Movement device applied to full-automatic biochip spotting instrument

Abstract

The utility model relates to the technical field of medical instruments, and discloses a moving device applied to a full-automatic biochip spotting instrument, which comprises a moving platform arranged on a working platform through a first driving mechanism, and the first driving mechanism is suitable for driving the moving platform to move in the Y-axis direction by taking the end surface of the working platform as a reference; the sample application assembly comprises a base, a second driving mechanism, a third driving mechanism and a sample application module, the base is arranged on the working platform through the second driving mechanism, the second driving mechanism is suitable for driving the base to move in the X-axis direction, the sample application module is arranged on the base through the third driving mechanism, and the third driving mechanism is suitable for driving the sample application module to move in the Z-axis direction. Through cooperation of the first driving mechanism, the second driving mechanism and the third driving mechanism, alignment of the sample application module and the corresponding functional area is achieved, so that the alignment time is saved, the experiment efficiency is improved, manual participation is reduced, and the sample application precision and the sample application efficiency are improved.

Description

Technical Field

[0001] The utility model relates to the technical field of medical equipment, in particular to a motion device applied to a full-automatic biochip spotter. Background Art

[0002] As a pre-treatment process for nucleic acid mass spectrometry, chip spotting is to spot a trace amount of sample on the chip for use by the nucleic acid mass spectrometer. Currently, the spotting of most biochips relies on manual use of a pipette to manually transfer samples from the sample tray to the 96-well biochip. Manual pipetting can cause many problems such as inaccurate spotting and inconsistent spotting volume. This is not only labor-intensive, but also the sample may cause chip contamination due to improper operation during the manual transfer process, affecting the accuracy of mass spectrometry analysis. Utility Model Content

[0003] In view of this, the utility model provides a motion device applied to a fully automatic biochip spotter to solve the conventional problems.

[0004] The present application provides a motion device for a fully automatic biochip spotter, comprising:

[0005] A mobile platform is arranged on the working platform through a first driving mechanism, and the first driving mechanism is suitable for driving the mobile platform to move in the Y-axis direction with the end surface of the working platform as a reference;

[0006] The spotting component comprises a base, a second driving mechanism, a third driving mechanism and a spotting module, wherein the base is arranged on the working platform through the second driving mechanism, the second driving mechanism is suitable for driving the base to move in the X-axis direction, the spotting module is arranged on the base through the third driving mechanism, and the third driving mechanism is suitable for driving the spotting module to move in the Z-axis direction.

[0007] Optionally, the first driving mechanism comprises:

[0008] A first guide slider, arranged on the working platform;

[0009] A first slide rail, arranged at the bottom of the mobile platform, and cooperates with the guide groove on the first guide slider;

[0010] A first screw, fixedly arranged on the working platform;

[0011] The first driving motor is arranged on the mobile platform, and a first rotating member cooperating with the first lead screw is arranged in the first driving motor.

[0012] Optionally, the first lead screw extends along the Y-axis direction.

[0013] Optionally, a first roller and a second roller are provided on the edge of the mobile platform.

[0014] Optionally, a first micro switch is arranged on the moving path of the moving platform, and the first micro switch is fixedly arranged on the working platform.

[0015] Optionally, the second driving mechanism comprises:

[0016] A first bracket and a second bracket are respectively arranged on opposite sides of the working platform, and the mobile platform is located between the first bracket and the second bracket;

[0017] A second slide rail, one end of which is connected to the first bracket, and the other end of which is connected to the second bracket;

[0018] A second sliding block is fixedly arranged on the base, and the second sliding block has a sliding groove matched with the second sliding rail guide;

[0019] A second lead screw, one end of which is fixedly arranged on the first bracket, and the other end of which is fixedly arranged on the second bracket;

[0020] The second driving motor is arranged on the base, and a second rotating member matched with the second lead screw is arranged inside the second driving motor.

[0021] Optionally, the second slide rail is disposed between the first bracket and the second bracket and extends along the X-axis direction; the second lead screw is arranged between the first bracket and the second bracket along the extension direction of the X-axis.

[0022] Optionally, the first bracket is provided with a first guide groove, and the second bracket is provided with a second guide groove. The first guide groove and the second guide groove are arranged opposite to each other in the Y-axis direction and extend in the Z-axis direction. Both ends of the second slide rail are respectively located in the first guide groove and the second guide groove.

[0023] Optionally, the third driving mechanism comprises:

[0024] A third slide rail, disposed on the base;

[0025] A third sliding block is arranged on the spotting module, and the third sliding block has a sliding groove matched with the third sliding rail;

[0026] A third lead screw, fixedly disposed on the base;

[0027] The third driving motor is arranged on the spotting module, and a third rotating member cooperating with the third lead screw is arranged inside the third driving motor.

[0028] Optionally, a second micro switch (55) is provided on the base (3), and the second micro switch (55) is located on the moving path of the shell of the spotting module (4).

[0029] Beneficial effects:

[0030] The motion device for a fully automatic biochip spotter provided in the present application includes: a mobile platform, which is arranged on the working platform through a first driving mechanism, and the first driving structure is suitable for driving the mobile platform to move in the Y-axis direction with the end face of the working platform as a reference; a spotting assembly, which includes a base, a second driving mechanism, a third driving mechanism and a spotting module, wherein the base is arranged on the working platform through the second driving mechanism, the second driving mechanism is suitable for driving the base to move in the X-axis direction, and the spotting module is arranged on the base through the third driving mechanism, and the third driving mechanism is suitable for driving the spotting module to move in the Z-axis direction. The first driving mechanism is used to drive the mobile platform to move in the Y-axis direction, and the second driving mechanism and the third driving mechanism are used to drive the spotting module to move in the X-axis and Z-axis directions. Such cooperation can improve the alignment accuracy of the spotting module and the corresponding functional area, and the first driving mechanism and the second driving mechanism can work simultaneously, thereby saving alignment time, improving experimental efficiency, reducing manual participation, and reducing and improving spotting accuracy and efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] In order to more clearly illustrate the specific implementation methods of the utility model or the technical solutions in the prior art, the drawings required for use in the specific implementation methods or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are some implementation methods of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0032] Figure 1 This is a structural schematic diagram of a fully automatic biochip spotter according to an embodiment of the utility model;

