per plant in Example 1 reached 1.85 kg, significantly higher than the 0.95 kg in Comparative Example 1 and 1.08 kg in Comparative Example 2, representing an increase of over 70%. This is attributed to the optimized integrated water and fertilizer management of this invention, which provides precise nutrient support for the later-stage expansion of Cistanche deserticola. Simultaneously, the higher content of active ingredients indicates that the method of this invention improves yield while also maintaining the intrinsic quality of the medicinal material. The above comparative results strongly demonstrate that the technical solution proposed in this invention has significant advantages and outstanding substantive features compared to existing technologies.

[0034] This invention has illustrated its principles and implementation methods using specific examples. The descriptions of these embodiments are merely illustrative of the method and its core ideas; furthermore, those skilled in the art will recognize that modifications may be made to the specific implementation methods and application scope based on the principles of this invention. Therefore, the content of this specification should not be construed as limiting the invention.

Claims

1. A highly efficient cultivation method for *Cistanche deserticola* inoculated with *Trigonella foenum-graecum*, characterized in that, Includes the following steps: S1: Site selection and host cultivation: Select sandy loam or light loam soil plots with a soil salinity of 0.2%-0.6% and a groundwater level below 1.5m; plant four-winged cattails using a wide-narrow row pattern, with a wide row spacing of 3-4m, a narrow row spacing of 1.0-1.5m, and a plant spacing of 1.0-1.5m. After planting, water thoroughly to settle the roots and carry out nurturing and management for no less than one year, during which winter irrigation is required; S2: Compound treatment of Cistanche deserticola seeds: Cistanche deserticola seeds are first pre-cooled at 4℃ for 7-10 days, then soaked in 40-45℃ warm water for 24 hours, and then mixed with wet sand, and successively stratified at 20-25℃ for 10-15 days and at 4℃ for 15-20 days. S3: Directional three-dimensional inoculation: In the spring or autumn of the year following the host plantation, dig an inoculation pit at a 30° angle towards the plant, 30-40cm away from the main stem on the sunny side of the host. The pit should be 40cm long, 30cm wide, and 45-55cm deep. Mix the original soil with well-rotted organic fertilizer and vermiculite to form a substrate. Sow 40% of the treated seeds on the sloping bottom of the pit, cover with 8-10cm of substrate, and then sow the remaining 60% of the seeds. Backfill the substrate to ground level and compact it. The seed quantity per pit is 1.8-2.2g. S4: Localized integrated water and fertilizer management in the root zone: Lay drip irrigation tape along the host plantation row, with the drippers facing the inoculation pit, buried 5-10cm deep; irrigate every 15-20 days in the early stage after inoculation, and every 25-30 days in the later stage, with a single irrigation volume of 20-30m³ per acre. 3 Apply mineral-derived potassium humate and high-phosphorus-potassium water-soluble fertilizer via drip irrigation 4-6 months after inoculation and in the following spring. S5: Harvesting and Host Care: Harvest 2-3 years after inoculation, before the Cistanche deserticola emerges from the soil or when it is just ≤5cm above the ground. Cut it 5-10cm above the swollen part connecting the root system. After harvesting, prune the Trichosanthes kirilowii and apply compound fertilizer.

2. The method for efficient cultivation of Cistanche deserticola by inoculating Trichosanthes kirilowii according to claim 1, characterized in that, The specific specifications for the wide and narrow row planting pattern in step S1 are: wide row spacing of 4.0m, narrow row spacing of 1.0m, and plant spacing of 1.0m.

3. The method for high-efficiency cultivation of Cistanche deserticola inoculated with Trichosanthes kirilowii according to claim 1, characterized in that, The nurturing and management period in step S1 also includes: irrigating once every 15-20 days according to soil moisture, reducing irrigation after July according to rainfall, and carrying out winter irrigation in early to mid-November, while whitewashing the tree trunks or setting up fences.

4. The method for efficient cultivation of Cistanche deserticola by inoculating Trichosanthes kirilowii according to claim 1, characterized in that, In step S2, the seeds must have a purity of ≥90% and a thousand-seed weight of ≥0.2g before treatment. The wet sand mixing ratio is 1:3 (seeds:sand). The sand moisture content should be such that it can be formed into a ball by hand but crumbles easily when touched.

5. The method for efficient cultivation of Cistanche deserticola by inoculating Trichosanthes kirilowii according to claim 1, characterized in that, In step S3, the inoculation pit wall is inclined at an angle of 30 degrees toward the plant, and the pit depth is 50cm.

6. The method for high-efficiency cultivation of Cistanche deserticola inoculated with Trichosanthes kirilowii according to claim 1, characterized in that, The composition of the mixed substrate in step S3 is as follows: the original soil and decomposed organic fertilizer are mixed at a volume ratio of 5:1, and vermiculite or perlite accounting for 5% of the volume of the mixture is added.

7. The method for efficient cultivation of Cistanche deserticola inoculated with Trichosanthes kirilowii according to claim 1, characterized in that, In step S4, the N-P2O5-K2O ratio of the high phosphorus and potassium water-soluble fertilizer is 10-30-20, and the application rate is 2-3 kg per mu; the application rate of mineral-derived potassium humate is 1 kg per mu.

8. The method for efficient cultivation of Cistanche deserticola by inoculating Trichosanthes kirilowii according to claim 1, characterized in that, In step S4, the early stage after inoculation is within 2 months after inoculation, maintaining the relative soil moisture content at the inoculation site at 60%-70%; the later stage is the growth and expansion period of Cistanche deserticola.

9. The method for efficient cultivation of Cistanche deserticola inoculated with Trichosanthes kirilowii according to claim 1, characterized in that, After harvesting in step S5, backfill the excavation pit with the original soil and tamp it down. Prune the overly dense branches and weak branches of the four-winged pheasant, and apply a compound fertilizer with N-P2O5-K2O ratio of 15-15-15, at a rate of 10-15 kg per mu.

10. The method for efficient cultivation of Cistanche deserticola by inoculating Trichosanthes kirilowii according to claim 1, characterized in that, It also includes green prevention and control measures for diseases and pests: using black light lamps to attract and kill adult borers of Cistanche deserticola, and spraying Bacillus subtilis to prevent root rot.

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