A liquor with the function of relieving fatty liver and a preparation method and application thereof

Abstract

The present application belongs to the field of functional food and food technology, and particularly relates to a liquor with the function of relieving fatty liver and a preparation method and application thereof. To provide a liquor which can be verified in a real base liquor environment, has clear proportioning, coordinated flavor and the function of relieving fatty liver caused by long-term chronic drinking, the liquor is composed of a light-flavor liquor base, fructo-oligosaccharide, plant extract and water for blending; wherein the content of fructo-oligosaccharide is 50-120 g / L, and the volume fraction of the plant extract is 1%-10%. The plant extract is obtained by cold extraction of plant raw materials with the light-flavor liquor base, and the plant raw materials include lophatherum gracile, gardenia, chrysanthemum, costus, clove, dried tangerine or orange peel, kaempferia galanga, cardamom, angelica, rhynchosia volubilis, zanthoxylum, sandalwood.

Description

Technical Field

[0001] This invention belongs to the field of functional foods and food technology, specifically relating to a liqueur with the function of alleviating fatty liver formation, its preparation method, and its application. Background Technology

[0002] Liqueurs are an important category of traditional Chinese alcoholic beverages, typically using baijiu (Chinese white liquor) as a base, combined with plant extracts and sugar sources to create a specific flavor. Long-term or excessive alcohol consumption may lead to changes such as lipid accumulation in the liver, fatty degeneration, and oxidative stress. Existing related products often exhibit the following:

[0003] (1) Insufficient efficacy verification: Most products are based on empirical compatibility and lack systematic evaluation in real complex wine matrices;

[0004] (2) Insufficient optimization of functional group allocation ratio: The amount of fructooligosaccharides and plant extracts added is mostly adjusted based on flavor, lacking dose-response relationship;

[0005] (3) Limitations of the evaluation model: Conventional evaluations often use pure ethanol to create models, which makes it difficult to reflect the comprehensive impact under the real base wine matrix.

[0006] Therefore, there is an urgent need to provide a liqueur that can be verified in a real base wine environment, has a clear formula, a harmonious flavor, and can alleviate the tendency of fatty liver caused by long-term chronic drinking. Summary of the Invention

[0007] This invention addresses the aforementioned problems by providing a liqueur with the function of alleviating fatty liver formation, along with its preparation method and application. By performing gradient screening of functional components on a real base liquor matrix, a more reproducible optimal ratio is determined, and a stable process suitable for industrialization is provided.

[0008] To achieve the above objectives, the present invention employs the following technical solution:

[0009] This invention provides a liquor with the function of alleviating fatty liver formation, composed of light-aroma baijiu base liquor, fructooligosaccharides, plant extracts, and blending water; the content of fructooligosaccharides in the liquor is 50~120 g / L; the volume fraction of the plant extract in the liquor is 1%~10%; the plant extract is obtained by extracting plant raw materials from light-aroma baijiu base liquor at a mass-volume ratio of 1:5 to 1:50, and the plant raw materials include light bamboo leaves, gardenia, chrysanthemum, costus root, clove, dried tangerine peel, galangal, amomum villosum, angelica sinensis, fragrant grass, tonka bean, and sandalwood.

[0010] Furthermore, the content of the oligofructose in the liqueur is 95 g / L; the volume fraction of the plant extract in the liqueur is 5%.

[0011] Furthermore, the light-aroma baijiu base liquor is aged light-aroma baijiu with an alcohol content ranging from 20% to 70% vol, preferably aged Fenjiu; the blending water is Xinghuacun deep karst weakly alkaline mineral water or weakly alkaline drinking water with a mineralization / alkalinity equivalent to that of the base liquor.

[0012] Furthermore, the plant materials are pre-treated by being washed, drained, dried or air-dried, and then chopped / pulverized to 10-40 mesh.

[0013] This invention also provides a method for preparing a liqueur with the function of alleviating fatty liver formation, comprising the following steps:

[0014] (1) After pretreatment of the plant raw materials, place them in a container and add light-aroma baijiu base liquor at a ratio of 1:5 to 1:50 (mass-volume ratio) between the plant raw materials and light-aroma baijiu base liquor. Cold soak and extract at room temperature for 15 to 25 days, stirring or circulating during the process. After extraction, filter and purify to obtain plant extract.

[0015] (2) Add oligofructose to the light aroma type baijiu base liquor and dissolve it completely. Then add the plant extract obtained in step (1) and the blending water, and stir or circulate to make the liquor uniform.

