Magnetic field generating device for cell sorting and control method thereof

Abstract

The application discloses a magnetic field generating device for cell sorting and a control method thereof, relates to the technical field of cell sorting devices, and comprises a fixing frame and a fixing shell, wherein the fixing frame and the fixing shell are both mounted on a rack, a driving mechanism is mounted on the fixing frame, the magnetic sorting of cells is realized by cooperation or separation of a clutch mechanism and the fixing shell driven by the driving mechanism, the clutch mechanism is mounted on the driving mechanism, a protection mechanism is mounted on the clutch mechanism, the protection mechanism is protected by the magnetism of a first permanent magnet on the clutch mechanism, a positioning mechanism is further mounted on the fixing shell, and the positioning mechanism is used for the positioning of a magnetic separator and the position adjustment of the first permanent magnet on the clutch mechanism. The magnetic field generating device for cell sorting and the control method thereof are characterized in that the linkage design of the positioning mechanism and the clutch mechanism realizes the dynamic self-adaptive adjustment of the magnetic field gradient, and effectively solve the problems of fixed magnetic field gradient and poor adaptability of the existing device.

Description

Technical Field

[0001] This invention relates to the field of cell sorting and generation devices, specifically to a magnetic field generating device and its control method for cell sorting. Background Technology

[0002] Magnetic cell sorting (MACS) is a relatively efficient and simple cell sorting method developed in the 1970s. The equipment and operation are simple, it does not cause mechanical damage to cells, and the separated cells have a high recovery rate and cell activity. Compared with flow cytometry, it has higher cell activity and is the preferred method for cell sorting with potential application prospects.

[0003] Magnetic cell sorting is based on the specific binding of antigens and antibodies in immunology. Magnetic microparticles are used as carriers, coated with antibodies or affinity ligands to form immunomagnetic composite microparticles. When incubated with mixed cells, the antibodies on the surface of the magnetic microparticles react specifically with the antigenic determinants on the cell surface, thus labeling the cells with the magnetic composite microparticles. The MACS magnetic sorting process: MACS microbeads are magnetically labeled. Under an applied magnetic field, cells with antibodies attached to the magnetic beads remain in the field due to the magnetism of the beads. Cells that do not express the antigen are not magnetic because they cannot bind to the specific antibodies on the surface of the magnetic beads and therefore cannot remain in the magnetic field. This separates target cells from non-target cells, resulting in highly pure target cells.

[0004] The existing device still has some shortcomings in practical use:

[0005] 1. Insufficient flexibility in magnetic field gradient adjustment: The permanent magnets in existing magnetic field generators are mostly fixed in position, making it impossible to dynamically adjust the spacing between them according to the specifications of the magnetic separator (such as diameter). This results in an unreasonable distribution of the magnetic field gradient. For large-diameter magnetic separators, the magnetic field gradient coverage is insufficient, and the magnetic field strength at the edge region decays too quickly, resulting in an uneven distribution where the gradient is extremely strong in the center and too weak at the edge, reducing cell capture efficiency. For small-diameter magnetic separators, the magnetic field gradient is too gentle, making it difficult to efficiently capture small-diameter or weakly magnetic particles, and thus failing to meet the needs of different sorting scenarios.

[0006] 2. The permanent magnets lack protective performance. In the existing devices, the permanent magnets are mostly exposed when demagnetized. During long-term idleness or repeated start-stop processes, they are prone to demagnetization due to irreversible changes in the magnetic domain structure, which leads to a decrease in magnetic field strength and affects the long-term stable operation and service life of the device. At the same time, magnetic field leakage during demagnetization may also cause interference to the surrounding environment and operators, posing a safety hazard. Summary of the Invention

[0007] The purpose of this invention is to provide a magnetic field generating device and its control method for cell sorting, so as to solve the problems mentioned in the background art.

[0008] To achieve the above objectives, the present invention provides the following technical solution: a magnetic field generating device for cell sorting, comprising a fixed frame and a fixed shell, both of which are mounted on a frame. A driving mechanism is mounted on the fixed frame, which drives a clutch mechanism to engage or disengage with the fixed shell to achieve magnetic cell sorting. The clutch mechanism is mounted on the driving mechanism and has a protective mechanism mounted on it, which is protected by the magnetic properties of a first permanent magnet on the clutch mechanism. A positioning mechanism is also mounted on the fixed shell, which is used for positioning the magnetic separator and adjusting the position of the first permanent magnet on the clutch mechanism.

