A composition, its preparation and use for enhancing liposome penetration

Abstract

This application discloses a composition and its preparation and application of permeation-enhancing liposomes, belonging to the field of cosmetic production technology. The technical solution is as follows: a composition composed of Forsythia suspensa extract, Lysimachia christinae extract and Sophora flavescens root extract, wherein the mass ratio of Forsythia suspensa extract, Lysimachia christinae extract and Sophora flavescens root extract is 2-8:0.5-3:2-6. The composition provided by this application can scavenge free radicals and reduce oxidative damage. In the experiment, the above substances produced a good synergistic effect when used in combination.

Description

Technical Field

[0001] This application belongs to the field of cosmetic manufacturing technology, and more specifically, relates to a composition and the preparation and application of its permeation-enhancing liposomes. Background Technology

[0002] With the fast pace of life and increasing environmental pollution, skin faces increasingly severe oxidative stress challenges. Exogenous factors such as ultraviolet radiation, particulate matter, and free radicals in the air can easily lead to lipid peroxidation of skin cell membranes, collagen degradation, and DNA damage, thereby accelerating the skin aging process and manifesting as fine lines, age spots, and decreased elasticity. To combat this process, the development of highly effective compositions has become an important direction in cosmetic science.

[0003] However, currently available antioxidants (such as vitamin C, vitamin E, and polyphenols) generally face the following technical bottlenecks:

[0004] Unstable activity: Vitamin C (ascorbic acid) is easily oxidized and deactivated by light, oxygen and metal ions, resulting in a short product shelf life and reduced antioxidant effect after use.

[0005] Poor skin permeability: The stratum corneum of the skin has a significant barrier function, and many highly effective antioxidants, due to their large molecular weight or strong polarity, have difficulty penetrating the skin to reach the dermis and exert their effects.

[0006] Lack of synergistic effect: The antioxidant effect of a single ingredient is limited, and the synergistic mechanism between different antioxidants has not been fully explored, resulting in the overall antioxidant capacity being lower than expected.

[0007] Chinese patent application 201610039654.4 discloses a Dendrobium officinale frozen mask, which is made from the following raw materials in parts by weight: 30-50 parts of polymer, 10-15 parts of water, 30-40 parts of moisturizer, 50-60 parts of Dendrobium officinale extract, 5-10 parts of Bletilla striata extract, 2-5 parts of Poria cocos extract, 1-3 parts of Sophora flavescens root extract, 4-8 parts of aloe vera extract, 10-15 parts of seaweed extract, and 5-8 parts of Forsythia suspensa extract.

[0008] Further examination of its instructions reveals that Sophora flavescens is cold in nature and has the effects of clearing heat and drying dampness. As a plant-based herbal medicine, it can balance oil secretion, unclog and tighten pores, remove toxins and impurities from the skin, and has rich herbal nutrients. It can promote the growth and repair of damaged blood vessel nerve cells, restore the vitality of subcutaneous capillary cells, and make the skin firm and smooth again, thus playing a role in beauty and skin care. Forsythia suspensa is bitter and cool in nature and has the effects of clearing heat and detoxifying, dispersing nodules and reducing swelling, and draining pus.

[0009] It is evident that the Forsythia suspensa extract and Sophora flavescens root extract in the above scheme tend to have a repairing effect rather than an antioxidant effect.

[0010] In addition, although plant extracts have gradually become a research hotspot for active ingredients in cosmetics in recent years, the effects of combining different plant extracts are difficult to predict due to their wide variety.

[0011] Therefore, the actual technical problem to be solved by this application is: how to provide a composition obtained by compounding multiple plants. Summary of the Invention

[0012] The main objective of this application is to provide a composition obtained by combining multiple plants, and to further enhance the overall antioxidant capacity of the composition through the synergistic effect between multiple plant extracts.

[0013] To achieve the above objectives, this application provides a composition comprising Forsythia suspensa extract, Lysimachia christinae extract and Sophora flavescens root extract, wherein the mass ratio of Forsythia suspensa extract, Lysimachia christinae extract and Sophora flavescens root extract is 2-8:0.5-3:2-6.

[0014] Preferably, it also includes a root extract of Ampelopsis japonica, wherein the mass ratio of the Forsythia suspensa extract, the Rhizoma Sedum sarmentosum extract, the root extract of Sophora flavescens, and the root extract of Ampelopsis japonica is 2-8:0.5-3:2-6:0.5-3.

