Antibody acid treatment equipment

Abstract

The utility model provides antibody acid treatment equipment and relates to the field of medical instruments. Comprising a reaction tank, a stirring mechanism is arranged in the reaction tank, a low-temperature heat preservation mechanism is arranged in the reaction tank, the low-temperature heat preservation mechanism comprises an outer interlayer, a middle interlayer and an inner interlayer which are fixedly connected to the inner side of the reaction tank, the middle interlayer is arranged between the outer interlayer and the inner interlayer, and the outer interlayer and the middle interlayer are both cylindrical shells with upper and lower openings; the inner interlayer is a cylindrical shell with an opening in the bottom, a bottom sealing ring is fixedly connected between the inner interlayer and the bottom of the middle interlayer, a vacuum interlayer is formed between the outer interlayer and the side wall of the reaction tank, and a cooling water pipe is arranged between the middle interlayer and the inner interlayer. Through the arrangement of the low-temperature heat preservation mechanism, an antibody solution can be cooled and subjected to heat preservation in the acid treatment process, so that the antibody solution is always kept in a low-temperature state, and the effect of an acid treatment result is ensured.

Description

Technical Field

[0001] The utility model provides antibody acid treatment equipment, which relates to the field of medical appliances. Background Art

[0002] Acid treatment of antibodies is performed to achieve specific research or experimental purposes. Acid treatment can change the structure and properties of antibodies, and can bring advantages in cleaving antibody-antigen binding, modifying antibody surfaces, activating or deviating antibodies. When an antibody is acid treated, the following steps are generally included: selecting an appropriate acid, preparing an acid treatment solution, adding the antibody solution to be treated to the acid treatment solution, terminating the acid treatment, washing and storing. In the above process, before acid treatment, a preliminary optimization experiment should be conducted to determine the best choice of acid treatment conditions. The acid treatment time can last from a few minutes to a few hours, and the temperature needs to be carried out in a low environment.

[0003] Existing acid treatment equipment, such as the patent with publication number CN218339776U, discloses an antibody acid treatment equipment, including a reaction tank and a top cover. The reaction tank is made of glass, and the inner surface is covered with cycloolefin polymer to reduce protein adsorption. The interior of the reaction tank is equipped with a thermometer, an acidity meter, an agitator, etc., which has the characteristics of less drug adsorption loss and process automation, and can improve the efficiency of the acid treatment process. The reaction tank of this patent has a simple structure and does not have the function of low-temperature insulation. Since the acid treatment may last for a long time and the subsequent standing time is also relatively long, the reaction tank is affected by the ambient temperature, resulting in a large temperature fluctuation range or the inability to always maintain a low temperature, thereby affecting the results of the acid treatment. For this reason, we propose an antibody acid treatment equipment. Utility Model Content

[0004] The technical problem to be solved by the utility model is that the existing acid treatment reaction tank does not have the function of low-temperature heat preservation.

[0005] In order to solve the above technical problems, the technical solution provided by the utility model is: an antibody acid treatment device, comprising a reaction tank, a stirring mechanism is arranged in the reaction tank, a low-temperature insulation mechanism is arranged in the reaction tank, the low-temperature insulation mechanism comprises an outer interlayer, a middle interlayer and an inner interlayer fixedly connected to the inner side of the reaction tank, the middle interlayer is arranged between the outer interlayer and the inner interlayer, the outer interlayer and the middle interlayer are both cylindrical shells with upper and lower openings, the inner interlayer is a cylindrical shell with a bottom opening, and a bottom sealing ring is fixedly connected between the bottom of the inner interlayer and the middle interlayer, a vacuum barrier is formed between the outer interlayer and the side wall of the reaction tank, and a cooling water pipe is arranged between the middle interlayer and the inner interlayer.

[0006] Preferably, the cooling water pipe includes an outer circulation pipe spirally wound around the outer wall of the middle interlayer, a bottom circulation pipe fixed to the bottom of the bottom sealing ring, and an inner circulation pipe spirally wound around the inner wall of the inner interlayer, the bottom circulation pipe is connected between the outer circulation pipe and the inner circulation pipe, one end of the outer circulation pipe away from the bottom circulation pipe is connected to a water inlet pipe, and one end of the inner circulation pipe away from the bottom circulation pipe is connected to a water outlet pipe, and the water inlet pipe and the water outlet pipe both extend to the outside of the reaction tank.