[0009] Preferably, the fixed shell has cavities on the left and right sides of its rear side, and U-shaped clamping plates are symmetrically fixed on the fixed shell. The U-shaped clamping plates contact the magnetic separator to achieve positioning. The cavities can provide basic protection for magnetization and demagnetization, thereby ensuring the normal operation of the device. The U-shaped clamping plates can also be used to position and install the magnetic separator.

[0010] Preferably, the magnetic separator is equipped with an inlet valve at the upper end and an outlet valve at the lower end, and the outlet valve, the inlet valve and the U-shaped card are nested together. This structure provides a basic guarantee for the magnetic sorting of cells.

[0011] Preferably, the positioning mechanism includes a clamping plate that contacts the magnetic separator for positioning, and a rotatable swing plate is installed on the front side of the clamping plate. A torsion spring is connected between the swing plate and the clamping plate. A top rod is also fixed on the clamping plate. The top rod is slidably connected to the fixed shell. The clamping plate can clamp and position the magnetic separator, ensuring its stability. The swing plate can guide the magnetic separator, ensuring its proper installation.

[0012] Preferably, a fixing plate is fixed on the top rod, and the fixing plate and the crossbar are slidably connected. The crossbar is symmetrically fixed on the fixing shell, and a first spring is also fixed between the fixing plate and the fixing shell. The sliding action between the fixing plate and the crossbar can ensure the stability of the movement of the fixing plate. The elastic action of the first spring can provide a basic force for the reset of the clamping plate and a force for the clamping plate to hold the magnetic separator, thus ensuring the stability of the magnetic separator installation.

[0013] Preferably, the drive mechanism includes a reducer fixed on a fixed frame, and the reducer is driven by a motor. The output end of the reducer is connected to a threaded rod, and the threaded rod is connected to the fixed frame by a bearing. The threaded rod and the movable block are connected by a thread, and the movable block and the guide rail are connected by a sliding connection. The guide rail is symmetrically fixed on the fixed frame. Through the above structure, the movable block can perform stable linear motion, thereby providing a basic force for the movement of the clutch mechanism and ensuring the normal operation of the device.

[0014] Preferably, the clutch mechanism includes a connecting plate fixed to the movable block, and a mounting plate is fixed on the connecting plate. A fixing rod is symmetrically fixed to the rear end face of the mounting plate, and a movable plate is slidably connected to the fixing rod. The movable plate and the mounting plate are slidably connected, and a second spring is fixed between the movable plate and the mounting plate. The movable plate is fixed to the mounting box. First permanent magnets are uniformly installed inside the mounting box, and the magnetic poles of the opposing surfaces of the first permanent magnets in the left and right mounting boxes are exactly opposite. Inclined blocks are symmetrically fixed to the mounting box, and the inclined blocks are slidably connected to the top rod. Through the engagement and disengagement of the first permanent magnets with the cavity, magnetization and demagnetization can be achieved, thereby ensuring the normal operation of cell magnetic sorting. Furthermore, the sliding action between the inclined blocks and the top rod provides a basic guarantee for adjusting the distance between the two first permanent magnets, so as to adapt to magnetic separators of different diameters for magnetic sorting.

[0015] Preferably, the protective mechanism includes a vertical rod fixed on a fixed frame, and a guide groove plate is fixed at the upper end of the vertical rod. Moving rods are symmetrically slidably connected to the guide groove plate, and the moving rods are fixed to the baffle. A second permanent magnet is fixed on the baffle, and the second permanent magnet and the first permanent magnet are magnetically attracted to each other. Through the magnetic attraction between the second permanent magnet and the first permanent magnet, a closed magnetic circuit can be formed when the first permanent magnet is not in use, effectively preventing the first permanent magnet from demagnetizing.

[0016] Preferably, a sliding rod is also fixed on the baffle, and the sliding rod and the sliding groove are slidably connected. The sliding groove is opened on the horizontal plate, and the horizontal plate is fixed on the mounting plate. Through the sliding action between the sliding rod and the sliding groove, the separation and adsorption of the second permanent magnet and the first permanent magnet can be controlled, thereby ensuring the normal operation of the device.

[0017] A control method for a cell sorting magnetic field generator, comprising the following steps:

[0018] Step 1: First, install the magnetic separator containing nano magnetic beads and cell solution with the U-shaped clamp. At this time, the clamp can provide clamping force and limit the magnetic separator, and automatically adjust the position of the top rod according to the diameter of the magnetic separator.

