A bacteria liquid separation device for fecal bacteria transplantation

Abstract

The application discloses a kind of bacteria liquid separation devices of fecal bacteria transplantation, belong to medical instrument field.The device includes airtight casing, sealing cover, sample processing system, pneumatic stirring system, multistage gradient filtration system, bacteria liquid collection system, temperature control system, gas control system and control system.Sample processing system contains sample container, diluent injection unit and homogeneous unit.Pneumatic stirring system is formed into spiral flow by gas injection nozzle to sample container inside tangential injection gas, realizes the mixing stirring of no mechanical contact.Multistage gradient filtration system is made of at least three filtering units with gradually reduced aperture, and the aperture of the last stage is 10-30 μm, which is combined with negative pressure driving to realize high-efficiency fine filtration.Temperature control system maintains low-temperature environment of 2-8℃, and gas control system controls the oxygen concentration below 1%, to ensure the activity of strict anaerobic bacteria.The device solves the problems of existing equipment, such as mechanical stirring damaging bacterial population, difficulty in balancing filtration precision and efficiency, lack of whole-process low-temperature anaerobic protection, and is suitable for standardized bacteria liquid preparation of clinical fecal bacteria transplantation.

Description

Technical Field

[0001] This invention relates to the field of medical device technology, specifically to a bacterial culture separation device for fecal microbiota transplantation. More specifically, it relates to an automated separation device capable of efficiently extracting highly active intestinal flora from fecal samples under fully enclosed conditions through a combination of gas-driven stirring and multi-stage gradient filtration. Background Technology

[0002] Currently, there are various devices and technical solutions available both domestically and internationally for the separation and extraction of fecal bacteria.

[0003] Patent CN213790326U discloses a fecal microbiota transplantation and separation device, which mainly consists of a sampling unit, a filtration unit, and a dispensing unit. This device purifies the bacterial solution using a circulating filtration method. However, in the disclosed technical solution, each functional unit is assembled as an independent module, resulting in a large overall space occupation for the device. More importantly, the device relies on a mechanical stirring module for mixing, and the heat generated by the motor during operation is conducted along the drive shaft to the interior of the mixture, causing the temperature of the mixture to rise. According to our preliminary experimental data, after continuous stirring for 10 minutes at room temperature (25℃), the core temperature of the mixture can rise to over 32℃. This temperature change has a significant negative impact on the activity of strictly anaerobic bacteria.

[0004] Patent CN211814386U discloses a fecal microbial extraction and separation device, which adopts a stacked structure of a liquid mixing chamber, a filtration chamber, and a bacterial liquid collection chamber connected sequentially from top to bottom by threads. The device includes a propeller-type agitator in the mixing chamber and multiple layers of screens in the filtration chamber. However, this design still relies on mechanical stirring, and the problem of motor overheating remains unresolved. Furthermore, the filtration process depends on natural sedimentation due to gravity, and the processing time is typically over 30 minutes, which further exacerbates the decline in bacterial activity.

[0005] Patent CN112742096B discloses a device for separating and extracting fecal microbiota from intestinal contents, which incorporates ultrasonic sieve technology. This device integrates multiple independent units, including an electrical control cabinet, a deodorizing tank, a cleaning mechanism, a drying mechanism, and a residue removal mechanism. While this solution improves automation, its complex structure, high manufacturing cost, and large footprint limit its widespread application in space-constrained clinical departments. Furthermore, the potential mechanical damage to the cell walls of bacterial flora caused by ultrasonic vibrations is not adequately addressed in this patent.

[0006] Regarding non-patented technologies, most reported methods for preparing bacterial cultures employ a three-step process of "stirring-filtration-centrifugation." This method first uses a magnetic stirrer or vortex mixer to mix the fecal sample with a diluent, then filters it sequentially through sieves of different pore sizes, and finally obtains bacterial precipitate through centrifugation or differential centrifugation. Each step of this process is carried out in an open environment, posing a risk of cross-contamination. Furthermore, the mechanical forces generated during centrifugation significantly impact the activity of the bacterial community. More importantly, the entire process is time-consuming (typically 1-2 hours), and the longer the bacterial community is exposed to an aerobic environment, the greater the loss of its activity.

[0007] Furthermore, existing technologies also include fecal microbial mixing devices without rotating parts. These devices utilize inert gas introduced through air inlet pipes on both sides of the bottle, relying on rising air bubbles to drive the liquid flow. While this device avoids mechanical stirring, the movement of air bubbles in the liquid is relatively disordered, making it difficult to guarantee uniform mixing. Additionally, the filter element in this device is only located at the bottle opening, employing single-stage filtration. For complex mixtures like fecal samples, single-stage filtration is prone to clogging the filter pores, and it is difficult to simultaneously meet the requirements of high throughput and high purity in terms of filtration accuracy.

[0008] In summary, existing technologies generally suffer from the following shortcomings: (1) Insufficient automation and closed-loop system. Most existing equipment is still based on conventional laboratory equipment, requiring manual completion of multiple steps such as sample transfer, stirring, and filtration. The operation process is cumbersome and difficult to standardize. During manual operation, operators will inevitably come into contact with odors and there is a risk of sample contamination.

[0009] (2) Insufficient protection of bacterial community activity. On the one hand, the temperature rise and shear force generated by mechanical stirring cause double damage to the bacterial community; on the other hand, most existing devices are not equipped with active temperature control and anaerobic maintenance systems, making it difficult to provide a suitable living environment for strict anaerobic bacteria throughout the entire process of bacterial solution preparation.

[0010] (3) It is difficult to balance efficiency and precision in filtration performance. Existing solutions using single-stage or two-stage filtration either have excessively large pore sizes that allow impurities to penetrate, or excessively small pore sizes that result in slow filtration speed and easy clogging. There is a lack of reasonable pore size gradient design and anti-clogging mechanism.

[0011] To address the shortcomings of the existing technologies, it is necessary to develop a highly integrated fecal microbial separation device that can maintain a low-temperature anaerobic environment throughout the process and has gradient filtration and self-cleaning functions. This has urgent clinical needs and practical application value. Summary of the Invention

[0012] (a) Technical problems to be solved To address the aforementioned problems in existing technologies, this invention proposes a bacterial culture separation device for fecal microbial transplantation. The core design concept of this device is to organically integrate four key technologies: pneumatic stirring, multi-stage gradient filtration, low-temperature anaerobic environment maintenance, and automated control, forming a fully enclosed bacterial culture preparation system.

