A plant extract compound for removing wrinkles and fine lines, and a preparation method and application thereof

Abstract

This invention provides a plant extract complex for wrinkle reduction and fine line removal, its preparation method, and its application, relating to the cosmetics field. The plant extract complex of this invention comprises the following raw materials in parts by weight: 3-7 parts of Centella asiatica extract, 2-4 parts of ginseng root extract, 2-3 parts of Astragalus membranaceus extract, 1-2 parts of grape seed extract, 1-2 parts of camellia flower extract, 0.5-1.5 parts of white tea extract, 0.5-1.5 parts of green tea extract, 0.5-1 part of Kakadu plum fruit extract, 0.3-1 part of holy basil leaf extract, 0.2-0.8 parts of broccoli bud extract, and 0.2-0.4 parts of guarana fruit extract. The product prepared by this invention possesses excellent anti-wrinkle and firming effects, good biocompatibility, and can effectively reduce wrinkles and fine lines.

Description

Technical Field

[0001] This invention relates to the field of cosmetics, and in particular to a plant extract complex for wrinkle reduction and fine line removal, its preparation method, and its application. Background Technology

[0002] Skin aging is a complex and progressive physiological process regulated by both endogenous factors (such as genetic background, hormonal changes, and decreased cell metabolic rate) and exogenous factors (such as ultraviolet radiation, environmental pollution, and oxidative stress damage). Wrinkles are one of the most direct macroscopic manifestations of skin aging, and their formation is closely related to the abnormal function of two key structural proteins in the skin—collagen and elastin. Collagen is the core supporting matrix that maintains the integrity and firmness of the skin structure. Type I collagen accounts for 80%–90% of the total collagen content in the skin and is mainly synthesized by fibroblasts in the dermis. Elastin is responsible for giving the skin the ability to recover its initial shape after being stretched or contracted. With age and the cumulative effect of external stimuli, the content of collagen fibers in the dermis decreases, and the fiber bundle structure becomes disordered. At the same time, exogenous stimuli such as ultraviolet radiation can activate matrix metalloproteinases (MMPs), accelerating the degradation and breakage of collagen, ultimately leading to loss of skin elasticity and the formation of wrinkles. Furthermore, advanced glycation end products (AGEs) generated by non-enzymatic glycation reactions further reduce collagen elasticity, exacerbating skin laxity and the development of wrinkles.

[0003] Currently, the main active ingredients used in cosmetics for anti-wrinkle and wrinkle reduction include retinoic acid and its derivatives, peptides, vitamin C, and hyaluronic acid. However, these ingredients generally have certain limitations: retinoic acid is highly irritating, easily causing adverse reactions such as skin redness, swelling, and peeling, and it also has poor photostability; while peptides are relatively safe, their large molecular weight results in low transdermal absorption, and they are easily inactivated in formulation systems; vitamin C is highly susceptible to oxidative degradation, making it difficult to ensure the stability of the formulation system. Therefore, developing safe, gentle, and effective anti-wrinkle and wrinkle-reducing active ingredients has become a research hotspot in the cosmetics industry.

[0004] In recent years, plant extracts have gained widespread attention in the field of anti-aging skincare due to their advantages such as natural origin, rich content of various bioactive components, and relatively high safety. Existing research shows that many plant extracts can exert antioxidant, anti-inflammatory, matrix metalloproteinase inhibition, and collagen synthesis-promoting effects, demonstrating good application potential in delaying skin aging.

[0005] However, currently available anti-wrinkle skincare compositions containing plant extracts still have several limitations: First, most existing compositions only target a single anti-wrinkle pathway (such as only promoting collagen synthesis or only scavenging free radicals for antioxidant effects), failing to achieve a systematic and comprehensive intervention in the skin aging process through multiple targets and pathways; Second, the active ingredients in plant extracts generally suffer from low transdermal absorption efficiency and limited bioavailability, thus hindering their full anti-wrinkle efficacy; Third, some existing compositions simply combine the components, failing to achieve the optimal synergistic anti-wrinkle effect; Fourth, there is still considerable room for improvement in balancing immediate wrinkle removal and long-lasting wrinkle reduction effects in existing compositions; Fifth, some active ingredients in plant extracts have poor water solubility, easily precipitating and forming sediment, thus limiting their practical application in cosmetic formulations.