[0019] Step 2: The clutch mechanism is driven by the drive mechanism to move, so that the first permanent magnet can cooperate with the cavity to realize the fine sorting of cells in the magnetic separator. When the first permanent magnet cooperates with the cavity, the distance between the left and right sets of first permanent magnets can be automatically adjusted by the sliding action between the inclined block and the top rod, so as to automatically adapt to magnetic separators of different diameters for magnetic sorting operation.

[0020] Step 3: After magnetic sorting is completed, the residual liquid can be discharged by opening the liquid outlet valve. After discharge, close the liquid outlet valve so that the sorted cells are stored in the magnetic separator.

[0021] Step 4: Drive the first permanent magnet to separate from the cavity through the drive mechanism to achieve demagnetization. After the first permanent magnet is reset, the magnetic circuit is closed through the magnetic attraction between the first permanent magnet and the second permanent magnet, which effectively reduces the demagnetization phenomenon that occurs when the first permanent magnet is not used for a long time.

[0022] Compared with the prior art, the beneficial effects of the present invention are:

[0023] 1. This magnetic field generating device and its control method for cell sorting, by setting a U-shaped clamping plate and a positioning mechanism on the fixed shell, forms a dual positioning structure, effectively solving the problems of unstable positioning and offset of the magnetic separator in existing devices. The U-shaped clamping plate is nested with the inlet and outlet valves of the magnetic separator to achieve initial positioning of the magnetic separator. The clamping plate in the positioning mechanism, under the elastic action of the first spring, forms a stable clamping of the magnetic separator. At the same time, the sliding guide action of the swing plate facilitates the quick and accurate installation of the magnetic separator, ensuring the coaxiality and stability of the magnetic separator after installation, avoiding magnetic field deviation caused by magnetic separator offset, and improving cell sorting accuracy. Furthermore, the positioning mechanism can automatically adjust the clamping plate spacing according to the diameter of the magnetic separator, adapting to different specifications of magnetic separators without the need to replace positioning components, significantly improving the applicability and versatility of the device.

[0024] 2. The magnetic field generating device and its control method for cell sorting, wherein the push rod in the positioning mechanism and the inclined block in the clutch mechanism slide in cooperation, the push rod moves a different distance when the diameter of the magnetic separator is different, and then drives the mounting box and the first permanent magnet to move through the inclined block, thereby adjusting the distance between the left and right sets of the first permanent magnets. For large-diameter magnetic separators, the distance between the two sets of the first permanent magnets is increased, which can cover a wider sorting area with the effective gradient magnetic field range, avoid uneven magnetic field distribution, and ensure cell capture efficiency. For small-diameter magnetic separators, the distance between the two sets of the first permanent magnets is reduced, which generates a stronger and steeper magnetic field gradient in a limited space, which can efficiently capture small-diameter or weakly magnetic particles, meet the sorting needs of different specifications of magnetic separators and different cell types. At the same time, the opposite magnetic poles of the left and right sets of the first permanent magnets can form a uniform and stable gradient magnetic field, further improving the separation effect of target cells and non-target cells, and obtaining target cells with higher purity. This solves the problem of low sorting efficiency and insufficient purity caused by unreasonable magnetic field gradient in existing devices.

[0025] 3. The magnetic field generating device and its control method for cell sorting, through a protective mechanism, allows the second permanent magnet to automatically separate from the first permanent magnet during the magnetization process, thereby ensuring the normal progress of magnetization and thus ensuring the magnetic sorting of cells. During the demagnetization process, the sliding cooperation between the slide rod and the slide groove allows the second permanent magnet to be attracted to the first permanent magnet to form a closed magnetic circuit, thereby effectively preventing the magnetic field strength of the first permanent magnet from decaying due to long-term idleness, and thus effectively improving the service life of the device. Attached Figure Description

[0026] Figure 1 This is a frontal three-dimensional structural diagram of the overall composition of the device of the present invention;

[0027] Figure 2 This is a rear-view three-dimensional structural diagram of the overall composition of the device of the present invention;

[0028] Figure 3 This is a three-dimensional structural diagram of the fixing shell of the present invention, viewed from the front and in cross-section.

[0029] Figure 4 This is a top-view three-dimensional structural diagram of the drive mechanism and clutch mechanism of the present invention;

[0030] Figure 5 This is a frontal perspective three-dimensional structural diagram of the mounting box of the present invention;

[0031] Figure 6 This is a frontal three-dimensional structural diagram of the protective mechanism of the present invention.

