Light sheet microscopy and methods for light sheet microscopy

Abstract

A light-sheet microscope includes an illumination system configured to generate a light sheet. The light-sheet microscope also includes an optical system having an objective lens and a detector element. The optical system is configured to illuminate an illumination plane in a sample space via the objective lens using the light sheet, the illumination plane being tilted relative to the optical axis of the objective lens. The optical system is further configured to generate an image of a detection plane in the sample space via the objective lens using the detector element, the detection plane being tilted relative to the optical axis of the objective lens. The objective lens has a correction element configured to be adjustable for correcting aberrations. The light-sheet microscope further includes: a first adjustment unit configured to adjust the position of the illumination plane relative to a sample disposed in the sample space; a second adjustment unit configured to adjust the position of the detection plane relative to the sample; and a controller configured to control the first adjustment unit and / or the second adjustment unit to align the illumination plane with the detection plane based on the current setting of the correction element.

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