An alkaloid compound, and an extraction method and application thereof
Alkaloid compounds were extracted from Evodia rutaecarpa fruit using acid extraction and alkali precipitation, ethyl acetate extraction, and multi-step liquid chromatography purification methods. This method solved the problem of the lack of active ingredients against colorectal tumors in existing technologies and achieved effective inhibition of colorectal cancer cells.
CN122356079APending Publication Date: 2026-07-10THE 967TH HOSPITAL OF THE CHINESE PEOPLES LIBERATION ARMY JOINT LOGISTICS SUPPORT FORCE
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Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2026-05-11
- Publication Date
- 2026-07-10
AI Technical Summary
Technical Problem
There is no existing record of extracting alkaloid compounds as active ingredients for anti-colorectal tumors from Evodia rutaecarpa.
Method used
Alkaloid compounds were extracted from Evodia rutaecarpa fruit using a combination of acid extraction and alkali precipitation, ethyl acetate extraction, crude separation by ODS medium-pressure column chromatography, separation by reversed-phase semi-preparative HPLC, and purification by cyano column HPLC.
Benefits of technology
The extracted alkaloid compounds have a significant inhibitory effect on the proliferation of colorectal cancer cell line (HCT-116) and can be used to prepare anti-colorectal cancer drugs.
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Abstract
The application discloses an alkaloid compound and an extraction method and application thereof, and belongs to the technical field of alkaloid compounds. After crushing, the Evodia fruit is subjected to acid extraction and alkali precipitation treatment, ethyl acetate extraction, water washing and drying in sequence to obtain Evodia total alkaloids; the Evodia total alkaloids are subjected to ODS medium-pressure column chromatography, reverse-phase semi-preparative HPLC separation, cyanogen column HPLC primary purification and cyanogen column HPLC secondary purification in sequence to obtain the alkaloid compound. The alkaloid compound provided by the application is prepared from traditional Chinese medicine Evodia, has an inhibiting effect on the proliferation of a colorectal cancer cell line (HCT-116), and can be applied to the preparation of an anti-colorectal cancer tumor drug.
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