[0013] (II) Technical Solution To achieve the above objectives, the present invention provides the following technical solution: A fecal microbiota transplantation (FMT) bacterial culture separation device includes a sealed housing and an openable sealing cap. The interior of the housing forms a closed containment space for accommodating the various functional components that perform sample processing, mixing, filtration, and bacterial culture collection. The sealing cap and housing are airtightly fitted together via a sealing element, thereby maintaining the isolation of the internal space from the external environment throughout the operation of the device.

[0014] Inside the casing, a sample processing system is first installed. This system includes a container for holding fecal samples, a unit for automatically injecting diluent into the container, and a unit for preliminary homogenization of the samples. The sample container has a sample inlet at the top and a discharge outlet at the bottom. The diluent injection unit is connected to the sample container via piping, and the injection volume is automatically controlled by the control system according to a preset liquid-to-solid ratio. The homogenization unit uses a stirring paddle to perform a short period of low-speed stirring after the diluent is added to the sample, so that the fecal sample is initially dispersed into a suspension.

[0015] To achieve thorough mixing of the sample and diluent without introducing a mechanical heat source, this device abandons traditional mechanical stirring and instead employs a pneumatic stirring system. This system includes a gas generator, a gas delivery pipeline, and several gas nozzles mounted on the inner wall of the sample container. The outlet direction of the gas nozzles forms a certain angle with the tangential direction of the inner wall of the sample container. When pressurized gas is ejected from the nozzles, it creates a swirling flow along the wall within the sample container, driving the liquid to produce a vortex-like flow pattern. Compared to passive mixing methods that rely on the disordered upward movement of bubbles, this tangentially ejected swirling flow has a more defined hydrodynamic path and higher mixing efficiency. Simultaneously, a pulse generator is installed in the gas delivery pipeline, causing the ejected gas flow to be intermittently pulsed, thereby generating periodic disturbances within the container and further improving the uniformity of mixing.

[0016] The thoroughly mixed fecal suspension then enters a multi-stage gradient filtration system. This system, located downstream of the sample container, consists of at least three filtration units connected in series. Each filtration unit is filled with filter media of different pore sizes, with the pore size decreasing progressively along the direction of liquid flow. The advantage of this gradient design is that the initial stage (large pore size) performs coarse filtration, trapping large particles such as food residue; the intermediate stage (medium pore size) removes medium-sized impurities; and the final stage (small pore size) performs fine filtration, ensuring that the effluent primarily contains the target bacterial flora. Through this staged interception strategy, the solid load on each stage of filter media is rationally distributed, avoiding the problem of rapid clogging that occurs when a single small-pore filter directly faces coarse impurities.

[0017] The driving force required for the filtration process is provided by the bacterial culture collection system. Located downstream of the filtration system, this collection system mainly consists of a collection container and a negative pressure generator. The negative pressure generator creates a controllable negative pressure environment within the collection container, causing the liquid in the sample container to flow sequentially through each stage of the filtration unit under the pressure difference between atmospheric pressure and the negative pressure, ultimately entering the collection container.

[0018] To ensure the activity of the microbial community throughout the treatment process, this device also integrates a temperature control system and a gas control system. The temperature control system uses a temperature sensor to monitor the internal temperature of the shell in real time and maintains it within a preset target range through cooling or heating elements, typically controlled at a low temperature of 2-8°C, to inhibit enzyme activity and microbial metabolic rate, and slow down the natural decline of microbial activity. The gas control system monitors the oxygen content inside the shell through an oxygen concentration sensor. When the oxygen concentration exceeds a set threshold (usually 1%), it automatically replenishes the shell with inert gas while simultaneously expelling excess gas, thereby maintaining a stable anaerobic environment within the shell.

[0019] All of the above subsystems—sample processing, pneumatic stirring, multi-stage filtration, bacterial culture collection, temperature control, and gas control—are electrically connected to a central control system. This control system automatically coordinates the start-up, operation, and shutdown of each subsystem according to a preset program sequence, achieving fully automated operation from sample addition to collection.

[0020] Preferably, the angle between the gas nozzle's injection direction and the tangential direction of the sample container's inner wall is set to 30° to 60°. When the gas exits from the nozzle, its velocity vector can be decomposed into a component tangential to the wall and a component pointing towards the center of the container. These two components work together to form a controllable swirling field. If the angle is too large, the tangential component is insufficient, weakening the swirling effect; if the angle is too small, the center-pointing component is insufficient, resulting in poor liquid turbulence. Fluid dynamics simulations have determined that 45° is the optimal angle.

[0021] Preferably, the pneumatic stirring system also includes a pulse generator installed on the gas delivery pipeline. The pulse frequency generated by this device is adjustable within the range of 0.5-5Hz. Compared to continuous airflow, pulsed airflow can generate richer vortex structures in the liquid, which is beneficial for eliminating mixing dead zones.

[0022] Preferably, the multi-stage gradient filtration system consists of a first-stage, second-stage, and third-stage filtration units. The first-stage filter media has a pore size of 200-500 μm and is made of 304 stainless steel woven mesh, used to trap large particles such as undigested plant and meat fibers. The second-stage filter media has a pore size of 50-100 μm and is also made of stainless steel mesh, used to remove medium-sized particles. The third-stage filter media has a pore size of 10-30 μm and is made of polyethersulfone (PES) microporous membrane, used for final fine filtration, trapping fine impurities while allowing bacteria to pass through.

[0023] Preferably, the filter units at each stage employ a detachable, sealed connection structure, such as quick-release clamps or threaded joints. This design allows each filter unit to be independently disassembled, replaced, or cleaned, facilitating adjustments to the filtration configuration based on the actual conditions of the sample and reducing maintenance costs.

[0024] Preferably, the filtration system is also equipped with a backwashing unit. This unit is connected to the liquid outlet side of each stage of the filtration unit via piping. After filtration is complete, it applies a liquid or airflow in the opposite direction to the normal filtration to the filter media, flushing away any residue adhering to the surface of the filter media. This function is of practical significance for extending the service life of the filter media and maintaining the stability of the filtration flux.

[0025] Preferably, the temperature control system has a target temperature range of 2-8℃. This temperature range is determined based on the low-temperature tolerance and metabolic inhibition characteristics of the dominant genera in the intestinal flora (such as Bacteroides and Firmicutes). At this temperature, the metabolic activity of the flora is significantly reduced, but the cell membrane fluidity is not irreversibly damaged, effectively maintaining the activity of the flora during the separation process.

