Method for rapidly screening high-yield strain of antifungal active substance HSAF
By combining small-dose fermentation broth extraction and microplate fermentation with specific assay bacteria culture and plate confrontation detection, the problems of long screening cycle and high cost of high-yield HSAF strains have been solved, and a high-efficiency and low-cost screening process has been achieved.
CN122303370APending Publication Date: 2026-06-30NANJING UNIV OF SCI & TECH +1
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Patent Information
- Application Number
- CN202610478340.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Priority Date
- 2025-12-31
- Filing Date
- 2026-04-13
- Publication Date
- 2026-06-30
AI Technical Summary
Technical Problem
Existing technologies for screening high-yield HSAF strains are characterized by long screening cycles, high costs, and low efficiency. Traditional methods are also subject to blindness and safety risks, making them unsuitable for large-scale mutant library screening.
Method used
By employing methods such as small-dose fermentation broth extraction, microplate fermentation, specific assay culture, and plate confrontation detection, and by optimizing the fermentation system and detection process, high-yield HSAF strains can be efficiently screened.
Benefits of technology
It significantly improves screening throughput and detection speed, reduces costs, is easy to operate, is suitable for large-scale mutation library screening, and improves screening efficiency and accuracy.
✦ Generated by Eureka AI based on patent content.
Abstract
The invention discloses a method for rapidly screening a high-yield strain of an antifungal active substance HSAF. According to the method, small-dose extraction, micro-pore plate high-throughput fermentation, specific verification bacterium culture and plate confrontation detection are integrated. High-flux fermentation of strains is realized through a 48-hole square hole plate, and the flux is increased by dozens of times compared with that of a traditional shake flask; according to the method, colletotrichum orbiculare is selected as specific verification bacteria, spore suspension liquid with the concentration of 1 * 10 CFU / mL is prepared, a clear inhibition zone is formed within 12 hours based on a plate confrontation method, rapid screening of high-yield strains is completed, finally, HSAF is extracted with a small dosage of 1 mL of fermentation liquor, and the reagent consumption is reduced by 60% or above compared with that of a conventional method. The method is simple and convenient to operate and good in repeatability, is an ARTP mutagenesis and high-yield HSAF strain integrated screening technology established for lysobacter enzymogenes OH11 for the first time at home and abroad, and provides a key technical support for industrialization of HSAF.
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