[0015] In this application, Forsythia extract can directly scavenge reactive oxygen species (ROS), such as DPPH and superoxide anion free radicals, and protect cells from oxidative damage;

[0016] The ethyl acetate extract of *Gnaphalium affine* has a strong scavenging ability against DPPH free radicals and also has a certain iron ion reducing ability.

[0017] The alkaloids in Sophora flavescens (such as matrine and oxymatrine) have certain antioxidant activity, which can scavenge free radicals and reduce oxidative damage.

[0018] The root extract of *Ampelopsis japonica* is rich in polyphenols, which also have good free radical scavenging ability. Furthermore, during the experiment, the above substances produced a good synergistic effect when used in combination.

[0019] Furthermore, this application also discloses the use of the composition described above in the preparation of liposomes.

[0020] In addition, this application also discloses a permeation-enhancing liposome comprising 1 to 30 wt% of the composition described above.

[0021] Preferably, the liposomes are obtained by homogenization of the composition, sunflower seed oil, olive fruit oil, squalane, water, glycerol and phospholipids;

[0022] The mass ratio of the composition, sunflower seed oil, olive fruit oil, squalane, water, glycerin and phospholipids is 3-10:3-6:3-6:5-15:15-40:5-40:3-5.

[0023] Preferably, the phospholipid is selected from at least one of hydrogenated lecithin, soybean lecithin, and lecithin.

[0024] Preferably, the method for preparing the liposomes includes the following steps:

[0025] Step 1: Stir and mix the composition, sunflower seed oil, olive fruit oil, squalane and water at 80-85°C to obtain an intermediate;

[0026] Step 2: Heat the glycerol hydrogenated lecithin to 70-80°C, then add it to the intermediate and homogenize it at 5000-6000 rpm for 3-5 minutes. After cooling the material to room temperature, shear homogenize the material through a microfluidic high-pressure homogenizer at 25000-30000 psi to obtain liposomes.

[0027] In addition, this application also discloses an essence lotion containing the aforementioned penetration-enhancing liposomes.

[0028] One of the above-mentioned technical solutions in this application has at least one of the following advantages or beneficial effects: the Forsythia suspensa extract in the composition disclosed in this application can directly scavenge reactive oxygen species (ROS), such as DPPH and superoxide anion free radicals, and protect cells from oxidative damage; the ethyl acetate of the Sophora flavescens extract has a strong scavenging ability against DPPH free radicals and also has a certain iron ion reducing ability; the alkaloid components in Sophora flavescens (such as matrine and oxymatrine) have certain antioxidant activity, can scavenge free radicals, reduce oxidative damage, and in the experiment, the above substances produced a good synergistic effect when used in combination. Attached Figure Description

[0029] Figure 1 This is a picture of a chicken embryo before the test in Example 1;

[0030] Figure 2 These are images of chicken embryos after the chicken embryo test in Example 1. Detailed Implementation

[0031] The present application will be clearly and completely described below with reference to its embodiments. It should be noted that, unless specific conditions are specified in the embodiments, conventional conditions or conditions recommended by the manufacturer shall apply. Reagents or instruments whose manufacturers are not specified are all commercially available conventional products.

[0032] The following examples and the raw materials and their suppliers involved in subsequent experiments are shown in Table 1:

[0033] Table 1: Raw Material Source Information

[0034] Raw material name supplier Forsythia extract Bozhou Yuanshengtang Pharmaceutical Sales Co., Ltd. Falling Bells Extract Bozhou Yuanshengtang Pharmaceutical Sales Co., Ltd. White peony root extract Bozhou Yuanshengtang Pharmaceutical Sales Co., Ltd. Sophora flavescens root extract Bozhou Yuanshengtang Pharmaceutical Sales Co., Ltd.

[0035] Examples 1-6

[0036] A composition is prepared by weighing the plant extracts according to their mass fractions, then stirring and mixing them to obtain the composition. The types and mass fractions of the plant extracts in each embodiment are shown in Table 2.

