Fresh water turbellarian worm breeding device

Abstract

The utility model discloses a fresh water turbellarian worm culture device which comprises a turbellarian worm culture device and a nematode culture device placed in the turbellarian worm culture device, the nematode culture device comprises a solid culture medium, and a plurality of nematodes and / or nematode eggs are wrapped in the solid culture medium. A slow-release regulator is arranged above the solid culture medium; the turbellarian worm culture device comprises a culture tank, water is contained in the culture tank, a slow-release adjusting body can be a slicing machine or an extruding machine or water flow, the machine or the object can divide a solid culture medium and nematodes and / or eggs of the nematodes into a plurality of small parts, the small parts are slowly released into the culture tank, and fresh water turbellarian worms are cultured in the culture tank. In this way, in the slow-release regular feeding mode, the turbellarian bellarian worms can completely eat food as far as possible without remaining food as far as possible, so that it is guaranteed that water is not polluted, water quality is not reduced, frequent water changing and feed changing are avoided, and the problem that the artificial breeding cost is high is solved.

Description

Technical Field

[0001] The utility model belongs to the technical field of protozoa breeding, and particularly relates to a freshwater planarian breeding device for freshwater small planarians with the ability to prey on nematodes. Background Art

[0002] Planarians are representative animals of the phylum Platyhelminthes and are widely present in the natural environment. Their regenerative ability has important scientific research value in the status of the ecosystem. Due to the impact of environmental pollution, the number of planarians in nature has gradually decreased, and the capture difficulty has increased. It is difficult to obtain enough planarian materials for research in application directions.

[0003] Planarians are carnivorous animals and feed on living worms, small crustaceans, and insect larvae, etc. under natural conditions. In the early 20th century, scientists began to explore the artificial breeding methods of planarians. Currently, fresh pig liver, beef liver, fish spleen, or cooked egg yolk can be used for artificial feeding. However, in the above feeding methods, the feed is prone to rot and stink in the water body, seriously affecting the water quality, and the food source amount is difficult to control. Since planarians have high requirements for water quality, this method requires frequent water changes and feed replacements, resulting in high artificial breeding costs.

[0004] Therefore, those skilled in the art are committed to developing a freshwater planarian breeding device. Summary of the Invention

[0005] The purpose of the utility model is to provide a freshwater planarian breeding device to solve the problems of difficult control of the breeding quantity of planarians, easy waste, high cost, and stinking water body in the current breeding.

[0006] To solve the above problems, the utility model provides a freshwater planarian breeding device, which includes a planarian culture device and a nematode culture device placed in the planarian culture device. The nematode culture device includes a solid culture medium, and several nematodes and / or nematode eggs are wrapped in the solid culture medium. A slow-release regulator is arranged above the solid culture medium; the planarian culture device includes a culture tank filled with water.

[0007] Further, the slow-release regulator includes a water pump. The water outlet of the water pump is connected to a drip water pipe, and the drip water pipe is located directly above the solid culture medium. The water inlet of the water pump is located in the culture tank.

[0008] Further, the solid culture medium is composed of food-grade agar powder, pure water, and nutrients; the nutrients are one or a combination of corn flour, liquid pure milk, beef extract, and yeast powder.

[0009] Further, one end of an air inlet pipe is also connected in the culture tank, and the other end of the air inlet pipe is connected to an air pump communicating with the outside.

[0010] Further, the solid medium is placed in a nematode culture box. Inside the nematode culture box, a sponge water retention layer, a solid medium, and a cotton filter layer are stacked in sequence from top to bottom. A plurality of through nematode passage holes are provided at the bottom of the nematode culture box.

[0011] Further, a sieve mesh with a mesh number of 200 - 400 is also provided between the solid medium and the cotton filter layer.

[0012] Further, the mesh number of the sieve mesh is 350.

[0013] Further, the nematodes are Rhabditis nematodes or Panagrolaimus rigidus.