[0016] (3) The blended liquor is aged for 3 to 6 months, and then filtered to obtain the finished liqueur.

[0017] Furthermore, the filtration in step (1) is 200-500 mesh filtration or equivalent fine filtration; the purification includes one or more of the following: settling, centrifugation, plate and frame filtration or membrane filtration.

[0018] This invention also provides an application of a liqueur that can alleviate fatty liver formation, in the preparation of food or beverages that can improve liver lipid deposition and fatty liver function caused by long-term chronic drinking, without involving the prevention or treatment of disease.

[0019] This invention also provides a method for screening / evaluating liqueur formulations. Using light-aroma baijiu base liquor as a modeling matrix, a liver lipid deposition evaluation system under base liquor exposure conditions is constructed. In this system, the addition amounts of fructooligosaccharides and plant extracts are sequentially set and compared in a gradient manner to screen and obtain an optimal ratio that reduces liver lipid deposition. The preferred ratio is 95 g / L of fructooligosaccharides and 5% of plant extracts.

[0020] Compared with the prior art, the present invention has the following advantages:

[0021] 1) Clear formulation and stronger repeatability: Gradient screening was completed and the optimal formulation (95 g / L fructooligosaccharides + 5% plant extract) was determined under the real base wine matrix.

[0022] 2) The evaluation approach is more application-oriented: using base wine as the modeling matrix is ​​closer to the actual drinking scenario;

[0023] 3) Stable process: The combination of room temperature cold maceration and aging filtration facilitates large-scale production, resulting in a harmonious flavor and good stability;

[0024] 4) Clearer food attribute boundaries: The term "relieve fatty liver" in this invention is used to describe the functional tendency of improving liver lipid metabolism and reducing lipid deposition, and is not used for disease prevention or treatment. Attached Figure Description

[0025] Figure 1 This is a schematic diagram illustrating the effects of different treatment groups on liver lipid deposition and their quantitative analysis during the screening stage of fructooligosaccharide concentration. In this diagram, A is an image of oil red O staining of zebrafish liver, scale bar: 20 μm, 400×; B is the quantitative analysis of oil red O staining, where FOS represents fructooligosaccharides, *P<0.05, **P<0.01, ***P<0.001 (n=3).

[0026] Figure 2 This is a schematic diagram showing the comparison of liver tissue pathology among different treatment groups during the screening stage of fructooligosaccharide concentration.

[0027] Figure 3 This is a schematic diagram illustrating the effects of different treatment groups on liver lipid deposition and their quantitative analysis during the plant extract concentration screening stage. In this diagram, A is an image of zebrafish liver stained with Oil Red O, scale bar: 20 μm, 400×; B is the quantitative analysis of Oil Red O staining, where FOS represents fructooligosaccharides, *P<0.05, ***P<0.001 (n=3).

[0028] Figure 4 This is a schematic diagram showing the comparison of liver tissue pathology among different treatment groups during the plant extract concentration screening stage.

[0029] Figure 5 This diagram illustrates the effects of each treatment group on hepatic lipid deposition and their quantitative analysis during the validation phase. A shows the Oil Red O staining image of zebrafish liver (scale bar: 20 μm, 400×); B shows the quantitative analysis of Oil Red O staining, where FOS represents fructooligosaccharides. ***P < 0.001 (n = 3).

[0030] Figure 6 This is a schematic diagram showing the comparison of liver tissue pathology among different treatment groups during the validation phase. Detailed Implementation

[0031] To further illustrate the technical solution of the present invention, the present invention will be further described below through embodiments.

[0032] Example 1: Preparation of Liqueur

[0033] A method for preparing a liqueur with the function of alleviating fatty liver caused by long-term chronic alcohol consumption includes the following steps:

[0034] 1) Preparation of plant extract: Weigh 10 parts of Lophatherum gracile, 10 parts of Gardenia jasminoides, 10 parts of Chrysanthemum morifolium, 10 parts of Aucklandia lappa, 10 parts of Clove, 10 parts of Tangerine peel, 10 parts of Kaempferia galanga, 10 parts of Amomum villosum, 10 parts of Angelica sinensis, 10 parts of Ligusticum striatum, 10 parts of Aristolochia debilis, and 10 parts of Sandalwood. After washing, draining, drying or air-drying, cut / crush to 10-40 mesh for pretreatment and place in a ceramic jar. Add light-aroma baijiu base liquor at a ratio of 1:5 to 1:50 (mass-volume ratio). Cold soak extraction at room temperature for 21 days, stirring or circulating twice a day during the period. After extraction, filter and purify to obtain plant extract.

