Imaging method and system based on high-speed axial zoom technology

Abstract

The application discloses an imaging method and system based on high-speed axial zooming technology, and the method comprises the following steps: acquiring imaging requirement information of a sample to obtain a requirement signal; in the case that the requirement signal is imaging preview, starting a vibration exciter, driving a mirror to axially scan a sample on a sample bin, and adjusting a shutter speed of a camera according to a motion cycle of the vibration exciter and sampling and imaging the sample; in the case that the requirement signal is rapid imaging, stopping the vibration exciter, adjusting the shutter speed of the camera according to a motion cycle of the sample bin and sampling and imaging the sample. By setting two speed imaging modes, the requirements of axial resolution, sampling rate, sampling preview and imaging time under different conditions can be met, and the imaging convenience and compatibility based on the high-speed axial zooming technology are improved.

Description

Technical Field

[0001] This invention relates to the field of light sheet microscope imaging technology based on high-speed axial zoom technology, and in particular to an imaging method and system based on high-speed axial zoom technology. Background Technology

[0002] In light-sheet microscopy, the generation and scanning of the light sheet are the core elements of light-sheet microscopy imaging technology. The imaging system illuminates the sample under examination through the light sheet, targeting and exciting fluorescence signals in the sample layer corresponding to the focal plane of the imaging objective lens. Imaging is performed in an orthogonal direction, effectively avoiding the influence of defocused background on image quality, and featuring high imaging contrast and natural optical tomography. By moving the relative position between the transparent sample and the static light sheet, tissues within the sample located at the focal plane are excited to emit fluorescence signals, which are captured by the imaging arm in an orthogonal direction to the focal plane. The system moves the sample axially, simultaneously capturing two-dimensional fluorescence images of different layers of the sample using a digital camera. A three-dimensional data point set is established during sample movement, and with the aid of graphics processing software, a three-dimensional visualization and reconstruction of the sample is achieved.

[0003] In existing technologies, only scientific research systems possess similar imaging modes. Axial illumination technology improves resolution by scanning the illumination direction of the light sheet and synchronizing the light sheet waist position with the camera's rolling shutter exposure area, thereby reducing the impact of out-of-focus information on imaging. Its core lies in the performance parameters of the zoom system. In recent years, widely adopted zoom methods include piezoelectric ceramic motors, voice-coil motors, and electro-liquid lenses (ETL), but each method has its own drawbacks in terms of cost, stability, optical aberrations caused by zooming, and zoom travel. Summary of the Invention

[0004] To address the aforementioned problems, the present invention aims to provide an imaging method, system, and storage medium based on high-speed axial zoom technology. By setting two speed imaging modes, it meets the requirements for axial resolution, sampling rate, sampling preview, and imaging time under different conditions, thereby improving the convenience and compatibility of imaging based on high-speed axial zoom technology.

[0005] The technical solution adopted by this invention to solve its problem is:

[0006] In a first aspect, embodiments of this application provide an imaging method based on high-speed axial zoom technology. The method includes: acquiring imaging requirement information of a sample to obtain a requirement signal; when the requirement signal is an imaging preview, turning on an exciter and driving a reflector to perform axial scanning of the sample on the sample chamber, with the camera adjusting the shutter speed in coordination with the motion cycle of the exciter and sampling the sample; when the requirement signal is a fast imaging signal, turning off the exciter, with the camera adjusting the shutter speed in coordination with the motion cycle of the sample chamber and sampling the sample.

[0007] Secondly, embodiments of this application provide a light sheet microscope imaging system based on high-speed axial zoom technology, comprising: an acquisition module for acquiring imaging requirement information of a sample to obtain a requirement signal; a first sampling module for activating an exciter and driving a mirror to perform axial scanning of the sample on the sample chamber when the requirement signal is an imaging preview, and a camera adjusting the shutter speed in coordination with the motion cycle of the exciter to sample and image the sample; and a second sampling module for deactivating the exciter when the requirement signal is a fast imaging signal, and the camera adjusting the shutter speed in coordination with the motion cycle of the sample chamber to sample and image the sample.

[0008] Thirdly, embodiments of this application provide an electronic device, including: a memory, a processor, and a computer program stored in the memory and executable on the processor. When the processor executes the computer program, it implements the imaging method based on high-speed axial zoom technology as described above.

