Skin treatment device

Abstract

The skin treatment device is suitable for effectively inhibiting melanin from being generated on the irradiated skin part of a person. The skin treatment device comprises a light source which is used for generating specified light with a central wavelength in a wavelength range which is longer than 490nm and smaller than 525nm, and the specified light from the light source can be irradiated to the skin. The prescribed light preferably has a central wavelength of approximately 505 nm.

Description

Technical Field

[0001] The utility model relates to a skin treatment device. Background Art

[0002] There is known a skin wound healing device including an ultra-narrow-band light irradiation mechanism that generates ultra-narrow-band green light having a peak wavelength of 500 nm to 540 nm and a half-peak width of 10 nm or less.

[0003] Prior art literature

[0004] Patent Literature

[0005] Patent Document 1: WO2011 / 067941 Specification Utility Model Content

[0006] Issues to be solved by utility models

[0007] However, in the above-mentioned conventional techniques, it is difficult to effectively suppress the production of melanin in the skin area of ​​the irradiated person.

[0008] Therefore, an object of the present invention is to provide a skin treatment device that can effectively suppress the production of melanin in the skin area of ​​a person being irradiated.

[0009] Means for solving problems

[0010] The first technical solution of the utility model is a skin treatment device, which includes a light source that generates specified light with a central wavelength within a wavelength range longer than 490nm and less than 525nm, and can irradiate the skin with the specified light from the light source, wherein the half-maximum half-width of the specified light is less than ±20nm, and the specified light is light emitted by an LED light source.

[0011] Furthermore, in the above-mentioned skin treatment device, the predetermined light has a central wavelength of approximately 505 nm.

[0012] In addition, in the above skin treatment device, at 0.5 mW / cm 2 Above and 62mW / cm 2 The prescribed light is irradiated at the following radiation intensity.

[0013] In addition, in the above skin treatment device, the radiation intensity is 11.5 mW / cm 2 Above and 62mW / cm 2 the following.

[0014] In addition, in the above skin treatment device, at 0.09 J / cm 2 Above and 45J / cm 2The prescribed light is irradiated with an irradiation energy in the following range.

[0015] In addition, in the above-mentioned skin treatment device, the irradiation energy is 0.09 J / cm 2 Above and 11J / cm 2 the following.

[0016] Furthermore, in the above-mentioned skin treatment device, the light source includes an LED element.

[0017] Furthermore, in the above-mentioned skin treatment device, a plurality of the LED elements are mounted on one chip.

[0018] Furthermore, in the above-mentioned skin treatment device, the light source is provided so that the predetermined light can be irradiated in a manner that suppresses the generation of melanin in a skin area of ​​a person being irradiated, compared with light having a central wavelength outside the wavelength range.

[0019] Furthermore, in the above-mentioned skin treatment device, the light source is arranged so that the prescribed light can be irradiated in such a manner that the irradiated skin portion of the person has an effect of suppressing the production of melanin by 5% or more compared to a case where the prescribed light is not irradiated.

[0020] In addition, the above-mentioned skin treatment device further includes a control unit, which controls the irradiation of light in one or more action modes, and the one or more action modes have a prescribed action mode that establishes a corresponding relationship with the effect of inhibiting the production of melanin, and the control unit is configured to output the prescribed light in the prescribed action mode.

[0021] Furthermore, in the above-mentioned skin treatment device, the light source is arranged so as to achieve continuous light irradiation for more than 1 minute, with the ratio of the irradiation time of the predetermined light being more than 1 / 2.

[0022] Furthermore, in the above-mentioned skin treatment device, the light source is configured such that the predetermined light from the light source can promote the expression of keratin 10 .

[0023] Furthermore, in the above-mentioned skin treatment device, the light source is configured to be controlled so that, in an operation mode of the skin treatment device for irradiating a wavelength range longer than 490 nm and less than 525 nm, a total irradiation time in the operation mode is at least 3 minutes.

[0024] In one embodiment, a skin treatment device is disclosed, including a light source that generates predetermined light having a central wavelength within a wavelength range longer than 490 nm and not more than 525 nm, and capable of irradiating the skin with the predetermined light from the light source.

[0025] Effect of utility model

[0026] According to the utility model, the generation of melanin in the skin area of ​​the irradiated person can be effectively suppressed. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 It is a perspective view showing the appearance of the light irradiation device according to the present embodiment.

[0028] Figure 2 It is an explanatory diagram of the characteristics of light (predetermined light) generated from an LED.

[0029] Figure 3 This is a diagram showing an overview of a control system of a light irradiation device.

[0030] Figure 3A This is a diagram showing an example of the hardware configuration of the control device.

[0031] Figure 4A This is a diagram showing the experimental results (part 1) of the effect of suppressing melanin production obtained by irradiation with green LED light.

[0032] Figure 4B This is a diagram showing the experimental results (part 2) of the effect of suppressing melanin production obtained by irradiation with green LED light.

[0033] Figure 5A This is a diagram showing the experimental results (part 3) of the effect of suppressing the production of melanin obtained by irradiation with green LED light.

[0034] Figure 5B This is a diagram showing the experimental results (part 4) of the effect of suppressing the production of melanin obtained by irradiation with green LED light.

[0035] Figure 5C This is a diagram showing the experimental results (part 5) of the effect of suppressing the production of melanin obtained by irradiation with green LED light.