[0035] 2) Blending: Add fructooligosaccharides with a purity of ≥95% to a fixed amount of high-quality aged light-aroma Fenjiu base liquor to make a final concentration of 95 g / L. After fully dissolving, add the above-mentioned plant extract to make a volume fraction of 5%, and then add an appropriate amount of Xinghuacun deep karst weakly alkaline mineral water for blending. Stir or circulate with an air pump until the liquor is uniform.

[0036] 3) Aging and Filtration: After aging the blended liquor for 3 to 6 months, filter it until it is transparent and clear to obtain the finished liqueur.

[0037] Example 2: Functional Component Concentration Screening (Gradient Screening Based on Real Base Wine Matrix)

[0038] A method for screening / evaluating liqueur formulations involves using light-aroma baijiu base liquor as a modeling matrix to construct an evaluation system for liver lipid deposition under base liquor exposure conditions. In this system, the addition amounts of fructooligosaccharides and plant extracts are sequentially set and compared in a gradient manner to screen and obtain the optimal ratio that reduces liver lipid deposition.

[0039] Phase 1: Screening using fructooligosaccharide concentration gradients:

[0040] 1) Group design (5 groups in total):

[0041] (1) Blank control group: water used for systemic aquaculture;

[0042] (2) Ethanol control group: a pure ethanol solution with the same ethanol concentration as the base wine;

[0043] (3) Base liquor model group: high-quality aged Fenjiu base liquor (concentration of LC) 50 (1 / 25 of)

[0044] (4) Low-dose fructooligosaccharide group: base liquor + 50 g / L fructooligosaccharide;

[0045] (5) High dose of fructooligosaccharide group: base wine + 95 g / L fructooligosaccharide.

[0046] 2) Experimental Methods: Healthy adult zebrafish were grouped and exposed to the environment for four consecutive weeks, with the exposure solution changed daily. After the exposure, livers were harvested for Oil Red O staining and H&E staining. LC 50 The determination can be obtained using the acute exposure method (e.g., setting multiple concentration gradients, recording mortality rates within a certain time window, and using regression to obtain the LC). 50 ), then convert to get 1 / 25 LC 50 The corresponding amount of base liquor added.

[0047] 3) Results ( Figure 1 and Figure 2 Oil Red O staining and quantitative analysis showed that lipid accumulation in the high-dose group (base wine + 95 g / L fructooligosaccharide) was decreasing compared to the low-dose group (base wine + 50 g / L fructooligosaccharide), but there was no significant difference.

[0048] 4) Conclusion: 95 g / L of fructooligosaccharide was selected as the fixed base concentration for subsequent compound formulation experiments.

[0049] Phase Two: Plant Extract Concentration Gradient Screening

[0050] Using the same base wine matrix conditions, the concentration of plant extracts was screened (with a fixed fructooligosaccharide concentration of 95 g / L).

[0051] 1) Group design (6 groups in total):

[0052] (1) Blank control group: water used for systemic aquaculture;

[0053] (2) Ethanol control group: a pure ethanol solution with the same ethanol concentration as the base wine;

[0054] (3) Base liquor model group: high-quality aged Fenjiu base liquor (concentration of LC) 50 (1 / 25 of)

[0055] (4) Combined intervention group A: base wine + 95 g / L fructooligosaccharides + 1% plant extract;

[0056] (5) Combined intervention group B: base wine + 95 g / L fructooligosaccharides + 5% plant extract;

[0057] (6) Combined intervention group C: base wine + 95 g / L fructooligosaccharides + 10% plant extract.

[0058] 2) Experimental method: Same as stage one.

[0059] 3) Results ( Figure 3 and Figure 4Oil Red O staining and quantitative analysis showed that the lipid droplet area ratio in the combined intervention group B (5% extract) was the lowest, significantly lower than that in the model group and other combined groups (P<0.001).

[0060] 4) Conclusion: 95 g / L fructooligosaccharide + 5% plant extract is the optimal synergistic ratio.

[0061] Example 3: Independent Validation of the Preferred Formulation

[0062] 1) Group design (4 groups in total): blank control group, ethanol control group, base wine model group (1 / 25 LC) 50 ), combined intervention group (base wine + 95 g / L fructooligosaccharides + 5% plant extract).

