A strain of swine lactobacillus HL1 from wild boar in xinjiang region and application of feed additive thereof

Abstract

This invention belongs to the field of microbiology technology, and specifically relates to the application of a strain of *Lactobacillus suis* HL1 derived from wild boars in Xinjiang and its feed additives. Lactobacillus saerimneri HL1, with accession number CGMCC No. 34386. This invention relates to *Lactobacillus suis* HL1, which exhibits good tolerance, strong antioxidant capacity, and effective antibacterial properties. It is sensitive to multiple antibiotics and promotes growth and development, enhancing intestinal villi growth, increasing nutrient absorption, and improving coat luster. This application isolates a potentially beneficial *Lactobacillus* strain with low or no drug resistance from wild boars in Xinjiang and evaluates its in vivo safety, laying the foundation for animal feed additives.

Description

Technical Field

[0001] This invention belongs to the field of microbiology technology, and in particular relates to the application of a strain of Mythical Lactobacillus suis HL1 from wild boars in Xinjiang and its feed additives. Background Technology

[0002] Since adding antibiotics to feed can lead to antibiotic residues and bacterial resistance, probiotics, as live microorganisms, can stimulate the intestinal flora, change the gastrointestinal environment, maintain the health of the host, and perform beneficial physiological functions during the breeding process.

[0003] Existing research indicates that commonly used probiotic strains include yeast, Bacillus, and lactic acid bacteria. These strains are widely used at different stages of piglet production, and can prevent and alleviate intestinal diseases, improve growth performance, lower cholesterol levels, and reduce antibiotic use.

[0004] Lactic acid bacteria (LAB) are the most common probiotics and are widely used in animal feed. They can regulate the balance of animal intestinal flora, inhibit the growth of pathogenic bacteria, promote the richness and diversity of intestinal flora, improve nutrient absorption, and enhance immunity.

[0005] Existing studies have demonstrated that *Lactobacillus acidophilus*, *Lactobacillus plantarum*, and *Lactobacillus salivarius* can all improve the growth performance of organisms and can replace antibiotic growth promoters. Currently, there are many studies on isolating lactic acid bacteria from livestock, poultry, and dairy products, but fewer reports on isolating lactic acid bacteria from wild boars. Summary of the Invention

[0006] This application isolates potentially beneficial lactobacilli with low or no drug resistance from wild boars in Xinjiang and evaluates their in vivo safety, laying the foundation for feed additives for domestic pigs.

[0007] This invention provides a *Lactobacillus suis* strain derived from wild boars in Xinjiang. Lactobacillus saerimneri HL1, accession number: CGMCC No.34386.

[0008] This invention provides a *Lactobacillus suis* strain derived from wild boars in Xinjiang. Lactobacillus saerimneri Application of HL1 in the preparation of feed additives.

[0009] This invention provides a *Lactobacillus suis* strain derived from wild boars in Xinjiang. Lactobacillus saerimneri Application of HL1 in the preparation of antioxidant products.

[0010] This invention provides a *Lactobacillus suis* strain derived from wild boars in Xinjiang. Lactobacillus saerimneri Application of HL1 in the preparation of antibacterial agents.

[0011] This invention provides a *Lactobacillus suis* strain derived from wild boars in Xinjiang. Lactobacillus saerimneri Application of HL1 in the preparation of growth-promoting probiotic formulations.

[0012] Furthermore, the antibacterial species are Escherichia coli, Staphylococcus aureus, and Salmonella typhimurium.

[0013] Furthermore, the antibiotics include β-lactams, chloramphenicols, cephalosporins, macrolides, carbapenems, quinolones, anesamycins, and lincomycins.

[0014] This invention provides a growth-promoting probiotic preparation containing *Lactobacillus mythosti* derived from wild boars in Xinjiang. Lactobacillus saerimneri HL1.

[0015] Compared with the prior art, the present invention has the following beneficial effects: The Mythical Lactobacillus HL1 of this invention exhibits good tolerance, strong antioxidant capacity, and effective antibacterial properties. It is sensitive to a variety of antibiotics and promotes growth and development by increasing intestinal villi growth and nutrient absorption.