[0036] Figure 5D This is a diagram showing the experimental results (part 6) of the effect of suppressing the production of melanin obtained by irradiation with green LED light.

[0037] Figure 5E Is FIG. 5A to FIG. 5E Table of evaluation results obtained from the test results.

[0038] Fig. 6A This is a diagram showing the experimental results (part 7) of the effect of suppressing the production of melanin obtained by irradiation with green LED light.

[0039] Figure 6B This is a diagram showing the experimental results (part 8) of the effect of suppressing the production of melanin obtained by irradiation with green LED light.

[0040] Fig. 7A This is a diagram showing the experimental results (part 9) of the effect of suppressing the production of melanin obtained by irradiation with green LED light.

[0041] Figure 7B This is a diagram showing the experimental results (part 10) of the effect of suppressing the production of melanin obtained by irradiation with green LED light.

[0042] Figure 8 This is a table showing the relationship between the radiation intensity of green LED light and the effect (the effect of suppressing the production of melanin).

[0043] Fig. 9 This is a graph showing the relationship between the radiation intensity of green LED light and the effect (Part 1).

[0044] Fig.10 This is a graph showing the relationship between the radiation intensity of green LED light and the effect (part 2).

[0045] Fig.11 This is a graph showing the relationship between the radiation intensity of green LED light and the effect (part 3). Fig.11A This is a graph showing other test results regarding the relationship between the radiation intensity of green LED light and the effect.

[0046] Fig.12 This is a diagram showing the difference in the effect of suppressing melanin production due to the difference in wavelength (Part 1) of the test results.

[0047] Fig.13 This is a graph showing the difference in the effect of suppressing melanin production due to the difference in wavelength (part 2).

[0048] Fig.14 This is a diagram showing the difference in the effect of suppressing melanin production due to the difference in wavelength (part 3).

[0049] Fig.15 This is a graph showing the test results regarding the expression level of keratin 10.

[0050] Explanation of symbols

[0051] 1: light irradiation device (skin treatment device); 2: gripping part; 3: head; 3a: skin facing part; 30: light irradiation part; 36: LED (Light Emitting Diode); 100: control device (control part). DETAILED DESCRIPTION

[0052] Hereinafter, each embodiment will be described in detail with reference to the drawings.

[0053] Figure 1 It is a perspective view showing the light irradiation device 1 of this embodiment.

[0054] The light irradiation device 1 generates light that can be irradiated to human skin. The light can have a beauty-related effect. In this case, the beauty-related effect is arbitrary and may include hair removal, skin beautification, elimination of sagging, or tightening, fat burning, lifting, face slimming, enhancement of skin elasticity or luster, moisturization, or any combination of more than one of these. In addition, the beauty-related effect may be an effect that can be quantified or an effect that cannot be quantified.

[0055] The functions of the light irradiation device 1 can be realized by a computer in a single light irradiation device 1, or by a combination of a computer in the light irradiation device 1 and an external server and / or a user terminal. In addition, in this case, the computer reading program that realizes the functions of the light irradiation device 1 can be executed by the computer in the light irradiation device 1, or by a combination of a computer in the light irradiation device 1 and an external server and / or a user terminal.

[0056] also, Figure 1 The light irradiation device 1 shown is a portable device that can be held by a user's hand, but may also be applied to a movable type that is movably supported by a fixed device via an arm or the like.

[0057] The light irradiation device 1 includes a grip portion 2 and a head portion 3. In this case, the user grips the grip portion 2 and directs a skin-facing portion 3a (described below) of the head portion 3 toward a desired part of the user's face or body, thereby locally irradiating the desired part with light from the light irradiation device 1.

[0058] The grip portion 2 has a shape that is easy for the user to hold. The grip portion 2 may include an input portion (not shown), which includes various buttons such as a power on / off button or a mode switching button, an intensity adjustment button, etc. In addition, the various buttons may be mechanical buttons or touch switches. In addition, a display portion (not shown) that displays the state of the light irradiation device 1, etc. may also be provided in the grip portion 2.

[0059] The head 3 is disposed at the end of the grip 2. In addition, the head 3 may be fixed relative to the grip 2, may be detachable, or may be movable relative to the grip 2. In addition, a plurality of detachable accessories may be included.

[0060] The head 3 may have a skin-facing portion 3a that is roughly flat or curved (a curved surface with a relatively large radius of curvature). The shape of the skin-facing portion 3a when viewed from the front (the shape when observed in a direction perpendicular to the skin-facing portion 3a) is arbitrary, such as rectangular or circular, elliptical, polygonal, etc. The skin-facing portion 3a may be formed by any material that allows light to pass through, such as glass. In the present embodiment, the skin-facing portion 3a is formed by a light emitting surface. In addition, the skin-facing portion 3a is in a shape in which the light emitting surface is exposed, but may also be covered by a lens, etc. The skin-facing portion 3a is preferably in a concave shape that is concave relative to the skin in the up and down directions. In this case, the concave portion contains cosmetics, etc., which are objects to be penetrated into the skin, and skin treatment can be performed by light irradiation, thereby improving the skin treatment effect.

[0061] A light irradiation unit 30 ( Figure 1 Not shown in the figure, refer to Figure 3 ). The light irradiation section 30 generates light to be irradiated to the skin via the skin facing section 3a. That is, the light irradiation section 30 irradiates light to the skin via the skin facing section 3a. In addition, an optical system such as a light guide may be arranged between the light irradiation section 30 and the skin facing section 3a, or an optical system may not be arranged.