[0014] The beneficial effects of the present utility model are as follows: The freshwater planarian breeding device of the present utility model includes a planarian culture device and a nematode culture device placed inside the planarian culture device. The nematode culture device includes a solid medium, and a plurality of the nematodes and / or nematode eggs are wrapped inside the solid medium. A slow-release regulator is provided above the solid medium; the planarian culture device includes a culture tank filled with water. The slow-release regulator can be a slicing machine, an extruder, or a water flow. Such machines or objects can divide the solid medium and the nematodes and / or nematode eggs into multiple small portions, enabling them to be slowly released into the culture tank. Freshwater planarians are cultured in the culture tank. In this way, under this slow-release and regular feeding method, the freshwater planarians can eat as much of the food as possible when eating, with as little remaining as possible, thereby ensuring that the water body will not be polluted, reducing the water quality, and enabling better control of the water quality, avoiding frequent water changes and feed replacements, and solving the problem of high artificial breeding costs. Description of the Drawings

[0015] Figure 1 is a schematic structural diagram of the planarian culture device of the present utility model;

[0016] Figure 2 is a schematic structural diagram of the nematode culture box of the present utility model. Detailed Embodiments

[0017] In order to make the objectives, technical solutions, and advantages of the present utility model clearer, the following further elaborates on the present utility model in conjunction with embodiments. It should be understood that the specific embodiments described herein are only used to explain the present utility model and are not used to limit the present utility model.

[0018] All features disclosed in this specification, or steps in all methods or processes disclosed, except for mutually exclusive features and / or steps, can be combined in any way, and unless otherwise specifically stated, can be replaced by other equivalent or similar-purpose alternative features, that is, unless specifically stated, each feature is only one embodiment among a series of equivalent or similar features.

[0019] As Figure 1 and Figure 2 shown, a freshwater planarian breeding device includes a planarian culture device and a nematode culture device placed inside the planarian culture device. The nematode culture device includes a solid medium 6, and several nematodes and / or nematode eggs are wrapped inside the solid medium 6. A slow-release regulator is arranged above the solid medium 6; the planarian culture device includes a culture tank 2 filled with water. The slow-release regulator can be a slicer, an extruder or a water flow. Such machines or objects can divide the solid medium 6 and nematodes and / or nematode eggs into multiple small portions, enabling them to be slowly released into the culture tank 2. Freshwater planarians are cultured in the culture tank 2. In this way, under this slow-release and regular feeding method, freshwater planarians can eat as much of the food as possible without much remaining when feeding, thus ensuring that the water body will not be polluted, reducing the water quality, and enabling better control of the water quality, avoiding frequent water changes and feed replacements, and solving the problem of high artificial breeding costs.

[0020] Specifically, the nematode culture device includes a nematode culture box 12. Inside the nematode culture box 12, a sponge water-retaining layer 13, a solid medium 6 and a cotton filtration layer 14 are stacked in sequence from top to bottom. A number of penetrating nematode passage holes 16 are arranged at the bottom of the nematode culture box 12. The main material of the sponge water-retaining layer is water-absorbing sponge, which is used for water control; the solid medium is used for nematode culture; the main material of the cotton filtration layer is cotton, which is used for filtering other impurities except nematodes; a sieve mesh 15 with a mesh number of 200 - 400 is also arranged between the solid medium 6 and the cotton filtration layer 14. In this embodiment, the mesh number of the sieve mesh 15 is 350, which is used to separate the nematode solid medium and the cotton adsorption layer.

[0021] The planarian culture device 1 includes a culture tank 2, a culture medium support 3, and a drip water pipe 4. Water 5 is poured into the culture tank 2. The culture medium support 3 is installed in the culture tank 2 and is above the water surface. The nematode culture box 12 containing the solid culture medium 6 with nematodes is placed on the top of the culture medium support 3. The outlet of the drip water pipe 4 is directly above the solid culture medium 6, and the inlet is externally connected to a water source. In this embodiment, the inlet of the drip water pipe 4 is connected to the outlet of a water pump 7, and the water pump 7 is placed in the culture tank 2 and immersed in the water. A planarian escape-proof net 8 is also installed between the water pump 7 and the culture medium support 3 in the culture tank 2. An air inlet pipe 9 is also provided in the culture tank 2. The inlet of the air inlet pipe 9 is connected to an air pump 10, and the outlet is connected to an air pump 11. Add the precipitated tap water into the culture tank 2, keep the water level height at 6 to 7 cm, turn on the water pump 7, slowly release the nematodes in the solid culture medium, inoculate the planarians into the culture tank, and turn on the air pump to keep the dissolved oxygen in the water body above 10 mg / L.

[0022] In this embodiment, the slow-release regulator can be a slicing machine, an extruder, or a water flow. Such machines can divide the solid culture medium 6 and nematodes into multiple small portions and slowly release them into the culture tank 2. However, it is preferably to use a water flow, that is, the size of the water flow is controlled by a water pump or a valve. The water flow flushes or drips on the solid culture medium 6, slowly dissolving the solid culture medium 6 and nematodes into the culture tank 2. The cost is lower than that of a slicing machine or an extruder, and the water flow circulation can further remove impurities in the culture tank and increase the dissolved oxygen in the water body.

