SPR sensor and SPR detection equipment

Abstract

The utility model discloses an SPR (Surface Plasmon Resonance) sensor and SPR detection equipment, the SPR sensor comprises a base, a reaction device is arranged at the bottom of the base, a prism is arranged at the top of the base, an incident light source device is arranged on one side of the prism, the incident light source device comprises a laser, a reflection receiving device is arranged on the other side of the prism, and the laser is connected with the reflection receiving device. The reflecting and receiving device comprises a receiver, preferably, a polaroid and a collecting lens are symmetrically arranged on the two sides of the prism, optical information and imaging quality in the SPR detection technology are effectively improved, and the problem that at the present stage, molecules are combined with media such as protein on the surface of a gold film in a natural circulation mode, so that the detection efficiency is greatly improved is solved. However, the mode cannot further promote the promotion of the combination between the two, so that the combination is insufficient.

Description

Technical Field

[0001] The utility model mainly relates to the technical field of copper melting furnaces, in particular to an SPR sensor and an SPR detection device. Background Art

[0002] Surface Plasmon Resonance (SPR) technology is a new analytical technology developed based on optical principles. It refers to the resonance phenomenon that may occur when light is totally reflected on the surface of a prism or metal film, forming an evanescent wave that enters a light-scarce medium, and there are certain plasma waves in the medium (assuming it is a precious metal) based on the premise of energy conservation when the two wavebands meet. This technology can detect the interaction between antigens and antibodies, DNA and proteins, and DNA and DNA, and has been widely used in life sciences, medical diagnosis, food safety, environmental testing and other fields.

[0003] SPR is a traditional optical phenomenon that uses light to generate evanescent waves in different media and then resonate with plasma waves, thereby constructing a biosensing analysis technology for biomolecular interactions to detect the interaction between ligands and analytes on biosensor chips.

[0004] However, the current SPR detection technology cannot achieve good focusing under the effect of light conduction, and may be affected by the external environment, resulting in light dispersion, and the focus of light cannot be adjusted, resulting in optical information and there is still room for further improvement in imaging quality.

[0005] And at the current stage, the binding of molecules to media such as proteins on the surface of the gold film is through natural flow, but this method cannot further promote the binding between the two, resulting in insufficient binding.

[0006] Therefore, an SPR sensor and an SPR detection device are needed to solve the above problems. Utility Model Content

[0007] The technical solution of the utility model aims at the technical problem that the existing technical solution is too single, provides a solution that is significantly different from the existing technology, and mainly provides an SPR sensor and an SPR detection device to solve the technical problems raised in the above background technology.

[0008] The utility model solves the above technical problems by adopting the following technical solution: an SPR sensor and an SPR detection device, comprising a base, a reaction device is arranged at the bottom of the base, a prism is arranged at the top of the base, an incident light source device is arranged on one side of the prism, the incident light source device comprises a laser, and a reflection receiving device is arranged on the other side of the prism, the reflection receiving device comprises a receiver.

[0009] Preferably, polarizers and condenser lenses are symmetrically arranged on both sides of the prism.

[0010] Preferably, a convex lens is arranged on one side of the condenser lens located at the position of the incident light source device.

[0011] Preferably, a protective light-shielding cover is arranged outside the prism. A perforation is formed at the top of the protective light-shielding cover, and an LED light source is installed in the perforation.

[0012] Preferably, one side of the protective light-shielding cover communicates with one end of a third light-shielding protection tube, and the other end of the third light-shielding protection tube communicates with a receiver.

[0013] Preferably, the other side of the protective light-shielding cover communicates with one end of a second light-shielding protection tube. The other end of the second light-shielding protection tube is slidably connected to one end of an adjustable light-shielding protection tube. The other end of the adjustable light-shielding protection tube is slidably connected to one end of a first light-shielding protection tube. The other end of the first light-shielding protection tube communicates with a laser. The inner circle of the adjustable light-shielding protection tube is fixedly connected to the outer circle of the convex lens.

[0014] Preferably, the reaction device includes a gold film. A prism is arranged at the upper end of the gold film. The gold film is installed in a card slot formed in the middle position of a base. An inlet channel plate is arranged at the lower end of the gold film. The inlet channel plate is slidably connected to an adjustment plate. One side of the adjustment plate is an inclined surface, and the other side of the adjustment plate is a hand-pushing end.

[0015] Preferably, the SPR detection device includes the above-mentioned SPR sensor.