[0032] In the diagram: 1. Fixed frame; 2. Fixed shell; 201. Cavity; 202. U-shaped clamping plate; 3. Magnetic separator; 301. Inlet valve; 302. Outlet valve; 4. Positioning mechanism; 401. Clamping plate; 402. Swinging plate; 403. Top rod; 404. Fixed plate; 405. Crossbar; 406. First spring; 5. Drive mechanism; 501. Reducer; 502. Motor; 503. Threaded rod; 504. Movable block; 50 5. Guide rail; 6. Clutch mechanism; 601. Connecting plate; 602. Mounting plate; 603. Fixed rod; 604. Movable plate; 605. Second spring; 606. Mounting box; 607. First permanent magnet; 608. Inclined block; 7. Protective mechanism; 701. Vertical rod; 702. Guide groove plate; 703. Moving rod; 704. Baffle; 705. Second permanent magnet; 706. Sliding rod; 707. Sliding groove; 708. Horizontal plate. Detailed Implementation

[0033] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0034] Please see Figures 1-6 The present invention provides a technical solution: a magnetic field generating device for cell sorting, comprising a fixed frame 1 and a fixed shell 2. The fixed frame 1 and the fixed shell 2 are both mounted on a frame. A drive mechanism 5 is mounted on the fixed frame 1. The drive mechanism 5 drives a clutch mechanism 6 to cooperate or separate from the fixed shell 2 to achieve the magnetic sorting of cells. The clutch mechanism 6 is mounted on the drive mechanism 5. A protective mechanism 7 is mounted on the clutch mechanism 6. The protective mechanism 7 uses the magnetic protection of the first permanent magnet 607 on the clutch mechanism 6. A positioning mechanism 4 is also mounted on the fixed shell 2. The positioning mechanism 4 is used for positioning the magnetic separator 3 and adjusting the position of the first permanent magnet 607 on the clutch mechanism 6.

[0035] The fixed shell 2 has cavities 201 on the left and right sides of its rear side, and U-shaped clamping plates 202 are symmetrically fixed on the fixed shell 2, and the U-shaped clamping plates 202 contact the magnetic separator 3 to achieve positioning; the upper end of the magnetic separator 3 is equipped with an inlet valve port 301, and the lower end of the magnetic separator 3 is equipped with an outlet valve port 302, and the outlet valve port 302, the inlet valve port 301, and the U-shaped clamping plates 202 are nested together; the positioning mechanism 4 includes a clamping plate 401 that contacts the magnetic separator 3 to achieve positioning, and the clamping plate 401... A rotatable swing plate 402 is installed on the front side, and a torsion spring is connected between the swing plate 402 and the clamping plate 401. At the same time, a top rod 403 is fixed on the clamping plate 401, and the top rod 403 is slidably connected to the fixed shell 2. A fixed plate 404 is fixed on the top rod 403, and the fixed plate 404 is slidably connected to the cross bar 405. The cross bar 405 is symmetrically fixed on the fixed shell 2, and a first spring 406 is fixed between the fixed plate 404 and the fixed shell 2.

[0036] When using this magnetic field generator for cell sorting, such as Figures 1-6 As shown, firstly, nano-magnetic beads and cell solution are injected into the magnetic separator 3 through the inlet valve 301. After the injection is completed, the inlet valve 301 is closed. Then, the outlet valve 302, the inlet valve 301, and the U-shaped clamping plate 202 are nested together to achieve the positioning and installation of the magnetic separator 3. During the installation of the magnetic separator 3, when the magnetic separator 3 contacts the swing plate 402, the sliding guide action between the magnetic separator 3 and the swing plate 402 allows the magnetic separator 3 to contact the clamping plate 401. At this time, the clamping plate 401 is moved by force, thereby driving the top rod 403 and the fixing plate 404 to move, cooperating with the fixing plate 404. The sliding guide effect between the 4 and the crossbar 405 can ensure the stability of the movement of the clamping plate 401. At this time, the first spring 406 is compressed by force. Through the elastic action of the first spring 406, it can provide clamping force for the clamping plate 401, thereby ensuring the clamping and fixing effect of the clamping plate 401 on the magnetic separator 3 and ensuring the stability of the installation of the magnetic separator 3. According to the above principle, the larger the diameter of the magnetic separator 3, the farther the top rod 403 moves, thereby making the horizontal distance between the top rod 403 and the inclined block 608 closer, thus providing a basic guarantee for the subsequent adjustment of the distance between the two sets of first permanent magnets 607 on the left and right sides.

