Preparation method and application of intestinal physical mouse control agent

Abstract

The present application relates to the technical field of rodent control, and particularly relates to a preparation method and application of an intestinal physical rodent control agent, and the preparation method comprises the following steps: uniformly mixing base powder and additives to obtain mixed base material; adding high-molecular polyacrylic acid sodium into the mixed base material and uniformly mixing to obtain mixed material; feeding the mixed material into a feed pelletizer to be extruded into granular bait; and naturally air-drying the granular bait and then sealing and storing the granular bait. The base powder is prepared by corn powder, cooked peanut powder, soybean meal and potato starch according to a ratio of 3:3:2:2, and yeast extract powder and white sugar are added. The molecular weight of the high-molecular polyacrylic acid sodium is 8000W, and the adding concentration is 10% to 20% of the total weight of the base powder. After the agent prepared by the present application is eaten by rodents, the polyacrylic acid sodium absorbs water and expands in the digestive tract, physically blocks the intestinal tract, and achieves the effect of rodent control. The agent has the advantages of ecological friendliness, no drug resistance, excellent palatability, simple preparation process and the like, and is suitable for green rodent control in grasslands.

Description

Technical Field

[0001] This invention relates to the field of rodent control technology, and in particular to a method for preparing and applying an intestinal physical rodent control agent. Background Technology

[0002] Grassland ecosystems are among the world's most important terrestrial ecosystems, possessing irreplaceable ecological, productive, and economic value. However, influenced by both natural and anthropogenic factors, vast areas of grassland globally are experiencing varying degrees of degradation, with rodent outbreaks being a significant driving force behind this deterioration. Furthermore, rodents are important vectors for numerous zoonotic diseases, carrying and transmitting pathogens such as plague, hemorrhagic fever, leptospirosis, and echinococcosis, posing a potential threat to public health. Therefore, how to safely and efficiently control rodent population density has become one of the urgent technical challenges to be addressed in the field of grassland ecological protection.

[0003] In the field of rodenticide technology, the active ingredients of rodenticides disclosed in the prior art mainly include anticoagulant rodenticides, sterilizing agents, and a few biological agents. Among them, anticoagulant rodenticides are the most widely used type of rodenticide due to their relatively low toxicity and the availability of antidotes. However, long-term use has shown that this type of chemical rodenticide has the following inherent defects: (1) the chemical drugs have a long residual period in soil and water, which can easily cause environmental pollution; (2) they can easily produce bioaccumulation effects in predatory animals through the food chain, which poses a potential threat to non-target organisms; (3) rodents are prone to developing drug resistance after long-term exposure, which leads to a year-by-year decline in the control effect, requiring continuous increase in drug concentration, forming a vicious cycle.

[0004] In summary, the inherent drawbacks of existing chemical rodenticides, such as environmental residues, bioaccumulation, and resistance, are becoming increasingly prominent, necessitating the development of a novel alternative technology based on a non-chemical mechanism that is both eco-friendly and highly effective in rodent control. Summary of the Invention

[0005] The purpose of this invention is to provide a method for preparing and applying an intestinal physical rodent control agent, aiming to solve the technical problems of existing chemical rodenticides, which pose risks of environmental pollution, bioaccumulation, and drug resistance, and are difficult to meet the needs of ecological rodent control.

[0006] To achieve the above objectives, the present invention provides a method for preparing an intestinal physical rodent control agent, comprising the following steps: Prepare the palatability-enhancing base material: Mix the base powder and additives evenly according to the preset ratio to obtain the mixed base material; Adding active ingredients: Add sodium polyacrylate to the mixed base material and mix evenly to obtain a mixed material; Extrusion granulation: The mixture is fed into a feed pellet mill and extruded into pelleted feed. Drying and sealing: After the granular bait is air-dried naturally, it is sealed and stored to obtain the intestinal physical rodent control agent.

[0007] In the step of preparing the palatable base material, the base powder is composed of corn flour, roasted peanut powder, soybean meal and potato starch.

[0008] In the step of preparing the palatable base material, the ratio of corn flour, cooked peanut flour, soybean meal and potato starch is 3:3:2:2.