[0037] Table 2: Formulation Table for Examples 1-6

[0038] Group Forsythia extract Falling Bells Extract Sophora flavescens root extract White peony root extract Example 1 2 3 2 0 Example 2 4 2 4 0 Example 3 8 0.5 6 0 Example 4 4 2 4 0.5 Example 5 4 2 4 2 Example 6 4 2 4 3

[0039] Comparative Examples 1-4

[0040] The preparation method is the same as in Example 1, except that the types and mass fractions of plant extracts in each comparative example are shown in Table 3:

[0041] Table 3: Formulation Tables for Comparative Examples 1-4

[0042] Group Forsythia extract Falling Bells Extract Sophora flavescens root extract White peony root extract Comparative Example 1 4 0 4 0 Comparative Example 2 0 2 0 0 Comparative Example 3 4 0 4 2 Comparative Example 4 0 2 0 2

[0043] Performance testing

[0044] 1. Stimulation test

[0045] Test method: BWEL-SPF fertilized chicken embryos from the SPF experimental animal center of Xinxing Dahua Agricultural Poultry Egg Co., Ltd. were selected. The chicken embryos met the requirements of the relevant standard GB / T17999.1-2008.

[0046] Ten chickens were selected for each test. The condition of the chorioallantoic membrane was recorded by photographing. A polytetrafluoroethylene (PTFE) resin ring was placed on the chorioallantoic membrane of the chicken embryo, and the image was taken. The test sample (the sample prepared in Example 1 was diluted with pure water to a mass fraction of 3%) was added to the PTFE resin ring, and the time of sample addition was recorded. The air cell was then covered with a moistened plastic wrap and placed in a constant temperature and humidity incubator for incubation. The degree of change in toxicity effects was observed.

[0047] Observe and record the vascular effects classification, and score them according to their severity. The scoring criteria are shown in Table 4.

[0048] Table 4: Chicken Embryo Testing Scoring Criteria

[0049] score Observation description No response 0 No reaction was observed; CAM is normal. Ghost Veins 1 There is no blood flow inside the blood vessels, and the blood vessels are clearly visible. Capillary damage 2 Congestion, or increased blood flow to CAM small vessels. Very minor bleeding 3 There are a few tiny bleeding points (1-10) inside the ring. minor bleeding 4 There are more than 10 countable small bleeding points inside the ring, or the small bleeding points are uncountable and cover less than 1 / 8 of the area inside the ring. Very mild bleeding 5 The area covered by the bleeding points within the ring is 1 / 8 to 1 / 4, or there are no bleeding points within the ring but the area covered by the red halo is less than 1 / 4. Mild bleeding 6 The area covered by the bleeding points within the ring is 1 / 4 to 1 / 2, or there are no bleeding points within the ring but the area covered by the red halo is 1 / 4 to 1 / 2. Moderate bleeding 7 Numerous bleeding points or small areas of bleeding within the ring cover 1 / 2 to 3 / 4 of the ring. Severe bleeding 8 Bleeding points within the ring cover more than 3 / 4 of the area; crusting may form.

[0050] The test indicators are shown in Table 5:

[0051] Table 5: Criteria for Judging Stimuli Testing

[0052] Test indicators Judgment criteria CAMVA index 0 < NC ≤ 2, stress stimulus; 2 < NC ≤ 3, mild stimulus; 3 < NC ≤ 5, moderate stimulus, NC > 5, severe stimulus

[0053] The test results are shown in Table 6:

[0054] Table 6: Stimulation test results of Example 1

[0055] Serial Number Sample Name Number of experimental chicken embryos Effective number of chicken embryos Overall score Test results 1 Negative control (0.9% saline) 3 3 0 0.00 2 Positive control (10% acrylamide) 3 3 14 4.67 3 Sample 1 (The sample obtained in Example 1 was diluted with pure water to a mass fraction of 3%) 10 8 12 1.50

[0056] Results analysis:

[0057] Refer to Table 6 and Figure 1-2 It is evident that the composition obtained in Example 1 is not irritating when diluted to 3 wt%.

[0058] 2. DPPH free radical scavenging rate / %

[0059] Test methods

[0060] (1) Reagents

[0061] Unless otherwise specified, all reagents used are of analytical grade, and the water is Grade I water as specified in GB / T6682.

[0062] 95% ethanol;

[0063] 1,1-Diphenyl-2-trinitrophenylhydrazine (Sigma);

[0064] 0.12 mg / L DPPH ethanol solution: Weigh 12 mg of 1,1-diphenyl-2-trinitrophenylhydrazine into a 250 ml beaker, add 100 ml of 95% ethanol, and stir with a glass rod until dissolved.

[0065] Positive control: Vitamin E, purity ≥96%.

