Liquid inlet flow path switching device of single-pump gradient chromatography system

Abstract

The utility model discloses a liquid inlet flow path switching device of a single-pump gradient chromatography system, and relates to the technical field of flow path switching. The liquid inlet flow path switching device of the single-pump gradient chromatography system comprises a valve plate, a plurality of first liquid inlet valves and a second liquid inlet valve, and each first liquid inlet valve is provided with a plurality of first inlets and a first outlet; the second liquid inlet valve is provided with a plurality of second inlets and second outlets; the plurality of first inlets are communicated with first pipelines; the plurality of second inlets are communicated with second pipelines; and the second outlet is communicated with a third pipeline. According to the liquid inlet flow path switching device, the multiple first liquid inlet valves and the multiple second liquid inlet valves are matched with one another, two-stage flow path switching is achieved, the liquid inlet efficiency is effectively improved, two plunger pumps do not need to be used, and cost is greatly saved; and meanwhile, the multiple first liquid inlet valves and the multiple second liquid inlet valves are integrated on the valve plate, and the structural compactness of the liquid inlet flow path switching device is improved.

Description

Technical Field

[0001] The present application relates to the technical field of flow path switching, and in particular to a liquid inlet flow path switching device for a single-pump gradient chromatography system. Background Art

[0002] Existing gradient chromatography systems generally include a single-pump gradient chromatography system and a dual-pump gradient chromatography system, and both gradient chromatography systems are used for gradient chromatography in the protein purification process.

[0003] However, the above two gradient chromatography systems have the following defects respectively:

[0004] like Figure 1 As shown, a conventional single-pump gradient chromatography system is shown, which includes a three-way valve 200 and a plunger pump 203. The two inlets of the three-way valve 200 are connected to the inlet pipeline 201, and the outlet is connected to the outlet pipeline 202. When liquid is introduced, the A1 bottle is first connected to one of the inlet pipelines 201, and the liquid is added to the A1 bottle for balance, and then the A1 bottle is manually replaced with the A2 bottle, and the sample is injected through the liquid added to the A2 bottle, and then the A2 bottle is manually replaced with the A1 bottle, and the B1 bottle is connected to the other inlet pipeline 201, and the liquid is added to the A1 bottle and the B1 bottle together for gradient elution, and finally the B1 bottle is manually replaced with the B2 bottle, and the liquid is added to the B2 bottle for in-situ column cleaning. As can be seen from the above content, the single-pump gradient chromatography system requires the staff to frequently replace the liquid inlet bottle during the chromatography process, which is not only time-consuming and labor-intensive, but also has low liquid introduction efficiency.

[0005] like Figure 2 , which shows a conventional dual-pump gradient chromatography system, which includes two three-way valves 200 and two plunger pumps 203. The two plunger pumps 203 respectively control the liquid inlet of the two three-way valves 200. The four inlets of the three-way valve 200 are connected to the inlet pipeline 201, and the two outlets are connected to the outlet pipeline 202. Bottle A1 and bottle A2 are respectively connected to the two inlet pipelines 201 on the first three-way valve 200, and bottle B1 and bottle B2 are respectively connected to the two inlet pipelines 201 on the second three-way valve 200. On the line 201, when liquid is introduced, the first plunger pump 203 is used to drive the liquid on the A1 bottle for balancing, then the first three-way valve 200 is switched and the first plunger pump 203 is used to drive the liquid on the A2 bottle for injection, then the first three-way valve 200 is switched again, and the first plunger pump 203 and the second plunger pump 203 are used to drive the liquid on the A1 bottle and the B1 bottle for gradient elution, and finally the second three-way valve 200 is switched again, and the second plunger pump 203 is used to drive the liquid on the B2 bottle for in-situ column cleaning. As can be seen from the above content, although the dual-pump gradient chromatography system overcomes the problem of low liquid introduction efficiency of the single-pump gradient chromatography system, it requires the use of two plunger pumps, which undoubtedly greatly increases the cost. Utility Model Content

[0006] In order to overcome the deficiencies of the prior art, the present application provides a liquid inlet flow path switching device for a single-pump gradient chromatography system with high liquid inlet efficiency and low cost.