[0033] Figure 2 for Figure 1 A top view of the fully automatic biochip spotter shown;

[0034] Figure 3 for Figure 1 A is a partial enlarged schematic diagram;

[0035] Figure 4 A schematic diagram of the structure of a cleaning pool according to an embodiment of the utility model;

[0036] Figure 5 This is a schematic structural diagram of a waste liquid pool according to an embodiment of the utility model;

[0037] Figure 6 for Figure 2A partial enlarged schematic diagram of B in the middle;

[0038] Figure 7 This is a schematic diagram of the structure of the cooling device of an embodiment of the utility model;

[0039] Figure 8 It is a structural schematic diagram of the injection pump assembly of an embodiment of the utility model;

[0040] Fig. 9 A schematic diagram of the structure of a mobile platform according to an embodiment of the utility model;

[0041] Fig.10 This is a schematic structural diagram of a first driving mechanism according to an embodiment of the utility model;

[0042] Fig.11 It is a structural schematic diagram of the second driving mechanism of an embodiment of the utility model;

[0043] Fig.12 This is a schematic structural diagram of a third driving mechanism of an embodiment of the utility model;

[0044] Fig.13 It is a structural schematic diagram of the third driving mechanism of the embodiment of the utility model from another angle;

[0045] Fig.14 It is a top view of the third driving mechanism of the embodiment of the utility model.

[0046] Description of reference numerals:

[0047] 1. working platform; 101. display screen; 102. power supply; 103. drag chain; 2. mobile platform; 211. first guide slider; 212. first slide rail; 22. first lead screw; 221. first fixed seat; 222. second fixed seat; 23. first drive motor; 241. first roller; 242. second roller; 25. first micro switch; 3. base; 31. first bracket; 311. first guide groove; 312. first threaded hole; 313. third threaded hole; 32. second bracket Frame; 321, second guide groove; 322, second threaded hole; 323, fourth threaded hole; 331, second slide rail; 332, second slider; 34, second lead screw; 35, second drive motor; 4, spotting module; 41, spotting needle; 42, collection device; 421, mounting base; 5, sample area; 501, sample base; 502, sample well plate; 51, third slide rail; 52, third slider; 53, third lead screw; 54, third drive motor; 551, first support; 552, third Second support; 55, second micro switch; 531, heat conduction plate; 532, cold water head; 533, coolant bottle; 534, water cooling pump; 535, cooling fan; 6, chip spotting area; 60, chip target plate; 611, chip base; 612, eccentric hole; 62, chip placement area; 63, standard product area; 7, cleaning pool; 701, resin box; 702, cover plate; 71, first cleaning area; 711, first bottom surface; 712, second bottom surface; 713, third bottom surface; 714, liquid hole; 7 2. Second cleaning area; 721. Opening; 722. Flow ramp; 73. Waste liquid pool; 74. Waste liquid bottle; 75. Diaphragm pump; 761. First pipeline; 762. Second pipeline; 8. Injection pump assembly; 81. Frame; 811. Limiting boss; 82. Servo motor for pump; 821. Lead screw for pump; 83. Piston tube; 84. First mounting plate; 85. Second mounting plate; 861. Guide rail for pump; 862. Slider for pump; 87. Solenoid valve; 88. Displacement sensor; 89. Liquid container. DETAILED DESCRIPTION

[0048] In order to make the purpose, technical solution and advantages of the embodiment of the utility model clearer, the technical solution in the embodiment of the utility model will be clearly and completely described below in conjunction with the drawings in the embodiment of the utility model. Obviously, the described embodiment is a part of the embodiment of the utility model, not all the embodiments. Based on the embodiment of the utility model, all other embodiments obtained by those skilled in the art without creative work are within the scope of protection of the utility model.

[0049] like Figure 1 and Figure 2As shown, in this embodiment, a fully automatic biochip spotter is provided, including: a working platform 1, a moving platform 2, a spotting component, a sample area 5, a chip spotting area 6, a cleaning pool 7 and a resin box 701.

[0050] The mobile platform 2 is arranged on the working platform 1 through a first driving mechanism. Taking the end surface of the working platform 1 as a reference, the first driving structure is suitable for driving the mobile platform 2 to move in the Y-axis direction.

[0051] The spotting assembly includes a base 3, a second driving mechanism, a third driving mechanism and a spotting module 4. The base 3 is arranged on the working platform 1 through the second driving mechanism, and the second driving mechanism is suitable for driving the base 3 to move in the X-axis direction. The spotting module 4 is arranged on the base 3 through the third driving mechanism, and the third driving mechanism is suitable for driving the spotting module 4 to move in the Z-axis direction.

[0052] The sample area 5 is arranged on the mobile platform 2, and has a plurality of sample points arranged in a matrix. The chip spotting area 6 is arranged on the mobile platform 2, and the cleaning pool 7 is arranged on the mobile platform 2. The sample area 5, the chip spotting area 6 and the cleaning pool 7 are all located on the mobile platform 2. When the first driving mechanism drives the mobile platform 2 to move, the three can be driven to move synchronously. The resin box 701 is arranged on the working platform 1.

[0053] During operation, the first driving mechanism can drive the mobile platform 2 to move along the Y-axis direction on the working platform 1, the second driving mechanism can drive the base 3 to move in the X-axis direction, and the third driving mechanism can drive the spotting module 4 to move in the Z-axis direction. The first driving mechanism, the second driving mechanism and the third driving mechanism can realize the movement of the spotting module 4 along the X-axis, the Y-axis and the Z-axis in the spatial coordinates, so that the spotting module 4 can be accurately moved to the corresponding position.