[0009] In the step of preparing the palatable base material, the additives include yeast extract and white sugar. The amount of yeast extract added is 1% of the total weight of the base powder, and the amount of white sugar added is 6% of the total weight of the base powder.

[0010] In the step of adding active ingredients, the molecular weight of the high molecular weight sodium polyacrylate is 8000W.

[0011] In the step of adding active ingredients, the concentration of the added sodium polyacrylate is 10% to 20% of the total weight of the base powder.

[0012] The present invention also provides the application of an intestinal physical rodent control agent prepared by the preparation method of the intestinal physical rodent control agent described above in the prevention and control of rodent pests.

[0013] The present invention provides a method for preparing and applying an intestinal physical rodent control agent, which has the following advantages and advancements compared to the prior art: (1) Physical rodent control, ecological safety. This invention uses sodium polyacrylate as the core active ingredient. After rodents ingest it, it absorbs water and swells in the intestines to form a hydrogel, physically blocking the intestines and causing the rodents to die due to intestinal obstruction, dehydration, and nutrient absorption disorders. This process does not involve any chemical poisoning reaction, has no risk of drug resistance, is safe for non-target organisms, and the degradation products are water and carbon dioxide, making it environmentally friendly.

[0014] (2) Precise intestinal targeting. This invention selects sodium polyacrylate with a specific molecular weight (8000W) that has a suitable water absorption rate and volume (about 40 times) in a gastric acid environment (pH≈3), avoiding premature water absorption and swelling in the stomach that could lead to bloating and loss of appetite. This achieves a physical blockage effect of continuous water absorption and swelling in the intestine, thus improving the efficiency of rodent control.

[0015] (3) Excellent palatability. This invention optimizes the ratio of feed additives, using natural ingredients such as corn flour, peanut flour, and soybean meal as the base, supplemented with yeast extract and white sugar to adjust the flavor, which significantly improves the feeding willingness of rodents and ensures the effective intake of the drug.

[0016] (4) The preparation process is simple. The present invention can be produced using conventional feed processing equipment, with few steps and simple operation, making it suitable for industrial-scale production. Attached Figure Description

[0017] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0018] Figure 1 This is a flowchart of the preparation method of the intestinal physical rodent control agent provided in Example 1 of the present invention.

[0019] Figure 2 This is a graph showing the water absorption test results of sodium polyacrylate of different molecular weights provided by this invention in an acidic environment with pH=3.

[0020] Figure 3 These are the molding effect diagrams of the bait prepared in Examples 1 and 2 of the present invention, wherein (a) is bait containing 10% sodium polyacrylate and (b) is bait containing 20% ​​sodium polyacrylate.

[0021] Figure 4 The results of the effect of the intestinal physical rodent control agent provided by the present invention on the daily food intake of mice are shown in the figure, where (a) is the curve of the change in daily food intake of mice and (b) is the box plot of the analysis of variance of food intake.

[0022] Figure 5 The results of the effect of the intestinal physical rodent control agent provided by the present invention on the weight change of mice are shown in the figure, where (a) is the daily weight change curve of mice and (b) is the box plot of the final weight variance analysis.

[0023] Figure 6 This is an analysis diagram of the effects of the intestinal physical rodenticide provided by the present invention on the organ indices of mice, where a is the heart, b is the liver, c is the spleen, d is the lung, and e is the kidney.

[0024] Figure 7 These are histopathological sections of mice treated with the intestinal physical rodent control agent provided by this invention (black arrows indicate inflammatory cell infiltration, green arrows indicate muscle layer edema), where a is the colon, b is the ileum, and c is the kidney.

[0025] Figure 8 This is a comparative graph showing the effect of the intestinal physical control rodent agent provided by the present invention on the colon length of mice, where a is the CK group, b is the A group, c is the B group, and d is a box plot of colon length.

[0026] Figure 9 This is an analysis diagram of the effects of the intestinal physical rodent control agent provided by the present invention on the antioxidant indicators of mouse liver, where (a) is the total antioxidant capacity, (b) is the reduced glutathione level, (c) is the glutathione peroxidase activity, and (d) is the superoxide dismutase activity.