[0006] Therefore, developing plant extract complexes with multi-target synergistic effects, excellent transdermal absorption, and superior wrinkle-reducing and fine line-fading efficacy remains a key technical problem that urgently needs to be solved in this field. Summary of the Invention

[0007] In view of this, the present invention proposes a plant extract complex for wrinkle removal and fine line reduction, its preparation method and application.

[0008] The technical solution of this invention is implemented as follows: A plant extract complex for wrinkle reduction and fine line reduction comprises the following raw materials in parts by weight: 3-7 parts Centella asiatica extract, 2-4 parts ginseng root extract, 2-3 parts Astragalus membranaceus extract, 1-2 parts grape seed extract, 1-2 parts camellia flower extract, 0.5-1.5 parts white tea extract, 0.5-1.5 parts green tea extract, 0.5-1 part Kakadu plum fruit extract, 0.3-1 part holy basil leaf extract, 0.2-0.8 parts broccoli bud extract, and 0.2-0.4 parts guarana fruit extract.

[0009] Furthermore, the plant extract complex comprises the following raw materials in parts by weight: 5.0 parts Centella asiatica extract, 3.0 parts ginseng root extract, 2.5 parts Astragalus membranaceus extract, 1.5 parts grape seed extract, 1.5 parts camellia flower extract, 1.0 part white tea extract, 1.0 part green tea extract, 0.8 parts Kakadu plum fruit extract, 0.7 parts holy basil leaf extract, 0.5 parts broccoli bud extract, and 0.3 parts guarana fruit extract.

[0010] This invention also provides a method for preparing a plant extract complex, comprising the following steps: (1) Mix Kakadu plum fruit extract, broccoli bud extract, guarana fruit extract, white tea extract, green tea extract and water, and stir at 300-400 rpm at 20-35℃ to dissolve them and obtain the aqueous phase; (2) Mix grape seed extract, holy basil leaf extract, camellia flower extract and polyol, and disperse by stirring at 400-600 rpm at 40-60℃ to obtain the oil phase; (3) Mix the oil phase obtained in step (2) with the aqueous phase obtained in step (1), add Centella asiatica extract, ginseng root extract, astragalus extract and emulsifier, homogenize at high speed at 5000~10000 rpm for 3~10 minutes, cool to room temperature, and you will get the product.

[0011] Furthermore, in step (1), the amount of water added is 5-10 times the total mass of the Kakadu plum fruit extract, broccoli bud extract, guarana fruit extract, white tea extract, and green tea extract.

[0012] Furthermore, the polyol mentioned in step (2) is one or more of butanediol, propylene glycol, glycerol, and pentanediol.

[0013] Furthermore, the amount of polyol used in step (2) is 4-8 times the total mass of grape seed extract, holy basil leaf extract, and camellia extract.

[0014] Furthermore, the emulsifier mentioned in step (3) is one or more of polysorbate-20, polysorbate-60, polysorbate-80, PEG-40 hydrogenated castor oil, PEG-60 hydrogenated castor oil, lecithin, hydrogenated lecithin, sucrose stearate, and sucrose palmitate.

[0015] Furthermore, the amount of emulsifier added is 5% to 30% of the total weight of the oil phase obtained in step (2).

[0016] The plant extract complex described in any one of the present invention is used in the preparation of anti-wrinkle and wrinkle-reducing cosmetics.

[0017] Furthermore, the plant extract complex is used in the preparation of anti-wrinkle and wrinkle-reducing serums, creams, or lotions.

[0018] Compared with the prior art, the beneficial effects of the present invention are: The plant extract complex of this invention is based on Centella asiatica extract, ginseng root extract, Astragalus membranaceus extract, grape seed extract, camellia extract, white tea extract, and green tea extract, with the addition of Kakadu plum fruit extract, holy basil leaf extract, broccoli bud extract, and guarana fruit extract. The optimized ratio achieves multi-target synergistic effects, excellent transdermal absorption, and excellent wrinkle-reducing and fine line-fading effects.

