Kit and blood analyzer for POCT

Abstract

The utility model discloses a kit and a blood analyzer for POCT, and belongs to the POCT blood detection technical field, the kit comprises a box body and a sealing cover, the sealing cover is arranged on the box body, a first cavity, a second cavity, a third cavity and a micropore structure are arranged in the box body, the micropore structure is arranged between the first cavity and the second cavity, and the first cavity is communicated with the second cavity. The first cavity is communicated with the first cavity, the second cavity is communicated with the third cavity, a liquid sample enters the second cavity from the first cavity to start metering, and when liquid enters the third cavity from the second cavity, metering is stopped, so that the accuracy and consistency of liquid sample metering are improved, and automatic metering is realized without manual operation.

Description

Technical Field

[0001] The utility model relates to the technical field of POCT blood detection, in particular to a reagent kit and a blood analyzer used for POCT. Background Art

[0002] Routine blood analyzers can perform clinical analysis and measurement of red blood cells, white blood cells, platelets and hemoglobin in the blood, so blood cell analyzers are widely used in clinical practice. Existing blood cell analyzers are based on the Coulter principle for cell counting. The analyzer includes a main control device, a liquid circuit system, a sensor detection device, a blood parameter analysis device, a display device and a cleaning device. The blood analysis process is sampling, dilution, measurement, calculation, display and cleaning in sequence. After the blood is diluted and mixed with the corresponding reagents, it is measured through the microporous channel in the detection sensor device. The measurement signal is uploaded to the analyzer for calculation, and finally the reading result is obtained; after each test, the entire liquid circuit system needs to be fully cleaned, and the components after cleaning need to be checked to see if they are clean to meet the needs of the next blood test. However, cleaning is not the core device part of the blood analyzer to accurately detect blood cells, and the cleaning procedure is time-consuming and uses a large amount of reagents, resulting in a lot of waste; in addition, the cleaning system has a complex structure, a large volume, and requires several external flushing liquid and cleaning liquid reagent barrels, which is cumbersome to operate and has a high failure rate of the instrument. A disposable blood analyzer that does not require cleaning and is easy to operate is now needed.

[0003] The existing patent application with publication number CN115248323A discloses a detection seat and a POCT blood cell analyzer, wherein the detection seat includes a shielding box, and a first optical component and a second optical component arranged on the shielding box, wherein the first optical detection component includes a first transmitting end and a first receiving end arranged at intervals, and a first optical detection position is formed between the first transmitting end and the first receiving end; the second optical detection component includes a second transmitting end and a second receiving end arranged at intervals, and a second optical detection position is formed between the second transmitting end and the second receiving end; the first optical detection position and the second optical detection position are staggered. The staggered first optical detection position and the second optical detection position can realize simultaneous detection of multiple samples to be detected, but the blood analyzer has poor sealing performance, and the detection process cannot be automatically started and stopped, and the operation is cumbersome. Utility Model Content

[0004] One of the purposes of the utility model is to provide a reagent box, which solves the problems of poor sealing performance and inability to automatically start and stop metering of the existing reagent box.

[0005] In order to achieve the above utility model purpose, the technical solution adopted by the utility model is as follows:

[0006] A test kit comprises a box body and a cover, wherein the cover is arranged on the box body, wherein a first cavity, a second cavity, a third cavity and a microporous structure are arranged in the box body, wherein the microporous structure is arranged between the first cavity and the second cavity, and the second cavity is connected to the third cavity, and measurement is started when a liquid sample enters the second cavity from the first cavity, and measurement is terminated when the liquid enters the third cavity from the second cavity, thereby improving the accuracy and consistency of liquid sample measurement, and automatically performing measurement without manual operation.

[0007] Furthermore, a fourth cavity is provided in the box body, the fourth cavity is connected to the third cavity, and the fourth cavity is used to absorb waste liquid after detection to prevent liquid leakage and contamination.

[0008] Furthermore, it also includes a first electrode, a second electrode and a third electrode. The first electrode penetrates the bottom wall of the first cavity and is arranged in the first cavity. The second electrode penetrates the bottom wall of the second cavity and is arranged in the second cavity. The third electrode penetrates the bottom wall of the third cavity and is arranged in the third cavity. It is used to transmit electrical signals for starting and ending detection, and the response is rapid, thereby improving the accuracy of detection.