[0062] In this embodiment, the light irradiation unit 30 includes an LED (Light Emitting Diode) 36. Only one LED 36 may be provided, or a plurality of LEDs 36 may be provided. When a plurality of LEDs 36 are provided, the plurality of LEDs 36 may be provided, for example, in a plane substantially parallel to the skin-facing portion 3a.

[0063] In addition, in the present embodiment, the light irradiation device 1 is portable, but it can also be fixed, and the form is arbitrary. In addition, the light irradiation device 1 can be, for example, in the form of a mask attached to the face or in a form that can be wrapped around the body for use. In addition, the irradiation range of the light irradiation device 1 is also arbitrary, and the skin can be irradiated in various ways, such as a wide range irradiation method and a point irradiation method. In addition, the light irradiation device 1 has a head 3 separated from the light irradiation unit 30, but it can also be a structure in which the head 3 and the light irradiation unit 30 are formed as one.

[0064] Figure 2 This is an explanatory diagram of the characteristics of light (predetermined light) generated from the LED 36 , with the wavelength being the horizontal axis and the intensity being the vertical axis, and shows an example of the characteristics.

[0065] LED 36 is preferably a light source that generates a predetermined light having a central wavelength within a wavelength range longer than 490 nm and less than 525 nm. The basis (technical significance) of the superiority of the wavelength range (and the wavelength of approximately 505 nm therein) will be referred to Figure 4A , Figure 4B ,and Fig.12 In addition, 505 nm and its vicinity are strictly blue-green wavelengths, and 525 nm and its vicinity are strictly green wavelengths, but the wavelength range longer than 490 nm and less than 525 nm may be described as "green" below.

[0066] Prescribed light for better imaging Figure 2 As shown, the central wavelength is approximately 505 nm. If the central wavelength is such, the generation of melanin in the irradiated user's skin portion can be suppressed as described below, compared with a case where the central wavelength is not such. For example, the prescribed light can be irradiated in such a manner that the irradiated user's skin portion has a good effect of suppressing the generation of melanin by 5% or more, more preferably 10% or more, compared with a case where the prescribed light is not irradiated.

[0067] The prescribed light is preferably half-width at half maximum (refer to Figure 2 ) is less than ±20nm, more preferably about ±10nm. Thus, it is possible to maximize the effect of suppressing the generation of melanin in the irradiated user's skin area.

[0068] The light irradiation unit 30 preferably emits 0.5 mW / cm 2 Above and 62mW / cm 2 The prescribed light is irradiated at a radiation intensity within the range below, preferably 11.5 mW / cm 2 Above and 30mW / cm 2 The following range of radiation intensity is used to irradiate the specified light. The test results related to this will refer to Figure 8 The following figures are described below.

[0069] In addition, the light irradiation unit 30 preferably provides 0.09 J / cm 2 Above and 30J / cm 2 The prescribed light is irradiated at an irradiation energy in the following range, preferably 0.09 J / cm 2 Above and 11J / cm 2 The prescribed light is irradiated at the irradiation energy in the following range.

[0070] In addition, multiple LEDs 36 may be mounted on one chip. Alternatively, LED 36 may be combined with other LEDs having other central wavelengths to form one chip. For example, when LED 36 and a red LED are formed into one chip LED, the ratio of the number of green LEDs to red LEDs in one chip may be appropriately adjusted.

[0071] Figure 3 It is a diagram showing an outline of a control system of the light irradiation device 1 .

[0072] The light irradiation device 1 includes a control device 100, and a power supply 90 and an LED 36 are electrically connected to the control device 100. The control device 100 operates based on the power from the power supply 90 and controls the LED 36. Under the control of the control device 100, the LED 36 operates based on the power from the power supply 90. The power supply 90 may include an external power supply and / or an internal power supply. In addition, the internal power supply may be a rechargeable battery.

[0073] The control device 100 irradiates the skin with the prescribed light via the LED 36. At this time, the control device 100 can achieve continuous light irradiation for more than 1 minute, with the proportion of the irradiation time of the prescribed light being more than 1 / 2. In this case, light of other wavelengths may be irradiated during the time other than the irradiation time of the prescribed light, or light may not be irradiated (that is, the prescribed light may be irradiated intermittently).

[0074] In addition, the control device 100 can control the irradiation of light from the head 3 in one or more action modes. The one or more action modes may include a prescribed action mode that has established a correspondence relationship with the effect of suppressing the production of melanin, or a prescribed action mode that has established a correspondence relationship with an effect related to the effect of suppressing the production of melanin. In this case, the control device 100 outputs the prescribed light from the head 3 in the prescribed action mode.

[0075] Figure 3A It is a diagram showing an example of the hardware configuration of the control device 100 . Figure 3A In FIG. 1 , the control object 60 is schematically illustrated in association with the hardware configuration of the control device 100 .

[0076] The control device 100 includes: a CPU (Central Processing Unit) 11, a RAM (Random Access Memory) 12, a ROM (Read Only Memory) 13, an auxiliary storage device 14, a drive device 15, and a communication interface 17 connected by a bus 19, and a wired transceiver 25 and a wireless transceiver 26 connected to the communication interface 17.

[0077] The auxiliary storage device 14 is, for example, a HDD (Hard Disk Drive) or an SSD (Solid State Drive), and is a storage device that stores data related to application software and the like.