[0016] 1. This invention is the first to isolate and identify Mythical Lactobacillus HL1 from wild boars in Xinjiang. Screening has confirmed that it has strong inhibitory ability against common diarrheal bacterial pathogens and is sensitive to a variety of antibiotics.

[0017] 2. Mythical Lactobacillus HL1 can promote the growth and development of experimental animals. Compared with the blank group, the low-dose group significantly increased the average daily weight gain (ADG), serum IGF-1 content, and IGF-1 gene expression in the liver.

[0018] 3. This invention is the first to discover that the screened Mythical Lactobacillus HL1 can significantly increase the length of the jejunal villi in experimental animals, enhance nutrient absorption, and improve the condition of the animal's coat. It has important developmental research value and can be developed into a probiotic preparation that promotes growth.

[0019] Biological Preservation Instructions: Preservation institution: China General Microbiological Culture Collection Center, China Committee on the Preservation and Management of Microbial Cultures; Accession number: CGMCC No. 34386; Deposit date: April 28, 2025; Address: No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing; Taxonomic nomenclature: Lactobacillus saerimneri HL1. Attached Figure Description

[0020] Figure 1 This is a morphological diagram of the strain isolated in Example 1; Figure 2 Image of Gram-positive bacilli from Example 1; Figure 3 This is a diagram showing the 16S rRNA gene amplification results in Example 1; Figure 4 This is the phylogenetic tree in Example 1; Figure 5 This is a growth curve of *Lactobacillus mythologius* HL1 in Example 1; Figure 6 This is the acid production curve of *Lactobacillus mythologius* HL1 in Example 1; Figure 7 The hemolytic activity of *Lactobacillus mythologius* HL1 in Example 1; Figure 8 The hemolytic activity of Staphylococcus aureus in Example 1; Figure 9 HE staining of liver and spleen tissue sections from Experiment 1. Liver (10×): CV (central vein), HL (liver lobule); Spleen (10×): WP (white pulp), RP (red pulp); A: (liver) Control, B: (liver) HL1-H, C: (liver) HL1-M, D: (liver) HL1-L. E: (spleen) Control, F: (spleen) HL1-H, G: (spleen) HL1-M, H: (spleen) HL1-L. Figure 10 The content of IGF-1 in the serum of mice in Experiment Example 1; Figure 11 The gene expression level of IGF-1 in the liver of mice in Experiment Example 1; Figure 12 The expression level of the PI3K gene in the liver of mice in Experiment Example 1; Figure 13 The gene expression level of AKT in the liver of mice in Experiment Example 1; Figure 14 The expression level of mTOR gene in the liver of mice in Experiment Example 1; Figure 15 HE staining images of jejunal tissue sections from Experiment Example 2; where A is Control, B is HL1-H, C is HL1-M, and D is HL1-L; Figure 16 The effect of *Lactobacillus suis* HL1 on intestinal structure was investigated in Experiment 2. Figure 15 Jejunal villus height; data are expressed as mean ± standard deviation (mean ± SD) (*** P <0.001); Figure 17 The effect of *Lactobacillus suis* HL1 on the weight of weaned piglets in Experiment Example 3; Figure 18 The effect of *Lactobacillus mythologius* HL1 on the coat of weaned piglets was shown in Experiment Example 3. Detailed Implementation

[0021] Example 1 Sample source and preliminary screening of strains, strain identification and probiotic characteristics 1. Sample source and preliminary screening of strains Ten samples of wild boar feces were collected from Changji and Hami areas of Xinjiang. Each sample was placed in a 50mL sterile centrifuge tube containing 30mL glycerol, labeled, and placed in a cold chain container before being quickly transported back to the laboratory. 0.1g of wild boar feces was placed in a 1.5mL sterile centrifuge tube, and 1mL of sterile physiological saline was added and vortexed to mix. 100μL of the supernatant was added to 900μL of MRS broth and incubated anaerobicly at 37℃ for 24h. The bacterial culture was then diluted to 10⁻⁶. -5 10 -6 and 10 -7 The culture was spread on MRS agar medium and anaerobically cultured at 37°C upside down for 24 hours. Different morphological strains were picked and repeatedly streaked for purification until colonies with consistent morphology and size were isolated. After Gram staining, the colonies were examined under a microscope to screen for positive bacilli. The bacterial culture was then mixed with glycerol at a volume ratio of 1:1 and stored at -40°C.