[0037] The drive mechanism 5 includes a reducer 501 fixed on the fixed frame 1, and the reducer 501 is driven by a motor 502. The output end of the reducer 501 is connected to a threaded rod 503. The threaded rod 503 is connected to the fixed frame 1 by a bearing. The threaded rod 503 is threadedly connected to the movable block 504, and the movable block 504 is slidably connected to the guide rail 505. The guide rail 505 is symmetrically fixed on the fixed frame 1. The clutch mechanism 6 includes a connecting plate 601 fixed to the movable block 504, and a mounting plate 602 is fixed on the connecting plate 601. The rear end face of the mounting plate 602 is left and right. A fixed rod 603 is symmetrically fixed, and a movable plate 604 is slidably connected to the fixed rod 603. The movable plate 604 is slidably connected to the mounting plate 602, and a second spring 605 is fixed between the movable plate 604 and the mounting plate 602. The movable plate 604 is fixed to the mounting box 606. A first permanent magnet 607 is evenly installed inside the mounting box 606, and the magnetic poles of the opposite faces of the first permanent magnets 607 in the left and right mounting boxes 606 are exactly opposite. Inclined blocks 608 are symmetrically fixed on the mounting box 606, and the inclined blocks 608 are slidably connected to the top rod 403.

[0038] After the magnetic separator 3 is installed, as follows Figures 1-6 As shown, at this time, by controlling the motor 502 to drive the reducer 501 to run, the threaded rod 503 rotates in the forward direction. With the threaded connection between the threaded rod 503 and the movable block 504, the movable block 504 can be moved by force. With the sliding guidance between the movable block 504 and the guide rail 505, the stability of the movement of the movable block 504 can be guaranteed.

[0039] When the movable block 504 moves, it synchronously drives the connecting plate 601, mounting plate 602, mounting box 606, first permanent magnet 607, and inclined block 608 forward. When the mounting box 606 and the first permanent magnet 607 enter the cavity 201, and during the forward movement of the inclined block 608, when the inclined block 608 contacts and slides against the top rod 403, the inclined block 608 is subjected to force and moves, thus synchronously driving the mounting box 606 and the first permanent magnet 607 to move. Combined with the sliding guide effect between the movable plate 604 and the fixed rod 603, the stability of the movement of the mounting box 606 and the first permanent magnet 607 can be ensured, thereby adjusting the distance between the left and right sets of first permanent magnets 607. The magnetic separation process continues until the connecting plate 601 contacts the fixed shell 2 to achieve positioning, thereby realizing the magnetic effect. The magnetic field formed between the two sets of first permanent magnets 607 on the left and right sides acts on the magnetic separator 3, which can realize the magnetic separation of cells in the magnetic separator 3 (cells connected to the magnetic beads with antibodies are retained in the magnetic field due to the magnetism of the magnetic beads, while cells that do not express this antigen are not magnetic because they cannot bind to the specific antibodies on the surface of the magnetic beads and cannot be retained in the magnetic field, thus separating the target cells from the non-target cells and obtaining target cells with higher purity). After the magnetic separation is completed, the residual liquid can be discharged by opening the liquid outlet 302. After the residual liquid is discharged, the liquid outlet 302 is closed, so that the target cells are retained in the magnetic separator 3 for subsequent processing.

[0040] Based on the above principle, the larger the diameter of the magnetic separator 3, the greater the moving distance of the top rod 403 relative to the fixed shell 2. That is, when the connecting plate 601 contacts the fixed shell 2 to achieve positioning, the sliding action between the inclined block 608 and the top rod 403 makes the moving distance between the mounting box 606 and the first permanent magnet 607 greater, that is, the greater the distance between the two sets of first permanent magnets 607. By increasing the spacing between the two sets of first permanent magnets 607, the effective gradient magnetic field range can cover a wider sorting area, avoiding the uneven situation where the central gradient is extremely strong and the edge is too weak, thus ensuring the cell capture efficiency. Conversely, the smaller the diameter of the magnetic separator 3, the smaller the distance between the two sets of first permanent magnets 607, thereby generating a stronger and steeper magnetic field gradient in a limited space, which can efficiently capture small-diameter or weakly magnetic particles and better meet the magnetic sorting needs of cells.