[0007] The present application provides a liquid inlet valve group adopting the following technical solution:

[0008] A liquid inlet flow path switching device for a single-pump gradient chromatography system, the single-pump gradient chromatography system comprising a plurality of liquid inlet bottles and a liquid inlet pump, the liquid inlet flow path switching device comprising a valve plate, a plurality of first liquid inlet valves and a second liquid inlet valve arranged on the valve plate, the first liquid inlet valve having a plurality of first inlets and a first outlet; the second liquid inlet valve having a plurality of second inlets and a second outlet;

[0009] The first inlets constituting the first liquid inlet valve are all connected with a first pipeline, and the other ends of the first pipelines are respectively connected with the corresponding liquid inlet bottles;

[0010] The plurality of second inlets constituting the second liquid inlet valve are all connected with a second pipeline, and the other ends of the plurality of second pipelines are respectively connected with the first outlets on the plurality of first liquid inlet valves;

[0011] The second outlet constituting the second liquid inlet valve is connected with a third pipeline, and the other end of the third pipeline is connected with the liquid inlet pump.

[0012] By adopting the above technical solution, multiple first liquid inlet valves and second liquid inlet valves cooperate with each other and realize secondary flow path switching, which not only effectively improves the liquid inlet efficiency, but also eliminates the need to use two plunger pumps, greatly saving costs; at the same time, multiple first liquid inlet valves and second liquid inlet valves are integrated on the valve plate, thereby improving the structural compactness of the liquid inlet flow path switching device.

[0013] In a specific implementation scheme, the first liquid inlet valve and the second liquid inlet valve are both three-way valves, the first liquid inlet valve has two first inlets and a first outlet, the second liquid inlet valve has two second inlets and a second outlet, and accordingly, there are four first pipelines and two second pipelines.

[0014] In a specific implementation scheme, there are two first liquid inlet valves, each of which is provided with two first inlet pipes and a first outlet pipe, the first inlet pipe end is provided with the first inlet, the first outlet pipe end is provided with the first outlet, and the distance between the two first outlet pipes constituting the two first liquid inlet valves gradually increases in the direction approaching the second liquid inlet valve.

[0015] By adopting the above technical solution, there can be a sufficient distance between the ends of the two first outlet pipes close to the second liquid inlet valve, which is not only convenient for connecting the second pipeline, but also can avoid interference between multiple second pipelines, thereby improving the injection efficiency.

[0016] In a specific implementation manner, the two first liquid inlet valves are symmetrically arranged with respect to the second liquid inlet valve.

[0017] By adopting the above technical solution, the liquid delivered by the two first liquid inlet valves can be respectively input into the second liquid inlet valve from both sides of the second liquid inlet valve, effectively avoiding interference between the two first liquid inlet valves and greatly improving the liquid inlet efficiency.

[0018] In a specific implementation manner, the two first inlet pipes are inlet pipe a and inlet pipe b respectively, and the inlet pipe a and the inlet pipe b are arranged at an angle.

[0019] By adopting the above technical solution, it is not only convenient to connect the first pipeline, but also can effectively avoid interference between the first pipeline connected to the inlet pipe a and the first pipeline connected to the inlet pipe b, thereby improving the injection efficiency.

[0020] In a specific feasible implementation scheme, the two inlet pipes a are arranged adjacent to each other, and the distance between the two inlet pipes a gradually decreases in the direction away from the second liquid inlet valve; the two inlet pipes b are respectively located on the side away from each other of the two inlet pipes a, and the distance between the two inlet pipes b gradually increases in the direction away from the second liquid inlet valve.

[0021] By adopting the above technical solution, the two inlet pipes a and the two inlet pipes b can be directed in different directions respectively, effectively avoiding interference between the first pipeline connected to the inlet pipe a and the first pipeline connected to the inlet pipe b, thereby improving the injection efficiency.

[0022] In a specific possible implementation scheme, the second liquid inlet valve is provided with two second inlet pipes and a second outlet pipe, the second inlet pipe end is provided with the second inlet, the second outlet pipe end is provided with the second outlet, the two second inlet pipes are arranged back to back and extend in directions away from each other, and the end of the second inlet pipe in the extension direction is close to the corresponding end of the first outlet pipe in the extension direction.