[0078] The wired transceiver 25 includes a transceiver capable of communicating using a wired network. The control object 60 is connected to the wired transceiver 25. Part or all of the control object 60 may be connected to the bus 19 or to the wireless transceiver 26.

[0079] The wireless transceiver 26 is a transceiver that can communicate using a wireless network. The wireless network may include: a wireless communication network of a mobile phone, the Internet, a VPN (Virtual Private Network), a WAN (Wide Area Network), etc. In addition, the wireless transceiver 26 may include: a near field communication (NFC) unit, a Bluetooth (registered trademark) communication unit, a Wi-Fi (Wireless-Fidelity) transceiver, an infrared transceiver, etc. In addition, the control device 100 can communicate with a server (not shown) via the wireless transceiver 26 to obtain various information.

[0080] In addition, the control device 100 may also be connected to a recording medium 16. The recording medium 16 stores a predetermined program. The program stored in the recording medium 16 is installed in the auxiliary storage device 14 of the control device 100 via the drive device 15. The installed predetermined program can be executed by the CPU 11 of the control device 100. For example, the recording medium 16 may be a recording medium that records information optically, electrically or magnetically, such as a CD (Compact Disc)-ROM, a floppy disk, a magneto-optical disk, etc., a semiconductor memory that records information electrically, such as a ROM, a flash memory, etc. In addition, the recording medium 16 does not include a carrier wave.

[0081] Next, the effect of the predetermined light (the effect of suppressing the production of melanin) will be further described with reference to test results and the like.

[0082] Since visible light is less invasive to organisms, various studies have been conducted on the effects of visible light on organisms in order to apply visible light to the medical field or the field of skin beauty (Imagawa et al., The Journal of Japan Society for Laser Surgery and Medicine, 32, 444 (2012)). For example, there are also reports that red light plays an important role as a means of preventing skin aging, and green light and yellow light play an important role as a means of inhibiting excessive cell activity.

[0083] Melanin, which causes pigmentation in the skin, is produced in melanocytes and plays an important role in protecting DNA (deoxyribonucleic acid) from damage by harmful ultraviolet rays. However, it also causes chloasma, so there is a high demand for improving melanin. Therefore, we conducted an experiment to verify whether green LED light, which inhibits cell activity, can also affect the activity of melanoma and reduce the amount of melanin produced.

[0084] In order to verify the effect of inhibiting the production of melanin obtained by using green LED, the inventor of this case commissioned Toin Yokohama University to conduct the following test. The verification of the effect of inhibiting the production of melanin was carried out using B164A5 cells from mice (B16 melanoma cells, RIKEN BRC) and melanoma cells from humans (HMV-II cells, KAC Co., Ltd.) obtained from Toin Yokohama University. In addition, the light source of green LED used SMT525 (wavelength 525nm) and SMT505 (wavelength 505nm) manufactured by Ushio Electric.

[0085] (Based on cell viability of B16 melanoma cells)

[0086] B16 melanoma cells were expressed at 1×10 4 cells / mL and 2×10 4 Cells / mL were seeded into 6-well plates and incubated at 37°C, 5% CO 2 After culturing for 3 days, replace with a culture medium without phenol red. Irradiate with green LED once a day for 3 days and then culture for 1 day. Then, add a cell counting kit (Cell Counting Kit-8 (manufactured by Tongren Chemical Co., Ltd.)) and culture for 3 hours. After culturing, dispense the culture medium, measure the absorbance at 450nm, and calculate the number of viable cells. The greater the absorbance at 450nm, the greater the number of viable cells.

[0087] (Evaluation based on inhibition of melanin production in B16 melanoma cells)

[0088] B16 melanoma cells were expressed at 1×10 4 cells / mL and 2×10 4 Cells / mL were seeded into 6-well plates and incubated at 37°C, 5% CO 2After culturing for 3 days, replace it with a phenol red-free medium containing 100nM of melanin synthesis inducer α-MSH. Irradiate with green LED once a day for 3 days, culture for 1 day, then wash the cells with 1mL of phosphate buffered saline (PBS(-)), dissolve the cells with a 2mol / L sodium hydroxide aqueous solution containing 10wt% dimethyl sulfoxide (DMSO), and measure the amount of melanin produced based on the absorbance at 405nm. Furthermore, use a protein analyzer (RC DCTM protein analyzer (manufactured by BioRad)) to measure the amount of protein from the cells. Based on the measurement results, calculate the amount of melanin per unit amount of protein from the cells.

[0089] (The effect of inhibiting the production of melanin obtained by irradiation with green LED light)

[0090] It was confirmed that when irradiated with green LED light of wavelength 505nm and 525nm, the survival number of B16 melanoma cells decreased and the amount of melanin produced decreased. The above tendency was more significant with green LED light of 505nm. Figure 4A and Figure 4B The test results shown are clear. Figure 4A This is a graph showing the number of surviving B16 melanoma cells when irradiated with green LED light having a wavelength of 505 nm. Figure 4B This is a graph showing the number of B16 melanoma cells that survived when irradiated with green LED light of 525 nm wavelength. 4 (cells / mL), and the initial cell concentration was 2×10 4 (cells / mL), the concentration was measured after 30 minutes and 60 minutes.

