Disulfide isoform modulation

By regulating the disulfide subtype of recombinant proteins through contact with reducing agents or oxidizing agents, the problem of inconsistent disulfide structure distribution in existing technologies is solved, thereby improving the bioactivity and therapeutic efficacy of the IgG2 subclass.

CN122374330APending Publication Date: 2026-07-10GENENTECH INC
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Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-12-10
Publication Date
2026-07-10

AI Technical Summary

Technical Problem

Existing technologies struggle to effectively regulate the distribution of disulfide subtypes in recombinant proteins, leading to inconsistent biological activities. In particular, the IgG2 subclass exhibits low affinity for the Fc receptor, impacting the efficacy of therapeutic antibodies.

Method used

The distribution of disulfide isoforms of recombinant proteins can be regulated by contacting them with reducing or oxidizing agents. Specific methods include using reducing agents to increase the proportion of IgG2-B isoforms during culture or using oxidizing agents to maintain the amount of existing isoforms.

Benefits of technology

This study optimized and improved the bioactivity and consistency of recombinant proteins, enhanced the affinity of IgG2 subclasses for Fc receptors, and improved the biotherapeutic effect of therapeutic antibodies.

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Abstract

This invention relates to a method for regulating the distribution of disulfide isoforms in recombinant proteins. The invention also relates to a method for stabilizing the distribution of disulfide isoforms by preventing disulfide bond rearrangements in recombinant proteins, a method for producing recombinant proteins having regulated disulfide isoforms, and a method for altering the distribution of disulfide isoforms by enhancing disulfide bond rearrangements in recombinant proteins. The invention further relates to the use of reducing agents for regulating the distribution of disulfide isoforms by enhancing disulfide bond rearrangements in recombinant proteins, and the use of oxidizing agents for stabilizing the distribution of disulfide isoforms by preventing disulfide bond rearrangements in recombinant proteins.
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