Metaprodigiosin production strain and separation and purification process thereof

By using the specific high-yield strain Streptomyces citrus YS-4 and its combination for separation and purification, the problems of low yield and low purity in the production of meclofenoxuron were solved, achieving efficient and high-purity preparation of meclofenoxuron, which is suitable for the development of drugs against drug-resistant bacteria.

CN122128375APending Publication Date: 2026-06-02HENAN AGRICULTURAL UNIVERSITY
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Patent Information

Application Number
CN202610229776.3
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-02-26
Publication Date
2026-06-02

AI Technical Summary

Technical Problem

Existing technologies for producing erythromycin from intermediate-loop cyclophosphamide strains exhibit poor metabolic specificity, low extraction yield, and difficulty in separation and purification, making it challenging to obtain high-purity products.

Method used

The specific high-yielding strain *Streptomyces citrus* YS-4 was used, combined with a combined technical route of fermentation regulation, biphasic extraction, polarity screening and hydrophobic purification. This included ethyl acetate extraction of the fermentation supernatant, methanol extraction of the bacterial cells, normal-phase silica gel chromatography and Sephadex LH-20 gel column chromatography, and finally purification by C18 reversed high-performance liquid chromatography.

Benefits of technology

The method achieves high-abundance extraction and high-purity separation of cyclophosphamide erythrin, with a product yield superior to that of the single solvent method, a product purity of 98.6%, and maintains biological activity, making it suitable for the development of drugs against drug-resistant bacteria.

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Abstract

The invention relates to the technical field of biological medicine and microbial fermentation engineering, and discloses an m-prodigiosin production strain and a separation and purification process thereof. The separation and purification process comprises the following steps: carrying out solid-liquid separation on fermentation liquor, respectively extracting supernate by adopting ethyl acetate, soaking in methanol to extract thalli, merging and concentrating to obtain a crude extract; and sequentially carrying out silica gel column chromatography and Sephadex LH-20 gel column chromatography purification on the crude extract, and finally carrying out C18 reverse semi-preparative high performance liquid chromatography refining to obtain the m-prodigiosin. According to the present invention, the yield is improved by using the dual extraction strategy, the efficient separation of the target product and the structure homolog is achieved through the specific chromatographic condition, the incomplete extraction problem caused by the coexistence of the intracellular adsorption and the extracellular secretion of the target product is effectively solved, and the extraction yield of the final product is superior to the single solvent extraction method.
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