A method for arterial perfusion of venous tissue from cadaver specimens based on printing ink and an automated arteriovenous differential colorimetric perfusion reagent.

Abstract

This invention relates to the field of medical research based on cadaver specimens, specifically disclosing a method for arterial perfusion of veins from cadaver specimens based on ink and an automated arteriovenous differential colorimetric perfusion reagent. The perfusion reagent includes ink and a base perfusion solution, wherein the base perfusion solution is a latex, gelatin solution, or other base perfusion solution that can pass through the capillary network and does not affect the color-changing effect of the ink. This invention innovatively discovers that the perfusion solution containing ink undergoes a color change as it flows from the artery into the vein. Utilizing this principle, a one-step method for arterial perfusion of veins from cadaver specimens is provided. This method is simple to operate, requiring only one artery puncture and one perfusion solution, and is less likely to cause dilation or deformity of the vein after perfusion. The anatomical field of view is clear, and perfusion can be terminated when the perfusion solution mixture flows out of the vein. After perfusion, the artery and vein exhibit different colors, and the color difference is visually discernible, thus achieving differential display of arteries and veins.

Description

Technical Field

[0001] This invention relates to the field of medical research based on cadaver specimens (forensic medicine, medical anatomy research, surgical training research, etc.), specifically, to a method for arterial perfusion of venipuncture in cadaver specimens based on printing ink and an automated arteriovenous differential colorimetric perfusion reagent. Background Technology

[0002] Vascular perfusion involves injecting a colored perfusion solution into blood vessels, allowing for visualization of vascular location, course, and branching characteristics through dissection and other methods. There are two main existing methods for cadaveric venous perfusion: the first involves directly perfusing a mixture of perfusion solution and dye after multiple punctures in peripheral veins; the second involves injecting a mixture of one color of perfusion solution and dye into an artery until it flows out of the vein, then continuing perfusion with a mixture of another color for a period of time. Colored dyes used for perfusion include red and blue ink (organic dyes) and mercury pigment. The direct perfusion method involving multiple punctures in peripheral veins is cumbersome, makes it difficult to perfuse all veins, and often results in dilated and deformed veins, even obscuring accompanying arteries, increasing the difficulty of dissection and reducing visual clarity. The second method requires two perfusions with two different colored solutions. Furthermore, since it is impossible to objectively determine whether the second colored perfusion solution-dye mixture reaches the capillary network, the time for terminating the perfusion cannot be determined. As a result, the perfusion effect often manifests as bicolor perfusion in arteries or veins.

[0003] Chinese Patent 2023118066074 discloses a combined formulation for combined arteriovenous vascular imaging in mice, comprising formulation A (gelatin microparticles + pigment) and formulation B (latex microparticles + pigment). Formulation A is perfused first, followed by formulation B. The gelatin microparticles are small enough to pass through capillaries into the venous end, while the latex microparticles are larger and can only reach small arteries, not the venous end. After perfusion and curing, the color of the mouse arteries and veins can be used to distinguish them. Chinese Patent 2024119029715 discloses a postmortem arteriovenous differential angiography method, in which contrast material X is perfused into the arterial system of the cadaver, and contrast material Y is perfused into the venous system. Separate perfusion of arteries and veins allows for the generation of independent venous and arterial images using subtraction techniques. While the above-mentioned prior arteries have improved the perfusion solution or imaging scheme, they essentially employ a two-stage perfusion method, requiring multiple perfusions to achieve differential visualization of arteries and veins. No prior art has yet disclosed a method that achieves differential visualization of arteries and veins through a single arterial puncture and one-step perfusion.