[0027] Figure 10 This is an analysis diagram of the effect of the intestinal physical rodent control agent provided by the present invention on the antioxidant index of mouse ileum, where (a) is the total antioxidant capacity, (b) is the reduced glutathione level, (c) is the glutathione peroxidase activity, and (d) is the superoxide dismutase activity.

[0028] Figure 11 This is an analysis diagram of the effect of the intestinal physical rodent control agent provided by the present invention on the antioxidant index of mouse colon, where (a) is the total antioxidant capacity, (b) is the reduced glutathione level, (c) is the glutathione peroxidase activity, and (d) is the superoxide dismutase activity.

[0029] Figure 12 This is an analysis diagram of the effects of the intestinal physical rodent control agent provided by the present invention on the intestinal microbial community of mice, wherein (a) is a species annotation statistical diagram, (b) is an Alpha diversity comparison analysis diagram, (c) is a Venn diagram of core species in total level grouping, (d) is a PCA analysis diagram, and (e) is a comparison diagram of differences in relative abundance of species between groups. Detailed Implementation

[0030] Embodiments of the present invention are described in detail below, examples of which are illustrated in the accompanying drawings, wherein the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary and intended to explain the present invention, and should not be construed as limiting the present invention. Example

[0031] I. Material Preparation The sodium polyacrylate used in this experiment had polymerization amounts of 300W, 1200W, 2000W, 2800W, and 8000W. Different polymerization amounts resulted in differences in water absorption, swelling, and flocculation. The aim of comparison was to simulate the pH environment of the digestive tract and select the test material with the best performance. Figure 2 As shown (where A is 300W, B is 1200W, C is 2000W, D is 2800W, and E is 8000W), tests showed that 8000W absorbs water extremely quickly, with a water absorption capacity of approximately 40 times its weight. Therefore, in this embodiment, sodium polyacrylate with a molecular weight of 8000W is preferred.

[0032] The base powder of the palatability-enhancing base is made of corn flour, roasted peanut flour, soybean meal and potato starch in a weight ratio of 3:3:2:2. Yeast extract powder of 1% and white sugar of 6% of the total weight of the base powder are added as additives.

[0033] II. Pharmaceutical Preparation Combination Figure 1 The process flow diagram shown illustrates the preparation of the intestinal physical rodent control agent in this embodiment according to the following steps: S1. Preparation of palatability base: Mix corn flour, roasted peanut flour, soybean meal and potato starch in a weight ratio of 3:3:2:2, add 1% yeast extract and 6% white sugar of the total weight of the base powder, stir well to obtain the mixed base.

[0034] S2. Add active ingredients: Add sodium polyacrylate with a molecular weight of 8000W to the mixed base material at a concentration of 10% of the total weight of the base powder, mix evenly to obtain the mixed base material.

[0035] S3. Extrusion granulation: The mixture is fed into a feed pellet mill and extruded into pelleted feed.

[0036] S4. Drying and sealing: After the granular bait is naturally air-dried, it is sealed and stored to obtain the intestinal physical rodent control agent.

[0037] The forming effect of the prepared bait pellets is shown in the figure. Figure 3 (a). Example

[0038] The only difference between this embodiment and Embodiment 1 is that the concentration of sodium polyacrylate added in step two is 20% of the total weight of the base powder.

[0039] The forming effect of the prepared bait pellets is shown in the figure. Figure 3 (b).

[0040] Comparative Example 1 The only difference between this comparative example and Example 1 is that sodium polyacrylate is not added; instead, the control feed is prepared by mixing the base powder and additives in the same proportion, extruding and granulating, and then air-drying.

[0041] Test case 1. Experimental Design To evaluate the biosafety and impact on the intestinal barrier of the intestinal physical control rodent agent prepared by this method, 24 Kunming mice (half male and half female, with similar weight) were randomly divided into 3 groups of 8 mice each (4 females and 4 males): Control group (CK group): fed with a basic diet without sodium polyacrylate (i.e., the diet prepared in Comparative Example 1). Group A (10% treatment group): fed with the feed containing 10% sodium polyacrylate prepared in Example 1 (i.e. the feed prepared in Example 1). Group B (20% treatment group): fed with the feed containing 20% ​​sodium polyacrylate prepared in Example 2 (i.e. the feed prepared in Example 2).