[0054] Since the sample needs to be desalted, the second driving mechanism can drive the spotting module 4 to move along the X-axis to the top of the resin box 701. After the third driving mechanism drives the spotting module 4 to move and descend along the Z-axis to the position, the spotting needle 41 of the spotting module 4 can absorb the resin in the resin box 701. After the resin absorption is completed, the third driving mechanism drives the spotting module 4 to rise along the Z-axis until the spotting needle 41 leaves the resin box 701. The second driving mechanism drives the spotting module 4 to move along the X-axis to a position corresponding to the spotting needle 41 and the sample point in the Y-axis direction. The first driving mechanism can drive the mobile platform 2 to move along the Y-axis direction until the sample point to be desalted corresponds to the spotting needle 41 in the Z-axis direction. After the mobile platform 2 moves to the position, the third driving mechanism can drive the spotting needle 41 to descend along the Z-axis direction to correspond to the sample point. Thereafter, the spotting needle 41 can repeatedly suck and spit in the sample point to desalinate the sample. After the corresponding sample desalting treatment is completed, the third driving mechanism can drive the sample spotting needle 41 to rise along the Z axis direction to leave the sample point, the second driving mechanism drives the sample spotting module 4 to move along the X axis to the position corresponding to the cleaning pool 7 in the Y axis direction, the first driving mechanism drives the mobile platform 2 to move along the Y axis to the cleaning pool 7 and the sample spotting needle 41 of the sample spotting module 4 in the Z axis direction, the third driving mechanism drives the sample spotting module 4 to descend along the Z axis until the sample spotting needle 41 corresponds to the cleaning pool 7, the sample spotting needle 41 can be cleaned in the cleaning pool 7, after the cleaning is completed, the third driving mechanism drives the sample spotting module 4 to rise along the Z axis until the sample spotting needle 41 leaves the cleaning pool 7. The above steps can be repeated to desalt each sample.

[0055] After the desalting step is completed, the biochip can be spotted. The first driving mechanism can drive the mobile platform 2 to move along the Y-axis direction until the sample point is aligned with the spotting module 4 in the X-axis direction. The second driving mechanism drives the spotting module 4 to move along the X-axis direction until the spotting needle 41 of the spotting module 4 is aligned with the sample point in the Z-axis direction. The third driving mechanism drives the spotting module 4 to descend along the Z-axis direction until the spotting needle 41 corresponds to the sample point. After the spotting needle 41 absorbs the sample, the third driving mechanism drives the spotting module 4 to rise along the Z-axis direction until the spotting needle 41 is separated from the sample point. The second driving mechanism drives the spotting module 4 to move along the X-axis until the spotting needle 41 is aligned with the chip spotting area 6 in the Y-axis direction. After that, the first driving mechanism can drive the mobile platform 2 to move along the Y-axis direction to the chip spotting area The chip sampling area 6 is aligned with the sample needle 41 in the Z-axis direction, and the third driving mechanism drives the sample needle 41 to descend along the Z-axis direction to correspond to the chip sampling area 6. After the sample needle 41 completes the sampling, the third driving mechanism drives the sample needle 41 to rise along the Z-axis direction to leave the chip sampling area 6. The second driving mechanism drives the sample module 4 to move along the X-axis to a position corresponding to the cleaning pool 7 in the Y-axis direction. The first driving mechanism drives the mobile platform 2 to move along the Y-axis to the cleaning pool 7 and the sample needle 41 of the sample module 4 in the Z-axis direction. The third driving mechanism drives the sample module 4 to descend along the Z-axis to correspond to the sample needle 41 in the cleaning pool 7. The sample needle 41 can be cleaned in the cleaning pool 7. After the cleaning is completed, the third driving mechanism drives the sample module 4 to rise along the Z-axis until the sample needle 41 leaves the cleaning pool 7. The above steps can be repeated to absorb the sample and spot the sample in the chip sampling area 6.

[0056] The fully automatic biochip spotter provided by the present invention can realize the functions of desalting, cleaning and spotting, not only replacing manual spotting and manual cleaning, but also being able to implement desalting before spotting, and being able to completely complete the necessary steps before and during mass spectrometry spotting, thereby reducing manual participation, improving the automation of biochip spotting, improving spotting accuracy and spotting efficiency, and improving experimental efficiency.

[0057] like Figure 1As shown, in this embodiment, the chip spotting area 6 and the cleaning pool 7 are respectively arranged near two adjacent edges of the sample area 5. For example, the chip spotting area 6 can be arranged in the X-axis direction of the sample area 5, and the cleaning pool 7 can be arranged in the Y-axis direction of the sample area 5. An L-shaped moving space allowing the spotting module 4 to move is formed at the adjacent edges of the sample area 5. After the spotting needle 41 of the spotting module 4 completes the spotting work on the chip spotting area 6, the first driving mechanism can drive the moving platform 2 to move along the Y-axis until the spotting needle 41 of the spotting module 4 is aligned with the cleaning pool 7 in the X-axis direction, the second driving mechanism drives the base 3 to move in the Y-axis direction until the spotting needle 41 of the spotting module 4 is aligned with the cleaning pool 7 in the Z-axis direction, and the third driving mechanism drives the spotting module 4 to descend along the Z-axis direction until the spotting needle 41 enters the cleaning pool 7. In this process, the spotting module 4 can move along the L-shaped moving space without passing over the sample area 5, thereby preventing the residual trace sample in the spotting needle 41 from dripping during the movement process, affecting the cleanliness of the sample area 5.

[0058] The sample area 5 can be arranged on the end face of the mobile platform 2 close to the resin box 701, and the cleaning pool 7 is also arranged close to the resin box 701. Such a layout is conducive to the efficient reciprocating movement of the sample spotting module 4 between the resin box 701, the sample area 5 and the cleaning pool 7, thereby completing the desalting work of all samples, reducing the moving stroke of the sample spotting module 4, and improving the desalting efficiency. At the same time, during the desalting process, the sample spotting module 4 will not pass over the chip sample spotting area 6, so as to avoid the residual liquid in the sample spotting needle 41 of the sample spotting module 4 from accidentally dripping into the sample area 5. The bottom wall of the resin box 701 has an inclined surface and a bottom plane. The bottom plane is mainly used to accommodate resin. The sample spotting needle 41 can move to the bottom plane to absorb resin to ensure that the sample spotting needle 41 can absorb a sufficient amount of resin. A liquid level sensor can be arranged in the resin box 701. The liquid level sensor can detect the corresponding position of the inclined surface. When the liquid level height is lower than the preset value, the liquid level sensor is triggered to remind the staff to replenish the resin.