[0004] Ink refers to the oily material used to color stamps, enabling them to leave marks on paper or other materials. Ink formulations mainly include pigments, resins, and carrier solvents. Pigments are the color-developing raw materials for ink, commonly using organic pigments such as scarlet, golden red, phthalocyanine black, and phthalocyanine blue. The pigment content generally accounts for 5-30% of the total weight of the ink formulation. Resins are binders, commonly including acrylic resins, alkyd resins, and polyamide resins, used to fix the pigments to the substrate surface. The resin content generally accounts for 15%-55% of the total weight of the ink formulation. Carrier solvents are used to dissolve or disperse the pigments and impart appropriate fluidity and permeability to the ink. Common carrier solvents are alcohols or other organic solvents, and the carrier solvent content generally accounts for 30-80% of the total weight of the ink formulation. In addition, some atomic ink formulations may include small amounts of dispersants, stabilizers, anti-settling agents, etc. (usually 0.1-5%) to prevent pigment settling and optimize the uniformity of ink dispersion. Patent applications CN911107171, CN921134320, CN931036364, etc., disclose the formula of atomic printing ink, which has been disclosed in existing literature. Summary of the Invention

[0005] The first objective of this invention is to address the shortcomings of the prior art by providing a one-step arterial perfusion automated differential colorimetric perfusion reagent for arteriovenous infusion.

[0006] The second objective of this invention is to address the shortcomings of the prior art by providing the use of printing ink in the preparation of automated arteriovenous differential colorimetric perfusion reagents.

[0007] The third objective of this invention is to address the shortcomings of the prior art by providing a method for arterial perfusion of venipuncture into cadaver specimens based on printing ink.

[0008] To achieve the first objective mentioned above, the technical solution adopted by the present invention is as follows:

[0009] A one-step arterial perfusion method for automatically differentially developing colorimetric perfusion reagent via arterial perfusion, wherein the perfusion reagent includes printing ink, and the volume ratio of the printing ink to the total volume of the perfusion reagent is 1:(151~351).

[0010] In the one-step arterial perfusion automated differential colorimetric perfusion reagent described above, the preferred ratio of the printing oil volume to the total volume of the perfusion reagent is 1:(151~251).

[0011] In the one-step arterial perfusion automated differential colorimetric perfusion reagent described above, the perfusion reagent specifically includes a basal perfusion solution that can pass through a capillary network.

[0012] In the one-step arterial perfusion automated differential colorimetric perfusion reagent described above, the base perfusion solution is further defined as latex, gelatin solution, or other base perfusion solutions that can pass through the capillary network and do not affect the color-changing effect of the printing ink.

[0013] To achieve the second objective mentioned above, the technical solution adopted by the present invention is as follows:

[0014] The use of printing ink in the preparation of automated arteriovenous differential colorimetric perfusion reagent.

[0015] In the application described above, the further preparation refers to mixing the printing ink with the base injection fluid.

[0016] In the applications described above, the base perfusion fluid is further described as latex, gelatin solution, or other base perfusion fluid that can pass through the capillary network and does not affect the ink color-changing effect.

[0017] In the application described above, the volume ratio of the printing ink to the base injection fluid is further described as 1:(150~350).

[0018] To achieve the third objective mentioned above, the technical solution adopted by the present invention is as follows:

[0019] A method for perfusion of a cadaveric specimen artery with an ink-based perfusion agent, comprising the step of perfusing the cadaveric specimen artery with the perfusion agent as described above.

[0020] The method described above further includes a step of stopping the infusion after the infusion reagent flows out of the venous end.

[0021] The advantages of this invention are:

[0022] 1. The present invention provides a method for perfusing veins through arteries in cadaver specimens. This method is based on a one-step perfusion technique, which is simple, easy to perform, and highly reproducible. Differentiated color development can be achieved by using different brands of printing ink in experiments.

[0023] 2. In this invention, the mixture of perfusion fluid and printing oil enters along the artery, passes through the capillary network, and enters the venous system, theoretically capable of perfusing all venous vessels.

[0024] 3. In this invention, the flow direction of the mixture of perfusion fluid and printing ink is artery-capillary network-vein. After the perfusion fluid-dye mixture passes through the capillary network, the perfusion pressure decreases, which can avoid excessive dilation of veins and help maintain the vascular morphology.