[0009] Preferably, it further comprises a negative pressure port, wherein the negative pressure port is arranged in the fourth cavity, and the liquid sample is moved by the suction force generated by the negative pressure as a driving force.

[0010] More preferably, it also includes an air guide column, which is arranged on the bottom wall of the fourth cavity, part of the air guide column is in the fourth cavity and close to the cover, and the other part protrudes from the bottom surface of the box body to prevent the liquid sample from overflowing outward through the negative pressure port, thereby facilitating docking with an external air source.

[0011] Preferably, the microporous structure is a microporous sheet, a through groove is provided between the first cavity and the second cavity, the microporous sheet is embedded in the through groove, and the quantifiable object can be changed by replacing the microporous sheet, thereby expanding the use scope of the test kit.

[0012] Preferably, a stopper is further included, and a first through hole is provided on the cover, the first through hole is opposite to the first cavity, and the stopper is clamped on the first through hole for injecting liquid samples to ensure the sealing of the box body.

[0013] Preferably, a side cover is further included, a second through hole is provided on the side wall of the box body, the second through hole is communicated with the first cavity, and the side cover is arranged on the second through hole to facilitate the removal of waste liquid.

[0014] Furthermore, a photoelectric detection position is also provided on the box body, and the photoelectric detection position is arranged close to the first cavity and is used for detecting the component ratio of the liquid sample by photoelectric reaction.

[0015] The second purpose of the utility model is to provide a POCT blood analyzer, which solves the problem that the existing blood analyzer cannot support automatic start and stop and the detection liquid is prone to leakage and pollution.

[0016] A blood analyzer for POCT comprises the reagent kit, and the operation steps and consumption are reduced by replacing the reagent kit.

[0017] The beneficial effects of the utility model are:

[0018] (1) The box body of the test kit is provided with a first cavity, a second cavity and a third cavity which are connected to each other respectively. A microporous structure is provided between the first cavity and the second cavity for Coulter counting. The second cavity and the third cavity are connected. When the detection liquid enters the second cavity from the first cavity, the reading starts. When the detection liquid enters the third cavity from the second cavity, the counting stops. By detecting the flow position of the liquid, the start and stop of the detection can be accurately controlled, thereby improving the consistency and accuracy of blood detection.

[0019] (2) The first cavity, the second cavity and the third cavity on the test kit are all provided with a first electrode, a second electrode and a third electrode for transmitting electrical signals, which can directly transmit the start and stop signals of the test to the blood test. In addition, a fourth cavity is also provided in the box body. The fourth cavity is provided with a negative pressure hole to provide power for the flow of liquid samples. It can also be used as a waste liquid cavity to store the tested liquid samples to prevent the samples from leaking out and causing contamination. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 An axonometric diagram of the test kit provided by the utility model;

[0021] Figure 2 A front view of the test kit provided by the utility model;

[0022] Figure 3 A side view of the test kit provided by the utility model;

[0023] Figure 4 A bottom view of the test kit provided by the utility model;

[0024] Figure 5 for Figure 2 Section view along line AA;

[0025] Figure 6 for Figure 3 Sectional view along line BB;

[0026] Figure 7 for Figure 3 Cross-section along line CC.

[0027] Reference numerals:

[0028] 1. Box body; 11. Second through hole; 111. First electrode; 112. Second electrode; 113. Third electrode; 114. Positioning block; 115. Positioning groove; 116. Photoelectric detection position; 12. First cavity; 13. Second cavity; 14. Third cavity; 15. Through groove; 16. Fourth cavity; 17. Liquid channel; 18. Negative pressure port; 181. Air guide column; 2. Cover; 21. First through hole; 3. Microporous structure; 4. Side cover; 5. Plug. DETAILED DESCRIPTION

[0029] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the application, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0030] Embodiment 1

[0031] like Figure 1-Figure 7 As shown, this embodiment discloses a reagent kit, including a box body 1 and a cover 2, the cover 2 is arranged on the box body 1, and a first cavity 12, a second cavity 13, a third cavity 14 and a microporous structure 3 are arranged in the box body 1, the microporous structure 3 is arranged between the first cavity 12 and the second cavity 13, the second cavity 13 is connected to the third cavity 14, a certain voltage is applied between the holes on the microporous structure 3, when the particles pass through the pores, the current changes due to the change in resistance, and the current change signal is converted into particle size data, so as to measure the particle size, when the liquid sample is injected from the first cavity 12 into the second cavity 13, the particles in the liquid sample are measured, and when the liquid sample enters the third cavity 14 from the second cavity 13, the measurement of the liquid sample is terminated.

