A fermentation method for increasing the content of vitamin b12

By adding 5-aminolevulinic acid and controlling the total sugar content during the vitamin B12 fermentation process, the fermentation conditions were optimized, solving the problems of long fermentation cycle and low production level. This resulted in a shorter fermentation cycle and improved potency, making it suitable for industrial production.

CN122405779APending Publication Date: 2026-07-17NINGXIA KINGVIT PHARMA
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Patent Information

Application Number
CN202510050628.0
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-01-13
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

Existing vitamin B12 fermentation processes suffer from long fermentation cycles and low production levels, and fail to improve the potency of fermentation products through effective regulation of metabolic pathways.

Method used

During fermentation, a specific precursor, 5-aminolevulinic acid, is added, and the total sugar content is controlled at 1.0-7.0 g/100 ml. Fermentation conditions such as temperature, pressure, air flow rate, and stirring speed are optimized to shorten the fermentation cycle and enhance the metabolic pathway of vitamin B12.

Benefits of technology

It effectively shortened the fermentation cycle by 5.95%, increased the fermentation potency by 9.74%, met the needs of industrial production, and reduced production costs.

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Abstract

The present application provides a kind of fermentation method for improving the content of vitamin B12, by controlling total sugar content to be 1.0-7.0g / 100ml in the fermentation process, under the condition that 5-amino levulinic acid is added in the fermentation process of producing vitamin B12 in the fermentation medium by using Pseudomonas denitrificans, the metabolic pathway of vitamin B12 product synthesis is greatly strengthened.The fermentation method of the present application shortens the fermentation period and effectively improves the fermentation titer in the fermentation process of producing vitamin B12, and has great industrial application prospect.
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