[0022] like Figure 1 As shown, single colonies of the isolated strain on MRS solid medium appear as milky white, round, slightly convex in the center, with a smooth surface and irregular edges; like Figure 2 As shown, the bacterial cell morphology is that of Gram-positive bacilli.

[0023] 2. Identification of strains Biochemical identification: The isolated strains were identified using HBI lactic acid bacteria biochemical identification strips (purchased from Qingdao Haibo Biotechnology, China), and the identification results were compared with those in Bergey's Manual of Systematic Bacteria.

[0024] Molecular biological identification: Bacterial DNA was extracted by boiling in water and amplified using universal bacterial primers 27F (5′-AGAGTTTGATCCTGGCTCAG-3′) and 1492R (5′-GGTTACCTTGTTACGACTT-3′). The reaction conditions were: 94℃ for 3 min; 94℃ for 30 s, 53℃ for 30 s, 72℃ for 1 min, for a total of 30 cycles; 72℃ extension for 5 min; 4℃ ∞.

[0025] like Figure 3 As shown, the size of the product fragment after amplification of the isolated strain is approximately 1500 bp; like Figure 4 As shown, the sequencing results were compared on NCBI, and a phylogenetic tree was constructed to identify the isolated strain as... Lactobacillus saerimneri (Mythical Lactobacillus suis), named HL1.

[0026] 3. Growth curve and acid production curve of the strain Strain strain HL1 was inoculated into MRS broth medium at a 2% inoculation rate, and an appropriate amount of bacterial solution was taken every 2 hours to measure the absorbance (OD). 600nm ( ) and pH value and plot the growth curve.

[0027] like Figure 5 As shown, when Lactobacillus mythosus HL1 was inoculated into MRS broth, the strain entered the logarithmic growth phase after 2 hours and entered the stationary phase after 18 hours. like Figure 6 As shown, the pH value decreased to below 4.0 after 16 hours and reached its lowest value after 24 hours.

[0028] 4. Acid and bile salt resistance tests The overnight culture was inoculated at a rate of 10% into MRS broth with a pH of 3.0 and a bile salt concentration of 0.3%, with MRS broth (pH 5.7) serving as a control.

[0029] Incubate at 37℃ for 3 hours, and calculate the survival rate using the plate count method.

[0030] The calculation formula is as follows: Survival rate (%) = (lgA1 / lgA2) × 100%, Wherein: A1 is MRS broth containing hydrochloric acid (PH3) and bile salts (0.3%), and A2 is MRS broth.

[0031] As shown in Table 1, the survival rate of *Lactobacillus mythologius* HL1 after culturing for 3 hours at pH 3.0 was 98.66%, while the survival rate after culturing for 3 hours at 0.3% bile salts was 36.48%. Compared with *Lactobacillus plantarum* isolated from *Fragrant Pig* feces samples by Chen Suilian et al. (Isolation, Identification and Biological Characteristics Study of a Lactic Acid Bacterium from Fragrant Pigs [J]. Feed Industry, 2024, 45(10):113-118.), *Lactobacillus mythologius* HL1 has stronger acid and bile salt tolerance.

[0032] Table 1 Results of acid and bile salt resistance tests on Mythical Lactobacillus HL1

[0033] 5. Self-agglomeration experiment Take 1 mL of the overnight culture and add it to a 1.5 mL sterile centrifuge tube. Centrifuge at 10000 rpm for 10 min, discard the supernatant, wash twice with PBS, and resuspend in 4 mL PBS. Measure the OD. 600nmThe absorbance value (A3) was measured at 37℃. The incubator was incubated for 16h and 20h, and the OD value of the supernatant was measured. 600nm (A4).