[0019] The product prepared by this invention has excellent anti-wrinkle and firming effects, and can effectively remove wrinkles and reduce fine lines. Tests showed that after using the product, the average change rate of the skin elasticity parameter R2 in subjects was higher than 41%; the upregulation rate of the expression of the type I collagen encoding gene COL1A1 was higher than 84%, and the upregulation rate of the expression of the elastin encoding gene ELN was higher than 71%. Furthermore, all samples prepared by this invention exhibited good biocompatibility. Detailed Implementation

[0020] Unless otherwise specified, the experimental methods used in the embodiments of this invention are conventional methods; Unless otherwise specified, all materials and reagents used in the embodiments of this invention are commercially available.

[0021] All extracts used in this invention are commercially available raw materials.

[0022] Table 1 Source of Raw Materials

[0023] Among them, the Astragalus extract is an extract of Astragalus membranaceus.

[0024] Example 1 Formula: Centella asiatica extract 5.0g, ginseng root extract 3.0g, astragalus extract 2.5g, grape seed extract 1.5g, camellia flower extract 1.5g, white tea extract 1.0g, green tea extract 1.0g, Kakadu plum fruit extract 0.8g, holy basil leaf extract 0.7g, broccoli bud extract 0.5g, guarana fruit extract 0.3g.

[0025] Preparation steps: (1) Mix 3.6g of Kakadu plum fruit extract, broccoli bud extract, guarana fruit extract, white tea extract, and green tea extract with 25g of purified water (the amount of purified water is about 6.9 times the total mass of the aqueous phase extract), and stir at 300 rpm for 20 minutes at 25°C until completely dissolved to obtain the aqueous phase; (2) Mix grape seed extract, holy basil leaf extract, and camellia flower extract (total 3.7g) with 22g of butylene glycol (the amount of butylene glycol is about 5.9 times the total mass of the oil phase extract), and stir at 500 rpm for 15 minutes at 50°C until uniformly dispersed to obtain the oil phase; (3) The oil phase obtained in step (2) is slowly added to the aqueous phase obtained in step (1) under stirring at 500 rpm. At the same time, Centella asiatica extract, ginseng root extract, astragalus extract (total 10.5g) and PEG-40 hydrogenated castor oil 6.0g (the amount of PEG-40 hydrogenated castor oil added accounts for about 23.3% of the total mass of the oil phase) are added. The mixture is homogenized at 8000 rpm for 5 minutes, cooled to room temperature, and purified water is added to a total weight of 100g. The mixture is stirred evenly to obtain the plant extract complex.

[0026] Example 2 Formula: Centella asiatica extract 7.0g, ginseng root extract 2.0g, astragalus extract 2.0g, grape seed extract 2.0g, camellia flower extract 2.0g, white tea extract 0.5g, green tea extract 1.5g, Kakadu plum fruit extract 1.0g, holy basil leaf extract 0.3g, broccoli bud extract 0.8g, guarana fruit extract 0.2g.

[0027] Preparation steps: (1) Mix Kakadu plum fruit extract, broccoli bud extract, guarana fruit extract, white tea extract, and green tea extract (total 2.5g) with 25g purified water (the amount of purified water is 10 times the total mass of the aqueous phase extract), and stir at 400 rpm for 30 minutes at 20℃ until completely dissolved to obtain the aqueous phase; (2) Mix grape seed extract, holy basil leaf extract, and camellia flower extract (total 4.3g) with 17g of glycerin (the amount of glycerin is about 4.0 times the total mass of the oil phase extract), and stir at 400 rpm for 10 minutes at 60℃ until uniformly dispersed to obtain the oil phase; (3) Slowly add the oil phase obtained in step (2) to the aqueous phase obtained in step (1), and add Centella asiatica extract, ginseng root extract, astragalus extract (total 11.0g) and 1.1g of sucrose stearate (the amount of sucrose stearate added accounts for about 5.2% of the total mass of the oil phase). Homogenize at 5000 rpm for 10 minutes, cool to room temperature, and add purified water to 100g to obtain the plant extract complex.

[0028] Example 3 Formula: Centella asiatica extract 3.0g, ginseng root extract 4.0g, astragalus extract 3.0g, grape seed extract 1.0g, camellia flower extract 1.0g, white tea extract 1.5g, green tea extract 0.5g, Kakadu plum fruit extract 0.5g, holy basil leaf extract 1.0g, broccoli bud extract 0.2g, guarana fruit extract 0.4g.

