Device for detecting social deviation behavior of adult zebra fish

Abstract

A device for detecting the social deviation behavior of adult zebra fish comprises an outer frame, transparent glass partition plates, baffles and ground glass partition plates, the outer frame is made of ground glass, the upper end of the outer frame is open, the two transparent glass partition plates are fixedly arranged in the outer frame, the inner space of the outer frame is sequentially divided into three areas, and the baffles are arranged in the three areas. Vertical long-strip-shaped baffles are arranged at the positions, close to the two ends of the transparent glass partition plate, of the middle area, the baffles are made of transparent glass materials, the baffles are fixed to the outer frame, sliding grooves are formed between the baffles and the transparent glass partition plate, the ground glass partition plate is movably arranged in the sliding grooves, and the transparent glass partition plate can guarantee that zebra fishes in different areas can be seen mutually. Meanwhile, hormone secreted by the fish is prevented from being sensed by the opposite side to affect the experiment result, the frosted glass partition plate can ensure that zebra fishes in all areas are invisible to one another during experiment, whether the detected fish has social obstacles or not is judged by comparing two groups of detection numbers before and after frosted glass is used, the design is reasonable, experiment observation is convenient, and the detection result is accurate.

Description

Technical Field

[0001] The utility model relates to the technical field of zebrafish experimental equipment, in particular to a device for detecting social bias behavior of adult zebrafish. Background Art

[0002] Social behavior is defined as the interaction between individuals of the same species. Zebrafish is a highly social species that is prone to a range of social behaviors, such as mirror response, black and white exploration, and social behavior. Social preference is also a common social behavior of zebrafish, which is manifested in that zebrafish like to spend more time close to the same species. This is also a common phenomenon in social animals. When detecting the social preference behavior of adult zebrafish, it is necessary to design a reasonable detection device. Utility Model Content

[0003] In order to solve the problems mentioned in the above background technology, the utility model provides a device for detecting social preference behavior of adult zebrafish.

[0004] The utility model discloses the following technical scheme: a device for detecting social bias behavior of adult zebrafish, comprising an outer frame, a transparent glass partition, a baffle and a frosted glass partition, wherein the outer frame is made of frosted glass and has an upper end opening, two transparent glass partitions are fixedly arranged inside the outer frame, and the inner space of the outer frame is divided into three areas in sequence, wherein the middle area is a detection area, and vertical long strip baffles are arranged near both ends of the transparent glass partition in the detection area, wherein the baffles are made of transparent glass, and the baffles are fixed between the inner wall of the outer frame and the transparent glass partition to form a slide groove, and the frosted glass partition is movably arranged in the slide groove.

[0005] Furthermore, the dimensions of the outer frame are: 50 cm in length, 25 cm in width, and 20 cm in height.

[0006] Furthermore, the distance between the transparent glass partition and both ends of the outer frame is 10 cm.

[0007] Furthermore, the frosted glass partition is taller than the transparent glass partition.

[0008] Furthermore, a camera is provided at 40 cm above the outer frame.

[0009] Beneficial effects: Compared with the prior art, the device for detecting social preference behavior of adult zebrafish of the utility model is reasonably designed, convenient for experimental observation, and has accurate detection results. BRIEF DESCRIPTION OF THE DRAWINGS

[0010] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0011] Figure 2 A schematic diagram for removing the frosted glass partition;

[0012] In the figure: 1-outer frame, 2-transparent glass partition, 3-baffle, 4-frosted glass partition, 5-camera. DETAILED DESCRIPTION