[0023] In this implementation scheme, the water in the aquaculture tank is dripped onto the sponge water retention layer of the nematode culture box through a drip water pipe by a water pump. Due to the water absorption saturation characteristic of the sponge, when continuously dripping water onto the sponge, the sponge gradually absorbs water to saturation, and the excess water will penetrate downward to the nematode solid medium. Since the solid medium has no water retention ability, the water that penetrates downward will carry the nematodes and viscous impurities (mainly starch, protein particles, and dead nematodes) in the solid medium and slowly pass through a 350-mesh sieve and enter the cotton adsorption layer. Most of the impurities and dead nematodes will be adhered to the cotton, while most of the live nematodes will drip into the aquaculture tank along with the water flow, realizing the automatic feeding of planarian nematode bait. And after passing through the cotton adsorption layer and then entering the aquaculture tank, it solves the problem of water pollution in the aquaculture tank caused by excessive impurities. At the same time, since there are a large number of nematodes and egg masses in the culture medium during the cultivation and reproduction of nematodes, the slow water flow cannot completely release the nematodes in the solid medium, but only releases a small amount of nematodes. Most of the nematodes and egg masses still remain in the solid medium. The sponge water retention layer above it can keep the moisture of the solid medium suitable for the survival of nematodes, greatly extending the service time of the nematode solid medium, realizing the three-level ecological aquaculture of solid medium, nematodes, and planarians, greatly reducing the feeding frequency, and thus reducing the labor cost. A 350-mesh planarian escape-proof net is designed in the aquaculture tank, which can not only prevent the planarians in the aquaculture tank from overflowing and entering the water pump, causing the death of planarians, but also pump the water in the aquaculture tank into the drip water pipe and enter the nematode release cycle to form circulating water, ensuring the stable water level in the aquaculture tank. The pumped water increases the water evaporation rate. During the aquaculture process, the water level of the aquaculture tank can be stabilized by fixing the water level controller of the aquaculture tank. The function of the air pump is to keep the dissolved oxygen content in the water body of the aquaculture tank not less than 10 mg / L, and for the environmental temperature control, normal operation is sufficient, and the environmental temperature is controlled at 28 to 30 °C.

[0024] The preferred specific embodiments of the present invention have been described in detail above. It should be understood that those of ordinary skill in the art can make many modifications and variations based on the concept of the present invention without creative work. Therefore, all technical solutions that can be obtained by those skilled in the art in the technical field of the present invention based on the concept of the present invention through logical analysis, reasoning, or limited experiments on the basis of the prior art should be within the protection scope determined by the claims.

Claims

1. A freshwater turbellarian breeding device, characterized in that: The invention comprises a planarian culture device and a nematode culture device placed in the planarian culture device, wherein the nematode culture device comprises a solid culture medium (6), wherein a plurality of nematodes and / or nematode eggs are encapsulated in the solid culture medium (6), and a slow-release regulating body is arranged above the solid culture medium (6); the planarian culture device comprises a breeding tank (2), wherein the breeding tank (2) is filled with water.

2. The freshwater turbellarian breeding device according to claim 1, characterized in that: The slow-release regulator comprises a water pump (7), the water outlet of the water pump (7) is connected to a dripping pipe (4), the dripping pipe (4) is located directly above the solid culture medium (6), and the water inlet of the water pump (7) is located in the breeding tank (2).

3. The freshwater turbellarian breeding device according to claim 1, characterized in that: One end of an air intake pipe (9) is also connected to the breeding tank (2), and the other end of the air intake pipe (9) is connected to an air pump (10) that is in communication with the outside.

4. The freshwater turbellarian breeding device according to claim 1, characterized in that: The solid culture medium (6) is placed in a nematode culture box (12), wherein a sponge water-retaining layer (13), a solid culture medium (6) and a cotton filter layer (14) are sequentially stacked from top to bottom in the nematode culture box (12), and a plurality of nematode passing holes (16) are provided at the bottom of the nematode culture box (12).

5. The freshwater turbellarian breeding device according to claim 4, characterized in that: A screen (15) with a mesh size of 200 to 400 is also provided between the solid culture medium (6) and the cotton filter layer (14).

6. The freshwater turbellarian culture device according to claim 5, characterized in that: The mesh number of the sieve (15) is 350 meshes.

7. The freshwater turbellarian breeding device according to any one of claims 1 to 6, characterized in that: The nematode is a Rhabditis elegans or a Holodontia resurrection nematode.

Images