[0059] like Figure 1 and Figure 2 As shown, in this embodiment, the spotting module 4 is provided with a cover plate 702 of the same size as the top opening of the resin box 701, and the top opening of the resin box 701 is located on the moving path of the cover plate 702 in the X-axis direction. After the spotting is completed, the third driving mechanism drives the spotting module 4 to rise along the Z-axis direction to leave the chip spotting area 6, and the second driving mechanism drives the spotting module 4 to move along the X-axis direction until the cover plate 702 and the top opening of the resin box 701 are aligned in the Z-axis direction, and then the third driving mechanism drives the spotting module 4 to descend along the Z-axis direction until the cover plate 702 is buckled on the top opening of the resin box 701. In this way, the sealing of the resin box 701 is completed to prevent external impurities from entering the resin box 701 and causing pollution during non-working hours.

[0060] like Figure 1 and Figure 3 As shown, in this embodiment, the spotting module 4 includes a plurality of spotting needles 41 and a collection device 42. For example, six spotting needles 41 are arranged in a 2*3 matrix on the spotting module 4. The spotting needles 41 extend along the Z-axis direction. The spotting needles 41 have internal channels and needle ports opened downward. The outer surface coating of the spotting needles 41 is Teflon. Such a setting can increase the smoothness of the outer surface of the spotting needles 41 and prevent liquid from remaining on the outer wall of the spotting needles 41 during the cleaning and spotting process. The collection device 42 can be a camera. The collection device 42 collects images of the droplet state of the spotting needles 41 after the spotting is completed, so as to facilitate the staff to confirm whether the spotting position and the spotting amount are correct. The spotting module 4 is provided with a mounting substrate 421 for the collection device 42.

[0061] For example, after the spotting is completed, the third driving mechanism drives the acquisition device 42 to rise along the Z-axis direction to the chip spotting area 6. When it is necessary to collect images of one or more targets, the first driving mechanism drives the mobile platform 2 to move in the Y-axis direction until the target and the acquisition device 42 are aligned in the X-axis direction. The second mechanism moves and drives the acquisition device 42 to move along the X-axis direction to align with the target. The third driving mechanism can drive the acquisition device 42 to move along the Z-axis direction so that the acquisition device 42 is focused on the target. After that, the acquisition device 42 can take pictures and process images, and can also be used to observe the spotting effect in real time. The taken images will be stored and transmitted to the control unit, and the control unit will automatically calculate the volume for subsequent retrieval and viewing. In actual applications, an image acquisition can be performed after completing a spotting, so as to observe the spotting effect in a timely manner. After observation, subsequent spotting is performed.

[0062] like Figure 3 and Figure 4As shown, in this embodiment, the cleaning pool 7 includes a first cleaning area 71 and a second cleaning area 72. The first cleaning area 71 is a groove, and the second cleaning area 72 includes openings 721 corresponding to the position and number of the spotting needles 41 of the spotting module. An overflow slope 722 is arranged between the second cleaning area 72 and the first cleaning area 71. When cleaning the spotting needle 41, the spotting needle 41 first spit out liquid in the first cleaning area 71, thereby cleaning the internal channel of the spotting needle 41, and then moves to the second cleaning area 72 and extends into the opening 721. For example, the second cleaning area 72 is provided with six openings 721, and the six openings 721 correspond to the six spotting needles 41 in position. After the spotting needle 41 is inserted into place, it starts to spit out liquid, and the liquid rises between the liquid levels in the openings 721, thereby flowing through the outer wall of the spotting needle 41, thereby cleaning the outer wall of the spotting needle 41, and the overflowed liquid will flow into the flow slope 722 outside the opening 721, and the liquid flows into the first cleaning area 71 from the flow slope 722, thereby completing the cleaning of the internal channel and outer wall of the spotting needle 41, ensuring that the spotting needle 41 is in a clean state for the next spotting or desalting.

[0063] like Figure 4 As shown, in this embodiment, the first cleaning area 71 includes a first bottom surface 711, a second bottom surface 712 and a third bottom surface 713 of decreasing heights in sequence. The first bottom surface 711 is an inclined surface inclined downwardly toward the third bottom surface 713, and the second bottom surface 712 is an inclined surface inclined downwardly toward the third bottom surface 713. The spotting needle 41 can complete the liquid discharge on the first bottom surface 711, and the liquid will flow to the third bottom surface 713 under the guidance of the first bottom surface 711, so that the liquid surface of the first cleaning area 71 contacts the outer wall of the spotting needle 41.

[0064] like Figure 1 and Figure 4 As shown, in this embodiment, a liquid hole 714 is provided on the side wall of the first cleaning area 71 away from the second cleaning area 72, and a waste liquid pool 73 is provided on the working platform, and the waste liquid pool 73 is connected to the liquid hole 714 through a waste liquid pipe. The liquid hole 714 can be opened on the side wall close to the third bottom surface 713. When the spotting needle 41 spits liquid, the liquid enters the third bottom surface 713 and can be discharged from the liquid hole 714 into the waste liquid pool 73. When there is a lot of liquid, a small amount of liquid will enter the second bottom surface 712, and the second bottom surface 712 can buffer part of the waste liquid. As the waste liquid is continuously discharged from the liquid hole 714, the liquid on the second bottom surface 712 will flow into the third bottom surface 713 again, and then be discharged through the liquid hole 714, thereby preventing the waste liquid from entering the first bottom surface 711 and contacting the spotting needle 41.