[0026] Preferably, the gas control system maintains the oxygen concentration inside the casing below 1%. This threshold is based on the sensitivity of strict anaerobes (such as Bifidobacteria, Lactobacillus, etc.) to oxygen. When the oxygen concentration is below 1%, the survival rate of most strict anaerobes can be maintained at a high level.

[0027] Preferably, a liquid level sensor is installed inside the sample container. This sensor is electrically connected to the control system to monitor the liquid level in the container in real time, preventing excessive or insufficient diluent injection, and also providing a liquid volume reference for subsequent filtration steps.

[0028] Preferably, the housing is equipped with a visual observation window made of transparent material (such as tempered glass or polycarbonate), which allows operators to observe the internal operating status without opening the sealed cover, so as to detect abnormalities in a timely manner.

[0029] Preferably, this device is also equipped with a deodorization unit, which is connected to the internal space of the casing. This unit uses an activated carbon adsorption layer as the main deodorization medium to adsorb and decompose odorous gases that may escape from the casing, thereby improving the operating environment.

[0030] Preferably, the sealing cover is equipped with an electromagnetic locking mechanism, which is electrically connected to the control system. During device operation, the locking mechanism automatically locks to prevent operators from accidentally opening the sealing cover, which could disrupt the anaerobic environment or cause personal injury. After operation, the control system sends an unlocking signal, allowing the locking mechanism to open.

[0031] (III) Beneficial Effects Compared with the prior art, the present invention provides a casting apparatus for manufacturing metal products, which has the following advantages: 1. In terms of mixing method, pneumatic swirling mixing replaces traditional mechanical mixing. This change fundamentally eliminates the impact of motor heat on the temperature of the bacterial solution and avoids shear damage to the bacterial community caused by the mixing blades. Furthermore, compared to existing bubble mixing methods, the tangential swirling mixing with pulse perturbation method of this invention has higher mixing efficiency and better repeatability.

[0032] 2. In terms of filtration strategy, a three-stage gradient filtration system combined with negative pressure drive is adopted to achieve a balance between high throughput and high precision. The filter media at each stage are arranged according to the pore size gradient, large particles are intercepted in the primary stage, and fine particles are removed in the final stage. Each stage can play its most suitable separation role, resulting in a significant improvement in overall filtration efficiency.

[0033] 3. In terms of environmental control, the low-temperature constant temperature and anaerobic maintenance functions are integrated into the same shell, providing a stable physicochemical environment for the bacterial community throughout the entire bacterial solution preparation process. This integrated design not only simplifies the operation process but also fundamentally ensures the consistency of bacterial activity and therapeutic effect in the final product.

[0034] 4. In terms of operational procedures, a fully enclosed automated operation has been achieved from sample addition to collection. Operators only need to place the sample into the container at the beginning and remove the collection bottle afterward; no manual intervention is required in between. This not only reduces operational difficulty and labor intensity but also minimizes the opportunity for samples to come into contact with operators, thus improving working conditions. Attached Figure Description

[0035] Figure 1 is a schematic diagram of the overall appearance structure of this device; Figure 2 is a schematic diagram of the longitudinal cross-sectional structure of this device; Figure 3 is a partially enlarged schematic diagram of the sample processing system of this device; Figure 4 is a side view of the gas nozzle sidewall arrangement of this device; Figure 5 is a top-view schematic diagram of the swirling flow field in the sample container of this device; Figure 6 is a schematic diagram of the overall assembly of the multi-stage gradient filtration system of this device; Figure 7 is a schematic diagram of the explosive decomposition of a single-stage filtration unit of this device; Figure 8 is a schematic diagram of the bacterial culture collection system of this device; Figure 9 is a block diagram of the signal principle of the temperature control system and gas control system of this device; Figure 10 is a block diagram of the electrical connection of the overall control system of this device.

[0036] In the diagram: 1. Housing; 101. Accommodation space; 102. Observation window; 103. Sealing element; 2. Sealing cap; 201. Electromagnetic locking mechanism; 3. Sample processing system; 301. Sample container; 301a. Sample inlet; 301b. Sample outlet; 302. Diluent injection unit; 302a. Diluent storage tank; 302b. Injection pipeline; 302c. Injection pump (peristaltic pump); 303. Homogenization unit; 303a. Homogenizing impeller; 303b. Homogenizing drive motor; 304. Liquid level sensor (float type or capacitive type); 4. Pneumatic stirring system; 401. Gas generator (nitrogen cylinder or small air compressor); 402. Gas delivery pipeline; 403. Gas nozzle (stainless steel, inner diameter 2.5mm); 404. Pulse generator (electromagnetic pulse valve); 405. Gas filter (0.22μm sterile grade); 406. Gas flow regulating valve (precision needle valve); 5. Multi-stage gradient filtration system; 501, First-stage filtration unit; 501a, First filter medium (300-mesh stainless steel mesh); 501b, First housing (transparent polycarbonate); 501c, First inlet; 501d, First outlet; 502, Second-stage filtration unit; 502a, Second filter medium (150-mesh stainless steel mesh); 502b, Second housing; 502c, Second inlet; 502d, Second outlet; 503, Third... First-stage filtration unit; 503a, third filter medium (PES microporous membrane, pore size 20μm); 503b, third housing; 503c, third inlet; 503d, third outlet; 504, connecting pipeline (silicone hose); 505, sealing connection structure (quick-connect clamp); 506, backwashing unit; 506a, backwashing liquid source (sterile saline bag); 506b, backwashing pipeline; 506c, backwashing control valve (solenoid clamp valve); 6. Bacterial liquid collection system; 601. Collection container (sterile glass bottle); 601a. ​​Bacterial liquid collection port; 601b. Negative pressure interface; 602. Negative pressure generating device (diaphragm vacuum pump); 603. Bacterial liquid output pipeline; 603a. Output control valve (solenoid clamp valve); 7. Temperature control system; 701. Temperature sensor (PT100 platinum resistance thermometer); 702. Refrigeration unit (semiconductor cooling chip, TEC1, 12706); 703. Heating unit (PTC heating element); 704. Temperature controller (PID control module); 8. Gas control system; 801. Oxygen concentration sensor (electrochemical type, range 0, 25%); 802. Inert gas supply unit; 802a. Inert gas storage tank (nitrogen cylinder, 5L); 802b. Gas supply pipeline; 802c. Gas supply control valve (solenoid valve); 803. Gas circulation unit (miniature axial flow fan); 804. Gas exhaust unit (solenoid exhaust valve); 9. Control system; 901. Central processing unit (STM32 series microcontroller); 902. Storage unit (SD card module); 903. Display unit (7-inch TFT touch screen); 904. Operation panel (membrane buttons); 10. Deodorization unit; 1001. Activated carbon adsorption layer (granular activated carbon, iodine value ≥1000); 1002. Exhaust pipe. Detailed Implementation

[0037] The technical solution of the present invention will be described in detail below with reference to the accompanying drawings and specific embodiments. It should be noted in advance that the following embodiments are only used to help understand the technical essence of the present invention and should not be construed as limiting the scope of protection of the present invention. Various equivalent transformations or modifications made by those skilled in the art based on the concept of the present invention after reading the content of this invention all fall within the scope of protection defined by the claims of this invention.

