Anamorphic diaphragm structure and microscopic illumination device

Abstract

The application provides a special-shaped diaphragm structure and a microscopic illumination device, which comprises a first circular ring and a second circular ring; the outer ring side of the first circular ring is provided with the second circular ring, and the second circular ring and the first circular ring are coaxially arranged; a light transmission area between the second circular ring and the first circular ring forms an annular diaphragm; a light transmission area on the inner ring side of the first circular ring forms a hole-shaped diaphragm; the hole-shaped diaphragm is located on the inner ring side of the annular diaphragm and is coaxially arranged with the annular diaphragm. The application can greatly enhance the clarity of the low-contrast transparent sample boundary profile, has smaller illumination intensity loss compared with phase contrast illumination, has high energy utilization rate, and can basically meet the rapid imaging requirements such as high-speed scanning and automatic focusing of a full-automatic microscope.

Description

Technical Field

[0001] This invention relates to the field of microscopic illumination devices, and more specifically, to irregularly shaped aperture structures and microscopic illumination devices. Background Technology

[0002] Currently, the microscopic observation of low-contrast transparent samples, such as unstained algae, bacteria, and live cells, often uses phase-contrast illumination. The disadvantage of this method is that the field of view is low and the required exposure time is long, which makes it difficult to meet the rapid imaging requirements of fully automated microscopes, such as high-speed scanning and autofocus. Summary of the Invention

[0003] In view of the deficiencies in the prior art, the purpose of this invention is to provide an irregularly shaped aperture structure and a microscopic illumination device.

[0004] According to the present invention, an irregularly shaped aperture structure includes: a first ring and a second ring;

[0005] A second ring is provided on the outer ring side of the first ring, and the second ring and the first ring are arranged at the same center;

[0006] The light-transmitting area between the second ring and the first ring forms an annular aperture, and the light-transmitting area on the inner ring side of the first ring forms an aperture-shaped aperture. The aperture-shaped aperture is located on the inner ring side of the annular aperture and is co-centered with the annular aperture.

[0007] Preferably, the inner ring side of the second ring is connected to the outer ring side of the first ring through a plurality of thin rod-shaped connecting structures.

[0008] Preferably, the plurality of thin rod-shaped connecting structures divide the light-transmitting area between the second ring and the first ring into a plurality of fan-shaped areas of the same shape.

[0009] Preferably, the ratio of the light-transmitting area of ​​the aperture to that of the annular aperture is 1:100 to 10:1.

[0010] Preferably, a microscopic illumination device employs the aforementioned irregular aperture structure.

[0011] Preferably, it includes: an irregularly shaped aperture structure and a condenser lens module;

[0012] The irregularly shaped aperture structure is provided at the front end of the condenser lens module facing the sample direction.

[0013] Preferably, the outer side of the lens of the condenser module near the sample direction is provided with the aperture-shaped aperture and the annular aperture of the irregular aperture structure.

[0014] Preferably, a cylindrical housing is connected to the outer ring side of the second ring of the irregular aperture structure, and the cylindrical housing is fitted onto the end of the condenser lens module.

[0015] Compared with the prior art, the present invention has the following beneficial effects:

[0016] 1. This application can greatly enhance the clarity of the boundary contour of low-contrast transparent samples. Compared with phase-contrast illumination, its illumination intensity loss is small and its energy utilization rate is high, which can basically meet the rapid imaging needs of fully automatic microscopes such as high-speed scanning and autofocus.

[0017] 2. This invention only requires the addition of an irregularly shaped aperture structure to the traditional lighting system, without the need for major changes to optical components, and can be quickly modified for various microscopes. Attached Figure Description

[0018] Other features, objects, and advantages of the present invention will become more apparent from the following detailed description of non-limiting embodiments with reference to the accompanying drawings:

[0019] Figure 1 This is a schematic diagram of the irregularly shaped aperture structure;

[0020] Figure 2 Schematic diagram of an irregularly shaped aperture structure installed on a condenser lens module;

[0021] Figure 3 This is a schematic diagram of the irregularly shaped aperture structure.

[0022] As shown in the figure:

[0023] Detailed Implementation

[0024] The present invention will now be described in detail with reference to specific embodiments. These embodiments will help those skilled in the art to further understand the present invention, but do not limit the invention in any way. It should be noted that those skilled in the art can make several changes and improvements without departing from the concept of the present invention. These all fall within the protection scope of the present invention.

