A method for preparing FeCuMOF nanozymes and Pb based on FeCuMOF nanozymes 2+ Detection methods

By preparing FeCuMOF nanozymes and using an 'ON-OFF-ON' colorimetric sensing mechanism, the problems of insufficient catalytic activity and selectivity of nanozymes were solved, achieving high sensitivity and specificity for the detection of lead ions, which is suitable for rapid detection in irrigation water.

CN122356494APending Publication Date: 2026-07-10HENAN UNIV OF SCI & TECH
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Patent Information

Application Number
CN202610349553.0
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-03-20
Publication Date
2026-07-10

AI Technical Summary

Technical Problem

Existing nanozymes have insufficient catalytic activity and selectivity in the detection of lead ions, which limits their application in rapid detection.

Method used

FeCuMOF nanozymes were synthesized using a FeCuMOF nanozyme preparation method, which involved the use of ferric chloride hexahydrate, copper nitrate, and terephthalic acid. Cysteine ​​(CA) was used as a recognition element to construct an 'ON-OFF-ON' type colorimetric sensing mechanism, combining high peroxidase-like activity and specific recognition.

Benefits of technology

It achieves high sensitivity, specificity and rapid detection of lead ions, is suitable for trace detection in irrigation water, and has good recovery rate and accuracy.

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Abstract

A method for preparing FeCuMOF nanozymes and a detection method based on FeCuMOF nanozymes are disclosed, relating to the field of nanozyme sensing and detection technology. Preparation method: S11, ferric chloride hexahydrate and copper nitrate are added to N,N-dimethylformamide and stirred until dissolved; S12, terephthalic acid is added and stirred; S13, anhydrous ethanol, ultrapure water, and triethylamine are added, stirred, and the precipitate is collected; S14, the precipitate is washed and dried to obtain FeCuMOF nanozymes. Detection method: S21, standard solutions of multiple concentrations are prepared; S22, standard reaction systems and blank control systems of different concentrations are constructed; S23, the absorbance values ​​of the standard reaction systems and blank control systems are detected; S24, a standard curve is constructed; S25, a sample detection system is constructed; S26, the absorbance value of the sample detection system is detected, and the concentration in the sample is determined according to the standard curve. This invention can solve the technical problems in the prior art where insufficient catalytic activity and selectivity of nanozymes limit their application in detection.
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