[0025] 4. The method of the present invention can realize the dual-color display of arteries and veins in cadaver specimens. The color display effect is intuitive and can be clearly identified with the naked eye, which is convenient for teaching and anatomical observation.

[0026] 5. This invention does not limit the type of basic perfusion fluid, only requiring that it can pass through the capillary network; in addition to the latex and gelatin solutions listed in the examples, other commonly used perfusion fluids are applicable. Therefore, this invention has a wide range of applications, low implementation costs, and is easy to promote.

[0027] In summary, this invention innovatively discovers that the perfusion fluid containing printing ink undergoes a color change as it flows from the artery into the vein. Utilizing this principle, a one-step method for perfusing veins via cadaveric arteries is provided. This method is simple to operate, requiring only one artery puncture and one type of perfusion fluid. It is also less likely to cause dilation or deformity of the vein after perfusion, provides a clear anatomical view, and can be terminated when a mixture of perfusion fluid flows out of the vein. The perfused artery and vein exhibit different colors, and the color difference is visually discernible, thus achieving the differential display of arteries and veins. Attached Figure Description

[0028] Appendix Figure 1 This is a schematic diagram illustrating the principle of the present invention's method for perfusing veins into cadaver specimens via artery based on ink. In the diagram, 1 represents: a mixture of a certain color ink and perfusion fluid (before color change); 2: artery; 3: capillary network; 4: vein; 5: a mixture of a certain color ink and perfusion fluid (after color change). The arrows in the diagram indicate the flow direction of the perfusion fluid during the perfusion process.

[0029] Appendix Figure 2 This is a differential colorimetric image of the artery and vein after perfusion of a pig carcass specimen, as shown in Example 13.

[0030] Appendix Figure 3 This is a differential colorimetric image of the artery and vein after perfusion of a pig carcass specimen, as shown in Example 14.

[0031] Figure 4 shows an arterial and venous photograph of the pig carcass specimen after perfusion, as shown in Comparative Example 1. Figure 4A This is a photo of the blood vessels before they were opened. Figure 4B It's a photo taken after the blood vessels were cut open.

[0032] Appendix Figure 5 This is a photograph of the artery and vein of a pig carcass specimen after perfusion, as shown in Comparative Example 2. Detailed Implementation

[0033] The present invention will be further described below with reference to specific embodiments. It should be understood that these embodiments are for illustrative purposes only and are not intended to limit the scope of the invention. Furthermore, it should be understood that after reading the description of this invention, those skilled in the art can make various alterations or modifications to the invention, and these equivalent forms also fall within the scope defined by the appended claims.

[0034] Existing techniques for perfusing veins in cadaver specimens either involve direct perfusion via multiple punctures or arterial puncture followed by sequential infusion of different colored perfusion solutions to create color differences between arteries and veins. Based on extensive anatomical practice, the inventors' project provides a one-step method for perfusing veins via cadaveric arteries. This method requires only one artery puncture and the infusion of a single perfusion solution. The color change produced as the perfusion solution flows from the artery into the vein results in distinct colors for the artery and vein after perfusion, with the color difference being visually discernible, thus achieving the differential visualization of arteries and veins. The inventor's research, based on long-term anatomical practice, revealed during cadaveric vascular perfusion that adding various commercially available printing inks to the basic perfusion solution and then perfusing the mixture (color A) through the arterial end resulted in a color B after the mixture flowed out through the venous end. Furthermore, dissection revealed that the arterial vessels exhibited a clearly visible color A, while the venous vessels showed a clearly visible color B. This indicates that a color change occurred during the perfusion process, specifically as the mixture passed through the capillary network or entered the vein. Therefore, by using printing ink as a coloring additive in the perfusion solution, perfusing the mixture through arterial puncture, requiring only one artery puncture and one type of perfusion solution, a visible color difference can be achieved between arteries and veins, enabling the differentiation between them. (Appendix) Figure 1 This is a schematic diagram of the implementation principle of the present invention. Arterial puncture is performed, and a mixture of a certain color of perfusion fluid and printing ink (color A) is perfused through the arterial puncture site. The mixture flows through the artery-capillary network-venous direction and flows out from the venous puncture site. Perfusion is stopped after the mixture of perfusion fluid and printing ink (color B) flows out from the venous end.