[0034] The calculation formula is as follows: Self-agglomeration rate (%) = [(A3-A4) / A3] × 100%.

[0035] As shown in Table 2, the autoagglutination rates of *Lactobacillus mythologius* HL1 after 16 h and 20 h of culture were 83.23% and 84.77%, respectively. Compared with *Enterococcus montmorilloni* M6-5 isolated and identified from wild boar feces samples in the Daxinganling region of Heilongjiang Province by Wang Yi et al. (Isolation, identification and characteristics of lactic acid bacteria in wild boar feces [J]. Chinese Agricultural Science, 2020, 53(14):2964-2973), *Lactobacillus mythologius* HL1 has a stronger autoagglutination ability.

[0036] Table 2 Results of the autoagglutination rate test of Lactobacillus suis HL1

[0037] 6. Surface hydrophobicity test Take 1 mL of the overnight culture and add it to a 1.5 mL sterile centrifuge tube. Centrifuge at 10000 rpm for 10 min, discard the supernatant, wash twice with PBS, and resuspend in 4 mL PBS. Measure the OD. 600nm The absorbance value was measured at A5. Xylene (analytical grade reagent, 100% concentration) was added at a 1:1 volume ratio, shaken for 5 min, and incubated at 37℃ for 4 h. The aqueous phase was carefully aspirated, and the OD was measured. 600nm (A6).

[0038] The calculation formula is as follows: Hydrophobicity (%) = [(A5-A6) / A5] × 100%.

[0039] As shown in Table 3, the hydrophobicity of *Lactobacillus mythologica* HL1 after 4 hours of culture was 71.59%. Compared with *Lactobacillus mythologica* GLP236 isolated and identified by Zhang Yuanyuan et al. (Isolation, identification, in vitro probiotic function and safety evaluation in mice of *Pediococcus canis* GLP236 [J]. Chinese Journal of Animal Nutrition, 2023, 35(12): 8148-8160), *Lactobacillus mythologica* HL1 has stronger hydrophobicity.

[0040] Table 3. Hydrophobicity test results of Mythical Lactobacillus HL1

[0041] 7. Antioxidant Experiment Take 1 mL of the overnight cultured bacterial suspension, centrifuge at 7000 rpm for 5 min, discard the supernatant, wash twice with PBS, and resuspend in an equal volume of PBS. Add 1 mL each of 0.1 mmol / L DPPH-anhydrous ethanol solution (purchased from Shanghai Yisheng Biotechnology Co., Ltd.) and the resuspended bacterial suspension to a 5 mL sterile centrifuge tube, vortex to mix, and incubate at room temperature in the dark for 30 min. Then, aspirate 200 μL of the solution into a sterile 96-well plate and measure the OD. 517nm (A7); Use anhydrous ethanol solution instead of DPPH-anhydrous ethanol solution to determine OD. 517nm (A8); Use PBS solution instead of resuspended bacterial culture for OD determination. 517nm (A9).

[0042] The calculation formula is as follows: Clearance rate (%) = [1-(A7-A8) / A9]×100%.

[0043] Centrifuge the overnight cultured bacterial suspension at 7000 rpm for 5 min, discard the supernatant, wash twice with PBS, and resuspend in an equal volume of PBS. Add 1 mL each of 7 mmol / L ABTS solution (purchased from Shanghai Yisheng Biotechnology Co., Ltd.) and the resuspended bacterial suspension to a 5 mL sterile centrifuge tube, vortex to mix, and incubate at room temperature in the dark for 5 min. Then, aspirate 200 μL of the solution into a sterile 96-well plate and measure the OD. 734nm (A) 10 ); Use sterile deionized water instead of ABTS solution to determine OD. 734nm (A) 11 ); Use PBS solution instead of resuspended bacterial culture for OD measurement. 517nm (A) 12 The calculation formula is as follows: Clearance rate (%) = [1 - (A)] 10 -A 11 ) / A 12 ]×100%.