[0042] Mice in each group were allowed free access to food for 5 consecutive days, with daily weight and food intake recorded. After the experiment, the mice were euthanized, and serum, heart, liver, spleen, lungs, kidneys, and other organs were collected. Organ weight and colon length were recorded. Samples from the liver, ileum, and colon were preserved in liquid nitrogen, while samples from the kidneys and colon were fixed in paraformaldehyde for pathological section preparation.

[0043] 2. Feed intake measurement During the experiment, the amount of uneaten food in each group of mice was weighed daily, and the daily food intake was calculated. The results are as follows: Figure 4 As shown.

[0044] The results showed that the intestinal physical control rodent agent prepared by this method caused changes in the daily food intake of mice on day 3 within 120 hours of exposure, exhibiting a single-hump pattern of initial increase followed by decrease, with an overall decrease in food intake. One-way ANOVA showed highly significant differences in food intake among groups A, B, and CK (P<0.01). Tukey HSD post-hoc multiple comparisons showed that the food intake of groups B and CK was significantly higher than that of group A (P<0.05), while there was no statistically significant difference between groups B and CK (P>0.05).

[0045] 3. Measurement of weight change The weight of mice in each group was measured daily during the experiment, and the weight change was calculated. The results are as follows: Figure 5 As shown.

[0046] The results showed that the intestinal physical rodenticide prepared by this method caused changes in mouse body weight on day 2 within 120 hours of exposure. The control group showed little difference in body weight, while groups A and B experienced a decrease in body weight. One-way ANOVA indicated highly significant differences in the effects of different treatment groups on mouse body weight (P<0.01). The control group (CK) had the highest mean body weight, while group B had the lowest. Tukey HSD post-hoc test showed that the body weight of mice in groups A and B was significantly lower than that in the CK group (P<0.01), while there was no significant difference between groups A and B (P>0.05).

[0047] 4. Organ index measurement After the experiment, the heart, liver, spleen, lungs, kidneys, and other organs were separated and weighed, and the organ index was calculated (organ weight / body weight × 100%). The results are as follows: Figure 6 As shown.

[0048] The results of one-way analysis of variance show that there is no significant difference in the organ indices of heart and lung between groups (P>0.05), while the differences in liver, kidney and spleen indices between groups are significant (P<0.05). The results of multiple comparisons by Tukey HSD post-hoc test show that after exposure to the intestinal physical rodent control agent prepared by the present preparation method, the liver index is significantly reduced (P<0.05), showing a significant decreasing trend; the spleen and kidney indices are significantly reduced compared with the CK group (P<0.01), while there is no significant difference between test group A and test group B (P>0.05).

[0049] 5. Histopathological observation The liver, kidney and colon tissues of mice are fixed, rinsed with running water, then subjected to gradient dehydration with ethanol in increasing concentrations (75%→100%), followed by xylene clearing, wax impregnation and embedding, sectioning, section spreading and section baking to prepare wax blocks. The HE staining procedure is as follows: dewaxing with xylene, rehydration with gradient ethanol, hematoxylin staining for 10 min, differentiation with 1% hydrochloric acid ethanol for 7 s to make the nuclei appear bluish-purple; eosin staining for 10 min to make the cytoplasm and matrix appear pink; subsequent dehydration with gradient ethanol, clearing with xylene, and mounting with neutral balsam. The tissue morphology is observed under an optical microscope. The results are shown in Figure 7 .

[0050] The observation of tissue sections shows that the characteristic structures such as intestinal villi, epithelial cells, intestinal glands and smooth muscle in the CK group are morphologically normal. In the test groups (group A and group B), inflammatory cell infiltration in the ileum and colon ( Figure 7 indicated by black arrows) and muscular layer edema ( Figure 7 indicated by green arrows) are visible; inflammatory cell infiltration and cell edema are observed in the kidney. It indicates that the intestinal physical rodent control agent prepared by the present preparation method exerts a physical stimulus on the intestinal tissue after absorbing water and expanding in the digestive tract, resulting in local inflammatory reaction and tissue edema, and this change is consistent with the physical expansion mechanism of sodium polyacrylate.