[0066] (2) Experimental methods

[0067] The positive control was dissolved and diluted with 95% ethanol to prepare a series of concentration gradients of 0.08 mg / mL, 0.04 mg / mL, 0.02 mg / mL, and 0.01 mg / mL to verify the test system.

[0068] Test substance treatment: The test substance was diluted with pure water to a 2% aqueous solution.

[0069] Set up sample tubes (T), sample background (T0), DPPH tubes (C), and solvent background (C0) using 10mL test tubes. For each sample, three parallel tubes should be set up for each test concentration of the sample tube (T), and three parallel tubes should also be set up for the DPPH tube (C). Add 1L of sample solution of the same concentration to each of the sample tubes (T) and sample background (T0).

[0070] Add solvent to all test tubes (T, T0, C, C0), using water for water-soluble samples and 95% ethanol for oil-soluble samples, to a total of 3 mL, and mix well.

[0071] Add 1 mL of DPPH ethanol solution to the sample tube (T) and DPPH tube (C), and replace the sample background (T0) and solvent background (C0) with 95% ethanol. Shake gently and let stand at room temperature for 5 minutes.

[0072] Each reaction solution was transferred into a 1 cm cuvette, and the absorbance was measured at 517 nm.

[0073] (3) Calculation of results

[0074] The formula for calculating the DPPH free radical scavenging rate is shown in Equation 1:

[0075] Formula 1

[0076] (1) In the formula:

[0077] T—Absorbance of the sample tube, i.e., absorbance of the solution after the sample reacts with DPPH;

[0078] T0—Sample background absorbance;

[0079] The average of three absorbance values ​​of the C-DPPH tube, i.e. the absorbance of the DPPH solution without the addition of sample;

[0080] C0 — Solvent background absorbance.

[0081] The test results are shown in Table 7:

[0082] Table 7: Performance Test Results of Examples and Comparative Examples

[0083] Group DPPH free radical scavenging rate / % Group DPPH free radical scavenging rate / % Example 1 35.763 Example 6 53.064 Example 2 39.546 Comparative Example 1 28.976 Example 3 32.147 Comparative Example 2 20.758 Example 4 43.970 Comparative Example 3 26.943 Example 5 58.315 Comparative Example 4 19.018 Blank control 10.216

[0084] Results analysis:

[0085] 1. As can be seen from Table 7, when the mass ratio of Forsythia suspensa extract, Lysimachia christinae extract and Sophora flavescens root extract was slightly adjusted in Examples 1-3, the free radical scavenging ability of Examples 1-3 fluctuated to some extent, but overall it remained at a level greater than 30%.

[0086] 2. Furthermore, when the root extract of Ampelopsis japonica was added to Examples 4-6 based on Example 2, the free radical scavenging ability of Examples 4-6 was significantly improved compared to Example 2. It can be seen that the addition of Ampelopsis japonica root extract can further enhance the antioxidant capacity of the composition.

[0087] 3. Observation of Comparative Examples 1-2 shows that when Comparative Example 1 uses Forsythia suspensa and Sophora flavescens root extracts to prepare the composition, the free radical scavenging rate of Comparative Example 1 is only 28.976%, while when Comparative Example 2 uses only Sophora flavescens extract, the DPPH free radical scavenging rate of Comparative Example 2 is only 20.758%. However, observation of Example 2 shows that its free radical scavenging rate reaches 39.546%, which is much higher than either of Comparative Examples 1-2. This shows that Sophora flavescens extract has a synergistic effect with Forsythia suspensa and Sophora flavescens root extracts, which significantly improves the antioxidant capacity of the composition.

[0088] 4. Observation of Comparative Examples 3-4 shows that, firstly, the antioxidant capacity of Comparative Example 2 and Comparative Example 4 is similar, and Comparative Example 4 is even lower than that of Comparative Example 2.

[0089] The antioxidant capacity of Comparative Example 1 and Comparative Example 3 is similar, with Comparative Example 3 being slightly weaker than Comparative Example 1.

[0090] Therefore, it can be seen that when the extract of Ampelopsis japonica root is used together with the extract of Lysimachia christinae or when the extract of Ampelopsis japonica root is used together with the extracts of Forsythia suspensa and Sophora flavescens root, there is no synergistic effect or the synergistic effect is weak.

[0091] Furthermore, observations of Comparative Examples 3, 4, and 5 show that when all four extracts were used in Example 5, its antioxidant capacity was increased by approximately 18% compared to Example 2.

