A guided-mode resonance slot optical system of a biochip detection device

Abstract

This invention discloses a guided-mode resonant slot optical path system for a biochip detection device. The system comprises a modular, modular body integrally formed using additive manufacturing of polymer materials. It includes an illumination slot subsystem, a vertical slot subsystem, and an imaging focusing slot subsystem. The illumination slot subsystem defines the horizontal propagation direction of the main illumination optical axis through a series of optical element mounting brackets fixed to the illumination slot plate. The vertical slot subsystem is vertically connected to the illumination slot subsystem and the imaging focusing slot subsystem via a vertical slot side plate, forming a periscope-like vertical deflection optical path structure. The imaging focusing slot subsystem defines the receiving direction of the imaging optical axis and the emission and reflection directions of the infrared focusing optical axis through a series of optical element mounting brackets fixed to the imaging focusing slot plate. All optical elements are defined by the geometric tolerances of the mounting brackets, and the subsystems are rigidly assembled. This invention achieves automatic alignment of the entire optical path.

Description

Technical Field

[0001] This invention belongs to the field of precision opto-mechatronics integration and additive manufacturing error compensation technology, specifically relating to a guided-mode resonant slot optical path system for a biochip detection device. This system utilizes additive manufacturing processes to achieve a modular body construction under heterogeneous load distribution, enabling high signal-to-noise ratio physical field detection in unsteady environments. Background Technology

[0002] In the field of precision photoelectric detection, near-field enhancement via a specific substrate is of great significance for capturing weak signals. However, such systems exhibit extremely high sensitivity to the spatial positioning accuracy of optical components and the coaxiality of the optical axis. Traditional equipment is usually built based on discrete optical rails or breadboards, which, while offering high precision, are bulky and difficult to maintain stability under complex operating conditions.

[0003] While additive manufacturing using polymer materials can achieve lightweighting, it faces significant technical bottlenecks. First, additive manufacturing materials exhibit low modulus and creep strain over time, making them prone to cantilever torsional moments when supporting heavy-load components such as imaging cameras and stimulated emission sources, leading to optical axis misalignment at the micrometer scale. Second, uneven thermal shrinkage during 3D printing causes geometric deformation of the device body, disrupting the conjugate relationships of precise optical paths. Furthermore, internal heat accumulation in a closed detection environment can induce fluctuations in the air refractive index, further interfering with the resolution of weak signals. Therefore, there is an urgent need to develop a compact functional slot system capable of automatic alignment using intrinsic geometric tolerances and effectively compensating for material physical defects. Summary of the Invention

[0004] To address the shortcomings of existing biochip detection methods, such as extreme sensitivity to environmental vibrations, complex optical path alignment relying on discrete precision guide rails, and the tendency of additive manufacturing materials to creep and misalign under heavy loads, this invention provides a guide mold resonance slot optical path system for a biochip detection device. This system aims to directly define the geometric accuracy of the entire optical path through an integrated, split-type body, eliminating the need for expensive optical breadboards or discrete cage rod systems, thus enabling a portable biochip detection platform with autofocus and high geometric stability.

[0005] To solve the above-mentioned technical problems, the technical solution adopted by the present invention is as follows: In a first aspect, the present invention provides a guiding mode resonance slot optical path system for a biochip detection device. The system includes a modular split body integrally formed by additive manufacturing process using polymer materials. The modular split body includes an illumination slot subsystem, a vertical slot subsystem, and an imaging focusing slot subsystem that are mechanically locked together. The internal structure includes an illumination optical path channel, an imaging optical path channel, and a focusing detection channel that are interconnected and form a closed cavity structure. The illumination slot subsystem includes an illumination slot plate, which defines the horizontal propagation direction of the main illumination optical axis through a series of optical element mounting brackets fixed to the illumination slot plate; the vertical slot subsystem includes a vertical slot side plate, which is vertically connected between the illumination slot subsystem and the imaging focusing slot subsystem to form a periscope-like vertical turning optical path structure; the imaging focusing slot subsystem includes an imaging focusing slot plate, which defines the receiving direction of the imaging optical axis and the emission direction and reflection return direction of the infrared focusing optical axis through a series of optical element mounting brackets fixed to the imaging focusing slot plate. The system is configured as follows: an illumination slot subsystem emits illumination light, which is converged onto the biochip via a vertical slot subsystem, exciting the guided mode resonance effect on the surface of the biochip to generate imaging light carrying sample information; an imaging focusing slot subsystem emits infrared detection laser light, which is irradiated onto the biochip via a microscope imaging objective in the vertical slot system, and generated as focus feedback light after reflection from the biochip; the imaging light and the focus feedback light are received and transmitted to the imaging focusing slot subsystem via the microscope imaging objective; the imaging focusing slot subsystem is equipped with a dichroic mirror to perform spectral separation of the received imaging light and focus feedback light, so that the imaging light is transmitted to the imaging end, while the focus feedback light is guided to the focus sensing end, so as to sense and compensate for the slight drift of the sample focal plane in real time; The spatial six-degree-of-freedom coordinates, centrality, and coaxiality of all optical elements in the system are defined by the geometric tolerances of the fixed seats inside the split-type body. The geometric self-alignment of the entire optical path system is achieved through rigid assembly between subsystems.

[0006] According to the above technical solution, the lighting slot subsystem is provided with a laser source fixing seat, a first collimating lens fixing seat, a polarizer fixing seat, a half-wave plate fixing seat, a first focusing lens fixing seat, a pinhole fixing seat and a second collimating lens fixing seat in sequence along the lighting optical path, and a first right-angle adapter fixing seat at the end, which is used to deflect the lighting light to the vertical direction so that it can enter the vertical slot subsystem.