[0009] Fourthly, embodiments of this application provide a computer-readable storage medium storing a computer program, which, when executed by a processor, implements the imaging method based on high-speed axial zoom technology as described above.

[0010] In this embodiment, by acquiring the imaging requirements of the sample, and based on these requirements, the exciter is activated to drive the reflector to perform axial scanning of the sample. The camera adjusts the shutter speed according to the exciter's motion cycle and samples the sample for imaging, providing specific location information, retrograde standard imaging, and a preview of the required signal. Alternatively, the exciter can be deactivated, and the camera adjusts the shutter speed according to the sample chamber's motion cycle and samples the sample for imaging, enabling rapid, high-definition imaging of large samples. These two imaging modes can meet the requirements for axial resolution, sampling rate, sampling preview, and imaging time under different conditions, improving the convenience and compatibility of imaging based on high-speed axial zoom technology.

[0011] Additional aspects and advantages of this application will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of this application. Attached Figure Description

[0012] Figure 1 This is a schematic flowchart of an imaging method based on high-speed axial zoom technology according to an embodiment of this application;

[0013] Figure 2 yes Figure 1 A schematic diagram illustrating the specific implementation process of another embodiment of step S1000;

[0014] Figure 3 yes Figure 1 A schematic diagram illustrating the specific implementation process of another embodiment of step S2000;

[0015] Figure 4 yes Figure 1 A schematic diagram illustrating the specific implementation process of another embodiment of step S3000;

[0016] Figure 5 This is a structural diagram of a light sheet microscope imaging system based on high-speed axial zoom technology according to an embodiment of this application;

[0017] Figure 6 yes Figure 5 Schematic diagram of the structure of the first sampling module and the second sampling module;

[0018] Figure 7 This is a schematic diagram of the structure of an electronic device provided in an embodiment of this application. Detailed Implementation

[0019] To make the objectives, technical solutions, and advantages of this application clearer, the following detailed description is provided in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative and not intended to limit the scope of this application.

[0020] It should be understood that in the description of the embodiments of this application, the use of terms such as "first" and "second" is only for the purpose of distinguishing technical features and should not be construed as indicating or implying relative importance, or implicitly indicating the number of technical features indicated, or implicitly indicating the order of the technical features indicated. "At least one" refers to one or more, and "more" refers to two or more. "And / or" describes the relationship between related objects, indicating that three relationships can exist. For example, A and / or B can represent the existence of A alone, the simultaneous existence of A and B, or the existence of B alone. A and B can be singular or plural. The character " / " generally indicates that the preceding and following related objects are in an "or" relationship. "At least one of the following" and similar expressions refer to any combination of these items, including any combination of single or plural items. For example, at least one of a, b, and c can represent: a, b, c, a and b, a and c, b and c, or a and b and c, where a, b, and c can be single or multiple.

[0021] Furthermore, the technical features involved in the various embodiments of this application described below can be combined with each other as long as they do not conflict with each other.

[0022] The imaging method based on high-speed axial zoom technology disclosed in this application is based on light-sheet microscopy and a three-dimensional tissue imaging mode. By moving the relative position between the transparent sample and the static light sheet, the tissue located at the focal plane in the sample is excited to emit a fluorescence signal, which is captured by an imaging arm in the direction orthogonal to the focal plane. Then, by axially moving the sample, two-dimensional fluorescence images of different layers of the sample are simultaneously captured by a digital camera. A three-dimensional data point set is established during the sample movement, and with the help of graphics processing software, a three-dimensional visualization reconstruction of the sample is achieved.

[0023] The imaging method based on high-speed axial zoom technology disclosed in this application eliminates spherical and chromatic aberration caused by the system lenses during zooming by designing a symmetrical optical path structure, ensuring stable axial resolution over a wide field of view. A lower-cost, higher-performance exciter is used for the zoom mechanism. By conjugating the focal point of the optical sheet to a free-space position, a mirror is used to scan the optical sheet axially, causing the conjugate position of the optical sheet to change axially. In this case, the moving component of the axial zoom system is not the objective lens, but a mirror mounted on the exciter that exceeds the cross-sectional area of ​​the optical sheet. This effectively reduces the motion stress on the scanning equipment, improving the imaging speed of the imaging system and significantly increasing the travel distance of the zoom optical path. However, existing imaging methods based on high-speed axial zoom technology can only perform a single imaging acquisition method and cannot adjust the operating parameters between the camera, exciter, and sample chamber according to actual imaging needs to meet the requirements for axial resolution, sampling rate, sampling preview, and imaging time under different conditions, thus affecting the widespread application of imaging methods based on high-speed axial zoom technology.