[0023] By adopting the above technical solution, it is not only convenient to connect the second pipeline, but also the second inlet pipe and the first outlet pipe close to each other can shorten the flow distance of the liquid in the second pipeline, thereby improving the liquid inlet efficiency and liquid inlet stability.

[0024] In a specific feasible implementation scheme, the multiple first liquid inlet valves and the second liquid inlet valves are respectively inserted on the valve plate, the first liquid inlet valve includes a first valve body and a first valve seat coaxially connected, the second liquid inlet valve includes a second valve body and a second valve seat coaxially connected, the first valve body and the second valve body are located on one side of the valve plate in the thickness direction, and the first valve seat and the second valve seat are located on the other side of the valve plate in the thickness direction, and the liquid inlet flow path switching device also includes a plurality of fixing rings respectively correspondingly mounted on the multiple first valve seats and the second valve seats, and the fixing rings are connected to the valve plate.

[0025] By adopting the above technical solution, the connection strength between the first liquid inlet valve, the second liquid inlet valve and the valve plate is effectively improved.

[0026] In a specific possible implementation scheme, the valve plate includes a plate body and a first fixed plate attached to one side of the plate body in the thickness direction, the first valve seat and the second valve seat are respectively penetrated through the first fixed plate, and the fixed ring abuts against the first fixed plate.

[0027] By adopting the above technical solution, the connection strength between the first liquid inlet valve and the second liquid inlet valve and the valve plate is further improved.

[0028] In a specific implementation scheme, a fixing groove is formed on one side of the plate body away from the first fixing plate, and the valve plate also includes a second fixing plate embedded in the fixing groove, and the first valve body and the second valve body are respectively inserted into the second fixing plate.

[0029] By adopting the above technical solution, the connection strength between the first liquid inlet valve and the second liquid inlet valve and the valve plate is further improved.

[0030] In summary, the present application includes at least one of the following beneficial technical effects:

[0031] (1) Multiple first liquid inlet valves and second liquid inlet valves cooperate with each other and realize secondary flow path switching, which effectively improves the liquid inlet efficiency;

[0032] (2) No need to use two plunger pumps, which greatly saves costs;

[0033] (3) A plurality of first liquid inlet valves and second liquid inlet valves are integrated on the valve plate, thereby improving the structural compactness of the liquid inlet flow path switching device. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] Figure 1 It is a schematic diagram of the flow path of a single-pump chromatography system in the prior art;

[0035] Figure 2 It is a schematic diagram of the flow path of a dual-pump chromatography system in the prior art;

[0036] Figure 3 is a schematic diagram of the flow path of a single-pump gradient chromatography system using the inlet flow path switching device of this embodiment;

[0037] Figure 4 is a schematic diagram of the three-dimensional structure of the liquid inlet flow path switching device of an embodiment of the present application;

[0038] Figure 5 yes Figure 4 Rear view of.

[0039] Description of reference numerals:

[0040] 1. Valve plate; 11. Plate body; 12. First fixed plate; 13. Fixed groove; 14. Second fixed plate; 2. First liquid inlet valve; 21. First inlet; 22. First outlet; 23. First inlet pipe; 231. Inlet pipe a; 232. Inlet pipe b; 24. First outlet pipe; 25. First valve body; 26. First valve seat; 3. Second liquid inlet valve; 31. Second inlet; 32. Second outlet; 33. Second inlet pipe; 34. Second outlet pipe; 35. Second valve body; 36. Second valve seat; 4. First pipeline; 5. Second pipeline; 6. Third pipeline; 7. Fixed ring;

[0041] 100. Liquid inlet bottle; 101. Liquid inlet pump;

[0042] 200, three-way valve; 201, inlet pipeline; 202, outlet pipeline; 203, plunger pump. DETAILED DESCRIPTION

[0043] The present application is further described in detail below in conjunction with the accompanying drawings.

