An anti-aging liquid collagen peptide composition and a preparation method thereof

Abstract

The present application relates to the technical field of health products, and particularly discloses an anti-aging liquid collagen peptide composition and a preparation method thereof.The anti-aging liquid collagen peptide composition is prepared by compounding liquid collagen peptide, a humectant, a sweetening agent, vitamin C, vitamin E, baizhi powder and zinc gluconate in a mass ratio of 88.482-90.686:0.1-0.5:9-10:0.1-0.5:0.002-0.004:0.1-0.5:0.012-0.014.The present application has the advantage of further improving the absorption of collagen peptide by human bodies and thus improving the anti-aging effect.

Description

Technical Field

[0001] This invention relates to the field of health products, and in particular to an anti-aging liquid collagen peptide composition and its preparation method. Background Technology

[0002] Collagen peptide products are mainly available in liquid and solid forms. Solid products dominate the market due to their ease of carrying and storage. However, despite their apparent advantages, solid collagen peptide products suffer from serious drawbacks, including difficulty in digestion and absorption, and low utilization rates. From a molecular structure perspective, the design of existing solid collagen peptide products fails to fully align with the intricate mechanisms of the human digestive system. Digestive enzymes in the human gastrointestinal tract struggle to precisely target and break down these products, resulting in their inability to be converted into easily absorbed small molecules within the gastrointestinal tract, significantly reducing their absorption and utilization rates.

[0003] The difficulty in digestion and absorption directly impacts the anti-aging effects of collagen peptide products. When the body cannot fully absorb the active ingredients, it struggles to improve collagen density, which is closely linked to the aging process. Therefore, the anti-aging effects actually experienced by consumers are far less significant than advertised, leading to lower satisfaction. Consequently, liquid collagen peptide products, due to their easier absorption, are gradually becoming the mainstream choice.

[0004] However, although existing liquid collagen peptide products are easier to absorb than solid collagen peptide products, the actual absorption rate of liquid collagen peptides by the human body is still low during actual use, making it difficult to fully utilize the collagen peptides. As a result, the health benefits are still not as expected, so there is still room for improvement. Summary of the Invention

[0005] In order to further improve the absorption of collagen peptides by the human body and thus enhance anti-aging effects, this application provides an anti-aging liquid collagen peptide composition and its preparation method.

[0006] In a first aspect, this application provides an anti-aging liquid collagen peptide composition, which adopts the following technical solution: An anti-aging liquid collagen peptide composition is formulated from liquid collagen peptides, moisturizers, sweeteners, vitamin C, vitamin E, angelica dahurica powder, and zinc gluconate in a mass ratio of 88.482-90.686:0.1-0.5:9-10:0.1-0.5:0.002-0.004:0.1-0.5:0.012-0.014.

[0007] By adopting the above technical solution and adding vitamin C, vitamin E, angelica root powder, and zinc gluconate, vitamin C and angelica root powder form a core absorption-enhancing duo. Vitamin C can activate the activity of proline hydroxylase and lysine hydroxylase, promoting the hydroxylation modification of collagen peptides, making them easier to form a stable triple helix structure and be absorbed by the intestines. The coumarin active ingredients in angelica root powder can improve intestinal mucosal permeability, providing a favorable environment for the transport of collagen peptides. At the same time, vitamin E reduces the oxidative loss of collagen peptides in the body by scavenging free radicals, and zinc compounds can regulate the activity of collagen synthesis-related enzymes. The two work synergistically to enhance the bioavailability of collagen peptides, thereby significantly improving the utilization rate of collagen peptides and thus significantly enhancing the anti-aging effect. The anti-aging sensation is more obvious, which well meets the needs of consumers.

[0008] Preferably, the liquid collagen peptide is a concentrated low-temperature collagen peptide solution.