[0035] Based on the above unexpected discovery and principle, the present invention provides a method for perfusing veins through arteries of cadaver specimens using printing ink. The method includes the steps of perfusing the arteries of cadaver specimens with a mixture of basic perfusing solution and printing ink, and stopping the perfusing after the perfusing reagent flows out of the vein.

[0036] In some specific embodiments of the present invention, the selection of the perfusion artery is based on the principle that any artery connected to the capillary network can be used as the perfusion artery. Specifically, it can be a head and neck artery, such as the common carotid artery, internal carotid artery, external carotid artery, superficial temporal artery, or facial artery; or an upper limb artery, such as the subclavian artery, axillary artery, brachial artery, radial artery, or ulnar artery; or a trunk artery, such as the thoracic aorta, abdominal aorta, renal artery, celiac trunk / branch artery, or mesenteric artery. In some preferred embodiments of the present invention, the perfusion artery is preferably the femoral artery or carotid artery.

[0037] In some specific embodiments of the present invention, the cadaver specimen specifically refers to the cadaver specimen of a higher vertebral individual with constant temperature and closed blood circulation, including but not limited to human cadaver specimens, pig cadaver specimens, rat cadaver specimens, rabbit cadaver specimens, dog cadaver specimens, monkey cadaver specimens, cow cadaver specimens, sheep cadaver specimens, horse cadaver specimens, chicken cadaver specimens, or duck cadaver specimens.

[0038] In some specific embodiments of the present invention, the base perfusion fluid is, for example, a natural latex perfusion fluid or a gelatin perfusion fluid. It should be noted that the base perfusion fluid is mainly used as a placebo medium in the present invention, its function being to fill and moderately dilate the target blood vessel, so that the vascular structure can be clearly displayed during anatomical or experimental observation. Furthermore, the base perfusion fluid is not limited to the above types; other conventional perfusion fluids that can pass through capillary networks and are suitable for vascular perfusion can also be selected, provided that the color-changing effect of the printing ink is not affected. Therefore, combinations of other commonly used base perfusion fluids with printing ink and their applications are also within the scope of protection of the present invention.

[0039] Example 1: One-step automated arteriovenous differential colorimetric perfusion reagent (I)

[0040] The one-step arteriovenous automatic differential colorimetric perfusion reagent includes a basic perfusion solution and printing ink, with a volume ratio of printing ink:basal perfusion solution = 1:150. The basic perfusion solution refers to pure latex solution, and the printing ink refers to Deli 9873 atomic printing ink.

[0041] Example 2: One-step automated arteriovenous differential colorimetric perfusion reagent (II)

[0042] The one-step arteriovenous automatic differential colorimetric perfusion reagent includes a basic perfusion solution and printing ink, with a volume ratio of printing ink:basal perfusion solution = 1:200. The basic perfusion solution refers to pure latex solution, and the printing ink refers to Deli 9873 atomic printing ink.

[0043] Example 3: One-step automated arteriovenous differential colorimetric perfusion reagent (Part 3)

[0044] The one-step arteriovenous automatic differential colorimetric perfusion reagent includes a basic perfusion solution and printing ink, with a volume ratio of printing ink:basal perfusion solution = 1:250. The basic perfusion solution refers to pure latex solution, and the printing ink refers to Deli 9873 atomic printing ink.