[0091] (Evaluation of melanin production inhibition based on human HMV-II melanoma cells) The report states that mouse B16 melanoma cells and human HMV-II melanoma cells have different drug sensitivities to whitening agents. In the evaluation of B16 cells, the effect of inhibiting melanin production was significant with 505nm green LED light, so the wavelength was focused on 505nm for HMV-II cells to evaluate the effect of the light.

[0092] Unlike B16 cells, HMV-II cells have a low proliferation effect on melanoma cells when added with the melanin synthesis inducer α-MSH. Therefore, in the evaluation of HMV-II cells, theophylline, which is an enhancer of MSH, was used as a melanin synthesis inducer.

[0093] 1×10 4The HMV-II cell dispersion with a concentration of 1×10 cells / mL was dispensed into a 66-well plate in 2 mL units. 4 Cells / mL were inoculated and incubated at 37°C and 5% CO 2 After 3 days of culture at 40mM, the cells were cultured and then replaced with 2mL of phenol red-free culture medium containing 25μL of theophylline. The cells were irradiated with 505nm LED light once a day for 3 days, and then cultured for 1 day. A 2M NaOH solution containing 10wt% dimethyl sulfoxide (DMSO) was added to 300μL. The cells were dissolved with a 2mol / L sodium hydroxide aqueous solution, and the melanin production was measured based on the absorbance at 405nm. Furthermore, a protein analyzer (RC DCTM protein analyzer (manufactured by BioRad)) was used to measure the amount of protein from the cells. Based on the measurement results, the amount of melanin per unit of protein from the cells was calculated.

[0094] (Based on the survival rate of HMV-II cells)

[0095] HMV-II cells were cultured at 1×10 4 Cells / mL were seeded into 6-well plates and incubated at 37°C, 5% CO 2 After culturing for 3 days, replace it with a medium that does not contain phenol red. Irradiate with a 505nm LED once a day for 3 days and then culture for 1 day. Then, add a cell counting kit (Cell Counting Kit-8 (manufactured by Tongren Chemical Co., Ltd.)) and culture for 3 hours. After culturing, dispense the culture medium and measure the absorbance at 450nm. After the measurement, calculate the survival rate as a relative value relative to the absorbance at 450nm when no LED light is irradiated.

[0096] (The effect of inhibiting the production of melanin obtained by irradiation with green LED light)

[0097] It was confirmed that when irradiated with 505 nm LED light, the survival rate of HMV-II cells decreased and the amount of melanin produced decreased.

[0098] For both B16 cells and HMV-II cells, it was verified that green LED light irradiation is effective in inhibiting the production of melanin. FIG. 5A to FIG. 5D Other test results are shown. FIG. 5A to FIG. 5D The other tests shown are also the same as those mentioned above. Figure 4A and Figure 4B The test was carried out in the same manner. Figure 5E Is FIG. 5A to FIG. 5DAs can be seen from these figures, 505nm has a higher effect of inhibiting the production of melanin than 525nm, and 20 minutes is more significant than 10 minutes. In addition, when it is 505nm, inhibition is seen at 30 minutes and 60 minutes, but when it is 525nm, no inhibitory effect is seen at 30 minutes, but inhibition is seen at 60 minutes.

[0099] This example confirms that green LED light, especially light with a wavelength of 505 nm, has an effect of suppressing the production of melanin that causes chloasma. 6A to 7B The test results shown are clear. Fig. 6A and Figure 6B Respectively represent the first and second test results, Fig. 7A and Figure 7B The results of the first and second tests are shown respectively. 6A to 7B The other tests shown are also the same as those mentioned above. Figure 4A and Figure 4B The described experiments were carried out identically.

[0100] Figures 8 to 11 is a graph showing other test results. Figure 8 This is a table showing the relationship between the radiation intensity of green LED light and the effect (the effect of suppressing the production of melanin). Figures 9 to 11 This is a graph showing the relationship between the radiation intensity of green LED light and the effect. Fig.11A This is a graph showing other test results related to the relationship between the radiation intensity and effect of green LED light.

[0101] Figures 8 to 11 The other experiments shown were performed as follows.

[0102] B16 melanoma 4A5 uses cells provided by RIKEN BRC. For the contents of this experiment, the term "cells" shown below refers to the B16 melanoma 4A5. Cell culture was performed using the cells described in the following steps S1 to S3, and the following culture medium was used as the culture medium. Dulbecco's Modified Eagle Medium (DMEM, Cat No. 10566-016, Gibco, USA) containing 10.0% (v / v) fetal bovine serum (Fetal Bovine Serum) (FBS, Cat No. SH30071.03, Hyclone (registered trademark), UK) and 1.0% (v / v) antifungal agent (Antibiotic-Antimycotic 100X, Cat No. 15240-062, Invitrogen, USA) was used. A culture medium (DMEM) containing 10% fetal bovine serum (FBS) and 100 nM α-melanocyte stimulating hormone (α-MSH, Cat No. M4135, Sigma-Aldrich, USA) and 100 μM theophylline (Cat No. T1633, Sigma-Aldrich, USA) was prepared.

[0103] Step S1: Cell culture and subculturing at 3.0×10 5 Cells were seeded into 60 mm dishes (Cat No. 353002, Falcon (registered trademark), USA) and incubated in CO 2 Incubator (CO 2 concentration = 5%, 37°C) for 24 hours.