[0009] By adopting the above technical solution and using concentrated low-temperature collagen peptide liquid, the concentration process can increase the effective content of collagen peptides per unit volume, ensuring sufficient efficacy. Low-temperature preparation (usually ≤60℃) can avoid peptide bond breakage and loss of activity caused by high temperature, and retain the small molecular structure of collagen peptides (mostly 1000-3000Da) to the greatest extent. When combined with vitamin C, vitamin E, angelica powder, and zinc gluconate, it can better improve bioavailability and make the anti-aging effect more significant.

[0010] Preferably, the sweetener is xylitol.

[0011] By adopting the above technical solution and adding xylitol, the taste can be effectively improved. Moreover, xylitol is stable and does not easily affect the efficacy of the composition, making it healthier.

[0012] Preferably, the humectant is Tremella fuciformis polysaccharide.

[0013] By adopting the above technical solution and adding Tremella polysaccharide, the skin moisturizing effect is enhanced, and the skin improvement effect is better.

[0014] Secondly, this application provides a method for preparing an anti-aging liquid collagen peptide composition, using the following technical solution: A method for preparing the above-mentioned anti-aging liquid collagen peptide composition includes the following steps: Step 1) Mix liquid collagen peptides, moisturizer, sweetener, vitamin C, vitamin E, angelica powder, and zinc gluconate to obtain a mixture; Step 2) The mixture is filtered through micropores, filled, and sterilized to obtain an anti-aging liquid collagen peptide composition.

[0015] By adopting the above technical solution, the anti-aging liquid collagen peptide composition obtained is of better quality and can play a significant anti-aging role.

[0016] Preferably, in step 2), the mixture is filtered through a 0.45-1μm filter membrane for microfiltration.

[0017] By adopting the above technical solution, microporous filtration through a 0.45-1μm filter membrane can trap impurities and undissolved particles while retaining small-molecule active ingredients. The filtered system is more uniform, reducing the impact of component aggregation on absorption. In synergy with the formula's absorption-enhancing design, it improves product purity and stability.

[0018] Preferably, in step 2), during sterilization, the temperature is raised to 105-110℃ and held for 15-20 minutes.

[0019] By adopting the above technical solution, sterilization at 105-110℃ achieves better sterilization effect, effectively extends the shelf life of the product, and does not easily damage the active ingredients, thus having higher economic value.

[0020] Thirdly, this application provides an application of an anti-aging liquid collagen peptide composition, employing the following technical solution: An application of the above-mentioned anti-aging liquid collagen peptide composition, wherein the anti-aging liquid collagen peptide composition can be used as a standalone product or added to a health supplement formulation for the preparation of health supplements.

[0021] By adopting the above technical solution, the anti-aging liquid collagen peptide composition is easily absorbed by the human body, has a high utilization rate of collagen peptides, and has a very significant anti-aging effect, which well meets the needs of consumers.

[0022] In summary, this application has the following beneficial effects: 1. By adding vitamin C, vitamin E, angelica dahurica powder, and zinc gluconate, this application can activate the activity of proline hydroxylase and lysine hydroxylase, promote the hydroxylation modification of collagen peptides, making them easier to form a stable triple helix structure and be absorbed by the intestines, and improve intestinal mucosal permeability, providing a favorable environment for the transport of collagen peptides. At the same time, it can regulate the activity of collagen synthesis-related enzymes, enhance the bioavailability of collagen peptides, and significantly improve the utilization rate of collagen peptides, thereby significantly enhancing the anti-aging effect and making the anti-aging experience more obvious, which well meets the needs of consumers.

[0023] 2. In this application, concentrated low-temperature collagen peptide solution is preferred. The concentration process can increase the effective content of collagen peptides per unit volume, ensuring sufficient efficacy. Low-temperature preparation can avoid peptide bond breakage and loss of activity caused by high temperature, and retain the small molecular structure of collagen peptides to the greatest extent. When combined with vitamin C, vitamin E, angelica powder and zinc gluconate, it can better improve bioavailability and make the anti-aging effect more significant. Detailed Implementation

[0024] The present application will be further described in detail below with reference to the embodiments.