[0051] 6. Colon length measurement After the test, the colon of mice in each group is isolated, and the colon length is measured. The results are shown in Figure 8 .

[0052] The results of descriptive statistics show that group B has the longest colon length, and the control group CK has the shortest colon length. The results of one-way analysis of variance show that there is no significant difference in the mean colon length among the three groups (P>0.05). Tukey HSD post-hoc test shows that the difference between groups does not reach a statistically significant level (P>0.05), but the colon length among the three groups shows a gradually increasing trend: CK group < group A < group B. This trend suggests that after sodium polyacrylate absorbs water and expands in the intestinal tract, it may exert a physical pulling effect on the intestinal tract, leading to a dose-dependent increasing trend of colon length.

[0053] 7. Detection of serological indicators In this test, 12 serological indicators were detected, namely IgG (Immunoglobulin G), IgM (Immunoglobulin M), UREA (Urea), TC (Total Cholesterol), TG (Triglyceride), ALT (Alanine Aminotransferase), AST (Aspartate Aminotransferase), TP (Total Protein), ALB (Albumin), GLOB (Globulin), A / G (Albumin / Globulin Ratio), and ALP (Alkaline Phosphatase). The detection method was performed with reference to the instructions of a conventional kit. The results are shown in Table 1.

[0054] Table 1. Detection results of serological indicators in mice exposed to the intestinal physical rodent control agent prepared by the present preparation method

[0055] Note: Different lowercase letters in the same row indicate significant differences between groups (P<0.05).

[0056] The serological detection results show that: there are no significant differences in the levels of IgG, IgM and TC between groups (P>0.05); there are extremely significant differences in the levels of UREA and TG between groups (P<0.001), wherein UREA increases in a concentration-dependent manner (CK group < A group < B group), and TG decreases in a concentration-dependent manner; there are significant or extremely significant differences in ALT, AST, TP, ALB, GLOB and A / G between groups (P<0.05~0.01), wherein ALT and AST increase with the increase of sodium polyacrylate concentration, and TP and GLOB decrease with the increase of concentration; the difference in ALP between groups does not reach a statistically significant level (P>0.05).

[0057] The above results indicate that the intestinal physical rodent control agent prepared by the present preparation method has a certain effect on the liver and kidney functions of mice, suggesting that sodium polyacrylate, after absorbing water and swelling in the digestive tract, may exert a systematic effect on the metabolism of the organism, which is consistent with its mechanism of physical rodent control.

[0058] 8. Detection of Antioxidant Indicators The activities of total antioxidant capacity (T-AOC), reduced glutathione (GSH), glutathione peroxidase (GPX) and superoxide dismutase (SOD) in the liver, ileum and colon tissues of mice were detected respectively. The detection method was performed with reference to the kit instructions of Beijing Solarbio Science & Technology Co., Ltd. The results are shown in Figure 9 (Liver), Figure 10 (Ileum) and Figure 11 (Colon).

[0059] 8.1 Liver Antioxidant Indicators One-way ANOVA results for liver T-AOC showed that there were highly significant differences in the effects of different treatment groups on liver T-AOC levels in mice (P<0.001). The liver T-AOC level in group B was significantly higher than that in groups CK and A; the levels in groups CK and A were similar and both were at low levels. Tukey HSD post-hoc test showed highly significant differences between groups B and CK, and between groups B and A (P<0.001), while there was no significant difference between groups CK and A (P>0.05).

[0060] One-way ANOVA of liver GSH levels showed that there were significant differences in the effects of different treatment groups on liver GSH activity in mice (P<0.01). Group A mice had the highest liver GSH levels, which were significantly higher than those in Group B and the CK group. Tukey HSD post-hoc test showed that there were significant differences between Group A and the CK group, and between Group A and Group B (P<0.05), while there was no significant difference between the CK group and Group B (P>0.05).

[0061] One-way ANOVA of liver GPX activity showed that different treatment groups had significant differences in the effect of GPX activity on mouse liver (P<0.05). The mean GPX activity in the liver of mice in the CK group was the lowest, the mean GPX activity in the liver of mice in the B group was only higher than that in the CK group, while the GPX content in the A group was lower than that in the B group but the GPX activity was the highest. Tukey HSD post-hoc test results showed that the liver GPX activity in the A group was significantly higher than that in the CK group (P<0.001), and there were no significant differences among the other groups.

