Systems and methods for autofocus
The imaging system addresses autofocus challenges by using a self-calibrating hardware system with a reference beam to determine the z-position of a reflective interface, ensuring fast and accurate focus adjustment for diverse samples, including archival FFPE samples, without the need for fiducial markers.
WO2026147822A1PCT designated stage Publication Date: 2026-07-0910X GENOMICS INC
Patent Information
- Authority / Receiving Office
- WO · WO
- Patent Type
- Applications
- Current Assignee / Owner
- 10X GENOMICS INC
- Filing Date
- 2025-12-24
- Publication Date
- 2026-07-09
Smart Images
Figure US2025061281_09072026_PF_FP_ABST
Abstract
The present disclosure relates to an imaging system including: an objective having an optical axis defining a z-direction; an autofocus illumination module arranged to project, via the objective, a reference beam onto a reflective interface positioned in the field of view of the objective; and an image sensor arranged to image, via the objective, a reflection of the reference beam from the reflective interface. The autofocus illumination module is arranged so that the value of a parameter derivable from an object profile of the reference beam at the objective focal plane is an inflection value in the context of values of the parameter derivable from object profiles of the reference beam at planes corresponding to z-positions either side of the objective focal plane.
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