A method for preparing a midbrain organoid and uses thereof, and a method for pretreating a midbrain organoid
By optimizing the preparation method and in vitro processing of midbrain organoids, the problems of early fragility and insufficient long-range projection in the preparation of midbrain organoids were solved, and the preparation of high-purity A9 subtype cells and effective therapeutic effects in Parkinson's disease models were achieved.
Patent Information
- Application Number
- CN202610417352.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2026-03-31
- Publication Date
- 2026-07-17
AI Technical Summary
Existing methods for preparing midbrain organoids suffer from early graft fragility, insufficient long-range projection, and issues with donor preparation consistency and safety, making it difficult to achieve effective reconstruction of the substantia nigra-striatal pathway and neuronal replenishment.
Midbrain organoids were prepared using specific compositions and processing methods, including inducing compositions, modeling compositions, and maturation compositions. The proportion of A9 subtype cells was increased by optimizing culture conditions, and in vitro treatment with TNF-α inhibitors was used to enhance transplantation activity.
It improved the ventral identity purity and A9 bias of midbrain organoids, reduced early transplant apoptosis and inflammation, promoted the quantitative recovery of the substantia nigra-striatum pathway, and significantly improved the behavioral performance of Parkinson's disease model mice.
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Abstract
Citation Information
Patent Citations
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