[0063] 2) Experimental method: Same as Example 2.

[0064] 3) Results ( Figure 5 and Figure 6 The optimized formulation group showed a significant reduction in lipid droplet accumulation (P<0.001) and a marked improvement in liver tissue structure, further validating the stability of the optimized formulation.

[0065] Example 4: Stability Study of Finished Product

[0066] The selected formula liqueur showed no sedimentation or obvious stratification under conditions of 12 months of storage at room temperature and 6 months of accelerated testing at 37 ℃, with stable physicochemical indicators and harmonious flavor.

[0067] Without changing cold maceration as the core extraction method, ultrasonic / microwave methods can be used as auxiliary means to shorten extraction time or increase the yield of specific components, depending on industrial scale-up and flavor requirements. The corresponding process parameters should be determined and recorded according to equipment specifications and target component characteristics to ensure repeatable production.

[0068] The foregoing has shown and described the main features and advantages of the present invention. It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the invention can be implemented in other specific forms without departing from its spirit or essential characteristics. Therefore, the embodiments should be considered exemplary and non-limiting in all respects, and the scope of the invention is defined by the appended claims rather than the foregoing description. Thus, it is intended that all variations falling within the meaning and scope of equivalents of the claims be included within the present invention.

[0069] Furthermore, it should be understood that although this specification describes embodiments, not every embodiment contains only one independent technical solution. This narrative style is merely for clarity. Those skilled in the art should consider the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

Claims

1. A type of liqueur with the function of alleviating fatty liver formation, characterized in that, It is composed of light-aroma baijiu base liquor with an alcohol content of 20-70% vol, fructooligosaccharides, plant extracts, and water for blending; the content of fructooligosaccharides in the liquor is 50-120 g / L; the volume fraction of the plant extract in the liquor is 1%-10%; the plant extract is obtained by extracting plant raw materials from light-aroma baijiu base liquor, and the plant raw materials include light bamboo leaves, gardenia, chrysanthemum, costus root, clove, dried tangerine peel, galangal, amomum villosum, angelica sinensis, fragrant grass, tonka bean, and sandalwood.

2. The liqueur with the function of alleviating fatty liver formation according to claim 1, characterized in that, The content of the oligofructose in the liqueur is 95 g / L; the volume fraction of the plant extract in the liqueur is 5%.

3. The liqueur with the function of alleviating fatty liver formation according to claim 2, characterized in that, The base liquor for the light-aroma baijiu is aged light-aroma baijiu; the blending water is weakly alkaline mineral water from the deep karst springs of Xinghuacun or weakly alkaline drinking water with a similar mineralization / alkalinity.

4. A liqueur with the function of alleviating fatty liver formation according to claim 3, characterized in that, The plant materials are pre-treated by washing, draining, drying or air-drying, and then cutting / crushing to 10-40 mesh.

5. A method for preparing a liqueur with the function of alleviating fatty liver formation as described in any one of claims 1 to 4, characterized in that, Includes the following steps: (1) After pretreatment of the plant raw materials, place them in a container and add light-aroma baijiu base liquor at a mass-volume ratio of 1:5 to 1:

50. Soak and extract at room temperature for 15 to 25 days, stirring or circulating during the process. After extraction, filter and purify to obtain plant extract. (2) Add oligofructose to the light aroma type baijiu base liquor and dissolve it completely. Then add the plant extract obtained in step (1) and the blending water, and stir or circulate to make the liquor uniform. (3) The blended liquor is aged for 3 to 6 months, and then filtered to obtain the finished liqueur.

6. The method for preparing a liqueur with the function of alleviating fatty liver formation according to claim 5, characterized in that, The filtration in step (1) is 200-500 mesh filtration or equivalent fine filtration; the purification includes one or more of the following: settling, centrifugation, plate and frame filtration or membrane filtration.

7. The application of the liqueur with the function of alleviating fatty liver formation as described in any one of claims 1 to 4, characterized in that, Application in the preparation of foods or beverages that can improve liver lipid deposition and fatty liver function caused by long-term chronic alcohol consumption.

8. A method for screening / evaluating liqueur formulations, characterized in that, Using light-aroma baijiu base liquor as the modeling matrix, an evaluation system for liver lipid deposition under base liquor exposure conditions was constructed. In this system, the addition amounts of fructooligosaccharides and plant extracts were sequentially set and compared to screen out the optimal ratio that reduces liver lipid deposition.

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