[0092] Based on the previous analysis, it can be inferred that when the extract of Ampelopsis japonica root was used together with the extract of Lysimachia christinae, there was no good synergistic effect between the two. Similarly, when the extract of Ampelopsis japonica root was used together with the extracts of Forsythia suspensa and Sophora flavescens root, there was no good synergistic effect.

[0093] When extracts of *Lysimachia christinae*, *Forsythia suspensa*, and *Sophora flavescens* are added to the composition, extract of *Ampelopsis japonica* root can exert a good synergistic effect with them.

[0094] Application Example 1

[0095] A permeation-enhancing liposome, prepared by the following method:

[0096] Sunflower seed oil, olive fruit oil, squalane, water, and the composition prepared in Example 6 were mixed and dissolved in proportion, and then heated to 85°C. Glyceryl hydrogenated lecithin was separately mixed in proportion and stirred until dissolved at 80°C. This solution was then added to the above mixture, and homogenized at 6000 rpm for 5 minutes until the mixture was smooth and homogeneous. Preservatives were then added and stirred until homogenized. After cooling to room temperature, the mixture was sheared and homogenized at 30000 psi using a microfluidic high-pressure homogenizer (the mixture was passed through the microfluidic homogenizer three times) to obtain the permeation-enhancing liposomes.

[0097] Furthermore, it should be noted that the amount of raw materials added in the above application example 1 is shown in Table 8:

[0098] Table 8: Formulation Table for Application Example 1

[0099] name Proportion (mass fraction) glycerin 35% Sunflower (Helianthus annuus) seed oil 5% Olive (Olea Europaea) fruit oil 5% squalane 10% Hydrogenated lecithin 4% The composition obtained in Example 6 6% p-Hydroxyacetophenone (preservative) 0.5% 1,2-Hexanediol (preservative) 0.5% water Add total to 100%

[0100] Permeability test

[0101] Using retinol as the target active ingredient, liposomes were diluted with deionized water to a mass percentage of 2%, and then formulated with retinol at a ratio of 95:5 to prepare the test sample, thereby examining the penetration-enhancing effect of the penetration-enhancing composition.

[0102] The transdermal absorption test was conducted using a pigskin-Franzcell permeation diffusion cell (the instrument was a TK type transdermal absorption tester; please refer to the instrument operation instructions), in accordance with the national standard GB / T27818-2011.

[0103] The specific method is as follows: Fresh pig back skin was cleaned by removing hair and subcutaneous tissue, dried, and cut into appropriate sizes for later use. The pig back skin came from a slaughterhouse. The pig back skin was fixed between the supply and receiving chambers of a TK type transdermal absorption tester. The receiving solution was isotonic PBS buffer, and the test solution was liposomes prepared in the application example. Five parallel test groups were conducted, and the average value was taken. The transdermal test was performed in a 37℃ circulating water bath. 0.5 mL samples were taken at 4 h and 12 h after the start of the test, and 0.5 mL of PBS was added after sampling. The samples were filtered through a 0.22 μm filter membrane and used as test samples. The diffusion percentage of retinol in the samples at 4 h and 12 h was detected and calculated. The retinol content was tested according to the determination method of "Guangdong Provincial Cosmetic Society Group Standard T / GDCA 005-2021 Retinol, Retinyl Acetate, Retinyl Palmitate, Hydroxypinazone Retinate in Cosmetics". The test results are shown in Table 9.

[0104] Table 9: Results of Liposome Permeation Enhancement Test

[0105] Percentage of retinol diffusion over 4 hours / % Percentage of retinol diffusion over 12 hours / % 2wt% Permeation-enhancing liposome aqueous solution 22.58 32.21 Blank (pure water) 5.62 7.68

[0106] Results analysis:

[0107] As can be seen from Table 9, the liposomes prepared using Example 1 can enhance the diffusion effect of retinone in the skin and have good penetration-enhancing ability.

[0108] Application Example 2

[0109] An essence emulsion, prepared by the following method:

[0110] Pre-phase 1: Dissolve a small amount of deionized water and arginine evenly for later use.

[0111] Pre-phase 2: Dissolve glycerol and p-hydroxyacetophenone completely in a water bath at 70°C with stirring.