[0029] Preparation steps: (1) Mix Kakadu plum fruit extract, broccoli bud extract, guarana fruit extract, white tea extract, and green tea extract (total 2.6g) with 13g of purified water (the amount of purified water is 5 times the total mass of the aqueous extract), and stir at 300 rpm for 15 minutes at 35°C until completely dissolved to obtain the aqueous phase; (2) Mix grape seed extract, holy basil leaf extract, and camellia flower extract (total 3.0g) with 24g of propylene glycol (the amount of propylene glycol is 8 times the total mass of the oil phase extract), and stir at 600 rpm for 20 minutes at 40℃ until uniformly dispersed to obtain the oil phase; (3) Slowly add the oil phase obtained in step (2) to the aqueous phase obtained in step (1), and simultaneously add Centella asiatica extract, ginseng root extract, astragalus extract (total 10.0g) and polysorbate-60 8.1g (the amount of polysorbate-60 added accounts for 30% of the total mass of the oil phase). Homogenize at 10000 rpm for 3 minutes, cool to room temperature, and add purified water to 100g to obtain the plant extract complex.

[0030] Example 4 Formula: Centella asiatica extract 6.0g, ginseng root extract 3.5g, astragalus extract 2.8g, grape seed extract 1.2g, camellia flower extract 1.8g, white tea extract 1.2g, green tea extract 1.2g, Kakadu plum fruit extract 0.6g, holy basil leaf extract 0.5g, broccoli bud extract 0.6g, guarana fruit extract 0.35g.

[0031] Preparation steps: (1) Mix Kakadu plum fruit extract, broccoli bud extract, guarana fruit extract, white tea extract, and green tea extract (total 2.75g) with 20g purified water (the amount of purified water is about 7.3 times the total mass of the aqueous phase extract), and stir at 350 rpm for 25 minutes at 28℃ until completely dissolved to obtain the aqueous phase; (2) Mix grape seed extract, holy basil leaf extract, and camellia flower extract (total 3.5g) with 17g of a mixed polyol consisting of 10g butylene glycol and 7g pentylene glycol (the total amount of polyol is about 4.9 times the total mass of the oil phase extract), and stir at 500 rpm for 15 minutes at 55°C until uniformly dispersed to obtain the oil phase; (3) Slowly add the oil phase obtained in step (2) to the aqueous phase obtained in step (1), and simultaneously add Centella asiatica extract, ginseng root extract, astragalus extract (total 12.3g), lecithin 4.1g and PEG-40 hydrogenated castor oil 2.0g of composite emulsifier 6.1g (the total amount of emulsifier added accounts for about 29.8% of the total mass of the oil phase). Homogenize at 7000 rpm for 6 minutes, cool to room temperature, and add purified water to 100g to obtain the plant extract complex.

[0032] Example 5 Formula: Centella asiatica extract 4.0g, ginseng root extract 2.5g, astragalus extract 2.2g, grape seed extract 1.8g, camellia flower extract 1.3g, white tea extract 0.8g, green tea extract 0.8g, Kakadu plum fruit extract 0.9g, holy basil leaf extract 0.8g, broccoli bud extract 0.4g, guarana fruit extract 0.25g.

[0033] Preparation steps: (1) Mix Kakadu plum fruit extract, broccoli bud extract, guarana fruit extract, white tea extract, and green tea extract (total 2.15g) with 18g of purified water (the amount of purified water is about 8.4 times the total mass of the aqueous phase extract), and stir at 350 rpm for 20 minutes at 30℃ until completely dissolved to obtain the aqueous phase; (2) Mix grape seed extract, holy basil leaf extract, and camellia flower extract (total 3.9g) with 20g of a mixed solvent containing 10g of butylene glycol, 5g of glycerin, and 5g of caprylic / capric triglyceride (the total amount of solvent is about 5.1 times the total mass of the oil phase extract), and stir at 500 rpm for 15 minutes at 50°C until uniformly dispersed to obtain the oil phase; (3) Slowly add the oil phase obtained in step (2) to the aqueous phase obtained in step (1), and simultaneously add Centella asiatica extract, ginseng root extract, astragalus extract (total 8.7g), sucrose stearate 2.5g and polysorbate-60 2.5g composite emulsifier 5.0g (the amount of emulsifier added accounts for about 20.9% of the total mass of the oil phase), homogenize at high speed at 9000 rpm for 4 minutes, cool to room temperature, and add purified water to 100g to obtain the plant extract complex.