[0025] Example 1

[0026] An anti-aging liquid collagen peptide composition, the preparation method of which includes the following steps: Step 1): Add 90.686g of liquid collagen peptides, 0.1g of Tremella fuciformis polysaccharide, 9g of xylitol, 0.1g of vitamin C, 0.002g of vitamin E, 0.1g of Angelica dahurica powder, and 0.012g of zinc gluconate into a mixing tank, stir at 100r / min for 10min, and mix to obtain a mixture.

[0027] Step 2), the mixture is filtered through a 0.45μm filter membrane, filled into containers, heated to 105℃, kept at that temperature for 15 minutes, and then cooled to obtain an anti-aging liquid collagen peptide composition.

[0028] The liquid collagen peptide is a concentrated, low-temperature collagen peptide liquid, purchased from Guangdong Huahuozhiguang Biotechnology Co., Ltd., product model: JYDBY-030, with a collagen peptide content of 30%.

[0029] Tremella polysaccharide was purchased from Shanghai Huiwen Biotechnology Co., Ltd.

[0030] Xylitol was purchased from Shandong Lvjian Biotechnology Co., Ltd.

[0031] Vitamin C was purchased from Shandong Tianli Pharmaceutical Co., Ltd.

[0032] Vitamin E is a compound nutritional fortifier (Vitamin E dry powder CWS / S) – 50% Vitamin E content was purchased from Xinchang Xinhecheng Vitamin Co., Ltd.

[0033] Angelica dahurica powder was purchased from Shaanxi Jiahe Biotechnology Co., Ltd., and is a water-soluble extract with an extraction ratio of 20:1.

[0034] Zinc gluconate was purchased from Zhengzhou Ruipu Bioengineering Co., Ltd.

[0035] Example 2

[0036] An anti-aging liquid collagen peptide composition, the preparation method of which includes the following steps: Step 1): Add 89.634g of liquid collagen peptides, 0.25g of Tremella fuciformis polysaccharide, 9.5g of xylitol, 0.3g of vitamin C, 0.003g of vitamin E, 0.3g of Angelica dahurica powder, and 0.013g of zinc gluconate into a mixing tank, stir at 150r / min for 13min, and mix to obtain a mixture.

[0037] Step 2), the mixture is filtered through a 0.8 μm filter membrane, filled into containers, heated to 108 °C, kept at that temperature for 18 min, and then cooled to obtain an anti-aging liquid collagen peptide composition.

[0038] The liquid collagen peptide is a concentrated, low-temperature collagen peptide liquid, purchased from Guangdong Huahuozhiguang Biotechnology Co., Ltd., product model: JYDBY-030, with a collagen peptide content of 30%.

[0039] Tremella polysaccharide was purchased from Shanghai Huiwen Biotechnology Co., Ltd.

[0040] Xylitol was purchased from Shandong Lvjian Biotechnology Co., Ltd.

[0041] Vitamin C was purchased from Shandong Tianli Pharmaceutical Co., Ltd.

[0042] Vitamin E is a compound nutritional fortifier (Vitamin E dry powder CWS / S) – 50% Vitamin E content was purchased from Xinchang Xinhecheng Vitamin Co., Ltd.

[0043] Angelica dahurica powder was purchased from Shaanxi Jiahe Biotechnology Co., Ltd., and is a water-soluble extract with an extraction ratio of 20:1.

[0044] Zinc gluconate was purchased from Zhengzhou Ruipu Bioengineering Co., Ltd.

[0045] Example 3

[0046] An anti-aging liquid collagen peptide composition, the preparation method of which includes the following steps: Step 1): Add 88.482g of liquid collagen peptides, 0.5g of Tremella fuciformis polysaccharide, 10g of xylitol, 0.5g of vitamin C, 0.004g of vitamin E, 0.5g of Angelica dahurica powder, and 0.014g of zinc gluconate into a mixing tank, stir at 200r / min for 15min, and mix to obtain a mixture.