[0013] like Figure 1 to Figure 2 As shown, a device for detecting social preference behavior of adult zebrafish includes an outer frame 1, a transparent glass partition 2, a baffle 3 and a frosted glass partition 4. The outer frame 1 is made of frosted glass and has an opening at the upper end. Two transparent glass partitions 2 are fixedly arranged inside the outer frame 1 to divide the internal space of the outer frame 1 into three areas in sequence: a similar area, a detection area and a blank area. The transparent glass partition 2 can ensure that zebrafish in different areas can see each other. The areas are not connected to each other, which can prevent the hormones secreted by fish from being felt by the other party through water and affecting the experimental results. The middle area is the detection area. Vertical long strip baffles 3 are arranged near both ends of the transparent glass partition 2 in the detection area. The baffle 3 is made of transparent glass. The baffle 3 is fixed between the inner wall of the outer frame 1 and the transparent glass partition 2 to form a slide groove. The frosted glass partition 4 is movably arranged in the slide groove, which can ensure that the zebrafish in different areas are invisible to each other during the experiment.

[0014] The dimensions of the outer frame 1 are: 50 cm in length, 25 cm in width, and 20 cm in height, which are convenient for the movement of zebrafish and the observation of their behaviors; the distance between the transparent glass partition 2 and both ends of the outer frame 1 is 10 cm, which ensures that the size of the detection space is reasonable; the height of the frosted glass partition 4 is greater than that of the transparent glass partition 2, which is convenient for taking the frosted glass partition 4; a camera 5 is also provided 40 cm above the upper part of the outer frame 1, which is connected to a video storage device to record the behavior of zebrafish and ensure the accuracy of the detection data.

[0015] During the experiment, the indoor temperature was maintained at 28°C, the light was constant, and the room was quiet. The total experimental time was 10 min, with 6 min of adaptation and 4 min of detection. First, aquaculture water was added to the homogeneous area, the detection area and the blank area, and the aquaculture water level of the three areas was kept at the same height of 12 cm. At this time, the frosted glass partition 4 was placed in the slide to ensure that the three areas were opaque. The zebrafish to be tested was placed in the detection area, and three zebrafish were placed in the homogeneous area. The zebrafish in the detection area and the homogeneous area were all sexually mature zebrafish over 3 months old. The detection area was divided into a proximal area, an intermediate area and a distal area from the homogeneous area to the blank area, and the percentage of time that the zebrafish stayed in the proximal detection area close to the homogeneous area accounted for the total detection area activity; after the detection, the frosted glass partitions 4 on both sides were removed and tested again to compare whether the tested fish spent more time in the proximal area. If the time spent cruising in the proximal area was longer, it means that the fish has no social disorder; in order to prevent the tested fish from having a cruising habit, the blank area and the homogeneous area can be swapped and tested again to ensure the accuracy of the experimental results.

[0016] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes within the technical scope disclosed by the present invention according to the technical scheme and the utility model concept of the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A device for detecting socially biased behavior of adult zebrafish, comprising an outer frame (1), a transparent glass partition (2), a baffle (3) and a frosted glass partition (4), characterized in that: The outer frame (1) is made of frosted glass and has an opening at the top. Two transparent glass partitions (2) are fixedly arranged inside the outer frame (1) to divide the internal space of the outer frame (1) into three areas in sequence. A vertical long strip baffle (3) is arranged in the middle area near both ends of the transparent glass partition (2). The baffle (3) is made of transparent glass. The baffle (3) is fixed between the inner wall of the outer frame (1) and the transparent glass partition (2) to form a slide groove. The frosted glass partition (4) is movably arranged in the slide groove.

2. The device for detecting social preference behavior of adult zebrafish according to claim 1, characterized in that: The dimensions of the outer frame (1) are: 50 cm in length, 25 cm in width, and 20 cm in height.

3. The device for detecting social preference behavior of adult zebrafish according to claim 1, characterized in that: The distance between the two ends of the transparent glass partition (2) and the outer frame (1) is 10 cm.

4. The device for detecting social preference behavior of adult zebrafish according to claim 1, characterized in that: The frosted glass partition (4) is taller than the transparent glass partition (2).

5. The device for detecting social preference behavior of adult zebrafish according to claim 1, characterized in that: A camera (5) is also provided 40 cm above the outer frame (1).

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