[0007] According to the above technical solution, the vertical slot subsystem is provided with a double cemented lens holder, a collimating objective lens holder and an imaging objective lens holder in sequence along the optical path axis. The imaging objective lens holder is used to assemble the microscopic imaging objective lens, and the biochip is placed above the microscopic imaging objective lens. The illumination light is sequentially converged onto the biochip via a doublet lens holder and a collimating objective lens holder, generating imaging light carrying sample information; the infrared detection laser is irradiated onto the biochip via a microscopic imaging objective lens, and after being reflected by the biochip, it generates focusing feedback light; the imaging light and the focusing feedback light together enter the imaging focusing slot subsystem via the microscopic imaging objective lens.

[0008] According to the above technical solution, the imaging focusing slot subsystem is provided with a second right-angle adapter fixing seat, a dichroic mirror right-angle adapter fixing seat, a tube lens fixing seat, an imaging camera lens fixing seat, and an imaging camera fixing seat in sequence along the imaging optical path, and is provided with an infrared laser source fixing seat, an infrared laser focusing lens fixing seat, an infrared laser collimating lens fixing seat, a neutral density filter fixing seat, a prism fixing seat, a cylindrical lens fixing seat, a filter fixing seat, a focusing camera lens fixing seat, and a focusing camera fixing seat in sequence along the focusing detection optical axis; The second right-angle adapter is used to deflect the infrared detection laser to the vertical direction so that it can enter the vertical slot subsystem and to deflect the imaging light and focusing feedback light received by the microscope imaging objective from the vertical direction to the horizontal direction so that they can enter the imaging focusing slot subsystem; the dichroic mirror right-angle adapter is used to assemble the dichroic mirror. The imaging light sequentially passes through the second right-angle adapter mount, the dichroic mirror right-angle adapter mount, the tube lens mount, the imaging camera lens mount, and the imaging camera mount to form an image; The infrared detection laser is generated by the infrared laser source in the infrared laser source holder, and passes sequentially through the infrared laser focusing lens holder, the infrared laser collimating lens holder, the neutral density filter holder, the prism holder, the dichroic mirror right-angle adapter holder, and the second right-angle adapter holder into the vertical slot subsystem, and is then irradiated onto the biochip by the microscope imaging objective. The focusing feedback light is received by the microscope imaging objective and transmitted to the imaging focusing slot subsystem. Then, it passes sequentially through the second right-angle adapter, the dichroic mirror right-angle adapter, the prism mount, the cylindrical lens mount, the filter mount, the focusing camera lens mount, and the focusing camera mount. A linear interference pattern is formed on the surface of the detector chip in the focusing camera mount. By extracting the centroid displacement of the pattern, real-time detection and closed-loop compensation of nanometer-level defocusing are achieved.

[0009] Following the above technical solution, the system also includes a clamping device, which includes an integrally formed clamping device fixing base and a clamping device body, with the clamping device body located on the clamping device fixing base; The bottom of the clamping device mounting base is provided with a fixing groove hole, and the clamping device is rigidly connected to the single-unit split body through the fixing groove hole; the side wall of the clamping device body is provided with a clamping device insertion hole and a displacement adjustment rod. The displacement adjustment rod is located in the clamping device insertion hole and is configured to limit the axial position displacement of the biochip by mechanical locking.

[0010] According to the above technical solution, the first right-angle adapter fixing seat or the second right-angle adapter fixing seat is used to assemble the right-angle adapter, the right-angle adapter includes an integrally formed reflector fixing boss and a reflector deflection light hole, which is used to deflect the light path.

[0011] Following the above technical solution, the dichroic mirror right-angle adapter fixing base is used to assemble the dichroic mirror right-angle adapter. The dichroic mirror right-angle adapter includes an integrally formed dichroic mirror fixing boss, a dichroic mirror limiting groove arranged at a preset angle, and a dichroic mirror turning light hole penetrating the central area, which is used to install the dichroic mirror and realize the spectral separation of imaging light and focusing feedback light.

[0012] Following the above technical solution, the modular split fuselage also includes a support column, which is made of high modulus hard metal material and has a first stud section and a second stud section at both ends. The lighting card slot plate is provided with an upper slot hole for the support column corresponding to the first stud section of the support column, and the imaging focusing card slot plate is provided with a lower slot hole for the support column corresponding to the second stud section of the support column. The support column extends longitudinally through the slot holes, establishing a rigid load-bearing skeleton structure between the lighting card slot subsystem and the imaging focusing card slot subsystem.

[0013] Following the above technical solution, all optical element mounting bases in the modular split fuselage adopt an adaptive geometric alignment structure, with an interference fit or tight tolerance clearance fit between the inner wall and the outer circumferential surface of the optical element, and the micro elastic modulus of the additive manufacturing polymer material is used to anchor the edge of the optical element at multiple points. Both the laser source holder and the infrared laser source holder adopt an elastic ring seat structure with stress-relieving slits. By adjusting the fasteners to shrink the slits, the cylindrical laser module can be centrally and symmetrically clamped, eliminating the optical axis tilt deviation caused by uneven thermal shrinkage in additive manufacturing.

[0014] In a second aspect, the present invention provides a biochip detection device, including the guided mode resonance slot optical path system described in the first aspect.