[0047] Step 2): The mixture is filtered through a 1μm filter membrane, filled into containers, heated to 110℃, kept at that temperature for 20 minutes, and then cooled to obtain an anti-aging liquid collagen peptide composition.

[0048] The liquid collagen peptide is a concentrated, low-temperature collagen peptide liquid, purchased from Guangdong Huahuozhiguang Biotechnology Co., Ltd., product model: JYDBY-030, with a collagen peptide content of 30%.

[0049] Tremella polysaccharide was purchased from Shanghai Huiwen Biotechnology Co., Ltd.

[0050] Xylitol was purchased from Shandong Lvjian Biotechnology Co., Ltd.

[0051] Vitamin C was purchased from Shandong Tianli Pharmaceutical Co., Ltd.

[0052] Vitamin E is a compound nutritional fortifier (Vitamin E dry powder CWS / S) – 50% Vitamin E content was purchased from Xinchang Xinhecheng Vitamin Co., Ltd.

[0053] Angelica dahurica powder was purchased from Shaanxi Jiahe Biotechnology Co., Ltd., and is a water-soluble extract with an extraction ratio of 20:1.

[0054] Zinc gluconate was purchased from Zhengzhou Ruipu Bioengineering Co., Ltd.

[0055] Comparative Example 1 An anti-aging liquid collagen peptide composition, differing from Example 2 only in that: Ascorbate glucoside was used to replace vitamin C in equal amounts, astaxanthin was used to replace vitamin E in equal amounts, licorice powder was used to replace angelica powder in equal amounts, and zinc gluconate was used to replace zinc gluconate in equal amounts.

[0056] Ascorbate glucoside was purchased from Wuhan Smike Biotechnology Co., Ltd.

[0057] Astaxanthin was purchased from Hebei Hongtao Bioengineering Co., Ltd. The licorice powder was purchased from Baoji Liupanyun Biotechnology Co., Ltd. It is a water-soluble extract with an extraction ratio of 20:1.

[0058] Zinc glycine was purchased from Guangzhou Huayu Biotechnology Co., Ltd.

[0059] Comparative Example 2 An anti-aging liquid collagen peptide composition, differing from Example 2 only in that: Replace vitamin C with an equal amount of ascorbate glucoside.

[0060] Ascorbate glucoside was purchased from Wuhan Smike Biotechnology Co., Ltd.

[0061] Comparative Example 3 An anti-aging liquid collagen peptide composition, differing from Example 2 only in that: Replace vitamin E with an equal amount of astaxanthin.

[0062] Astaxanthin was purchased from Hebei Hongtao Bioengineering Co., Ltd. Comparative Example 4 An anti-aging liquid collagen peptide composition, differing from Example 2 only in that: Licorice powder was used to replace Angelica dahurica powder in an equal amount.

[0063] The licorice powder was purchased from Baoji Liupanyun Biotechnology Co., Ltd. It is a water-soluble extract with an extraction ratio of 20:1.

[0064] Comparative Example 5 An anti-aging liquid collagen peptide composition, differing from Example 2 only in that: Zinc glycinate was replaced with an equal amount of zinc gluconate.

[0065] Zinc glycine was purchased from Guangzhou Huayu Biotechnology Co., Ltd.

[0066] Comparative Example 6 An anti-aging liquid collagen peptide composition, differing from Example 2 only in that: Replace vitamin E, angelica root powder, and zinc gluconate with an equal amount of deionized water.

[0067] Comparative Example 7 An anti-aging liquid collagen peptide composition, differing from Example 2 only in that: Replace vitamin C, angelica powder, and zinc gluconate with an equal amount of deionized water.

[0068] Comparative Example 8 An anti-aging liquid collagen peptide composition, differing from Example 2 only in that: Replace vitamin C, vitamin E, and zinc gluconate with an equal amount of deionized water.

