A method and application for nicotine conversion based on a co-immobilized multi-enzyme cascade system

By using a co-immobilized multi-enzyme cascade system, the problems of enzyme stability and coenzyme efficiency in the nicotine conversion process were solved, achieving efficient and stable conversion of nicotine to 3-succinylpyridine, which is suitable for the resource utilization of tobacco waste.

CN122303349APending Publication Date: 2026-06-30GANSU TOBACCO IND +1
View PDF 0 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
GANSU TOBACCO IND
Filing Date
2026-04-01
Publication Date
2026-06-30

AI Technical Summary

Technical Problem

Existing nicotine conversion methods suffer from problems such as insufficient enzyme stability, high cost of coenzymes, and lack of spatial organization among multiple enzymes, making it difficult to achieve efficient and green conversion of nicotine to 3-succinylpyridine. Furthermore, both whole-cell systems and single-enzyme catalytic systems have limitations.

Method used

A co-immobilized multi-enzyme cascade system was adopted, in which nicotine dehydrogenase, pseudooxynicotine amine oxidase, 3-succinyl hemialdehyde pyridine dehydrogenase and aldehyde-ketone reductase were co-immobilized on the surface of streptavidin agarose microspheres using AviTag tags and SpyCatcher/SpyTag technology, forming a multi-enzyme cascade system to achieve multi-step continuous conversion of nicotine, and the reaction conditions were optimized by coenzyme regeneration through AKR modules.

Benefits of technology

It achieves highly selective accumulation of nicotine to 3-succinylpyridine, reduces the amount of coenzyme supplementation, improves the conversion rate and terminal yield, and the multi-enzyme catalyst maintains high stability over a wide pH range and high temperature, making it suitable for repeated use.

✦ Generated by Eureka AI based on patent content.

Smart Images

  • Figure CN122303349A_ABST
    Figure CN122303349A_ABST
Patent Text Reader

Abstract

This invention relates to a method and application of nicotine conversion based on a co-immobilized multi-enzyme cascade system. The method includes the following steps: adding a co-immobilized multi-enzyme catalyst to a reaction system containing nicotine; the co-immobilized multi-enzyme catalyst includes nicotine dehydrogenase carrying an AviTag tag, pseudooxynicotine amine oxidase, 3-succinyl hemialdehyde pyridine dehydrogenase – SpyCatcher, and aldehyde-ketone reductase – SpyTag; in NADP... + The reaction was carried out in a buffer solution in the presence of a sacrificial substrate; after the reaction, the reaction solution was separated and purified to obtain the product 3-succinylpyridine. This invention significantly reduces NADP. + The coenzyme dosage was adjusted, and the co-immobilized multi-enzyme catalyst exhibited excellent pH and thermal stability as well as recyclability, achieving efficient and targeted conversion of nicotine. This provides a new technical approach for the resource utilization of nicotine from tobacco waste and the green preparation of 3-succinylpyridine.
Need to check novelty before this filing date? Find Prior Art