[0112] Add deionized water to a beaker, then add trehalose, sodium hyaluronate, butylene glycol, glycerin, carbomer, nicotinamide, and disodium EDTA in sequence. Heat to 80-85℃ and stir at 9000 rpm for 15 minutes until homogeneous. In another beaker, add sorbitan stearate, polysorbate-60, PEG-100 stearate, glyceryl stearate, isopropyl myristate, triethylhexanoate, polydimethylsiloxane, tocopheryl acetate, and cetearyl alcohol in sequence. Heat to 80-82℃ and stir until completely dissolved.

[0113] The oil phase was added to the aqueous phase and homogenized at 3000 rpm for 5 minutes to emulsify. After emulsification, the mixture was cooled down. When the mixture cooled to 45°C, pre-phase 1, pre-phase 2, 1,2-hexanediol, and the permeation-enhancing liposomes prepared in Example 1 were added in sequence and stirred evenly.

[0114] In addition, it should be noted that the amount of material added according to the principle in Application Example 2 is shown in Table 10:

[0115] Table 10: Formulation Table for Application Example 2

[0116] Raw material name Proportion (mass fraction) Sorbitan stearate 0.9% Polysorbate-60 1.1% PEG-100 stearate (50%), glyceryl stearate (50%) 1.5% Isopropyl myristate 3% Glyceryl triethylhexanoate 3% polydimethylsiloxane 1% Tocopheryl acetate 0.1% Cetearyl alcohol 0.9% Trehalose 2% Sodium hyaluronate 0.02% Butylene glycol 6% glycerin 4% Carbomer 0.2% Arginine 0.2% Niacinamide 1% Disodium EDTA 0.02% p-Hydroxyacetophenone 0.5% 1,2-Hexanediol 0.5% Application of the permeation-enhancing liposomes prepared in Example 1 2% water Add total to 100%

[0117] Moisturizing efficacy test:

[0118] Principle: The capacitance method for determining the moisture content of the stratum corneum of human skin is based on the significant difference in dielectric constant between water and other substances. The measured capacitance value varies depending on the moisture content of the stratum corneum, and this parameter can represent the skin's moisture content. The test results are shown in Table 11.

[0119] Table 11: Moisturizing Effect Test Table for Application Example 2

[0120]

[0121] Results analysis:

[0122] As can be seen from the test results in Table 11, after using the essence prepared in Example 2 of this application, the MMV value of the subject's skin surface was significantly increased compared with the blank control group, indicating that the essence lotion of this application has a good moisturizing effect.

Claims

1. A composition characterized in that, It is composed of Forsythia suspensa extract, Lysimachia christinae extract and Sophora flavescens root extract, wherein the mass ratio of Forsythia suspensa extract, Lysimachia christinae extract and Sophora flavescens root extract is 2-8:0.5-3:2-6.

2. The composition according to claim 1, characterized in that, It also includes a root extract of Ampelopsis japonica, wherein the mass ratio of the Forsythia suspensa extract, the Rhizoma Sedum sarmentosum extract, the root extract of Sophora flavescens, and the root extract of Ampelopsis japonica is 2-8:0.5-3:2-6:0.5-3.

3. Use of the composition according to any one of claims 1-2 in the preparation of liposomes.

4. A permeation-enhancing liposome, characterized in that, It includes 1 to 30 wt% of the composition as described in any one of claims 1-2.

5. The permeation-enhancing liposome according to claim 4, characterized in that, The liposomes were obtained by homogenization of the composition, sunflower seed oil, olive fruit oil, squalane, water, glycerol and phospholipids; The mass ratio of the composition, sunflower seed oil, olive fruit oil, squalane, water, glycerin and phospholipids is 3-10:3-6:3-6:5-15:15-40:5-40:3-5.

6. The permeation-enhancing liposome according to claim 5, characterized in that, The phospholipid is selected from at least one of hydrogenated lecithin, soybean lecithin, and lecithin.

7. The permeation-enhancing liposome according to claim 4, characterized in that, The method for preparing the liposomes includes the following steps: Step 1: Stir and mix the composition, sunflower seed oil, olive fruit oil, squalane and water at 80-85°C to obtain an intermediate; Step 2: Heat glycerol and hydrogenated lecithin to 70-80°C, then add them to the intermediate and homogenize at 5000-6000 rpm for 3-5 minutes. After cooling the material to room temperature, shear homogenize the material using a microfluidic high-pressure homogenizer at 25000-30000 psi to obtain liposomes.

8. An essence lotion, characterized in that, It contains the permeation-enhancing liposomes as described in any one of claims 4-7.

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