[0069] Comparative Example 9 An anti-aging liquid collagen peptide composition, differing from Example 2 only in that: Replace vitamin C, vitamin E, and angelica root powder with an equal amount of deionized water.

[0070] Comparative Example 10 An anti-aging liquid collagen peptide composition, differing from Example 2 only in that: Replace vitamin C, vitamin E, angelica root powder, and zinc gluconate with an equal amount of deionized water.

[0071] Comparative Example 11 An anti-aging liquid collagen peptide composition, differing from Example 2 only in that: Ascorbate glucoside was used to replace vitamin C in equal amounts, deionized water was used to replace vitamin E in equal amounts, angelica root powder was used to replace angelica root powder in equal amounts, and zinc gluconate was used to replace zinc gluconate in equal amounts.

[0072] Ascorbate glucoside was purchased from Wuhan Smike Biotechnology Co., Ltd.

[0073] Comparative Example 12 An anti-aging liquid collagen peptide composition, differing from Example 2 only in that: Replace vitamin C with an equal amount of deionized water, replace vitamin E with an equal amount of astaxanthin, replace angelica root powder with an equal amount of deionized water, and replace zinc gluconate with an equal amount of deionized water.

[0074] Astaxanthin was purchased from Hebei Hongtao Bioengineering Co., Ltd. Comparative Example 13 An anti-aging liquid collagen peptide composition, differing from Example 2 only in that: Replace vitamin C, vitamin E, angelica powder, and zinc gluconate with an equal amount of deionized water.

[0075] The licorice powder was purchased from Baoji Liupanyun Biotechnology Co., Ltd. It is a water-soluble extract with an extraction ratio of 20:1.

[0076] Comparative Example 14 An anti-aging liquid collagen peptide composition, differing from Example 2 only in that: Replace vitamin C, vitamin E, angelica root powder, and zinc gluconate with an equal amount of deionized water.

[0077] Zinc glycine was purchased from Guangzhou Huayu Biotechnology Co., Ltd.

[0078] Experiment 1 Antioxidant capacity test: Test subjects: Test samples prepared from the anti-aging liquid collagen peptide compositions of each embodiment and comparative example.

[0079] Test samples: 0.5g anti-aging liquid collagen peptide composition, 99.5g deionized water.

[0080] Test method: Place 2 mL of the test sample in a test tube, add 2 mL of 0.2 mmol / L DPPH radical solution, mix well, and react at room temperature in the dark for 30 min. Measure the absorbance at 517 nm. Use 2 mL of anhydrous ethanol instead of 2 mL of the test sample as a control group; use 2 mL of anhydrous ethanol instead of 2 mL of 0.2 mmol / L DPPH radical solution as a blank group. Calculate the DPPH radical scavenging rate of the test sample according to the following formula.

[0081] DPPH free radical scavenging rate (%) = [1 - (sample absorbance value - blank absorbance value) / control absorbance value)] × 100%.

[0082] Experiment 2 Collagen peptide absorption test: Samples: Anti-aging liquid collagen peptide compositions of various embodiments and comparative examples.

[0083] Instrument consumables: DERMTRVISI® Transdermal Skin OCT (Guangzhou Bainuotais Testing Technology Co., Ltd.), gavage needle (Zhongke Life Science Co., Ltd.), mouse β-galactosidase (βGAL) ELISA kit (Shanghai Jianglai Industrial Co., Ltd.), and ELISA reader (Meigu Molecular Instruments).

[0084] OCT stands for Optical Coherence Tomography.

[0085] Test Method: Mice were randomly selected and subjected to one week of acclimatization. Their estrous cycles were then observed using vaginal smears, and mice with normal estrous cycles were selected. These mice were then randomly divided into groups of six: a blank control group, a positive control group (β-nicotinamide mononucleotide), the sample group of Example 1, the sample group of Example 2, the sample group of Example 3, the sample group of Comparative Example 1, the sample group of Comparative Example 2, the sample group of Comparative Example 3, the sample group of Comparative Example 4, the sample group of Comparative Example 5, the sample group of Comparative Example 6, the sample group of Comparative Example 7, the sample group of Comparative Example 8, the sample group of Comparative Example 9, the sample group of Comparative Example 10, the sample group of Comparative Example 11, the sample group of Comparative Example 12, the sample group of Comparative Example 13, and the sample group of Comparative Example 14.