[0016] Compared with the prior art, the beneficial effects of the present utility model are as follows:

[0017] By sliding the adjustable light-shielding protection tube, the convex lens can be driven to move, so that the focusing degree of light can be adjusted, thereby improving the optical information and the imaging quality, which is more conducive to the research of researchers.

[0018] The protective light-shielding cover can prevent the LED light source from being scattered by external factors, and the LED light source can further comprehensively receive the surface plasmon resonance (SPR) angle evanescent wave on the gold film, so as to avoid the evanescent wave caused by a single laser point source.

[0019] By manually pushing the adjustment plate to move, the molecules flowing in the inlet channel plate can be caused to move, so as to facilitate improving the binding condition of the molecules and the proteins on the gold film, and clamping parameters such as the binding, dissociation and affinity between the molecules.

[0020] The present invention will be described in detail below in conjunction with the drawings and specific embodiments. Description of the Drawings

[0021] Figure 1 is a three-dimensional schematic diagram of the present utility model;

[0022] Figure 2 is a schematic diagram of the light conduction structure of the present invention;

[0023] Figure 3 is a schematic diagram of the side view structure of the present utility model;

[0024] Figure 4 is a schematic diagram of the structure of the reaction device in the present utility model;

[0025] Figure 5 is a schematic diagram of the molecular flow in the present invention.

[0026] Reference numerals in the figures: 1 - base, 2 - reaction device, 21 - gold film, 22 - inlet channel plate, 23 - adjusting plate, 3 - protective light-shielding cover, 31 - prism, 4 - LED light source 4, 5 - incident light source device, 51 - laser, 52 - first light-shielding protection tube, 53 - adjusting light-shielding protection tube, 54 - second light-shielding protection tube, 55 - convex lens, 56 - polarizer, 57 - condenser lens, 6 - reflection receiving device, 61 - receiver, 62 - third light-shielding protection tube. Specific embodiments

[0027] For ease of understanding of the present utility model, the present utility model will be described more comprehensively below with reference to the relevant drawings. Several embodiments of the present utility model are given in the drawings, but the present utility model can be implemented in different forms and is not limited to the embodiments described in the text. On the contrary, these embodiments are provided to make the disclosure of the present utility model more thorough and comprehensive.

[0028] It should be noted that when an element is referred to as being "fixedly provided on" another element, it can be directly on the other element or there can be an intermediate element. When an element is considered to be "connected to" another element, it can be directly connected to the other element or there may be an intermediate element at the same time. The terms "vertical", "horizontal", "left", "right" and similar expressions used herein are for illustrative purposes only.

[0029] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those of ordinary skill in the technical field to which the present utility model belongs. The terms used in the specification of the present utility model herein are only for the purpose of describing specific embodiments and are not intended to limit the present utility model. The term "and / or" used herein includes any and all combinations of one or more of the related listed items.

[0030] Please refer to Figures 1-3, an SPR sensor and an SPR detection device, including a base 1, a reaction device 2 is arranged at the bottom of the base 1, a prism 31 is arranged at the top of the base 1, an incident light source device 5 is arranged on one side of the prism 31, the incident light source device 5 includes a laser 51, and a reflection receiving device 6 is arranged on the other side of the prism 31, and the reflection receiving device 6 includes a receiver 61.

[0031] Polarizers 56 and condenser lenses 57 are symmetrically arranged on both sides of the prism 31. A wire grid structure with nanoscale dimensions is etched on the glass substrate of the polarizer 56. When natural light passes through the wire grid polarizer, only the electromagnetic waves with the vibration direction parallel to the wire grid direction can pass through, while the electromagnetic waves perpendicular to the wire grid direction will be filtered out. Thus, the light after the wire grid polarizer becomes polarized light, and further polarized light can obtain more optical information, improve the imaging quality, and the condenser lens 57 can prevent the divergence of light, thereby improving the directivity effect.

[0032] A convex lens 55 is arranged on one side of the condenser lens 57 at the position of the incident light source device 5. Through the convex lens 55 and the adjustment of the convex lens 55, the size of the light point source can be further converged, so that the light point source is more focused, which is convenient for the receiver 61 to collect.

[0033] A protective light-shielding cover 3 is arranged outside the prism 31. A perforation is opened at the top of the protective light-shielding cover 3, and an LED light source 4 is installed in the perforation. Through the protective light-shielding cover 3, the LED light source 4 can be prevented from being scattered by external factors, and the LED light source 4 can further comprehensively receive the SPR angle evanescent wave on the gold film 21, so as to avoid the formation of evanescent waves caused by a single laser 51 point source.