[0038] Description of the overall structure of the device like Figure 1 and Figure 2 As shown, the fecal microbial transplantation and bacterial culture separation device provided in this embodiment has an overall vertical cabinet design, with external dimensions of approximately 450mm wide, 500mm deep, and 800mm high, which can be easily placed on a laboratory bench or a mobile cart. The shell 1 is made of SUS304 stainless steel plate by bending and welding, with a wall thickness of 1.5mm. Both the inner and outer surfaces are brushed and passivated, which has good corrosion resistance and is easy to clean.

[0039] An observation window 102 is embedded in the center of the front wall of the housing 1. This window is made of 10mm thick transparent tempered glass and measures 200mm × 300mm. Its installation position corresponds to that of the sample container 301, allowing operators to easily observe the liquid level and agitation within the sample container. A transparent anti-fog film is applied to the inside of the observation window 102 to prevent fogging on the glass surface due to temperature differences, which would affect observation.

[0040] A flange is machined at the top opening of the housing 1, and an annular sealing groove is formed on the flange surface. The sealing element 103 (using a silicone rubber O-ring with a cross-sectional diameter of 5mm) is embedded in the sealing groove. The sealing cover 2 is a stainless steel stamped part, which is connected to the top flange of the housing 1 by a hinge. An electromagnetic locking mechanism 201 is provided at the leading edge of the sealing cover 2. When energized, this mechanism generates a suction force to firmly press the sealing cover 2 onto the sealing element 103, ensuring the airtightness of the housing. The suction force of the electromagnetic locking mechanism 201 is designed to be no less than 50N, which is sufficient to resist the slight positive pressure (usually not exceeding 5kPa) generated during gas replacement inside the housing. When the equipment is in operation, the electromagnetic locking mechanism 201 is locked by the control system 9, and the operator cannot open the sealing cover 2; after operation, the control system 9 automatically cuts off the power to the electromagnetic locking mechanism 201, at which point the operator can manually open it.

[0041] The internal accommodating space 101 of the housing 1 is functionally divided into three areas: upper, middle, and lower. The upper area is mainly used to house the upper piping of the sample processing system 3 and the pneumatic stirring system 4; the middle area is used to house the multi-stage gradient filtration system 5; and the lower area is used to house the bacterial culture collection system 6 and some auxiliary equipment. The cooling unit 702 and heating unit 703 of the temperature control system 7 are attached to the inner side of the rear wall of the housing 1. The oxygen concentration sensor 801 of the gas control system 8 is installed on the middle side wall of the accommodating space 101. The gas supply port of the inert gas supply unit 802 is located in the top area of ​​the accommodating space 101, which facilitates the inert gas to push air out from top to bottom.

[0042] Specific implementation methods of the sample processing system like Figure 2 and Figure 3 As shown, the sample processing system 3 includes a sample container 301, a diluent injection unit 302, and a homogenization unit 303.

[0043] Sample container 301 is integrally molded from transparent polycarbonate (PC) material using an injection molding process. It has a wall thickness of 3mm and a capacity of 1500mL. The container is cylindrical with an inner diameter of 120mm and a height of 150mm. The bottom of the container is designed to be slightly conical (with a cone angle of approximately 15°) to facilitate complete drainage of the liquid. The sample inlet 301a is located at the center of the top of the container, with an inner diameter of 50mm, and is equipped with an independent screw cap. The operator opens the cap when adding the sample and tightens it afterward to seal it. The discharge outlet 301b is located at the apex of the cone at the bottom of the container, with an inner diameter of 10mm. It is connected to the first-stage inlet of the multi-stage gradient filtration system 5 via a silicone hose. An electromagnetic clamp valve (not shown in the figure) is installed on the hose to control the release of the liquid.

[0044] The diluent injection unit 302 consists of a diluent storage tank 302a, an injection line 302b, and an injection pump 302c. The diluent storage tank 302a is made of medical-grade polypropylene (PP) with a capacity of 2000 mL. It is pre-filled with sterile physiological saline (0.9% NaCl solution) or phosphate buffered saline (PBS, pH 7.2) and is placed on the outside of the housing 1, utilizing gravity to assist in liquid supply, while the flow rate is precisely controlled by the injection pump 302c. The injection pump 302c is a peristaltic pump with a flow rate range of 0-500 mL / min and a control accuracy of ±5 mL. The injection line 302b is a silicone tube with an inner diameter of 6 mm. One end is immersed below the liquid surface in the storage tank, and the other end passes through the sealed interface of the housing 1 into the sample container 301. The tube opening is fixed to the upper part of the container's inner wall.

[0045] The homogenization unit 303 consists of a homogenizing impeller 303a and a homogenizing drive motor 303b. The homogenizing impeller 303a is made of sanitary stainless steel (SUS316L) and comprises a central shaft and three layers of impeller blades. The central shaft has a diameter of 6mm and a length of 180mm. The three layers of blades are located at the bottom, middle, and lower-middle parts of the shaft, respectively. Each layer contains four blades, each 15mm wide and 1mm thick, with a spacing of 35mm between layers. Adjacent layers are staggered by 45° in the circumferential direction. This multi-layered blade arrangement facilitates effective shearing and mixing at different liquid levels. The homogenizing drive motor 303b is a brushless DC motor with a rated power of 60W and a maximum speed of 3000rpm. Power is transmitted to the impeller shaft via a magnetic coupler (not shown in the figure) to avoid the sealing problems caused by the motor shaft directly penetrating the container. The magnetic coupler design ensures that the sample container 301 maintains a completely sealed structure.

[0046] The liquid level sensor 304 employs a capacitive liquid level switch. Its sensing probe is inserted obliquely into the sample container 301 from the side wall, with the probe tip flush with the inner wall of the container. The sensor has a detection accuracy of ±2mm and a response time of less than 0.5s. Its signal output terminal is connected to the analog input port of the control system 9.

[0047] Specific implementation methods of pneumatic stirring systems like Figure 4 and Figure 5 As shown, the pneumatic stirring system 4 is one of the key modules that distinguishes this device from existing technologies.