[0034] Comparative Example 1 Formula: Centella asiatica extract 5.0g, ginseng root extract 3.0g, astragalus extract 2.5g, grape seed extract 2.0g, camellia flower extract 2.0g, white tea extract 1.8g, green tea extract 1.5g. The missing portions of Kakadu plum fruit extract, holy basil leaf extract, broccoli bud extract, and guarana fruit extract were supplemented by grape seed extract, camellia flower extract, white tea extract, and green tea extract in proportion, maintaining the total mass of all extracts consistent with Example 1.

[0035] Preparation steps: (1) Mix white tea extract, green tea extract (total 3.3g) with 25g water and stir at 300 rpm for 20 minutes at 25℃ to obtain the aqueous phase; (2) Mix grape seed extract, camellia extract (4.0g in total) with 22g of butylene glycol and stir at 500rpm for 15 minutes at 50℃ to obtain the oil phase; (3) Add the oil phase to the aqueous phase, add Centella asiatica extract, ginseng root extract, astragalus extract (total 10.5g) and PEG-40 hydrogenated castor oil 6.0g, homogenize at 8000 rpm for 5 minutes, cool to room temperature, add purified water to 100g, and the product is obtained.

[0036] Comparative Example 2 Formula: Centella asiatica extract 5.0g, ginseng root extract 3.0g, astragalus extract 2.5g, grape seed extract 1.5g, green tea extract 3.5g, Kakadu plum fruit extract 0.8g, holy basil leaf extract 0.7g, broccoli bud extract 0.5g, guarana fruit extract 0.3g. Camellia flower extract and white tea extract, if missing, are replaced with an equal amount of green tea extract. The total weight of all extracts remains consistent with Example 1.

[0037] The preparation steps are basically the same as in Example 1.

[0038] Comparative Example 3 (Excessive amount of Kakadu plum fruit extract added) Formula: Centella asiatica extract 3.7g, ginseng root extract 3.0g, astragalus extract 2.5g, grape seed extract 1.5g, camellia flower extract 1.5g, white tea extract 0.8g, green tea extract 0.8g, Kakadu plum fruit extract 2.5g, holy basil leaf extract 0.7g, broccoli bud extract 0.5g, guarana fruit extract 0.3g. The remaining extracts are formulated according to the proportions in Example 1, with any excess Kakadu plum fruit extract deducted from the Centella asiatica extract. The total mass of all extracts is maintained consistent with that in Example 1.

[0039] The preparation steps are the same as in Example 1.

[0040] Comparative Example 4 (no stepwise preparation process was used – all extracts were directly blended) The same raw material ratio as in Example 1 was used for preparation, except that the step-by-step process of steps (1), (2), and (3) was not used. Instead, all the dry powder of the extract, 22g of butylene glycol, and 6.0g of PEG-40 hydrogenated castor oil were added to 50g of purified water at one time, homogenized at 8000 rpm for 5 minutes at 50°C, cooled to room temperature, and purified water was added to bring the total to 100g.

[0041] Results: The resulting mixture showed obvious clumping and oil-water separation, and some extracts precipitated as particles without pre-dispersion.

[0042] Experimental Example 1 This study randomly selected participants aged 35-45 years and divided them into 8 groups of 10 participants each. Participants in each group applied the cosmetics prepared in Examples 1-5 and Comparative Examples 1-3 to the left and right sides of their faces, respectively, twice daily (morning and evening), for a period of 28 days. Participants were required to refrain from using any other products that maintain skin elasticity or any cosmetics that might interfere with the results, and to maintain their original dietary habits throughout the entire study.

[0043] The skin elasticity of the zygomatic region of the subjects was measured using a Cutometer MPA580 skin elasticity tester on day 0 (baseline) and day 28 of the experiment. The probe was used to measure the same area three times, and the average value of the results was used for subsequent analysis. This study selected the test parameter R² (the ratio of skin rebound Ua under no negative pressure to the maximum skin stretch Uf under negative pressure) to evaluate skin elasticity; a R² closer to 1 indicates better skin elasticity.