[0015] In summary, compared with the prior art, the above-described technical solutions conceived by this invention can achieve the following beneficial effects: First, the system achieves automatic alignment of the entire optical path. Since the spatial coordinates, centerness, and coaxiality of all optical elements are defined by the geometric tolerances of the fixed base integrally generated during the printing process within the modular body, the traditional discrete optical cages or guide rails are eliminated, greatly simplifying the assembly process and improving optical axis accuracy. Second, it possesses extremely high geometric stability and creep resistance. The rigid load-bearing skeleton constructed through hard metal support columns effectively offsets the torque of heavily loaded components, ensuring long-term optical axis stability during biochip signal acquisition. Third, it achieves highly robust environmental adaptability. The adaptive geometric alignment structure and elastic ring base design compensate for manufacturing deviations in additive manufacturing; the closed-loop autofocus and closed optical path channel work together to significantly improve the signal-to-noise ratio of weak light signal detection while solving the problem of focal plane drift in non-laboratory environments. Attached Figure Description

[0016] Figure 1 This is an external structural diagram of a guided mode resonant slot optical path system according to an embodiment of the present invention; Figure 2 This is a schematic diagram of the optical path of a guided mode resonant slot optical path system according to an embodiment of the present invention; Figure 3 This is a structural diagram of the illumination slot subsystem of the guided mode resonance slot optical path system according to an embodiment of the present invention; Figure 4 This is a structural diagram of the vertical slot subsystem of the guided mode resonance slot optical path system according to an embodiment of the present invention; Figure 5 This is a structural diagram of the imaging focusing slot subsystem of the guided mode resonance slot optical path system according to an embodiment of the present invention; Figure 6 This is a structural diagram of the clamping device of the guided mode resonant slot optical path system according to an embodiment of the present invention; Figure 7 This is a structural diagram of the support column of a guided mode resonant slot optical path system according to an embodiment of the present invention; Figure 8 This is a structural diagram of a dichroic mirror right-angle adapter for a guided mode resonance slot optical path system according to an embodiment of the present invention; Figure 9 This is a structural diagram of a right-angle adapter for a guided mode resonance slot optical path system according to an embodiment of the present invention.

[0017] In the diagram, 1 is the illumination slot subsystem; 10 is the illumination slot plate; 11 is the laser source mounting base; 12 is the first collimating lens mounting base; 13 is the polarizer mounting base; 14 is the half-wave plate mounting base; 15 is the focusing lens mounting base; 16 is the pinhole mounting base; 17 is the second collimating lens mounting base; 18 is the first right-angle adapter mounting base; 181 is the second right-angle adapter mounting base; 182 is the prism optical path channel; and 183 is the dichroic mirror right-angle adapter. 19. Mounting base; 2. Vertical slot subsystem; 20. Vertical slot side plate; 21. Double cemented lens mounting base; 22. Collimating objective lens mounting base; 23. Imaging objective lens mounting base; 3. Imaging focusing slot subsystem; 30. Imaging focusing slot plate; 31. Focusing camera mounting base; 32. Focusing camera lens mounting base; 33. Filter mounting base; 34. Cylindrical lens mounting base; 35. Prism mounting base; 36. Neutral density filter mounting base; 37. Infrared laser collimating lens mounting base; 38. Infrared laser focusing lens mounting base; 39. Infrared laser source mounting base; 4. Clamping device; 40. Imaging focusing slot plate thread; 41. Tube lens mounting base; 42. Lower slot of supporting column; 43. Imaging camera lens mounting base; 44. Imaging camera mounting base; 45. Clamping device fixing countersunk hole; 46. Clamping device mounting base; 47. Clamping device 48. Insertion hole; 5. Clamping device body; 6. Support column; 7. First stud section of support column; 8. Second stud section of support column; 9. Imaging focusing card slot subsystem fixing hole; 10. Support column slot hole; 11. Dichroic mirror right angle adapter; 22. Dichroic mirror fixing boss; 33. Dichroic mirror limiting groove; 44. Dichroic mirror turning aperture; 55. Right angle adapter; 66. Reflector fixing boss; 77. Reflector turning aperture. Detailed Implementation

[0018] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative and not intended to limit the invention. All other embodiments obtained by those skilled in the art based on the embodiments provided by this invention without inventive effort are within the scope of protection of this invention.

[0019] Obviously, the accompanying drawings described below are merely some examples or embodiments of the present invention. Those skilled in the art can apply the present invention to other similar scenarios based on these drawings without any inventive effort. Furthermore, it is understood that although the efforts made in this development process may be complex and lengthy, for those skilled in the art related to the content disclosed in this invention, modifications to design, manufacturing, or production based on the technical content disclosed in this invention are merely conventional technical means and should not be construed as insufficient disclosure of the present invention.

[0020] In this invention, the reference to "embodiment" means that a specific feature, structure, or characteristic described in connection with an embodiment may be included in at least one embodiment of the invention. The appearance of this phrase in various places in the specification does not necessarily refer to the same embodiment, nor is it a mutually exclusive, independent, or alternative embodiment. It will be explicitly and implicitly understood by those skilled in the art that the embodiments described in this invention may be combined with other embodiments without conflict.

[0021] Unless otherwise defined, the technical or scientific terms used in this invention shall have the ordinary meaning understood by one of ordinary skill in the art to which this invention pertains. The terms "a," "an," "an," "the," and similar words used in this invention do not indicate quantity limitation and may indicate singular or plural. The terms "comprising," "including," "having," and any variations thereof used in this invention are intended to cover non-exclusive inclusion; for example, a process, method, system, product, or device that includes a series of steps or modules (units) is not limited to the listed steps or units, but may also include steps or units not listed, or may include other steps or units inherent to these processes, methods, products, or devices. The terms "connected," "linked," "coupled," and similar words used in this invention are not limited to physical or mechanical connections, but may include electrical connections, whether direct or indirect. "A plurality" used in this invention refers to two or more. "And / or" describes the relationship between related objects, indicating that three relationships may exist; for example, "A and / or B" can represent: A alone, A and B simultaneously, and B alone. The character " / " generally indicates that the preceding and following objects have an "or" relationship. The terms "first," "second," and "third" used in this invention are merely to distinguish similar objects and do not represent a specific ordering of the objects.