[0007] Preferably, the stirring mechanism includes a motor fixedly connected to the top of the reaction tank, a rotating shaft fixedly connected to the output shaft of the motor, and a stirring blade fixedly connected to the rotating shaft, and the rotating shaft is Z-shaped.

[0008] Preferably, the top of the reaction tank is provided with an antibody solution inlet, an acid solution inlet pipe and an alkali solution inlet pipe, and the bottom of the reaction tank is provided with a liquid outlet pipe.

[0009] The beneficial effects of the utility model are as follows: through the arrangement of the low-temperature heat preservation mechanism, the antibody solution can be cooled and kept warm during the acid treatment process, so that the antibody solution is always kept at a low temperature, thereby ensuring that the result of the acid treatment is effective. BRIEF DESCRIPTION OF THE DRAWINGS

[0010] Figure 1 The utility model is a schematic diagram of the overall structure of an antibody acid treatment device.

[0011] Figure 2 It is a half-section diagram of an antibody acid treatment device of the utility model.

[0012] Figure 3 The utility model is a schematic diagram of the cooling water pipe structure of an antibody acid treatment device.

[0013] Figure 4 This is a half-section diagram of a reaction tank without a cooling water pipe of an antibody acid treatment device of the utility model.

[0014] (1. Reaction tank; 2. Antibody solution inlet; 3. Acid solution inlet pipe; 4. Motor; 5. Alkaline solution inlet pipe; 6. Water inlet pipe; 7. Water outlet pipe; 8. Rotating shaft; 9. External circulation pipe; 10. Bottom circulation pipe; 11. Internal circulation pipe; 12. Vacuum interlayer; 13. Inner interlayer; 14. Outer interlayer; 15. Stirring blade; 16. Bottom sealing ring; 17. Middle interlayer) DETAILED DESCRIPTION

[0015] The preferred embodiments of the present utility model will be described in detail below in conjunction with the accompanying drawings.

[0016] Reference Figures 1 to 4The utility model provides an antibody acid treatment device, including a reaction tank 1, wherein a stirring mechanism is arranged in the reaction tank 1, and a low-temperature heat preservation mechanism is arranged in the reaction tank 1, wherein the low-temperature heat preservation mechanism includes an outer interlayer 14, a middle interlayer 17 and an inner interlayer 13 fixedly connected to the inner side of the reaction tank 1, wherein the middle interlayer 17 is arranged between the outer interlayer 14 and the inner interlayer 13, wherein the outer interlayer 14 and the middle interlayer 17 are both cylindrical shells with upper and lower openings, wherein the inner interlayer 13 is a cylindrical shell with a bottom opening, and a bottom sealing ring 16 is fixedly connected between the bottom of the inner interlayer 13 and the middle interlayer 17, wherein a vacuum interlayer 12 is formed between the outer interlayer 14 and the side wall of the reaction tank 1, and a cooling water pipe is arranged between the middle interlayer 17 and the inner interlayer 13. Figure 2 , Figure 4 As shown, the inner interlayer 13 and the middle interlayer 17 cooperate with each other to form an annular liquid storage space in the reaction tank 1, and then a cooling water pipe is arranged between the middle interlayer 17 and the inner interlayer 13 to cool the antibody solution, and the vacuum interlayer 12 has a heat preservation effect, so that the antibody solution is kept at a low temperature.

[0017] Furthermore, the cooling water pipe in the utility model includes an outer circulation pipe 9 spirally wound around the outer wall of the middle interlayer 17, a bottom circulation pipe 10 fixed to the bottom of the bottom sealing ring 16, and an inner circulation pipe 11 spirally wound around the inner wall of the inner interlayer 13, the bottom circulation pipe 10 is connected between the outer circulation pipe 9 and the inner circulation pipe 11, the end of the outer circulation pipe 9 away from the bottom circulation pipe 10 is connected to the water inlet pipe 6, the end of the inner circulation pipe 11 away from the bottom circulation pipe 10 is connected to the water outlet pipe 7, and the water inlet pipe 6 and the water outlet pipe 7 both extend to the outside of the reaction tank 1. Specifically, as Figure 2 , Figure 3 As shown, the cooling water pipes are all in a threaded shape, which can increase the contact area with the inner interlayer 13 and the middle interlayer 17 and improve the cooling efficiency. Furthermore, an external cooling water device, such as a water chiller, is connected through an inlet pipe 6 and an outlet pipe 7. The inlet pipe 6 is connected to the water outlet of the water chiller, and the outlet pipe 7 is connected to the water inlet of the water chiller. Cold water can be transported to the cooling water pipes to form a circulation. The water chiller is a prior art, so it is not described in detail.