[0044] As shown in Table 4, compared with Lactobacillus reuteri T-B5L2 isolated and identified from Tibetan pig feces by Fan Qiuyue et al. (Isolation, identification and probiotic characteristics determination of lactic acid bacteria from Tibetan pigs [J]. Acta Microbiologica Sinica, 1-14), Lactobacillus mythologica HL1 has stronger antioxidant capacity.

[0045] Table 4 Results of the free radical scavenging test of Mythical Lactobacillus HL1

[0046] 8. Antibacterial test The Oxford cup agar diffusion method was used. 1 mL of bacterial suspension after 18 h of culture was centrifuged at 6000 rpm for 5 minutes and filtered through a 0.2 nm filter to obtain cell-free supernatant. The bacterial pellet was resuspended in an equal volume of PBS. Sterilized Oxford cups were placed on solid culture media coated with pathogenic bacterial suspensions (Escherichia coli, Staphylococcus aureus, and Salmonella typhimurium; the pathogens were spread onto solid culture medium, then placed in the Oxford cup, and bacterial suspension and supernatant were added). 200 μL each of overnight cultured bacterial suspension, cell-free supernatant, and bacterial suspension were added. The mixture was incubated at 37°C for 24 h, and the diameter of the inhibition zone was measured using calipers. Three replicates were performed.

[0047] Table 5 shows that the bacterial suspension and cell-free supernatant of *Lactobacillus mythologius* HL1 inhibited *Escherichia coli*, *Staphylococcus aureus*, and *Salmonella*. Wang Yi et al. (Isolation, Identification and Characterization of Lactic Acid Bacteria in Wild Boar Feces [J]. Chinese Agricultural Science, 2020, 53(14):2964-2973) isolated and identified *Enterococcus montelukastiensis* M6-5 from wild boar feces samples in the Daxinganling region of Heilongjiang Province, which only inhibited *Escherichia coli* and *Salmonella typhimurium*. *Lactobacillus mythologius* HL1 was able to inhibit a wider range of pathogenic bacteria.

[0048] Table 5 Results of antibacterial test of Lactobacillus suis HL1

[0049] 9. Drug sensitivity test The disk diffusion method was used, and 10 commonly used test drugs for lactic acid bacteria were selected according to ISO 10932 / IDF 233 standards (as shown in Table 6). 100 μL of the test bacterial culture after 18 hours of overnight incubation was spread onto an MRS plate, and different types of antibiotic disks were placed on top. The plate was incubated at 37°C for 24 hours, and the diameter of the inhibition zone was measured using calipers. Three replicates were performed.

[0050] Table 6 shows that *Lactobacillus mythologica* HL1 was resistant to tetracycline and erythromycin, but sensitive to the remaining eight antibiotics. Compared with *Lactobacillus mythologica* GLP236 isolated and identified by Zhang Yuanyuan et al. (Isolation, identification, in vitro probiotic function and safety evaluation in mice of *Pediococcus canis* GLP236 [J]. Chinese Journal of Animal Nutrition, 2023, 35(12): 8148-8160), *Lactobacillus mythologica* HL1 showed fewer resistant species and was safer.

[0051] Table 6. Results of Antimicrobial Susceptibility Testing for Lactobacillus suis HL1

[0052] Note: R indicates resistance to the antibiotic, and S indicates sensitivity to the antibiotic.

[0053] 10. Hemolysis test The bacterial suspension incubated overnight for 18 hours and the Staphylococcus aureus bacterial suspension (positive control) were inoculated separately (bacterial suspension OD). 600 (nm=1.67, streak the inoculation loop) onto a blood agar plate, incubate anaerobically at 37°C for 48 hours, and observe whether a hemolytic ring forms.

[0054] like Figure 7 As shown, *Lactobacillus mythologius* HL1 exhibits γ-hemolysis on blood agar plates. like Figure 8 As shown, Staphylococcus aureus exhibits β-hemolysis on blood agar plates.