[0024] Based on the above, this application provides an imaging method, system, and storage medium based on high-speed axial zoom technology. By acquiring the imaging requirements of the sample, and according to these requirements, an exciter is activated to drive a reflector to perform axial scanning of the sample. The camera adjusts the shutter speed in accordance with the exciter's motion cycle and samples the sample for imaging, providing specific location and retrograde standard imaging, as well as a preview of the required signal. Alternatively, the exciter can be turned off, and the camera adjusts the shutter speed in accordance with the sample chamber's motion cycle to sample the sample for imaging, enabling rapid high-definition imaging of large samples. These two imaging modes can meet the requirements for axial resolution, sampling rate, sampling preview, and imaging time under different conditions, improving the convenience and compatibility of imaging based on high-speed axial zoom technology.

[0025] Please see Figure 1 , Figure 1 The flowchart illustrates an imaging method based on high-speed axial zoom technology provided in an embodiment of this application. For example... Figure 1 As shown, the imaging method based on high-speed axial zoom technology in this application includes the following steps:

[0026] S1000 acquires the imaging requirement information of the sample and obtains the requirement signal.

[0027] It is understandable that high-speed axial zoom technology is used to image samples with large surface areas. These samples typically require rapid and high-resolution imaging without the need for image preview. Alternatively, it can be used to image samples with smaller imaging areas and a certain thickness. These samples generally do not have high requirements for speed and accuracy, but image preview is still necessary. Therefore, different imaging modes should be selected for samples of different sizes and with different imaging needs to meet the diverse imaging requirements of high-speed 3D sampling and online sample observation, thereby improving the imaging speed of samples.

[0028] Please see Figure 2 , Figure 2 A schematic diagram illustrating the specific implementation process of another embodiment of step S1000 described above is shown. For example... Figure 2 As shown, the imaging method based on high-speed axial zoom technology in this application includes the following steps:

[0029] S1100 acquires imaging requirements information for the sample, including the imaging area, scanning thickness, and imaging speed.

[0030] It is understandable that samples with different imaging areas, scanning thicknesses, and imaging speeds have different imaging requirements. Therefore, before performing imaging operations, it is necessary to obtain the imaging requirements of the samples so that the imaging method based on high-speed axial zoom technology can meet the requirements for axial resolution, sampling rate, sampling preview, and imaging time under different conditions.

[0031] It is understandable that imaging requirements such as the imaging area, scanning thickness, and imaging speed of the sample can be automatically acquired through the data acquisition system (DAQ) or manually entered through the client. The process and methods of acquiring the imaging area, scanning thickness, and imaging speed of the sample are existing technologies and will not be elaborated here.

[0032] S1200 obtains the required signal based on the imaging area, scanning thickness, and imaging speed.

[0033] Understandably, by collecting and analyzing the imaging area, scanning thickness, and imaging speed of the sample, it is possible to determine whether the sample needs imaging preview and then output a demand signal. In specific applications, if the imaging area of ​​the sample is less than a preset area threshold, the scanning thickness of the sample reaches a preset thickness threshold, and the required imaging speed of the sample is less than a preset speed threshold, it can be determined that the sample needs imaging preview; that is, the demand signal is imaging preview, and the exciter is turned on in imaging mode to facilitate the search for sample feature surfaces and structures and to perform sampling imaging. If the imaging area of ​​the sample is greater than a preset area threshold, the scanning thickness of the sample is less than a preset thickness threshold, and the required imaging speed of the sample is greater than a preset speed threshold, it can be determined that the sample does not need imaging preview; that is, the demand signal is fast imaging, and the exciter is turned off in imaging mode to improve the imaging speed of the sample.

[0034] It is understandable that the demand signal includes, but is not limited to, imaging preview and rapid imaging. In practical applications, it can also be defined according to specific imaging needs, so as to quickly and accurately obtain the demand signal based on the imaging demand information and select the corresponding imaging mode.