[0022] This invention provides a guided-mode resonant slot optical path system for a biochip detection device. This system utilizes a polymer substrate with micro-elastic modulus, employing additive manufacturing to monolithically form multi-level functional modules. It is composed of an illumination slot subsystem with a physical interference locking interface, a vertical slot subsystem, and an imaging focusing slot subsystem, assembled to construct a self-shielded folding optical path. The system cavity integrates an asymmetric wavefront reshaping path, a signal acquisition unit, and an axial displacement compensation feedback module based on the principle of non-orthogonal reflection. During operation, an energy field is guided by a preset optical axis to act on the characteristic response substrate. The acquisition unit extracts minute perturbations in the energy distribution, and the feedback module dynamically calibrates the axial degree-of-freedom deviation of the carrier. This invention utilizes the endogenous limiting groove ridges formed during additive manufacturing to directly define the six-degree-of-freedom spatial coordinates of the optical element through geometric tolerance constraints. Furthermore, it introduces heterogeneous materials to reinforce the framework and counteract the time-dependent deformation of the polymer substrate, achieving geometric self-alignment and long-term optical axis stability without manual intervention.

[0023] The guided-mode resonant slot optical path system of the biochip detection device of the present invention includes a modular, split-type body integrally formed using polymer materials through additive manufacturing. This modular, split-type body comprises an illumination slot subsystem 1, a vertical slot subsystem 2, and an imaging focusing slot subsystem 3, which are mechanically interlocked and can be spliced ​​together. Figure 1 As shown. The fuselage contains interconnected, enclosed cavity structures for illumination, imaging, and focusing / detection optical paths.

[0024] In terms of structural layout, the illumination slot subsystem 1 defines the horizontal propagation direction of the main illumination optical axis through multiple optical element mounting seats fixed on the illumination slot plate 10; the vertical slot subsystem 2 is vertically connected between the illumination slot subsystem 1 and the imaging focusing slot subsystem 3 through the vertical slot side plate 20, forming a periscope-like vertical turning optical path structure; the imaging focusing slot subsystem 3 defines the receiving direction of the imaging optical axis and the emission direction and reflection return direction of the infrared focusing optical axis through a series of optical element mounting seats fixed on the imaging focusing slot plate 30.

[0025] The system is configured to: utilize the energy field in the excitation channel within the illumination slot subsystem 1 to act on the characteristic response substrate (i.e., biochip), and capture physical field phase interference signals through the demodulation channel; simultaneously, utilize the closed-loop feedback control module integrated within the imaging focusing slot subsystem 3 to sense and dynamically compensate for the minute drift of the carrier focal plane in real time.

[0026] The system as a whole does not contain any discrete optical guides or breadboards. The spatial six-degree-of-freedom coordinates, centrality, and coaxiality of all functional components in the system are defined by the geometric tolerances of the fixed seats integrally generated during the printing process inside the modular body. The geometric self-alignment of the entire beam path is achieved through the rigid assembly between subsystems.

[0027] like Figure 3 As shown, the lighting slot subsystem 1 integrates a spatial coherent field control path. Along the energy propagation path, this path sequentially includes a laser source mounting base 11, a first collimating lens mounting base 12, a polarizer mounting base 13, a half-wave plate mounting base 14, a first focusing lens mounting base 15, a pinhole mounting base 16, and a second collimating lens mounting base 17 with multi-lens mounting space. The first collimating lens mounting base 12 houses an aspherical collimating lens with a focal length of 5.5 mm, used for wavefront straightening correction of the initial coherent beam. The polarizer mounting base 13 and the half-wave plate mounting base 14 are used to define the initial vector direction and coupling direction of the energy field, respectively. The first focusing lens holder 15 is equipped with a plano-convex focusing lens with a focal length of 30mm, which is used to focus the beam through the micro-aperture filter pre-installed inside the pinhole holder 16 to optimize the far-field energy distribution characteristics. The second collimating lens holder 17 is equipped with an achromatic cemented doublet lens group with focal lengths of 150mm and 100mm respectively, which is used to reshape the regulated field distribution into a large-aperture uniform coherent detection field. The end of the illumination slot subsystem 1 is provided with a first right-angle adapter holder 18, which uses the reflective element fixed inside it to deflect the horizontal beam to the vertical direction so that the illumination light enters the vertical slot subsystem 2.

[0028] like Figure 4 As shown, the vertical slot subsystem 2 includes a relay signal guiding cavity enclosed by a vertical slot side plate 20. Within this cavity, along the energy propagation axis, are a double-cemented lens holder 21, a collimating objective holder 22, and an imaging objective holder 23 located at the terminal. The imaging objective holder 23 is used to assemble a component with high-resolution wavefront focusing characteristics, namely a high numerical aperture microscope imaging objective. The characteristic response substrate (i.e., a biochip) is placed above the microscope imaging objective. This microscope imaging objective is configured to both act as a converging end for the excitation field energy acting on the characteristic response substrate and as a capturing end for the response signal, collecting coherent physical field fluctuation data from the chip surface.