[0048] In this embodiment, the gas generating device 401 is a small oil-free air compressor with a rated discharge pressure of 0.6 MPa and a discharge volume of 15 L / min. A refrigerated dryer and a three-stage filtration system (with filtration accuracies of 5 μm, 0.3 μm, and 0.01 μm respectively) are installed at the compressor outlet to ensure that the output compressed air is dry and clean. For applications with strict oxygen requirements, nitrogen cylinders can be used as the gas source. The gas delivery pipeline 402 uses polytetrafluoroethylene (PTFE) tubing with an outer diameter of 8 mm and an inner diameter of 5 mm. This material has good pressure resistance and chemical stability and does not adsorb bacteria or proteins.

[0049] Four gas nozzles 403 are provided, evenly distributed circumferentially on the lower side wall of the sample container 301, with an included angle of 90° between adjacent nozzles. The centerline of each nozzle is 30mm from the bottom of the container, and the inner diameter of the nozzle outlet is 2.5mm. Figure 5 As shown, the angle α between the axial direction of each nozzle 403 and the tangential direction of the container wall at that point is set to 45°. When the compressed gas is ejected from the nozzle at a certain speed, its velocity vector is decomposed into a tangential component and a component pointing towards the center of the container. The former drives the liquid to move in a circular motion along the wall, while the latter causes the liquid to flow centripetally. The combination of the two forms a flow field structure similar to a Rankine vortex, where the peripheral fluid undergoes forced vortex motion, and the central region undergoes a solid-like rotation. This flow field structure is beneficial for the full exchange of substances between the liquids at different locations within the container.

[0050] On the gas delivery pipeline 402, a gas filter 405 (0.22μm pore size, polyvinylidene fluoride membrane), a gas flow regulating valve 406 (precision needle valve, adjustment range 0-10L / min), and a pulse generator 404 are sequentially connected in series from the gas generator 401 to the nozzle 403. The pulse generator 404 uses an electromagnetic pulse valve, which works by periodically and rapidly opening and closing the flow channel under the control of the electromagnetic valve, cutting the continuous airflow into a series of discrete gas pulses. In this embodiment, the pulse frequency is set to 2Hz, that is, two injections and two pauses per second, with each injection lasting approximately 150ms. It should be noted that the pulse frequency is not a fixed value and can be adjusted by the operator within the range of 0.5-5Hz according to the viscosity of the sample. For samples with high viscosity (such as feces from constipated patients), appropriately increasing the pulse frequency can enhance the intensity of the disturbance; for samples with low viscosity (such as feces from diarrheal patients), the frequency can be reduced to decrease excessive impact on the liquid surface.

[0051] Typical operating parameters for pneumatic agitation are as follows: injection pressure 0.2-0.3 MPa, single agitation duration 5-8 min. In actual use, the agitation effect can be directly evaluated through observation window 102—when the liquid surface exhibits a stable vortex shape and the liquid surface height fluctuation is less than ±5 mm, it indicates that the mixing has reached a uniform state.

[0052] Specific implementation methods of multi-level gradient filtering systems like Figure 6 and Figure 7 As shown, the multi-stage gradient filtration system 5 consists of three independent filtration units connected in series, namely the first-stage filtration unit 501, the second-stage filtration unit 502, and the third-stage filtration unit 503.

[0053] The first-stage filter unit 501 primarily performs coarse filtration. Its first housing 501b is injection-molded from transparent polycarbonate material, forming a cylindrical shape with an inner diameter of 60mm and a height of 80mm. The first filter medium 501a is a stainless steel woven mesh with a pore size of 300μm (equivalent to 50 mesh), providing an effective filtration area of ​​approximately 28cm². 2 The choice of pore size is based on statistical analysis of the particle size distribution of solid particles in fecal samples. According to our screening experiments on 120 fecal samples from healthy donors, particles larger than 300 μm mainly consist of undigested plant cell walls, fiber bundles, and a small amount of muscle fibers, which account for approximately 15%-25% of the total solid weight of the feces. Removing this portion in the primary filtration creates favorable preconditions for subsequent fine filtration.

[0054] The structure of the second-stage filter unit 502 is basically similar to that of the first stage. Its second housing 502b also has an inner diameter of 60mm and a height of 70mm. The second filter medium 502a is a stainless steel woven mesh with a pore size of 80μm (equivalent to 180 mesh), and an effective filtration area of ​​approximately 26cm². 2 This stage is used to retain medium-sized particles with a diameter between 80 and 300 μm, mainly including some starch particles, small-sized plant fibers, and detached intestinal epithelial cell fragments.

[0055] The third-stage filtration unit 503 employs microporous membrane filtration. Its third housing 503b is a detachable flat-sheet membrane assembly structure, internally containing a support plate and a flow guiding layer. The third filter medium 503a is a polyethersulfone (PES) microporous membrane with a pore size of 20 μm and a diameter of 50 mm, providing an effective filtration area of ​​approximately 19.6 cm². 2 This material exhibits excellent hydrophilicity and low protein adsorption properties, making it suitable for filtering liquids containing bioactive components. Its 20μm pore size allows the vast majority of intestinal flora (typically between 0.5-10μm in size) to pass through smoothly, while retaining residual impurities larger than 20μm (such as incompletely dispersed mucus clumps, mineral particles, etc.).

[0056] It should be noted that the pore size values ​​given above (300μm→80μm→20μm) are only specific implementations of this embodiment. In practical applications, the pore size at each stage can be adjusted according to the characteristics of the fecal sample. For example, for hard stools with high formability, the primary pore size can be appropriately increased (up to 500μm) to avoid excessive clogging; for loose or watery stools, the pore size at each stage can be appropriately reduced to achieve higher final liquid purity. The connection between each filtration unit adopts a quick-release clamp-type sealing connection structure 505. Each unit's inlet and outlet are equipped with standard clamp connectors. During assembly, the units are tightened by silicone sealing gaskets, and during disassembly, any unit can be removed simply by loosening the clamps.

[0057] The filtration process is powered by a negative pressure generator 602 in the bacterial culture collection system 6. For example... Figure 8 As shown, the negative pressure generating device 602 uses a diaphragm vacuum pump with a maximum vacuum of -85 kPa and a pumping rate of 15 L / min. In this embodiment, the working negative pressure is set to -50 kPa. Actual testing showed that under this negative pressure, 1500 mL of fecal suspension (with a solids content of approximately 10%) completes three-stage filtration in approximately 8-12 minutes, with a filtration flux of approximately 125-188 mL / min. It should be noted that as filtration progresses, a filter cake layer gradually accumulates on the surface of the filter media, increasing filtration resistance and reducing flux. When the flux drops below 60% of its initial value, the control system 9 will prompt the operator to initiate the backwashing procedure.