[0044] See also Figure 3-5 As shown, a single-pump gradient chromatography system is shown, including four liquid inlet bottles 100, a liquid inlet pump 101 and a liquid inlet flow path switching device, the liquid inlet flow path switching device includes a valve plate 1, two first liquid inlet valves 2 and a second liquid inlet valve 3 arranged on the valve plate 1, the first liquid inlet valve 2 and the second liquid inlet valve 3 are both three-way valves, the first liquid inlet valve 2 has two first inlets 21 and a first outlet 22, and the second liquid inlet valve 3 has two second inlets 31 and a second outlet 32;

[0045] The two first inlets 21 constituting the first liquid inlet valve 2 are both connected with the first pipeline 4, and the other ends of the two first pipelines 4 are respectively connected with the two liquid inlet bottles 100;

[0046] The two second inlets 31 constituting the second liquid inlet valve 3 are both connected with the second pipeline 5, and the other ends of the two second pipelines 5 are respectively connected with the first outlets 22 on the two first liquid inlet valves 2;

[0047] The second outlet 32 ​​constituting the second liquid inlet valve 3 is connected to a third pipeline 6, and the other end of the third pipeline 6 is connected to the liquid inlet pump 101;

[0048] There are four first pipelines 4 , two second pipelines 5 , and two first liquid inlet valves 2 are symmetrically arranged with respect to the second liquid inlet valve 3 .

[0049] Through the above-mentioned arrangement, the two first liquid inlet valves 2 and the second liquid inlet valve 3 cooperate with each other and realize secondary flow path switching, which not only effectively improves the liquid inlet efficiency, but also eliminates the need to use two plunger pumps, greatly saving costs; at the same time, the two first liquid inlet valves 2 and the second liquid inlet valve 3 are integrated on the valve plate 1, thereby improving the structural compactness of the liquid inlet flow path switching device.

[0050] Specifically, Figure 3 As shown, the four liquid inlet bottles 100 are respectively A1 bottle, A2 bottle, B1 bottle and B2 bottle, the first liquid inlet valve 2 and the second liquid inlet valve 3 both have a first flow path and a second flow path, when liquid is inlet, the first flow path of the first liquid inlet valve 2 and the first flow path of the second liquid inlet valve 3 are opened first, and the liquid in the A1 bottle flows through the two first flow paths under the drive of the liquid inlet pump 101 and the liquid is inlet, and then the first flow path of the first liquid inlet valve 2 is switched to the second flow path, and the liquid in the A2 bottle flows through the second flow path of the first liquid inlet valve 2 under the drive of the liquid inlet pump 101. The first flow path of the second liquid inlet valve 3 and the first flow path of the second first liquid inlet valve 2 are opened, and then the second flow path of the second liquid inlet valve 3 and the first flow path of the second first liquid inlet valve 2 are opened, and the second flow path of the first first liquid inlet valve 2 is switched to the first flow path, and the liquid in the A1 bottle and the liquid in the B1 bottle are fed together under the drive of the liquid inlet pump 101. Finally, the first flow path of the second liquid inlet valve 3 is closed, the first first liquid inlet valve 2 is closed, and the first flow path of the second first liquid inlet valve 2 is switched to the second flow path, and the liquid in the B2 bottle is fed under the drive of the liquid inlet pump 101.

[0051] In this embodiment, combined with Figure 4 As shown, each first liquid inlet valve 2 is provided with two first inlet pipes 23 and a first outlet pipe 24, a first inlet 21 is provided at the end of the first inlet pipe 23, and a first outlet 22 is provided at the end of the first outlet pipe 24, and the distance between the two first outlet pipes 24 constituting the two first liquid inlet valves 2 gradually increases in the direction close to the second liquid inlet valve 3. In this way, there can be a sufficient distance between the ends of the two first outlet pipes 24 close to the second liquid inlet valve 3, which is not only convenient for connecting the second pipeline 5, but also can avoid interference between multiple second pipelines 5, thereby improving the injection efficiency.

[0052] In this embodiment, again combined Figure 4As shown, the two first inlet pipes 23 are respectively an inlet pipe a231 and an inlet pipe b232, and the inlet pipe a231 and the inlet pipe b232 are arranged at an angle; the two inlet pipes a231 are arranged adjacent to each other, and the distance between the two inlet pipes a231 gradually decreases in the direction away from the second liquid inlet valve 3, and the two inlet pipes b232 are respectively located on the side away from each other of the two inlet pipes a231, and the distance between the two inlet pipes b232 gradually increases in the direction away from the second liquid inlet valve 3.

