Subpackaging and freezing storage device for serum specimens

Abstract

The utility model discloses a subpackage freezing storage device for serum specimens, which relates to the technical field of medical equipment and comprises a subpackage tube, a subpackage seat and a thawing extrusion seat, the subpackage tube is of a square column-shaped structure with square ribs fixedly connected to the centers of two sides, a round through hole is formed in the center of the subpackage tube, and the round through hole is communicated with the subpackage seat. The bottom of a round through hole of the split charging pipe is fixedly connected with a split charging needle-threading rubber plug, the split charging pipe can be used for split charging and storing blood samples, the split charging seat can be matched with the split charging pipe through the split charging seat, uniform split charging of the serum samples is achieved, whole freezing storage is achieved, only the split charging seat needs to be labeled, distinguishing and finding are convenient, and the split charging efficiency is improved. By means of the arranged thawing extrusion base, the thawing extrusion base can be matched with the split charging pipe, thawing of serum can be accelerated, transfer and detection of the serum can be achieved without transferring the serum to a PCR pipe, multiple sets of operation can be conducted at the same time, and the operation steps of serum sample detection are greatly reduced.

Description

Technical Field

[0001] The utility model relates to the technical field of medical devices, in particular to a sub-packaging freezing storage device for serum specimens. Background Technique

[0002] Human serum specimens are often at the level of 1-2 ml. Before enzyme-linked immunosorbent assay (ELISA) tests a large number of serum samples in scientific research, each serum sample often needs to be quantitatively sub-packaged and frozen for storage. Each serum specimen is sub-packaged into 3-5 tubes, and the sub-packaging volume of each tube is 50-150 μl, and then stored frozen at -20°C or -80°C. The numbering of such sub-packaged serum specimens is cumbersome and the large quantity is not easy to store. When adding samples during ELISA tests, it is necessary to check and add samples to the wells of the enzyme-linked immunosorbent assay (ELISA) plate one by one. During batch testing, it leads to a long sample addition time, large differences in results, easy to cause poor repeatability, and affects the results. Therefore, we propose a sub-packaging freezing storage device for serum specimens. Content of the Utility Model

[0003] The purpose of the utility model is to solve the problems that human serum specimens are often at the level of 1-2 ml. Before enzyme-linked immunosorbent assay (ELISA) tests a large number of serum samples in scientific research, each serum sample often needs to be quantitatively sub-packaged and frozen for storage. Each serum specimen is sub-packaged into 3-5 tubes, and the sub-packaging volume of each tube is 50-150 μl, and then stored frozen at -20°C or -80°C. The numbering of such sub-packaged serum specimens is cumbersome and the large quantity is not easy to store. When adding samples during ELISA tests, it is necessary to check and add samples to the wells of the enzyme-linked immunosorbent assay (ELISA) plate one by one. During batch testing, it leads to a long sample addition time, large differences in results, easy to cause poor repeatability, and affects the results. The utility model provides a sub-packaging freezing storage device for serum specimens.

[0004] The utility model specifically adopts the following technical solutions to achieve the above purpose:

[0005] A sub-packaging freezing storage device for serum specimens, including a sub-packaging tube, a sub-packaging base, and a thawing and extrusion base. The sub-packaging tube is a square columnar structure with square convex edges fixedly connected to the centers of both sides, and a circular through hole is opened in the center of the sub-packaging tube. A sub-packaging needle rubber plug is fixedly connected to the bottom of the circular through hole of the sub-packaging tube, and a sub-packaging moving piston is slidably connected to the upper end of the circular through hole of the sub-packaging tube. The sub-packaging base is a regular polygonal prism structure, and slots matching the outer shape of the sub-packaging tube are opened at the centers of the upper edges corresponding to each side wall of the sub-packaging base, and a liquid distribution mechanism is arranged in the center of the sub-packaging base. Eight slots matching the outer shape of the sub-packaging tube are opened at the front side of the upper end of the thawing and extrusion base corresponding to the pitch of the wells of the enzyme-linked immunosorbent assay (ELISA) plate, and an extrusion mechanism is arranged at the upper end of the thawing and extrusion base.

[0006] Further, the liquid separation mechanism includes a dispensing tube with a dispensing liquid suction cavity opened at the center of the upper end. A pressing piston is movably inserted into the center of the dispensing liquid suction cavity, and a dispensing push rod is fixedly connected to the upper end of the pressing piston. The center of the bottom of the slot opened at the upper edge of the dispensing seat is fixedly connected with a dispensing needle, and the bottoms of the six dispensing needles are all communicated with the bottom of the inner cavity of the dispensing liquid suction cavity through a dispensing communication tube buried at the bottom of the dispensing tube.