[0032] Furthermore, a fourth cavity 16 is provided in the box body 1, and the fourth cavity 16 is connected to the third cavity 14. The fourth cavity 16 and the third cavity 14 are connected by a liquid channel 17. The liquid channel 17 is arranged close to the cover 2, so that the liquid sample enters the fourth cavity 16 after filling the third cavity 14, which plays the role of collecting waste liquid and preventing the liquid sample from leaking out.

[0033] Preferably, the volume of the fourth cavity 16 is twice that of the first cavity 12 to prevent excess liquid sample from overflowing.

[0034] Furthermore, it also includes a first electrode 111, a second electrode 112 and a third electrode 113. The first electrode 111 penetrates the bottom wall of the first cavity 12 and is arranged in the first cavity 12. The second electrode 112 penetrates the bottom wall of the second cavity 13 and is arranged in the second cavity 13. The third electrode 113 penetrates the bottom wall of the third cavity 14 and is arranged in the third cavity 14. When the circuit between the first electrode 111 and the second electrode 112 is connected, the blood analyzer connects the metering circuit. When the circuit between the first electrode 111 and the third electrode 113 is connected, the blood analyzer disconnects the metering circuit, thereby obtaining metering data with high accuracy and consistency.

[0035] Preferably, a positioning block 114 is provided at the bottom of the box body 1, the positioning block 114 protrudes from the box body 1, the positioning block 114 semi-surrounds the third electrode 113, the positioning block 114 is used for positioning the bottom of the reagent box, and a positioning groove 115 is provided on the side wall of the box body 1, and the positioning groove 115 is used for guiding and positioning when the reagent box is installed.

[0036] Furthermore, it also includes a negative pressure port 18, which is arranged in the fourth cavity 16. The negative pressure port 18 sucks out the gas in the fourth cavity 16, the third cavity 14 and the second cavity 13, so that the liquid sample in the first cavity 12 flows under the action of negative pressure, providing power for the flow of the liquid sample. By controlling the amount of sucked out air, the circulation rate of the liquid sample can be controlled to ensure the accuracy and consistency of the detection.

[0037] Among them, the negative pressure formed at the negative pressure port 18 is between -10kpa and -40kpa. At the beginning of the test, a lower negative pressure is sampled, such as -5kpa to -15kpa, so that the liquid slowly enters the test cavity to avoid bubbles generated by the agitation of the liquid sample during the test. After a certain period of time, the negative pressure increases, such as -25kpa to -40kpa, which can speed up the test.

[0038] Preferably, it also includes an air guide column 181, which is arranged on the bottom wall of the fourth cavity 16. Part of the air guide column 181 is in the fourth cavity 16 and close to the cover 2 to prevent the liquid sample from being sucked out, and the other part protrudes from the bottom surface of the box body 1 to facilitate positioning and connection with an external negative pressure supply device.

[0039] Preferably, the microporous structure 3 is a microporous sheet, a through groove 15 is provided between the first cavity 12 and the second cavity 13, the microporous sheet is embedded in the through groove 15, and the microporous sheet is provided with micropores for liquid samples to flow through, and the diameter of the micropores is between 50-100 μm, which is selected according to the sample to be tested.

[0040] Preferably, a stopper 5 is further included. The cover 2 is provided with a first through hole 21 . The first through hole 21 is opposite to the first cavity 12 for adding liquid. The stopper 5 is snap-fitted to the first through hole 21 .

[0041] Preferably, a side cover 4 is further included. A second through hole 11 is provided on the side wall of the box body 1. The second through hole 11 is communicated with the first cavity 12. The side cover 4 is arranged on the second through hole 11 for sealing.

[0042] Furthermore, a photoelectric detection position 116 is further provided on the box body 1 , and the photoelectric detection position 116 is arranged close to the first cavity 12 . Specifically, two side walls of the first cavity 12 are arranged to be transparent for detecting light penetrating the first cavity 12 .

[0043] Preferably, the stopper 5 is made of a flexible material, which is convenient for the pipette to penetrate and add the liquid sample into the first cavity 12 , and can automatically shrink and close after the liquid sample is added.