[0065] like Figure 5As shown, in the present embodiment, a buffer structure is arranged between the cleaning tank 7 and the waste liquid tank 73, the buffer structure includes a waste liquid bottle 74 and a diaphragm pump 75, the waste liquid pipe includes a first pipeline 761 and a second pipeline 762, the waste liquid bottle 74 has a first opening and a second opening, the first pipeline is connected to the liquid hole 714 and the waste liquid bottle 74, and the first pipeline 761 extends into the first opening, the pipe mouth of the first pipeline 761 is close to the first opening, one end of the second pipeline 762 extends into the second opening, and the other end is connected to the waste liquid tank, the diaphragm pump 75 is arranged on the second pipeline 762, and the pipe mouth of the second pipeline 762 extending into the second opening is far away from the second opening. With such arrangement, when there is waste liquid in the cleaning tank 7, the waste liquid can directly enter the waste liquid bottle 74 through the liquid hole 714, the first pipeline 761, and the first opening, thereby buffering the waste liquid. The diaphragm pump 75 can pump the waste liquid in the waste liquid bottle 74 into the waste liquid tank 73 through the second pipeline 762. When a large amount of waste liquid is stored in the waste liquid tank 73, the waste liquid can be temporarily buffered through the waste liquid bottle 74, thereby providing time for the drainage of the waste liquid tank 73.

[0066] In this embodiment, both the cleaning tank 7 and the waste liquid tank 73 are provided with liquid level sensors to facilitate detection of waste water capacity. The liquid passage hole 714 of the cleaning tank 7 and the water inlet hole of the waste liquid tank 73 are provided with quick-release joints. After the waste liquid pipe is pulled out from the liquid passage hole 714 or the water inlet hole, the quick-release joint can prevent the waste liquid from flowing out, so as to facilitate replacement of the cleaning tank 7 or the waste liquid tank 73. A drain port can be provided below the waste liquid tank 73, and the drain port is provided with a quick-release joint, which can be connected to the drain port through an external S pipeline to quickly discharge the waste liquid.

[0067] like Figure 2 and Figure 3 In this embodiment, the sample area 5 includes a sample base 501 and a sample well plate 502 placed on the sample base 501. For example, the sample well plate 502 has ninety-six sample points, and the ninety-six sample points are arranged in an 8*12 matrix. The sample area 5 can be provided with two sample bases 501 for placing two sample well plates 502 at the same time. A refrigeration device is provided below the sample base 501, and the refrigeration device can provide coldness to the sample base 501, so as to preserve the sample placed in the sample well plate 502 on the sample base 501.

[0068] like Figure 7 As shown, the refrigeration device includes a heat conducting plate 531 and a cold water head 532 arranged below the sample base 501, and uses water cooling to cool the heat conducting plate 531, thereby cooling the sample base 501. The cold water head 532 is connected to a coolant bottle 533 and a water cooling pump 534 through a refrigeration circulation pipeline, and a cold fan 535 is arranged on the water cooling pump 534. A temperature sensor is arranged on the refrigeration device, and the temperature sensor is used to detect the temperature of the sample base 501, so as to adjust the cooling temperature and ensure that the sample is kept at the optimal fresh-keeping temperature.

[0069] like Figure 2 and Figure 6 As shown, in this embodiment, the chip spotting area 6 includes a chip base 611 and a chip target plate 60 placed on the chip base 611. The chip target plate 60 has ninety-six sample target points, and the ninety-six sample target points can be arranged in an 8*12 matrix. The chip base 611 is provided with an eccentric hole 612, and the eccentric hole 612 is arranged away from the middle of the chip base 611. The back of the chip target plate 60 is provided with a magnetic suction piece corresponding to the position of the eccentric hole 612. The chip base 611 has a ring frame corresponding to the shape of the outer edge of the chip target plate 60. The chip target plate 60 can be embedded and installed in the ring frame. The eccentric hole 612 of the chip base 611 and the magnetic suction piece on the back of the chip target plate 60 can facilitate the alignment and fixation of the chip target plate 60. A notch 613 is provided on the chip base 611, and the notch 613 runs through the ring frame. In this way, the chip target plate 60 can be taken out through the notch 613.

[0070] A heating device is provided under the chip base 611, and the heating device can heat the chip base 611, and then heat the chip target plate 60, so there is no need to remove the chip target plate 60 and use other devices to heat it, which speeds up the subsequent observation process. A temperature sensor is provided on the heating device, and the temperature sensor is used to detect the temperature of the chip base 611, so as to adjust the heating temperature.

[0071] like Figure 6 As shown, in this embodiment, the chip spotting area 6 is provided with two chip bases 611 , and the two chip bases 611 have the same structure and can place the chip target plate 60 at the same time.

[0072] In this embodiment, a chip placement area 62 and a standard product area 63 are provided near the chip spotting area 6 on the mobile platform 2. The chip placement area 62 is also provided with a chip base 611, and the chip placement area 62 is used to temporarily place the chip target plate 60. The standard product area 63 has spot holes corresponding to the number and position of the spotting needles 41 and a separate spot hole. The standard product area 63 can prevent standard samples, and the spotting needles 41 can spot standard samples to test the spotting effect.

[0073] like Figure 2 As shown, in this embodiment, the working platform 1 is provided with a syringe pump assembly 8, which is connected to the sample spotting needles 41 of the sample spotting module 4. The syringe pump assembly 8 has pump liquid tubes respectively connected to the internal channels of the plurality of sample spotting needles 41, so that the plurality of sample spotting needles 41 can selectively suck and discharge liquid.

[0074] Specifically, Figure 8As shown, the injection pump assembly 8 includes a frame 81, a pump servo motor 82, a piston tube 83, a first mounting plate 84, a second mounting plate 85, a pump screw 821, a pump guide rail 861, a pump slider 862, a solenoid valve 87, a displacement sensor 88, and a limit boss 811. The frame 81 is installed on the working platform 1, the pump guide rail 861 is installed on the frame 81, the second mounting plate 85 is slidably matched with the pump guide rail 861 through the pump slider 862, the pump servo motor 82 is arranged on the second mounting plate 85, and is transmitted and matched with the pump lead screw 821 arranged between the frame 81 and the first mounting plate 84, the first mounting plate 84 and the second mounting plate 85 are arranged opposite to each other, the piston tube 83 is arranged between the first mounting plate 84 and the second mounting plate 85, the solenoid valve 87 is arranged on the first mounting plate 84 and communicated with the piston tube 83, the solenoid valve 87 is connected with the internal channel of the sample spotting needle 41 through the liquid supply pipeline, and there are six solenoid valves 87 and piston tubes 83, which are respectively connected with the corresponding sample spotting needles 41 through the liquid supply pipeline. When the pump servo motor 82 is working, it can drive the second mounting plate 85 to reciprocate along the extension direction of the pump lead screw 821, and cooperate with the first mounting plate 84 to drive the piston tube 83 to do piston movement, so as to provide positive pressure or negative pressure to the liquid supply pipeline to control the sample needle 41 to absorb or discharge liquid. The displacement sensor 88 is arranged on the end surface of the second mounting plate 85 facing the first mounting plate 84. The first mounting plate 84 is provided with a limit boss 811 corresponding to the moving path of the displacement sensor 88. When the displacement sensor 88 moves to contact the limit boss 811, the displacement sensor 88 is triggered to indicate that the second mounting plate 85 has moved to the limit position, the pump servo motor 82 stops working or reverses, and the displacement sensor 88 is used to indicate the moving origin.