[0035] Understandably, in practical applications, users can directly input whether a sample needs to be previewed for imaging on the client side, based on their needs, so that imaging requirements can be set more flexibly and quickly, ensuring the compatibility of imaging methods based on high-speed axial zoom technology.

[0036] S1300: The sample is placed on the sample chamber, where the sample chamber and the exciter are located on opposite sides of the polarization cube. Objective lenses are provided between the sample chamber and the exciter and the polarization cube, and the objective lenses form symmetrical optical paths on both sides of the polarization cube.

[0037] Understandably, in order to ensure that the sample is in a position that is convenient for the camera to collect imaging data during the imaging process, the sample needs to be fixed on the sample chamber so that the sample can move with the sample chamber, making it convenient to scan and image different positions of the sample.

[0038] It is understandable that the positional relationship between the sample chamber, polarizing cube, objective lens, and exciter is as follows: Figure 6 As shown. The imaging method based on high-speed axial zoom technology in this application mainly consists of a symmetrical optical path composed of the objective lens between the sample chamber and the polarization cube, and the objective lens between the exciter and the polarization cube.

[0039] In practical applications, a lens group is also installed between the sample chamber and the polarization cube to correct spherical and chromatic aberrations generated during the movement of the reflector. The lens group is optimized for different dispersion values ​​and lens shapes at different wavelengths to achieve minimal chromatic aberration. Specifically, the lens group is a set of achromatic cemented doublet lenses with the same focal length.

[0040] When the demand signal is an imaging preview, the S2000 activates the exciter and drives the reflector to perform axial scanning of the sample in the sample chamber. The camera adjusts the shutter speed according to the movement cycle of the exciter and samples and images the sample.

[0041] Understandably, the laser beam emitted by the laser enters the polarization cube, and after passing through the polarization cube, most of the laser beam is reflected into the right side of the symmetrical optical path. After being focused by the high-power objective lens 2, the focal point falls in front of and behind the reflecting mirror. The reflecting mirror reflects the focused beam back into the symmetrical optical path, and the beam continues to travel from right to left. It passes sequentially through a lens group consisting of lens 1, lens 2, lens 3, and lens 4. The function of the lens group is to correct spherical aberration and chromatic aberration generated during the movement of the reflecting mirror. Similarly, it is focused onto the sample by the high-power objective lens 1. Since the position of objective lens 1, which determines the convergence position of the light plate, and the preceding optical path are completely symmetrical with the position of objective lens 2, which determines the position of the reflecting mirror, the focal points in front of and behind the reflecting mirror are also completely conjugate with the position of the sample. Therefore, moving the reflecting mirror along the symmetrical optical path causes the beam to form a real focus and a virtual focus in front of and behind the reflecting mirror, respectively. This is equivalent to changing the original focusing position of the beam in this optical path and moving the focusing position in front of and behind the reflecting mirror. Similarly, the position of the sample behind objective lens 2 also moves back and forth from its original position. At this point, simply adjusting the frequency and amplitude of the vibrator's forward and backward movement to match the camera's shutter movement direction with the vibrator's movement direction will enable axial zoom technology.

[0042] In practical applications, the standard sampling mode involves activating the exciter, using a reflector to perform axial scanning of the sample in the sample chamber, and adjusting the shutter speed in conjunction with the exciter's motion cycle to sample and image the sample. The imaging system described above has a maximum axial resolution of 610 nanometers, which can be adjusted online as needed to meet the tomographic requirements of different tissues in the sample.

[0043] Please see Figure 3 , Figure 3 A schematic diagram illustrating the specific implementation process of another embodiment of step S2000 described above is shown. For example... Figure 3 As shown, the imaging method based on high-speed axial zoom technology in this application includes the following steps:

[0044] S2100, adjust the shutter speed so that the camera's line scan rate matches the exciter's motion cycle.

[0045] Understandably, since the exciter is in the activated state, driving the reflector to move back and forth, it is necessary to control the camera's shutter movement direction to match the exciter's movement direction to perform image acquisition of the sample. At this time, the camera is excited by an external square wave signal, and the exciter's triangular wave signal originates from the DAQ. The camera's data acquisition frequency is consistent with the sample chamber's movement speed, and the signal originates from DAQ control. For example, one image is acquired for every 1 nanometer movement of the sample according to a preset area (X1, Y1, W1, H1).