[0044] Wherein, the average change in R² = the R² value on day 28 R² value on day 0; R² average rate of change = R² average change / R² value on day 0.

[0045] See Table 2 for the results of the skin elasticity test.

[0046] Table 2 Results of Skin Elasticity Test

[0047] The results showed that the average change rate of skin R2 in Examples 1-5 was higher than 41%, indicating that the products prepared in Examples 1-5 of this invention have excellent anti-wrinkle effects, with Example 1 showing the best anti-wrinkle effect. The average change rate of skin R2 in Comparative Examples 1-3 was lower than 34%, which was significantly lower than that in Example 1.

[0048] Experimental Example 2 Determination of the upregulation rate of type I collagen (COL1A1) and elastin (ELN) expression in human dermal fibroblasts.

[0049] Test Principle: Fibroblasts synthesize and secrete collagen and elastin, forming collagen fibers, reticular fibers, and elastic fibers. They also synthesize and secrete extracellular matrix components such as glycosaminoglycans and glycoproteins. Type I and Type III collagen are core components of the dermal extracellular matrix; in the skin tissue of infants and adolescents, Type I collagen accounts for approximately 70%. The formation of skin wrinkles is closely related to the normal synthesis and expression levels of collagen. Elastin, distributed in the extracellular matrix, plays a crucial role in maintaining skin elasticity; abnormal expression is a significant factor in causing skin laxity and sagging. Ultraviolet radiation induces skin roughness, thickening, and loss of elasticity, ultimately leading to wrinkle formation. Weakened skin barrier function is closely related to changes in hemidesmosome structure and downregulation of various collagen proteins (such as Type I collagen) in the dermal-epidermal junction region. This experiment uses human dermal fibroblasts as a research model. After cell treatment, the relative expression levels of COL1A1 and ELN genes are detected to further evaluate whether the tested samples possess anti-wrinkle and firming effects.

[0050] Experimental steps: (1) Seed cells in 6-well culture plates at a seeding density of 3 × 10⁶ cells / well. 5 Each sample was placed in a 37°C, 5% CO2 incubator for 24 h.

[0051] (2) After incubation, discard the original culture medium and wash with D-Hanks buffer 1-2 times. Then, the sample group and the model control group were subjected to UVA irradiation treatment with an irradiation dose of 9 J / cm².

[0052] (3) After the modeling was completed, the culture medium of the normal control group and the model control group was replaced with fresh complete culture medium, and the sample group well plate was replaced with fresh culture medium containing the target sample. The plates were then placed in an incubator at 37°C and 5% CO2 for 24 h.

[0053] (4) Total RNA was extracted from cells in each experimental group and reverse transcribed to synthesize cDNA. The expression levels of the internal reference gene β-actin and the target gene were detected by real-time quantitative PCR (qPCR).

[0054] (5) Using β-actin as an internal reference for gene expression detection, the relative RNA expression level of the target gene was calculated.

[0055] relative RNA expression level = 2ΔΔC(t); In the formula, ΔC(t) = ΔC(t) model control group - ΔC(t) sample group; ΔC(t) = C(t)target gene_C(t)β-actin; Upward adjustment rate (%) = (P2 - P1) ÷ P1 × 100% In the formula, P represents the relative expression levels of COL1A1 and ELN genes, where P2 is the sample group and P1 is the model control group.

[0056] Table 3. Upregulation rate of type I collagen and elastin expression

[0057] The results showed that the upregulation rate of type I collagen (COL1A1) expression in Examples 1-5 was higher than 84%, and the upregulation rate of elastin (ELN) expression was higher than 71%, further demonstrating the good anti-wrinkle and firming effect of the product of the present invention. Compared with Example 1, the upregulation rates of both proteins in Comparative Examples 1-3 were significantly reduced, which further confirms the significant advantages of the formulation ratio used in the examples of the present invention.