[0045] Example 4: One-step automated arteriovenous differential colorimetric perfusion reagent (IV)

[0046] The one-step arteriovenous automatic differential colorimetric perfusion reagent includes a basic perfusion solution and printing ink, with a volume ratio of printing ink:basal perfusion solution = 1:300. The basic perfusion solution refers to pure latex solution, and the printing ink refers to Deli 9873 atomic printing ink.

[0047] Example 5: One-step automated arteriovenous differential colorimetric perfusion reagent (V)

[0048] The one-step arteriovenous automatic differential colorimetric perfusion reagent includes a basic perfusion solution and printing ink, with a volume ratio of printing ink:basal perfusion solution = 1:350. The basic perfusion solution refers to pure latex solution, and the printing ink refers to Deli 9873 atomic printing ink.

[0049] Example 6: One-step automated arteriovenous differential colorimetric perfusion reagent (VI)

[0050] The one-step arteriovenous automatic differential colorimetric perfusion reagent includes a basal perfusion solution and printing ink, with a volume ratio of printing ink:basal perfusion solution = 1:200. The basal perfusion solution refers to a gelatin aqueous solution with a concentration of 5-100 mg / ml, and the printing ink refers to Deli 9873 atomic printing ink. The fluid temperature should be controlled above 40 degrees Celsius during vascular perfusion.

[0051] Example 7: One-step automated arteriovenous differential colorimetric perfusion reagent (VII)

[0052] The one-step arteriovenous automatic differential colorimetric perfusion reagent includes a basic perfusion solution and printing ink, with a volume ratio of printing ink:basal perfusion solution = 1:200. The basic perfusion solution refers to pure latex, and the printing ink refers to Chenguang advanced atomic printing ink.

[0053] Example 8: One-step automated arteriovenous differential colorimetric perfusion reagent (VIII)

[0054] The one-step arteriovenous automatic differential colorimetric perfusion reagent includes a basal perfusion solution and printing ink, with a volume ratio of printing ink:basal perfusion solution = 1:200. The basal perfusion solution refers to a gelatin aqueous solution with a concentration of 5-100 mg / ml, and the printing ink refers to Chenguang advanced atomic printing ink. The fluid temperature should be controlled above 40 degrees Celsius during vascular perfusion.

[0055] Example 9: One-step automated arteriovenous differential colorimetric perfusion reagent (IX)

[0056] The one-step arteriovenous auto-differential colorimetric perfusion reagent includes a basic perfusion solution and printing ink, with a volume ratio of printing ink:basal perfusion solution = 1:200. The basic perfusion solution refers to pure latex, and the printing ink refers to Jinsheng atomic printing ink.

[0057] Example 10: One-step automated arteriovenous differential colorimetric perfusion reagent (X)

[0058] The one-step arteriovenous autologous differential colorimetric perfusion reagent includes a basal perfusion solution and printing ink, with a volume ratio of printing ink:basal perfusion solution = 1:200. The basal perfusion solution refers to a gelatin aqueous solution with a concentration of 5-100 mg / ml, and the printing ink refers to Jinsheng atomic printing ink. The fluid temperature should be controlled above 40 degrees Celsius during vascular perfusion.

[0059] Example 11: One-step automated arteriovenous differential colorimetric perfusion reagent (XI)

[0060] The one-step arteriovenous automatic differential colorimetric perfusion reagent includes a basic perfusion solution and printing ink, with a volume ratio of printing ink:basal perfusion solution = 1:200. The basic perfusion solution refers to pure latex, and the printing ink refers to AsiaInfo atomic printing ink.

[0061] Example 12 One-step automated arteriovenous differential colorimetric perfusion reagent (XII)

[0062] The one-step arteriovenous autologous differential colorimetric perfusion reagent includes a basal perfusion solution and printing ink, with a volume ratio of printing ink:basal perfusion solution = 1:200. The basal perfusion solution refers to a gelatin aqueous solution with a concentration of 5-100 mg / ml, and the printing ink refers to AsiaInfo atomic printing ink. The fluid temperature should be controlled above 40 degrees Celsius during vascular perfusion.