[0062] One-way ANOVA of liver SOD activity showed that different treatment groups had significant differences in the effect of liver SOD activity on mice (P<0.01). The CK group had the highest liver SOD activity, the B group had lower liver SOD activity than the CK group, and the A group had the lowest SOD activity. Tukey HSD post-hoc test results showed that there was a significant difference between the A and CK groups (P<0.01), while there were no significant differences among the other groups (P>0.05).

[0063] 8.2 Ileal antioxidant indices One-way ANOVA of ileal T-AOC levels showed that there was no significant difference in the effect of different treatment groups on ileal T-AOC levels in mice (P>0.05). Group B mice had the highest ileal T-AOC levels, followed by Group A, and the CK group had the lowest levels.

[0064] One-way ANOVA of ileal GSH activity showed that there were highly significant differences in the effects of different treatment groups on ileal GSH levels in mice (P<0.001). Group A mice had the highest ileal GSH levels, followed by Group B, and both groups were significantly higher than the CK group. Tukey HSD post-hoc test showed highly significant differences between the CK group and Group A, and between the CK group and Group B (P<0.001), while there was no significant difference between Group A and Group B (P>0.05).

[0065] One-way ANOVA of ileal GPX activity showed that there were highly significant differences in the effects of different treatment groups on ileal GPX activity (P<0.001). Group B mice had the highest ileal GPX activity, followed by the CK group, and the lowest in group A. Tukey HSD post-hoc test results showed that the ileal GPX activity in group B was significantly higher than that in the CK and A groups (all P<0.001), while there was no significant difference between group A and the CK group (P>0.05).

[0066] One-way ANOVA results showed that there was no significant difference in the effect of different treatment groups on the ileum SOD activity of mice (P>0.05). The CK group had the lowest ileum SOD activity, the B group had the highest, and the A group was in between.

[0067] 8.3 Colonic antioxidant markers One-way ANOVA of colonic T-AOC levels showed no statistically significant difference in T-AOC levels among different treatment groups (P>0.05). Group A mice had the highest T-AOC levels, followed by Group B, and the CK group had the lowest. Tukey HSD post-hoc test results showed a significant difference between Group A and CK (P<0.05), while no statistically significant differences were found between the other groups.

[0068] One-way ANOVA of colonic GSH activity showed highly significant differences in the effects of different treatment groups on colonic GSH activity in mice (P<0.001). Group A mice had the highest colonic GSH level, significantly higher than the CK and B groups; the level in group B was slightly higher than that in the CK group. Tukey HSD post-hoc test showed highly significant differences between group A and CK, and between group A and B (P<0.001), while there was no significant difference between group CK and group B (P>0.05).

[0069] One-way ANOVA of colonic GPX activity showed that different treatment groups had significant differences in the effect of colonic GPX activity in mice (P<0.01). The mean colonic GPX activity in group B was the lowest, while the GPX activity in group A was higher than that in group B but lower than that in group CK. Tukey HSD post-hoc test results showed that the colonic GPX activity in groups A and B was significantly lower than that in group CK (both P<0.01), and there was no significant difference between groups A and B (P>0.05). The colonic GPX activity among the three groups showed a decreasing trend in order: CK group > group A > group B.

[0070] One-way ANOVA of colonic SOD activity showed that there was no significant difference in the effect of different treatment groups on colonic SOD activity in mice (P>0.05). Group B mice had the highest colonic SOD activity, followed by CK group, and group A had the lowest.

[0071] The above antioxidant index results indicate that the intestinal physical rodent control agent prepared by this method has a differentiated effect on the antioxidant system of different tissues (liver, ileum, colon). This may be related to the different degrees of water absorption and swelling of sodium polyacrylate in different intestinal segments, suggesting that the agent triggers the body's oxidative stress response through physical action in the intestine.