[0058] The backwash unit 506 is designed to address the aforementioned flux decay issue. After each filtration cycle, the control system 9 automatically initiates the backwashing procedure: the backwash control valve 506c opens, and the liquid in the backwash source 506a (in this embodiment, a 500mL bag of sterile saline solution suspended above the outer shell) enters the outlet side of each stage of the filtration unit under gravity via the backwash pipeline 506b, passing through the filter media in the opposite direction to the normal filtration. The backwash flow rate is approximately 200mL / min, lasting for 30 seconds. This reverse flow partially disperses the filter cake layer adhering to the surface of the filter media, causing trapped particles to detach from the media surface, thereby restoring the flux of the filter media. The waste liquid generated during backwashing is discharged from the inlet side and enters the waste collection container.

[0059] Specific implementation methods for the bacterial culture collection system like Figure 8 As shown, the collection container 601 of the bacterial culture collection system 6 is made of heat-resistant glass, with a volume of 500 mL. The bottle mouth is a standard GL45 threaded cap, equipped with a sealing cap with two interfaces. One interface is the bacterial culture collection port 601a, which connects to the outlet of the third-stage filtration unit 503 via a silicone tubing; the other interface is the negative pressure port 601b, which connects to the suction port of the negative pressure generating device 602 via a silicone tubing. The collection container 601 undergoes dry heat sterilization (180℃, 2h) before use to ensure sterility.

[0060] An output control valve 603a (solenoid clamp valve) is installed on the bacterial culture output line 603 to close the line after bacterial culture collection is completed, preventing backflow. After collection, the operator can close the output control valve 603a, disconnect the collection container 601 from the line, remove the collection container 601, and transfer it to a sterile operating table for further processing (such as dispensing or freezing). For easy handling, the collection container 601 is placed on a removable sliding tray.

[0061] Specific implementation methods for temperature control systems and gas control systems like Figure 9 As shown, the core control logic of the temperature control system 7 is PID (proportional-integral-derivative) feedback control. The temperature sensor 701 (PT100 platinum resistance thermometer, accuracy class A) is installed in the central area of ​​the accommodating space 101. The temperature signal detected by the sensor is converted by a transmitter and input to the temperature controller 704. The temperature controller 704 compares the actual temperature with the set value (4℃ in this embodiment), calculates the deviation, and outputs a control signal to the refrigeration unit 702 or the heating unit 703.

[0062] The cooling unit 702 uses two semiconductor cooling chips (TEC1-12706 type, maximum cooling power 60W), which are attached to the inner rear wall and the inner left side wall of the housing 1 respectively. The hot end of the cooling chip is in close contact with the aluminum alloy heat sink, and an axial fan is installed on the outside of the heat sink to dissipate heat to the outside of the housing. The heating unit 703 uses a PTC (positive temperature coefficient) ceramic heating element with a rated power of 50W, which is installed at the bottom of the housing 1. When the temperature inside the housing is higher than the set value, the PID controller outputs a cooling signal; when the temperature is lower than the set value, it outputs a heating signal. After actual testing, this temperature control system can stabilize the temperature inside the accommodating space 101 within the range of 4±0.5℃, and the temperature fluctuation is significantly smaller than that of a simple temperature control scheme using on / off control (typically with a fluctuation range of ±2℃).

[0063] The working process of the gas control system 8 is as follows: In the initial stage after the device is started, the gas supply control valve 802c of the inert gas supply unit 802 opens, and nitrogen (purity ≥99.9%) enters the top area of ​​the containment space 101 from the inert gas storage tank 802a (5L steel cylinder, initial pressure 15MPa, reduced to 0.1MPa by a secondary pressure reducing valve) via the gas supply pipeline 802b. Since the density of nitrogen is slightly less than that of air, it gradually pushes the air downwards after entering from the top, and finally the displaced air is discharged outside the housing through the exhaust valve 804a of the gas exhaust unit 804. The oxygen concentration sensor 801 (electrochemical type, measurement range 0-25%, accuracy ±0.5%FS) monitors the oxygen content in the containment space 101 in real time. When the oxygen content drops below 1%, the gas supply control valve 802c closes, and the exhaust valve 804a closes simultaneously, and the housing enters a sealed state.

[0064] During operation, oxygen from the outside air will not seep in due to the sealing effect of the sealing cover 2. However, considering the possibility of minor leaks at the pipe openings and interfaces on the casing, the oxygen concentration sensor 801 continues to operate. When the detected oxygen content rises above 1.5%, the control system 9 automatically opens the gas supply control valve 802c and the exhaust valve 804a for a short period of ventilation until the oxygen content drops back below 1%. Gas circulation unit 803 (miniature axial flow fan, air volume 0.5m³ / h) 3 The system operates continuously during operation ( / min) to promote gas flow within the containment space 101 and prevent local oxygen enrichment caused by density stratification.

[0065] Specific implementation methods of the control system like Figure 10As shown, the control system 9 is based on a central processing unit 901 (using an STM32F407 series ARM Cortex-M4 core microcontroller with a main frequency of 168MHz), an external storage unit 902 (containing 256KB of EEPROM for storing key parameters and a 4GB SD card for recording operation logs), a display unit 903 (7-inch TFT color touch screen with a resolution of 800×480), and an operation panel 904 (including three physical buttons for start, stop, and emergency stop, as well as a digital encoder knob for parameter setting).

[0066] The digital output ports of the central processing unit 901 are connected to: the start / stop control terminal of the homogenizing drive motor 303b, the enable terminal of the injection pump 302c, the drive coil of the electromagnetic locking mechanism 201, and the control terminals of each solenoid valve (including the gas supply control valve 802c, the exhaust valve 804a, the backwash control valve 506c, the output control valve 603a, etc.). The analog input ports of the central processing unit 901 are connected to: the signal conditioning output terminal of the temperature sensor 701, the signal output terminal of the oxygen concentration sensor 801, and the signal output terminal of the liquid level sensor 304. The PWM (pulse width modulation) output port of the central processing unit 901 is connected to: the control terminal of the pulse generator 404 (used to adjust the pulse frequency and duty cycle), and the drive circuits of the cooling unit 702 and the heating unit 703 (used for power regulation).