[0104] Step S2: Green LED light irradiation

[0105] The culture medium was removed, and after washing with phosphate buffer saline (PBS (-), Cat No. 198601, Nissui, Japan), it was replaced with 8 mL of balanced salt solution (+) (Hanks balanced salt solution (+)) (HBSS (+), Cat No. 084-08965, Wako, Japan), and then an irradiation machine was applied according to the irradiation conditions. Furthermore, the balanced salt solution (+) (HBSS (+)) was removed and replaced with 3 mL of test culture medium for 72 hours. In addition, regarding the irradiation conditions, the irradiation time was set to 3 minutes.

[0106] Step S3: After the culture, the cells were washed with phosphate buffered saline (PBS), and then treated with 2 mL of a cell viability detection reagent solution (alamarBlue detection reagent, alamarBlue is a registered trademark) (Cat No. DAL1100, Invitrogen (registered trademark), USA) diluted 10 times with a serum-free medium (serum-free DMEM) (hereinafter also referred to as "alamarBlue solution"), and incubated at 4 °C for 1 h. 2 Culture in an incubator at 37°C for 2 hours. Recover the alamarBlue solution and add 200μL to a 96-well plate (Cat No.9017, costar, USA), and use a microplate reader (SPARK (registered trademark) 10M, TECAN, Switzerland) to measure the absorbance at 570nm and 600nm (OD570, OD600). As a blank group, use alamarBlue solution. Remove the alamarBlue solution from the 60mm plate, wash with phosphate buffered saline (PBS (-)), and in order to solubilize the melanin, add 1mL of a 1M sodium hydroxide aqueous solution containing 10% dimethyl sulfoxide (DMSO), and culture at 85°C for 10 minutes. Add 100μL of the melanin solution to a 96-well plate, and use a microplate reader to measure the absorbance at 405nm (OD405). Set the OD570-600 as a control to 100%, and calculate the cell survival rate of the LED application group. In addition, the OD405 used as the control was set to 100% and the melanin production rate was calculated. Furthermore, the value obtained by dividing the OD405 of the control and LED irradiation application groups and the OD570-600 measured using the cell viability detection reagent (alamarBlue assay) was calculated as the melanin production rate of each cell. The difference significance test was performed using an uncorrelated t-test for the control and LED application groups. The test set the significance level to less than 5% on both sides.

[0107] If Figures 8 to 11 It can be seen that when B16 melanoma cells were irradiated with green LED (505nm), the melanin production rate at any output was significantly lower than that of the control, confirming that melanin production was inhibited. 2 ~11.5mW / cm 2 The highest output of the application is 11.5mW / cm 2 When the melanin production rate of each cell was significantly lower than that of the control, it can be seen that the one with the highest output has a stronger tendency to suppress the production ability under this condition. Based on these contents, as described above, the radiation intensity of the prescribed light from the light irradiation unit 30 can be preferably 0.5 mW / cm 2 The range is more preferably 11.5 mW / cm2 above.

[0108] in addition, Fig.11A The difference in the effect of irradiating green LED light with a wavelength of 520nm with different radiation intensities is shown in FIG. Fig.11A The test shown is also based on Figure 8 And so on to implement in the same way. Fig.11A In the experiment, the control group represents the test results without any light exposure. Here, 9mW / cm 2 With 62W / cm 2 The results of each test were compared. Graphs A, B, and C correspond to cell survival rate, melanin production rate, and melanin production rate of each cell type, respectively.

[0109] As Fig.11A It can be seen that when the radiation intensity of the prescribed light from the light irradiation unit 30 is higher than a certain level, the effect will not be significantly increased (that is, saturation). Based on these contents, as described above, the radiation intensity of the prescribed light from the light irradiation unit 30 can be preferably 0.5 mW / cm 2 Above and 62mW / cm 2 The following range, more preferably 11.5 mW / cm 2 Above and 62mW / cm 2 In addition, from the perspective of power consumption, it can also be said that it can replace 62mW / cm 2 , the ideal is 30mW / cm 2 The upper limit value of around.

[0110] In addition, Figures 8 to 11A In the test shown, the irradiation energy of the predetermined light from the light irradiation unit 30 is 0.09 J / cm 2 Above and 11J / cm 2 In addition, considering the other test results disclosed in this specification, the irradiation energy of the predetermined light from the light irradiation unit 30 is 0.09 J / cm 2 Above and 45J / cm 2 The test was conducted within the following range. Furthermore, the irradiation energy was relatively small at 0.09 J / cm 2 Above and 11J / cm 2 Effective effects were also confirmed within the following ranges.

[0111] In addition, the light irradiation device 1 can irradiate the green LED light to the dry skin of the user, but can also irradiate the green LED light to the skin after the gel or liquid containing the penetration object to the skin is applied. In this case, the object as the penetration object is arbitrary. The object is a substance that can be applied to the skin of a person, and typically can be a substance that can expect various effects such as beauty effects.

[0112] Secondly, refer to Fig.12 The following figures further explain the preferred wavelength range of light irradiated onto the skin.

[0113] References in previous text Figure 4A and Figure 4B The above-mentioned test showed that when irradiated with green LED light of wavelengths of 505nm and 525nm, the number of viable B16 melanoma cells decreased and the amount of melanin produced decreased.

[0114] Fig.12 It is a diagram showing other test results, showing the test results when LED light having wavelengths of 450 nm, 520 nm, and 850 nm (near infrared wavelength) is irradiated. Fig.12 In the experiment, the cell survival rate, melanin production rate, and melanin production rate of each cell type are shown, respectively, when the wavelength of LED 36 is 450nm, 520nm, and 850nm. Fig.12 The control group represents the test results without any light exposure.