[0063] Example 13 Method for Arterial Perfusion of Veins from Cadaver Specimens Based on Printed Oil (I)

[0064] Taking a pig carcass as an example, carotid artery puncture and perfusion are performed using the following method: For a fresh pig carcass specimen (1-3 days post-mortem), the skin is incised, the carotid artery is located and pulled outwards, a V-shaped incision is made to bleed the carotid artery. A thin rubber tube with a needle is inserted proximal to the incision site. A reagent is infused into the tube; the reagent consists of a basic perfusion solution and printing ink, with a volume ratio of printing ink:perfusion solution = 1:200. The basic perfusion solution refers to pure latex, and the printing ink refers to Deli 9873 atomic printing ink (blue). Perfusion is stopped after the perfusion solution flows out from the venous end. The arteries and veins (superficial circumflex iliac artery and superficial circumflex iliac vein) in the lower abdominal wall are dissected, as follows: Figure 2 As shown, the arteries and veins (superficial circumflex iliac artery and superficial circumflex iliac vein) of the lower abdominal wall are not dilated or deformed. The arteries and veins are different colors, and the color difference is visible to the naked eye, thus realizing the differential display of arteries and veins.

[0065] Example 14: Method for Arterial Perfusion of Veins from Cadaver Specimens Based on Printed Oil (Part Two)

[0066] Taking a pig carcass as an example, femoral artery puncture and perfusion are performed using the following method: For a fresh pig carcass specimen (1-3 days post-mortem), the skin is incised, the femoral artery is located and pulled outwards, a V-shaped incision is made to bleed the area. A thin rubber tube with a needle is inserted proximal to the incision site. A reagent is infused into the tube; the reagent consists of a basic perfusion solution and printing ink, with a volume ratio of printing ink:perfusion solution = 1:150. The basic perfusion solution refers to pure latex, and the printing ink refers to Jinsheng atomic printing ink. Perfusion is stopped after the perfusion solution flows out from the venous end. The carotid artery and vein are dissected, as follows: Figure 3 As shown, the carotid arteries and veins are not dilated or deformed. The arteries and veins are different colors, and the color difference is visible to the naked eye, thus achieving the display of the differences between arteries and veins.

[0067] Example 15: Method for Arterial Perfusion of Veins from Cadaver Specimens Based on Printed Oil (Part 3)

[0068] Taking a pig carcass as an example, femoral artery puncture and perfusion are performed using the following method: For a fresh pig carcass specimen (1-3 days post-mortem), the skin is incised, the femoral artery is located and pulled outwards, a V-shaped incision is made to release blood. A thin rubber tube with a needle is inserted distal to the incision, and a reagent is infused into the tube. The reagent consists of a basic perfusion solution and printing ink, with a volume ratio of printing ink:perfusion solution = 1:250. The basic perfusion solution refers to pure latex, and the printing ink refers to Chenguang advanced atomic printing ink. Perfusion is stopped after the perfusion solution flows out from the venous end. Dissection of the arteries and veins in the thigh reveals no dilation or deformation. The arteries and veins exhibit different colors, and the color difference is visually discernible, achieving differential visualization of arteries and veins.

[0069] Example 16: Method for Arterial Perfusion of Veins from Cadaver Specimens Based on Printed Oil (Part Four)

[0070] Taking a pig carcass as an example, femoral artery puncture and perfusion are performed using the following method: For a fresh pig carcass specimen (1-3 days post-mortem), the skin is incised, the femoral artery is located and pulled outwards, a V-shaped incision is made to bleed the area. A thin rubber tube with a needle is inserted proximal to the incision site, and a reagent is infused into the tube. The reagent consists of a basic perfusion solution and printing ink, with a volume ratio of printing ink:perfusion solution = 1:200. The basic perfusion solution refers to pure latex, and the printing ink refers to AsiaInfo atomic printing ink. Perfusion is stopped after the perfusion solution flows out from the venous end. Dissection of the carotid artery and vein reveals no dilation or deformation. The arteries and veins exhibit different colors, and the color difference is visually discernible, achieving differential visualization of arteries and veins.