[0046] The S2200 controls the camera's shutter speed through external modulation, ensuring that the camera's excitation signal period equals the sum of the exciter's motion period and the preset phase difference.

[0047] Understandably, to ensure the camera can perform imaging sampling in sync with the exciter's motion cycle, the camera's shutter speed is controlled via external modulation, synchronizing the camera's excitation signal period with the exciter's motion cycle. Specifically, the camera's excitation signal period equals the sum of the exciter's motion period and a preset phase difference, ensuring the camera's shutter operation is synchronized with the exciter and guaranteeing complete acquisition of sample imaging data. Controlling the camera's shutter speed via external modulation is existing technology and will not be elaborated upon here.

[0048] S2300 performs sampling and imaging of a preset area of ​​the sample based on the vibration cycle of the exciter.

[0049] Understandably, after the exciter is activated, the sample chamber displacement step size is limited by the full-frame preview interface, and the movement speed is limited by the zoom speed. Furthermore, the camera shutter scanning speed also needs to be adapted to the current zoom speed. Therefore, standard mode sampling based on high-speed axial zoom technology, with its full-frame preview capability, facilitates the identification of characteristic surfaces and structures of the sample for sampling and imaging.

[0050] When the required signal is rapid imaging, the S3000 turns off the exciter, and the camera adjusts the shutter speed according to the motion cycle of the sample chamber to sample and image the sample.

[0051] Understandably, when axial zoom is enabled, the full-frame size is 2048*2048 pixels, determined by the relative motion between the light plate and the camera's shutter caused by the exciter's amplitude. With the exciter off, the image size is 2048*180 pixels. This is because with the exciter off, only the width of the light plate can be imaged. Since the light plate position is fixed, moving the sample chamber changes the relative position of the light plate and the sample, achieving optical tomography. However, to achieve higher resolution, the light plate is extremely thin, limiting the imaging width; therefore, previewing is not possible in this mode, and the image size is small. The camera's acquisition speed has a much wider range than the sample chamber's movement speed; therefore, the acquisition speed in this mode is primarily limited by the sample chamber's movement speed. However, at this point, the sampling speed is no longer limited by the zoom rate, but rather by the sample chamber's movement response rate and the camera's shutter scanning rate.

[0052] Understandably, the mode in which the exciter is turned off, and the camera adjusts the shutter speed according to the motion cycle of the sample chamber to sample and image the sample, is called high-speed sampling mode. It is evident that the imaging speed of high-speed sampling mode is more than 20 times that of standard sampling mode, making it more suitable for practical high-throughput, high-efficiency large-sample sampling and meeting the demand for large-volume imaging in a short time.

[0053] Please see Figure 4 , Figure 4 A schematic diagram illustrating the specific implementation process of another embodiment of step S3000 described above is shown. For example... Figure 4 As shown, the imaging method based on high-speed axial zoom technology in this application includes the following steps:

[0054] S3100, adjust the shutter speed so that the camera's line scan rate matches the movement frequency of the sample chamber.

[0055] Understandably, in high-speed sampling mode, the camera's sampling speed is no longer limited by the zoom rate, but primarily by the sample chamber's movement response rate and the camera's shutter scanning rate. Adjusting the shutter speed to match the camera's line scan rate with the sample chamber's movement frequency ensures that the camera can coordinate with the sample chamber's movement to acquire imaging data.

[0056] Understandably, to ensure the camera can perform imaging sampling in sync with the exciter's motion cycle, the camera's shutter speed is controlled via external modulation, making the camera's excitation signal period consistent with the sample chamber's movement frequency. This external modulation method for controlling the camera's shutter speed is existing technology and will not be elaborated upon here.

[0057] The S3200 performs sampling and imaging of a preset area of ​​the sample based on the movement frequency of the sample chamber.

[0058] Understandably, the camera sampling frequency is excited by an external square wave signal, which originates from DAQ control and is consistent with the sample chamber's movement frequency. Specifically, for every 1 nanometer movement of the sample chamber, the camera acquires one image according to a preset area (X2, Y2, W2, H2). In this case, the data acquisition speed is determined by the sample chamber's movement speed.