[0075] For example, when the spotting needle 41 needs to absorb liquid, the pump servo motor 82 drives the second mounting plate 85 to move away from the first mounting plate 84. When the spotting needle 41 needs to discharge liquid, the pump servo motor 82 drives the second mounting plate 85 to move toward the first mounting plate 84. The pump servo motor 82 is provided with a nut that rotates with the pump lead screw 821. The nut is fixedly connected to the rotor of the pump servo motor 82, so that the pump servo motor 82 can accurately control the amount of liquid absorbed and discharged. Compared with the traditional servo motor driving the lead screw to rotate, such a setting is conducive to saving layout space.

[0076] In this embodiment, a liquid container 89 is provided on the working platform 1, and the injection pump assembly 8 is connected to the liquid container 89 through a liquid tube. The injection pump assembly 8 is suitable for selectively sucking liquid in the liquid container 89 through the liquid tube. The injection pump assembly is provided with a two-position three-way solenoid valve. The solenoid valve 87 is a two-position three-way solenoid valve. The normally open channel of the two-position three-way solenoid valve is connected to the liquid inlet and outlet of the injection pump assembly 8, that is, it is connected to the piston tube 83. The two selection channels of the two-position three-way solenoid valve are respectively connected to the spotting needle 41 and the liquid tube. For example, when the spotting needle 41 needs to suck or discharge a sample, the channel corresponding to the spotting needle 41 can be opened, so that the piston tube 83 can be used to control the spotting needle 41 to suck or discharge liquid. When the spotting needle 41 needs to be cleaned, the selection channel connected to the two-position three-way solenoid valve and the liquid tube can be opened first, and the piston tube 83 can be used to suck liquid in the liquid container through the liquid tube. Then, the channel corresponding to the spotting needle 41 can be opened, and the liquid in the piston tube 83 can be transported to the spotting needle 41 through the liquid supply pipeline to clean the internal channel and outer wall of the spotting needle 41.

[0077] like Figure 1 As shown, in this embodiment, the working platform 1 has a first layer and a second layer, and a support plate is provided between the first layer and the second layer. The support plate supports the first layer to have a certain distance from the second layer, and the waste liquid pool 73 and the liquid container 89 can be arranged between the first layer and the second layer, thereby improving the compactness of the structure. The mobile platform 2 can be movably arranged on the first layer, and other non-movable structural parts can be arranged on the second layer, thereby improving the space utilization rate.

[0078] like Figure 1 As shown, in this embodiment, a drag chain 103 is provided on the working platform 1, and the drag chain 103 is used for wiring.

[0079] like Fig. 9 and Fig.10 As shown, in this embodiment, the first driving mechanism includes:

[0080] The first guide slider 211 is disposed on the working platform 1 . The first slide rail 212 is disposed at the bottom of the moving platform 2 and cooperates with the guide groove on the first guide slider 211 .

[0081] The first lead screw 22 is fixedly arranged on the working platform 1. The working platform 1 may be provided with a first fixed seat 221 and a second fixed seat 222. The two ends of the first lead screw 22 are respectively connected to the first fixed seat 221 and the second fixed seat 222. The first lead screw 22 extends along the Y-axis direction. The first drive motor 23 is arranged on the mobile platform 2. The first drive motor 23 may be fixedly connected to the edge of the mobile platform 2. A first rotating member cooperating with the first lead screw 22 is arranged in the first drive motor 23. The first lead screw 22 is inserted in the first drive motor 23 and cooperates with the first rotating member. The first transmission member may be a nut. The nut is fixedly connected to the rotation of the first drive motor 23. When the first drive motor 23 is working, the nut rotates with the rotor, and drives the first drive motor 23 to move along the Y-axis direction through the internal thread and the external thread of the first lead screw 22, thereby driving the mobile platform 2 to move along the Y-axis direction. Compared with the conventional servo motor driving the lead screw to rotate and drive the nut to move, such a setting can improve the accuracy of the first drive motor 23 driving the mobile platform 2 to move.

[0082] During the movement of the first driving motor 23 , the first guide slider 211 and the first slide rail 212 support and guide the mobile platform 2 , so that the mobile platform 2 moves stably along the Y-axis direction.

[0083] like Fig. 9 and Fig.10 As shown, in this embodiment, a first roller 241 and a second roller 242 are arranged at the edge of the mobile platform 2, and the first roller 241 and the second roller 242 are respectively arranged on one of the two diagonal edges of the mobile platform 2, and the first roller 241 and the second roller 242 are rotatably matched with the working platform 1 to assist the first guide slider 211 and the first slide rail 212 to jointly support the mobile platform 2 to move relative to the working platform 1 along the Y-axis direction. Specifically, the second roller 242 is arranged at the edge of the mobile platform 2 close to the cleaning pool 7, so as not to interfere with other structural parts arranged at the edge of the mobile platform 2, for example, not to interfere with the arrangement of the resin box 701, so that the working platform 1 becomes compact as a whole.

[0084] like Fig.10 As shown, in this embodiment, a first micro switch 25 is provided on the moving path of the mobile platform 2, and the first micro switch 25 is fixedly provided on the working platform 1. The first micro switch 25 is used to indicate the extreme moving position of the mobile platform 2. When the mobile platform 2 moves to trigger the first micro switch 25, the first drive motor 23 stops working. For example, when the mobile platform 2 moves along the Y axis to contact the first micro switch 25, the first micro switch 25 is triggered, and the first drive motor 23 stops working.