[0067] At the software level, the control system 9 has a pre-set standard operating procedure. After the operator selects the "standard mode" through the touch screen, he only needs to press the "start" button on the operation panel 904, and the device will run automatically in the following sequence: (1) the electromagnetic locking mechanism 201 is locked; (2) the gas control system 8 is started to perform anaerobic replacement; (3) the temperature control system 7 is started to start cooling; (4) the diluent injection unit 302 is started to inject a predetermined volume of diluent (usually 3-5 times the sample volume); (5) the homogenization unit 303 is started to stir for 2-5 minutes; (6) the pneumatic stirring system 4 is started to stir with pulsed airflow for 5-10 minutes; (7) the negative pressure generating device 602 is started to start filtration; (8) after the bacterial solution is collected, the output control valve 603a is closed; (9) the backwashing unit 506 is started to complete backwashing; (10) all systems stop, the electromagnetic locking mechanism 201 is unlocked, and the touch screen displays "running completed".

[0068] Throughout the process, the display unit 903 updates the following parameters in real time: current temperature, oxygen concentration, liquid level, cumulative filtration time, and negative pressure value. This data is synchronously stored on an SD card, forming a complete operation log for easy traceability and quality control.

[0069] Specific implementation methods of the deodorization unit The deodorization unit 10 is connected to the top area of ​​the accommodating space 101 via an exhaust pipe 1002. When the internal gas pressure is slightly higher than atmospheric pressure due to inert gas displacement (or when the exhaust valve 804a is actively opened), the gas first passes through the activated carbon adsorption layer 1001. This adsorption layer is filled with granular coconut shell activated carbon, with an iodine adsorption value ≥1000mg / g, a filling thickness of 50mm, and an effective filtration area of ​​approximately 150cm². 2 The odor components in the gas (mainly sulfur-containing compounds such as hydrogen sulfide and methanethiol, and nitrogen-containing compounds such as indole and skatole) are removed by physical and chemical adsorption as they pass through the activated carbon layer. The deodorized gas is then discharged into the laboratory fume hood or outdoor environment through exhaust pipe 1002, which can significantly improve the working experience of operators.

[0070] Example 1: Isolation of bacterial culture from fecal samples from healthy donors In this embodiment, the above-described device was used to perform bacterial separation on a fecal sample from a healthy donor to verify the basic function of the device.

[0071] The donor was a 32-year-old healthy male with no history of antibiotic use and normal bowel movement frequency (once daily). Approximately 50g of fresh feces was collected and immediately placed in an ice pack for transport to the laboratory. The sealing cap 2 of the device was opened, and the fecal sample was inserted into sample container 301 through the inlet 301a. The inlet cap was tightened, and the sealing cap 2 was closed. The following parameters were set on the touchscreen: diluent volume 250mL (liquid-to-solid ratio 5:1), homogenization speed 1500rpm, homogenization time 3min, pneumatic stirring pressure 0.25MPa, pulse frequency 2Hz, stirring time 6min, negative pressure -50kPa. The "Start" button was then pressed.

[0072] The device operates automatically according to a preset program. Through observation window 102, it can be seen that during the pneumatic stirring stage, a distinct vortex flow field is formed within the sample container 301, with the liquid surface sinking by approximately 20 mm at the center and rising by approximately 5 mm at the edges, indicating that the tangential vortex flow has been successfully established. During the filtration stage, the liquid level decreases at a rate of approximately 2 mm / min, and the filtration process is stable.

[0073] The entire process took approximately 22.5 minutes: anaerobic replacement (3 min) + homogenization (3 min) + pneumatic stirring (6 min) + filtration (10 min) + backwashing (0.5 min). The total collected bacterial culture was approximately 190 mL. Samples were taken for viable cell counting using anaerobic culture (Brucella blood agar plates, 37℃, anaerobic incubation for 72 h). The results showed a viable cell count of 2.3 × 10⁻⁶. 9 CFU / mL. Using the same stool sample as a control, a traditional manual method (magnetic stirring + two layers of gauze filtration) was employed, yielding a viable bacterial count of 6.7 × 10⁻⁶. 8CFU / mL. The results obtained by this device were approximately 3.4 times that of the traditional method, indicating that the combination of pneumatic stirring and a low-temperature anaerobic environment has a significant protective effect on bacterial activity.

[0074] Example 2: Adaptability verification of fecal samples with different characteristics Considering the significant differences in characteristics between fecal samples from different donors or from the same donor at different times, this embodiment examines the adaptability of the device to samples with different characteristics.

[0075] Three representative fecal samples were selected: Sample A was normal formed stool (Bristol type 4), Sample B was pasty stool (Bristol type 6), and Sample C was hard, spherical stool (Bristol type 2). 50g of each sample was taken and processed using the device. The pneumatic stirring parameters were adjusted for different samples: Standard parameters (pressure 0.25MPa, frequency 2Hz) were used for Sample A; for Sample B, due to its lower viscosity, the pressure was reduced to 0.18MPa and the frequency increased to 3Hz to avoid excessive foaming; for Sample C, due to its higher viscosity, the pressure was increased to 0.35MPa and the frequency decreased to 1Hz to ensure sufficient shear force.

[0076] All three experiments successfully separated the bacterial culture. The filtration times were: Sample A 10 min, Sample B 7 min, and Sample C 16 min. The viable cell counts in the collected bacterial cultures were 2.3 × 10⁻⁶. 9 CFU / mL, 3.1×10 9 CFU / mL and 1.2×10 9 CFU / mL. The results showed that for loose stool samples, due to lower solid content and fewer particulate matter, the filtration speed was fastest, resulting in the highest viable bacteria concentration. For hard stool samples, longer stirring time was required for thorough dispersion, and the higher fiber content led to a slower filtration speed, resulting in a lower final viable bacteria concentration, but it was still significantly higher than the results obtained by traditional methods for the same sample (traditional methods typically yield viable bacteria counts below 5 × 10⁻⁶ for hard stool samples). 8 (CFU / mL). This embodiment demonstrates that by flexibly adjusting the pneumatic stirring parameters, this device can adapt to fecal samples with different characteristics, exhibiting strong versatility.

[0077] Example 3: Verification of the backwashing unit's effect on maintaining filtration flux This embodiment aims to verify the actual effect of the backwashing unit 506 in maintaining filtration throughput and extending the service life of the filter media.

[0078] Using the same set of filter media (three-stage filter / membrane), six fecal samples (50g feces per sample, with 250mL of diluent) were processed consecutively. After each sample was processed, the filtration time of each stage of the filter unit was recorded (measured by the time required for the liquid to completely pass through the three-stage filter). The first three samples were not backwashed after processing (control group), while the last three samples were backwashed according to standard (experimental group, 200mL backwash solution, 30s).