[0086] OCT was used to collect images and data on the density of skin collagen fibers in the abdominal marked skin of mice. At the same time, blood was collected from the abdominal vein of mice, and the blood β-galactosidase (βGAL) content was measured using a mouse β-galactosidase (βGAL) ELISA kit and a microplate reader. The data were used as the baseline value and recorded as D0 collagen fiber density.

[0087] The blank control group was fed normally, while the other groups were given daily gavage according to the corresponding samples. The gavage volume (based on the recommended dosage for a 60kg human body, referring to the body surface area method, with a coefficient of 10) was as follows: positive control group: 0.4mL (3mg / mL / D), example and comparative sample group: 0.4mL.

[0088] On day 14, OCT was used to collect images and data on the density of skin collagen fibers on the abdominal marked skin of mice, and the data was recorded as D14 collagen fiber density.

[0089] The detailed experimental data for Experiments 1-2 are shown in Table 1.

[0090] Table 1

[0091] According to the data in Table 1, all embodiments and comparative examples have good free radical scavenging effects and significant anti-aging effects. However, the collagen fiber density change rate (%) of each embodiment is significantly higher than that of each comparative example. It can be seen that the degree of absorption and utilization of each embodiment is significantly higher than that of each comparative example. Since each embodiment is more easily absorbed, the human body can obtain better anti-aging effects when ingesting the same amount of anti-aging liquid collagen peptide composition, the anti-aging sensation is more obvious, the anti-aging effect is faster, and it can better meet the needs of consumers.

[0092] This specific embodiment is merely an explanation of this application and is not intended to limit it. After reading this specification, those skilled in the art can make modifications to this embodiment without contributing any inventive step, but such modifications are protected by patent law as long as they fall within the scope of the claims of this application.

Claims

1. An anti-aging liquid collagen peptide composition, characterized in that: It is a compound made of liquid collagen peptides, moisturizers, sweeteners, vitamin C, vitamin E, angelica powder, and zinc gluconate in a mass ratio of 88.482-90.686:0.1-0.5:9-10:0.1-0.5:0.002-0.004:0.1-0.5:0.012-0.

014.

2. The anti-aging liquid collagen peptide composition according to claim 1, characterized in that: The liquid collagen peptide is a concentrated low-temperature collagen peptide solution.

3. The anti-aging liquid collagen peptide composition according to claim 2, characterized in that: The sweetener is xylitol.

4. The anti-aging liquid collagen peptide composition according to claim 3, characterized in that: The moisturizer is Tremella polysaccharide.

5. A method for preparing an anti-aging liquid collagen peptide composition according to any one of claims 1-4, characterized in that: Includes the following steps: Step 1) Mix liquid collagen peptides, moisturizer, sweetener, vitamin C, vitamin E, angelica powder, and zinc gluconate to obtain a mixture; Step 2) The mixture is filtered through micropores, filled, and sterilized to obtain an anti-aging liquid collagen peptide composition.

6. The method for preparing an anti-aging liquid collagen peptide composition according to claim 5, characterized in that: In step 2), the mixture is filtered through a 0.45-1μm filter membrane for microfiltration.

7. The method for preparing an anti-aging liquid collagen peptide composition according to claim 6, characterized in that: In step 2), during sterilization, the temperature is raised to 105-110℃ and held for 15-20 minutes.

8. The application of an anti-aging liquid collagen peptide composition according to any one of claims 1-4, characterized in that: The anti-aging liquid collagen peptide composition can be used as a standalone product or added to health supplement formulations for the preparation of health supplements.

Images