[0034] One side of the protective light-shielding cover 3 is communicated with one end of a third light-shielding protection tube 62, and the other end of the third light-shielding protection tube 62 is communicated with the receiver 61. The other side of the protective light-shielding cover 3 is communicated with one end of a second light-shielding protection tube 54, the other end of the second light-shielding protection tube 54 is slidably connected with one end of an adjustable light-shielding protection tube 53, the other end of the adjustable light-shielding protection tube 53 is slidably connected with one end of a first light-shielding protection tube 52, the other end of the first light-shielding protection tube 52 is communicated with the laser 51, and the inner ring of the adjustable light-shielding protection tube 53 is fixedly connected with the outer ring of the convex lens 55. By sliding the adjustable light-shielding protection tube 53, the convex lens 55 can be driven to move, so as to adjust the light focusing degree, improve the optical information and the imaging quality, and be more conducive to researchers' research.

[0035] The reaction device 2 includes a gold film 21, a prism 31 is arranged at the upper end of the gold film 21, the gold film 21 is installed in a card slot opened at the middle position of the base 1, an inlet channel plate 22 is arranged at the lower end of the gold film 21, the inlet channel plate 22 is slidably connected with an adjusting plate 23, one side of the adjusting plate 23 is an inclined surface, the other side of the adjusting plate 23 is a hand-pushing end, by manually pushing the adjusting plate 23 to move, the molecules flowing in the inlet channel plate 22 can be caused to move, so as to facilitate improving the binding condition of the molecules with the protein on the gold film 21, thereby clamping parameters such as the binding, dissociation and affinity between molecules.

[0036] The present invention also provides an SPR detection device, including the above SPR sensor.

[0037] The above has made an exemplary description of the present invention in conjunction with the accompanying drawings. Obviously, the specific implementation of the present invention is not limited by the above methods. As long as such non-substantial improvements are made by adopting the method concept and technical solution of the present invention, or the concept and technical solution of the present invention are directly applied to other occasions without improvement, they are all within the protection scope of the present invention.

Claims

1. An SPR sensor, characterized in that: The invention comprises a base (1), a reaction device (2) is arranged at the bottom of the base (1), a prism (31) is arranged at the top of the base (1), an incident light source device (5) is arranged on one side of the prism (31), the incident light source device (5) comprises a laser (51), and a reflection receiving device (6) is arranged on the other side of the prism (31), the reflection receiving device (6) comprises a receiver (61).

2. An SPR sensor according to claim 1, characterized in that: Polarizing plates (56) and condensing lenses (57) are symmetrically arranged on both sides of the prism (31).

3. An SPR sensor according to claim 1, characterized in that: A convex lens (55) is arranged on one side of the condenser (57) located at the position of the incident light source device (5).

4. An SPR sensor according to claim 2, characterized in that: A protective light shield (3) is arranged outside the prism (31), a through hole is provided on the top of the protective light shield (3), and an LED light source (4) is installed in the through hole.

5. An SPR sensor according to claim 4, characterized in that: One side of the protective light shield (3) is in communication with one end of a third light shielding protective tube (62), and the other end of the third light shielding protective tube (62) is in communication with a receiver (61).

6. The SPR sensor according to claim 5, characterized in that: The other side of the protective shading cover (3) is connected to one end of a second shading protection tube (54), the other end of the second shading protection tube (54) is slidably connected to one end of an adjustable shading protection tube (53), the other end of the adjustable shading protection tube (53) is slidably connected to one end of a first shading protection tube (52), the other end of the first shading protection tube (52) is connected to a laser (51), and the inner circle of the adjustable shading protection tube (53) is fixedly connected to the outer circle of a convex lens (55).

7. The SPR sensor according to claim 1, characterized in that: The reaction device (2) comprises a gold film (21), a prism (31) is arranged at the upper end of the gold film (21), the gold film (21) is installed in a slot provided in the middle of the base (1), an inlet plate (22) is arranged at the lower end of the gold film (21), the inlet plate (22) is slidably connected to an adjustment plate (23), one side of the adjustment plate (23) is an inclined surface, and the other side of the adjustment plate (23) is a hand-push end.

8. An SPR detection device, characterized in that: The SPR detection device comprises the modulated SPR sensor according to any one of claims 1 to 7.

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