[0079] Control group results: The filtration time for the first sample was 9.5 min, the second sample was increased to 14 min, and the third sample was increased to 21 min but still could not be completed, and the experiment was stopped due to excessively low flux. Experimental group results: The filtration time for the first sample was 10 min, the second sample was 11 min, and the third sample was 10.5 min. There was no statistically significant difference between the three runs (P>0.05), indicating that the backwashing operation effectively restored the flux of the filter media. Further disassembly and observation of the filter media surface revealed that the surface of the unbackwashed third-stage microporous filter membrane was covered with a dense yellowish-brown filter cake layer, and the light transmittance was significantly reduced; while the surface of the filter membrane after backwashing only had a small amount of loose particles remaining, and the light transmittance was basically restored to the initial state.

[0080] The results of this embodiment demonstrate that the backwashing unit plays a crucial role in maintaining batch-to-batch consistency of the device. In practical clinical applications, if multiple batches of bacterial culture need to be prepared for each donor sample, the backwashing function can significantly improve work efficiency and avoid increased costs and operational steps due to frequent filter media replacements.

Claims

1. A bacterial culture separation device for fecal microbiota transplantation, characterized in that, include: The shell (1) is a closed box structure with an internal accommodating space (101). A sealing cap (2) is disposed on the top of the housing (1) and is airtightly connected to the housing (1) by a sealing element (103); A sample processing system (3) is disposed within the accommodating space (101), comprising a sample container (301), a diluent injection unit (302), and a homogenizing unit (303); the sample container (301) has an inlet (301a) at the top and an outlet (301b) at the bottom; the diluent injection unit (302) is connected to the sample container (301); and the homogenizing unit (303) is disposed within the sample container (301). The pneumatic stirring system (4) includes a gas generating device (401), a gas delivery pipeline (402), and a gas nozzle (403) disposed on the inner wall of the sample container (301). The gas generating device (401) is connected to the gas nozzle (403) through the gas delivery pipeline (402). A multi-stage gradient filtration system (5) is located downstream of the sample container (301). It includes at least three sequentially connected filtration units. Each filtration unit is provided with a filter medium with a different pore size. Along the liquid flow direction, the pore size of the filter medium decreases step by step. The bacterial liquid collection system (6) is located downstream of the multi-stage gradient filtration system (5), and includes a collection container (601) and a negative pressure generating device (602) connected to the collection container (601). The collection container (601) is connected to the outlet of the last stage filtration unit. The temperature control system (7) includes a temperature sensor (701), a refrigeration unit (702), a heating unit (703), and a temperature controller (704) for regulating the temperature within the accommodating space (101); The gas control system (8) includes an oxygen concentration sensor (801), an inert gas supply unit (802), and a gas circulation unit (803) for regulating the gas composition within the accommodating space (101); The control system (9) is electrically connected to the sample processing system (3), the pneumatic stirring system (4), the multi-stage gradient filtration system (5), the bacterial liquid collection system (6), the temperature control system (7), and the gas control system (8), respectively.

2. The fecal microbiota transplantation bacterial solution separation device according to claim 1, characterized in that, The angle between the injection direction of the gas nozzle (403) and the tangential direction of the inner wall of the sample container (301) is 30° to 60°.

3. The fecal microbiota transplantation bacterial solution separation device according to claim 1, characterized in that, The pneumatic stirring system (4) further includes a pulse generator (404) installed on the gas delivery pipeline (402), and the pulse frequency output by the pulse generator (404) is 0.5-5Hz.

4. The fecal microbiota transplantation bacterial solution separation device according to claim 1, characterized in that, The multi-stage gradient filtration system (5) includes a first-stage filtration unit (501), a second-stage filtration unit (502), and a third-stage filtration unit (503); the first filter medium (501a) in the first-stage filtration unit (501) has a pore size of 200-500μm; the second filter medium (502a) in the second-stage filtration unit (502) has a pore size of 50-100μm; and the third filter medium (503a) in the third-stage filtration unit (503) has a pore size of 10-30μm.

5. The fecal microbiota transplantation bacterial solution separation device according to claim 1, characterized in that, The filter units at each level of the multi-stage gradient filtration system (5) are connected by a detachable sealed connection structure (505).

6. The fecal microbiota transplantation bacterial solution separation device according to claim 1, characterized in that, The multi-stage gradient filtration system (5) further includes a backwashing unit (506), which is connected to each stage of the filtration unit and is used to apply reverse liquid flow or reverse airflow to the filter medium.

7. The fecal microbiota transplantation bacterial solution separation device according to claim 1, characterized in that, The temperature control system (7) maintains the temperature within the accommodating space (101) at 2-8°C.

8. The fecal microbiota transplantation bacterial solution separation device according to claim 1, characterized in that, The gas control system (8) maintains the oxygen concentration in the containment space (101) below 1%.

9. The fecal microbiota transplantation bacterial solution separation device according to claim 1, characterized in that, A liquid level sensor (304) is installed inside the sample container (301), and the liquid level sensor (304) is electrically connected to the control system (9).

10. The fecal microbiota transplantation bacterial solution separation device according to claim 1, characterized in that, A visual observation window (102) is provided on the housing (1); According to claim 1, the fecal microbiota transplantation bacterial liquid separation device is characterized in that it further includes a deodorization unit (10), which is connected to the accommodating space (101) and has an activated carbon adsorption layer (1001) inside.

11. The fecal microbiota transplantation bacterial solution separation device according to claim 1, characterized in that, An electromagnetic locking mechanism (201) is provided on the sealing cover (2), and the electromagnetic locking mechanism (201) is electrically connected to the control system (9).

12. The fecal microbiota transplantation bacterial solution separation device according to claim 1, characterized in that, The gas delivery pipeline (402) is equipped with a gas filter (405) and a gas flow regulating valve (406).

13. The fecal microbiota transplantation bacterial solution separation device according to claim 1, characterized in that, The bacterial liquid collection system (6) also includes a bacterial liquid dispensing unit connected to the collection container (601).

14. The fecal microbiota transplantation bacterial solution separation device according to claim 1, characterized in that, The bacterial concentration detection sensor and pH detection sensor are installed on the pipeline of the bacterial liquid collection system (6).

15. The fecal microbiota transplantation bacterial solution separation device according to claim 1, characterized in that, It also includes a waste treatment unit located at the bottom of the housing (1), which is connected to the multi-stage gradient filtration system (5) and the sample container (301).

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