[0059] See Figure 5 , Figure 5 This is a schematic diagram of the structure of a light sheet microscope imaging system 400 based on high-speed axial zoom technology provided in this application embodiment. The entire process of the imaging method based on high-speed axial zoom technology provided in this application embodiment involves the following modules in the light sheet microscope imaging system 400 based on high-speed axial zoom technology: acquisition module 410, first sampling module 420 and second sampling module 430.

[0060] The acquisition module 410 is used to acquire the imaging requirement information of the sample and obtain the requirement signal.

[0061] The first sampling module 420 is used to turn on the exciter and drive the reflector to perform axial scanning of the sample on the sample chamber when the required signal is imaging preview. The camera adjusts the shutter speed in coordination with the motion cycle of the exciter and samples and images the sample.

[0062] The second sampling module 430 is used to turn off the exciter when the required signal is fast imaging, and the camera adjusts the shutter speed according to the motion cycle of the sample chamber and samples and images the sample.

[0063] It should be noted that the information interaction and execution process between the modules of the above-mentioned device are based on the same concept as the method embodiment of this application. For details on their specific functions and technical effects, please refer to the method embodiment section, and they will not be repeated here.

[0064] See Figure 6 , Figure 6 This is a schematic diagram of the structure of the first sampling module 420 and the second sampling module 430 in the light sheet microscope imaging system 400 based on high-speed axial zoom technology provided in this application embodiment. The specific component position relationship of the first sampling module 420 and the second sampling module 430 and the resulting technical effects are as described in step S1300 above, and will not be repeated here.

[0065] Figure 7 An electronic device 500 according to an embodiment of this application is shown. The electronic device 500 includes, but is not limited to:

[0066] Memory 501 is used to store programs;

[0067] The processor 502 is used to execute the program stored in the memory 501. When the processor 502 executes the program stored in the memory 501, the processor 502 is used to execute the above-mentioned imaging method based on high-speed axial zoom technology.

[0068] The processor 502 and the memory 501 can be connected via a bus or other means.

[0069] The memory 501, as a non-transitory computer-readable storage medium, can be used to store non-transitory software programs and non-transitory computer-executable programs, such as the imaging method based on high-speed axial zoom technology described in any embodiment of this application. The processor 502 implements the above-described imaging method based on high-speed axial zoom technology by running the non-transitory software program and instructions stored in the memory 501.

[0070] The memory 501 may include a program storage area and a data storage area. The program storage area may store the operating system and application programs required for at least one function. The data storage area may store the imaging method based on the high-speed axial zoom technology described above. Furthermore, the memory 501 may include high-speed random access memory and may also include non-transitory memory, such as at least one disk storage device, flash memory device, or other non-transitory solid-state storage device. In some embodiments, the memory 501 may optionally include memory remotely located relative to the processor 502, and these remote memories may be connected to the processor 502 via a network. Examples of such networks include, but are not limited to, the Internet, corporate intranets, local area networks, mobile communication networks, and combinations thereof.

[0071] The non-transient software program and instructions required to implement the imaging method based on high-speed axial zoom technology described above are stored in memory 501. When executed by one or more processors 502, the imaging method based on high-speed axial zoom technology provided in any embodiment of this application is executed.

[0072] This application also provides a storage medium storing computer-executable instructions for executing the above-described imaging method based on high-speed axial zoom technology.

[0073] In one embodiment, the storage medium stores computer-executable instructions that are executed by one or more control processors 502, such as one processor 502 in the aforementioned electronic device 500, which enable the one or more processors 502 to perform the imaging method based on high-speed axial zoom technology provided in any embodiment of this application.

[0074] The embodiments described above are merely illustrative. The units described as separate components may or may not be physically separate; that is, they may be located in one place or distributed across multiple network units. Some or all of the modules can be selected to achieve the purpose of this embodiment according to actual needs.