[0115] also, Fig.12 The test results shown are based on the same reference as above. Figure 4A and Figure 4B The tests are carried out using different devices, but the test methods are essentially the same.

[0116] If Fig.12 It can be seen that excellent melanin production inhibition was confirmed at wavelengths of 520nm and 450nm. In other words, it was shown that the number of survival of B16 melanoma cells decreased and the amount of melanin produced decreased. In addition, it can be seen that the effect of inhibiting melanin production at 520nm may be higher than that at a wavelength of 450nm. In addition, a slight inhibition tendency was seen at a wavelength of 850nm, but there was no significant difference, and the result was the lowest.

[0117] Fig.13 This is a graph showing still another test result, showing the differences in the effect of suppressing melanin production caused by the differences in three wavelengths. Fig.13 In the figure, the melanin production rate of each cell type is shown, and the test results of the case where the wavelengths of LED 36 are 505nm, 525nm, and 630nm (red wavelength) are respectively irradiated. In addition, Fig.13 The control group also represents the test results without any light exposure.

[0118] also, Fig.13 The test results shown are based on the same reference as above. Figure 4A and Figure 4B The tests are carried out using different devices, but the test methods are essentially the same.

[0119] As Fig.13 It is found that at a wavelength of 525 nm or 630 nm, no significant suppression of melanin production is observed as at a wavelength of 505 nm.

[0120] Fig.14 This is a graph showing still another test result, showing the differences in the effect of suppressing melanin production caused by the differences in four wavelengths. Fig.14 In the figure, the cell survival rate is shown as the test results of the case where the wavelength of LED 36 is 470nm (blue wavelength), 490nm, 505nm, and 525nm. In addition, Fig.14 The control group also represents the test results without any light exposure.

[0121] also, Fig.14 The test results shown are based on the same reference as above. Figure 4A and Figure 4B The tests are carried out using different devices, but the test methods are essentially the same.

[0122] As Fig.14 It can be seen that the survival rate of B16 melanoma cells is lower at wavelengths of 470nm, 505nm and 525nm than at wavelength 490nm. In other words, the superiority of wavelengths of 470nm, 505nm and 525nm over wavelength 490nm (superiority in the effect of suppressing melanin production) is shown.

[0123] Fig.15 This is a graph showing the test results regarding the expression level of keratin 10. Fig.15 , regarding the expression level of keratin 10, test results are shown for the cases where the wavelengths of the LED 36 are irradiated at 470 nm, 505 nm, and 590 nm (yellow wavelength), respectively.

[0124] An outline of the test method is as follows.

[0125] (Step S1) Cell pre-culture

[0126] Human epidermal keratinocytes (NHEK) were revived in T-75 flasks using culture medium and cultured under CO 2 Incubator (5% CO 2, 37°C, humidified). When the cells reach about 80% fusion, they are subcultured in a T-225 flask and cultured until the necessary number of cells is obtained before use in subsequent experiments. The cell subculture method is as follows. After washing the cells with phosphate buffered saline (PBS (- / -)), 0.05% trypsin-ethylenediaminetetraacetic acid (trypsin-EDTA (Ethylene Diamine Tetraacetic Acid)) is used to peel off the cells, and trypsin neutralization solution is added to neutralize the trypsin. Secondly, the cell suspension is recovered and placed in a centrifuge tube for centrifugation (room temperature, 180xg, 5min). The supernatant is removed, the culture medium is added again to suspend the cells, and the number of cells is counted. Use the culture medium to suspend the cells to the target cell density and inoculate them into the culture vessel used for the experiment.

[0127] (Step S2) Cell Treatment

[0128] Cells were seeded into 60 mm dishes at 900,000 cells / dish / 3 mL. On the second day of seeding, 5 mL of culture medium was added to a total of 8 mL, and the machine treatment was performed for 30 seconds. The treatment was performed every 24 hours ± 1 hour, and a total of 3 beauty device treatments were performed. Calcium chloride was added in the same manner as in the initial machine treatment, with the culture medium set to 8 mL. Then, the treatment was performed for 72 hours.

[0129] (Step S3) RNA (ribonucleic acid) extraction: Using cells 24 hours after the final machine treatment, RNA extraction was performed using a total RNA mini-extraction kit (RNeasy Plus Mini Kit). The method was based on the operating instructions of the kit. After RNA extraction, the concentration was measured using a spectrophotometer (NanoDrop Eight) and stored at -80°C.

[0130] (Step S4) Quantitative polymerase chain reaction (quantitative PCR)

[0131] One-step real-time RT-PCR was performed using the probe reverse transcription polymerase chain reaction kit (QuantiTect Probe RT-PCR Kit) attached to the probe kit (FastLane Cell Probe Kit) according to the composition in the table below. The RNA sample was diluted to about 100 ng / μL. The RT-PCR reaction was performed under the conditions of 50°C·30min-95°C·15min-(94°C·15sec-60°C·60sec)×40 cycles. As an internal standard gene, the glyceraldehyde-3-phosphate dehydrogenase gene (i.e., GAPDH gene) was used.