[0055] Experimental Example 1 Effects of oral administration of Mythical Lactobacillus HL1 on growth performance and organs in mice Fifty-six Kunming mice (half male and half female), weighing (27±2) g, were randomly divided into four groups of 14 mice each after acclimatization for 7 days. The control group was administered 0.2 mL of sterile saline by gavage, while the high-dose, medium-dose, and low-dose groups were administered 4.2 × 10⁻⁶ mL of sterile saline by gavage. 10 CFU / mL, 4.2×10 9 CFU / mL, 4.2×10 8 0.2 mL of the isolated strain at a concentration of CFU / mL.

[0056] Mice were administered the medication via gavage for 27 consecutive days. Daily body weight and feed intake were recorded, and average daily gain (ADG), average daily feed intake (ADFI), and feed conversion ratio (F / A) were calculated. On day 28, the liver and spleen of the mice were dissected. The calculation formula is as follows: Organ coefficient (%) = (organ weight / mouse body weight) × 100, and tissue sections were prepared for HE staining. Blood was collected using the ocular blood collection method and collected in ordinary blood collection tubes. The serum was collected by centrifugation at 3000 rpm and 4℃ for 15 min. The content of insulin-like growth factor (IGF-1) in the serum was detected using an enzyme-linked immunosorbent assay kit, strictly following the instructions.

[0057] The expression levels of IGF-1, PI3K, AKT, and mTOR in the liver at the mRNA level were detected using real-time quantitative polymerase chain reaction (qPCR).

[0058] As shown in Table 7, the final body weight and average daily weight gain of mice in the low-dose group were significantly higher than those in the control group (P < 0.05); the average daily feed intake and feed conversion ratio of mice in the low-dose group were better than those in the control group, but there were no significant differences (P > 0.05).

[0059] Table 7. Effects of Mythical Lactobacillus HL1 on growth performance in mice.

[0060] As shown in Table 8, the liver coefficient of the control group mice was significantly higher than that of the high-dose group mice (P < 0.05), but there was no significant difference compared with the low-dose and medium-dose groups mice (P > 0.05). Compared with the control group, the spleen coefficient of the experimental group mice was not significantly different (P > 0.05).

[0061] Table 8. Effects of Mythical Lactobacillus HL1 on Mouse Organs

[0062] like Figure 9 As shown, no obvious pathological changes were observed in the liver and spleen tissue sections of the four groups of mice. like Figure 10 As shown, by detecting the IGF-1 content in the serum of Kunming mice, it was found that the IGF-1 content in the serum of mice in the low-dose group was significantly higher than that in the control group (P<0.05), while there was no significant difference in the content among the remaining three groups. Depend on Figure 11 It can be seen that by detecting the levels of IGF-1, PI3K, AKT and mTOR in the liver of Kunming mice using qPCR technology, the gene expression of IGF-1 in the HL1-L group was significantly higher than that in other groups. Depend on Figure 12 It can be seen that the gene expression of PI3K in the HL1-L group was significantly higher than that in the HL1-H group; Depend on Figure 13 It can be seen that the AKT gene expression in the HL1-H, HL1-M, and HL1-L groups was significantly higher than that in the control group; Depend on Figure 14 It can be seen that there was no significant difference in mTOR gene expression levels among HL1-H, HL1-M, HL1-L1 and the blank group, while gene expression levels in the HL1-L group showed an increasing trend. Gavage administration of *Lactobacillus suis* HL1 can promote the secretion of IGF-1, regulate the classical PI3K / AKT / mTOR growth pathway, and promote the growth of the organism.

[0063] Experiment Example 2 Effect of oral administration of Mythical Lactobacillus HL1 on jejunal villus length in mice After feeding using the method in Experiment Example 1, tissue sections were prepared and stained with hematoxylin and eosin (HE).

[0064] The jejunal villus length was measured using DAS software, and finally plotted and analyzed using GraphPad.

[0065] like Figure 15As can be seen from HE staining of jejunum sections, the jejunum structure of the four groups of mice is obvious, the mucosal epithelium is intact, it is a single layer of columnar epithelium, the villi are relatively dense and slender and finger-like, and the central lacteals can be clearly seen; the small intestinal glands in the lamina propria are neatly arranged, and a small number of goblet cells can be seen.

