Dicotyledon parthenocarpic induction gene miRWP and application thereof

By ectopically expressing a specific RWP gene in dicotyledonous plants and using recombinant expression vectors and genetic transformation methods, haploid induction in dicotyledonous plants was achieved, solving the technical problem of parthenogenetic haploid induction in dicotyledonous plants and providing genetic resources and technical solutions for breeding.

CN122405710APending Publication Date: 2026-07-17HUAZHONG AGRI UNIV +1
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Patent Information

Application Number
CN202610702975.1
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-03-06
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

In dicotyledonous plants, existing technologies make it difficult to efficiently induce haploids through parthenogenesis, which limits their application in breeding.

Method used

This paper provides the parthenogenesis-inducing gene MiRWP for dicotyledonous plants and its preparation method. By ectopically expressing specific RWP genes in dicotyledonous plants, these genes are introduced using recombinant expression vectors and Agrobacterium-mediated genetic transformation or gene gun methods to achieve oocyte-specific expression and induce haploid production.

Benefits of technology

The successful induction of haploids in dicotyledonous plants fills the gap in haploid induction for parthenogenesis in dicotyledonous plants, providing abundant genetic resources and technical solutions for breeding and expanding the application scope of haploid breeding technology.

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Abstract

本发明公开了一种双子叶植物孤雌生殖诱导基因MiRWP及其应用,具体是双子叶植物孤雌生殖诱导基因MiRWP在培育双子叶植物单倍体或单倍体诱导系或提高单倍体诱导能力中的应用,本发明首次发现并验证了RWP基因家族在双子叶植物中具有孤雌生殖单倍体诱导功能,填补了双子叶植物孤雌生殖单倍体诱导基因的空白。本发明为拓宽单倍体育种技术在双子叶植物上应用及揭示孤雌生殖单倍体产生的生物学机理奠定了重要的基础。鉴于目前育种行业中单倍体育种技术利用的广泛性,本发明在生物育种领域具有广泛的应用空间和前景。
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