[0132] If Fig.15 It can be seen that the expression amount of keratin 10 is significantly increased at a wavelength of 505 nm compared with wavelengths of 470 nm and 590 nm. In other words, the superiority of the wavelength of 505 nm over the wavelengths of 470 nm or 590 nm (superiority of the expression effect of keratin 10) is shown.

[0133] Here, the mechanism of metabolic turnover is explained. The epidermis is arranged in four layers: basal layer, spinous layer, granular layer, and stratum corneum. Metabolic turnover at the cellular level is carried out through the following four stages: cell proliferation in the basal layer, keratin (K10) synthesis in the spinous layer, cell death (apoptosis and necrosis) in the granular layer, and cleavage by protease (KLK8) in the stratum corneum. The following process is called metabolic turnover, that is, in the human skin, epidermal cells are generated in the basal layer, mature while moving to the upper layer, and peeled off in the stratum corneum.

[0134] If the synthesis of keratin decreases, the structure of the epidermis cannot be maintained and wrinkles or sagging will occur. In a rapid metabolic turnover, immature epidermal cells are exposed to the outside world before they become keratin. In addition, excessive metabolic turnover can cause the pathological condition of atopic dermatitis. In a slow metabolic turnover, keratin that should be peeled off due to dirt remains and becomes thick. Furthermore, abnormal delays can cause psoriasis or keratosis, etc. Therefore, indicators other than cell proliferation are also indispensable factors in considering normal metabolic turnover.

[0135] Thus, according to the mechanism of metabolic turnover, keratin 10 is expressed in the spinous layer and is a useful substance in the process of metabolic turnover. The expression of keratin 10 indicates that normal metabolic turnover is taking place.

[0136] In addition, there is also a paper reporting that blue light irradiation is used to inhibit the conduction pathway of TGF-β signals, thereby inhibiting cell proliferation and collagen expression in human skin fibroblasts (Ge Ge et al., "Induced skin aging by blue-light irradiation in human skin fibroblasts via TGF-β, JNK and EGFRpathways", Journal of Dermatological Science). From this aspect, it can also be strongly speculated that a wavelength of 505 nm is more advantageous than a wavelength of 470 nm.

[0137] By the above Figure 4A , Figure 4B and Figures 12 to 15 From the test results shown, it can be seen that irradiating the skin with light having a central wavelength in a wavelength range longer than 490nm and less than 525nm is more advantageous from the perspective of inhibiting the production of melanin or the expression of keratin 10 than irradiating the skin with light having a central wavelength in other wavelength ranges.

[0138] Although each embodiment has been described in detail above, the present invention is not limited to a specific embodiment, and various modifications and changes can be made within the scope of the claims. In addition, all or a plurality of the constituent elements of the above-described embodiments can be combined.

Claims

1. A skin treatment device, characterized in that: comprising a light source that generates predetermined light having a central wavelength within a wavelength range longer than 490 nm and not more than 525 nm, and capable of irradiating the skin with the predetermined light from the light source, The half-width at half maximum of the prescribed light is less than ±20nm, The specified light is the light emitted by the LED light source.

2. The skin treatment device according to claim 1, characterized in that The prescribed light has a central wavelength of approximately 505 nm.

3. The skin treatment device according to claim 1 or 2, characterized in that: At 0.5mW / cm 2 Above and 62mW / cm 2 The prescribed light is irradiated at the following radiation intensity.

4. The skin treatment device according to claim 3, characterized in that: The radiation intensity is 11.5 mW / cm 2 Above and 62mW / cm 2 the following.

5. The skin treatment device according to claim 1 or 2, characterized in that: At 0.09J / cm 2 Above and 45J / cm 2 The prescribed light is irradiated with an irradiation energy in the following range.

6. The skin treatment device according to claim 5, characterized in that: The irradiation energy is 0.09 J / cm 2 Above and 11J / cm 2 the following.

7. The skin treatment device according to claim 1 or 2, characterized in that: The light source includes an LED element.

8. The skin treatment device according to claim 7, characterized in that: A plurality of the LED elements are mounted on one chip.

9. The skin treatment device according to claim 1, characterized in that: The light source is provided so that the predetermined light can be irradiated in such a manner as to suppress the generation of melanin in a skin area of ​​a person to be irradiated, compared with light having a central wavelength outside the wavelength range.

10. The skin treatment device according to claim 1 or 2, characterized in that: The light source is provided so that the predetermined light can be irradiated in such a manner that the skin part of the person to be irradiated has an effect of suppressing the generation of melanin by 5% or more compared with a case where the predetermined light is not irradiated.

11. The skin treatment device according to claim 1 or 2, characterized in that: It further includes a control unit that controls the irradiation of light in one or more action modes, wherein the one or more action modes have a prescribed action mode that establishes a correspondence with the effect of inhibiting the production of melanin, and the control unit is configured to output the prescribed light in the prescribed action mode.

12. The skin treatment device according to claim 1 or 2, characterized in that: The light source is provided so as to realize continuous light irradiation of more than one minute, with the ratio of the irradiation time of the predetermined light being more than 1 / 2.

13. The skin treatment device according to claim 1, characterized in that: The light source is configured such that the prescribed light from the light source can promote the expression of keratin 10.

14. The skin treatment device according to claim 1, characterized in that: The light source is arranged to be controlled so that, in an operation mode of the skin treatment device for irradiating a wavelength range longer than 490 nm and not more than 525 nm, the total irradiation time in the operation mode is at least 3 minutes.

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