[0072] 9. Intestinal Microbiome Analysis Mouse fecal samples (0.2g) were collected, and total microbial DNA was extracted. After quality control, metagenomic libraries were constructed and sequenced using the Illumina NovaSeq 6000 platform. α-diversity was assessed using Shannon and Simpson indices to evaluate species richness and evenness; β-diversity was based on Bray-Curtis and Unifrac distances, and differences in community structure between groups were tested using PCoA and PERMANOVA. Species and functional differences were analyzed using ANOVA and LEfSe, combined with KEGG functional pathway analysis, to screen for differentially expressed species and biomarkers between groups. Statistical analysis was performed using R language, and data are expressed as mean ± standard deviation. Results are as follows: Figure 12 As shown.

[0073] Species richness analysis showed that, compared with the control group, the species richness of the gut microbiota in groups A and B mice changed significantly, with the abundance of some dominant bacterial groups increasing and the abundance of rare bacterial groups decreasing. The change in species richness in group B was greater than that in group A, showing a certain concentration dependence. Alpha diversity comparison analysis, Venn diagram of core species, PCA analysis, and comparison of differences in relative species richness between groups all showed that sodium polyacrylate exposure can significantly alter the gut microbiota structure in mice.

[0074] The above results indicate that the intestinal physical rodent control agent prepared by this method not only has a direct mechanical effect on the intestine through physical expansion, but may also affect the composition and abundance of intestinal microorganisms by changing the intestinal microenvironment. Industrial applicability

[0075] The preparation method and application of the intestinal physical rodent control agent provided by this invention have the following industrial applicability: (1) The intestinal physical rodent control agent prepared by this preparation method is based on sodium polyacrylate, which has a wide range of raw material sources, low cost, and is suitable for large-scale industrial production.

[0076] (2) The preparation method of the present invention can be completed by conventional feed pellet mill, without special equipment, with simple process flow, convenient operation and good industrial scalability.

[0077] (3) The intestinal physical rodent control agent prepared by this preparation method is a physical rodent control mechanism, which does not involve chemical poisoning components. It is environmentally friendly, conforms to the development direction of green prevention and control, and can be widely used in the field of rodent control in grasslands, farmland, forest land and other fields.

[0078] The above description discloses only one preferred embodiment of the present invention, and should not be construed as limiting the scope of the present invention. Those skilled in the art will understand that all or part of the processes of the above embodiments can be implemented, and equivalent changes made in accordance with the claims of the present invention are still within the scope of the invention.

Claims

1. A method for preparing an intestinal physical rodent control agent, characterized in that, Includes the following steps: Prepare the palatability-enhancing base material: Mix the base powder and additives evenly according to the preset ratio to obtain the mixed base material; Adding active ingredients: Add sodium polyacrylate to the mixed base material and mix evenly to obtain a mixed material; Extrusion granulation: The mixture is fed into a feed pellet mill and extruded into pelleted feed. Drying and sealing: After the granular bait is air-dried naturally, it is sealed and stored to obtain the intestinal physical rodent control agent.

2. The method for preparing the intestinal physical rodent control agent as described in claim 1, characterized in that, In the step of preparing the palatability-enhancing base material, the base powder consists of corn flour, roasted peanut powder, soybean meal, and potato starch.

3. The method for preparing the intestinal physical rodent control agent as described in claim 2, characterized in that, In the step of preparing the palatability-enhancing base material, the ratio of corn flour, cooked peanut flour, soybean meal and potato starch is 3:3:2:

2.

4. The preparation method and application of the intestinal physical rodent control agent as described in claim 3, characterized in that, In the step of preparing the palatability-enhancing base material, the additives include yeast extract and white sugar. The amount of yeast extract added is 1% of the total weight of the base powder, and the amount of white sugar added is 6% of the total weight of the base powder.

5. The method for preparing the intestinal physical rodent control agent as described in claim 4, characterized in that, In the step of adding active ingredients, the molecular weight of the high molecular weight sodium polyacrylate is 8000W.

6. The method for preparing the intestinal physical rodent control agent as described in claim 5, characterized in that, In the step of adding active ingredients, the concentration of the added sodium polyacrylate is 10% to 20% of the total weight of the base powder.

7. The application of an intestinal physical rodent control agent prepared by the preparation method of any one of claims 1 to 6 in the prevention and control of rodent pests.

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