[0066] like Figure 16 As shown in the left figure, by measuring the height of the small intestinal villi, this invention found that the villi height in the HL1-L group was significantly higher than that in the Control group, HL1-H group, and HL1-M group (P<0.001). Gavage administration of HL1 to Mythical Pigs can promote jejunal villi growth and increase nutrient absorption.

[0067] Experimental Example 3 The effects of feeding Mythical Lactobacillus HL1 on the weight and coat condition of weaned piglets were investigated. The activated bacterial suspension HL1 was inoculated onto MRS solid medium and anaerobically cultured at 37°C for 24 h. Single colonies were picked and anaerobically cultured overnight at 37°C in MRS broth. After centrifugation at 7000 rpm for 5 min, the supernatant was discarded, and an equal volume of freeze-drying protectant (10% skim milk powder + 0.1% L-glutamate monosodium salt + 0.05% L-cysteine) was added for resuspending. The mixture was then poured into freeze-drying containers and pre-frozen overnight at -40°C before freeze-drying. Weaned piglets in the control group were fed a basal diet, while those in the freeze-dried group were fed a diet containing 0.1% freeze-drying protectant and basal diet. Weaned piglets in the bacterial group were fed a diet containing 0.1% freeze-dried bacterial powder and basal diet. The final live bacteria count in the feed was 3.6 × 10⁶. 8 CFU / g. Piglets were fed continuously for 28 days, and their weight was recorded every 7 days. The coat condition of piglets in the control group and the experimental group was also observed.

[0068] like Figure 17 It was found that the weight gain of weaned piglets in the Lactobacillus HL1 group was significantly higher than that in the control group after 28 days of continuous feeding. Feeding Lactobacillus HL1 can promote the growth of weaned piglets.

[0069] like Figure 18 It was found that the coats of weaned piglets fed with Mythical Lactobacillus HL1 were smoother and shinier than those of piglets in other groups. Feeding Mythical Lactobacillus HL1 can improve the coat and hair quality of animals.

[0070] The embodiments described above are merely preferred embodiments of the present invention and are not intended to limit the scope of the present invention. Various modifications and improvements made by those skilled in the art to the technical solutions of the present invention without departing from the spirit of the present invention should fall within the protection scope defined by the claims of the present invention.

Claims

1. A type of *Lactobacillus suis* derived from wild boars in Xinjiang (… Lactobacillus saerimneri HL1, accession number: CGMCC No.34386.

2. A *Lactobacillus suis* strain from wild boars in Xinjiang, as described in claim 1. Lactobacillus saerimneri Application of HL1 in the preparation of feed additives.

3. A *Lactobacillus suis* strain from wild boars in Xinjiang, as described in claim 1. Lactobacillus saerimneri Application of HL1 in the preparation of antioxidant products.

4. A *Lactobacillus suis* strain from wild boars in Xinjiang, as described in claim 1. Lactobacillus saerimneri Application of HL1 in the preparation of antibacterial agents.

5. A *Lactobacillus suis* strain from wild boars in Xinjiang as described in claim 1 (…). Lactobacillus saerimneri Application of HL1 in the preparation of growth-promoting probiotic formulations.

6. A *Lactobacillus suis* strain from wild boars in Xinjiang, as described in claim 1. Lactobacillus saerimneri Application of HL1 in improving animal coat condition.

7. The application as described in claim 4, characterized in that, The bacteria inhibited are Escherichia coli, Staphylococcus aureus, and Salmonella typhimurium.

8. The application as described in claim 5, characterized in that, The antibiotics include β-lactams, chloramphenicols, cephalosporins, macrolides, carbapenems, quinolones, anesamycins, and lincomycins.

9. A growth-promoting probiotic preparation, characterized in that, Contains the Mythical Lactobacillus suis from wild boars in Xinjiang as described in claim 1. Lactobacillus saerimneri i HL1.

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