[0007] Further, the extrusion mechanism includes an extrusion pressing plate which is slidably installed on the upper side of the thawing extrusion seat through two side guiding and moving units. The bottom of the extrusion pressing plate is fixedly connected with extrusion push rods corresponding to the centers of the upper ends of a plurality of dispensing tubes. A disposable liquid separation head is inserted into the bottom of the slot on the side wall of the thawing extrusion seat, and a disposable liquid separation needle is fixedly connected to the upper end of the disposable liquid separation head. The disposable liquid separation needle is communicated with the inner cavity of the disposable liquid separation head.

[0008] Further, the guiding and moving unit includes a guiding sliding seat and a guiding insertion rod. Two side guiding insertion rods are fixedly connected to the bottom of the extrusion pressing plate. The guiding insertion rods penetrate through the guiding sliding seat and are movably inserted, and anti-loosening nuts are threadedly connected to the bottoms of the guiding insertion rods. A jacking spring is sleeved on the outer wall of the guiding insertion rod corresponding to the upper section of the guiding sliding seat, and the upper end of the jacking spring abuts against the bottom surface of the extrusion pressing plate.

[0009] Further, a circular convex strip is fixedly connected to the upper edge of the disposable liquid separation head, and a circular step is opened at the upper edge of the insertion hole corresponding to the disposable liquid separation head at the bottom of the slot on the side wall of the thawing extrusion seat.

[0010] Further, an operation handle is fixedly connected to the upper end of the back side of the thawing extrusion seat.

[0011] Further, a plugging convex block is fixedly connected to the upper end of the outer side of the dispensing tube.

[0012] The beneficial effects of the present utility model are as follows:

[0013] 1. Through the provided dispensing tube, the dispensing tube can dispense and store blood samples. Through the provided dispensing seat, the dispensing seat can cooperate with the dispensing tube to achieve uniform dispensing of serum samples and overall frozen storage. Only by labeling the dispensing seat, it is convenient to distinguish and find. Through the provided thawing extrusion seat, the thawing extrusion seat can cooperate with the dispensing tube to accelerate the thawing of serum, and the transfer and detection of serum can be realized without transferring to a PCR tube. At the same time, multiple groups of operations can be carried out, greatly reducing the operation steps of serum sample detection. At the same time, eight samples are dropped, greatly improving the detection efficiency of serum samples. The sample addition time is short during batch detection, the result difference is small, and it is not easy to cause poor repeatability, ensuring the accuracy of the detection result.

[0014] 2. The utility model realizes the automatic sub-packaging of sucking and squeezing out serum samples through the arranged sub-packaging liquid suction cavity. The extrusion piston in the sub-packaging liquid suction cavity can be pulled up and down by the sub-packaging push rod, which is simple to operate and convenient to use. Through the arranged extrusion pressing plate, the extrusion pressing plate can be limited by the guiding and moving unit and move up and down along the upper end of the thawing and extrusion seat. The extrusion push rod is used to push the sub-packaging moving piston to move, and squeeze out the serum sample in the sub-packaging tube, which is simple, practical and convenient to operate.

[0015] 3. The utility model is provided with a guiding sliding seat and a guiding insertion rod. The guiding insertion rod penetrates through the guiding sliding seat and is movably inserted, so as to limit and guide the up and down movement of the extrusion pressing plate, ensuring the stable movement of the extrusion pressing plate. Through the arranged jacking spring, the jacking spring keeps the extrusion pressing plate moving upward without external force, so that the extrusion pressing plate keeps the extrusion push rod separated from the sub-packaging moving piston when there is no external force, avoiding accidental contact with the sub-packaging moving piston. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 is a three-dimensional view of the cooperation between the sub-packaging seat and the sub-packaging tube during the sub-packaging and freezing storage of the utility model;

[0017] Figure 2 is a side sectional view of the cooperation between the sub-packaging seat and the sub-packaging tube during the sub-packaging and freezing storage of the utility model;

[0018] Figure 3 is a three-dimensional view of the cooperation between the sub-packaging tube and the thawing and extrusion seat during the detection of the utility model;

[0019] Figure 4 is a side sectional view of the cooperation between the sub-packaging tube and the thawing and extrusion seat during the detection of the utility model.