[0044] Embodiment 2

[0045] This embodiment also discloses a blood analyzer for POCT, including a test kit and an analyzer body. The test kit can be detachably connected to the analyzer body, which is convenient for quick plugging and unplugging, simplifies the operation steps of blood testing and prevents leakage of blood samples.

[0046] The working process of the blood analyzer is as follows:

[0047] A certain amount of processed liquid sample is taken and added into the first cavity 12 through the first through hole 21. The reagent kit after adding the sample is installed on the blood analyzer, and the positioning block 114 is aligned with the corresponding position on the blood analyzer. The positioning groove 115 guides to ensure that the first electrode 111, the second electrode 112 and the third electrode 113 are connected with the corresponding electrodes. The blood analyzer is started, and the blood analyzer emits light of a specific wavelength at the photoelectric detection position 116 to detect the concentration of hemoglobin. The negative pressure port 18 provides negative pressure to exhaust the air in the box body 1. The liquid sample in the first cavity 12 passes through After the liquid sample passes through the microporous structure 3, it enters the second cavity 13, the first electrode 111 and the second electrode 112 are connected in the circuit, and a metering start signal is generated. After the liquid sample enters the third cavity 14 from the second cavity 13, the first electrode 111 and the third electrode 113 are in contact, and a metering termination signal is generated. The blood analyzer calculates and processes the optical signal and the electrical signal to obtain the particle detection result of the liquid sample. After the detection is completed, the liquid sample enters the fourth cavity 16 to prevent liquid leakage and contamination; the test kit is removed from the blood analyzer, and then the next test kit can be tested.

[0048] According to the disclosure and teaching of the above description, the technical personnel in the field of the utility model can also change and modify the above implementation. Therefore, the utility model is not limited to the specific implementation methods disclosed and described above, and some modifications and changes of the utility model should also fall within the scope of protection of the claims of the utility model. In addition, although some specific terms are used in this specification, these terms are only for the convenience of description and do not constitute any limitation to the utility model.

Claims

1. A reagent kit, comprising a box body (1) and a cover (2), wherein the cover (2) is arranged on the box body (1), characterized in that: The box body (1) is provided with a first cavity (12), a second cavity (13), a third cavity (14) and a microporous structure (3); the microporous structure (3) is arranged between the first cavity (12) and the second cavity (13); the second cavity (13) is connected to the third cavity (14).

2. The kit according to claim 1, characterized in that: A fourth cavity (16) is also provided in the box body (1), and the fourth cavity (16) is communicated with the third cavity (14).

3. The kit according to claim 2, characterized in that: The invention also comprises a first electrode (111), a second electrode (112) and a third electrode (113); the first electrode (111) penetrates the bottom wall of the first cavity (12) and is arranged in the first cavity (12); the second electrode (112) penetrates the bottom wall of the second cavity (13) and is arranged in the second cavity (13); and the third electrode (113) penetrates the bottom wall of the third cavity (14) and is arranged in the third cavity (14).

4. The kit according to claim 3, characterized in that: It also includes a negative pressure port (18), and the negative pressure port (18) is arranged in the fourth cavity (16).

5. The kit according to claim 4, characterized in that: It also includes an air guide column (181), which is arranged on the bottom wall of the fourth cavity (16), with part of the air guide column (181) being located in the fourth cavity (16) and close to the cover (2), and the other part protruding from the bottom surface of the box body (1).

6. The kit according to claim 1, characterized in that: The microporous structure (3) is a microporous sheet, a through groove (15) is provided between the first cavity (12) and the second cavity (13), and the microporous sheet is embedded in the through groove (15).

7. The kit according to claim 1, characterized in that: It also comprises a plug (5), the cover (2) is provided with a first through hole (21), the first through hole (21) is opposite to the first cavity (12), and the plug (5) is snap-connected to the first through hole (21).

8. The kit according to claim 1, characterized in that: It also comprises a side cover (4); a second through hole (11) is provided on the side wall of the box body (1); the second through hole (11) is communicated with the first cavity (12); and the side cover (4) is arranged on the second through hole (11).

9. The kit according to any one of claims 1 to 8, characterized in that: The box body (1) is also provided with a photoelectric detection position (116), and the photoelectric detection position (116) is arranged close to the first cavity (12).

10. A blood analyzer for POCT, characterized in that: A kit comprising any one of claims 1 to 9.

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