A poly-T double-stranded chimeric DNA-copper nanoparticle and its synthesis method

By designing DNA tetrahedral templates with specific sequences to synthesize DNA-copper nanoparticles, the problem of the single function of DNA-CuNPs was solved, and copper nanoparticles with both fluorescence properties and peroxidase-like activities were realized for synergistic effects in bacterial detection and removal.

CN122298972APending Publication Date: 2026-06-30SHAANXI NORMAL UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SHAANXI NORMAL UNIV
Filing Date
2026-04-03
Publication Date
2026-06-30

AI Technical Summary

Technical Problem

In the existing technology, the function of DNA-CuNPs is mainly focused on fluorescence properties, with little research on their enzyme-like activities. Furthermore, there are no reports of synthesizing copper nanoparticles with both fluorescence and peroxidase-like activities using poly-T-double-stranded chimeric DNA tetrahedra as templates.

Method used

Using a DNA tetrahedral template designed with a specific sequence, copper ions are reduced in situ with ascorbic acid to prepare poly-T double-stranded chimeric DNA-copper nanoparticles. With specific structural optimization, these nanoparticles acquire fluorescence properties and peroxidase-like activity for bacterial detection and removal.

Benefits of technology

The synthesized DNA-copper nanoparticles exhibit characteristic fluorescence under 340 nm excitation, which can be used for quantitative detection of bacteria. They can also catalyze the decomposition of hydrogen peroxide without the addition of hydrogen peroxide, thereby achieving efficient bacterial removal. They have good reproducibility and potential for large-scale application.

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Abstract

This invention discloses a poly-T double-stranded chimeric DNA-copper nanoparticle and its synthesis method, belonging to the field of DNA-metal nanomaterials technology. This invention uses a poly-T double-stranded DNA chimeric structure as a template to construct a DNA tetrahedron. Copper ions are reduced in situ with ascorbic acid to prepare DNA-copper nanoparticles exhibiting both fluorescence and peroxidase-like activity. The DNA tetrahedron template is formed by the self-assembly of four single-stranded DNA strands, containing six double-stranded edges and four single-stranded vertices. Two of the single strands are poly-T sequences, and two are aptamer sequences targeting bacterial cell wall peptidoglycan. The prepared DNA-copper nanoparticles emit characteristic fluorescence under 340 nm excitation and exhibit peroxidase-like activity. This material is used for bacterial detection and eradication, with minimum inhibitory concentrations (MICs) of 25 nM and 5 nM against *Escherichia coli* and methicillin-resistant *Staphylococcus aureus*, respectively. 3 Up to 10 8 The ability to detect Escherichia coli within the CFU / L concentration range demonstrates its potential for immediate clinical antibacterial applications.
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