[0020] Reference numerals: 1. Sub-packaging tube; 2. Sub-packaging seat; 3. Thawing and extrusion seat; 4. Sub-packaging needle-piercing rubber stopper; 5. Sub-packaging moving piston; 6. Sub-packaging liquid suction cavity; 7. Extrusion piston; 8. Sub-packaging push rod; 9. Sub-packaging connecting pipe; 10. Sub-packaging needle; 11. Disposable liquid separating head; 12. Disposable liquid separating needle; 13. Extrusion pressing plate; 14. Extrusion push rod; 15. Guiding sliding seat; 16. Guiding insertion rod; 17. Jacking spring; 18. Operation grip; 19. Plugging and unplugging convex block. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0021] For the purpose of making the objectives, technical solutions and advantages of the embodiments of the utility model clearer, the technical solutions in the embodiments of the utility model will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the utility model.

[0022] Please refer to Figure 1 - Figure 4, the present utility model provides a sub-packaging freezing storage device for serum specimens, including a sub-packaging tube 1, a sub-packaging base 2 and a thawing and extrusion base 3. The sub-packaging tube 1 is a square columnar structure with square convex ribs fixedly connected to the centers on both sides, and a circular through-hole is opened in the center of the sub-packaging tube 1. A sub-packaging needle rubber stopper 4 is fixedly connected to the bottom of the circular through-hole of the sub-packaging tube 1, and a sub-packaging moving piston 5 is slidably connected to the upper end of the circular through-hole of the sub-packaging tube 1. The sub-packaging base 2 is a regular polygonal prism structure, and slots matching the outer shape of the sub-packaging tube 1 are opened at the centers corresponding to the edges of the upper end of the sub-packaging base 2 for each side wall, and a liquid distribution mechanism is arranged at the center of the sub-packaging base 2. At the front side of the upper end of the thawing and extrusion base 3, eight slots matching the outer shape of the sub-packaging tube 1 are opened corresponding to the spacing of the enzyme-labeled plate holes, and an extrusion mechanism is arranged at the upper end of the thawing and extrusion base 3.

[0023] In this embodiment, preferably, the liquid distribution mechanism includes a sub-packaging liquid suction cavity 6 opened at the center of the upper end of the sub-packaging tube 1. A squeezing piston 7 is movably inserted into the center of the sub-packaging liquid suction cavity 6, and a sub-packaging push rod 8 is fixedly connected to the upper end of the squeezing piston 7. A sub-packaging needle 10 is fixedly connected to the center of the bottom of the slot opened at the edge of the upper end of the sub-packaging base 2, and the bottoms of the six sub-packaging needles 10 are all communicated with the bottom of the inner cavity of the sub-packaging liquid suction cavity 6 through a sub-packaging communication pipe 9 buried at the bottom of the sub-packaging tube 1; through the arranged sub-packaging liquid suction cavity 6, the squeezing piston 7 in the sub-packaging liquid suction cavity 6 can be pulled up and down by the sub-packaging push rod 8 to realize sucking and squeezing out the serum sample, automatic sub-packaging, simple operation and convenient use.

[0024] In this embodiment, preferably, the extrusion mechanism includes an extrusion pressing plate 13. The extrusion pressing plate 13 is slidably installed on the upper side of the thawing and extrusion base 3 through two side guiding and moving units, and an extrusion push rod 14 is fixedly connected to the bottom of the extrusion pressing plate 13 corresponding to the center positions of the upper ends of a plurality of sub-packaging tubes 1. A disposable liquid distribution head 11 is inserted into the bottom of the slot on the side wall of the thawing and extrusion base 3, and a disposable liquid distribution needle 12 is fixedly connected to the upper end of the disposable liquid distribution head 11. The disposable liquid distribution needle 12 is communicated with the inner cavity of the disposable liquid distribution head 11; through the arranged extrusion pressing plate 13, the extrusion pressing plate 13 can be limited by the guiding and moving unit and move up and down along the upper end of the thawing and extrusion base 3, and the sub-packaging moving piston 5 is pushed by the extrusion push rod 14 to extrude the serum sample in the sub-packaging tube 1, with simple operation and convenient use.