[0018] Furthermore, the stirring mechanism in the utility model includes a motor 4 fixedly connected to the top of the reaction tank 1, a rotating shaft 8 fixedly connected to the output shaft of the motor 4, and a stirring blade 15 fixedly connected to the rotating shaft 8, and the rotating shaft 8 is Z-shaped. Specifically, the motor 4 can drive the rotating shaft 8 to rotate around the inner interlayer 13 to stir the antibody solution.

[0019] Furthermore, the top of the reaction tank 1 in the utility model is provided with an antibody solution inlet 2, an acid solution inlet pipe 3 and an alkali solution inlet pipe 5, and a liquid outlet pipe is provided at the bottom of the reaction tank 1. Specifically, the antibody solution inlet 2 is used to inject the antibody solution into the reaction tank 1, the acid solution inlet pipe 3 is used to inject the acid solution for acid treatment into the reaction tank 1, and the alkali solution inlet pipe 5 is used to inject the alkaline solution into the reaction tank 1 to neutralize the excess acid solution.

[0020] Working principle: When acid treatment is carried out, the water inlet pipe 6 and the water outlet pipe 7 are connected to the external cooling water device, and cooling water is transported to the water inlet pipe 6. The cooling water flows into the outer circulation pipe 9, the bottom circulation pipe 10 and the inner circulation pipe 11 in sequence, and then flows back to the cooling water device from the water outlet pipe 7 to form a cycle. The internal antibody solution is cooled by the cooling water. The outer circulation pipe 9, the bottom circulation pipe 10 and the inner circulation pipe 11 cool the antibody solution from the inner and outer sides and the bottom respectively, and the cooling effect is better and more uniform. The vacuum barrier 12 has a heat preservation effect, so that during the acid treatment process, the antibody solution is always in a low temperature state to ensure the effect of the acid treatment.

[0021] The above description of the utility model and its implementation methods is not restrictive. The drawings show only one implementation method of the utility model, and the actual structure is not limited thereto. In short, if ordinary technicians in this field are inspired by it and design structural methods and embodiments similar to the technical solution without creativity without departing from the purpose of the invention of the utility model, they should all fall within the protection scope of the utility model.

Claims

1. An antibody acid treatment device, comprising a reaction tank, wherein a stirring mechanism is provided in the reaction tank, characterized in that: A low-temperature heat preservation mechanism is provided in the reaction tank, and the low-temperature heat preservation mechanism includes an outer interlayer, a middle interlayer and an inner interlayer fixedly connected to the inner side of the reaction tank, the middle interlayer is arranged between the outer interlayer and the inner interlayer, the outer interlayer and the middle interlayer are both cylindrical shells with upper and lower openings, the inner interlayer is a cylindrical shell with a bottom opening, and a bottom sealing ring is fixedly connected between the bottoms of the inner interlayer and the middle interlayer, a vacuum barrier is formed between the outer interlayer and the side wall of the reaction tank, and a cooling water pipe is arranged between the middle interlayer and the inner interlayer.

2. The antibody acid treatment device according to claim 1, characterized in that: The cooling water pipe includes an outer circulation pipe spirally wound around the outer wall of the middle interlayer, a bottom circulation pipe fixed to the bottom of the bottom sealing ring, and an inner circulation pipe spirally wound around the inner wall of the inner interlayer. The bottom circulation pipe is connected between the outer circulation pipe and the inner circulation pipe. The end of the outer circulation pipe away from the bottom circulation pipe is connected to a water inlet pipe, and the end of the inner circulation pipe away from the bottom circulation pipe is connected to a water outlet pipe. The water inlet pipe and the water outlet pipe both extend to the outside of the reaction tank.

3. The antibody acid treatment device according to claim 1, characterized in that: The stirring mechanism comprises a motor fixedly connected to the top of the reaction tank, a rotating shaft fixedly connected to the output shaft of the motor, and a stirring blade fixedly connected to the rotating shaft, and the rotating shaft is Z-shaped.

4. The antibody acid treatment device according to claim 1, characterized in that: The top of the reaction tank is provided with an antibody solution inlet, an acid solution inlet pipe and an alkali solution inlet pipe, and the bottom of the reaction tank is provided with a liquid outlet pipe.

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