[0029] like Figure 5As shown, the imaging focusing slot subsystem 3 integrates an infrared autofocus feedback module based on the principle of total internal reflection. This subsystem, along the focusing detection optical axis, sequentially includes an infrared laser source mount 39, an infrared laser focusing lens mount 38, an infrared laser collimating lens mount 37, a neutral density filter mount 36, a prism mount 35, a cylindrical lens mount 34, a filter mount 33, a focusing camera lens mount 32, and a focusing camera mount 31. The infrared laser collimating lens mount 37 houses a collimating lens with a focal length of 25mm, and the infrared laser focusing lens mount 38 houses a collimating lens with a focal length of 50mm. The focusing lens, together with the infrared detection laser, collimates and expands the beam. The neutral density filter holder 36 is used to adjust the incident energy flux intensity of the detection light. The cylindrical lens holder 34 is equipped with a plano-convex cylindrical lens with a focal length of 250mm. This cylindrical lens is configured to focus the circular infrared beam reflected from the bottom surface of the sample in one dimension, so that it forms a linear interference pattern on the surface of the detector chip fixed on the focusing camera holder 31. The centroid displacement of this linear pattern in the pixel coordinate system is extracted in real time by the algorithm, thereby accurately calculating the nanometer-level defocus of the sample in the direction perpendicular to the optical axis and driving the actuator to perform closed-loop compensation. The imaging focusing slot subsystem 3 is also provided with a second right-angle adapter mounting base 181, a dichroic mirror right-angle adapter mounting base 183, a tube lens mounting base 41, an imaging camera lens mounting base 43, and an imaging camera mounting base 44 in sequence along the imaging optical path; a long-focal-length achromatic tube lens with a focal length coefficient of 250mm is pressed into the tube lens mounting base 41. This component is used to receive the parallel response beam from the dichroic mirror right-angle adapter 8, and to efficiently map the weak perturbation component carrying sample information onto the sensing plane of the photoelectric sensing unit inside the imaging camera mounting base 44 by utilizing its long focal length characteristic of 250mm.

[0030] The system is equipped with an axis deflection mechanism to determine the geometric orientation. This mechanism specifically includes a dichroic mirror right-angle adapter 8 and a right-angle adapter 9. For example... Figure 8 and Figure 9 As shown, the dichroic mirror right-angle adapter 8 is assembled on the dichroic mirror right-angle adapter fixing base 183, which has an integrally formed dichroic mirror fixing boss 81, a dichroic mirror limiting groove 82 arranged at a preset angle, and a dichroic mirror turning light hole 83 penetrating the central area; the right-angle adapter 9 is assembled on the first right-angle adapter fixing base 18 and the second right-angle adapter fixing base 181, which has a reflector fixing boss 91 and a reflector turning light hole 92; the functional element set inside the dichroic mirror right-angle adapter fixing base 183 is configured to separate the main detection beam and the focus feedback beam in the frequency domain, that is, to separate the imaging light and the focus feedback light, so that the visible energy field carrying spatial characteristics is transmitted to the output end, while the feedback field of a specific band is reflected to the axial deviation sensing module.

[0031] Specifically, such as Figure 2 As shown by the dotted line, the illumination light emitted by the illumination slot subsystem 1 is sequentially shaped by the laser source fixing seat 11, the first collimating lens fixing seat 12, the polarizer fixing seat 13, the half-wave plate fixing seat 14, the first focusing lens fixing seat 15, the pinhole fixing seat 16, and the second collimating lens fixing seat 17. Then, it is turned to the vertical direction by the right-angle adapter 9 in the first right-angle adapter fixing seat 18 and enters the vertical slot subsystem 2. After sequentially passing through the double cemented lens fixing seat 21 and the collimating objective lens fixing seat 22, it is converged and projected onto the biochip, stimulating the guided mode resonance effect on the surface of the biochip. The imaging light carrying sample information generated by the guided mode resonance effect is received by the microscope imaging objective in the imaging objective mounting 23 and then enters the vertical slot subsystem 2. It is transmitted to the right-angle adapter 9 in the second right-angle adapter mounting 181, and turns to the horizontal direction to enter the imaging focusing slot subsystem 3. It then passes through the dichroic mirror right-angle adapter mounting 183, the tube lens mounting 41, the imaging camera lens mounting 43, and the imaging camera mounting 44 in sequence, and is imaged on the detector in the imaging camera mounting.

[0032] like Figure 2 As shown by the long dashed line, the infrared laser source of the infrared laser source holder 39 in the imaging focusing slot subsystem 3 emits an infrared detection laser, which passes sequentially through the infrared laser focusing lens holder 38, the infrared laser collimating lens holder 37, the neutral density filter holder 36, and the prism holder 35. After passing through the dichroic mirror right-angle adapter 8 in the dichroic mirror right-angle adapter holder 183 and the right-angle adapter 9 in the second right-angle adapter holder 181, it is turned to the vertical direction and enters the vertical slot subsystem 2, where it is irradiated onto the biochip by the microscope imaging objective. The focusing feedback light reflected by the biochip is received by the microscopic imaging objective in the imaging objective mount 23 and returned to the imaging focusing slot subsystem 3. It is then turned to a horizontal direction by the right-angle adapter 9 in the second right-angle adapter mount 181, and then enters the prism optical path channel 182 through the dichroic mirror right-angle adapter mount 183. After that, it passes through the prism mount 35, the cylindrical lens mount 34, the filter mount 33, the focusing camera lens mount 32, and the focusing camera mount 31 in sequence, forming a linear interference pattern on the surface of the detector chip in the focusing camera mount. This pattern is used to extract the centroid displacement to achieve real-time detection and closed-loop compensation of nanometer-level defocus.

[0033] Each subsystem's card slot plate is provided with fixing holes for fixation. For example, the imaging focusing card slot plate 30 of the imaging focusing card slot subsystem 3 is provided with an imaging focusing card slot subsystem fixing hole 6, which has an imaging focusing card slot plate thread 40 inside, so that a set screw can be screwed in for fixation.

[0034] like Figure 1 and Figure 6As shown, the system also includes a clamping device 4 for back-field data acquisition and signal demodulation. The clamping device 4 is rigidly connected to the main body of the machine body through an integrally formed clamping device fixing seat 46 and a clamping device fixing slot hole 45. The side wall of the clamping device body 48 on the clamping device fixing seat 46 is provided with a clamping device insertion hole 47 and a displacement adjustment rod that communicate with its internal cavity. The displacement adjustment rod is configured to limit the axial position of the characteristic response substrate by mechanical locking, thereby ensuring that the spatial mapping conjugate relationship of the acquisition system does not drift due to environmental disturbances during high-sensitivity detection.