[0075] It will be understood by those skilled in the art that all or some of the steps and systems in the methods disclosed above can be implemented as software, firmware, hardware, and suitable combinations thereof. Some or all of the physical components can be implemented as software executed by a processor, such as a central processing unit, digital signal processor, or microprocessor, or as hardware, or as an integrated circuit, such as an application-specific integrated circuit. Such software can be distributed on a computer-readable medium, which can include computer storage media (or non-transitory media) and communication media (or transient media). As is known to those skilled in the art, the term computer storage media includes volatile and non-volatile, removable and non-removable media implemented in any method or technology for storing information (such as computer-readable instructions, data structures, program modules, or other data). Computer storage media includes, but is not limited to, RAM, ROM, EEPROM, flash memory or other memory technologies, CD-ROM, digital versatile disc (DVD) or other optical disc storage, magnetic cartridges, magnetic tape, disk storage or other magnetic storage devices, or any other medium that can be used to store desired information and is accessible to a computer. Furthermore, as is known to those skilled in the art, communication media typically include computer-readable instructions, data structures, program modules, or other data in modulated data signals such as carrier waves or other transmission mechanisms, and may include any information delivery medium.

Claims

1. An imaging method based on high speed axial zooming technique, characterized in that, The method includes: Obtain imaging requirement information for the sample to obtain the requirement signal; When the required signal is an imaging preview, the exciter is turned on and the mirror is driven to perform axial scanning of the sample on the sample chamber. The camera adjusts the shutter speed in coordination with the motion cycle of the exciter and samples and images the sample. When the required signal is for rapid imaging, the exciter is turned off, and the camera adjusts the shutter speed in accordance with the motion cycle of the sample chamber to sample and image the sample.

2. The imaging method based on high-speed axial zooming technology according to claim 1, characterized in that, The process of acquiring imaging requirement information for samples and obtaining a requirement signal includes: Obtain the imaging requirements information of the sample, including the imaging area, scanning thickness and imaging speed of the sample; The demand signal is obtained based on the imaging area, the scanning thickness, and the imaging speed.

3. The imaging method based on high-speed axial zoom technology according to claim 1, characterized in that, After obtaining the imaging requirement information of the sample and obtaining the requirement signal, the process further includes: The sample is placed on the sample chamber, wherein the sample chamber and the exciter are located on opposite sides of the polarization cube, and an objective lens is provided between the sample chamber and the exciter and the polarization cube, and the objective lens forms a symmetrical optical path on both sides of the polarization cube.

4. The imaging method based on high-speed axial zoom technology according to claim 3, characterized in that, A lens group is also provided between the sample chamber and the polarizing cube to correct spherical aberration and chromatic aberration generated by the mirror during its movement.

5. The imaging method based on high-speed axial zoom technology according to claim 1, characterized in that, The camera adjusts the shutter speed in conjunction with the motion cycle of the exciter and samples and images the sample, including: Adjust the shutter speed so that the line scan rate of the camera matches the motion cycle of the exciter; Based on the motion cycle of the exciter, a preset area of ​​the sample is sampled and imaged.

6. The imaging method based on high-speed axial zoom technology according to claim 5, characterized in that, Adjusting the shutter speed so that the camera's line scan rate matches the exciter's motion period includes: The shutter speed of the camera is controlled by external modulation, so that the excitation signal period of the camera is equal to the sum of the motion period of the exciter and the preset phase difference.

7. An imaging method based on high-speed axial zoom technology according to claim 3, characterized in that, The camera adjusts the shutter speed in conjunction with the motion cycle of the sample chamber and samples and images the sample, including: Adjust the shutter speed so that the line scan rate of the camera matches the movement frequency of the sample chamber; Based on the moving frequency of the sample chamber, a preset area of ​​the sample is sampled and imaged.

8. A light sheet microscope imaging system based on high-speed axial zoom technology, characterized in that, include: The acquisition module is used to acquire imaging requirement information of the sample and obtain the requirement signal; The first sampling module is used to turn on the exciter and drive the reflector to perform axial scanning of the sample on the sample chamber when the demand signal is an imaging preview. The camera adjusts the shutter speed in coordination with the motion cycle of the exciter and samples and images the sample. The second sampling module is used to turn off the exciter when the required signal is for rapid imaging, and the camera adjusts the shutter speed in coordination with the motion cycle of the sample chamber to sample and image the sample.

9. An electronic device, characterized in that, include: The memory, the processor, and the computer program stored in the memory and executable on the processor, wherein when the processor executes the computer program, it implements the imaging method based on high-speed axial zoom technology as described in any one of claims 1 to 7.

10. A computer-readable storage medium, characterized in that, The device contains a computer program that, when executed by a processor, implements the imaging method based on high-speed axial zoom technology as described in any one of claims 1 to 7.

Images