[0071] Comparative Example 1

[0072] For fresh (1-3 days post-mortem) pig carcass specimens, incise the skin, locate the carotid artery, and retract it outwards to make a V-shaped incision for bloodletting. Insert a thin rubber tube with a needle into the proximal end of the blood vessel incision and infuse the tube with a reagent consisting of a basic perfusion solution and printing ink, in a volume ratio of printing ink:perfusion solution = 1:400. The basic perfusion solution refers to pure latex, and the printing ink refers to Chenguang high-grade atomic printing ink. Stop perfusion after the perfusion solution flows out from the venous end. Dissect the artery and vein in the thigh. (See Figure 4) Figure 4A These are photos of the arteries and veins in the thigh (without dissection). Figure 4B It is a photo of the arteries and veins in the thigh (after the blood vessels have been dissected). As you can see, the veins are white, and there is no obvious color difference between the arteries and veins.

[0073] Comparative Example 2

[0074] Taking a pig carcass as an example, femoral artery puncture and perfusion are performed using the following method: For a fresh pig carcass specimen (1-3 days post-mortem), the skin is incised, the femoral artery is located and pulled outwards, a V-shaped incision is made to bleed the area. A thin rubber tube with a needle is inserted proximal to the incision site. A reagent is infused into the tube; the reagent consists of a basic perfusion solution and printing ink, with a volume ratio of printing ink:perfusion solution = 1:100. The basic perfusion solution refers to pure latex, and the printing ink refers to AsiaInfo atomic printing ink. Perfusion is stopped after the perfusion solution flows out from the venous end. The carotid artery and vein are dissected, as follows: Figure 5 As shown, the jugular veins do not show obvious discoloration, resulting in a lack of clear color difference between the arteries and veins.

[0075] The above description is only a preferred embodiment of the present invention. It should be noted that those skilled in the art can make several improvements and additions without departing from the method of the present invention, and these improvements and additions should also be considered within the scope of protection of the present invention.

Claims

1. A one-step arterial perfusion automated differential colorimetric perfusion reagent for arteriovenous infusion, characterized in that, The infusion reagent includes printing ink, and the ratio of the volume of the printing ink to the total volume of the infusion reagent is 1:(151~351).

2. The one-step arterial perfusion automated differential colorimetric perfusion reagent according to claim 1, characterized in that, The ratio of the ink volume to the total volume of the injection reagent is 1:(151~251).

3. The one-step arterial perfusion automated differential colorimetric perfusion reagent according to claim 1, characterized in that, The perfusion reagent also includes a basic perfusion solution that can pass through a capillary network.

4. The one-step arterial perfusion automated differential colorimetric perfusion reagent according to claim 1, characterized in that, The base perfusion fluid is latex, gelatin solution, or other base perfusion fluid that can pass through the capillary network and does not affect the ink color-changing effect.

5. The use of printing ink in the preparation of automated arteriovenous differential colorimetric perfusion reagent.

6. The use according to claim 5, characterized in that, The preparation refers to mixing printing ink with the base injection fluid.

7. The use according to claim 6, characterized in that, The base perfusion fluid is latex, gelatin solution, or other base perfusion fluid that can pass through the capillary network and does not affect the ink color-changing effect.

8. The use according to claim 7, characterized in that, The volume ratio of the printing ink to the basic injection fluid is 1:(150~350).

9. A method for arterial perfusion of venous tissue from cadaver specimens based on printing ink, characterized in that, The procedure includes the step of perfusing the arteries of a cadaver specimen using the perfusion reagent described in any one of claims 1-4.

10. The method according to claim 9, characterized in that, It also includes the step of stopping the infusion after the infusion reagent flows out of the vein.

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