[0035] like Figure 7 As shown, the mechanical integrity and spatial geometric stability of this modular fuselage are maintained by a set of support columns 5. The support columns 5 are made of high-modulus hard metal material, and their geometric configuration includes a first stud section 51 and a second stud section 52. The lighting slot subsystem 1 and the imaging focusing slot subsystem 3 are vertically stacked and positioned by the thread meshing force. The lighting slot plate 10 is provided with a corresponding upper slot 19 of the support column, and a matching lower slot 42 of the support column is provided at the base end of the fuselage. The support column 5 extends longitudinally through the above-mentioned support column slot 7 and establishes a rigid force-bearing skeleton structure between multiple slot systems. This structural design can directly transmit the cantilever torsional torque generated by the heavy load components to the central axis of the support column 5, thereby avoiding the creep strain of the additive manufacturing fuselage material over time and ensuring the geometric determinism in the characteristic signal acquisition process.

[0036] All optical element mounting bases of this modular, split-type body employ an adaptive geometric alignment structure. The inner wall of the mounting base forms a preset interference fit or tight tolerance clearance fit with the corresponding outer circumferential surface of the optical element. The micro-elastic modulus of the additive manufacturing material is used to anchor the lens edge at multiple points, thereby replacing the traditional pressure ring locking structure. At the same time, both the laser source mounting base 11 and the infrared laser source mounting base 39 adopt an elastic annular seat structure with stress-relieving slits. By adjusting the fasteners on the outside of the body to shrink the slits, a centralized and symmetrical clamping of the cylindrical laser module is achieved. This eliminates the optical axis tilt deviation caused by uneven thermal shrinkage during 3D printing, ensuring the highly robust operation of the Kohler lighting system and autofocus system within a very small volume space.

[0037] The system utilizes a precision illumination shaping optical path within the illumination slot subsystem 1 to excite the guided mode resonance effect on the surface of the biochip. The vertical slot subsystem 2 includes a vertical light information transmission channel. An imaging objective mount 23, housing a high numerical aperture microscope imaging objective, serves both as a converging end for the illumination light projected onto the chip and as a scattering signal acquisition end for collecting interference information. Internally, a dichroic mirror right-angle adapter 8 separates the imaging light and focusing light spectrally, allowing visible light carrying image information to be transmitted to the imaging end, while reflecting the infrared focusing feedback light to the imaging focusing module. The imaging focusing slot subsystem 3 integrates a feedback module that uses a cylindrical lens to uniaxially focus the infrared beam reflected from the sample's bottom surface, forming a specific pattern on the detector surface. An algorithm extracts the centroid displacement in real time to compensate for minor drift of the sample's focal plane. Furthermore, the system includes a clamping device 4 rigidly connected via an integrated mounting base, using a displacement adjustment rod to limit the manual locking stroke, ensuring that the conjugate relationship remains unchanged due to environmental vibrations.

[0038] To maintain spatial geometric stability, the system employs a support column 5 made of high-modulus hard metal material, which runs longitudinally through multiple slot systems to establish a rigid load-bearing skeleton structure. This directly transmits the cantilever torsional moment generated by heavy-load components to the central axis of the column, thereby avoiding creep strain in the additive manufacturing material. All optical component mounts adopt an adaptive geometric alignment structure, utilizing the material's micro-elastic modulus to anchor the lenses at multiple points. The laser source mount uses an elastic ring-shaped structure with stress-relieving slits, eliminating optical axis tilt deviations caused by thermal shrinkage during 3D printing by adjusting the fasteners.

[0039] Furthermore, this invention also provides a biochip detection device, including the aforementioned guided-mode resonant slot optical path system. This system utilizes polymer materials and additive manufacturing processes to achieve a modular, modular construction of the body, thereby directly converting the spatial positioning accuracy of the precision optical system into the geometric properties of the body. The modular, modular body consists of an illumination slot subsystem 1, a vertical slot subsystem 2, and an imaging focusing slot subsystem 3, which are mechanically interlocked and can be connected to each other. Internally, it has interconnected illumination optical path channels, imaging optical path channels, and focusing detection channels with a closed cavity structure.

[0040] In terms of mechanical skeleton construction, the lighting slot subsystem 1 defines the propagation vector of the main energy axis through multiple functional fixing seats fixed on the lighting slot plate 10. This system abandons the traditional discrete cage rod, replacing it with a precision limiting groove ridge integrally generated during the printing process. The spatial coordinates, centerness, and coaxiality of all optical elements are defined by the geometric tolerances of the fixing seats. Utilizing the micro-elastic modulus of the polymer substrate, a preset interference fit is formed between the inner wall of the fixing seat and the element. Multi-point force anchoring replaces the traditional metal pressure ring locking. This adaptive geometric alignment structure eliminates the tilting deviation caused by manual assembly.

[0041] Specifically, the lighting slot subsystem 1 integrates a precision coherent field control path. This path, along the beam propagation path, sequentially includes a laser source mount 11, a first collimating lens mount 12, a polarizer mount 13, a half-wave plate mount 14, a first focusing lens mount 15, a pinhole mount 16, and a second collimating lens mount 17. The beam is first corrected for its wavefront by an aspherical collimating lens with a focal length of 5.5 mm installed inside the first collimating lens mount 12. Subsequently, the polarizer mount 13 and the half-wave plate mount 14 cooperate to define the initial vector state of the energy field. To optimize the far-field distribution, the system utilizes a plano-convex focusing lens with a focal length of 30 mm installed inside the first focusing lens mount 15 to converge the beam and pass it through a micrometer-level spatial filter inside the pinhole mount 16. The adjusted field distribution is reshaped into a highly flat parallel detection field by pressing achromatic doublet lens groups with focal lengths of 150mm and 100mm respectively into the second collimating lens holder 17.