[0041] The protective mechanism 7 includes a vertical rod 701 fixed on the fixed frame 1, and a guide groove plate 702 fixed at the upper end of the vertical rod 701. A movable rod 703 is symmetrically slidably connected to the guide groove plate 702. The movable rod 703 is fixed to the baffle 704. A second permanent magnet 705 is fixed on the baffle 704, and the second permanent magnet 705 and the first permanent magnet 607 are magnetically attracted to each other. A sliding rod 706 is also fixed on the baffle 704, and the sliding rod 706 is slidably connected to the sliding groove 707. The sliding groove 707 is opened on the horizontal plate 708, and the horizontal plate 708 is fixed on the mounting plate 602.

[0042] As the mounting plate 602 moves forward, such as Figures 1-6 As shown, the synchronous movement of the horizontal plate 708 forward, combined with the sliding action between the inclined groove on the slide 707 and the slide rod 706, allows the two baffles 704 to move towards each other. The sliding action between the moving rod 703 and the guide plate 702 ensures the stability of the baffle 704's movement, thus separating the second permanent magnet 705 from the first permanent magnet 607. When the slide rod 706 slides to the straight groove on the slide 707, it ensures the normal forward movement of the first permanent magnet 607, thereby ensuring the normal magnetization process. When demagnetization is performed after cell sorting, based on the above principle, the motor 502 is activated to reduce... When the speed-driven mechanism 501 reverses the screw rod 503, the mounting plate 602 and the first permanent magnet 607 move backward and reset. At this time, when the sliding rod 706 slides from the straight groove on the sliding groove 707 to the inclined groove on the sliding groove 707, the mounting plate 602 separates from the cavity 201. Then, the mounting plate 602 and the first permanent magnet 607 continue to move backward and reset. With the sliding action between the sliding rod 706 and the inclined groove on the sliding groove 707, the second permanent magnet 705 can be attracted to the first permanent magnet 607 again, thus forming a closed magnetic circuit. This prevents the first permanent magnet 607 from demagnetizing due to prolonged lack of adaptation and ensures the service life of the device.

[0043] It should be noted that, in this document, the terms “comprising,” “including,” or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0044] This article uses specific examples to illustrate the principles and implementation methods of the present invention. The above examples are only for the purpose of helping to understand the method and core ideas of the present invention. The above descriptions are only preferred embodiments of the present invention. It should be noted that due to the limitations of textual expression, while there are objectively infinite specific structures, those skilled in the art can make several improvements, modifications, or changes without departing from the principles of the present invention, and can also combine the above technical features in an appropriate manner. These improvements, modifications, changes, or combinations, or the direct application of the inventive concept and technical solution to other situations without modification, should all be considered within the scope of protection of the present invention.

Claims

1. A magnetic field generating device for cell sorting, comprising a frame (1) and a housing (2), wherein the frame (1) and the housing (2) are both mounted on a frame, characterized in that: The fixed frame (1) is equipped with a drive mechanism (5). The drive mechanism (5) drives the clutch mechanism (6) to cooperate or separate from the fixed shell (2) to achieve the magnetic sorting of cells. The clutch mechanism (6) is installed on the drive mechanism (5). The clutch mechanism (6) is equipped with a protective mechanism (7). The protective mechanism (7) uses the magnetic protection of the first permanent magnet (607) on the clutch mechanism (6). The fixed shell (2) is also equipped with a positioning mechanism (4). The positioning mechanism (4) is used for positioning the magnetic separator (3) and adjusting the position of the first permanent magnet (607) on the clutch mechanism (6).

2. The magnetic field generating device for cell sorting according to claim 1, characterized in that: The fixed shell (2) has cavities (201) on the left and right sides of the rear side, and U-shaped card plates (202) are symmetrically fixed on the fixed shell (2) and the U-shaped card plates (202) contact the magnetic separator (3) to achieve positioning.

3. The magnetic field generating device for cell sorting according to claim 2, characterized in that: The magnetic separator (3) is equipped with an inlet valve (301) at the upper end and an outlet valve (302) at the lower end. The outlet valve (302), the inlet valve (301) and the U-shaped card plate (202) are nested together.