[0085] like Figure 1 , Fig.11 and Fig.12As shown, in this embodiment, the second driving mechanism includes:

[0086] The first bracket 31 and the second bracket 32 ​​are respectively arranged on opposite sides of the working platform 1, and the mobile platform 2 is located between the first bracket 31 and the second bracket 32. One end of the second slide rail 331 is connected to the first bracket 31, and the other end is connected to the second bracket 32. The second slider 332 is fixedly arranged on the base 3, and the second slider 332 has a slide groove that cooperates with the second slide rail 331. One end of the second lead screw 34 is fixedly arranged on the first bracket 31, and the other end is fixedly arranged on the second bracket 32. The second drive motor 35 is arranged on the base 3, and a second rotating member that cooperates with the second lead screw 34 is arranged in the second drive motor 35.

[0087] The first bracket 31 and the second bracket 32 ​​support the base 3 to be a certain distance away from the mobile platform 2, so as to facilitate the movement of the spotting component along the X-axis and the Z-axis. The mobile platform 2 is located between the first bracket 31 and the second bracket 32. The mobile platform 2 can be driven by the first driving mechanism to move along the Y-axis direction to facilitate the cooperation with the spotting component.

[0088] Specifically, the second slide rail 331 is arranged between the first bracket 31 and the second bracket 32, and extends along the X-axis direction. Similarly, the second lead screw 34 is also arranged between the first bracket 31 and the second bracket 32 ​​along the extension direction of the X-axis. The base 3 is guided and matched with the second slide rail 331 through the second slider 332. The second drive motor 35 is matched with the second lead screw 34 through the second transmission member. The second transmission member can be a nut. The nut is fixedly connected to the rotor of the second drive motor 35. When the second drive motor 35 is working, the nut can be driven to rotate by the rotor. The internal thread of the nut is matched with the external thread of the second lead screw 34. The second drive motor 35 can drive the base 3 to move along the X-axis. The second slider 332 and the second slide rail 331 cooperate to make the base 3 move more smoothly.

[0089] like Fig.11 and Fig.12 As shown, in this embodiment, the first bracket 31 is provided with a first guide groove 311, and the second bracket 32 ​​is provided with a second guide groove 321. The first guide groove 311 and the second guide groove 321 are relatively arranged in the Y-axis direction and extend in the Z-axis direction. The two ends of the second slide rail 331 are respectively located in the first guide groove 311 and the second guide groove 321. The first guide groove 311 and the second guide groove 321 can facilitate the installation of the second slide rail 331 and can quickly perform leveling and alignment in the Y-axis direction.

[0090] like Fig.11 and Fig.12As shown, in this embodiment, the first bracket 31 is provided with a first threaded hole 312, and the second bracket 32 ​​is provided with a second threaded hole 322. Correspondingly, corresponding threaded holes are respectively provided at both ends of the second slide rail 331. The fastening screws pass through the threaded holes at one end of the first threaded hole 312 and the second slide rail 331, and pass through the threaded holes at the other end of the second threaded hole 322 and the second slide rail 331, respectively, so as to fix the second slide rail 331 between the first bracket 31 and the second bracket 32. Two first threaded holes 312 can be provided along the Z-axis direction, and two second threaded holes 322 can be provided along the Z-axis direction. Correspondingly, two threaded holes are respectively provided at both ends of the second slide rail 331, so that the second slide rail 331 is limited by the first threaded hole 312 and the second threaded hole 322 to prevent the second slide rail 331 from rotating, and at the same time, the connection strength between the second slide rail 331 and the first bracket 31 and the second bracket 32 ​​can be increased.

[0091] like Fig.11 and Fig.12 As shown, in this embodiment, a third threaded hole 313 is provided on the first bracket 31, and a fourth threaded hole 323 is provided on the second bracket 32. The third threaded hole 313 and the fourth threaded hole 323 are arranged relatively to each other in the X-axis direction. Threaded holes are provided at both ends of the second lead screw 34, and the second lead screw 34 can be fixed between the first bracket 31 and the second bracket 32 ​​by tightening screws.

[0092] The base 3 can be accurately moved along the X-axis direction by the guiding effect of the second lead screw 34 , the second slide rail 331 and the second slider 332 arranged along the X-axis direction.

[0093] like Fig.10 , Fig.11 and Fig.13 As shown, in this embodiment, the third driving mechanism includes:

[0094] The third slide rail 51 is arranged on the base 3. The third slider 52 is arranged on the sample spotting module 4, and the third slider 52 has a slide groove matched with the third slide rail 51. The third lead screw 53 is fixedly arranged on the base 3. The third driving motor 54 is arranged on the sample spotting module 4, and a third rotating member matched with the third lead screw 53 is arranged in the third driving motor 54. The third transmission member can be a nut, and the nut is fixedly connected with the rotor of the third driving motor 54. When the third driving motor 54 is working, the rotor drives the nut to rotate, and the internal thread of the nut rotates and matches with the external thread of the third lead screw 53. The third driving motor 54 drives the sample spotting module 4 to move along the third lead screw 53 in the Z-axis direction. Such a setting is conducive to improving the movement accuracy of the sample spotting module 4.

[0095] The third sliding block 52 and the third sliding rail 51 are slidably matched, so that the spotting module 4 can move stably along the Z-axis direction.

[0096] Specifically, if Fig.13 and Fig.14 As shown, the first support 551 and the second support 552 are relatively arranged on the base 3 along the Z-axis direction, and the two ends of the third lead screw 53 are respectively fixed on the first support 551 and the second support 552, and the third lead screw 53 extends along the Z-axis direction, so that the third driving motor 54 can drive the spotting module 4 to move accurately along the Z-axis direction.

[0097] Similarly, the third slide rail 51 is arranged on the base 3 along the Z-axis direction, and the slide groove of the third slider 52 is arranged on the spotting module 4 along the Z-axis direction. The cooperation between the slide grooves of the third slide rail 51 and the third slider 52 further improves the stability of the spotting module 4 moving along the Z-axis direction.