[0042] To achieve long-term stable control under unsteady loads, the imaging focusing slot subsystem 3 integrates a real-time axial displacement calibration module based on the principle of non-orthogonal reflection. This module includes, sequentially along the axial direction, components such as an infrared laser source mount 39, an infrared laser focusing lens mount 38, and an infrared laser collimating lens mount 37. The detection energy source is emitted from the mount 39, and wavefront shaping is achieved through a collimating lens with a focal length of 25mm and a focusing lens with a focal length of 50mm.

[0043] The reflected return signal undergoes asymmetric axial mapping through a plano-convex cylindrical lens with a focal length of 250 mm inside the cylindrical lens mount 34. This configuration allows the beam to form a one-dimensional geometric pattern on the sensing plane of the focusing camera mount 31. When the sample undergoes axial displacement, the geometric centroid of the pattern shifts. The system uses closed-loop feedback compensation to ensure that the detection system maintains geometric determinism under environmental temperature drift or material creep disturbances.

[0044] To further resist stress misalignment caused by heavy loads, the system employs a rigid reinforced load-bearing frame. The split-type fuselage is maintained by a set of support columns 5, which are made of high-modulus hard metal material. Vertical stacking positioning is established through the meshing force of the first stud section 51 and the second stud section 52 of the support columns. This design directly transmits the cantilever torsional torque generated by heavy loads such as imaging cameras to the central axis of the columns, effectively preventing creep deformation of the polymer fuselage over time. In addition, the laser source fixing seat 11 adopts an elastic annular seat structure with stress-relieving slits, eliminating optical axis tilt deviation caused by 3D printing cooling shrinkage.

[0045] In summary, the first system achieves automatic alignment of the entire optical path. Since the spatial coordinates, centerness, and coaxiality of all optical elements are defined by the geometric tolerances of the fixed base integrally generated during the printing process, the traditional discrete optical cages or guide rails are eliminated, greatly simplifying the assembly process and improving optical axis accuracy. Secondly, it possesses extremely high geometric stability and creep resistance. The rigid load-bearing skeleton constructed through hard metal support columns effectively offsets the torque of heavily loaded components, ensuring long-term optical axis stability during biochip signal acquisition. Thirdly, it achieves highly robust environmental adaptability. The adaptive geometric alignment structure and elastic ring base design compensate for manufacturing deviations in additive manufacturing; the closed-loop autofocus system and the closed optical path channel work together to significantly improve the signal-to-noise ratio of weak light signal detection while solving the problem of focal plane drift in non-laboratory environments.

[0046] It should be noted that, depending on the implementation needs, the various steps / components described in this invention can be broken down into more steps / components, or two or more steps / components or parts of the operation of steps / components can be combined into new steps / components to achieve the purpose of this invention.

[0047] Those skilled in the art will readily understand that the above are merely preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of the present invention should be included within the scope of protection of the present invention.

Claims

1. A guided-mode resonant slot optical path system for a biochip detection device, characterized in that, The system includes a modular body integrally formed by additive manufacturing of polymer materials. The modular body includes an illumination slot subsystem, a vertical slot subsystem, and an imaging focusing slot subsystem that are mechanically locked together. It has an interconnected and enclosed cavity structure for the illumination optical path channel, the imaging optical path channel, and the focusing detection channel. The illumination slot subsystem includes an illumination slot plate, which defines the horizontal propagation direction of the main illumination optical axis through a series of optical element mounting brackets fixed to the illumination slot plate; the vertical slot subsystem includes a vertical slot side plate, which is vertically connected between the illumination slot subsystem and the imaging focusing slot subsystem to form a periscope-like vertical turning optical path structure; the imaging focusing slot subsystem includes an imaging focusing slot plate, which defines the receiving direction of the imaging optical axis and the emission direction and reflection return direction of the infrared focusing optical axis through a series of optical element mounting brackets fixed to the imaging focusing slot plate. The system is configured as follows: an illumination slot subsystem emits illumination light, which is converged onto the biochip via a vertical slot subsystem, exciting the guided mode resonance effect on the surface of the biochip to generate imaging light carrying sample information; an imaging focusing slot subsystem emits infrared detection laser light, which is irradiated onto the biochip via a microscope imaging objective in the vertical slot system, and generated as focus feedback light after reflection from the biochip; the imaging light and the focus feedback light are received and transmitted to the imaging focusing slot subsystem via the microscope imaging objective; the imaging focusing slot subsystem is equipped with a dichroic mirror to perform spectral separation of the received imaging light and focus feedback light, so that the imaging light is transmitted to the imaging end, while the focus feedback light is guided to the focus sensing end, so as to sense and compensate for the slight drift of the sample focal plane in real time; The spatial six-degree-of-freedom coordinates, centrality, and coaxiality of all optical elements in the system are defined by the geometric tolerances of the fixed seats inside the split-type body. The geometric self-alignment of the entire optical path system is achieved through rigid assembly between subsystems.

2. The guided-mode resonant slot optical path system of the biochip detection device according to claim 1, characterized in that, The illumination slot subsystem is provided with a laser source fixing seat, a first collimating lens fixing seat, a polarizer fixing seat, a half-wave plate fixing seat, a first focusing lens fixing seat, a pinhole fixing seat, and a second collimating lens fixing seat in sequence along the illumination optical path, and a first right-angle adapter fixing seat at the end, which is used to deflect the illumination light to the vertical direction so that it can enter the vertical slot subsystem.