[0053] Through the above arrangement, not only is it convenient to connect the first pipeline 4, but the two inlet pipes a231 and the two inlet pipes b232 can be directed in different directions respectively, which can effectively avoid interference between the first pipeline 4 connected to the inlet pipe a231 and the first pipeline 4 connected to the inlet pipe b232, thereby improving the injection efficiency.

[0054] In this embodiment, the second liquid inlet valve 3 is provided with two second inlet pipes 33 and a second outlet pipe 34. The second inlet pipe 33 is provided with a second inlet 31 at the end thereof, and the second outlet pipe 34 is provided with a second outlet 32 ​​at the end thereof. The two second inlet pipes 33 are arranged opposite to each other and extend in directions away from each other, and the end of the second inlet pipe 33 in the extending direction is close to the end of the corresponding first outlet pipe 24 in the extending direction. In this way, it is not only convenient to connect the second pipeline 5, but also the second inlet pipe 33 and the first outlet pipe 24 close to each other can shorten the flow distance of the liquid in the second pipeline 5, thereby improving the liquid inlet efficiency and liquid inlet stability.

[0055] In this embodiment, combined with Figure 5 As shown, two first liquid inlet valves 2 and second liquid inlet valves 3 are respectively inserted on the valve plate 1, the first liquid inlet valve 2 includes a first valve body 25 and a first valve seat 26 connected coaxially, the second liquid inlet valve 3 includes a second valve body 35 and a second valve seat 36 connected coaxially, the first valve body 25 and the second valve body 35 are located on one side of the thickness direction of the valve plate 1, and the first valve seat 26 and the second valve seat 36 are located on the other side of the thickness direction of the valve plate 1, and the liquid inlet flow path switching device also includes a plurality of fixing rings 7 respectively correspondingly sleeved on the plurality of first valve seats 26 and the second valve seats 36, and the fixing ring 7 is connected to the valve plate 1. The fixing ring 7 is a clamp, and its side is connected to the valve plate 1 by bolts.

[0056] The valve plate 1 includes a plate body 11 and a first fixing plate 12 attached to one side of the plate body 11 in the thickness direction. The first valve seat 26 and the second valve seat 36 are respectively inserted into the first fixing plate 12 . The fixing ring 7 abuts against the first fixing plate 12 .

[0057] A fixing groove 13 is formed on one side of the plate body 11 away from the first fixing plate 12 . The valve plate 1 further includes a second fixing plate 14 embedded in the fixing groove 13 . The first valve body 25 and the second valve body 35 are respectively inserted into the second fixing plate 14 .

[0058] In this way, through the cooperation of the first fixing plate 12 , the fixing ring 7 and the second fixing plate 14 , the connection strength between the first liquid inlet valve 2 , the second liquid inlet valve 3 and the valve plate 1 can be effectively improved.

[0059] In this embodiment, the first liquid inlet valve 2 and the second liquid inlet valve 3 are both three-way solenoid valves.

[0060] The above are all preferred embodiments of the present application, and the protection scope of the present application is not limited thereto. Therefore, any equivalent changes made according to the structure, shape, and principle of the present application should be included in the protection scope of the present application.

Claims

1. A liquid inlet flow path switching device for a single-pump gradient chromatography system, the single-pump gradient chromatography system comprising a plurality of liquid inlet bottles (100) and a liquid inlet pump (101), characterized in that: The liquid inlet flow path switching device comprises a valve plate (1), a plurality of first liquid inlet valves (2) and a second liquid inlet valve (3) arranged on the valve plate (1), wherein the first liquid inlet valve (2) has a plurality of first inlets (21) and a first outlet (22); and the second liquid inlet valve (3) has a plurality of second inlets (31) and a second outlet (32); The plurality of first inlets (21) constituting the first liquid inlet valve (2) are all connected to a first pipeline (4), and the other ends of the plurality of first pipelines (4) are respectively connected to the plurality of liquid inlet bottles (100); The plurality of second inlets (31) constituting the second liquid inlet valve (3) are all connected to a second pipeline (5), and the other ends of the plurality of second pipelines (5) are respectively connected to the first outlets (22) on the plurality of first liquid inlet valves (2); The second outlet (32) constituting the second liquid inlet valve (3) is connected to a third pipeline (6), and the other end of the third pipeline (6) is connected to the liquid inlet pump (101).