[0058] Experiment Example 3 - Safety Evaluation Experiment This study recruited 56 female subjects aged 25-35 years. All subjects had no history of cosmetic or contact allergies. Each subject underwent closed patch testing with samples prepared in Examples 1-5 and Comparative Examples 1-3, respectively. Before the test, the subject's back skin was cleaned. The patch applicator loaded with the test product was fixed to the selected test area on the back using low-irritation medical tape. After fixation, the patch applicator was gently pressed with the fingertip to ensure even adhesion to the skin surface. The closed patch application time was 48 hours. Subjects were required to keep the patch area dry during the 48-hour application period, avoid strenuous exercise, strictly prohibit scratching the test area, and avoid prolonged exposure of the test area to sunlight. After 48 hours, the patch applicator was removed and the test area was marked. After 30 minutes, when the skin indentation had completely faded, the skin allergic reaction was assessed under sufficient and homogeneous light. The grading criteria for adverse skin reactions are detailed in Table 4, and the safety evaluation results are detailed in Table 5.

[0059] Table 4. Grading Standards for Adverse Skin Reactions

[0060] Table 5 Safety Evaluation Results

[0061] The results showed that all subjects in each group had negative reactions and no adverse effects were observed, indicating that the samples prepared in Examples 1-5 and Comparative Examples 1-3 of this invention all have good safety.

[0062] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention.

Claims

1. A plant extract complex for use in the treatment of wrinkles and fine lines, characterized in that, The ingredients include the following parts by weight: Centella asiatica extract 3-7 parts, ginseng root extract 2-4 parts, astragalus extract 2-3 parts, grape seed extract 1-2 parts, camellia flower extract 1-2 parts, white tea extract 0.5-1.5 parts, green tea extract 0.5-1.5 parts, Kakadu plum fruit extract 0.5-1 part, holy basil leaf extract 0.3-1 part, broccoli bud extract 0.2-0.8 parts, and guarana fruit extract 0.2-0.4 parts.

2. The plant extract complex according to claim 1, characterized in that, The ingredients include the following parts by weight: Centella asiatica extract 5.0 parts, ginseng root extract 3.0 parts, astragalus extract 2.5 parts, grape seed extract 1.5 parts, camellia flower extract 1.5 parts, white tea extract 1.0 part, green tea extract 1.0 part, Kakadu plum fruit extract 0.8 parts, holy basil leaf extract 0.7 parts, broccoli bud extract 0.5 parts, and guarana fruit extract 0.3 parts.

3. Process for the preparation of a plant extract complex according to any one of claims 1 or 2, characterized in that, Includes the following steps: (1) Mix Kakadu plum fruit extract, broccoli bud extract, guarana fruit extract, white tea extract, green tea extract and water, and stir at 300-400 rpm at 20-35℃ to dissolve them and obtain the aqueous phase; (2) Mix grape seed extract, holy basil leaf extract, camellia flower extract and polyol, and disperse by stirring at 400-600 rpm at 40-60℃ to obtain the oil phase; (3) Mix the oil phase obtained in step (2) with the aqueous phase obtained in step (1), add Centella asiatica extract, ginseng root extract, astragalus extract and emulsifier, homogenize at high speed at 5000~10000 rpm for 3~10 minutes, cool to room temperature, and you will get the product.

4. The method of claim 3, wherein the plant extract complex is prepared by the steps of: In step (1), the amount of water added is 5-10 times the total mass of the Kakadu plum fruit extract, broccoli bud extract, guarana fruit extract, white tea extract, and green tea extract.

5. The method for preparing the plant extract complex according to claim 3, characterized in that, The polyol mentioned in step (2) is one or more of butanediol, propylene glycol, glycerol, and pentanediol.

6. The method for preparing the plant extract complex according to claim 3, characterized in that, The amount of polyol used in step (2) is 4-8 times the total mass of grape seed extract, holy basil leaf extract, and camellia extract.

7. The method for preparing the plant extract complex according to claim 3, characterized in that, The emulsifier mentioned in step (3) is one or more of the following: polysorbate-20, polysorbate-60, polysorbate-80, PEG-40 hydrogenated castor oil, PEG-60 hydrogenated castor oil, lecithin, hydrogenated lecithin, sucrose stearate, and sucrose palmitate.

8. The method for preparing the plant extract complex according to claim 3, characterized in that, The amount of emulsifier added is 5% to 30% of the total weight of the oil phase obtained in step (2).

9. The use of the plant extract complex according to any one of claims 1-2 in the preparation of anti-wrinkle and wrinkle-reducing cosmetics.

10. The application according to claim 9, characterized in that, The plant extract complex is used in the preparation of anti-wrinkle and wrinkle-reducing serums, creams, or lotions.

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