3. The guided-mode resonant slot optical path system of the biochip detection device according to claim 1, characterized in that, The vertical slot subsystem is provided with a double cemented lens holder, a collimating objective lens holder, and an imaging objective lens holder in sequence along the optical path axis. The imaging objective lens holder is used to assemble the microscopic imaging objective lens, and the biochip is placed above the microscopic imaging objective lens. The illumination light is sequentially converged onto the biochip via a doublet lens holder and a collimating objective lens holder, generating imaging light carrying sample information; the infrared detection laser is irradiated onto the biochip via a microscopic imaging objective lens, and after being reflected by the biochip, it generates focusing feedback light; the imaging light and the focusing feedback light together enter the imaging focusing slot subsystem via the microscopic imaging objective lens.

4. The guided-mode resonant slot optical path system of the biochip detection device according to claim 1, characterized in that, The imaging focusing slot subsystem is provided with a second right-angle adapter fixing seat, a dichroic mirror right-angle adapter fixing seat, a tube lens fixing seat, an imaging camera lens fixing seat, and an imaging camera fixing seat in sequence along the imaging optical path. In addition, along the focusing detection optical axis, it is provided with an infrared laser source fixing seat, an infrared laser focusing lens fixing seat, an infrared laser collimating lens fixing seat, a neutral density filter fixing seat, a prism fixing seat, a cylindrical lens fixing seat, a filter fixing seat, a focusing camera lens fixing seat, and a focusing camera fixing seat in sequence. The second right-angle adapter is used to deflect the infrared detection laser to the vertical direction so that it can enter the vertical slot subsystem and to deflect the imaging light and focusing feedback light received by the microscope imaging objective from the vertical direction to the horizontal direction so that they can enter the imaging focusing slot subsystem; the dichroic mirror right-angle adapter is used to assemble the dichroic mirror. The imaging light sequentially passes through the second right-angle adapter mount, the dichroic mirror right-angle adapter mount, the tube lens mount, the imaging camera lens mount, and the imaging camera mount to form an image; The infrared detection laser is generated by the infrared laser source in the infrared laser source holder, and passes sequentially through the infrared laser focusing lens holder, the infrared laser collimating lens holder, the neutral density filter holder, the prism holder, the dichroic mirror right-angle adapter holder, and the second right-angle adapter holder into the vertical slot subsystem, and is then irradiated onto the biochip by the microscope imaging objective. The focusing feedback light is received by the microscope imaging objective and transmitted to the imaging focusing slot subsystem. Then, it passes sequentially through the second right-angle adapter, the dichroic mirror right-angle adapter, the prism mount, the cylindrical lens mount, the filter mount, the focusing camera lens mount, and the focusing camera mount. A linear interference pattern is formed on the surface of the detector chip in the focusing camera mount. By extracting the centroid displacement of the pattern, real-time detection and closed-loop compensation of nanometer-level defocusing are achieved.

5. The guided-mode resonant slot optical path system of the biochip detection device according to claim 1, characterized in that, The system also includes a clamping device, which includes an integrally formed clamping device mounting base and a clamping device body, with the clamping device body located on the clamping device mounting base. The bottom of the clamping device mounting base is provided with a fixing groove hole, and the clamping device is rigidly connected to the single-unit split body through the fixing groove hole; the side wall of the clamping device body is provided with a clamping device insertion hole and a displacement adjustment rod. The displacement adjustment rod is located in the clamping device insertion hole and is configured to limit the axial position displacement of the biochip by mechanical locking.

6. The guided-mode resonant slot optical path system of the biochip detection device according to claim 2 or 4, characterized in that, The first or second right-angle adapter mounting base is used to assemble the right-angle adapter, which includes an integrally formed mirror fixing boss and a mirror deflection aperture for deflecting the optical path.

7. The guided-mode resonant slot optical path system of the biochip detection device according to claim 4, characterized in that, The dichroic mirror right-angle adapter mounting base is used to assemble the dichroic mirror right-angle adapter. The dichroic mirror right-angle adapter includes an integrally formed dichroic mirror fixing boss, a dichroic mirror limiting groove arranged at a preset angle, and a dichroic mirror turning light hole that passes through the central area. It is used to install the dichroic mirror and realize the spectral separation of the imaging light and the focusing feedback light.

8. The guided-mode resonant slot optical path system of the biochip detection device according to claim 1, characterized in that, The modular fuselage also includes a support column, which is made of high-modulus hard metal material and has a first stud section and a second stud section at both ends. The lighting card slot plate is provided with an upper slot hole for the support column corresponding to the first stud section of the support column, and the imaging focusing card slot plate is provided with a lower slot hole for the support column corresponding to the second stud section of the support column. The support column extends longitudinally through the slot holes, establishing a rigid load-bearing skeleton structure between the lighting card slot subsystem and the imaging focusing card slot subsystem.

9. The guided-mode resonant slot optical path system of the biochip detection device according to claim 2 or 4, characterized in that, All optical element mounting bases in the modular body adopt an adaptive geometric alignment structure. The inner wall of the mounting base forms an interference fit or a tight tolerance clearance fit with the outer circumferential surface of the optical element. The micro elastic modulus of the additive manufacturing polymer material is used to anchor the edge of the optical element at multiple points. Both the laser source holder and the infrared laser source holder adopt an elastic ring seat structure with stress-relieving slits. By adjusting the fasteners to shrink the slits, the cylindrical laser module is centrally and symmetrically clamped, eliminating the optical axis tilt deviation caused by uneven thermal shrinkage in additive manufacturing.

10. A biochip detection device, characterized in that, The optical path system including the guided mode resonant slot as described in any one of claims 1 to 9.

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