[0025] In this embodiment, an irregular aperture structure 1, consisting of a concentric aperture 11 and an annular aperture 12, is used on the microscope condenser module 2 to achieve complementary central and oblique illumination. The central illumination ensures the uniformity and intensity of the illumination, while the oblique illumination utilizes the changes in refraction and scattering of different media under transparent samples to enhance the contrast of the boundary contour of the transparent sample.

[0026] Specifically, such as Figure 1As shown, this embodiment includes: a first ring and a second ring; the second ring is disposed on the outer ring side of the first ring, and the second ring and the first ring are concentrically arranged; the inner ring side of the second ring is connected to the outer ring side of the first ring through multiple thin rod-shaped connecting structures. The multiple thin rod-shaped connecting structures divide the light-transmitting area between the second ring and the first ring into multiple fan-shaped areas of the same shape. The light-transmitting area between the second ring and the first ring forms an annular aperture 12, and the light-transmitting area on the inner ring side of the first ring forms an aperture 11, which is located on the inner ring side of the annular aperture 12 and is concentrically arranged with the annular aperture 12.

[0027] In one embodiment, the ratio of the light-transmitting area of ​​the aperture 11 to the annular aperture 12 is 1:100 to 10:1, which means that the contribution of the aperture 11 and the annular aperture 12 to the sample illumination intensity is between 1:20 and 20:1.

[0028] like Figure 2 As shown, a microscopic illumination device with the irregular aperture structure 1 of this embodiment is installed, including: the irregular aperture structure 1 and the condenser lens module 2; the irregular aperture structure 1 is provided at the front end of the condenser lens module 2 facing the sample direction. A cylindrical housing is connected to the outer ring side of the second ring of the irregular aperture structure 1, and the cylindrical housing is fitted onto the end of the condenser lens module 2.

[0029] In one implementation, such as Figure 3 As shown, the outer side of the lens of the condenser module 2 near the sample direction is provided with an aperture-shaped aperture 11 and an annular aperture 12 of the irregular aperture structure 1. That is, the irregular aperture structure 1 is placed between the condenser module 2 and the sample, and is close to the position of the last lens of the condenser module 2.

[0030] In one embodiment, the cylindrical housing of the irregular aperture structure 1 can be connected to the outer shell of one end of the condenser module 2 by thread, or it can be installed by interference fit, adhesive bonding or other methods.

[0031] In one embodiment, the cylindrical shell of the irregular aperture structure 1 has various diameters to accommodate condenser lens modules 2 of different sizes.

[0032] In the description of this application, it should be understood that the terms "upper", "lower", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this application.

[0033] Specific embodiments of the present invention have been described above. It should be understood that the present invention is not limited to the specific embodiments described above, and those skilled in the art can make various changes or modifications within the scope of the claims, which do not affect the essence of the present invention. Unless otherwise specified, the embodiments and features described in this application can be arbitrarily combined with each other.

Claims

1. An irregularly shaped aperture structure, characterized in that, include: First ring and second ring; A second ring is provided on the outer ring side of the first ring, and the second ring and the first ring are arranged at the same center; The light-transmitting area between the second ring and the first ring forms an annular aperture (12), and the light-transmitting area on the inner ring side of the first ring forms an aperture-shaped aperture (11). The aperture-shaped aperture (11) is located on the inner ring side of the annular aperture (12) and is co-centered with the annular aperture (12).

2. The irregular aperture structure according to claim 1, characterized in that: The inner ring side of the second ring is connected to the outer ring side of the first ring through multiple thin rod-shaped connecting structures.

3. The irregular aperture structure according to claim 1, characterized in that: The multiple thin rod-shaped connecting structures divide the light-transmitting area between the second ring and the first ring into multiple fan-shaped areas of the same shape.

4. The irregular aperture structure according to claim 1, characterized in that: The ratio of the area of ​​the light-transmitting region of the aperture (11) to that of the annular aperture (12) is 1:100 to 10:

1.

5. A microscopic illumination device, characterized in that: The irregular aperture structure described in any one of claims 1-4 is adopted.

6. The microscopic illumination device according to claim 5, characterized in that, include: Irregular aperture structure (1) and condenser lens module (2); The condenser lens module (2) has the irregular aperture structure (1) at its front end facing the sample direction.

7. The microscopic illumination device according to claim 6, characterized in that: The condenser lens module (2) has an aperture-shaped aperture (11) and an annular aperture (12) of the irregular aperture structure (1) on the outer side of the lens near the sample direction.

8. The microscopic illumination device according to claim 6, characterized in that: The outer ring of the second ring of the irregular aperture structure (1) is connected to a cylindrical shell, which is fitted onto the end of the condenser lens module (2).

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