[0025] The guide rod 16 is inserted through the guide slide 15 and is movably inserted into the guide slide 15, and the bottom of the guide rod 16 is threadedly connected with an anti-slip nut, and the outer wall of the guide rod 16 is sleeved with a lifting spring 17 corresponding to the upper end of the guide slide 15, and the upper end of the lifting spring 17 is against the bottom surface of the extrusion press plate 13; through the set guide slide 15 and guide rod 16, the guide rod 16 penetrates the guide slide 15 and is movably inserted, and the up and down movement of the extrusion press plate 13 is limited and guided to ensure the stable movement of the extrusion press plate 13, and through the set lifting spring 17, the lifting spring 17 keeps the extrusion press plate 13 from moving upward when not affected by external force, so that the extrusion press plate 13 keeps the extrusion push rod 14 separated from the packaging moving piston 5 when not affected by external force, avoiding accidental touching of the packaging moving piston 5.

[0026] In the present embodiment, preferably, a circle of annular convex strips are fixedly connected to the upper edge of the disposable dispensing head 11, and an annular step is provided at the bottom of the side wall slot of the thawing extrusion seat 3 corresponding to the upper edge of the insertion hole of the disposable dispensing head 11; the annular convex strips are provided to facilitate the snap-fitting between the disposable dispensing head 11 and the bottom of the side wall slot of the thawing extrusion seat 3.

[0027] In the present embodiment, preferably, an operating handle 18 is fixedly connected to the upper end of the back side of the thawing extrusion seat 3; through the provided operating handle 18, the operating handle 18 can be held by an operator.

[0028] In this embodiment, preferably, an upper end of the dispensing tube 1 facing outward is fixedly connected with an extraction and extraction protrusion 19; the extraction and extraction protrusion 19 is provided to facilitate the insertion and extraction of the dispensing tube 1.

[0029] Working principle and usage process of the utility model: When sub-packaging and freezing serum samples, according to the requirement of the number of sub-packagings, select the sub-packaging base 2 with the corresponding number of side walls, insert the sub-packaging tubes 1 with the number of side walls corresponding to the sub-packaging base 2 into the side wall slots of the sub-packaging base 2, so that the sub-packaging needles 10 on the side walls of the sub-packaging base 2 are communicated with the inner cavities of the sub-packaging tubes 1. A rubber injection plug is fixedly connected to the bottom of the sub-packaging base 2 corresponding to the center of the sub-packaging liquid suction cavity 6. Connect the serum sample to the bottom of the sub-packaging liquid suction cavity 6 through an injection needle. Hold the sub-packaging push rod 8 and pull up and squeeze the piston 7 to suck the serum sample into the sub-packaging liquid suction cavity 6. Then pull out the injection needle, press the sub-packaging push rod 8, and the sub-packaging push rod 8 pushes the squeezing piston 7 to disperse and squeeze the serum sample at the bottom of the sub-packaging liquid suction cavity 6 into the multiple sub-packaging tubes 1 on the side walls through the multiple sub-packaging connecting tubes 9. The serum liquid pushes the sub-packaging moving piston 5 to move up and store in the sub-packaging tube 1. Then put the whole sub-packaging base 2 into the freezing equipment for freezing storage. When detecting the serum sample, place a disposable liquid separating head 11 at the bottom of the side wall slot of the thawing and extrusion base 3. Then pull out one sub-packaging tube 1 from each sub-packaging base 2 that needs to be sampled and insert it into the side wall slot of the thawing and extrusion base 3. The thawing and extrusion base 3 is used to assist in thawing the serum sample in the sub-packaging tube 1. After thawing, press the extrusion pressing plate 13, and the extrusion push rod 14 pushes the sub-packaging moving piston 5 to squeeze the serum sample in the sub-packaging tube 1, so that the serum sample is squeezed into the disposable liquid separating head 11 through the disposable liquid separating needle 12 and dripped into the wells of the microplate through the bottom of the disposable liquid separating head 11 for detection. Through the provided sub-packaging tube 1, the sub-packaging tube 1 can sub-package and store blood samples. Through the provided sub-packaging base 2, the sub-packaging base 2 can cooperate with the sub-packaging tube 1 to realize uniform sub-packaging of the serum sample and overall freezing storage. Only label the sub-packaging base 2 for easy identification and search. Through the provided thawing and extrusion base 3, the thawing and extrusion base 3 can cooperate with the sub-packaging tube 1, accelerate the thawing of the serum, and realize the transfer and detection of the serum without transferring it to a PCR tube. At the same time, multiple groups of operations can be carried out, greatly reducing the operation steps of serum sample detection. At the same time, eight samples are dripped, greatly improving the detection efficiency of serum samples. When batch detecting, the sample adding time is short, the result difference is small, it is not easy to cause poor repeatability, and the accuracy of the detection result is ensured.