4. A magnetic field generating device for cell sorting according to claim 3, characterized in that: The positioning mechanism (4) includes a clamping plate (401) that contacts the magnetic separator (3) to achieve positioning, and a rotatable swing plate (402) is installed on the front side of the clamping plate (401), and a torsion spring is connected between the swing plate (402) and the clamping plate (401). At the same time, a top rod (403) is fixed on the clamping plate (401), and the top rod (403) is slidably connected to the fixed shell (2).

5. A magnetic field generating device for cell sorting according to claim 4, characterized in that: A fixing plate (404) is fixed on the top rod (403), and the fixing plate (404) and the crossbar (405) are slidably connected. The crossbar (405) is symmetrically fixed on the fixing shell (2) at the top and bottom. At the same time, a first spring (406) is also fixed between the fixing plate (404) and the fixing shell (2).

6. A magnetic field generating device for cell sorting according to claim 5, characterized in that: The drive mechanism (5) includes a reducer (501) fixed on the fixed frame (1), and the reducer (501) is driven by a motor (502). The output end of the reducer (501) is connected to the threaded rod (503). The threaded rod (503) is connected to the fixed frame (1) by a bearing. The threaded rod (503) is threadedly connected to the movable block (504), and the movable block (504) is slidably connected to the guide rail (505). The guide rail (505) is symmetrically fixed on the fixed frame (1).

7. A magnetic field generating device for cell sorting according to claim 6, characterized in that: The clutch mechanism (6) includes a connecting plate (601) fixed to the movable block (504), and a mounting plate (602) is fixed on the connecting plate (601). Fixing rods (603) are symmetrically fixed on the rear end face of the mounting plate (602). A movable plate (604) is slidably connected to the fixing rod (603). The movable plate (604) and the mounting plate (602) are slidably connected. A second spring (605) is also fixed between the movable plate (604) and the mounting plate (602). The movable plate (604) and the mounting box (606) are fixed to each other. A first permanent magnet (607) is evenly installed in the mounting box (606). The magnetic poles of the opposite faces of the first permanent magnets (607) in the left and right mounting boxes (606) are exactly opposite. Inclined blocks (608) are symmetrically fixed on the mounting box (606). The inclined blocks (608) and the top rod (403) are slidably connected.

8. A magnetic field generating device for cell sorting according to claim 7, characterized in that: The protective mechanism (7) includes a vertical rod (701) fixed on a fixed frame (1), and a guide groove plate (702) is fixed at the upper end of the vertical rod (701). A movable rod (703) is symmetrically slidably connected on the guide groove plate (702). At the same time, the movable rod (703) is fixed to the baffle (704). A second permanent magnet (705) is fixed on the baffle (704), and the second permanent magnet (705) and the first permanent magnet (607) form a magnetic attraction connection.

9. A magnetic field generating device for cell sorting according to claim 8, characterized in that: The baffle (704) is also fixed with a slide rod (706), and the slide rod (706) and the slide groove (707) are slidably connected. The slide groove (707) is opened on the horizontal plate (708), and the horizontal plate (708) is fixed on the mounting plate (602).

10. A control method for a cell sorting magnetic field generating device, applied to the cell sorting magnetic field generating device as described in claim 9, characterized in that, The specific steps are as follows: Step 1: First, install the magnetic separator (3) containing nano magnetic beads and cell solution with the U-shaped clamp (202). At this time, the clamp (401) can provide clamping force and limit the magnetic separator (3), and automatically adjust the position of the top rod (403) according to the diameter of the magnetic separator (3). Step 2: Drive the clutch mechanism (6) through the drive mechanism (5) to move so that the first permanent magnet (607) can cooperate with the cavity (201) to realize the fine sorting of cells in the magnetic separator (3). When the first permanent magnet (607) cooperates with the cavity (201), the distance between the two sets of first permanent magnets (607) can be automatically adjusted through the sliding action between the inclined block (608) and the top rod (403) so as to automatically adapt to magnetic separators (3) of different diameters for magnetic sorting operation. Step 3: After magnetic sorting is completed, the residual liquid can be discharged by opening the liquid outlet valve (302). After the discharge is completed, the liquid outlet valve (302) is closed so that the sorted cells are stored in the magnetic separator (3). Step 4: Drive the first permanent magnet (607) to separate from the cavity (201) through the drive mechanism (5) to achieve demagnetization. After the first permanent magnet (607) is reset, the magnetic circuit is closed through the magnetic attraction between the first permanent magnet (607) and the second permanent magnet (705), which effectively reduces the demagnetization phenomenon that occurs when the first permanent magnet (607) is not used for a long time.

Images