[0098] like Fig.10 As shown, in this embodiment, a second micro switch 55 is provided on the base 3, and the second micro switch 55 is located on the moving path of the shell of the spotting module 4. When the spotting module 4 moves to contact with the second micro switch 55, the third driving motor 54 stops working. The second micro switch 55 is used to indicate the extreme rising position of the spotting module 4, and can also be used to indicate the movement origin of the spotting module 4.

[0099] The first drive mechanism, the second drive mechanism and the third drive mechanism can respectively drive the corresponding components to move, so as to realize the accurate alignment of the spotting module 4 with the corresponding functional area in the spatial coordinates. The first drive mechanism can drive the mobile platform 2 to move in the Y-axis direction to the corresponding functional area and align with the spotting module 4 on the X-axis, and the second drive mechanism can drive the spotting module 4 to move along the X-axis direction to the corresponding functional area in the Y-axis direction, so that the spotting module 4 can be aligned with the corresponding functional area in the Z-axis direction. The third drive mechanism can drive the spotting needle 41 of the spotting module 4 to rise or fall along the Z-axis direction, so that the spotting needle 41 can achieve liquid suction and discharge in the corresponding functional area. By using the mobile platform 2 to move in the Y-axis direction and the spotting module 4 to move in the X-axis and Z-axis directions, such coordination can improve the alignment accuracy of the spotting module 4 and the corresponding functional area. The first drive mechanism and the second drive mechanism can work simultaneously, thereby saving alignment time and improving experimental efficiency. Specifically, the first drive motor 23 and the first lead screw 22 cooperate to drive the mobile platform to move in the Y-axis direction, the second drive motor 35 and the second lead screw 34 cooperate to drive the spotting module 4 to move in the X-axis direction, and the third drive motor 54 and the third lead screw 53 cooperate to drive the spotting module 4 to move in the Z-axis direction.

[0100] The rotation speed and direction of the first drive motor 23, the second drive motor 35 and the third drive motor 54 can be controlled by a control unit. Specifically, the working platform 1 is provided with functional devices such as a PCB board 101, a power supply 102, a main board, a barcode scanner and a display screen. The staff can observe the working status of the biochip spotter through the display screen.

[0101] Although the embodiments of the present invention have been described in conjunction with the accompanying drawings, those skilled in the art may make various modifications and variations without departing from the spirit and scope of the present invention, and such modifications and variations are all within the scope defined by the appended claims.

Claims

1. A motion device used in a fully automatic biochip spotter, characterized in that: include: A mobile platform (2) is arranged on the working platform (1) via a first driving mechanism, and the first driving mechanism is suitable for driving the mobile platform (2) to move in the Y-axis direction with the end surface of the working platform (1) as a reference; The sample spotting component comprises a base (3), a second driving mechanism, a third driving mechanism and a sample spotting module (4), wherein the base (3) is arranged on the working platform (1) via the second driving mechanism, the second driving mechanism is suitable for driving the base (3) to move in the X-axis direction, and the sample spotting module (4) is arranged on the base (3) via the third driving mechanism, the third driving mechanism is suitable for driving the sample spotting module (4) to move in the Z-axis direction.

2. The exercise device according to claim 1, characterized in that The first driving mechanism comprises: A first guide slider (211) is arranged on the working platform (1); A first slide rail (212) is arranged at the bottom of the mobile platform (2) and cooperates with the guide groove on the first guide slider (211); A first lead screw (22) is fixedly arranged on the working platform (1); A first driving motor (23) is arranged on the mobile platform (2), and a first rotating member cooperating with the first lead screw (22) is arranged inside the first driving motor (23).

3. The exercise device according to claim 2, characterized in that: The first lead screw (22) extends along the Y-axis direction.

4. The exercise device according to claim 2, characterized in that: A first roller (241) and a second roller (242) are provided at the edge of the mobile platform (2).

5. The exercise device according to claim 2, characterized in that: A first micro switch (25) is arranged on the moving path of the moving platform (2), and the first micro switch (25) is fixedly arranged on the working platform (1).

6. The exercise device according to claim 1, characterized in that: The second driving mechanism comprises: A first bracket (31) and a second bracket (32) are respectively arranged on opposite sides of the working platform (1), and the mobile platform (2) is located between the first bracket (31) and the second bracket (32); A second slide rail (331), one end of which is connected to the first bracket (31), and the other end of which is connected to the second bracket (32); A second sliding block (332) is fixedly arranged on the base (3), the second sliding block (332) having a sliding groove that cooperates with the second sliding rail (331) for guiding; A second lead screw (34), one end of which is fixedly disposed on the first bracket (31), and the other end of which is fixedly disposed on the second bracket (32); A second drive motor (35) is arranged on the base (3), and a second rotating member cooperating with the second lead screw (34) is arranged inside the second drive motor (35).

7. The exercise device according to claim 6, characterized in that The second slide rail (331) is arranged between the first bracket (31) and the second bracket (32) and extends along the X-axis direction; the second lead screw (34) is arranged between the first bracket (31) and the second bracket (32) along the extension direction of the X-axis.

8. The exercise device according to claim 7, characterized in that The first bracket (31) is provided with a first guide groove (311), and the second bracket (32) is provided with a second guide groove (321); the first guide groove (311) and the second guide groove (321) are arranged opposite to each other in the Y-axis direction and extend in the Z-axis direction; two ends of the second slide rail (331) are respectively located in the first guide groove (311) and the second guide groove (321).

9. The exercise device according to claim 1, characterized in that: The third driving mechanism comprises: A third slide rail (51) is arranged on the base (3); A third sliding block (52) is arranged on the spotting module (4), and the third sliding block (52) has a sliding groove that cooperates with the third sliding rail; A third lead screw (53) is fixedly arranged on the base (3); The third driving motor (54) is arranged on the spotting module (4), and a third rotating member cooperating with the third lead screw (53) is arranged inside the third driving motor (54).

10. The exercise device according to claim 1, characterized in that A second micro switch (55) is provided on the base (3), and the second micro switch (55) is located on the moving path of the shell of the spotting module (4).

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