[0030] The above description of the disclosed embodiments enables those skilled in the art to implement or use the utility model. Various modifications to these embodiments will be obvious to those skilled in the art. The general principles defined herein can be implemented in other embodiments without departing from the spirit or scope of the utility model. Therefore, the utility model will not be limited to the embodiments shown herein, but rather to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A serum specimen packaging and freezing storage device, characterized in that: The invention comprises a dispensing tube (1), a dispensing seat (2) and a thawing extrusion seat (3); the dispensing tube (1) is a square columnar structure with square convex edges fixedly connected to the centers of both sides, and a circular through hole is provided in the center of the dispensing tube (1); a dispensing needle rubber plug (4) is fixedly connected to the bottom of the circular through hole of the dispensing tube (1), and a dispensing movable piston (5) is slidably connected to the upper end of the circular through hole of the dispensing tube (1); the dispensing seat (2) is a regular polygonal columnar structure, and a slot matching the shape of the dispensing tube (1) is provided at the center of each side wall of the upper edge of the dispensing seat (2), and a liquid separation mechanism is provided in the center of the dispensing seat (2); eight slots matching the shape of the dispensing tube (1) are provided on the front side of the upper end of the thawing extrusion seat (3) corresponding to the spacing of the holes of the enzyme labeling plate, and an extrusion mechanism is provided at the upper end of the thawing extrusion seat (3).

2. A serum specimen subpackaging and freezing storage device according to claim 1, characterized in that: The liquid dispensing mechanism comprises a dispensing liquid suction chamber (6) provided at the center of the upper end of a dispensing tube (1), an extrusion piston (7) movably inserted at the center of the dispensing liquid suction chamber (6), and a dispensing push rod (8) fixedly connected to the upper end of the extrusion piston (7), a dispensing needle (10) fixedly connected to the bottom center of a slot provided at the upper end edge of the dispensing seat (2), and the bottoms of the six dispensing needles (10) are connected to the bottom of the inner cavity of the dispensing liquid suction chamber (6) through a dispensing connecting pipe (9) buried in the bottom of the dispensing tube (1).

3. The serum specimen subpackaging and freezing storage device according to claim 1, characterized in that: The extrusion mechanism comprises an extrusion pressing plate (13), the extrusion pressing plate (13) is slidably mounted on the upper side of the thawing extrusion seat (3) via guide movable units on both sides, and an extrusion push rod (14) is fixedly connected to the center position of the upper ends of a plurality of dispensing tubes (1) at the bottom of the extrusion pressing plate (13), a disposable liquid dispensing head (11) is inserted into the bottom of a slot on the side wall of the thawing extrusion seat (3), and a disposable liquid dispensing needle (12) is fixedly connected to the upper end of the disposable liquid dispensing head (11), and the disposable liquid dispensing needle (12) is communicated with the inner cavity of the disposable liquid dispensing head (11).

4. The serum specimen subpackaging and freezing storage device according to claim 3, characterized in that: The guide movable unit comprises a guide slide (15) and a guide rod (16), both sides of the bottom of the extrusion pressing plate (13) are fixedly connected with the guide rods (16), and the guide rods (16) penetrate the guide slide (15) and are movably plugged in, and the bottom of the guide rod (16) is threadedly connected with an anti-slip nut, and the outer wall of the guide rod (16) corresponds to a section of the upper end of the guide slide (15) and is sleeved with a lifting spring (17), and the upper end of the lifting spring (17) is against the bottom surface of the extrusion pressing plate (13).

5. The serum specimen subpackaging and freezing storage device according to claim 4, characterized in that: The upper edge of the disposable liquid dispensing head (11) is fixedly connected with a circle of annular convex strips, and an annular step is provided at the bottom of the side wall slot of the thawing extrusion seat (3) corresponding to the upper edge of the insertion hole of the disposable liquid dispensing head (11).

6. The serum specimen subpackaging and freezing storage device according to claim 1, characterized in that: An operating handle (18) is fixedly connected to the upper end of the back side of the thawing extrusion seat (3).

7. The serum specimen subpackaging and freezing storage device according to claim 1, characterized in that: The upper end of the dispensing tube (1) facing outward is fixedly connected with a plug-in / pull-out protrusion (19).

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