2. The liquid inlet flow path switching device of a single-pump gradient chromatography system according to claim 1, characterized in that: The first liquid inlet valve (2) and the second liquid inlet valve (3) are both three-way valves. The first liquid inlet valve (2) has two first inlets (21) and a first outlet (22), and the second liquid inlet valve (3) has two second inlets (31) and a second outlet (32). Correspondingly, there are four first pipelines (4) and two second pipelines (5).

3. The liquid inlet flow path switching device of a single-pump gradient chromatography system according to claim 2, characterized in that: There are two first liquid inlet valves (2), and each of the first liquid inlet valves (2) is provided with two first inlet pipes (23) and a first outlet pipe (24). The first inlet pipe (23) is provided with the first inlet (21) at the end thereof, and the first outlet pipe (24) is provided with the first outlet (22) at the end thereof. The distance between the two first outlet pipes (24) constituting the two first liquid inlet valves (2) gradually increases in a direction approaching the second liquid inlet valve (3).

4. The liquid inlet flow path switching device of a single-pump gradient chromatography system according to claim 3, characterized in that: The two first liquid inlet valves (2) are arranged symmetrically with respect to the second liquid inlet valve (3).

5. The liquid inlet flow path switching device of a single-pump gradient chromatography system according to claim 3, characterized in that: The two first inlet pipes (23) are respectively inlet pipe a (231) and inlet pipe b (232), and the inlet pipe a (231) and the inlet pipe b (232) are arranged at an angle.

6. The liquid inlet flow path switching device of a single-pump gradient chromatography system according to claim 5, characterized in that: The two inlet pipes a (231) are arranged adjacent to each other, and the distance between the two inlet pipes a (231) gradually decreases in a direction away from the second liquid inlet valve (3); the two inlet pipes b (232) are respectively located on a side away from the two inlet pipes a (231), and the distance between the two inlet pipes b (232) gradually increases in a direction away from the second liquid inlet valve (3).

7. The liquid inlet flow path switching device of a single-pump gradient chromatography system according to claim 3, characterized in that: The second liquid inlet valve (3) is provided with two second inlet pipes (33) and a second outlet pipe (34); the second inlet pipe (33) is provided with the second inlet (31) at the end thereof, and the second outlet pipe (34) is provided with the second outlet (32) at the end thereof; the two second inlet pipes (33) are arranged opposite to each other and extend in directions away from each other, and the end of the second inlet pipe (33) in the extension direction is close to the end of the corresponding first outlet pipe (24) in the extension direction.

8. The liquid inlet flow path switching device of a single-pump gradient chromatography system according to claim 1 or 2, characterized in that: The plurality of first liquid inlet valves (2) and the second liquid inlet valves (3) are respectively inserted on the valve plate (1), the first liquid inlet valve (2) comprises a first valve body (25) and a first valve seat (26) which are coaxially connected, the second liquid inlet valve (3) comprises a second valve body (35) and a second valve seat (36) which are coaxially connected, the first valve body (25) and the second valve body (35) are located on one side of the thickness direction of the valve plate (1), the first valve seat (26) and the second valve seat (36) are located on the other side of the thickness direction of the valve plate (1), the liquid inlet flow path switching device also comprises a plurality of fixing rings (7) which are respectively correspondingly sleeved on the plurality of first valve seats (26) and the second valve seat (36), and the fixing rings (7) are connected to the valve plate (1).

9. The liquid inlet flow path switching device of a single-pump gradient chromatography system according to claim 8, characterized in that: The valve plate (1) comprises a plate body (11), a first fixed plate (12) attached to one side of the plate body (11) in the thickness direction, the first valve seat (26) and the second valve seat (36) are respectively penetrated through the first fixed plate (12), and the fixed ring (7) is in contact with the first fixed plate (12).

10. The liquid inlet flow path switching device of a single-pump gradient chromatography system according to claim 9, characterized in that: A fixing groove (13) is provided on one side of the plate body (11) away from the first fixing plate (12); the valve plate (1) also includes a second fixing plate (14) embedded in the fixing groove (13); the first valve body (25) and the second valve body (35) are respectively inserted into the second fixing plate (14).

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